Double-cover type square culture dish

Abstract

The double-cover type square culture dish comprises a dish body and two cover bodies, namely a cover body A and a cover body B, used in cooperation with the dish body, the dish body is of a box body structure with an opening in the top, and the cover body A and the cover body B are of a box body structure with an opening in the bottom and used for covering the opening of the dish body. A plurality of division bars protruding into the dish body are further arranged on the inner surface of the cover body B, and gaps are formed between the division bars and the inner bottom surface of the dish body. The culture dish not only solves the problem that liquid drop paths are crossed in the process of inclining the dish body when a common culture dish is used for carrying out a bactericidal antibody satisfaction detection experiment, but also improves the accuracy of an experiment result, and has the characteristics of simple structure, low cost, convenience and practicability.

Description

Technical Field

[0001] This utility model provides a double-lid square petri dish, specifically relating to experimental equipment for culturing microorganisms on solid culture media, and belongs to the field of experimental equipment technology. Background Technology

[0002] In the development of bacterial vaccines, it is usually necessary to evaluate the immunogenicity of serum after immunization of animals or humans. A key experiment is to detect the level of vaccine-induced bactericidal antibody titers against the target bacteria in the serum. In the bactericidal antibody titer detection experiment, bacteria, complement, and serially diluted serum are mixed, and the incubated mixture is added dropwise to a culture plate containing a specific antibiotic. The plate is tilted to allow the liquid to flow in a strip. After incubation under suitable conditions for a period of time, surviving bacteria form visible colonies. The number of surviving colonies is counted using an automatic counter or manually. Using the colony count in the complement control well without serum as a baseline, the bactericidal titer is defined as the highest serum dilution that kills 50% of the bacteria. The experimental results, from left to right, show that as the serum dilution gradient increases, the bactericidal effect of the serum decreases, leading to the gradual appearance of bacterial colonies, thus allowing for the determination of the serum bactericidal titer.

[0003] In the actual operation of the bactericidal antibody titer detection experiment, using a regular round petri dish, only the wider central portion can be utilized for the experiment. Typically, only a limited number of mixed droplets can be added for comparative experiments, resulting in low throughput. Furthermore, with a regular square petri dish, during the tilting process after adding the mixed droplets, it is difficult to ensure that the droplets flow steadily and evenly to form a uniformly distributed colony band. The lack of obstruction between droplets at different positions makes them prone to crossing over after tilting, leading to unclear colony bands after incubation and severely affecting the interpretation of experimental results.

[0004] Most existing technologies divide the petri dish into multiple culture wells by setting partitions within the dish's space. For example, the utility model patent with publication number CN209619350U discloses a partitioned biological culture dish, which only divides the main body of the dish into two parts, failing to meet the aforementioned experimental requirements. Another example is the utility model patent with publication number CN220550172U, which discloses a disposable, detachable, partitioned square culture dish with a grid-like multi-well design; neither of these methods can form multiple clear colony bands. Therefore, it is evident that existing technologies do not provide technical solutions to the aforementioned or related problems. Utility Model Content

[0005] The purpose of this invention is to utilize a cover B, which works in conjunction with the petri dish and has several internal partitions, to allow the solid culture medium that melts inside the petri dish to solidify and form spaced grooves that can be used to cultivate bacterial colonies. This solves the problem that ordinary petri dishes cannot form clear, non-crossing bacterial colonies in the bactericidal antibody titer detection experiment, thereby ensuring the reliability of the experimental results.

[0006] This utility model is achieved through the following technical solution:

[0007] A double-lid square petri dish includes a dish body and two lids for use with the dish body: lid A and lid B. The dish body is a box structure with an open top, and lids A and B are box structures with open bottoms for covering the opening of the dish body. The inner surface of lid B is also provided with several protruding partitions into the dish body, and there is a gap between the partitions and the bottom surface of the dish body.

[0008] Preferably, the spacer is a strip-shaped structure, preferably a cuboid structure; the interior of the strip-shaped structure can be solid or hollow; the four corners of the strip-shaped structure protruding into the dish can be right-angled or rounded.

[0009] Preferably, the width of the spacer is 5-25mm (more preferably 10-20mm), and the length of the spacer is 10-30mm shorter than the side length of the dish body.

[0010] Preferably, the height of the spacer is 3 / 5 to 4 / 5 (more preferably 2 / 3 to 4 / 5) of the depth of the dish (referring to the vertical distance between the highest point of the side wall of the dish and the inner bottom surface); correspondingly, the height of the gap between the spacer and the inner bottom surface of the dish is 1 / 5 to 2 / 5 (more preferably 1 / 5 to 1 / 3) of the depth of the dish.

[0011] Preferably, the spacers are spaced apart and parallel to the side wall of the cover B in which they are located.

[0012] Preferably, the spacers are evenly distributed within the cover B, and the distance between two adjacent spacers is 5-10 mm.

[0013] Preferably, the number of spacers is 3 to 10.

[0014] Preferably, the dish body and both lids are made of plastic or glass.

[0015] Preferably, the spacer strip is integrally formed with the cover body B.

[0016] Compared with the prior art, this utility model has the following advantages and beneficial effects:

[0017] (1) This utility model provides several protruding partitions on one of the cover bodies B that extend into the dish body, and sets a gap between the partitions and the bottom surface of the dish body, so that the heated and melted culture medium poured into the dish body forms several strip-shaped grooves after solidification. The mixed droplets are dripped into the grooves respectively, and then the operation is carried out according to the experimental requirements to obtain clear and non-crossing colony bands, so as to ensure the reliability of the experimental results.

[0018] (2) By setting a strip-shaped partition, the present invention can form a groove in the strip-shaped structure after the culture medium solidifies, which is conducive to the flow of the measured droplets in the groove to form a bacterial liquid band.

[0019] (3) By setting the width of the spacer to 5~25mm and the length to be 10~30mm shorter than the side length of the dish, the present invention can form a bacterial culture groove with a suitable area and a suitable number after the culture medium solidifies, so that it is suitable for bacterial growth and can obtain as many grooves as possible to improve the detection throughput.

[0020] (4) By setting the height of the septum to 3 / 5 to 4 / 5 (preferably 2 / 3 to 4 / 5) of the depth of the dish, the appropriate septum height ensures that the gap between it and the bottom of the dish is of appropriate size (the latter determines the thickness of the culture medium in the groove formed after the culture medium solidifies), thus ensuring the effectiveness of the experiment while also being economical: 1) On the one hand, it avoids the culture medium thickness in the groove being too small due to the excessive height of the septum, which would be unsuitable for bacterial survival or easily dry out during the culture process; 2) On the other hand, it avoids the culture medium in the groove being too thick due to the excessive height of the septum, thus avoiding the waste of raw materials: When preparing the plate, the amount of culture medium poured in needs to make the liquid surface on top higher than the bottom of the septum (about 1 to 5 mm above the bottom), so the appropriate septum height ensures that the required groove can be obtained to accommodate the test droplets without pouring in too much culture medium, thus ensuring the simplicity and economy of the experimental operation.

[0021] (5) By setting the height of the gap to 1 / 5 to 2 / 5 (preferably 1 / 5 to 1 / 3) of the depth of the dish, the protruding partition strip maintains a suitable gap with the bottom surface of the dish after the cover B is placed on the dish into which the hot-melted culture medium has been poured. As a result, the culture medium at the bottom of the groove is of suitable thickness, which can meet the requirements of bacterial culture and is not easily broken.

[0022] (6) By setting the spacers at intervals so that they are parallel to the side wall of the dish and are evenly distributed within the cover B, this utility model ensures that the formed grooves are evenly distributed within the dish, so as to form clear, non-crossing colony bands, thereby ensuring the reliability of the experimental results. Setting the interval between two adjacent spacers to 5~10mm can avoid the waste of space and materials caused by too large an interval, and at the same time avoid the situation where the culture medium thickness between adjacent grooves is too thin due to too small an interval, which would easily damage the formed groove structure when the cover B is removed. Thus, more grooves with stable shapes can be obtained as much as possible within a limited space.

[0023] (7) By making the dish body and the two types of lids both made of plastic or glass, this utility model not only facilitates irradiation sterilization and reduces the cost of disposable experimental equipment (such as using plastic material), but also facilitates high temperature and high pressure sterilization and reuse of the culture dish (such as using glass material).

[0024] (8) By integrally molding the partition strip and the cover B, this utility model ensures the stability of the structure and avoids the gap structure between the top of the partition strip and the plane of the cover B, so as to prevent incomplete disinfection due to the gap structure during the disinfection process.

[0025] In summary, this invention features a simple structure, low cost, and ease of use. It not only solves the problem of droplet path intersection during the tilting of ordinary petri dishes in the prior art for detecting bactericidal antibody titers, but also ensures that the colony bands formed by this invention have clear and distinct boundaries, thereby improving the accuracy of experimental results and possessing high practical value. Attached Figure Description

[0026] Figure 1 This is a schematic diagram of the structure of this utility model;

[0027] Figure 2 This is a cross-sectional view of the dish body and the cap body B used together in this utility model (after the poured culture medium has solidified).

[0028] Figure 3 This is a schematic diagram of the structure of the dish in this utility model (after the poured culture medium has solidified).

[0029] Figure 4 This is a top view of the dish in this invention (after the poured culture medium has solidified).

[0030] Wherein, 1—vessel body, 2—lid body A, 3—lid body B, 4—spacer, 5—gap, 6—groove. Detailed Implementation

[0031] The present invention will be further described in detail below with reference to the embodiments, but the implementation of the present invention is not limited thereto.

[0032] Example:

[0033] like Figures 1 to 4 As shown in the structure, this embodiment discloses a double-lid square culture dish, including a dish body 1 and two lids used in conjunction with the dish body 1: lid A2 and lid B3. The dish body 1 is a box structure with an open top, and the two lids (i.e., lid A2 and lid B3) are box structures with open bottoms to cover the opening of the dish body 1. The inner surface of lid B3 is also provided with several spacers 4 protruding into the dish body 1, and the spacers 4 and the inner bottom surface of the dish body 1 are provided with gaps 5.

[0034] The use of this embodiment includes two parts:

[0035] (1) Experimental preparation stage: using, for example Figure 1 The following image shows dish 1 and Figure 1 The cover B3, which has a spacer 4, shown in the middle figure, is completed by following these steps:

[0036] Step 101: First, pour the heated and melted liquid culture medium into dish 1;

[0037] Step 102: Cover the dish body 1 with the cover body B3, which has the spacer strip 4, so that the lower end of the spacer strip 4 is submerged below the surface of the culture medium liquid (submerged to about 1~5mm, preferably 1~2mm).

[0038] Step 103: After allowing the culture medium to cool and solidify, remove the cover B3. A solid culture medium in the dish 1 will then form a surface resembling... Figure 3 and Figure 4 The groove 6 shown.

[0039] (2) Experimental stage: using the mixture formed in step 103 as shown in the figure. Figure 3 and Figure 4 The dish shown is 1 and Figure 1 The other cover A2 shown in the diagram above, which does not have a spacer 4, is completed by following these steps:

[0040] Step 201: Drop the mixture prepared according to the experimental requirements into one end of each groove 6 in the horizontally placed dish 1;

[0041] Step 202: Lift the dish 1 close to one end of the groove 6 into which the mixture has been dropped, and tilt it to the required angle for the experiment, so that the droplets in the groove 6 flow along the groove 6 to the other end.

[0042] Step 203: Place dish 1 back in a horizontal position, cover it with the lid A2, and let it stand according to the experimental requirements;

[0043] Step 204: Record, compare, and analyze the data at the required time points to obtain the desired experimental results.

[0044] In the usage method, the experimental steps in the second part are routine operations and will not be described again here; the groove 6 formed on the solid culture medium in the experimental preparation stage of the first part is an effective guarantee for the formation of non-crossing and clear colony bands in the experimental stage.

[0045] Furthermore, to ensure the reliability of the experimental results, the following methods and measures were adopted:

[0046] (1) The spacer 4 is set as a strip structure, specifically a cuboid structure.

[0047] (2) The width of the spacer 4 is set to 5~25mm (the optimal width is 10~20mm), and the length is set to be 10~30mm shorter than the side length of the dish body 1; taking a square culture dish with a side length of 130mm as an example, the length of the spacer 4 is set to 100~120mm.

[0048] (3) The height of the gap 5 is set to 3 / 5 to 4 / 5 (preferably 2 / 3 to 4 / 5) of the height of the dish body 1. Correspondingly, the height of the gap 5 is 1 / 5 to 2 / 5 (preferably 1 / 5 to 1 / 3) of the depth of the dish body 1. Taking a culture dish with a depth of 15 mm as an example, the height of the spacer 4 is set to 9 to 12 mm (10 to 12 mm is better) so that after the cover B3 is placed on the dish body 1 which has been filled with sufficient hot melt culture medium, the bottom of the spacer and the bottom surface of the dish body 1 maintain a gap of 3 to 6 mm (3 to 5 mm is better) and the liquid surface of the culture medium overflows the bottom of the spacer by 1 to 5 mm.

[0049] (4) The spacers 4 are spaced apart and parallel to the side wall of the dish body 1; the number of spacers 4 is set to 3 to 10.

[0050] (5) Distribute the spacers 4 evenly within the cover body B3 and set the interval between two adjacent spacers 4 to 5~10mm.

[0051] This effectively ensures that the groove 6 can maintain an effective culture space, which is used to form a uniformly distributed, non-crossing and clear colony band, thereby ensuring the reliability of the experimental results.

[0052] Furthermore, to facilitate disinfection and save costs, or to facilitate reuse, the dish body 1 and the two types of lids can be made of plastic or glass. These materials are inexpensive to manufacture, have good transparency for monitoring and recording the experimental process, and can effectively ensure the smooth progress of the experiment. Of course, any material that ensures the normal use of this invention can be used in this embodiment.

[0053] Furthermore, to ensure structural stability and avoid gaps, the spacer 4 can be integrally formed with the cover B3.

[0054] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Any simple modifications or equivalent changes made to the above embodiments based on the technical essence of the present utility model shall fall within the protection scope of the present utility model.

Claims

1. A double-lid square petri dish, characterized in that, Includes a dish body (1) and two lids used in conjunction with the dish body (1): lid A (2) and lid B (3); the dish body (1) is a box structure with an open top, and the lid A (2) and lid B (3) are box structures with an open bottom for covering the opening of the dish body (1). The inner surface of the lid B (3) is also provided with several partitions (4) protruding into the dish body (1), and the partitions (4) and the inner bottom surface of the dish body (1) are provided with a gap (5).

2. The double-lid square petri dish according to claim 1, characterized in that, The spacer (4) has a strip-shaped structure.

3. The double-lid square petri dish according to claim 2, characterized in that, The strip structure is a cuboid structure.

4. The double-lid square petri dish according to claim 3, characterized in that, The width of the spacer (4) is 5~25mm, and the length of the spacer (4) is 10~30mm shorter than the side length of the dish body (1).

5. The double-lid square petri dish according to claim 4, characterized in that, The width of the spacer (4) is 10~20mm.

6. The double-lid square petri dish according to claim 3, characterized in that, The height of the spacer (4) is 3 / 5 to 4 / 5 of the depth of the dish (1).

7. The double-lid square petri dish according to claim 6, characterized in that, The height of the spacer (4) is 2 / 3 to 4 / 5 of the depth of the dish (1).

8. The double-lid square petri dish according to claim 1, characterized in that, The spacers (4) are spaced apart and are parallel to the side wall of the cover B (3).

9. The double-lid square petri dish according to claim 8, characterized in that, The spacers (4) are evenly distributed within the cover body B (3), and the distance between two adjacent spacers (4) is 5~10mm.

10. The double-lid square petri dish according to claim 8, characterized in that, The number of the spacers (4) is 3 to 10.

11. The double-lid square petri dish according to claim 1, characterized in that, The dish body (1) and both lids are made of plastic or glass.

12. The double-lid square petri dish according to any one of claims 1 to 11, characterized in that, The spacer (4) is integrally formed with the cover B (3).

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