Tumor marker detection device based on sers spectrum technology

Abstract

The utility model belongs to the technical field of marker detection, and particularly relates to a tumor marker detection device based on a sers spectrum technology, which comprises a treatment bin, a partition plate is arranged on the inner side of the treatment bin and divides the inside of the treatment bin into an adsorption bin and a cleaning bin, an adsorption structure is arranged in the adsorption bin, and a second drainage pipe is arranged at the bottom of the adsorption bin. A central column is fixed to the position, located at the center of the partition plate, in the treatment bin, a driving structure used for driving the adsorption structure to move into the cleaning bin from the interior of the adsorption bin is installed at the top of the central column, a detection bin is installed at the bottom of the cleaning bin, and a first drainage pipe is installed at the bottom of the detection bin; a detection assembly for detecting tumor markers is mounted in the detection bin, the adsorption structure comprises an adsorption plate, and first electromagnets are uniformly arranged in the adsorption plate. According to the utility model, the target object can be accurately separated, useless body fluid can be discharged, impurity interference can be obviously reduced through cleaning, and the detection accuracy can be greatly improved.

Description

Technical Field

[0001] This invention belongs to the field of biomarker detection technology, specifically relating to a tumor biomarker detection device based on SERS spectroscopy technology. Background Technology

[0002] Tumor marker detection plays a crucial role in the early diagnosis of tumors and is of great significance for improving the survival rate and treatment effect of cancer patients. At present, surface enhanced Raman spectroscopy (SERS) technology has attracted much attention in the field of tumor marker detection due to its outstanding advantages such as high detection sensitivity and the ability to provide component fingerprint information.

[0003] In existing tumor marker detection devices based on SERS spectroscopy, it is possible to detect tumor markers. For example, some devices construct specific SERS substrates and utilize the enhancement effect of metal structure surfaces to greatly amplify the Raman signal when molecules are adsorbed on metal colloidal nanoparticles or rough metal surfaces, thereby enabling the detection of low-concentration tumor markers.

[0004] However, in actual testing, clinical samples are often complex in composition. The samples not only contain tumor markers, but also a large number of other impurities, such as various proteins, cell debris, metabolites, etc. These impurities can interfere with the detection of tumor markers and reduce the accuracy of the detection.

[0005] In addition, how to effectively separate and enrich tumor markers during the detection process is also a major challenge. Traditional detection methods are difficult to accurately separate tumor markers from complex body fluids, which means that useless body fluid components will interfere with the detection signal during the detection, greatly reducing the reliability of the detection results.

[0006] Based on this, the present invention aims to improve existing tumor marker detection devices based on SERS spectroscopy technology. Utility Model Content

[0007] The purpose of this invention is to provide a tumor marker detection device based on SERS spectral technology, which can accurately separate target substances and remove useless body fluids. After cleaning, the interference of impurities is significantly reduced, and the detection accuracy is greatly improved.

[0008] The specific technical solution adopted by this utility model is as follows:

[0009] A tumor marker detection device based on SERS spectroscopy technology includes a processing chamber, and a partition is provided on the inner side of the processing chamber to divide the interior of the processing chamber into an adsorption chamber and a washing chamber.

[0010] The adsorption chamber is equipped with an adsorption structure, and a second drain pipe is provided at the bottom of the adsorption chamber. A central column is fixed in the processing chamber and at the center of the partition. A driving structure for moving the adsorption structure from the adsorption chamber to the cleaning chamber is installed on the top of the central column. A detection chamber is installed at the bottom of the cleaning chamber, and a first drain pipe is installed at the bottom of the detection chamber.

[0011] The detection chamber is equipped with a detection component for detecting tumor markers.

[0012] The adsorption structure includes an adsorption plate, and a first electromagnet is uniformly arranged inside the adsorption plate.

[0013] The upper surface of the adsorption plate is provided with a cross-section.

[0014] The adsorption plate has uniformly arranged leakage holes inside.

[0015] The drive structure includes a rotating plate rotatably connected to the top of the central column, a half gear fixed to the outside of the rotating plate, a rotating gear meshing with the outside of the half gear, a motor installed on the outside of the processing chamber, and the output end of the motor vertically upward connected to the rotating gear through a connecting rod.

[0016] An electric push rod is installed inside the rotating plate and at a position away from the center of the rotating plate. An adsorption iron plate is fixed vertically downward on the stroke rod of the electric push rod. The adsorption iron plate is attached to the center of the top of the adsorption plate and is attracted by the first electromagnet.

[0017] The detection assembly includes a second electromagnet installed inside the detection chamber, a protective cover installed inside the detection chamber and outside the second electromagnet, and a laser emitter installed inside the detection chamber and diagonally above the protective cover.

[0018] The top of the protective cover is sloped.

[0019] The technical effects achieved by this utility model are as follows:

[0020] This invention utilizes magnetic bead probes to specifically adsorb tumor markers, enabling the separation of tumor markers from complex bodily fluid environments. Subsequently, the magnetic beads are adsorbed by an adsorption plate and transferred to a cleaning solution for washing. Finally, the tumor markers are detected by laser, thereby accurately separating the target substances and removing useless bodily fluids. The washing process significantly reduces interference from impurities and greatly improves the accuracy of detection. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the structure of this utility model;

[0022] Figure 2 This is a cross-sectional view of the processing chamber in this utility model;

[0023] Figure 3 This is a schematic diagram of the structure between the processing chamber, the rotating plate, and the adsorption plate in this utility model;

[0024] Figure 4 This is a front view of the adsorption plate in this utility model;

[0025] Figure 5 This is a cross-sectional view of the adsorption plate in this utility model;

[0026] Figure 6 This is a cross-sectional view of the detection chamber in this utility model.

[0027] The attached diagram lists the components represented by each number as follows:

[0028] 1. Processing chamber; 2. Partition; 3. Adsorption chamber; 4. Cleaning chamber; 5. Adsorption plate; 6. Leakage hole; 7. First electromagnet; 8. Cross-section; 9. Central column; 10. Rotating plate; 11. Half gear; 12. Rotating gear; 13. Motor; 14. Electric push rod; 15. Adsorption iron plate; 16. Detection chamber; 17. Second electromagnet; 18. First drain pipe; 19. Protective cover; 20. Laser emitter; 21. Second drain pipe. Detailed Implementation

[0029] To make the objectives and advantages of this utility model clearer, the following detailed description is provided in conjunction with embodiments. It should be understood that the following text is merely used to describe one or more specific embodiments of this utility model and does not strictly limit the scope of protection specifically claimed by this utility model.

[0030] like Figures 1-6 As shown, a tumor marker detection device based on SERS spectroscopy technology includes a processing chamber 1. A partition 2 is provided on the inner side of the processing chamber 1, which divides the interior of the processing chamber 1 into an adsorption chamber 3 and a washing chamber 4. The adsorption chamber 3 contains multiple magnetic beads with probes on them, which are used to identify and adsorb tumor markers. The surface of the magnetic beads is modified with antibodies or aptamers that specifically identify tumor markers by chemical coupling, which are used to accurately identify and adsorb tumor markers. The obtained samples, such as blood or urine, are introduced into the adsorption chamber 3, and the corresponding tumor markers are adhered to by the magnetic beads.

[0031] The adsorption chamber 3 is equipped with an adsorption structure, and a second drain pipe 21 is installed at the bottom of the adsorption chamber 3, as shown in the attached diagram. Figures 4-5The adsorption structure includes an adsorption plate 5, within which first electromagnets 7 are uniformly arranged. After the magnetic beads adsorb the corresponding tumor markers, the first electromagnets 7 in the leakage hole 6 are activated, generating magnetic force to adsorb the magnetic beads that have adsorbed the tumor markers, causing the magnetic beads to adhere to the outer wall of the adsorption plate 5. A liquid pump or solenoid valve is installed on the second drain pipe 21. By activating the liquid pump or solenoid valve, the remaining body fluid is discharged through the second drain pipe 21, while the magnetic beads remain on the adsorption plate 5. (Refer to attached diagram). Figure 4 The upper surface of the adsorption plate 5 is provided with a cut surface 8. The cut surface 8 allows body fluid to flow downward through the cut surface 8, reducing the amount of body fluid remaining on the upper surface of the adsorption plate 5. According to the above structure, the interior of the adsorption plate 5 is uniformly provided with leakage holes 6. The leakage holes 6 allow body fluid to be discharged not only through the edge of the adsorption plate 5, but also through the leakage holes 6, further enhancing the body fluid discharge effect.

[0032] A central column 9 is fixed inside the processing chamber 1 and at the center of the partition 2. A driving structure for moving the adsorption structure from the adsorption chamber 3 to the cleaning chamber 4 is installed on the top of the central column 9. A detection chamber 16 is installed at the bottom of the cleaning chamber 4. A first drain pipe 18 is installed at the bottom of the detection chamber 16.

[0033] Furthermore, the drive structure includes a rotating plate 10 rotatably connected to the top of the central column 9, a half gear 11 fixed to the outside of the rotating plate 10, a rotating gear 12 meshing with the outside of the half gear 11, and a motor 13 installed on the outside of the processing chamber 1. The output end of the motor 13 is vertically upward and connected to the rotating gear 12 through a connecting rod.

[0034] An electric push rod 14 is installed inside the rotating plate 10 and at a position away from the center of the rotating plate 10. An adsorption iron plate 15 is fixed vertically downward on the stroke rod of the electric push rod 14. The adsorption iron plate 15 is attached to the center of the top of the adsorption plate 5 and is adsorbed by the first electromagnet 7.

[0035] After the body fluid is discharged from the adsorption chamber 3, the electric push rod 14 can be driven to move the adsorption iron sheet 15 until the adsorption iron sheet 15 moves to be in contact with the adsorption plate 5. Through the continuous activation of the first electromagnet 7, the adsorption iron sheet 15 is adsorbed on the adsorption plate 5. The stroke rod of the electric push rod 14 is retracted, and the adsorption plate 5 is moved to the top of the treatment chamber 1.

[0036] The motor 13 is started, causing the output end of the motor 13 to drive the rotating gear 12 to rotate. Through the meshing connection between the rotating gear 12 and the half gear 11, the rotating gear 12 can drive the half gear 11 and the rotating plate 10 to rotate, thereby driving the electric push rod 14 and the adsorption plate 5 to rotate to be directly above the cleaning chamber 4. The cleaning chamber 4 is filled with cleaning fluid such as physiological saline or phosphate buffer. The electric push rod 14 sends the adsorption iron plate 15 and the adsorption plate 5 into the cleaning chamber 4. The magnetic beads are cleaned by the cleaning fluid inside the cleaning chamber 4, making it easier to detect and observe. By closing the first electromagnet 7, the adsorption plate 5 loses control over the magnetic beads and the adsorption plate 5. At this time, the adsorption plate 5 and the electric push rod 14 are reset. The magnetic beads are cleaned of impurities in the cleaning fluid. The first drain pipe 18 is equipped with a liquid pump or solenoid valve. By starting the liquid pump or solenoid valve, the cleaning fluid is discharged through the second drain pipe 21. The detection chamber 16 is equipped with a detection component for detecting tumor markers.

[0037] See attached document Figure 6 The detection assembly includes a second electromagnet 17 installed inside the detection chamber 16. A protective cover 19 is installed inside the detection chamber 16 and outside the second electromagnet 17. A laser emitter 20 is installed inside the detection chamber 16 and diagonally above the protective cover 19. The laser emitter 20 can be any laser device that can emit lasers capable of detecting tumor markers, such as a solid-state laser or a semiconductor laser. A protective housing can be installed on the outside of the laser emitter 20. The protective housing can be made of transparent material and will not affect the laser emission.

[0038] When the cleaning fluid is discharged, it flows out through the detection chamber 16. At this time, the second electromagnet 17 is activated, causing it to attract the magnetic beads, which are then adsorbed onto the protective cover 19. The top of the protective cover 19 is sloped, allowing the cleaning fluid to flow down the slope. The magnetic beads remain on the protective cover 19 due to the attraction of the second electromagnet 17. This drives the laser emitter 20, causing it to emit laser light. After the tumor markers and probes are combined, characteristic spectra are collected by a Raman spectrometer. Qualitative and quantitative analysis is achieved by combining chemometric algorithms. This device is similar to other existing detection devices and can also be connected to an external control host and analysis controller to analyze the detection results. This setup removes most impurities and body fluids that are not necessary for detection, focusing only on the tumor markers adsorbed by the magnetic bead probes, thus ensuring the accuracy of the detection.

[0039] The above description is merely a preferred embodiment of this utility model. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of this utility model, and these improvements and modifications should also be considered within the scope of protection of this utility model. Structures, devices, and operating methods not specifically described or explained in this utility model, unless otherwise specified or limited, shall be implemented using conventional methods in the field.

Claims

1. A tumor marker detection device based on SERS spectroscopy technology, comprising a processing chamber (1), characterized in that: The processing chamber (1) is provided with a partition (2) on its inner side, which divides the interior of the processing chamber (1) into an adsorption chamber (3) and a cleaning chamber (4). An adsorption structure is provided inside the adsorption chamber (3), and a second drain pipe (21) is provided at the bottom of the adsorption chamber (3). A central column (9) is fixed inside the treatment chamber (1) and at the center of the partition (2). A driving structure is installed on the top of the central column (9). The driving structure is used to drive the adsorption structure to move from the adsorption chamber (3) to the cleaning chamber (4). A detection chamber (16) is installed at the bottom of the cleaning chamber (4), and a first drain pipe (18) is installed at the bottom of the detection chamber (16). The detection chamber (16) is equipped with a detection component for detecting tumor markers.

2. The tumor marker detection device based on SERS spectroscopy technology according to claim 1, characterized in that: The adsorption structure includes an adsorption plate (5), and a first electromagnet (7) is uniformly arranged inside the adsorption plate (5).

3. The tumor marker detection device based on SERS spectroscopy technology according to claim 2, characterized in that: The upper surface of the adsorption plate (5) is provided with a cut surface (8).

4. The tumor marker detection device based on SERS spectroscopy technology according to claim 2, characterized in that: The adsorption plate (5) has uniformly arranged leakage holes (6) inside.

5. The tumor marker detection device based on SERS spectroscopy technology according to claim 2, characterized in that: The drive structure includes a rotating plate (10) rotatably connected to the top of the central column (9), a half gear (11) is fixed on the outside of the rotating plate (10), a rotating gear (12) is meshed on the outside of the half gear (11), and a motor (13) is installed on the outside of the processing chamber (1). The output end of the motor (13) is vertically upward and connected to the rotating gear (12) through a connecting rod. An electric push rod (14) is installed inside the rotating plate (10) and at a position away from the center of the rotating plate (10). An adsorption iron plate (15) is fixed vertically downward on the stroke rod of the electric push rod (14). The adsorption iron plate (15) is attached to the center of the top of the adsorption plate (5) and is adsorbed by the first electromagnet (7).

6. The tumor marker detection device based on SERS spectroscopy technology according to claim 1, characterized in that: The detection assembly includes a second electromagnet (17) installed inside the detection chamber (16), a protective cover (19) installed inside the detection chamber (16) and outside the second electromagnet (17), and a laser emitter (20) installed inside the detection chamber (16) and diagonally above the protective cover (19).

7. The tumor marker detection device based on SERS spectroscopy technology according to claim 6, characterized in that: The top of the protective cover (19) is sloped.

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