Portable nucleic acid detection device
By designing the main body and detection component structure of a portable nucleic acid detection device, and combining it with the design of photoelectric devices, the problems of high cost and low sensitivity were solved, achieving efficient and low-cost nucleic acid detection results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- DAAN GENE CO LTD
- Filing Date
- 2025-04-28
- Publication Date
- 2026-04-28
AI Technical Summary
Existing portable nucleic acid testing devices are costly, have low sensitivity, and are not convenient enough.
A portable nucleic acid detection device was designed, including a main body and a detection component. The main body is provided with a liquid inlet channel, a detection chamber and an insertion channel. The detection component is movably inserted into the insertion channel. The contact time and area with the mixed solution are adjusted by controlling the movement of the detection component in the insertion channel. Combined with the design that does not require photoelectric devices, the sensitivity is improved.
It enables nucleic acid detection that is simple to operate, low in cost, and highly sensitive, and requires no external power supply. It has a wide range of applications and high detection efficiency.
Smart Images
Figure CN224172754U_ABST
Abstract
Description
Technical Field
[0001] This application relates to the field of biodetection technology, and more specifically, to a portable nucleic acid detection device. Background Technology
[0002] In recent years, the types of infectious diseases threatening human life have been increasing, and nucleic acid testing, as a crucial detection method, has become increasingly important. However, existing nucleic acid testing equipment is bulky and inconvenient for individuals to carry and use on-site. Therefore, there is an urgent need for a portable nucleic acid testing device.
[0003] Currently, portable nucleic acid testing devices have several problems: First, some portable nucleic acid testing devices still rely on external power drivers for contact between the internal detection element and / or the liquid to be tested, resulting in limited applicability and low convenience; second, existing portable nucleic acid testing devices usually involve the acquisition and analysis of fluorescence signals, resulting in relatively high testing costs; and third, portable nucleic acid testing devices cannot freely control the degree of contact between the detection element and the liquid to be tested during use, leading to low detection sensitivity.
[0004] In summary, existing portable nucleic acid testing devices suffer from high cost, low sensitivity, and low convenience. Utility Model Content
[0005] The technical problem to be solved by the embodiments of this application is that the existing portable nucleic acid detection devices have problems such as high cost, low sensitivity and low convenience.
[0006] To solve the above-mentioned technical problems, the embodiments of this application adopt the following solutions:
[0007] A portable nucleic acid testing device, the portable nucleic acid testing device comprising:
[0008] The main body is provided with a liquid inlet channel, a detection chamber and an insertion channel connected in sequence. The liquid inlet channel is connected to the external environment and is used to place reactants and allow external liquid to enter the detection chamber. The insertion channel is connected to the external environment.
[0009] A detection component, which is movably inserted into the insertion channel, with at least a portion of the detection component located within the detection cavity.
[0010] Furthermore, the detection component includes a fixing member, a sliding member, and a detection member. The fixing member is connected to the detection member. The sliding member is located within the insertion channel and is used to slide along the length direction of the insertion channel. The sliding member is provided with a mounting hole along the length direction of the insertion channel. The fixing member is connected to the end of the mounting hole away from the insertion channel. At least a portion of the detection member is located within the mounting hole.
[0011] Furthermore, the fastener has a first gap on the side facing the insertion channel. The size of the first gap gradually increases along the direction from the fastener to the insertion channel. The detection element is snapped into the first gap and is interference-fitted with the first gap.
[0012] Furthermore, the fixing member has a first elastic protrusion and a second elastic protrusion on the side facing the insertion channel, forming the first gap between the first elastic protrusion and the second elastic protrusion. Both the first elastic protrusion and the second elastic protrusion are located within the mounting hole and are interference-fitted with the mounting hole; and / or,
[0013] The outer wall of the slider is provided with an elastic ring, and the elastic ring and the insertion channel are interference-fitted.
[0014] Furthermore, there are multiple insertion channels, detection cavities, and detection components, and each insertion channel, detection cavity, and detection component is configured in a one-to-one correspondence.
[0015] The liquid inlet channel includes a first liquid inlet channel, a second liquid inlet channel, and a plurality of third liquid inlet channels connected in sequence. The first liquid inlet channel, the second liquid inlet channel, and the third liquid inlet channels are respectively located on opposite sides of the main body. The first liquid inlet channel is connected to the external environment, and the third liquid inlet channels are connected to the detection chamber one by one.
[0016] The connection between the first liquid inlet channel and the second liquid inlet channel is a liquid inlet. The third liquid inlet channel includes a first sub-channel, a second sub-channel, and a third sub-channel connected in sequence. The first sub-channel is connected to the end of the second liquid inlet channel away from the liquid inlet. Along the direction from the liquid inlet away from the first liquid inlet channel, the distance between the second sub-channel and the liquid inlet is A, where A > 0. The third sub-channel is connected to the detection chamber.
[0017] Furthermore, the first sub-channel is also equipped with a capillary valve.
[0018] Furthermore, the first sub-channel is also provided with a reaction chamber located between the capillary valve and the second inlet channel, the reaction chamber being used to place the reactants; and / or,
[0019] The main body includes a base and a cover plate, the cover plate being connected to the main body to seal the liquid inlet channel and the detection chamber.
[0020] Furthermore, the main body is provided with a transparent portion, and the detection cavity is disposed in the transparent portion; and / or,
[0021] The portable nucleic acid testing device also includes a sealing membrane that covers the end of the liquid inlet channel away from the main body.
[0022] Furthermore, the portable nucleic acid detection device also includes a hydrophobic and breathable membrane. The main body is provided with an installation groove, and the installation groove is provided with a vent hole. The vent hole connects the detection chamber and the external environment. The hydrophobic and breathable membrane is disposed in the installation groove to seal the vent hole.
[0023] Furthermore, the pore size of the hydrophobic and breathable membrane ranges from 0.01 μm to 50 μm.
[0024] Compared with the prior art, the embodiments of this application have the following main advantages:
[0025] Users can allow the detection solution to enter the main body through the inlet channel and mix with the reactants to form a first mixture. The first mixture is then heated to carry out the amplification reaction. After the amplification reaction is completed, diluent is injected to form a second mixture. After the second mixture enters the detection chamber, users can control the degree to which the detection component enters the detection chamber to change the contact time and contact area between the detection component and the second mixture. Therefore, the portable nucleic acid detection device has high sensitivity. Attached Figure Description
[0026] To more clearly illustrate the solutions in this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.
[0027] Figure 1 This is a schematic diagram of the structure of the portable nucleic acid detection device according to an embodiment of this application;
[0028] Figure 2 This is another structural schematic diagram of the portable nucleic acid detection device according to an embodiment of this application;
[0029] Figure 3 Figure 1 is a schematic diagram of the portable nucleic acid testing device according to another embodiment of this application.
[0030] Figure label:
[0031] 1. Main body; 2. Detection chamber; 3. Vent hole; 4. Detection element; 5. Insertion channel; 6. Sliding element; 7. Fixing element; 8. First liquid inlet channel; 9. First sub-channel; 10. Third sub-channel; 11. Reaction chamber; 12. Capillary valve; 13. Liquid inlet; 14. Cover plate; 15. Elastic ring; 16. Hydrophobic and breathable membrane; 17. Mounting groove; 18. Second liquid inlet channel; 19. Second sub-channel; 20. First elastic protrusion; 21. Second elastic protrusion; 22. First gap; 23. Mounting hole. Detailed Implementation
[0032] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application. Furthermore, it should be understood that the specific embodiments described herein are only for illustration and explanation of this application and are not intended to limit this application.
[0033] In this application, unless otherwise stated, directional terms such as "upper" and "lower" generally refer to the upper and lower positions of the device in its actual use or operating state, specifically the orientation shown in the accompanying drawings; while "inner" and "outer" refer to the outline of the device. Furthermore, in the description of this application, the term "comprising" means "including but not limited to". The terms first, second, third, etc., are used merely as illustrative purposes and do not impose numerical requirements or establish a numerical order.
[0034] In this application, "and / or" describes the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. A and B can be singular or plural.
[0035] In this application, "at least one" means one or more, and "more than one" means two or more. "At least one," "at least one of the following," or similar expressions refer to any combination of these items, including any combination of single or multiple items. For example, "at least one of a, b, or c," or "at least one of a, b, and c," can both mean: a, b, c, ab (i.e., a and b), ac, bc, or abc, where a, b, and c can be single or multiple.
[0036] Various embodiments of this application may exist in the form of a range; it should be understood that the description in the form of a range is merely for convenience and brevity and should not be construed as a hard limitation on the scope of this application; therefore, it should be considered that the range description has specifically disclosed all possible sub-ranges and single numerical values within that range. For example, it should be considered that the range description from 1 to 6 has specifically disclosed sub-ranges such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., and single numbers within the range, such as 1, 2, 3, 4, 5, and 6, regardless of the range. Furthermore, whenever a numerical range is referred to herein, it means including any referenced number (fraction or integer) within the referred range.
[0037] Please refer to Figures 1 to 2 This application provides a portable nucleic acid testing device, which includes:
[0038] The main body 1 is provided with a liquid inlet channel, a detection chamber 2 and an insertion channel 5 connected in sequence. The liquid inlet channel is connected to the external environment and is used to place reactants and allow external liquid to enter the detection chamber 2. The insertion channel 5 is connected to the external environment.
[0039] The detection component is movably inserted into the insertion channel 5, with at least a portion of the detection component located within the detection cavity 2.
[0040] In this embodiment, the portable nucleic acid detection device is used as follows: First, a detection solution (including nucleic acid solution) is added to the inlet channel. The detection solution contacts the reactants to form a first mixed solution. Then, the first mixed solution is heated to allow PCR (polymerase chain reaction, amplification reaction) to occur between the detection solution and the reactants in the first mixed solution. Second, after the amplification reaction is completed, a diluent (including elution buffer, distilled water, and DNA dissolving solution) is added to the inlet channel. The diluent dilutes the first mixed solution to form a second mixed solution, ensuring that the nucleic acid after PCR is at a suitable DNA template concentration for subsequent detection. Then, after the second mixed solution enters the detection chamber 2, the user can control the insertion of the detection component into the channel 5 and move it toward the detection chamber 2 so that the detection component contacts the second mixed solution as needed and obtains the detection result. According to actual needs, the user can control the degree to which the detection component enters the detection chamber 2 to change the contact time and contact area between the detection component and the second mixed solution, thus the portable nucleic acid detection device has high sensitivity.
[0041] In summary, the portable nucleic acid testing device of this application embodiment is simple to operate and does not require the installation of photoelectric devices, thus it has low production cost, high efficiency and high sensitivity.
[0042] It should be understood that the reactants include lyophilized bulbs and lyophilized powders, and their components are usually various enzymes, solutions, ions and primers / probes for nucleic acid detection.
[0043] Further, please refer to Figures 1 to 2 The detection assembly includes a fixing member 7, a sliding member 6, and a detection member 4. The fixing member 7 is connected to the detection member 4. The sliding member 6 is located in the insertion channel 5 and is used to slide along the length direction of the insertion channel 5. The sliding member 6 is provided with a mounting hole 23 along the length direction of the insertion channel 5. The fixing member 7 is connected to the end of the mounting hole 23 away from the insertion channel 5. At least part of the detection member 4 is located in the mounting hole 23.
[0044] In this embodiment, the length direction of the insertion channel 5 is the Z direction in the figure. The slider 6 can move along the length direction of the insertion channel 5 to protect the test piece 4 from impact with the insertion channel 5 (for example, to prevent the test strip from bending and deforming when directly inserted into the insertion channel 5), so that the test piece 4 can smoothly enter the detection chamber 2. The connection between the fixing member 7 and the test piece 4 allows the user to control the depth of the test piece 4 in the mounting hole 23, thereby controlling the degree to which the test piece 4 enters the detection chamber 2, and improving the sensitivity of the portable nucleic acid detection device. It should be understood that the test piece 4 may include a test strip, which can be immersed in the second mixed solution for chromatography to read the test results, and the nucleic acid test results are reflected by color changes, the appearance of stripes, and other changes.
[0045] Further, please refer to Figure 2 The fixing member 7 is provided with a first gap 22 on the side facing the insertion channel 5. The size of the first gap 22 gradually increases along the direction from the fixing member 7 to the insertion channel 5. The detection member 4 is snapped into the first gap 22 and is interference-fitted with the first gap 22.
[0046] In this embodiment, when the detection element 4 is inserted into the first gap 22 along the direction from the insertion channel 5 to the fixing element 7, it will gradually be clamped by the first gap 22, and the clamping force will gradually increase as the detection element 4 moves. Therefore, the portable nucleic acid detection device of this embodiment can quickly adjust the connection stability between the detection element 4 and the fixing element 7 by adjusting the degree to which the detection element 4 enters the first gap 22, and can also quickly remove and replace the detection element 4 after completing the nucleic acid test, thereby improving the replacement efficiency of the portable nucleic acid detection device.
[0047] Further, please refer to Figure 2 The fastener 7 is provided with a first elastic protrusion 20 and a second elastic protrusion 21 on the side facing the insertion channel 5. A first gap 22 is formed between the first elastic protrusion 20 and the second elastic protrusion 21. The first elastic protrusion 20 and the second elastic protrusion 21 are both located in the mounting hole 23 and are interference fit with the mounting hole 23.
[0048] In this embodiment, when the detection element 4 is located within the first gap 22 and the fixing element 7 is inserted into the mounting hole 23, the first elastic protrusion 20 and the second elastic protrusion 21 will close together because they are in interference fit with the mounting hole 23, thereby further clamping the detection element 4 and improving the connection stability between the detection element 4 and the fixing element 7.
[0049] Further, please refer to Figure 2 The outer wall of the sliding member 6 is provided with an elastic ring 15, and the elastic ring 15 and the insertion channel 5 are interference-fitted.
[0050] In this embodiment, the elastic ring 15 can be press-fitted with the insertion channel 5 to seal the insertion channel 5 and prevent the second mixture in the detection cavity 2 from overflowing from the insertion channel 5 into the body 1.
[0051] Furthermore, nucleic acid testing usually needs to be performed multiple times, or simultaneously using multiple testing components, to compare results and ensure the accuracy of the nucleic acid test.
[0052] Please refer to Figure 1 and Figure 2 There are multiple insertion channels 5, detection chambers 2 and detection components, and each insertion channel 5, detection chamber 2 and detection component is set in a one-to-one correspondence.
[0053] The liquid inlet channel includes a first liquid inlet channel 8, a second liquid inlet channel 18, and multiple third liquid inlet channels connected in sequence. The first liquid inlet channel 8, the second liquid inlet channel 18, and the third liquid inlet channels are respectively located along the main body 1. Figure 2 On opposite sides in the Y direction, the first liquid inlet channel 8 is connected to the external environment, and the third liquid inlet channel is connected to the detection chamber 2 in a corresponding manner.
[0054] The connection between the first liquid inlet channel 8 and the second liquid inlet channel 18 is the liquid inlet 13. The third liquid inlet channel includes a first sub-channel 9, a second sub-channel 19 and a third sub-channel 10 connected in sequence. The first sub-channel 9 is connected to the end of the second liquid inlet channel 18 away from the liquid inlet 13. Along the direction from the liquid inlet 13 away from the first liquid inlet channel 8, the distance between the second sub-channel 19 and the liquid inlet 13 is A, where A > 0. The third sub-channel 10 is connected to the detection chamber 2.
[0055] In this embodiment, the distance between the second sub-channel 19 and the inlet 13 should be understood as the distance along the Z direction in the figure, and the straight line formed by this distance is parallel to the Z direction. The first inlet channel 8 is located on the side of the main body 1 where the third inlet channel is not provided, and the second inlet channel 18 and the third inlet channel are located on the same side of the main body 1. The detection liquid will first enter the first inlet channel 8, and then enter the second inlet channel 18. Then the detection liquid will be divided into multiple streams and flow into different third inlet channels respectively. Finally, multiple detection chambers 2 contain the second mixed solution. The user can control multiple detection components to contact the second mixed solution in different detection chambers 2 respectively, so as to complete multiple nucleic acid tests simultaneously or in batches. Then, based on the detection results of multiple detection components, the final result of nucleic acid testing can be evaluated to improve the accuracy of nucleic acid testing.
[0056] Meanwhile, since the distance between the second sub-channel 19 and the inlet 13 is A, and A > 0, a height difference is formed between the second sub-channel 19 and the inlet 13. At this time, when the detection liquid enters the second sub-channel 19 from bottom to top (along the inlet 13 to the direction away from the first inlet channel 8, Z direction in the figure), based on the pressure, the second mixed solution will first fill the reaction chambers in the first sub-channel 9 of the different third inlet channels under the action of the height difference, so that the reactants in each reaction chamber 11 can be soaked by the same volume of detection liquid, so as to avoid the difference in results caused by uneven distribution of detection liquid.
[0057] It should be understood that when there are two detection chambers 2, two insertion channels 5, two third liquid inlet channels, and two detection components, the structure of the portable nucleic acid detection device is as follows: Figure 1 As shown, its detection chamber 2, insertion channel 5, third liquid inlet channel and detection component are symmetrically distributed, and its axis of symmetry is the central axis of the first liquid inlet channel 8.
[0058] Further, please refer to Figure 1 The first sub-channel 9 is also equipped with a capillary valve 12.
[0059] In this embodiment, the capillary valve 12 can further slow down the flow rate of the detection liquid before it enters the second sub-channel 19, so as to further ensure that the detection liquid can be evenly distributed into different reaction chambers 11.
[0060] Further, please refer to Figure 1 The first sub-channel 9 is also provided with a reaction chamber 11 located between the capillary valve 12 and the second liquid inlet channel 18. The reaction chamber 11 is used to place the reactants.
[0061] In this embodiment, the reaction chamber 11 is capable of accommodating the reactants. The size of the reaction chamber 11 is adapted to the detection liquid. In this case, after the user adds the detection liquid, the first mixed solution generated by mixing the detection liquid and the reactants remains in the reaction chamber 11, rather than entering the second sub-channel 19, thereby preventing the undiluted first mixed solution from directly entering the detection chamber 2.
[0062] Further, please refer to Figure 2 and Figure 3 The main body 1 includes a base and a cover plate 14. The cover plate 14 is connected to the main body 1 to seal the liquid inlet channel and the detection chamber 2.
[0063] In this embodiment, the second liquid inlet channel 18, the third liquid inlet channel, and the reaction chamber 11 are all formed in the form of grooves on the main body 1. The cover plate 14 can then seal these grooves to form the second liquid inlet channel 18, the third liquid inlet channel, and the reaction chamber 11. During the production of the portable nucleic acid testing device, the main body 1 and the cover plate 14 can be quickly assembled to improve the production efficiency of the portable nucleic acid testing device. It should be understood that the cover plate 14 can be double-sided tape, a film, or a plastic sheet.
[0064] Further, please refer to Figure 1 and Figure 3 The main body 1 has a transparent part (not marked in the figure), and the detection cavity 2 is located in the transparent part.
[0065] In this embodiment, because the detection chamber 2 is located in the transparent section, the user can observe the contact between the detection component and the second mixed solution through the transparent section, and simultaneously observe the detection results detected by the detection component, without having to pull the detection component out and insert it into the channel 5. Therefore, the portable detection device of this embodiment has high detection efficiency.
[0066] Further, please refer to Figure 1 The portable nucleic acid testing device also includes a sealing membrane (not marked in the figure), which covers the end of the liquid inlet channel away from the main body 1.
[0067] In this embodiment, the sealing membrane can be an aluminum membrane, which can effectively seal the liquid inlet channel, preventing external moisture from entering the liquid inlet channel and reacting with the reactants, thereby improving the nucleic acid detection efficiency of the portable nucleic acid detection device.
[0068] Further, please refer to Figure 3 The portable nucleic acid testing device also includes a hydrophobic and breathable membrane 16. The main body 1 is provided with an installation groove 17, and an air vent 3 is provided in the installation groove 17. The air vent 3 connects the detection chamber 2 and the external environment. The hydrophobic and breathable membrane 16 is provided in the installation groove 17 to seal the air vent 3.
[0069] In this embodiment, the mounting groove 17 can prevent the hydrophobic and breathable membrane 16 from shifting to a preset position. The combination of the vent 3 and the hydrophobic and breathable membrane 16 can prevent external liquid from entering the detection chamber 2, while ensuring that the gas inside the detection chamber 2 leaves through the vent 3, so as to ensure the gas pressure balance inside the detection chamber 2, thereby allowing the second mixed solution to enter the detection chamber 2 smoothly.
[0070] Further, please refer to Figure 3 The pore size of the hydrophobic and breathable membrane 16 ranges from 0.01 μm to 50 μm.
[0071] In this embodiment, the pore size range of the hydrophobic and breathable membrane 16 is 0.01 μm to 50 μm. Within this range, the hydrophobic and breathable membrane 16 can ensure good isolation of liquids while improving gas permeability. The pore size range of the hydrophobic and breathable membrane 16 can be 0.01 μm, 1 μm, 2 μm, 3 μm, 4 μm, 5 μm, 6 μm, 7 μm, 8 μm, 9 μm, 10 μm, 11 μm, 12 μm, 13 μm, 14 μm, 15 μm, 16 μm, 17 μm, 18 μm, 1... The range of any value or any two values between 9μm, 20μm, 21μm, 22μm, 23μm, 24μm, 25μm, 26μm, 27μm, 28μm, 29μm, 30μm, 31μm, 32μm, 33μm, 34μm, 35μm, 36μm, 37μm, 38μm, 39μm, 40μm, 41μm, 42μm, 43μm, 44μm, 45μm, 46μm, 47μm, 48μm, 49μm, and 50μm. It should be understood that the hydrophobic and breathable membrane 16 can be installed using methods such as double-sided adhesive, ultrasonic welding, and laser welding.
[0072] Obviously, the embodiments described above are only some embodiments of this application, not all embodiments. The accompanying drawings show preferred embodiments of this application, but do not limit the patent scope of this application. This application can be implemented in many different forms; rather, the purpose of providing these embodiments is to provide a more thorough and comprehensive understanding of the disclosure of this application. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing specific embodiments, or make equivalent substitutions for some of the technical features. Any equivalent structures made using the content of this application's specification and drawings, directly or indirectly applied to other related technical fields, are similarly within the scope of patent protection of this application.
[0073] Although embodiments of this application have been shown and described, those skilled in the art will understand that various changes, modifications, combinations, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of this application, the scope of which is defined by the claims and their equivalents.
Claims
1. A portable nucleic acid detection device, characterized in that, The portable nucleic acid testing device includes: The main body is provided with a liquid inlet channel, a detection chamber and an insertion channel connected in sequence. The liquid inlet channel is connected to the external environment and is used to place reactants and allow external liquid to enter the detection chamber. The insertion channel is connected to the external environment. A detection component, which is movably inserted into the insertion channel, with at least a portion of the detection component located within the detection cavity.
2. The portable nucleic acid detection device according to claim 1, characterized in that, The detection component includes a fixing member, a sliding member, and a detection member. The fixing member is connected to the detection member. The sliding member is located in the insertion channel and is used to slide along the length direction of the insertion channel. The sliding member is provided with a mounting hole along the length direction of the insertion channel. The fixing member is connected to the end of the mounting hole away from the insertion channel. At least part of the detection member is located in the mounting hole.
3. The portable nucleic acid detection device according to claim 2, characterized in that, The fastener has a first gap on the side facing the insertion channel. The size of the first gap gradually increases along the fastener toward the insertion channel. The detection element is snapped into the first gap and is interference-fitted with the first gap.
4. The portable nucleic acid detection device according to claim 3, characterized in that, The fastener has a first elastic protrusion and a second elastic protrusion on the side facing the insertion channel, forming the first gap between the first elastic protrusion and the second elastic protrusion. Both the first elastic protrusion and the second elastic protrusion are located within the mounting hole and are interference-fitted with the mounting hole; and / or, The outer wall of the slider is provided with an elastic ring, and the elastic ring and the insertion channel are interference-fitted.
5. The portable nucleic acid detection device according to claim 1, characterized in that, There are multiple insertion channels, detection cavities, and detection components, and each insertion channel, detection cavity, and detection component is configured in a one-to-one correspondence. The liquid inlet channel includes a first liquid inlet channel, a second liquid inlet channel, and a plurality of third liquid inlet channels connected in sequence. The first liquid inlet channel, the second liquid inlet channel, and the third liquid inlet channels are located on opposite sides of the main body. The first liquid inlet channel is connected to the external environment, and the third liquid inlet channels are connected to the detection chamber one by one. The connection between the first liquid inlet channel and the second liquid inlet channel is a liquid inlet. The third liquid inlet channel includes a first sub-channel, a second sub-channel, and a third sub-channel connected in sequence. The first sub-channel is connected to the end of the second liquid inlet channel away from the liquid inlet. Along the direction from the liquid inlet away from the first liquid inlet channel, the distance between the second sub-channel and the liquid inlet is A, where A > 0. The third sub-channel is connected to the detection chamber.
6. The portable nucleic acid detection device according to claim 5, characterized in that, The first sub-channel is also equipped with a capillary valve.
7. The portable nucleic acid detection device according to claim 6, characterized in that, The first sub-channel is further provided with a reaction chamber located between the capillary valve and the second inlet channel, the reaction chamber being used to place the reactants; and / or, The main body includes a base and a cover plate, the cover plate being connected to the main body to seal the liquid inlet channel and the detection chamber.
8. The portable nucleic acid detection device according to claim 1, characterized in that, The main body is provided with a transparent portion, and the detection cavity is disposed in the transparent portion; and / or, The portable nucleic acid testing device also includes a sealing membrane that covers the end of the liquid inlet channel away from the main body.
9. The portable nucleic acid detection device according to claim 1, characterized in that, The portable nucleic acid detection device also includes a hydrophobic and breathable membrane. The main body is provided with an installation groove, and the installation groove is provided with a vent hole. The vent hole connects the detection chamber and the external environment. The hydrophobic and breathable membrane is disposed in the installation groove to seal the vent hole.
10. The portable nucleic acid detection device according to claim 9, characterized in that, The pore size of the hydrophobic and breathable membrane ranges from 0.01 μm to 50 μm.