Automatic sample adding device for DNA fragmentation rate detection

Abstract

The utility model relates to the technical field of medical instruments, in particular to a DNA fragmentation rate detection automatic sample adding device which comprises a machine shell, a temperature control box is installed at the bottom end in the machine shell, an installation plate is fixedly installed at the top of the temperature control box, and a mechanical rotating mechanism is installed at the top of the installation plate. A rotating plate is mounted at the top of the mechanical rotating mechanism, a placement frame is fixedly mounted at the top of the rotating plate, a plurality of groups of sample tubes are inserted into the inner side of the placement frame, a heat preservation cover is fixedly mounted in the machine shell and located on the outer side of the mounting plate, and a sealing cover is movably mounted in the machine shell and located above the heat preservation cover. An automatic sample adding mechanism is mounted on the sealing cover; the automatic sample adding mechanism comprises an electric telescopic rod fixedly installed at the rear end of the interior of the machine shell, through the design, automatic sample adding can be achieved while the manual operation frequency can be reduced, and then the sample adding efficiency and quality are improved.

Description

An automated sample dispensing device for DNA fragmentation rate detection Technical Field

[0001] This utility model relates to the field of medical device technology, specifically to an automatic sample dispensing device for DNA fragmentation rate detection. Background Technology

[0002] DNA fragmentation rate detection samples need to be pretreated before flow cytometry analysis. The current mainstream treatment method is to have a dedicated operator manually add reagent liquid to the sample tube with a pipette, and then manually put it into a vortex mixer for mixing. This process is repeated. This is not only cumbersome and inefficient, but the increased manual operation also causes fluctuations in the temperature range of the sample, affecting the processing quality.

[0003] Therefore, it is of great importance to design an automated sample loading device for DNA fragmentation rate detection to address the above-mentioned shortcomings. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention presents an automated sample loading device for DNA fragmentation rate detection. This device aims to solve the technical problems of manual sample loading in existing technologies, which are not only cumbersome and inefficient, but also cause fluctuations in the temperature storage range of the samples as manual operations increase, thus affecting the processing quality.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] An automated sample dispensing device for DNA fragmentation rate detection includes a housing, a temperature control chamber installed at the bottom of the housing, an mounting plate fixedly installed on the top of the temperature control chamber, a mechanical rotating mechanism installed on the top of the mounting plate, a rotating plate installed on the top of the mechanical rotating mechanism, a placement rack fixedly installed on the top of the rotating plate, multiple sets of sample tubes inserted into the inner side of the placement rack, a heat insulation cover fixedly installed inside the housing and outside the mounting plate, and a cap movably installed inside the housing and above the heat insulation cover, with an automated sample dispensing mechanism installed on the cap.

[0007] The automatic sample dispensing mechanism includes an electric telescopic rod fixedly installed inside the rear end of the housing. A connecting plate is fixedly installed on the drive end of the electric telescopic rod. The front end of the connecting plate is slidably connected to the cap via a lifting shaft. A sample dispensing plate is fixedly installed at the bottom end of the lifting shaft. Sample dispensing heads are installed at the bottom of the sample dispensing plate and on top of multiple sets of sample tubes. The top ends of the multiple sets of sample dispensing heads are slidably connected to the cap via movable tubes. Solenoid valves are fixedly installed at the top ends of the multiple sets of movable tubes. Diverter loops are fixedly installed on the top ends of the multiple sets of solenoid valves. A reagent kit is fixedly installed at the left end of the top of the housing. A pump is fixedly installed on the top of the reagent kit. The input end of the pump is connected to the inside of the reagent kit via a suction tube. The output end of the pump is connected to the diverter loop via a connecting hose.

[0008] As a preferred embodiment of this invention, the top of the reagent kit is threaded with a liquid filling cap, and the front of the reagent kit is provided with a visible liquid window.

[0009] As a preferred embodiment of this utility model, the temperature control box has a built-in refrigeration module and a temperature sensor. The refrigeration module includes a metal heat-conducting plate fixedly installed at the top of the inside of the temperature control box. Heat dissipation fins are fixedly installed at the bottom of the metal heat-conducting plate. A semiconductor cooling chip is embedded at the top of the heat dissipation fins. Cooling fans are fixedly installed at both ends of the bottom of the heat dissipation fins. Heat dissipation grooves are opened at the bottom of both the temperature control box and the casing.

[0010] As a preferred embodiment of this utility model, a heating module is installed on the mounting plate, and the heating module consists of silicone heating pads installed at the front and rear ends of the mounting plate.

[0011] As a preferred embodiment of this utility model, the mechanical rotation mechanism includes a motor fixedly installed at the bottom right end of the mounting plate. A heat insulation pad is provided at the connection between the motor and the mounting plate. The interior of the rotating plate is rotatably connected to the mounting plate through a rotating shaft. The drive end of the motor is connected to the rotating shaft through a synchronous belt pulley set.

[0012] As a preferred embodiment of this utility model, the rotating plate has multiple sets of heat conduction holes inside, and the bottom of the placement rack is fixedly connected to the rotating plate through multiple sets of support columns.

[0013] As a preferred embodiment of this utility model, the top of the multiple sets of sample tubes is fitted with an elastic silicone cap, and the inner side of each set of elastic silicone caps is provided with a sample insertion hole.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] In this invention, through the coordinated design of the automatic sample dispensing mechanism and sample tubes, an electric telescopic rod is used to lift the connecting plate. Under the connection of the lifting shaft, the sample dispensing plate is raised, and multiple sets of movable tubes move inside the cap. The plate continues to rise until the top of the sample dispensing plate touches the bottom of the cap, opening the cap and allowing the sample tube containing the DNA fragmentation rate detection sample to be inserted into the inner side of the placement rack. The cap is then lowered to close it. After the cap is closed, the sample dispensing plate is lowered again until multiple dispensing heads are inserted into the top of the sample tubes to complete the connection. At this point, the pump is activated, and the reagents in the reagent kit are extracted using the extraction tube and introduced into the reagent via the connecting tubing. Inside the shunt loop, the reagent volume is controlled by adjusting the opening and closing time of the solenoid valve. The sample dispensing head is inserted through the sample dispensing hole inside the elastic silicone cap, allowing the sample to be added to the sample tube. After dispensing, the elasticity of the silicone cap maintains the tightness of the inner side of the sample dispensing hole, ensuring the sample tube's seal and preventing leakage when the rack rotates. After reagent addition, the dispensing plate is raised, allowing the subsequent rotating plate to drive the rack to rotate normally. This reduces manual operation and achieves automated dispensing, thereby improving the efficiency and quality of dispensing. Attached Figure Description

[0016] Figure 1 is a schematic diagram of the overall structure of this utility model;

[0017] Figure 2 is a schematic diagram of the internal structure of the casing of this utility model;

[0018] Figure 3 is a schematic diagram of the bottom structure of the cap of this utility model;

[0019] Figure 4 is a schematic diagram of the placement rack structure of this utility model;

[0020] Figure 5 is a schematic diagram of the temperature control box structure of this utility model;

[0021] Figure 6 is a schematic diagram of the refrigeration module structure of this utility model.

[0022] In the diagram: 1. Housing; 2. Temperature control box; 201. Refrigeration module; 202. Metal heat-conducting plate; 203. Heat sink fins; 204. Semiconductor cooling chip; 205. Cooling fan; 3. Mounting plate; 301. Heating module; 302. Silicone heating element; 4. Mechanical rotation mechanism; 401. Motor; 402. Shaft; 403. Synchronous belt pulley assembly; 5. Rotating plate; 501. Heat conduction hole; 6. Placement rack; 601. Support column; 7. Sample tube; 701. Elastic silicone 702. End cap; 8. Sample insertion port; 9. Insulation cover; 10. Cap; 11. Automatic sample dispensing mechanism; 1001. Electric telescopic rod; 1002. Connecting plate; 1003. Lifting shaft; 1004. Sample dispensing plate; 1005. Sample dispensing head; 1006. Movable tube; 1007. Solenoid valve; 1008. Diverter loop; 1009. Reagent kit; 1010. Pump; 1011. Aspiration tube; 1012. Connecting hose; 1013. Liquid dispensing cap; 1014. Visual liquid window. Detailed Implementation

[0023] The technical solutions of the present utility model will be clearly and completely described below with reference to the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0024] Example: Please refer to Figures 1-6. This utility model provides a technical solution:

[0025] An automated sample dispensing device for DNA fragmentation rate detection includes a housing 1, a temperature control box 2 installed at the bottom inside the housing 1, an mounting plate 3 fixedly installed on the top of the temperature control box 2, a mechanical rotation mechanism 4 installed on the top of the mounting plate 3, a rotating plate 5 installed on the top of the mechanical rotation mechanism 4, a placement rack 6 fixedly installed on the top of the rotating plate 5, multiple sets of sample tubes 7 inserted into the inner side of the placement rack 6, a heat preservation cover 8 fixedly installed inside the housing 1 and outside the mounting plate 3, a cover 9 movably installed inside the housing 1 and above the heat preservation cover 8, and an automated sample dispensing mechanism 10 installed on the cover 9.

[0026] First, in this embodiment, the specific structure of the automatic sampling mechanism 10 is as follows:

[0027] The automatic sample dispensing mechanism 10 includes an electric telescopic rod 1001 fixedly installed inside the rear end of the housing 1. A connecting plate 1002 is fixedly installed on the drive end of the electric telescopic rod 1001. The front end of the connecting plate 1002 is slidably connected to the cap 9 via a lifting shaft 1003. A sample dispensing plate 1004 is fixedly installed at the bottom end of the lifting shaft 1003. A sample dispensing head 1005 is installed at the bottom of the sample dispensing plate 1004 and on top of multiple sample tubes 7. The top ends of the multiple sample dispensing heads 1005 are slidably connected to the cap 9 via a movable tube 1006. The device is connected to a series of movable tubes 1006, each with a solenoid valve 1007 fixedly mounted at its top. A shunt ring tube 1008 is fixedly mounted on the top of each solenoid valve 1007. A reagent kit 1009 is fixedly mounted on the left side of the top of the housing 1. A pump 1010 is fixedly mounted on the top of the reagent kit 1009. The input end of the pump 1010 is connected to the interior of the reagent kit 1009 via a suction tube 1011, and the output end of the pump 1010 is connected to the shunt ring tube 1008 via a connecting hose 1012. The device is first operated using an electric telescopic mechanism. Rod 1001 pushes the connecting plate 1002 upwards, and under the connection of the lifting shaft 1003, it drives the sample application plate 1004 to rise. Multiple sets of movable tubes 1006 then move inside the cap 9 until the top of the sample application plate 1004 touches the bottom of the cap 9. The cap continues to rise, opening the cap 9 and inserting the sample tube 7 containing the DNA fragmentation rate detection sample into the inside of the placement rack 6. The cap 9 is then lowered to close it. After the cap 9 is closed, the sample application plate 1004 is lowered again until multiple sets of sample application heads 1005 are inserted into the inside of the sample tube 7. Once the top connection is complete, the pump 1010 is started, and the reagent in the reagent kit 1009 is extracted using the extraction tube 1011. The reagent is then introduced into the shunt loop tube 1008 through the connecting tube 1012. The reagent volume is controlled by controlling the opening and closing time of the solenoid valve 1007. After the reagent is added, the sample plate 1004 is raised so that the subsequent rotating plate 5 can drive the placement rack 6 to rotate normally. This reduces the number of manual operations and realizes automatic sample addition, thereby improving the efficiency and quality of sample addition.

[0028] Furthermore, the top of the reagent kit 1009 is threaded with a liquid filling cap 1013, and the front of the reagent kit 1009 is provided with a viewing window 1014. Reagents are added to the inside of the reagent kit 1009 through the liquid filling cap 1013, and the remaining amount can be easily observed and replenished in time through the viewing window 1014.

[0029] Then, the temperature control box 2 has a built-in cooling module 201 and a temperature sensor. The cooling module 201 includes a metal heat-conducting plate 202 fixedly installed at the top of the inside of the temperature control box 2. A heat dissipation fin 203 is fixedly installed at the bottom of the metal heat-conducting plate 202. A semiconductor cooling chip 204 is embedded at the top of the heat dissipation fin 203. A cooling fan 205 is fixedly installed at both ends of the bottom of the heat dissipation fin 203. The bottom of the temperature control box 2 and the casing 1 are both provided with heat dissipation slots. The semiconductor cooling chip 204 is used for cooling and the metal heat-conducting plate 202 is used for rapid heat conduction to improve the cooling efficiency, thereby meeting the temperature requirements of the operating environment. The heat dissipation fin 203 and the cooling fan 205 dissipate heat from the hot end of the semiconductor cooling chip 204. Finally, the heat is discharged through the heat dissipation slots, which together ensure the normal operation of the cooling module 201.

[0030] Furthermore, a heating module 301 is installed on the mounting plate 3. The heating module 301 consists of silicone heating pads 302 installed at both ends of the mounting plate 3. At the same time, the temperature can be monitored by the temperature sensor built into the temperature control box 2. While the cooling module 201 meets the low temperature requirements, the heating module 301 can also provide heating, thereby improving the applicability of the device.

[0031] The mechanical rotation mechanism 4 includes a motor 401 fixedly installed at the bottom right end of the mounting plate 3. A heat insulation pad is provided at the connection between the motor 401 and the mounting plate 3. The interior of the rotating plate 5 is rotatably connected to the mounting plate 3 through a rotating shaft 402. The drive end of the motor 401 is connected to the rotating shaft 402 through a synchronous pulley set 403. After the reagent is added, the motor 401 is started and driven by the synchronous pulley set 403 to rotate the rotating shaft 402. The rotating shaft 402 drives the rotating plate 5 to rotate, thereby mixing the sample and reagent inside the sample tube 7.

[0032] Secondly, the rotating plate 5 has multiple sets of heat conduction holes 501 inside. The bottom of the placement rack 6 is fixedly connected to the rotating plate 5 through multiple sets of support columns 601. The multiple sets of heat conduction holes 501 facilitate heat conduction, which makes it easier for the cooling module 201 and the heating module 301 to control the temperature. The support columns 601 elevate the placement rack 6 to prevent the placement rack 6 from blocking the heat conduction holes 501.

[0033] Finally, elastic silicone caps 701 are installed at the top of multiple sample tubes 7, and each set of elastic silicone caps 701 has a sample insertion hole 702 on its inner side. The sample insertion head 1005 is inserted through the sample insertion hole 702 inside the elastic silicone cap 701, so that the sample tube 7 can be sampled. After the sample is added, the elasticity of the elastic silicone cap 701 can maintain the tightness of the inner side of the sample insertion hole 702, thereby ensuring the sealing of the sample tube 7 and preventing the sample from leaking out when the rack 6 rotates.

[0034] In this embodiment, the specific implementation scenario is as follows: First, the connecting plate 1002 is lifted using the electric telescopic rod 1001. Under the connection of the lifting shaft 1003, the sample loading plate 1004 is raised, and multiple sets of movable tubes 1006 move inside the cap 9. The process continues until the top of the sample loading plate 1004 touches the bottom of the cap 9, at which point it is further lifted, causing the cap 9 to open. The sample tube 7 containing the DNA fragmentation rate detection sample is then inserted into the inside of the placement rack 6. The cap 9 is then lowered to close it. After the cap 9 is closed... Lower the sample plate 1004 until multiple sample tips 1005 are inserted into the top of the sample tube 7 and connected. Then, start the pump 1010 and use the suction tube 1011 to extract the reagent from the reagent kit 1009. Connect the reagent to the shunt loop 1008 via the connecting tubing 1012. Control the reagent volume by controlling the opening and closing time of the solenoid valve 1007. Insert the sample tip 1005 through the sample insertion hole 702 inside the elastic silicone cap 701, thus allowing the sample tube 7 to be filled. The sample is added inside the tube 7. After the sample is added, the elastic silicone cap 701 maintains the tightness of the inside of the sample insertion hole 702, thus ensuring the sealing of the sample tube 7 and preventing leakage of the sample inside the sample tube 7 when the rack 6 rotates. After the reagent is added, the sample plate 1004 is raised so that the subsequent rotating plate 5 can drive the rack 6 to rotate normally. The temperature sensor built into the temperature control box 2 can monitor the temperature. The cooling module 201 meets the low temperature requirement, and the heating module 301 can also provide heating to meet the temperature requirements of the operating environment. After the reagent is added, the start motor 401 drives the rotating shaft 402 to rotate under the transmission of the synchronous pulley group 403. The rotating shaft 402 drives the rotating plate 5 to rotate, so that the sample and reagent inside the sample tube 7 are mixed evenly. The whole operation process is simple and convenient. This utility model can reduce the number of manual operations and realize automatic sample addition, thereby improving the efficiency and quality of sample addition.

[0035] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An automated sample dispensing device for DNA fragmentation rate detection, comprising a housing (1), characterized in that: A temperature control box (2) is installed at the bottom inside the casing (1). A mounting plate (3) is fixedly installed on the top of the temperature control box (2). A mechanical rotating mechanism (4) is installed on the top of the mounting plate (3). A rotating plate (5) is installed on the top of the mechanical rotating mechanism (4). A placement rack (6) is fixedly installed on the top of the rotating plate (5). Multiple sets of sample tubes (7) are inserted into the inner side of the placement rack (6). The casing (1) is located inside and outside the mounting plate (3). A heat insulation cover (8) is fixedly installed inside the housing (1) and above the heat insulation cover (8). An automatic sample dispensing mechanism (10) is installed on the cover (9). The automatic sample dispensing mechanism (10) includes an electric telescopic rod (1001) fixedly installed at the rear end inside the housing (1). A connecting plate (1002) is fixedly installed on the drive end of the electric telescopic rod (1001). The front end of the connecting plate (1002) is connected to a lifting shaft (1002). 3) Sliding connection with the cap (9), the bottom end of the lifting shaft (1003) is fixedly installed with a sample feeding plate (1004), the bottom of the sample feeding plate (1004) and the top of the multiple sample tubes (7) are each equipped with a sample feeding head (1005), the top of the multiple sample feeding heads (1005) are all slidably connected to the cap (9) through a movable tube (1006), the top of the multiple movable tubes (1006) are all fixedly installed with a solenoid valve (1007), the multiple solenoid valves A diversion ring tube (1008) is fixedly installed on the top of (1007), and a reagent kit (1009) is fixedly installed on the left end of the top of the housing (1). A pump (1010) is fixedly installed on the top of the reagent kit (1009). The input end of the pump (1010) is connected to the inside of the reagent kit (1009) through a liquid extraction tube (1011), and the output end of the pump (1010) is connected to the diversion ring tube (1008) through a connecting hose (1012).

2. The automatic sample dispensing device for DNA fragmentation rate detection according to claim 1, characterized in that: The reagent kit (1009) has a liquid filling cap (1013) threaded to its top, and a visible liquid window (1014) is provided on the front of the reagent kit (1009).

3. The automatic sample dispensing device for DNA fragmentation rate detection according to claim 1, characterized in that: The temperature control box (2) has a built-in refrigeration module (201) and a temperature sensor. The refrigeration module (201) includes a metal heat-conducting plate (202) fixedly installed at the top of the inside of the temperature control box (2). A heat dissipation fin (203) is fixedly installed at the bottom of the metal heat-conducting plate (202). A semiconductor cooling chip (204) is embedded at the top of the heat dissipation fin (203). A cooling fan (205) is fixedly installed at both ends of the bottom of the heat dissipation fin (203). The bottom of the temperature control box (2) and the casing (1) are both provided with heat dissipation grooves.

4. The automatic sample dispensing device for DNA fragmentation rate detection according to claim 1, characterized in that: A heating module (301) is installed on the mounting plate (3), and the heating module (301) consists of silicone heating pads (302) installed at the front and rear ends of the mounting plate (3).

5. The automatic sample dispensing device for DNA fragmentation rate detection according to claim 1, characterized in that: The mechanical rotating mechanism (4) includes a motor (401) fixedly installed at the bottom right end of the mounting plate (3). A heat insulation pad is provided at the connection between the motor (401) and the mounting plate (3). The interior of the rotating plate (5) is rotatably connected to the mounting plate (3) through a rotating shaft (402). The drive end of the motor (401) is connected to the rotating shaft (402) through a synchronous pulley set (403).

6. The automatic sample dispensing device for DNA fragmentation rate detection according to claim 1, characterized in that: The rotating plate (5) has multiple sets of heat conduction holes (501) inside, and the bottom of the placement rack (6) is fixedly connected to the rotating plate (5) through multiple sets of support columns (601).

7. The automatic sample dispensing device for DNA fragmentation rate detection according to claim 1, characterized in that: The top of each of the multiple sets of sample tubes (7) is fitted with an elastic silicone cap (701), and the inner side of each set of elastic silicone caps (701) is provided with a sample insertion hole (702).

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