Full-automatic integrated digital PCR analyzer

Abstract

The utility model relates to the technical field of in-vitro diagnosis, and provides a full-automatic integrated digital PCR analyzer which comprises a shell, a chip warehousing module, a chip carrying module, a PCR amplification module and a chip reading module, and a normally-closed cabin door is arranged on the shell; the normally-closed bin door, the chip bin entering module and the PCR amplification module are sequentially and adjacently arranged in the first direction, the chip carrying module and the chip reading module are adjacently arranged in the first direction, the chip reading module and the chip bin entering module are adjacently arranged in the second direction, and the chip carrying module and the PCR amplification module are adjacently arranged in the second direction; the chip warehouse entering module is used for bearing a chip carrier and conveying the chip carrier into the shell or conveying the chip carrier out of the shell through the normally-closed warehouse door. The chip carrying module is used for clamping the chip carrier to move in the first direction, the second direction or the third direction, and the first direction, the second direction and the third direction are perpendicular to one another. The device is compact in structure, achieves full-automatic operation, and improves the experiment efficiency.

Description

A fully automated integrated digital PCR analyzer Technical Field

[0001] This application relates to the field of in vitro diagnostic technology, and in particular to a fully automated integrated digital PCR analyzer. Background Technology

[0002] Digital PCR (dPCR) is a third-generation PCR technology that achieves absolute quantitative detection at the single-molecule level by precisely micro-partitioning nucleic acid samples. Its core principle involves uniformly distributing the sample to be tested into tens of thousands of independent microreaction units (such as droplets or microwells). After PCR amplification, the original concentration is directly calculated by counting the number of positive units containing the target nucleic acid and using a Poisson distribution algorithm. Compared to traditional quantitative real-time PCR (qPCR), digital PCR does not rely on a standard curve, has higher sensitivity (detecting mutation frequencies as low as 0.001%), and stronger anti-interference capabilities. It is particularly suitable for the precise detection of low-abundance nucleic acids, rare mutations, and copy number variations, demonstrating unique advantages in areas such as tumor liquid biopsy, pathogen load analysis, and gene editing validation.

[0003] However, existing instruments or devices for digital PCR are all separate, consisting of a sample loading device, an amplification device, and a reading device. Operators need to manually handle the chip at each step and manually transfer the chip after each step, which leads to low experimental efficiency. Summary of the Invention

[0004] To address the aforementioned problems, this application provides a fully automated integrated digital PCR analyzer. This analyzer has a compact structure, requires no manual operation, achieves fully automated operation, and improves experimental efficiency. The technical solution adopted in this application is as follows:

[0005] A fully automated integrated digital PCR analyzer includes: a housing, a chip loading module, a chip transport module, a PCR amplification module, and a chip reading module. The housing is provided with a normally closed door, and the chip loading module, the chip transport module, the PCR amplification module, and the chip reading module are all located inside the housing.

[0006] The normally closed chamber door, the chip loading module, and the PCR amplification module are arranged adjacent to each other in a first direction. The chip transport module and the chip reading module are arranged adjacent to each other in a first direction. The chip reading module and the chip loading module are arranged adjacent to each other in a second direction. The chip transport module and the PCR amplification module are arranged adjacent to each other in a second direction.

[0007] The chip loading module is used to carry the chip carrier and transport the chip carrier into the housing or to the outside of the housing through the normally closed hatch door.

[0008] The chip handling module is used to clamp the chip carrier and move it along the first direction, the second direction or the third direction, and the first direction, the second direction and the third direction are perpendicular to each other.

[0009] The application has a compact structure and a reasonable layout. After placing the chip to be amplified in the chip loading module, no manual operation is required. The amplification of the chip and the analysis after chip amplification are automatically completed by this analyzer, achieving full-automatic operation and improving the experimental efficiency. By setting the normally closed hatch door, the risk of the analyzer being interfered by the external environment during operation is reduced.

[0010] In some embodiments, the chip loading module includes a first driving component and a chip carrier. The chip carrier is slidably connected to the first driving component, and the first driving component can drive the chip carrier to slide along the first direction. When the chip carrier slides away from the PCR amplification module, the chip carrier can push open the normally closed hatch door and extend out of the housing, so that an operator can place the chip carrier on the chip carrier. When the chip carrier slides towards the PCR amplification module, the normally closed hatch door can automatically close.

[0011] By pushing or not pushing the normally closed hatch door with the chip carrier, the opening and closing of the normally closed hatch door are realized. When it is necessary to place the chip carrier on the chip carrier or remove the chip carrier from the chip carrier, the hatch door can be opened by pushing the normally closed hatch door with the chip carrier, realizing the linkage control of the normally closed hatch door and the chip carrier. There is no need to set other components specifically for controlling the opening of the normally closed hatch door. When the chip carrier no longer pushes the normally closed hatch door, the normally closed hatch door automatically closes. This technical solution for realizing the opening and closing of the normally closed hatch door is simple, reliable and low-cost.

[0012] In some embodiments, the first driving component includes a first driving motor, a first guide rail and a first transmission belt. The first guide rail is arranged along the first direction. The first driving motor is arranged at the bottom of the first guide rail. The first transmission belt is arranged along the length direction of the first guide rail. The first driving motor can drive the first transmission belt to rotate reciprocally. The chip carrier is slidably connected to the first guide rail and the chip carrier is also connected to the first transmission belt, so that the first transmission belt drives the chip carrier to move along the first direction.

[0013] In some embodiments, the chip handling module includes a second driving component, a second and third direction driving component and a driving clamping component;

[0014] The second driving component includes a second driving motor, a second guide rail, and a second transmission belt. The second guide rail is arranged along the first direction and located above the chip reading module. The second transmission belt is arranged along the length direction of the second guide rail and located below the second guide rail. The second driving motor can drive the second transmission belt to reciprocate.

[0015] The second-direction third-direction driving component is slidably connected to the second guide rail and suspended below the second guide rail. The second-direction third-direction driving component is also connected to the second transmission belt so that the second transmission belt drives the second-direction third-direction driving component to move along the first direction. The second-direction third-direction driving component is used to drive the driving clamping component to move along the second direction and the third direction. The driving clamping component is used to clamp the chip carrier.

[0016] By placing the second-direction third-direction drive component and the chip reading module below the second guide rail, the overall dimensions of the analyzer can be effectively controlled.

[0017] In some embodiments, the third-direction drive assembly in the second direction includes a third drive assembly and a fourth drive assembly. The third drive assembly is slidably connected to the second guide rail and suspended below the second guide rail. The third drive assembly is also connected to the second transmission belt so that the second transmission belt drives the third drive assembly to move along the first direction. The third drive assembly is used to drive the fourth drive assembly to move along the second direction, and the fourth drive assembly is used to drive the drive clamping assembly to move along the third direction.

[0018] In some embodiments, the fourth drive assembly includes a telescopic drive assembly and a fourth guide rail. The actuating end of the telescopic drive assembly is capable of telescopic extension and retraction along the third direction. The fourth guide rail is arranged along the third direction. The drive clamping assembly is slidably connected to the fourth guide rail and connected to the actuating end of the telescopic drive assembly.

[0019] In some embodiments, the telescopic drive assembly is a telescopic motor.

[0020] In some embodiments, the drive clamping assembly includes a fifth drive motor, a fifth guide rail, a cam, two clamping arms, and a reset assembly; the fifth guide rail is arranged along the first direction, the two clamping arms are slidably connected to the fifth guide rail, the two clamping arms abut against opposite sides of the outer periphery of the cam, the fifth drive motor is used to drive the cam to rotate so that the two clamping arms move away from each other along the first direction, and the reset assembly is used to drive the two clamping arms to move closer to each other along the first direction.

[0021] By setting a cam and a reset component, simultaneous control of the movement of two clamping arms by a driving motor is achieved, thus making the structure of the driving clamping component simple.

[0022] In some embodiments, the cam is elliptical.

[0023] By designing the cam to be elliptical, during the process of using the rotation of the cam to control the two clamping arms to move away from or close to each other, the two clamping arms can move smoothly and steadily, reducing the risk of tremors when picking up and placing the chip carrier.

[0024] In some embodiments, the reset component includes a spring, and both ends of the spring are respectively connected to one and the other of the two clamping arms.

[0025] By respectively connecting both ends of the spring to one and the other of the two clamping arms, self - reset of the two clamping arms is achieved, thus greatly simplifying the structure of the reset component.

[0026] The full - automatic integrated digital PCR analyzer provided by the present application has at least one of the following beneficial effects:

[0027] 1. The full - automatic integrated digital PCR analyzer provided by the present application has a compact structure and reasonable layout. After placing the chip to be amplified in the chip loading module, no manual operation is required, and the amplification of the chip and the analysis after chip amplification are automatically completed by the analyzer, achieving full - automatic operation and improving the experimental efficiency. By setting a normally - closed door, the risk of interference from the external environment during the operation of the analyzer is reduced.

[0028] 2. The full - automatic integrated digital PCR analyzer provided by the present application realizes the opening and closing of the normally - closed door by whether the chip carrier rack pushes the normally - closed door or not. When it is necessary to place the chip carrier on the chip carrier rack or take the chip carrier off the chip carrier rack, the normally - closed door can be opened by the chip carrier rack pushing the normally - closed door, realizing the linkage control of the normally - closed door and the chip carrier rack. There is no need to set other components specifically for controlling the opening of the normally - closed door. When the chip carrier rack no longer pushes the normally - closed door, the normally - closed door automatically closes. This technical solution for realizing the opening and closing of the normally - closed door is simple, reliable, and has a low cost.

[0029] 3. The full - automatic integrated digital PCR analyzer provided by the present application can effectively control the overall external dimension of the analyzer by arranging both the second - direction and third - direction driving components and the chip reading module below the second guide rail.

[0030] 4. The fully automatic integrated digital PCR analyzer provided in this application realizes the simultaneous control of the movement of two clamping arms by a single drive motor through the setting of a cam and a reset component, thus simplifying the structure of the drive clamping component.

[0031] 5. The fully automated integrated digital PCR analyzer provided in this application, by designing the cam as an ellipse, allows the two clamping arms to move smoothly and stably during the process of controlling the two clamping arms to move away from or towards each other by rotating the cam, thereby reducing the risk of vibration of the clamping arms when picking up and placing the chip carrier.

[0032] 6. The fully automated integrated digital PCR analyzer provided in this application achieves self-resetting of the two clamping arms by connecting the two ends of the spring to one and the other of the two clamping arms respectively, which greatly simplifies the construction of the reset component. Attached Figure Description

[0033] The preferred embodiments of a fully automated integrated digital PCR analyzer will be further explained below in a clear and easy-to-understand manner, with reference to the accompanying drawings, to illustrate the above-mentioned characteristics, technical features, advantages, and implementation methods of the analyzer:

[0034] Figure 1 is a schematic diagram of the overall appearance of the fully automated integrated digital PCR analyzer;

[0035] Figure 2 is an internal structure diagram of an embodiment of a fully automated integrated digital PCR analyzer;

[0036] Figure 3 is another perspective of the embodiment in Figure 2;

[0037] Figure 4 is a top view of the embodiment in Figure 2;

[0038] Figure 5 is a side view of the embodiment in Figure 2;

[0039] Figure 6 is another side view of the embodiment in Figure 2;

[0040] Figure 7 is a schematic diagram of the chip loading module;

[0041] Figure 8 is a schematic diagram of the chip carrier structure;

[0042] Figure 9 is a schematic diagram of the structure of the first drive component;

[0043] Figure 10 is a schematic diagram of the chip handling module;

[0044] Figure 11 is a schematic diagram of the structure of the second drive component;

[0045] Figure 12 is a schematic diagram of the structure of the chip handling module;

[0046] Figure 13 is a schematic diagram of the structure of the third drive module;

[0047] Figure 14 is a schematic diagram of the structure of the fourth drive assembly and the drive clamping assembly;

[0048] Figure 15 is another perspective of the embodiment in Figure 14;

[0049] Figure 16 is a schematic diagram of the PCR amplification module;

[0050] Figure 17 is a schematic diagram of the chip reading module;

[0051] Figure 18 is a schematic diagram of the structure of a chip carrier (carrying a chip).

[0052] Explanation of icon numbers:

[0053] 1. Housing; 2. Normally closed door; 3. Chip loading module; 4. First drive assembly; 5. First drive motor; 6. First guide rail; 7. First transmission belt; 8. Chip carrier; 9. Chip handling module; 10. Second drive assembly; 11. Second drive motor; 12. Second guide rail; 13. Second transmission belt; 14. Third drive assembly; 15. Third drive motor; 16. Third guide rail; 17. Third transmission belt; 18. Chip carrier; 19. Chip carrier; 20. Chip loading module; 10. First drive assembly; 11. First transmission belt; 19. Third drive assembly; 10. Third drive motor; 11. Third guide rail; 12. Third transmission belt; 19. Fourth drive assembly; 10. Fourth drive assembly; 11. Third transmission belt; 19. Fourth drive assembly; 10. Fourth transmission belt; 19. Fourth drive assembly; 10. Fourth telescopic drive assembly; 11. Fourth guide rail; 19. Fourth transmission belt; 10. Fourth drive assembly; 19. Fourth telescopic drive assembly; 10. Fourth telescopic drive assembly; 19. Fourth telescopic drive assembly; 10. Fourth telescopic drive assembly; 19. Fourth telescopic drive assembly; 10. Fourth telescopic drive assembly; 19. Fourth telescopic drive assembly; 10. Fourth telescopic drive assembly; 19. Fifth drive motor; 19. Fifth guide rail; 19. Fifth drive motor; 10. Fifth guide rail; 19. Fourth transmission belt; 19. Fifth drive assembly; 10. Fifth drive motor; 19 ... Detailed Implementation

[0054] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the specific implementation methods of this application will be described below with reference to the accompanying drawings. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings and other implementation methods can be obtained based on these drawings without creative effort.

[0055] To keep the drawings concise, each drawing only schematically shows the parts relevant to this application, and they do not represent the actual structure of the product. Furthermore, for ease of understanding, in some drawings, only one of the components with the same structure or function is schematically shown, or only one is labeled. In this document, "one" not only means "only one," but can also mean "more than one."

[0056] It should also be further understood that the term “and / or” as used in this application specification and the appended claims means any combination of one or more of the associated listed items and all possible combinations, and includes such combinations.

[0057] In this document, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections, detachable connections, or integral connections; they can refer to mechanical connections or electrical connections; they can refer to direct connections or indirect connections through an intermediate medium; and they can refer to the internal connection between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0058] Furthermore, in the description of this application, the terms "first," "second," etc., are used only to distinguish descriptions and should not be construed as indicating or implying relative importance.

[0059] Referring to Figures 1-18, this application provides a fully automated integrated digital PCR analyzer, including: a housing 1, a chip loading module 3, a chip transport module 4, a PCR amplification module 5, and a chip reading module 6. The housing 1 is provided with a normally closed door 2, and the chip loading module 3, the chip transport module 4, the PCR amplification module 5, and the chip reading module 6 are all located inside the housing 1.

[0060] The normally closed chamber door 2, the chip loading module 3 and the PCR amplification module 5 are arranged adjacent to each other along the first direction. The chip transport module 4 and the chip reading module 6 are arranged adjacent to each other along the first direction. The chip reading module 6 and the chip loading module 3 are arranged adjacent to each other along the second direction. The chip transport module 4 and the PCR amplification module 5 are arranged adjacent to each other along the second direction.

[0061] The chip loading module 3 is used to carry the chip carrier 7 and transport the chip carrier 7 into the housing 1 or to the outside of the housing 1 through the normally closed door 2;

[0062] The chip handling module 4 is used to clamp the chip carrier 7 and move it along a first direction, a second direction, or a third direction, wherein the first direction, the second direction, and the third direction are perpendicular to each other.

[0063] This application features a compact structure and reasonable layout. After placing the chip to be amplified in the chip loading module 3, no manual operation is required. The chip amplification and post-amplification analysis are both automatically completed by the analyzer, achieving fully automated operation and improving experimental efficiency. By setting a normally closed door 2, the risk of external environmental interference during the analyzer's operation is reduced.

[0064] It is understood that the setting of the analysis parameters and the presentation of the analysis results of this analyzer require the use of peripheral devices or instruments. This application does not describe the peripheral devices or instruments, but only focuses on how to realize the chip loading, amplification and analysis.

[0065] It should be noted beforehand that, referring to Figures 2-6, 16, and 17, the chip reading module 6 includes an analysis window 61, which faces upwards. After the chip transport module 4 transports the chip carrier 7 containing the chip onto the analysis window 61, the chip reading module 6 can analyze the chip. Additionally, the PCR amplification module 5 includes a carrier position 51, a capping assembly 52, and a heat dissipation assembly 53. After the chip transport module 4 places the chip carrier 7 containing the chip onto the carrier position 51, the capping assembly 52 automatically caps the chip carrier 7. The capping assembly 52 contains a heating element, and the carrier position 51 contains a Peltier. By controlling the current, the Peltier can be heated or cooled. The heat dissipation assembly 53 is located below the carrier position 51. By controlling the heating element, the Peltier, and the heat dissipation assembly 53, temperature regulation (heating or cooling) can be achieved, thereby enabling chip amplification. It should be noted that there is at least one set of PCR amplification modules 5. That is to say, there can be one, two, three or more sets of PCR amplification modules 5. In this application, two sets of PCR amplification modules 5 are shown.

[0066] Referring to Figures 2-9, in one embodiment, the chip loading module 3 includes a first driving component 31 and a chip carrier 32. The chip carrier 32 is slidably connected to the first driving component 31, and the first driving component 31 can drive the chip carrier 32 to slide along a first direction. When the chip carrier 32 slides away from the PCR amplification module 5, the chip carrier 32 can push open the normally closed door 2 and extend it outside the housing 1 so that the operator can place the chip carrier 7 in the chip carrier 32. When the chip carrier 32 slides towards the PCR amplification module 5, the normally closed door 2 can automatically close.

[0067] It is worth noting that the normally closed compartment door 2 is opened and closed by pushing it against the chip carrier 32. When it is necessary to place the chip carrier 7 onto the chip carrier 32 or to remove the chip carrier 7 from the chip carrier 32, the compartment door is opened by pushing it against the chip carrier 32. This achieves linkage control between the normally closed compartment door 2 and the chip carrier 32, eliminating the need for other components specifically designed to control the opening of the normally closed compartment door 2. When the chip carrier 32 stops pushing the normally closed compartment door 2, it automatically closes. This technical solution for opening and closing the normally closed compartment door 2 is simple, reliable, and low in cost. It is also worth noting that the chip carrier 32 can hold one, two, three, or more chip carriers 7. This application demonstrates a chip carrier 32 that can hold two chip carriers 7.

[0068] Referring to Figures 2-9, in one embodiment, the first driving component 31 includes a first driving motor 311, a first guide rail 312, and a first transmission belt 313. The first guide rail 312 is arranged along a first direction, the first driving motor 311 is located at the bottom of the first guide rail 312, and the first transmission belt 313 is arranged along the length of the first guide rail 312. The first driving motor 311 can drive the first transmission belt 313 to reciprocate. The chip carrier 32 is slidably connected to the first guide rail 312 and is also connected to the first transmission belt 313, so that the first transmission belt 313 drives the chip carrier 32 to move along the first direction.

[0069] Referring to Figures 2-6 and 10-15, in one embodiment, the chip handling module 4 includes a second driving component 41, a second-direction third-direction driving component, and a driving clamping component 44;

[0070] The second drive assembly 41 includes a second drive motor 411, a second guide rail 412, and a second transmission belt 413. The second guide rail 412 is arranged along the first direction and is located above the chip reading module 6. The second transmission belt 413 is arranged along the length direction of the second guide rail 412 and is located below the second guide rail 412. The second drive motor 411 can drive the second transmission belt 413 to reciprocate.

[0071] The second-direction third-direction drive component is slidably connected to the second guide rail 412 and suspended below the second guide rail 412. The second-direction third-direction drive component is also connected to the second transmission belt 413 so that the second transmission belt 413 drives the second-direction third-direction drive component to move along the first direction. The second-direction third-direction drive component is used to drive the drive clamping component 44 to move along the second direction and the third direction. The drive clamping component 44 is used to clamp the chip carrier 7.

[0072] It is easy to understand that by placing both the second-direction third-direction drive component and the chip reading module 6 below the second guide rail 412, the overall size of the analyzer can be effectively controlled.

[0073] Referring to Figures 2-6 and 10-15, in one embodiment, the third-direction driving component in the second direction includes a third driving component 42 and a fourth driving component 43. The third driving component 42 is slidably connected to the second guide rail 412 and suspended below the second guide rail 412. The third driving component 42 is also connected to the second transmission belt 413 so that the second transmission belt 413 drives the third driving component 42 to move in the first direction. The third driving component 42 is used to drive the fourth driving component 43 to move in the second direction, and the fourth driving component 43 is used to drive the driving clamping component 44 to move in the third direction.

[0074] Referring to Figures 2-6 and 10-15, in one embodiment, the fourth drive component 43 includes a telescopic drive component 431 and a fourth guide rail 432. The actuator of the telescopic drive component 431 is capable of telescopic extension and retraction along a third direction. The fourth guide rail 432 is arranged along a third direction. The drive clamping component 44 is slidably connected to the fourth guide rail 432 and connected to the actuator of the telescopic drive component 431.

[0075] Referring to Figures 2-6 and 10-15, in one embodiment, the telescopic drive assembly 431 is a telescopic motor. In other embodiments, the telescopic drive assembly 431 may be a linear cylinder.

[0076] Referring to Figures 2-6 and 10-15, in one embodiment, the drive clamping assembly 44 includes a fifth drive motor 441, a fifth guide rail 442, a cam 443, two clamping arms 444, and a reset assembly. The fifth guide rail 442 is arranged along a first direction, and the two clamping arms 444 are slidably connected to the fifth guide rail 442. The two clamping arms 444 abut against opposite sides of the outer periphery of the cam 443. The fifth drive motor 441 is used to drive the cam 443 to rotate so that the two clamping arms 444 move away from each other along the first direction. The reset assembly is used to drive the two clamping arms 444 to move closer to each other along the first direction.

[0077] It is worth noting that by setting up the cam 443 and the reset component, a single drive motor can simultaneously control the movement of the two clamping arms 444, thus simplifying the construction of the drive clamping component 44.

[0078] Referring to Figures 10, 12, 14, and 15, in one embodiment, the cam 443 is elliptical. By designing the cam 443 as elliptical, the two gripping arms 444 can move smoothly and steadily during the process of controlling the two gripping arms 444 to move away from or towards each other using the rotation of the cam 443, reducing the risk of vibration of the gripping arms 444 when picking up and placing the chip carrier 7. In other embodiments, the cam 443 can be rhomboid.

[0079] Referring to Figure 15, in one embodiment, the reset assembly includes a spring 445, the two ends of which are respectively connected to one and the other of the two clamping arms 444. By connecting the two ends of the spring 445 to one and the other of the two clamping arms 444, the self-resetting of the two clamping arms 444 is achieved, thus greatly simplifying the construction of the reset assembly.

[0080] Referring to Figures 10, 12, and 13, in one embodiment, the third drive assembly 42 includes a third drive motor 421, a third guide rail 422, and a third transmission belt 423. The third guide rail 422 is arranged along the second direction and is slidably suspended below the second guide rail 412. The third transmission belt 423 is arranged along the length of the third guide rail 422. The third drive motor 421 can drive the third transmission belt 423 to reciprocate.

[0081] It should be noted that the above embodiments can be freely combined as needed. The above are merely preferred embodiments of this application. It should be pointed out that for those skilled in the art, several improvements and modifications can be made without departing from the principles of this application, and these improvements and modifications should also be considered within the scope of protection of this application.

Claims

1. A fully automated integrated digital PCR analyzer, characterized in that, include: The device comprises a housing, a chip loading module, a chip transport module, a PCR amplification module, and a chip reading module. The housing has a normally closed door. The chip loading module, chip transport module, PCR amplification module, and chip reading module are all located within the housing. The normally closed door, chip loading module, and PCR amplification module are arranged adjacent to each other along a first direction. The chip transport module and chip reading module are arranged adjacent to each other along the first direction. The chip reading module and chip loading module are arranged adjacent to each other along a second direction. The chip transport module and PCR amplification module are arranged adjacent to each other along the second direction. The chip loading module carries the chip carrier and transports the chip carrier into or out of the housing through the normally closed door. The chip transport module clamps the chip carrier and moves it along the first direction, the second direction, or a third direction, wherein the first direction, the second direction, and the third direction are perpendicular to each other.

2. The fully automated integrated digital PCR analyzer according to claim 1, characterized in that, The chip loading module includes a first driving component and a chip carrier. The chip carrier is slidably connected to the first driving component, and the first driving component can drive the chip carrier to slide along the first direction. When the chip carrier slides away from the PCR amplification module, the chip carrier can push open the normally closed door and extend it outside the housing, so that the operator can place the chip carrier in the chip carrier. When the chip carrier slides toward the PCR amplification module, the normally closed compartment door can automatically close.

3. The fully automated integrated digital PCR analyzer according to claim 2, characterized in that, The first driving component includes a first driving motor, a first guide rail, and a first transmission belt. The first guide rail is arranged along the first direction, the first driving motor is located at the bottom of the first guide rail, and the first transmission belt is arranged along the length of the first guide rail. The first driving motor can drive the first transmission belt to reciprocate. The chip carrier is slidably connected to the first guide rail and is also connected to the first transmission belt, so that the first transmission belt drives the chip carrier to move along the first direction.

4. The fully automated integrated digital PCR analyzer according to claim 1, characterized in that, The chip handling module includes a second driving component, a second-direction third-direction driving component, and a driving clamping component. The second driving component includes a second driving motor, a second guide rail, and a second transmission belt. The second guide rail is arranged along the first direction and located above the chip reading module. The second transmission belt is arranged along the length of the second guide rail and located below the second guide rail. The second driving motor can drive the second transmission belt to reciprocate. The second-direction third-direction driving component is slidably connected to the second guide rail and suspended below the second guide rail. The second-direction third-direction driving component is also connected to the second transmission belt so that the second transmission belt drives the second-direction third-direction driving component to move along the first direction. The second-direction third-direction driving component is used to drive the driving clamping component to move along the second direction and the third direction. The driving clamping component is used to clamp the chip carrier.

5. The fully automated integrated digital PCR analyzer according to claim 4, characterized in that, The third-direction drive assembly in the second direction includes a third drive assembly and a fourth drive assembly. The third drive assembly is slidably connected to the second guide rail and suspended below the second guide rail. The third drive assembly is also connected to the second transmission belt so that the second transmission belt drives the third drive assembly to move along the first direction. The third drive assembly is used to drive the fourth drive assembly to move along the second direction, and the fourth drive assembly is used to drive the drive clamping assembly to move along the third direction.

6. The fully automated integrated digital PCR analyzer according to claim 5, characterized in that, The fourth drive component includes a telescopic drive component and a fourth guide rail. The actuator of the telescopic drive component is capable of telescopic extension and retraction along the third direction. The fourth guide rail is arranged along the third direction. The drive clamping component is slidably connected to the fourth guide rail and connected to the actuator of the telescopic drive component.

7. The fully automated integrated digital PCR analyzer according to claim 6, characterized in that, The telescopic drive component is a telescopic motor.

8. A fully automated integrated digital PCR analyzer according to any one of claims 4-7, characterized in that, The drive clamping assembly includes a fifth drive motor, a fifth guide rail, a cam, two clamping arms, and a reset assembly. The fifth guide rail is arranged along the first direction, and the two clamping arms are slidably connected to the fifth guide rail. The two clamping arms abut against opposite sides of the outer periphery of the cam. The fifth drive motor is used to drive the cam to rotate so that the two clamping arms move away from each other along the first direction. The reset assembly is used to drive the two clamping arms to move closer to each other along the first direction.

9. The fully automated integrated digital PCR analyzer according to claim 8, characterized in that, The cam is elliptical.

10. A fully automated integrated digital PCR analyzer according to claim 8, characterized in that, The reset assembly includes a spring, the two ends of which are respectively connected to one and the other of the two clamping arms.

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