Perfusion equipment for stem cell culture

Abstract

The utility model discloses perfusion equipment for stem cell culture, which relates to the technical field of perfusion equipment and comprises a base, a mounting block is arranged on one side of the top surface of the base, a control panel is hinged on one side of the top surface of the mounting block, a culture tank is inserted in the middle of the top surface of the base, and a sealing cover is screwed on the top surface of the culture tank in a threaded manner. A plurality of liquid inlets are formed in the top surface of the sealing cover along the axial side at equal intervals, the liquid inlets are connected with pipelines through connecting assemblies, and culture solution bags are arranged at the tail ends of the pipelines. According to the stem cell culture device, the base is stably matched with the culture tank, so that the culture tank is convenient to mount and fix, the operation stability of the device is improved, and an efficient stem cell culture environment can be realized; through linkage cooperation of the control panel and the speed regulator, the flow rate of the culture solution can be accurately adjusted, and the control precision of the culture process is improved; finally, the problem that pipelines of existing culture equipment are inconvenient to disassemble and assemble is solved, and the stem cell culture effect and efficiency are improved.

Description

Technical Field

[0001] This utility model relates to the field of perfusion equipment technology, and in particular to a perfusion device for stem cell culture. Background Technology

[0002] A perfusion device for stem cell culture is a specialized device used to maintain the growth environment of stem cells. It typically consists of a culture tank, a culture medium delivery system, and a control system. In the existing technology, the culture medium delivery system mostly uses a pipe connection to transport the culture medium from the culture medium bag to the culture tank. The pipe connections in the existing equipment mostly use a simple plug-in connection method. Although the plug-in method is simple to operate, the connection is not stable and the pipes are prone to falling off due to vibration or external force, which affects the normal delivery of the culture medium. Therefore, it is necessary to improve the above-mentioned problems. Utility Model Content

[0003] The purpose of this invention is to address the shortcomings of existing technologies by proposing a perfusion device for stem cell culture.

[0004] To achieve the above objectives, the present invention adopts the following technical solution: a perfusion device for stem cell culture, comprising a base, an installation block on one side of the top surface of the base, a control panel hinged to one side of the top surface of the installation block, a culture tank inserted into the center of the top surface of the base, a sealing cap threaded onto the top surface of the culture tank, a plurality of liquid inlets equidistantly arranged along the axial side of the top surface of the sealing cap, a pipe connected to the liquid inlet via a connecting assembly, and a culture medium bag at the end of the pipe.

[0005] Preferably, the culture medium bag has an injection tube on one side of its top surface, a cap is screwed onto the injection tube, the cap has multiple anti-slip patterns evenly spaced on it, and the culture tank has an outlet on one side of its bottom.

[0006] Preferably, a plurality of mounting seats are provided at equal intervals on the opposite surface of the mounting block and the culture tank, and a speed regulator is installed on one of the mounting seats, with a pipe clamped and passing through the speed regulator.

[0007] Preferably, the pipe is made of plastic flexible tubing, a drive motor is provided in the center of the top surface of the sealing cap, a rotating shaft is coaxially fixed to the output shaft of the drive motor, and a stirring blade is fixed to the bottom end of the rotating shaft.

[0008] Preferably, the connecting assembly includes an inner sleeve, on which a plurality of locking steel balls are equidistantly arranged along the axial distance. A sliding sleeve is fitted over the inner sleeve, with a lifting spring at the bottom end of the sliding sleeve. A limiting block is provided on the top surface of the inner sleeve. The lower half of the sliding sleeve abuts against the locking steel balls, and the upper half of the sliding sleeve has a spring-loaded groove.

[0009] Preferably, the inlet is provided with an internal thread, the bottom end of the inner sleeve is fixedly provided with an external threaded cylinder, a partition is provided between the external threaded cylinder and the inner sleeve, an outer sleeve extends from the partition at the outer wall of the inlet, and a waterproof rubber is provided between the outer sleeve and the inlet.

[0010] Compared with existing technologies, the beneficial effects of this invention are as follows: The stable fit between the base and the culture tank facilitates the installation and fixation of the culture tank, improving the stability of equipment operation and thus enabling a highly efficient stem cell culture environment. The linkage between the control panel and the speed controller facilitates precise adjustment of the culture medium flow rate, improving the control accuracy of the culture process and enabling efficient delivery and distribution of the culture medium. The threaded fit between the sealing cap and the inlet facilitates quick sealing and opening, improving operational convenience and enabling rapid replacement and replenishment of the culture medium. The coaxial fit between the stirring blades and the drive motor facilitates uniform stirring of the culture medium, improving the mixing effect and enabling efficient stem cell culture. Finally, it solves the problem of inconvenient disassembly and installation of pipes in existing culture equipment, improving the effect and efficiency of stem cell culture. Attached Figure Description

[0011] The accompanying drawings, which are included to provide a further understanding of the present invention and form part of this application, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0012] Figure 1 This is a first-view schematic diagram of the overall structure proposed in this utility model;

[0013] Figure 2 This is a second-view schematic diagram of the overall structure proposed in this utility model;

[0014] Figure 3 This is a schematic cross-sectional view of the culture tank proposed in this utility model;

[0015] Figure 4 This is a schematic cross-sectional view of the connecting component proposed in this utility model.

[0016] The numbers in the diagram are: 1. Base; 2. Control panel; 3. Culture tank; 4. Sealing cap; 5. Liquid inlet; 6. Pipeline; 7. Culture medium bag; 8. Speed ​​controller; 9. Stirring blades; 10. Inner sleeve; 11. Locking steel ball; 12. Sliding sleeve. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0018] Example: See Figure 1-4 This utility model discloses a perfusion device for stem cell culture, comprising a base 1, a mounting block on one side of the top surface of the base 1, a control panel 2 hinged to one side of the top surface of the mounting block, a culture tank 3 inserted into the center of the top surface of the base 1, a sealing cap 4 threaded onto the top surface of the culture tank 3, and multiple liquid inlets 5 equidistantly arranged along the axial side of the top surface of the sealing cap 4. The liquid inlets 5 are connected to pipes 6 via connecting components, and a culture medium bag 7 is located at the end of the pipes 6. The insertion and connection between the base 1 and the culture tank 3 facilitates the installation and fixation of the culture tank 3, improving the stability of the equipment operation; the threaded connection between the sealing cap 4 and the liquid inlets 5 facilitates quick sealing and opening, improving the convenience of operation, and enabling rapid replacement and replenishment of the culture medium. A liquid injection tube is provided on one side of the top surface of the culture tank 7, and a bottle cap is screwed onto the injection tube. Multiple anti-slip grooves are evenly spaced on the bottle cap. A liquid outlet is provided on one side of the bottom of the culture tank 3. The addition and sealing of the culture medium is facilitated by the screwing of the injection tube of the culture medium bag 7 and the bottle cap, which improves the convenience of operation. The liquid outlet design at the bottom of the culture tank 3 facilitates the discharge of waste liquid and improves the efficiency of the culture process. Multiple mounting seats are evenly spaced on the opposite surface of the mounting block and the culture tank 3. A speed controller 8 is installed on one of the mounting seats. A pipe 6 is clamped through the speed controller 8. The clamping and cooperation between the speed controller 8 and the pipe 6 facilitates the precise adjustment of the flow rate of the culture medium, improves the control accuracy of the culture process, and enables efficient delivery and distribution of the culture medium.

[0019] In this invention, the pipe 6 is made of plastic flexible tubing. A drive motor is located in the center of the top surface of the sealing cap 4. A rotating shaft is coaxially fixed to the output shaft of the drive motor, and a stirring blade 9 is fixed to the bottom end of the rotating shaft. The coaxial cooperation between the stirring blade 9 and the drive motor facilitates uniform stirring of the culture medium, improving the mixing effect of the culture medium and enabling efficient culture of stem cells. The connecting component includes an inner sleeve 10, on which multiple locking steel balls 11 are equidistantly arranged along the axial distance. A sliding sleeve 12 is fitted over the inner sleeve 10, with a lifting spring at the bottom end of the sliding sleeve 12. A limiting block is located on the top surface of the inner sleeve 10. The lower half of the sliding sleeve 12 abuts against the locking steel balls 11, and the upper half of the sliding sleeve 12 has a spring-loaded groove. The cooperation between sleeve 10 and sliding sleeve 12 facilitates quick connection and disassembly of pipe 6, improving operational convenience. The locking steel ball 11 and sliding sleeve 12 abut against each other, ensuring the stability of the connection and improving the reliability of equipment operation. The inlet 5 is provided with an internal thread, and an external threaded cylinder is fixedly connected to the bottom end of the inner sleeve 10. A partition is provided between the external threaded cylinder and the inner sleeve 10. An outer sleeve extends from the partition at the outer wall of the inlet 5. Waterproof rubber is provided between the outer sleeve and the inlet 5. The screwing cooperation between the internal thread and the external threaded cylinder facilitates quick connection between the inlet 5 and pipe 6, improving operational convenience. The waterproof rubber design ensures the sealing of the connection, prevents culture medium leakage, and improves equipment safety.

[0020] Working principle: When using this utility model, first align the inner sleeve 10 at the end of the pipe 6 with the inlet 5. Initial fixation is achieved by screwing the external threaded sleeve into the internal thread of the inlet 5. Then, push the sliding sleeve 12 downwards, compressing the lifting spring. This causes the lower half of the sliding sleeve 12 to disengage from the locking ball 11, making the locking ball 11 face outwards, facilitating the smooth insertion of the pipe 6 into the inner sleeve 10. After the pipe 6 is inserted, release the sliding sleeve 12. The lifting spring pushes the sliding sleeve 12 upwards, causing the lower half of the sliding sleeve 12 to abut against the locking ball 11. The locking ball 11 moves inwards to abut against the outer wall of the pipe 6, locking it in place. The installation of the pipe 6 and the inlet 5 is then complete.

[0021] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. A perfusion device for stem cell culture, comprising a base (1), characterized in that: The base (1) has a mounting block on one side of its top surface, and a control panel (2) is hinged to one side of the top surface of the mounting block. A culture tank (3) is inserted into the middle of the top surface of the base (1). A sealing cap (4) is threaded onto the top surface of the culture tank (3). Multiple liquid inlets (5) are equidistantly arranged along the axial side of the top surface of the sealing cap (4). The liquid inlets (5) are connected to a pipe (6) through a connecting assembly. A culture medium bag (7) is provided at the end of the pipe (6). The connecting assembly includes an inner sleeve (10), on which a plurality of locking steel balls (11) are provided at equal intervals along the axial distance. A sliding sleeve (12) is provided outside the inner sleeve (10). A lifting spring is provided at the bottom end of the sliding sleeve (12). A limiting block is provided on the top surface of the inner sleeve (10). The lower half of the sliding sleeve (12) abuts against the locking steel balls (11). A springback groove is provided on the upper half of the sliding sleeve (12).

2. The perfusion device for stem cell culture according to claim 1, characterized in that: The culture medium bag (7) has an injection tube on one side of its top surface, and a bottle cap is screwed onto the injection tube. Multiple anti-slip patterns are evenly spaced on the bottle cap. The culture tank (3) has an outlet on one side of its bottom end.

3. The perfusion device for stem cell culture according to claim 1, characterized in that: Multiple mounting seats are provided at equal intervals on the opposite surface of the mounting block and the culture tank (3). A speed regulator (8) is installed on one of the mounting seats, and a pipe (6) is clamped and passed through the speed regulator (8).

4. The perfusion device for stem cell culture according to claim 1, characterized in that: The pipe (6) is made of plastic hose. A drive motor is provided in the middle of the top surface of the sealing cover (4). A rotating shaft is coaxially fixed on the output shaft of the drive motor. A stirring blade (9) is fixed at the bottom end of the rotating shaft.

5. The perfusion device for stem cell culture according to claim 1, characterized in that: The inlet (5) is provided with an internal thread, and the bottom end of the inner sleeve (10) is fixedly provided with an external threaded cylinder. A partition is provided between the external threaded cylinder and the inner sleeve (10). An outer sleeve extends from the partition at the outer wall of the inlet (5). Waterproof rubber is provided between the outer sleeve and the inlet (5).

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