Centrifugal positioning perfusion device and perfusion system

Abstract

The utility model provides a centrifugal positioning perfusion device which comprises a sample loading module connected with a rotation driving device. The centrifugal far end is provided with a positioning space; the liquid conveying module comprises a guide part which rotates along with the sample loading module and a liquid supply part which rotates relative to the sample loading module and is positioned; the guide part is provided with a first inlet and a second inlet, and the first inlet is communicated with the second inlet; the liquid supply part is provided with a liquid supply channel, the liquid supply channel is communicated and matched with the first inlet, and the second inlet is communicated to the positioning space position through a conveying pipeline; the infusion module is provided with a liquid outlet; and the positioning space position and the liquid outlet are connected through a conveying pipeline to form flow channel communication. By means of the positioning spaces with the multiple ends arranged separately, the guide part rotating along with relative rotation movement and the liquid supply part positioned along with relative rotation movement are arranged in the liquid conveying module, and mixed solution input application of all external conveying modules can be stably connected.

Description

Technical Field

[0001] This utility model relates to the field of cell sample perfusion technology, specifically to a centrifugation-positioned perfusion device and perfusion system. Background Technology

[0002] Currently, in ultra-low temperature vitrification experiments of cell samples, after loading cells or tissues into the cryoprotectant and before immersing them in liquid nitrogen for vitrification, it is necessary to perform a perfusion infiltration operation with a cryoprotectant. This involves using a high concentration of cryoprotectant to replace the tissue fluid inside and outside the cells, in order to prevent the formation of ice crystals in biological tissues, cells, or organs during cooling, which could damage the cell lipid membrane and cytoskeleton structure and affect cell viability.

[0003] In routine perfusion infiltration experiments, the infiltration of cells, biological tissues, or organs with cryoprotectants, as well as the reverse washing during thawing, are primarily performed manually by the experimenter. The typical procedure involves the experimenter manually preparing the appropriate cryoprotectant based on the sample to be frozen and adding it to each sample individually. After equilibrating the samples with different concentrations of cryoprotectant, the cryoprotectant carrier containing the frozen samples is then immersed in liquid nitrogen for vitrification. This entire process is quite cumbersome, taking 50-100 minutes to process 10 samples, which is insufficient for the cryopreservation of large quantities of biological tissues.

[0004] In the prior art, patent document CN118511872A describes a sample loading device and perfusion system for perfusion applications, which employs a processing device for perfusion and wetting via centrifugal rotation. However, this sample loading device is structured with independent centrifugal ends at both ends, resulting in low working efficiency; moreover, its overall horizontal configuration leads to a relatively low centrifugal perfusion and wetting effect. Furthermore, for cell sample recovery, a dedicated sample recovery module is required for independent application, and the structure necessitates the combination of multiple independent devices, lacking overall integration. Utility Model Content

[0005] To overcome the shortcomings of the prior art, this utility model provides a centrifugal positioning irrigation device and irrigation system.

[0006] A centrifugal perfusion device includes a sample-carrying module connected to a rotation drive for centrifugal rotation; a positioning space is provided at the distal end of the centrifuge; an infusion module is located at the rotation axis of the sample-carrying module, including a guide portion that rotates with the sample-carrying module and a supply portion positioned relative to the rotation of the sample-carrying module; a first inlet is provided on the guide portion, and a second inlet is provided on the outer periphery of the guide portion, the first inlet and the second inlet being connected; a supply channel is provided on the supply portion, the supply channel being connected and cooperating with the first inlet, and the second inlet being connected to the positioning space via a delivery pipe; the infusion module is provided with an outlet; the positioning space and the outlet are connected by a delivery pipe to form a flow channel.

[0007] Furthermore, the infusion module also includes a drain section positioned relative to the sample carrier module by rotational movement; the outlet includes a first outlet on the outer periphery of the guide section and a second outlet corresponding to the drain section, the first outlet and the second outlet are connected, a drain channel is provided on the drain section, and the drain channel is connected and cooperates with the second outlet; the first outlet is connected to the positioning space position through a delivery pipe.

[0008] Furthermore, the sample carrying module includes a sample carrying disk arranged in a horizontal direction, and the infusion module is located at the rotation axis of the sample carrying disk; multiple positioning spaces are provided, and each positioning space is located at the same radial position around the sample carrying disk.

[0009] Furthermore, the sample carrier has a recessed working space on its upper side; the outer periphery of the recessed working space has a working end face that is inclined from top to bottom and surrounds the inner side; the positioning space is cylindrical and extends inclined from the rotation axis position away from the sample carrier module and from the working end face position toward the lower and outer side.

[0010] Furthermore, the centrifugal end of each positioning space is narrowed into a cone shape; each positioning space is provided with a positioning opening on one side of the working end face, and a plug is installed on the positioning opening by interference fit; a two-end connecting pipe with a connecting port is connected to the positioning space through the plug, one end of the connecting pipe is connected to the second inlet position through a delivery pipe, and the other end of the connecting pipe is connected to the liquid outlet position.

[0011] Furthermore, the drain section is provided with a waste liquid collection chamber for waste liquid collection and buffering as a drain channel, and a drain port is provided on the lower outer periphery of the waste liquid collection chamber.

[0012] Furthermore, it also includes a carrier component, which has an inner cavity for loading cell samples and is detachably fitted to the positioning space; the second inlet is connected to the inner cavity of the carrier component through a delivery pipe; the inner cavity of the carrier component and the outlet are connected to form a flow channel through the delivery pipe.

[0013] Furthermore, the bearing assembly includes a sample carrier cylinder that is generally cylindrical, with a mounting end at one end and a closed positioning end at the other end. The inner cavity is disposed within the sample carrier cylinder. An inlet pipe and a outlet pipe are connected to the mounting end. One end of the inlet pipe extends into the positioning end, and the extension distance of the inlet pipe is greater than the extension distance of the outlet pipe. The inlet pipe and the outlet pipe are connected to the second inlet and outlet through a delivery pipeline.

[0014] Furthermore, the positioning end of the sample carrier is tapered; the mounting end of the sample carrier is detachably fitted with a sealing plug, and the inlet pipe and outlet pipe are fixedly connected to the sealing plug.

[0015] The perfusion system, which uses the perfusion device described above, further includes: a plurality of delivery modules for receiving reagent solutions, wherein the outlets of each delivery module for each reagent solution converge and are connected to the supply channel of the supply unit; a temperature control module having a heat exchange connection with the positioning space; and a data processing module, which stores temperature curves adapted to the corresponding cell samples and delivery curves corresponding to each delivery module to form a perfusion control scheme; the data processing module controls the working status of the temperature control module and each delivery module.

[0016] The beneficial effects of this utility model are as follows:

[0017] The perfusion device utilizes a centrifugally driven sample loading module, in conjunction with an infusion module, to effectively perfuse and infiltrate cell samples placed on the sample loading module. Furthermore, by designing multiple positioning spaces at various ends, along with guide sections and supply sections within the infusion module that rotate relative to each other and are positioned relative to each other, it can stably connect to external delivery modules for mixed solution input. During the mixed solution input process, the solution can be input to multiple positions at each positioning space via centrifugal motion, further realizing multi-end centrifugal perfusion and infiltration operations. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the structural assembly of the irrigation device of this utility model;

[0019] Figure 2 This is a cross-sectional structural diagram of the irrigation device of this utility model;

[0020] Figure 3 yes Figure 2 A magnified view of part A.

[0021] Explanation of reference numerals in the attached figures:

[0022] Sample carrying module 1; rotation drive device 11; positioning space 12; sample carrying tray 13; working space 131; working end face 132; support bracket 14.

[0023] Supporting component 2; inner cavity 21, sample carrier 22, mounting end 23, positioning end 24, sealing plug 25, liquid inlet pipe 26, liquid outlet pipe 27.

[0024] Infusion module 3; inlet 31, first inlet 311, second inlet 312, outlet 32, first outlet 321, second outlet 322, guide 33, support plate 331, supply section 34, supply channel 341, first supply port 342, second supply port 343, drain section 35, outer baffle 351, waste liquid collection chamber 352, drain port 353. Detailed Implementation

[0025] To make the technical solution, purpose and advantages of this utility model clearer, the following explanation is given in conjunction with the accompanying drawings and embodiments.

[0026] For perfusion applications of cell samples, this utility model provides a perfusion system, which includes:

[0027] The perfusion device includes a positioning space 12 for placing cell samples, and the perfusion device is connected to a temperature control module.

[0028] Several delivery modules are used to connect reagent solutions; each delivery module is connected to the perfusion device through a delivery pipe, so that each delivery module is connected to the positioning space 12.

[0029] The data processing module stores delivery curves adapted to each delivery module and records temperature curves for corresponding cell samples. The delivery curves and temperature curves are combined to form a perfusion control scheme.

[0030] According to the perfusion control scheme, the data processing module adjusts the feed rate of each delivery module to ensure that the feed rate of each reagent solution is consistent with the corresponding delivery curve, so that the reagent solutions are mixed at the outlets of each delivery module to form a mixed solution; the data processing module adjusts and controls the temperature control module to ensure that the real-time temperature at the positioning space 12 in the perfusion device is consistent with the temperature curve; the mixed solution enters the temperature-controlled perfusion device through the flow channel to infiltrate the cell samples positioned in the positioning space 12.

[0031] Example 1

[0032] This embodiment describes an application example of a perfusion device that uses centrifugal rotation to position cell samples and connects them to various reagent solutions for mixing and transport, thereby achieving perfusion and infiltration of cell samples.

[0033] like Figures 1 to 2 As shown, the perfusion device includes a sample-carrying module 1, which is connected to a rotation drive device 11 for driving it to make centrifugal rotation.

[0034] The sample carrying module 1 includes a frustum-shaped sample tray 13, which is arranged horizontally and has a recessed upper side forming a working space 131. The outer periphery of the recessed working space 131 has a working end face that slopes downwards and surrounds the inner side. Positioning spaces 12 are radially evenly distributed at the same horizontal position on the working end face, and each positioning space 12 is located at the centrifugal distal end of the sample carrying module 1. The temperature control module has a heat exchange connection with the positioning space 12.

[0035] It also includes an infusion module 3, which is provided with an inlet 31 and an outlet 32. The infusion module 3 is cylindrical and vertically positioned at the axial position of the sample tray 13 to ensure that the infusion module 3 can equally control the supply and discharge of liquid to each positioning space 12.

[0036] Specifically, the infusion module 3 includes a guide portion 33 that rotates with the sample carrier module 1, a supply portion 34 that is positioned relative to the sample carrier module 1, and a drain portion 35 that is positioned relative to the sample carrier module 1. The guide portion 33 is located in the working space 131, the supply portion 34 is located at the upper end of the guide portion 33, and the drain portion 35 is located at the lower end of the guide portion 33.

[0037] The guide section 33 rotates around its axis with the sample loading module 1. Under the positioning setting of the liquid supply section 34, the external mixed solution can be positioned and transported from the liquid supply section 34. Then, the stably input mixed solution is centrifugally transported to the positioning space at the end of the centrifuge as the guide section 33 rotates. In the above-mentioned transport module application, the outlets of each transport module of each reagent solution converge and connect to the inlet 31 of the liquid infusion module 3. The inlet 31 includes a first inlet 311 provided at the rotation axis of the guide section 33 and a second inlet 312 provided on the outer periphery of the guide section 33. The first inlet 311 and the second inlet 312 are connected. The liquid supply section 34 is provided with a liquid supply channel 341, which is connected and cooperates with the first inlet 311. The second inlet 312 can be connected to the positioning space 12 through a transport pipe, guiding the mixed solution into the positioning space 12.

[0038] As the irrigation process proceeds, waste liquid is generated and needs to be discharged. The guide section 33 discharges the waste liquid through the outlet 32 ​​to the drain section 35. The waste liquid recovery device then connects to the fixed drain section 35 via a pipeline to recover the waste mixed solution, etc. The outlet includes a first outlet 321 on the outer periphery of the guide section 33 and a second outlet 322 corresponding to the drain section 35. The first outlet 321 and the second outlet 322 are connected. The drain section 35 is provided with a drain channel, which is connected to an external waste liquid collection position and communicates with the second outlet 322. The first outlet 321 can be connected to the positioning space 12 through a conveying pipeline to guide the waste liquid in the positioning space 12 out.

[0039] To facilitate installation, the second inlet 312 and the first outlet 321 are both located within the working space 131. The second inlet 312 is located above the first outlet 321. Two sets of the second inlet 312 and the first outlet 321 are symmetrically arranged along the guide portion 33. Each second inlet 312 and the first outlet 321 extends horizontally to facilitate the positioning and connection of the conveying pipeline into a cylindrical structure.

[0040] Its application principle and overall working process are as follows:

[0041] The outlets of the externally installed delivery modules that receive multiple reagent solutions converge and are connected to the liquid supply section 34 and the liquid supply channel 341 via connecting pipes, thereby achieving liquid supply communication between each delivery module and the liquid inlet 31 of the delivery module 3.

[0042] By driving the rotation drive device 11 to drive the sample loading module 1 to perform centrifugal rotation, the reagent mixture solution, etc., flows through the liquid supply channel 341 and the liquid inlet 31 to the positioning space 12 under the supply pressure and centrifugal force. In this embodiment, cell sample perfusion can be performed directly at the positioning space 12. The positioning space 12 is provided with two-end connection interfaces for positioning and connecting the delivery pipe, so that the delivery pipe connects the positioning space 12 and the liquid inlet 31 and the liquid outlet 32 ​​of the infusion module 3, which meets the application requirements for the input and output of the mixed solution.

[0043] The working process and application steps of the irrigation device are as follows:

[0044] A) The cell sample is carried in a carrier fluid and fed into the positioning space 12 through the inlet 31 of the infusion module 3. The sample-carrying module 1 is centrifuged and rotated. The cell sample fed into the positioning space 12 will be continuously adhered and fixed to one end of the positioning space 12 under the centrifugal force.

[0045] B) Discharge the carrier liquid input into the sample loading module 1 through the outlet 32.

[0046] C) Mix the reagent solutions to form a mixed solution, and input the mixed solution into the sample loading module 1 through the inlet 31 of the infusion module 3.

[0047] D) Obtain a perfusion control scheme with temperature and delivery curves. According to the perfusion control scheme, adjust the real-time temperature of the positioning space 12 in the sample loading module 1 to be consistent with the temperature curve, and make the feed amount of each reagent solution consistent with the corresponding delivery curve, so that the mixed solution is input into the positioning space 12 to perfuse and infiltrate the cell sample.

[0048] E) After the soaking is completed, the mixed solution is discharged through the infusion module 3, and the cell sample is continuously attached and fixed to one end of the positioning space 12 under the centrifugal force.

[0049] F) After the mixed solution is discharged, the carrier liquid is introduced into the positioning space 12; then the centrifugal rotation of the sample loading module 1 is stopped, and the cell sample is discharged from the outlet 32 ​​along with the carrier liquid.

[0050] G) Extract the discharged cell samples and transfer them to a vitrified refrigerated container for cold storage.

[0051] H) Repeat steps A to G to complete the large-scale perfusion infiltration treatment of cell samples.

[0052] Specifically, in order to effectively connect with external connecting pipes and ensure stable centrifugal operation, the liquid supply part 34 is disposed on the upper side of the guide part 33, and the liquid discharge part 35 is disposed on the lower side of the guide part 33. The liquid supply part 34 is configured to have a first liquid supply port 342 for positioning and connecting with external connecting pipes on its outer peripheral side, and a second liquid supply port 343 is disposed on the lower center of the liquid supply part 34 corresponding to the rotation axis. The first liquid supply port 342 and the second liquid supply port 343 are connected to form the liquid supply channel 341.

[0053] In this embodiment, the perfusion application of cell samples is directly set in the positioning space 12. As a preferred structural form, the positioning space 12 is cylindrical and extends downward at an angle away from the rotation axis of the sample-carrying module 1. Multiple positioning spaces 12 are provided, each located at the same radial position around the sample-carrying tray 13. The centrifugation end of each positioning space 12 is narrowed into a cone shape for positioning biological cell sample centrifugation perfusion. Each positioning space 12 has a positioning opening on the side corresponding to the rotation axis of the sample-carrying module 1. A plug is installed on the positioning opening by interference fit, and a connecting pipe with a connecting port is positioned on the plug. This allows the delivery pipeline to connect the inlet 31 and outlet 32 ​​of the infusion module 3 to the positioning space 12 through the connection pipe.

[0054] Example 2:

[0055] This embodiment is proposed based on the structural principle of Embodiment 1 above.

[0056] The perfusion device application in this embodiment also includes a support component 2, which has an inner cavity for loading cell samples and is detachably mounted to the positioning space 12. In this embodiment, the perfusion application of cell samples involves centrifuging and positioning the biological cell samples within the inner cavity of the support component 2. The support component 2 also has correspondingly provided end-to-end connecting interfaces for positioning and connecting the delivery pipe, thereby enabling the flow channel communication between the inner cavity of the support component 2 and the inlet 31 and outlet 32 ​​of the infusion module 3.

[0057] As a preferred structural embodiment, the bearing component 2 includes a sample carrier 22 that is generally cylindrical, with a mounting end 23 at one end and a positioning end 24 closed at the other end, and the inner cavity is disposed inside the sample carrier 22.

[0058] Given that each of the positioning spaces 12 is located at the same radial position around the sample tray 13, and each positioning space 12 is cylindrical and extends downwards at an incline away from the rotation axis of the sample carrier module 1, the outer diameter of the sample carrier cylinder 22 in the bearing assembly 2 is adapted to the inner diameter of the positioning space 12, so that its positioning end 24 is inserted into the bottom of the positioning space 12 to complete the assembly and positioning. The positioning end 24 of the sample carrier cylinder 22 is tapered, and there is a certain installation gap between the bottom of the positioning end 24 of the assembled and positioned sample carrier cylinder 22 and the bottom of the positioning space 12.

[0059] The inlet pipe 26 and outlet pipe 27 are connected to the mounting end 23. One end of the inlet pipe 26 extends towards the positioning end 24, and the extension distance of the inlet pipe 26 is greater than that of the outlet pipe 27. The inlet pipe 26 and outlet pipe 27 are connected to the inlet port 31 and outlet port 32 through a delivery pipe. By extending one end of the inlet pipe 26 towards the positioning end 24, the flow of the input mixed solution through the inlet pipe 26 to the cell sample fixing position at the bottom of the positioning end 24 of the sample carrier 22 can be more effectively organized. At the same time, by setting the different extension distances of the inlet pipe 26 and outlet pipe 27, the solution can be more easily discharged into the outlet pipe 27 during the drainage process.

[0060] The mounting end 23 of the bearing component 2 is provided with a sealing plug 25 (as described above for the installation of a plug). The liquid inlet pipe 26 and the liquid outlet pipe 27 are fixedly connected to the sealing plug 25, which simply and reliably meets the installation and application requirements of the liquid inlet pipe 26 and the liquid outlet pipe 27 relative to the bearing component 2 (sample cylinder 22).

[0061] Based on the configuration of the carrier component 2, which is detachably mounted to the positioning space 12 in the sample carrier module 1, the carrier component 2 can serve as a container for positioning cell samples during perfusion and also as a storage container for cell samples after perfusion. The carrier component 2, containing cell samples, can be directly refrigerated after perfusion, thus meeting the refrigeration requirements for cell samples after perfusion. For refrigerated storage, the sealing plug 25 connecting the inlet pipe 26 and the outlet pipe 27 can be replaced with another sealing plug 25 with a fully enclosed end face, thus meeting the requirements for sealed storage of cell samples after perfusion.

[0062] In contrast to the case in Example 1 where cell samples are discharged from the outlet 32 ​​along with the carrier liquid and collected externally, in Example 2, the cell samples are perfused and then left on the carrier component 2.

[0063] In this case, in the perfusion device structure of this embodiment, for the outlet 32 ​​position, the drainage channel of the drainage section 35 can be configured to form a waste liquid collection chamber 352 for waste liquid accumulation and collection. Specifically, the second outlet 322 is located on the lower outer periphery of the guide section 33 and is located below the sample carrying module 1; the waste liquid collection chamber 352 is arranged around the second outlet 322. The sample carrying device of this utility model includes a bottom support bracket 14, the rotation drive device 11 is used as a drive motor, the rotation drive device 11 is arranged on the lower side of the support bracket 14 and its drive shaft extends from the upper side of the support bracket 14, and the drive shaft of the rotation drive device 11 is positioned and connected to the central axis position of the guide section 33; the lower outer periphery of the guide section 33 is horizontally extended with a support plate 331 corresponding to the lower side of the sample carrying module 1 to support the rotation of the sample carrying module 1 (sample tray 13).

[0064] The drainage section 35 includes an outer baffle 351 disposed on the upper side of the support bracket 14 (around the second outlet 322 of the guide section 33). The inner side of the outer baffle 351 encloses and forms the drainage channel (waste liquid collection chamber 352), and a drainage port 353 is provided on the lower outer periphery of the outer baffle 351. During the entire cycle of centrifugal perfusion, the drainage port 353 is closed, and the mixed solution generated is discharged from the second outlet 322 and temporarily stored in the waste liquid collection chamber 352. After the centrifugal perfusion is completed, the user can proceed to collect cell samples while simultaneously opening the drainage port 353 to collect the waste liquid in a specific waste liquid collection container; this completes the waste liquid collection and cell sample collection in one go, improving work efficiency.

[0065] The above description is only a preferred embodiment of the present utility model. For those skilled in the art, modifications can still be made to the embodiments without departing from the implementation principle of the present utility model, and the corresponding modifications should also be considered within the protection scope of the present utility model.

Claims

1. A centrifugal positioning irrigation device, characterized in that, include: The sample carrier module is connected to a rotary drive device for driving it to rotate centrifugally. Its centrifugal end is equipped with a positioning space; An infusion module is positioned at the rotation axis of a sample carrier module. It includes a guide portion that rotates with the sample carrier module and a supply portion that is positioned relative to the rotation of the sample carrier module. The guide portion has a first inlet and a second inlet on its outer periphery, and the first inlet and the second inlet are connected. The supply portion has a supply channel that is connected to and cooperates with the first inlet. The second inlet is connected to the positioning space position through a delivery pipe. The infusion module is provided with an outlet; the positioning space and the outlet are connected by a delivery pipe to form a flow channel.

2. The irrigation device as described in claim 1, characterized in that, The infusion module further includes a drain section positioned relative to the sample carrier module by rotational movement; the outlet includes a first outlet on the outer periphery of the guide section and a second outlet corresponding to the drain section, the first outlet and the second outlet are connected, a drain channel is provided on the drain section, and the drain channel is connected and cooperates with the second outlet; the first outlet is connected to the positioning space position through a delivery pipe.

3. The irrigation device as described in claim 1, characterized in that, The sample carrying module includes a sample carrying disk arranged in a horizontal direction, and the infusion module is located at the rotation axis of the sample carrying disk; multiple positioning spaces are provided, and each positioning space is located at the same radial position around the sample carrying disk.

4. The irrigation device as described in claim 3, characterized in that, The sample carrier tray has a recessed upper side to form a working space; the outer periphery of the recessed working space has a working end face that is inclined from top to bottom and surrounds the inner side; the positioning space is cylindrical and extends inclined from the rotation axis position away from the sample carrier module to the lower and outer side of the working end face position.

5. The irrigation device as described in claim 4, characterized in that, Each of the positioning spaces has a narrowed, tapered end; each of the positioning spaces has a positioning opening on one side of the working end face, and a plug is installed on the positioning opening by interference fit; the plug is positioned to connect two connecting pipes with connecting ports into the positioning space, one end of the connecting pipe is connected to the second inlet position through a delivery pipe, and the other end of the connecting pipe is connected to the outlet position.

6. The irrigation device as described in claim 2, characterized in that, The drain section is provided with a waste liquid collection chamber for waste liquid collection and buffering as a drain channel, and a drain port is provided on the lower outer periphery of the waste liquid collection chamber.

7. The irrigation device according to any one of claims 1 to 6, characterized in that, It also includes a carrier component, which has an inner cavity for loading cell samples and is detachably fitted to the positioning space; the second inlet is connected to the inner cavity of the carrier component through a delivery pipe; the inner cavity of the carrier component and the outlet are connected to form a flow channel through the delivery pipe.

8. The irrigation device as described in claim 7, characterized in that, The supporting component includes a sample carrier cylinder that is generally cylindrical, with a mounting end at one end and a closed positioning end at the other end. The inner cavity is located inside the sample carrier cylinder. An inlet pipe and a outlet pipe are connected to the mounting end. One end of the inlet pipe extends into the positioning end and the extension distance of the inlet pipe is greater than that of the outlet pipe. The inlet pipe and the outlet pipe are connected to the second inlet and outlet through a delivery pipe.

9. The irrigation device as described in claim 8, characterized in that, The positioning end of the sample carrier is conical; the mounting end of the sample carrier is detachably fitted with a sealing plug, and the inlet pipe and outlet pipe are fixedly connected to the sealing plug.

10. An irrigation system, characterized in that, The irrigation apparatus as described in any one of claims 1 to 9 further includes: Several delivery modules for receiving reagent solutions are provided, and the outlets of each delivery module for each reagent solution converge and connect to the supply channel of the supply unit. The temperature control module has a heat exchange connection with the positioning space; The data processing module stores temperature curves adapted to the corresponding cell samples and delivery curves corresponding to each delivery module, forming a perfusion control scheme; the data processing module controls the working status of the temperature control module and each delivery module.

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