Nucleic acid extraction temperature control module and gene sequencing pretreatment system

Abstract

The utility model provides a nucleic acid extraction temperature control module and a gene sequencing pretreatment system, and relates to the technical field of gene sequencing, and the nucleic acid extraction temperature control module comprises a plate seat, a temperature control plate, a heating block bottom assembly, a reagent heating block and an upper cover plate which are sequentially arranged from bottom to top, a plurality of heating block connecting grooves are formed in the heating block bottom assembly side by side, the reagent heating block is detachably connected with the single heating block connecting groove and electrically connected with the temperature control plate, the upper cover plate is buckled with the plate seat, an opening is formed in the upper cover plate, and the reagent heating block is arranged in the opening. The opening enables the reagent heating block connected to the heating block bottom assembly to be located on the upper side of the upper cover plate in a protruding mode. According to the technical scheme, the technical problems that in the prior art, a temperature control unit is large in size and the position of the heating block is inflexible to change are solved.

Description

Technical Field

[0001] This utility model relates to the field of gene sequencing technology, and in particular to a nucleic acid extraction temperature control module and a gene sequencing pretreatment system. Background Technology

[0002] In the fields of genetic engineering and protein engineering, nucleic acid molecules are the main research objects. The extraction process of nucleic acid requires the use of heating modules. Generally, heating modules are designed as separate units with fixed positions that cannot be changed. Due to structural limitations, separate reagent strips or reagent holders cause problems such as inaccurate positioning and difficulty in placement. Summary of the Invention

[0003] This invention provides a nucleic acid extraction temperature control module and a gene sequencing pretreatment system, which solves the technical problems of large temperature control unit size and inflexible heating block position adjustment in the prior art.

[0004] On one hand, this utility model provides a nucleic acid extraction temperature control module, including a plate base, a temperature control plate, a heating block bottom assembly, a reagent heating block, and an upper cover plate arranged sequentially from bottom to top. The heating block bottom assembly has multiple heating block connection slots arranged side by side. The reagent heating block is detachably connected to a single heating block connection slot and electrically connected to the temperature control plate. The upper cover plate is fastened to the plate base. The upper cover plate has an opening, which allows the reagent heating block connected to the heating block bottom assembly to protrude and be positioned on the upper side of the upper cover plate.

[0005] Optionally, the reagent heating block is strip-shaped, and each reagent heating block is provided with a row of heating holes.

[0006] Optionally, the heating block bottom assembly is configured as multiple, and the multiple heating block bottom assemblies are arranged side by side.

[0007] Optionally, a heating strip is provided at the bottom of the reagent heating block, the heating strip is electrically connected to the temperature control board, and a temperature protection switch is attached to the heating strip.

[0008] Optionally, the reagent heating block is provided with a heat insulation component on its outer periphery.

[0009] Optionally, it also includes a heat insulation cover plate, which engages with the heating block connection groove where no reagent heating block is provided.

[0010] Optionally, a positioning component is provided on the upper surface of the cover plate corresponding to the outer side of the reagent holder.

[0011] Optionally, the positioning component is configured as an elastic positioning element whose positioning surface is an elastic button and / or a positioning corner block located at the four corners of the upper surface of the upper cover plate.

[0012] Optionally, a positioning plate is also included, which is disposed on the upper side of the plate base for fixing the bottom assembly of the heating block.

[0013] On the other hand, this utility model provides a gene sequencing pretreatment system, characterized in that it includes a nucleic acid extraction temperature control module as described above.

[0014] To make the features and advantages of this application more apparent and understandable, some embodiments are described below in detail with reference to the accompanying drawings. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0016] Figure 1 This is a schematic diagram of the nucleic acid extraction process provided in the embodiments of this application;

[0017] Figure 2 This is a schematic diagram of the gene sequencing preprocessing system in the embodiments of this application;

[0018] Figure 3 This is a schematic diagram of the structure of the electronic control system in the embodiments of this application;

[0019] Figure 4 This is an exploded view of a nucleic acid extraction temperature control module according to an embodiment of the present invention;

[0020] Figure 5 This is a schematic diagram illustrating the usage status of a nucleic acid extraction temperature control module according to an embodiment of the present invention;

[0021] Figure 6 This is a schematic diagram of the structure of a nucleic acid extraction temperature control module according to another embodiment of the present invention;

[0022] Figure 7 This is a schematic diagram of the usage status of a nucleic acid extraction temperature control module according to another embodiment of the present invention;

[0023] Figure 8 This is a schematic diagram of the usage status of a nucleic acid extraction temperature control module according to another embodiment of the present invention;

[0024] Figure 9 This is a schematic diagram of the positioning component of a nucleic acid extraction temperature control module according to an embodiment of the present invention.

[0025] In the diagram: 10. Nucleic acid extraction system; 20. Library construction system; 30. Quality control system; 40. Electrical control system; 41. Central control module; 42. Nucleic acid extraction system control module; 43. Library construction system control module; 44. Quality control system control module; 45. Auxiliary component control module; 50. Host computer; 60. 96-well plate; 70. Reagent strip tray; 71. Elution well; 72. Lysis well; 80. Large-capacity reagent kit; 81. Long strip well; 420. Temperature control module; 421. Nucleic acid extraction platform motion control module; 422. Magnetic rod / magnetic rod sleeve motion control module; 430. Library construction pipetting assembly motion control module; 431. Library construction manipulator assembly motion control module; 432. Shaking and heating assembly control module; 433. Reagent container library construction instrument display structure; 440. Quality control manipulator. 441. Hand component motion control module; 442. Conveying mechanism motion control module; 443. Quality control pipetting component motion control module; 444. Quantitative analyzer control module; 450. Camera control module; 451. Puncture component motion control module; 452. Ultraviolet lamp and other light source control module; 453. Air cleaning control module; 4201. Plate base; 4202. Heating block bottom component; 4203. Reagent heating block; 4204. Top cover plate; 4205. Temperature control plate; 4206. Elastic positioning component; 4207. Heat insulation cover plate; 4208. Heat insulation block; 4209. High positioning corner block; 42010. Bearing mounting bracket; 42031. Inclined chamfer; 42032. Long strip hole heating block; 42041. Opening; 42061. Elastic button; 42062. Elastic component; 42091. Low positioning corner block. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be described in detail below. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other implementation methods obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0027] It should be noted that when a component is referred to as "fixed to" or "set on" another component, it can be directly on the other component or may have an intervening component present. When a component is referred to as "connected to" another component, it can be directly connected to the other component or may have an intervening component present.

[0028] Figure 1 This is a schematic diagram of the nucleic acid extraction process. Figure 1As shown in the embodiments of this application, nucleic acid extraction is a technique for separating nucleic acids from biological samples using physical, chemical, biological methods, or a combination of these methods. It is mainly used to separate nucleic acid molecules (such as DNA or RNA) from biological samples for subsequent experimental analysis, such as sequencing analysis.

[0029] Taking chemical separation as an example, cell lysis buffer is used to lyse sample cells, releasing nucleic acid molecules. However, these nucleic acid molecules, along with impurities such as proteins, coexist in the lysis product solution. To obtain pure nucleic acid molecules, further purification of the lysis product is necessary. Using magnetic bead purification as an example, magnetic beads are added to the lysis product. The nucleic acid molecules released from the sample cells are specifically adsorbed onto the surface of the magnetic beads, while impurities such as proteins remain in the lysis product. After a certain reaction time, under the influence of a magnetic field, the magnetic beads adsorbed with nucleic acid molecules separate from the solution. The magnetic beads are then washed with a washing solution to remove impurities such as proteins. This washing process can be repeated multiple times to ensure thorough cleaning and recover the pure magnetic beads adsorbed with nucleic acid molecules. Finally, elution with an elution buffer de-adsorbs the nucleic acid molecules from the magnetic beads. After a certain reaction time, the magnetic beads are separated from the nucleic acid molecules again under the influence of a magnetic field. After removing the magnetic beads, only pure nucleic acid molecules remain in the nucleic acid extraction product.

[0030] Furthermore, this application provides quality control for nucleic acid extraction products. The detection (or quantitative analysis) of the concentration of nucleic acid molecules (such as DNA or RNA) in the nucleic acid extraction product solution is crucial. Subsequent library construction typically requires a certain total amount of nucleic acid; if the actual amount of nucleic acid input exceeds this requirement, it will affect the amplification efficiency during library construction. Therefore, it is necessary to detect the nucleic acid concentration in the nucleic acid extraction product before library construction. After detecting the nucleic acid concentration, the required total volume of the nucleic acid extraction product can be determined based on the concentration and the total amount of nucleic acid required for subsequent library construction. Moreover, if the detected nucleic acid concentration is too high, the required total volume of the nucleic acid extraction product will be lower than the minimum pipetting volume of the pipette and affect pipetting accuracy. Therefore, it is necessary to dilute the nucleic acid extraction product appropriately with a diluent (e.g., pure water) before pipetting. Common concentration detection methods include spectrophotometry, fluorescent dye detection, microfluidic analysis, or capillary gel electrophoresis.

[0031] Furthermore, this application provides library construction. In next-generation sequencing (NGS) technology, nucleic acid molecules (such as DNA or RNA) extracted from biological samples need to be broken down by physical or enzymatic methods, such as by ultrasound. After breaking, nucleic acid fragments are formed. First, enzymes are used to fill in the ends of the nucleic acid fragments, and then specific enzymes are used to connect the ends of the fragments to specific DNA sequences (usually, this specific DNA sequence is also called a linker). Finally, the nucleic acid fragments are formed, which are referred to in the industry as libraries.

[0032] To save sequencing costs, multiple biological samples are typically sequenced simultaneously in a sequencer. To differentiate the sequencing results of different samples, when preparing libraries for different samples, a DNA sequence (usually containing 6-8 bases) is included in the adapters to identify the sample's origin. This DNA sequence can also be called a sample tag (or index, barcode). Understandably, each sample's library adapter contains its own unique sample tag.

[0033] Furthermore, this application provides quality control for library construction products. The quality of the constructed library is crucial to the data quality of subsequent gene sequencing output. Therefore, it is necessary to detect and control the quality of the library construction products before sequencing. Typically, library quality detection includes at least one of library length detection, library concentration detection, and library contaminant detection. Among these, library concentration detection (or quantitative concentration analysis) can detect the library concentration. When the detected library concentration is too high, the total volume of the library construction products required for subsequent sequencing will be lower than the minimum pipetting volume of the pipetting device, affecting pipetting accuracy. Therefore, it is necessary to add diluent (e.g., pure water) to the library construction products for appropriate dilution before pipetting. Furthermore, differences in library concentrations and sequencing data output among multiple biological samples lead to variations in the total volume of library construction products required for subsequent sequencing, ultimately affecting the balance of sequencing data output from multiple biological samples. Therefore, it is necessary to dilute some of the library construction products of biological samples with diluent (e.g., pure water) before pipetting to reduce the difference in the total volume of library construction products among multiple biological samples. Common concentration detection methods include spectrophotometry, fluorescent dye detection, microfluidic analysis, or capillary gel electrophoresis.

[0034] It should be noted that the concentration detection of library construction product quality control and nucleic acid extraction product quality control can be performed using the same method or different methods, and this application does not limit this.

[0035] Figure 2This is a schematic diagram of the gene sequencing preprocessing system structure in this application embodiment. Further, the gene sequencing preprocessing system (or sequencing preprocessor) provided in this application embodiment includes: a nucleic acid extraction system 10, a library construction system 20, a quality control system 30, an electronic control system 40, and a host computer 50. Wherein:

[0036] Nucleic acid extraction system 10, configured to controllably release nucleic acid molecules (such as DNA or RNA) from biological samples;

[0037] Library construction system 20, configured to controllably prepare nucleic acid molecules (such as DNA or RNA) into libraries that can be used for sequencing.

[0038] The quality control system 30 is configured to controllably detect the concentration of nucleic acid molecules in the nucleic acid extraction product and adjust the concentration of nucleic acid molecules to a predetermined concentration range based on the detection results, and / or controllably detect the concentration of the library in the library construction product and adjust the concentration of the library to a predetermined concentration range based on the detection results.

[0039] The electrical control system 40 is configured to control the operation and function of the nucleic acid extraction system 10, the library construction system 20, and the quality control system 30 according to the instructions of the host computer 50.

[0040] The host computer 50 is configured to control the operation and function of the nucleic acid extraction system 10, the library construction system 20, and the quality control system 30 by sending control commands to the electronic control system 40.

[0041] like Figure 3 As shown, the electronic control system 40 may include, but is not limited to: nucleic acid extraction system control module 42, library construction system control module 43, quality control system control module 44, and auxiliary component control module 45. Taking magnetic bead purification as an example, the nucleic acid extraction system control module 42 includes at least: a temperature control module 420, a nucleic acid extraction platform motion control module 421, and a magnetic rod / magnetic rod sleeve motion control module 422; the quality control system control module 44 includes at least: a quality control operator component motion control module 440, a conveyor mechanism motion control module 441, a quality control pipetting component motion control module 442, and a quantitative analyzer control module 443; the library construction system control module 43 includes at least: a library construction pipetting component motion control module 430, a library construction operator component motion control module 431, a shaking and heating component control module 432, and a library construction instrument display structure 433; the auxiliary component control module 45 includes at least: a camera control module 450, a puncture component motion control module 451, an ultraviolet lamp and other light source control module 452, and an air cleaning control module 453. Each module is described in detail below.

[0042] The temperature control module 420 can control the heating device to provide suitable reaction temperatures and conditions for various chemical reactions (such as cell lysis, washing, and elution) occurring on the nucleic acid extraction platform. For example, the heating device can be a heating element or a TEC (thermal condenser) module. Furthermore, to achieve better temperature control, the temperature control module 420 can also implement closed-loop control based on the temperature collected by the temperature sensor.

[0043] The nucleic acid extraction platform motion control module 421 and the magnetic rod / magnetic rod sleeve motion control module 422 work together to achieve relative movement between the nucleic acid extraction platform and the magnetic rod / magnetic rod sleeve, completing each step of the nucleic acid extraction process. Taking the movement of the nucleic acid extraction platform in a two-dimensional plane as an example, the nucleic acid extraction platform motion control module 421 can drive the X-direction motor and the Y-direction motor respectively through the driver, thereby causing the nucleic acid extraction platform to move in the X and Y directions (the X and Y directions are perpendicular to each other on the horizontal plane). The magnetic rod / magnetic rod sleeve motion control module 422 can drive the Z-direction motor respectively through the driver, causing the magnetic rod and the magnetic rod sleeve to move in the Z direction (the Z direction is perpendicular to the horizontal plane formed by the X and Y directions).

[0044] The motion control module 440 of the quality control manipulator assembly can drive the X-axis motor, Y-axis motor and Z-axis motor respectively through the control driver, thereby driving the quality control manipulator assembly to move in the X, Y and Z directions.

[0045] The motion control module 441 of the conveyor mechanism can drive the X-direction motor to move the conveyor mechanism in the X direction by controlling the driver.

[0046] The quality control pipetting assembly motion control module 442 can drive the X-axis motor, Y-axis motor and Z-axis motor respectively by controlling the driver, thereby driving the quality control pipetting assembly to move in the X, Y and Z directions.

[0047] The quantitative analyzer control module 443 can control the quantitative analyzer to detect the concentration of at least one of the nucleic acid extraction products and library construction products, and output the detection results.

[0048] The library-built pipetting assembly motion control module 430 can drive the X-axis motor, Y-axis motor and Z-axis motor respectively through the control driver, thereby driving the library-built pipetting assembly to move in the X, Y and Z directions.

[0049] The motion control module 431 for the library construction manipulator component can drive the X-axis motor, Y-axis motor and Z-axis motor respectively through the control driver, thereby driving the library construction manipulator component to move in the X, Y and Z directions.

[0050] The oscillation heating component control module 432 can control the oscillation and heating devices to provide suitable reaction temperatures and conditions for various reactions (such as washing and elution reactions) in magnetic bead purification. For example, the heating device can be a heating element or a TEC (thermal refrigeration unit) module. Furthermore, to achieve better temperature control, the oscillation heating component control module 432 can also implement closed-loop control based on the temperature collected by the temperature sensor.

[0051] The library builder display structure 433 can provide a suitable refrigeration temperature for the library construction reagent containers by controlling heating and cooling devices. For example, the heating device can be a TEC (thermal refrigeration unit) module, and the cooling device can be a fan. Furthermore, to achieve better temperature control, the library builder display structure 433 can also implement closed-loop control based on the temperature collected by the temperature sensor.

[0052] The reagent-adding area library builder display structure 434 can provide a suitable refrigeration temperature in the reagent-adding area by controlling heating and cooling equipment. For example, the heating equipment can be a TEC (thermal refrigeration unit) module, and the cooling equipment can be a fan. Furthermore, to achieve better temperature control, the reagent-adding area library builder display structure 434 can also implement closed-loop control based on the temperature collected by the temperature sensor.

[0053] The camera control module 440 can control the X-axis motor, Y-axis motor, and Z-axis motor to move in the X, Y, and Z directions, and to take pictures of the consumables (e.g., extraction operation containers, library construction reagent containers) in the sequencing pretreatment instrument after reaching the target position, and report the images to the host computer. For example, a QR code carrying consumable information is set on the consumable. By controlling the camera to take pictures of the QR code on the consumable, the host computer can identify the consumable information based on the QR code. In addition, the camera control module can also control the camera to take pictures of relevant containers (e.g., library construction reagent containers, construction reaction containers, purification containers placed on the shaking and heating assembly, pipette tip containers for pipetting pumps placed in the pipette tip storage area, etc.) and report the images to the host computer 50, so that the host computer 50 can verify the placement and orientation of the relevant containers and the relevant consumable information before the pretreatment experiment begins, and determine whether each container is placed correctly and whether the consumables are used correctly.

[0054] The puncture component motion control module 441 can drive the X-direction motor, Y-direction motor and Z-direction motor to move in the X, Y and Z directions respectively by controlling the driver.

[0055] The air cleaning control module 443 provides clean air to the inside of the instrument housing and the nucleic acid extraction system by controlling the air intake mechanism (e.g., a fan) and the exhaust mechanism (e.g., a blower).

[0056] In addition to the various control modules mentioned above, the electronic control system 40 also needs to collect information reflecting the instrument's operating status in real time through various sensors, and report it to the host computer 50 through the central control module 41, so that the host computer 50 can detect the instrument's working status and ensure the instrument's stable operation.

[0057] Various sensors may include, but are not limited to: temperature sensors for collecting the temperature of key components inside the sequencer, and sensors for detecting the position status of relevant components (including but not limited to limit position detection and zero position detection).

[0058] The status detection of the nucleic acid extraction platform may include, but is not limited to, the status detection of the nucleic acid extraction platform, magnetic rod / magnetic rod sleeve assembly, quality control manipulator assembly, transfer mechanism, quality control pipetting assembly, library construction manipulator assembly, library construction pipetting assembly, nucleic acid extraction system compartment door, and instrument compartment door.

[0059] Temperature sensors can collect the temperature of nucleic acid extraction platforms, oscillating heating components, reagent addition areas, etc., and the central control module can further form closed-loop control based on the temperature collected by the temperature sensors.

[0060] In addition, temperature sensors can collect the temperature of the heat sink in the TEC module. The central control module can further determine whether the TEC module is operating normally based on the temperature of the heat sink, and perform alarms, stop operation, and other controls in case of abnormal operation.

[0061] The central control module 41 can also receive input power, convert the input power into the working power required by each control module as needed, and output it.

[0062] The temperature control module 420 provides suitable reaction temperatures and conditions for various chemical reactions occurring on the nucleic acid extraction platform (such as cell lysis, washing, and elution reactions). To enable heating at different locations, this invention provides a nucleic acid extraction temperature control module.

[0063] Figures 4 to 8 The diagram illustrates the structure of the nucleic acid extraction temperature control module in an embodiment of this utility model.

[0064] This utility model provides a nucleic acid extraction temperature control module, such as... Figure 4As shown, the assembly includes, from bottom to top, a plate base 4201, a temperature control plate 4205, a heating block bottom assembly 4202, a reagent heating block 4203, and an upper cover plate 4204. Multiple heating block connecting slots are arranged side-by-side on the heating block bottom assembly 4202. The reagent heating block 4203 is detachably connected to a single heating block connecting slot and electrically connected to the temperature control plate. The upper cover plate 4204 is fastened to the plate base 4201. The upper cover plate 4204 has an opening 42041, which allows the reagent heating block 4203 connected to the heating block bottom assembly 4202 to protrude from the upper side of the upper cover plate 4204.

[0065] Specifically, in this solution, the bottom assembly 4202 of the heating block is mounted on the plate base 4201, and the heating block connecting slots (not shown in the figure) are arranged side by side on the bottom assembly 4202. The reagent heating block 4203 is detachably connected to the heating block connecting slots. A heating belt is provided at the bottom of the reagent heating block 4203, and the heating belt is electrically connected to the temperature control board 4205 so that the temperature control board 4205 controls the heating belt to heat the reagent heating block 4203. In actual use, the reagent heating block 4203 is installed in the corresponding heating block connecting slot according to the required heating position. The control board 4205 controls the heating of the reagent heating block 4203 at the required heating position, thereby heating the reagent placed inside the reagent heating block 4203. The reagent heating block 4203 protrudes from the upper cover plate 4204. During heating, the reagent tubes (such as elution tubes, lysis tubes, etc.) are located in the reagent tray (such as 96-well plate 60, reagent strip tray 70, large-volume reagent kit 80, etc.). The reagent tray is placed directly on the upper cover plate 4204, and the reagent tubes in the reagent tray are aligned with the corresponding reagent heating block 4203 and placed inside the reagent heating block 4203, where they are heated by the reagent heating block 4203. By pre-setting the reagent block heating slots that may be used, the actual heating blocks are configured according to the specific heating position, and the heating position is flexibly adjustable to meet the heating needs of different heating positions by the temperature control module.

[0066] As an optional implementation, the reagent heating block 4203 is strip-shaped, and each reagent heating block is provided with a row of heating holes.

[0067] Specifically, the reagent heating block 4203 is configured according to the structure of the heating target. For example, for a 96-well plate 60 used in nucleic acid extraction, each reagent heating block 4203 is configured with 8 heating holes.

[0068] As an optional implementation, multiple heating block bottom components 4202 are provided, and the multiple heating block bottom components 4202 are arranged side by side.

[0069] Specifically, depending on the arrangement of the required heating positions, the bottom component 4202 of the heating block can be configured as a whole or in parts to correspond to the arrangement of the required heating positions.

[0070] On the other hand, this utility model provides a gene sequencing pretreatment system, characterized in that it includes any of the above-mentioned nucleic acid extraction temperature control modules 420.

[0071] Based on the above description, this application also provides the following embodiments:

[0072] Example 1

[0073] 96-hole plate heating module:

[0074] like Figure 4 , Figure 5 As shown, the 96-well plate heating module can simultaneously control the temperature of two 96-well plates 60. Each 96-well plate 60 includes 1 to 12 columns of wells, divided into a first group: columns 1-6 and a second group: columns 7-12. Taking the first group as an example, the module can simultaneously heat columns 1, 2, 5, and 6, and the positions of these columns can be interchanged. Alternatively, any column can be selected for heating. The temperature control module 420 is equipped with reagent heating blocks 4203 corresponding to the positions that need to be heated, and the positions that do not need to be heated are provided with heat insulation covers 4207. The heat insulation covers are heat insulation structures that are fastened to the connecting grooves of heating blocks without reagent heating blocks. Figure 4 The illustrated embodiment only shows the reagent heating block 4202 for heating wells in columns 1 and 5. The positions of the modules corresponding to wells in columns 2 and 6 are shown as heat insulation covers 4207. The positions of the reagent heating blocks 4203 corresponding to wells in columns 1, 2, 5, and 6 in the illustration are interchangeable. In actual operation, each group can support simultaneous heating of up to 4 wells (such as wells 1, 2, 5, and 6 in the first group). The upper surface of the cover plate 4204 of the 96-well plate heating module is a mirror-finished stainless steel cover, which facilitates wiping and reflects more ultraviolet light to enhance sterilization. An internal temperature control plate 4205 controls the temperature of the reagent heating blocks 4203. Heat insulation components are provided on the outer periphery of the reagent heating blocks 4203, and the heating block connecting groove is composed of heat insulation blocks. The reagent heating block 4203 is installed in the heating block connecting groove. A heat insulation component is provided at its bottom to prevent heat transfer from the heating block. A temperature sensor (not shown in the figure) and a heating belt (not shown in the figure) are installed on the reagent heating block 4203. The temperature control board 4205 heats the actual heating block 4203 through the heating belt. A temperature protection switch (not shown in the figure) is attached to the heating belt; if the temperature control fails, it can automatically disconnect the heating, protecting the component. The plate base 4201 is a base structure responsible for supporting the entire mechanism. A bearing mounting bracket 42010 is installed at the bottom of the plate base 4201 to provide end support for the temperature control module, preventing deformation of the plate base 4201 due to long-term use, which could lead to poor contact consistency with the bottom of the 96-well plate 60. Figure 9As shown, the upper part of the cover plate 4204 is a positioning component, including an elastic positioning element 4206. The positioning surface of the elastic positioning element 4206 is an elastic button 42061, and the inside is an elastic component 42062, which can pre-press the 96-well plate 60 to prevent displacement during movement. The special reagent heating block 4203 uses a blackening process on its surface. After blackening, the surface will be smooth, which makes it easier for the 96-well plate 60 to be placed on the reagent heating block 4203. In addition, the heating holes on the reagent heating block 4203 have a certain depth, which can wrap the reagent tube and heat up faster.

[0075] The advantages of this 96-hole plate heating module are:

[0076] 1. The reagent heating blocks are interchangeable, and each group supports heating of up to 4 rows of wells;

[0077] 2. The positioning components with pre-pressure are compatible with 96-hole plates of different sizes, making them more adaptable;

[0078] 3. The modular design of the combination of temperature control board 4205, bottom component of heating block 4202 and reagent heating block 4203 can make higher utilization of module space and higher integration.

[0079] 4. Adding heat insulation components around the bottom of the heating block can improve the heating effect and enhance the sealing of the module.

[0080] Example 2

[0081] Single reagent strip heating module:

[0082] like Figure 6 , Figure 7As shown, the single reagent strip heating module can simultaneously control the temperature of 32 single reagent strips, divided into 4 groups of 8 reagent strips each. As shown in Figure 7, each reagent strip has an elution well 71 and a lysis well 72 at both ends. The numbers at the end of the reagent strip tray 70 correspond to the 8 reagent strips in each group. Each group of reagent strips corresponds to 2 heating blocks on the temperature control module 420, which can heat the elution well 71 and the lysis well 72 respectively. The elution well 71 and the lysis well 72 are of different sizes. In order to better adapt to them, the temperature control module 420 has an inverted conical inclined chamfer 42031 on the upper part of the heating hole on the reagent heating block 4203. This design makes it easier for the lower end of the reagent strip to be inserted into the heating hole. The 32 reagent strips can be placed into the reagent strip tray 70 first. Then, they are placed together on the temperature control module 420 for easier operation. The upper cover plate 4204 of the temperature control module 420 has four reagent tray positioning corner blocks 4209 on its upper side. These are a set of front-low, rear-high positioning corner blocks, including high positioning corner block 4209 and low positioning corner block 42091. These blocks not only position the reagent strip tray 70 but also facilitate placement. Specifically, the supporting part of the reagent strip tray 70 has a forward-protruding boss design. When the reagent strip tray 70 is lifted, its bottom is suspended, and the boss provides support. When the reagent strip tray 70 is placed on the module, its bottom rests on the upper cover plate 4204, at which point the boss is no longer under pressure. This design has two advantages: 1. The bottom contact of the reagent strip tray 70 makes heating more efficient. 2. It ensures better consistency of the bottoms of the 32 reagent strips, increasing the consistency of the extraction effect. In particular, the reagent strip tray 70 has an ergonomic handle design on its protrusion, with handles at both the top and bottom for easier handling. The upper surface of the reagent strip tray 70 is engraved with numbers 1-32 (one set of 8 numbers is shown on the left end in the illustration, while the numbers of the other three sets are obscured by the reagent strip in the illustration), which allows users to more clearly observe the position of the reagent strip. The reagent strip tray 70 has a hollow design in the front, back and middle parts to reduce the weight of the reagent tray. Other components are similar to the heating module of the 96-well plate.

[0083] Advantages of this single-reagent-strip heating module:

[0084] 1. The single reagent tray + reagent strip tray structure makes it more convenient to use;

[0085] 2. The positioning component's front-low, back-high design simplifies the placement of the reagent strip tray;

[0086] 3. The protruding handle design of the reagent strip tray is ergonomic and makes operation more convenient.

[0087] Example 3

[0088] Large-capacity reagent kit heating module:

[0089] like Figure 8 As shown, the large-volume reagent kit heating module can simultaneously control the temperature of four large-volume reagent kits. Each kit can hold eight samples, which are placed in the elongated well positions 81. The installation method of the heating module is similar to that of a single reagent strip (the installation method of the reagent tray is different). The lysis well is heated by the elongated well position 81 on the reagent kit, making the heating more efficient. The positioning component is similar to that of a 96-well plate, with four positioning corner blocks 4209 for corner positioning and front and rear elastic positioning parts 4206 for pre-compression positioning. One reagent kit integrates eight samples, which is suitable for large-volume applications.

[0090] The present invention provides a temperature control module 420 for adapting to different reagent holders. It is based on a modular design integrating a temperature control board 4205, a heating block bottom component 4202, and a reagent heating block 4203. The heating blocks can be freely interchanged, and heating of different heating positions can be achieved in a small volume space. It is very flexible and can be adapted to the heating needs of different manufacturers.

[0091] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0092] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0093] The above are merely specific embodiments of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. A nucleic acid extraction temperature control module, characterized in that, The device includes, from bottom to top, a plate base, a temperature control plate, a bottom heating block assembly, a reagent heating block, and a top cover. The bottom heating block assembly has multiple heating block connection slots arranged side by side. The reagent heating block is detachably connected to a single heating block connection slot and electrically connected to the temperature control plate. The top cover is fastened to the plate base and has an opening that allows the reagent heating block connected to the bottom heating block assembly to protrude from the upper side of the top cover.

2. The nucleic acid extraction temperature control module according to claim 1, characterized in that, The reagent heating block is strip-shaped, and each reagent heating block is provided with a row of heating holes.

3. The nucleic acid extraction temperature control module according to claim 2, characterized in that, The heating block bottom component is configured as multiple components, and the multiple heating block bottom components are arranged side by side.

4. The nucleic acid extraction temperature control module according to claim 1, characterized in that, A heating strip is provided at the bottom of the reagent heating block. The heating strip is electrically connected to the temperature control board, and a temperature protection switch is attached to the heating strip.

5. The nucleic acid extraction temperature control module according to claim 1, characterized in that, The reagent heating block is provided with a heat insulation component on its outer periphery.

6. The nucleic acid extraction temperature control module according to claim 1, characterized in that, It also includes a heat insulation cover plate, which engages with the heating block connection groove where no reagent heating block is provided.

7. The nucleic acid extraction temperature control module according to claim 1, characterized in that, A positioning component is provided on the upper surface of the cover plate corresponding to the outer side of the reagent holder.

8. The nucleic acid extraction temperature control module according to claim 7, characterized in that, The positioning component is configured as an elastic positioning element whose positioning surface is an elastic button and / or a positioning corner block located at the four corners of the upper surface of the upper cover plate.

9. The nucleic acid extraction temperature control module according to claim 1, characterized in that, It also includes a positioning plate, which is disposed on the upper side of the plate base and is used to fix the bottom assembly of the heating block.

10. A gene sequencing preprocessing system, characterized in that, Includes the nucleic acid extraction temperature control module as described in any one of claims 1-9.

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