Conversion device for converting lipid into phospholipid type DHA (docosahexaenoic acid) lipid through enzyme catalysis

Abstract

The utility model discloses a conversion device for converting phospholipid type DHA (docosahexaenoic acid) grease by enzyme catalysis, which comprises a sample storage device, an oil outlet pipeline, an oil return pipeline, a plurality of oil pipelines and a plurality of columnar reaction containers, the first columnar reaction container is a water removal column, and the other columnar reaction containers are enzyme reaction columns; the sample storage device is provided with an oil outlet and an oil return port, the top of the columnar reaction container is an oil inlet end, and the bottom of the columnar reaction container is an oil outlet end; the oil outlet of the sample storage device is communicated with the oil inlet end of the first columnar reaction container through an oil outlet pipeline, and the oil outlet end of the last columnar reaction container is communicated with the oil return opening of the sample storage device through an oil return pipeline; in two adjacent columnar reaction containers, the oil outlet end of the previous columnar reaction container is communicated with the oil inlet end of the next columnar reaction container through a corresponding oil conveying pipeline; an oil outlet pump is arranged on the oil outlet pipeline, and an oil conveying pump is arranged on the oil conveying pipeline. The device disclosed by the utility model realizes full contact between grease and enzyme and meets the requirements of industrial continuous production.

Description

Technical Field

[0001] This utility model relates to the field of oil processing technology, and in particular to an enzyme-catalyzed conversion device for oil to phospholipid-type DHA oil. Background Technology

[0002] DHA (docosahexaenoic acid), as an essential fatty acid, plays a vital role in human health, especially in the growth and development of infants and young children. Phospholipids regulate metabolism and enhance physical performance; lecithin plays a crucial role in the body's metabolic processes. In recent years, the functions of phospholipids have received increasing attention, demonstrating good effects in regulating metabolism, enhancing physical performance, strengthening brain function, and regulating blood lipids, leading to their wide application in various fields. Phospholipids are a general term for a class of phosphate-containing lipids, essential components of cell membranes, nuclear membranes, and plasmid membranes in animals and plants, and are one of the fundamental substances of life, possessing significant nutritional and medicinal value.

[0003] The enzymatic preparation of phospholipid-type DHA is typically carried out in non-aqueous reaction solvent systems, mainly including organic solvent systems and solvent-free systems, and currently, the reaction conditions are limited to shaker-flask setups. Traditional shaker-flask methods rely on the manual addition of desiccants or simple filtration to remove water, making it impossible to precisely control the moisture content of the reaction system. This leads to unstable phospholipase activity, and residual moisture can easily cause side reactions. Furthermore, mixing in the shaker flask relies solely on shaking, resulting in insufficient contact efficiency between DHA lipids and enzymes, long reaction times, and large batch-to-batch conversion rate fluctuations, failing to meet the stability requirements of industrial production. Utility Model Content

[0004] The problem to be solved by this utility model is to provide an enzyme-catalyzed conversion device for oil to phospholipid-type DHA. This device can automatically adsorb and remove water, reduce the water content of the system, avoid the inhibition of phospholipase activity by water, reduce hydrolysis side reactions, and achieve full contact between oil and enzyme to meet the needs of continuous industrial production.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0006] A device for the enzyme-catalyzed conversion of lipids into phospholipid-type DHA lipids is characterized by comprising a sample storage container, an oil outlet pipe, an oil return pipe, multiple oil delivery pipes, and multiple column-shaped reaction vessels. The first column-shaped reaction vessel is a dehydration column, and the remaining column-shaped reaction vessels are enzyme reaction columns. The sample storage container is provided with an oil outlet and an oil return port. The top of the column-shaped reaction vessels is the oil inlet, and the bottom of the column-shaped reaction vessels is the oil outlet. The oil outlet of the sample storage container is connected to the oil inlet of the first column-shaped reaction vessel through the oil outlet pipe, and the oil outlet of the last column-shaped reaction vessel is connected to the oil return port of the sample storage container through the oil return pipe. In two adjacent column-shaped reaction vessels, the oil outlet of the first column-shaped reaction vessel is connected to the oil inlet of the second column-shaped reaction vessel through a corresponding oil delivery pipe. An oil outlet pump is provided on the oil outlet pipe, and an oil delivery pump is provided on the oil delivery pipe. Both the oil outlet pump and the oil delivery pump are peristaltic pumps.

[0007] The adsorption and dehydration material in the above-mentioned dehydration column is a molecular sieve (such as a zeolite molecular sieve).

[0008] The enzyme reaction columns described above contain enzymes, typically immobilized enzymes. The number of enzyme reaction columns can be flexibly set according to the required amount of oil and fat reaction.

[0009] For enzyme catalysis, the mixed oils are first stored in a sample storage container. The oil pump is then turned on, and the oil from the sample storage container is transported through the oil outlet pipe to a dehydration column for adsorption and dehydration, reducing the system's water content and preventing water from inhibiting phospholipase activity, while also minimizing hydrolysis side reactions. Once the oil in the dehydration column has accumulated to a suitable level, the corresponding oil pump is turned on, and the oil from the dehydration column is transported through the oil outlet pipe to the first enzyme reaction column for enzyme reaction. When the oil in the first enzyme reaction column has accumulated to a suitable level, the corresponding oil pump is turned on, and the oil from the first enzyme reaction column is transported through the oil outlet pipe to the next enzyme reaction column. When the oil in the last enzyme reaction column has accumulated to a suitable level, the oil from the last enzyme reaction column is transported back to the sample storage container through the oil return pipe. This completes one cycle, and the cycle is repeated to ensure that the DHA oil and enzymes react fully, thereby obtaining phospholipid-type DHA oil, which can further advance the industrialization process of enzyme-catalyzed oil-to-phospholipid conversion.

[0010] In a preferred embodiment, both the oil outlet pipe and the oil delivery pipe are equipped with a first switching valve. The switching valves can be used to control the on / off state of the oil outlet pipe and the oil delivery pipe as needed for the reaction.

[0011] In a preferred embodiment, the return oil pipeline is equipped with a return oil pump, which is a peristaltic pump.

[0012] In a further preferred embodiment, a second switching valve is provided on the oil return pipeline.

[0013] In a preferred embodiment, the enzyme-catalyzed conversion device for phospholipid-type DHA lipids further includes a circulating insulation system. This system comprises an insulated water tank, a temperature controller, an outlet pipe, a return pipe, multiple connecting pipes, an electric heating device for heating the insulated water, and a temperature sensor for detecting the water temperature. The sample storage container and each of the column-shaped reaction vessels have an insulated jacketed cavity on their outer walls. Each insulated jacketed cavity has an inlet and an outlet. The outlet of the insulated water tank is connected to the inlet of the insulated jacketed cavity of the last column-shaped reaction vessel via an outlet pipe. The outlet of the insulated jacket cavity of the reactor is connected to the inlet of the insulated jacket cavity of the preceding cylindrical reaction vessel via a connecting pipe. The outlet of the insulated jacket cavity of the first cylindrical reaction vessel is connected to the inlet of the insulated jacket cavity of the sample storage vessel via a connecting pipe. The outlet of the insulated jacket cavity of the sample storage vessel is connected to the return port of the insulated water tank via a return water pipe. A third switch valve is installed on the outlet pipe. The electric heating device, temperature sensor, and temperature controller are all installed on the insulated water tank. The temperature sensor is electrically connected to the corresponding signal input terminal of the temperature controller, and the electric heating device is electrically connected to the corresponding signal output terminal of the temperature controller. The above-described circulating insulation system can achieve heat reuse and reduce energy consumption. While the enzyme catalysis is underway, the preheated circulating insulation system should be turned on. The water temperature in the insulation tank is controlled by the temperature controller, electric heating device, and temperature sensor to maintain a suitable temperature (e.g., 50°C). The insulation water is then sequentially transported through the outlet pipe and connecting pipes to the insulation jacket cavity of each column-shaped reaction vessel and sample storage container. Finally, the insulation water is transported back to the insulation tank through the return pipe. This process keeps the oils in each column-shaped reaction vessel and sample storage container warm, ensuring that the enzyme catalysis of the oil circulation reaction takes place under constant temperature conditions, thus ensuring stable enzyme activity and improving reaction consistency.

[0014] In another preferred embodiment, the enzyme-catalyzed conversion device for phospholipid-type DHA lipids further includes a circulating insulation system. This system comprises an insulated water tank, a temperature controller, an outlet pipe, a return pipe, an electric heating device for heating the insulated water, and a temperature sensor for detecting the water temperature. The outer walls of the sample storage container and each of the cylindrical reaction vessels are equipped with insulated jacketed cavities. Each insulated jacketed cavity has an inlet and an outlet. The inlets of the insulated jacketed cavities of each cylindrical reaction vessel and sample storage container are connected to the outlet of the insulated water tank via outlet pipes. The outlets of the insulated jacketed cavities of each cylindrical reaction vessel and sample storage container are connected to the return outlet of the insulated water tank via return pipes. A third switching valve is installed on the outlet pipe. The electric heating device, temperature sensor, and temperature controller are all mounted on the insulated water tank. The temperature sensor is electrically connected to the corresponding signal input terminal of the temperature controller, and the electric heating device is electrically connected to the corresponding signal output terminal of the temperature controller. This circulating insulation system enables heat reuse and reduces energy consumption. While the enzyme catalysis is underway, the preheated circulating insulation system should be turned on. The water temperature in the insulation tank is controlled by the cooperation of the temperature controller, electric heating device and temperature sensor to maintain the water temperature at a suitable temperature (e.g. 50℃). The insulation water is then sequentially transported through the outlet pipe to the insulation jacket cavity of each column-shaped reaction vessel and sample storage container, and then transported back to the insulation tank through the return pipe. This process keeps the oils in each column-shaped reaction vessel and sample storage container warm, ensuring that the enzyme catalysis of the oil circulation reaction takes place under constant temperature conditions, thus ensuring stable enzyme activity and improving reaction consistency.

[0015] Compared with the prior art, this utility model has the following advantages:

[0016] This invention can reduce the water content of the system, avoid the inhibition of phospholipase activity by water, reduce hydrolysis side reactions, achieve full contact between oil and enzyme, avoid the low efficiency of single-reaction shaking flask, meet the different needs of different customers for DHA oil absorption and utilization quality, and increase the market value of DHA oil to meet the needs of continuous industrial production. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of a specific embodiment 1 of this utility model;

[0018] Figure 2 This is a structural schematic diagram of a specific embodiment 2 of the present invention. Detailed Implementation

[0019] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments.

[0020] Example 1, such as Figure 1As shown, the enzyme-catalyzed conversion device for phospholipid-type DHA oil in this embodiment includes a sample storage container 1, an oil outlet pipe 2, an oil return pipe 3, multiple oil delivery pipes 4, and multiple column-shaped reaction vessels 5. The first column-shaped reaction vessel 5 is a dehydration column 51, and the remaining column-shaped reaction vessels 5 are enzyme reaction columns 52. The sample storage container 1 is provided with an oil outlet 11 and an oil return port 12. The top of the column-shaped reaction vessels 5 is the oil inlet, and the bottom of the column-shaped reaction vessels 5 is the oil outlet. Oil outlet 11 is connected to the oil inlet of the first column-shaped reaction vessel 5 through oil outlet pipe 2, and the oil outlet of the last column-shaped reaction vessel 5 is connected to the oil return port 12 of the sample storage vessel 1 through oil return pipe 3; in two adjacent column-shaped reaction vessels 5, the oil outlet of the first column-shaped reaction vessel 5 is connected to the oil inlet of the second column-shaped reaction vessel 5 through the corresponding oil delivery pipe 4; oil outlet pipe 2 is equipped with oil outlet pump 6, and oil delivery pipe 4 is equipped with oil delivery pump 7. Both oil outlet pump 6 and oil delivery pump 7 are peristaltic pumps.

[0021] The adsorption and dehydration material in the above-mentioned dehydration column 51 is a molecular sieve (such as a zeolite molecular sieve).

[0022] The enzyme reaction column 52 described above contains an enzyme, typically an immobilized enzyme. The number of enzyme reaction columns 52 can be flexibly set according to the required amount of oil reaction.

[0023] To perform enzyme catalysis, the mixed oils are first stored in sample storage container 1. The oil pump 6 is then turned on, and the oil in sample storage container 1 is transported through oil outlet pipe 2 to dehydration column 51 for adsorption and dehydration, reducing the water content of the system and preventing water from inhibiting phospholipase activity, while also reducing hydrolysis side reactions. When the oil in dehydration column 51 accumulates to a suitable amount, the corresponding oil pump 7 is turned on, and the oil in dehydration column 51 is transported through oil outlet pipe 4 to the first enzyme reaction column 52 for enzyme reaction. When the oil in the first enzyme reaction column 52 accumulates... When the appropriate amount is reached, the corresponding oil pump 7 is turned on to transfer the oil in the first enzyme reaction column 52 to the next enzyme reaction column 52 through the oil pipeline 4; when the oil in the last enzyme reaction column 52 accumulates to an appropriate amount, the oil in the last enzyme reaction column 52 is transferred to the sample storage container 1 through the return oil pipeline 3; after the above reaction is completed, it is a cycle, and this cycle reaction is carried out to allow the DHA oil and enzyme to react fully, thereby obtaining phospholipid-type DHA oil, which can further promote the industrialization process of enzyme-catalyzed oil to phospholipid conversion reaction.

[0024] Both the oil outlet pipe 2 and the oil delivery pipe 4 are equipped with a first switching valve 8. The switching valves can be used to control the on / off state of the oil outlet pipe 2 and the oil delivery pipe 4 as needed for the reaction.

[0025] The return oil pipeline 3 is equipped with a return oil pump 9, which is a peristaltic pump. The return oil pipeline 3 is also equipped with a second switch valve 10.

[0026] This enzyme-catalyzed conversion device for lipids to phospholipid-type DHA also includes a circulating insulation system 20. The circulating insulation system 20 includes an insulated water tank 201, a temperature controller 202, an outlet pipe 203, a return pipe 204, multiple connecting pipes 205, an electric heating device (not shown in the figure) for heating the insulated water, and a temperature sensor (not shown in the figure) for detecting the temperature of the insulated water. The sample storage container 1 and each of the column-shaped reaction vessels 5 have an insulated jacket cavity 13 on their outer walls. Each insulated jacket cavity 13 has an inlet and an outlet. The outlet of the insulated water tank 201 is connected to the inlet of the insulated jacket cavity 13 of the last column-shaped reaction vessel 5 via the outlet pipe 203. The outlet of the insulated jacket cavity 13 is connected to the inlet of the insulated jacket cavity 13 of the previous cylindrical reaction vessel 5 via a connecting pipe 205. The outlet of the insulated jacket cavity 13 of the first cylindrical reaction vessel 5 is connected to the inlet of the insulated jacket cavity 13 of the sample storage vessel 1 via a connecting pipe 205. The outlet of the insulated jacket cavity 13 of the sample storage vessel 1 is connected to the return port of the insulated water tank 201 via a return water pipe 204. A third switch valve 206 is provided on the outlet pipe 203. The electric heating device, temperature sensor, and temperature controller 202 are all installed on the insulated water tank 201. The temperature sensor is electrically connected to the corresponding signal input terminal of the temperature controller 202, and the electric heating device is electrically connected to the corresponding signal output terminal of the temperature controller 202. The above-mentioned circulating insulation system 20 can realize the reuse of heat and reduce energy consumption. While the enzyme catalysis is underway, the preheated circulating insulation system 20 is turned on. The water temperature in the insulation water tank 201 is controlled by the temperature controller 202, the electric heating device, and the temperature sensor to maintain the water temperature in the insulation water tank 201 at a suitable temperature (e.g., 50°C). The insulation water is then sequentially transported through the outlet pipe 203 and each connecting pipe 205 to the insulation jacket cavity 13 of each column-shaped reaction vessel 5 and sample storage container 1. The insulation water is then transported back to the insulation water tank 201 through the return water pipe 204. This process keeps the oils in each column-shaped reaction vessel 5 and sample storage container 1 warm, ensuring that the enzyme catalysis of the oil circulation reaction takes place under constant temperature conditions, thus ensuring stable enzyme activity and improving reaction consistency.

[0027] Example 2, as Figure 2 As shown, the difference between this embodiment and Embodiment 1 is that:

[0028] This enzyme-catalyzed conversion device for lipids to phospholipid-type DHA lipids also includes a circulating heat preservation system 20. The circulating heat preservation system 20 includes a heat preservation water tank 201, a temperature controller 202, an outlet water pipe 203, a return water pipe 204, an electric heating device for heating the heat preservation water, and a temperature sensor for detecting the temperature of the heat preservation water. The outer walls of the sample storage container 1 and each of the column-shaped reaction vessels 5 are provided with heat-insulated interlayer cavities 13. Each heat-insulated interlayer cavity 13 is provided with an inlet and an outlet. All 13 inlets are connected to the outlet of the insulated water tank 201 via outlet pipes 203. The outlets of the insulated jacket cavities 13 of each of the column-shaped reaction vessels 5 and sample storage containers 1 are connected to the return outlet of the insulated water tank 201 via return pipes 204. A third switch valve 206 is installed on the outlet pipe 203. An electric heating device, a temperature sensor, and a temperature controller 202 are all installed on the insulated water tank 201. The temperature sensor is electrically connected to the corresponding signal input terminal of the temperature controller 202, and the electric heating device is electrically connected to the corresponding signal output terminal of the temperature controller 202. This circulating insulation system 20 enables heat reuse and reduces energy consumption. While the enzyme catalysis is underway, the preheated circulating insulation system 20 is turned on. The water temperature in the insulation water tank 201 is controlled by the temperature controller 202, the electric heating device, and the temperature sensor to maintain the water temperature in the insulation water tank 201 at a suitable temperature (e.g., 50°C). The insulation water is then sequentially transported through the outlet pipe 203 to the insulation jacket cavity 13 of each column-shaped reaction vessel 5 and sample storage container 1. The insulation water is then transported back to the insulation water tank 201 through the return water pipe 204. This process keeps the oils in each column-shaped reaction vessel 5 and sample storage container 1 warm, ensuring that the enzyme catalysis of the oil circulation reaction takes place under constant temperature conditions, thus ensuring stable enzyme activity and improving reaction consistency.

[0029] Furthermore, it should be noted that the names of the various parts of the specific embodiments described in this specification may differ. All equivalent or simple variations made to the structure, features, and principles described in this utility model patent concept are included within the protection scope of this utility model patent. Those skilled in the art to which this utility model pertains may make various modifications or additions to the described specific embodiments or use similar methods to replace them, as long as they do not deviate from the structure of this utility model or exceed the scope defined in these claims, they should all fall within the protection scope of this utility model.

Claims

1. A device for enzyme-catalyzed conversion of lipids into phospholipid-type DHA lipids, characterized in that: The system includes a sample storage container, an oil outlet pipe, an oil return pipe, multiple oil delivery pipes, and multiple column-shaped reaction vessels. The first column-shaped reaction vessel is a dehydration column, and the remaining column-shaped reaction vessels are enzyme reaction columns. The sample storage container has an oil outlet and an oil return port. The top of the column-shaped reaction vessels is the oil inlet, and the bottom of the column-shaped reaction vessels is the oil outlet. The oil outlet of the sample storage container is connected to the oil inlet of the first column-shaped reaction vessel through an oil outlet pipe, and the oil outlet of the last column-shaped reaction vessel is connected to the oil return port of the sample storage container through an oil return pipe. In two adjacent column-shaped reaction vessels, the oil outlet of the first column-shaped reaction vessel is connected to the oil inlet of the second column-shaped reaction vessel through a corresponding oil delivery pipe. An oil outlet pump is installed on the oil outlet pipe, and an oil delivery pump is installed on the oil delivery pipe. Both the oil outlet pump and the oil delivery pump are peristaltic pumps.

2. The enzyme-catalyzed conversion device for converting lipids into phospholipid-type DHA lipids as described in claim 1, characterized in that: Both the oil outlet pipe and the oil delivery pipe are equipped with a first switching valve.

3. The enzyme-catalyzed conversion device for converting lipids into phospholipid-type DHA lipids as described in claim 1 or 2, characterized in that: The return oil pipeline is equipped with a return oil pump, which is a peristaltic pump.

4. The enzyme-catalyzed conversion device for converting lipids into phospholipid-type DHA lipids as described in claim 3, characterized in that: A second switching valve is installed on the return oil pipeline.

5. The enzyme-catalyzed conversion device for oil to phospholipid-type DHA as described in claim 1, characterized in that: It also includes a circulating heat preservation system, which comprises a heat preservation water tank, a temperature controller, an outlet water pipe, a return water pipe, multiple connecting pipes, an electric heating device for heating the heat preservation water, and a temperature sensor for detecting the temperature of the heat preservation water. The sample storage container and the outer walls of each cylindrical reaction vessel are provided with heat preservation jacket cavities. Each heat preservation jacket cavity has an inlet and an outlet. The outlet of the heat preservation water tank is connected to the inlet of the heat preservation jacket cavity of the last cylindrical reaction vessel via an outlet pipe. The outlet of the heat preservation jacket cavity of the subsequent cylindrical reaction vessel is connected via a connecting pipe. The pipeline is connected to the inlet of the insulated jacket cavity of the first column-shaped reaction vessel. The outlet of the insulated jacket cavity of the first column-shaped reaction vessel is connected to the inlet of the insulated jacket cavity of the sample storage vessel via a connecting pipeline. The outlet of the insulated jacket cavity of the sample storage vessel is connected to the return port of the insulated water tank via a return water pipeline. A third switch valve is installed on the outlet pipeline. The electric heating device, temperature sensor, and temperature controller are all installed on the insulated water tank. The temperature sensor is electrically connected to the corresponding signal input terminal of the temperature controller, and the electric heating device is electrically connected to the corresponding signal output terminal of the temperature controller.

6. The enzyme-catalyzed conversion device for oil to phospholipid-type DHA as described in claim 1, characterized in that: It also includes a circulating heat preservation system, which comprises a heat preservation water tank, a temperature controller, an outlet pipe, a return pipe, an electric heating device for heating the heat preservation water, and a temperature sensor for detecting the temperature of the heat preservation water. The outer walls of the sample storage container and each of the cylindrical reaction vessels are provided with heat preservation jacket cavities. Each heat preservation jacket cavity is provided with an inlet and an outlet. The inlets of the heat preservation jacket cavities of each cylindrical reaction vessel and sample storage container are connected to the outlet of the heat preservation water tank through the outlet pipe. The outlets of the heat preservation jacket cavities of each cylindrical reaction vessel and sample storage container are connected to the return outlet of the heat preservation water tank through the return pipe. A third switch valve is provided on the outlet pipe. The electric heating device, temperature sensor, and temperature controller are all installed on the heat preservation water tank. The temperature sensor is electrically connected to the corresponding signal input terminal of the temperature controller, and the electric heating device is electrically connected to the corresponding signal output terminal of the temperature controller.

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