Efficient continuous culture device for AKK bacteria

Abstract

The utility model discloses a high-efficiency continuous culture device for AKK bacteria, which belongs to the technical field of anaerobic bacteria culture and comprises an anaerobic incubator and an anaerobic fermentation tank located inside the anaerobic incubator. A mother liquor tank and three culture medium preparation tanks are arranged beside the anaerobic fermentation tank, culture medium supply mechanisms are arranged on the mother liquor tank, the three culture medium preparation tanks and the anaerobic fermentation tank, and the mother liquor tank and the three culture medium preparation tanks are communicated with the anaerobic fermentation tank through the culture medium supply mechanisms; the top of the anaerobic fermentation tank is provided with a gas conveying mechanism, the gas conveying mechanism is communicated with the anaerobic fermentation tank, and the bottom of the anaerobic fermentation tank is provided with a discharging channel. According to the AKK bacteria culture device, culture conditions can be adjusted in time, each batch of AKK bacteria can grow under the same optimal condition, the uniformity and the stability of product quality are effectively ensured, and the product quality is improved.

Description

Technical Field

[0001] This invention belongs to the field of anaerobic bacteria culture technology, and particularly relates to a high-efficiency continuous culture device for AKK bacteria. Background Technology

[0002] Akkermansia muciniphila, an important probiotic in the gut, has shown great potential in regulating gut microbiota balance, enhancing host immune function, and improving metabolic diseases. Its related products have broad application prospects in biomedicine, functional foods, and other fields. However, the industrial cultivation of Akkermansia muciniphila currently faces many technical bottlenecks.

[0003] Traditional AKK bacteria cultivation often employs batch culture methods, which have significant drawbacks. Firstly, during batch culture, the culture environment undergoes drastic changes due to nutrient consumption and metabolic product accumulation, making it difficult to maintain optimal growth conditions for AKK bacteria over extended periods. This results in low cell density, low production efficiency, and an inability to meet growing market demand. Secondly, batch culture makes precise control of the cultivation process difficult, hindering the guarantee of product quality uniformity and stability.

[0004] In the preparation and delivery of culture media, traditional equipment often lacks the ability to precisely control the proportions. AKK bacteria are extremely sensitive to the ratio of nutrients; improper proportions of carbon sources, nitrogen sources, and specific growth factors can severely affect their growth, metabolism, and activity. Traditional equipment cannot dynamically and precisely supply nutrients according to the growth characteristics of AKK bacteria, resulting in low nutrient utilization efficiency and high culture costs.

[0005] Furthermore, AKK bacteria are strict anaerobic bacteria, requiring specific oxygen levels in their culture environment. Ordinary anaerobic culture equipment is inadequate in terms of gas replacement and environmental maintenance, making it difficult to guarantee a stable anaerobic environment over extended periods. Even a tiny amount of oxygen infiltration can inhibit AKK growth or even lead to bacterial death. Simultaneously, parameters such as temperature and pH levels within the equipment are difficult to maintain stably, further impacting the cultivation results of AKK bacteria. Utility Model Content

[0006] This invention provides a high-efficiency continuous culture device for AKK bacteria to solve the problems in the prior art.

[0007] The present invention adopts the following technical solution: a high-efficiency continuous culture device for AKK bacteria, including an anaerobic incubator and an anaerobic fermenter, wherein the anaerobic fermenter is located inside the anaerobic incubator; a mother liquor tank and three culture medium preparation tanks are provided on the side of the anaerobic fermenter, and the mother liquor tank and the three culture medium preparation tanks are all provided with culture medium supply mechanisms. The mother liquor tank and the three culture medium preparation tanks are connected to the anaerobic fermenter through the culture medium supply mechanisms. A gas conveying mechanism is provided at the top of the anaerobic fermenter and is connected to the anaerobic fermenter. A discharge channel is provided at the bottom of the anaerobic fermenter.

[0008] In a further technical solution, each of the culture medium supply devices includes a peristaltic pump, an inlet pipe, and an outlet pipe. The peristaltic pump is connected to the side wall of the anaerobic fermenter. The two ends of the outlet pipe are respectively connected to the peristaltic pump and the anaerobic fermenter. The inlet pipe is respectively connected to the peristaltic pump and the anaerobic fermenter or the mother liquor tank.

[0009] A further technical solution is to install a flow meter on the liquid outlet pipe.

[0010] In a further technical solution, the bottom of the anaerobic fermenter is provided with a motor base, the motor base is provided with a stirring motor, the main shaft of the stirring motor is provided with a vertically arranged stirring shaft, the stirring shaft extends into the interior of the anaerobic fermenter, and the stirring shaft is provided with several stirring rods.

[0011] A further technical solution is that the anaerobic fermenter is equipped with a pressure gauge, a pH probe, and a biosensor analyzer.

[0012] In a further technical solution, the gas delivery mechanism is equipped with a mass flow meter.

[0013] In a further technical solution, a gas replacement machine and a circulating fan are provided on the side wall of the anaerobic incubator, and an oxygen sensor and a carbon dioxide sensor are provided on the inner side wall of the anaerobic incubator. The gas replacement machine and the circulating fan are electrically connected to the oxygen sensor and the carbon dioxide sensor.

[0014] A further technical solution is that the discharge channel is equipped with a closed opening and closing door.

[0015] A further technical solution is that the anaerobic incubator has a sealed door on its side wall.

[0016] A further technical solution involves three culture medium preparation tanks, each containing a liquid storage tank for traditional Chinese medicine culture medium.

[0017] This invention provides a high-efficiency continuous culture device for AKK bacteria, which has the following beneficial effects:

[0018] I. Precise Nutrition Regulation, Breaking Through Traditional Culture Bottlenecks: Addressing the problem that traditional equipment cannot accurately proportion nutrients, this invention uses an independent mother liquor tank and three culture medium preparation tanks, along with a precise culture medium supply mechanism, to dynamically and precisely deliver various nutrients according to the growth characteristics of AKK bacteria. This completely solves the problem of poor bacterial growth caused by nutrient imbalance, improves nutrient utilization efficiency, and reduces culture costs.

[0019] II. Stabilizing the Anaerobic Environment and Ensuring Cell Viability: Given the high sensitivity of AKK bacteria to oxygen and the shortcomings of traditional anaerobic equipment in maintaining a stable environment, this equipment utilizes a gas exchanger and circulating fan in its anaerobic incubator to construct and maintain a stable anaerobic environment. The gas delivery mechanism and pressure gauges in the anaerobic fermenter work in tandem to regulate the internal gas environment and pressure in real time. This comprehensive environmental control effectively avoids the inhibitory or lethal effects of oxygen infiltration on AKK bacteria, providing a stable anaerobic environment for their growth and ensuring cell viability and a high success rate of cultivation.

[0020] 3. Continuous cultivation mode improves production efficiency: The equipment adopts a continuous cultivation mode to change the low efficiency of traditional batch cultivation. It achieves seamless connection between cultivation, material discharge and replenishment of culture medium, which greatly shortens the production cycle, reduces equipment downtime and significantly increases the yield of AKK bacteria per unit time.

[0021] IV. Dynamic Parameter Monitoring to Ensure Uniform Product Quality: Utilizing advanced monitoring devices such as biosensor analyzers and pH probes, key parameters during the fermentation process are collected in real time and dynamically adjusted according to preset standards. Compared to the difficulty of precisely controlling the cultivation process with traditional equipment, this invention can adjust cultivation conditions in a timely manner, ensuring that each batch of AKK bacteria grows under the same optimal conditions, effectively guaranteeing the uniformity and stability of product quality and improving overall product quality.

[0022] V. Enhanced Mixing and Mass Transfer, Optimized Cultivation Process: The stirring system ensures thorough mixing of the fermentation broth, preventing nutrient sedimentation and uneven local concentrations, and enhancing the mass transfer process. This is an improvement over the poor mixing effect of traditional equipment. By increasing the utilization rate of nutrients and avoiding inconsistent cell growth caused by local environmental differences, the entire cultivation process is optimized, further enhancing the cultivation effect and production efficiency of AKK bacteria. Attached Figure Description

[0023] The accompanying drawings, which are included to provide a further understanding of the present invention and constitute a part of this invention, illustrate exemplary embodiments of the present invention and, together with the description thereof, serve to explain the present invention and do not constitute an undue limitation thereof. In the drawings:

[0024] Figure 1 This is a schematic diagram of the three-dimensional structure of the present invention. Figure 1 ;

[0025] Figure 2 This is a three-dimensional structural diagram of the present invention;

[0026] Figure 3 This is a partial three-dimensional structural schematic diagram of the present invention;

[0027] Figure 4 This is a partial top view of the present invention;

[0028] Figure 5 This is a partial side view of the present invention;

[0029] Figure 6 This is a three-dimensional structural diagram of the anaerobic fermenter in this utility model;

[0030] Figure 7 This is a three-dimensional structural diagram of the culture medium supply mechanism in this utility model;

[0031] Figure Labels

[0032] 1. Anaerobic incubator, 11. Gas replacement machine, 12. Circulating fan, 13. Sealed door, 2. Anaerobic fermenter, 3. Mother liquor tank, 4. Culture medium preparation tank, 5. Culture medium supply mechanism, 51. Peristaltic pump, 52. Inlet pipe, 53. Outlet pipe, 6. Gas conveying mechanism, 7. Discharge channel, 71. Opening and closing door, 8. Motor base, 81. Stirring motor, 82. Stirring shaft, 83. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below in conjunction with specific embodiments and corresponding drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0034] The technical solutions provided by the various embodiments of this utility model are described in detail below with reference to the accompanying drawings.

[0035] Reference Figures 1 to 7As shown, this utility model embodiment provides a high-efficiency continuous culture device for AKK bacteria, including an anaerobic incubator 1 and an anaerobic fermenter 2. The anaerobic fermenter 2 is located inside the anaerobic incubator 1. A mother liquor tank 3 and three culture medium preparation tanks 4 are provided on the side of the anaerobic fermenter 2. The mother liquor tank 3 and the three culture medium preparation tanks 4 are all provided with a culture medium supply mechanism 5 on the anaerobic fermenter 2. The mother liquor tank 3 and the three culture medium preparation tanks 4 are connected to the anaerobic fermenter 2 through the culture medium supply mechanism 5. A gas conveying mechanism 6 is provided at the top of the anaerobic fermenter 2 and is connected to the anaerobic fermenter 2. A discharge channel 7 is provided at the bottom of the anaerobic fermenter 2.

[0036] Working principle:

[0037] Before culturing AKK bacteria, staff prepared the mother liquor and three types of traditional Chinese medicine culture media in the mother liquor tank 3 and the three types of traditional Chinese medicine culture media preparation tank 4, respectively. The mother liquor contains the basic nutrients necessary for the growth of AKK bacteria, while the traditional Chinese medicine culture media are supplemented with specific nutrients according to the growth requirements of AKK bacteria.

[0038] At the start of cultivation, the mother liquor tank 3 and the three traditional Chinese medicine culture medium preparation tanks 4, through their respective culture medium supply mechanisms 5, deliver the mother liquor and the three traditional Chinese medicine culture media to the anaerobic fermenter 2 according to a pre-set ratio and sequence. The culture medium supply mechanism 5 can precisely control the delivery volume and flow rate of each liquid to ensure that the composition of the mixed culture medium meets the optimal requirements for the growth of AKK bacteria.

[0039] Anaerobic fermenter 2 is located inside anaerobic incubator 1. Anaerobic incubator 1 uses gas exchanger 11 to replace the internal air with oxygen-free gas, quickly creating and maintaining a stable anaerobic environment. Circulating fan 12 ensures uniform gas distribution within the chamber, guaranteeing consistent temperature, humidity, and gas composition, providing a stable external growth environment for AKK bacteria.

[0040] The gas delivery mechanism 6 at the top of the anaerobic fermenter 2 introduces gases such as carbon dioxide, which are necessary for the growth of AKK bacteria, into the tank while maintaining stable pressure inside. A pressure gauge inside the tank monitors pressure changes in real time; if the pressure is abnormal, the gas delivery mechanism 6 automatically adjusts the aeration rate. A pH probe monitors the acidity and alkalinity of the fermentation broth; when the pH value deviates from the set range, an acid-base regulator is added to maintain a suitable pH. A biosensor analyzer monitors key parameters such as AKK bacteria concentration, substrate concentration, and metabolite concentration in real time, providing data support for precise control of the fermentation process.

[0041] After the culture medium enters the anaerobic fermenter 2, the stirring motor 81 drives the stirring shaft 82 and stirring rod 83 to rotate, ensuring thorough mixing of the mother liquor with each culture medium. This guarantees uniform distribution of nutrients, promotes full contact between AKK bacteria and nutrients, enhances the mass transfer process, and prevents solid sedimentation and equipment scaling. Throughout the cultivation process, the biosensor analyzer automatically or manually adjusts parameters such as the feed rate of the mother liquor tank 3 and the culture medium preparation tank 4, the aeration rate of the gas delivery mechanism 6, and the stirring speed based on real-time monitoring data and preset cultivation parameter ranges, achieving dynamic optimization of AKK bacteria cultivation conditions.

[0042] When the AKK bacteria reach the expected concentration or the fermentation stage is completed, the discharge channel 7 at the bottom of the anaerobic fermenter 2 opens. Under the control of the closed opening and closing door 71, the cultured fermentation broth is discharged quantitatively as needed for subsequent separation, purification and other processing steps. At the same time, new mother liquor and culture medium can continue to be introduced to achieve efficient and continuous culture of AKK bacteria.

[0043] In this embodiment, each of the culture medium supply mechanisms 5 includes a peristaltic pump 51, an inlet pipe 52, and an outlet pipe 53. The peristaltic pump 51 is connected to the side wall of the anaerobic fermenter 2. The two ends of the outlet pipe 53 are respectively connected to the peristaltic pump 51 and the anaerobic fermenter 2. The inlet pipe 52 is respectively connected to the peristaltic pump 51 and the anaerobic fermenter 2 or the mother liquor tank 3.

[0044] During the cultivation process, the peristaltic pump 51 drives the various culture media to be drawn from the culture medium preparation tank 4 through the feed pipe, and then transported to the anaerobic fermenter 2 through the discharge pipe. The peristaltic pump 51 also drives the mother liquor required for cultivation to be drawn from the mother liquor tank 3 through the feed pipe, and then transported to the anaerobic fermenter 2 through the discharge pipe, thereby realizing the delivery of the Chinese medicine culture medium.

[0045] The peristaltic pump 51 is model number: Watson-Marlow 505S series.

[0046] In this embodiment, a flow meter is installed on the outlet pipe 53. The flow meter model is KROHNE OPTIFLUX4000C series. The flow meter can monitor in real time the amount of culture medium delivered to the anaerobic fermenter 2 through the outlet pipe 53, so that the amount of each culture medium in the anaerobic fermenter 2 is the optimal amount required for cultivation.

[0047] In this embodiment, the bottom of the anaerobic fermenter 2 is provided with a motor base 8, the motor base 8 is provided with a stirring motor 81, the main shaft of the stirring motor 81 is provided with a vertically arranged stirring shaft 82, the stirring shaft 82 extends into the interior of the anaerobic fermenter 2, and the stirring shaft 82 is provided with a plurality of stirring rods 83.

[0048] After the various culture media are delivered into the anaerobic fermenter 2, the stirring motor 81 drives the stirring shaft 82, which in turn drives several stirring rods 83 to rotate. This mixes the mother liquor and various culture media in the anaerobic fermenter 2, ensuring that the nutrients in the various culture media, such as carbon sources, nitrogen sources, vitamins, and minerals, are fully and evenly mixed in the mother liquor. This avoids situations where the concentration of nutrients is too high or too low in certain areas, providing a balanced nutritional environment for anaerobic microorganisms, which is beneficial to their growth and metabolism. It also ensures that the anaerobic microorganisms are evenly distributed in the fermenter, allowing them to fully contact the nutrients, improving the utilization rate of nutrients by the microorganisms, promoting the synchronicity of the fermentation process, and avoiding uneven growth of microorganisms due to uneven distribution of nutrients, which would affect the fermentation effect.

[0049] Accelerating the transfer of nutrients from the bulk liquid phase to the surface of microbial cells allows substrates to be absorbed and utilized by microorganisms more quickly. It also helps microbial metabolites diffuse from the cell surface to the bulk liquid phase, thereby improving fermentation efficiency.

[0050] In this embodiment, the anaerobic fermenter 2 is equipped with a pressure gauge, a pH probe, and a biosensor analyzer. The pressure gauge is a Rosemount 3051S series; the pH probe is a Mettler Toledo InPro3250 series; and the biosensor analyzer is a Siemens Bioprofile series.

[0051] The pressure gauge can monitor the pressure inside the anaerobic digester 2 in real time, reflecting the dynamic balance of gas production and consumption during fermentation. For example, during anaerobic fermentation, microorganisms decompose organic matter, producing gases such as carbon dioxide and methane, which causes the pressure inside the tank to rise. Through the pressure gauge, operators can intuitively understand the pressure values ​​and determine whether fermentation is proceeding normally.

[0052] A pH probe can monitor the pH changes of the fermentation broth in real time during the fermentation process. Different anaerobic fermentation processes, such as alcoholic fermentation and lactic acid fermentation, have their own suitable pH ranges. For example, alcoholic fermentation is generally suitable for an acidic environment with a pH of 4.0-5.8. Through a pH probe, operators can monitor the acidity or alkalinity of the fermentation broth at any time to promptly detect any abnormal changes.

[0053] Biosensor analyzers can monitor various biological parameters during the fermentation process in real time, such as microbial concentration, substrate concentration, and metabolite concentration. For example, in the process of anaerobic fermentation to produce biogas, biosensor analyzers can monitor the concentration of methanogens, organic substrates, and metabolites such as methane in the fermentation broth, providing operators with comprehensive fermentation information.

[0054] The biosensor analyzer enables automated control: Connected to the control system of anaerobic fermenter 2, the biosensor analyzer allows for automated control of the fermentation process. When the parameters of the culture medium in anaerobic fermenter 2 deviate from the set values, the control system automatically adjusts relevant parameters, such as automatically adding substrate and adjusting pH, ensuring the fermentation process remains optimal. This not only improves production efficiency and product quality stability but also reduces errors and labor intensity caused by manual operation.

[0055] In this embodiment, the gas delivery mechanism 6 is equipped with a mass flow meter. AKK bacteria cultivation requires a specific gaseous environment, such as a mixture of 5% H2, 10% CO2, and 85% N2. The gas mixing device can precisely control the proportions of various gases, accurately measuring and adjusting the gas flow rate through instruments such as the mass flow meter to ensure a stable gas mixture composition that meets the growth requirements of AKK bacteria. The mass flow meter model is MicroMotionG series.

[0056] In this embodiment, the anaerobic incubator 1 is provided with a gas exchange machine 11 and a circulating fan 12 on its side wall. The gas exchange machine 11 and the circulating fan 12 are respectively Edwards GV600 series and EBM-Papst AxiFans series.

[0057] When culturing AKK bacteria, the gas replacement unit 11 can quickly replace the air in the incubator with anaerobic gases such as nitrogen and hydrogen. This rapidly reduces the oxygen content, achieving the low-oxygen or anaerobic conditions required for anaerobic microbial growth, providing a suitable living environment for strict anaerobic bacteria like AKK. It can precisely control the type, proportion, and flow rate of the input gases, ensuring a stable anaerobic environment within the incubator. Through precise control, the oxygen concentration can be kept at extremely low levels while ensuring that other gas components meet the requirements for microbial growth, thus improving the success rate of cultivation and the quality of microbial growth. During cultivation, the metabolic activities of microorganisms may alter the gas composition within the incubator. The gas replacement unit 11 facilitates periodic or as-needed gas replacement, removing metabolically produced gases such as carbon dioxide and hydrogen, and replenishing consumed nutrients to maintain a stable cultivation environment.

[0058] During the cultivation of AKK bacteria, the circulating fan 12 ensures thorough mixing and uniform distribution of the gas within the incubator. This guarantees consistent environmental conditions such as gas composition, temperature, and humidity across all areas of the incubator, preventing localized excessively high or low oxygen concentrations or uneven temperatures. This promotes uniform microbial growth and metabolism, improving the consistency and reproducibility of the culture. It also facilitates uniform heat transfer within the incubator, resulting in more stable temperatures. Microbial growth is temperature-sensitive; a uniform temperature distribution prevents localized overheating or cooling that could negatively impact growth and metabolism, providing a stable thermal environment that helps maintain physiological activity and growth rate. The circulating fan 12 also drives gas flow, indirectly promoting the uniform distribution and transfer of nutrients in the culture medium. This allows microorganisms to more fully contact and absorb nutrients, which is beneficial for their growth and reproduction. It also facilitates the diffusion of metabolic products, preventing the accumulation of localized metabolic waste that could inhibit microbial growth.

[0059] An oxygen sensor and a carbon dioxide sensor are installed on the internal side wall of the anaerobic incubator 1. The gas replacement machine 11 and the circulating fan 12 are electrically connected to the oxygen sensor and the carbon dioxide sensor. The oxygen sensor is model LOX-02, and the carbon dioxide sensor is model ExplorerIR-M.

[0060] The oxygen sensor installed in the anaerobic incubator 1 serves to: accurately monitor the oxygen content in the incubator in real time, and intuitively display the oxygen concentration value, allowing operators to accurately understand whether the incubator has reached and maintained an anaerobic state, providing an anaerobic or low-oxygen growth environment for anaerobic microorganisms such as AKK bacteria; and to feed back the monitored oxygen concentration information to the control system. When abnormal fluctuations occur in the oxygen concentration, such as air entering due to equipment sealing problems leading to an increase in oxygen concentration, the control system can take timely measures based on the feedback signal, such as activating the gas replacement device, to maintain a stable anaerobic environment, ensuring the normal growth of microorganisms and the accuracy of experimental results.

[0061] The function of the carbon dioxide sensor installed in the anaerobic incubator 1 is as follows: Carbon dioxide is a cellular metabolic product and an essential component for cell growth, and it is closely related to the pH value of the culture medium. The sensor monitors the carbon dioxide concentration within the incubator and transmits this information to the control system, which then controls the input of carbon dioxide to maintain the pH value of the culture medium within a suitable range for microbial growth, providing a stable acid-base environment for microorganisms. Different microorganisms have different requirements for carbon dioxide concentration; by precisely controlling the carbon dioxide content within the incubator through the carbon dioxide sensor, the growth needs of specific microorganisms can be met, culture conditions can be optimized, and the growth rate and activity of microorganisms can be improved.

[0062] In this embodiment, the discharge channel 7 is provided with a closed opening and closing door 71.

[0063] Controlling material flow: By opening and closing the door 71, the timing and flow rate of the culture medium discharged from the anaerobic fermenter 2 into the subsequent process can be precisely controlled, ensuring that the material is delivered quantitatively according to production needs, which helps to ensure the stability and consistency of product quality.

[0064] Preventing contamination: When not discharging, the closed opening and closing door 71 can effectively prevent external air, dust, microorganisms, etc. from entering the discharge channel 7 and anaerobic fermenter 2, avoiding contamination of the culture medium. This is crucial for maintaining the sterility of the culture medium, especially for some culture media that are sensitive to microbial contamination.

[0065] Maintaining a clean environment: Preventing culture medium from leaking into the surrounding environment during the discharge process, avoiding pollution of the production workshop floor, equipment, etc., helps to maintain a clean and hygienic production environment, and complies with relevant standards and requirements such as GMP (Good Manufacturing Practice for Pharmaceuticals).

[0066] In this embodiment, a sealing door 13 is provided on the side wall of the anaerobic incubator 1;

[0067] Maintaining an anaerobic environment: The sealed door 13 effectively prevents outside air from entering the incubator, thus maintaining the anaerobic state inside. This is crucial for the growth and cultivation of anaerobic bacteria, such as AKK bacteria, as they are extremely sensitive to oxygen; even a small amount of oxygen intrusion can affect their growth or even lead to their death.

[0068] Preventing contamination: A good seal can prevent contaminants such as bacteria and dust from the external environment from entering the incubator, avoiding contamination of the cultured microorganisms, ensuring the purity of the culture environment, and providing stable and suitable growth conditions for the microorganisms.

[0069] In this embodiment, the three culture medium preparation tanks 4 are liquid storage tanks containing traditional Chinese medicine culture medium.

[0070] The culture medium for traditional Chinese medicine can be the one published in Chinese Patent Publication No. CN113082145B.

[0071] The above description is merely an embodiment of this utility model and is not intended to limit the scope of this utility model. Various modifications and variations can be made to this utility model by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principle of this utility model should be included within the scope of the claims of this utility model.

Claims

1. A high-efficiency continuous culture device for AKK bacteria, characterized in that: The anaerobic fermenter includes an anaerobic incubator (1) and an anaerobic fermenter (2), the anaerobic fermenter (2) being located inside the anaerobic incubator (1); a mother liquor tank (3) and three culture medium preparation tanks (4) are provided on the side of the anaerobic fermenter (2), the mother liquor tank (3) and the three culture medium preparation tanks (4) are provided with culture medium supply mechanisms (5) on the anaerobic fermenter (2), the mother liquor tank (3) and the three culture medium preparation tanks (4) are connected to the anaerobic fermenter (2) through the culture medium supply mechanism (5), the top of the anaerobic fermenter (2) is provided with a gas conveying mechanism (6) and the gas conveying mechanism (6) is connected to the anaerobic fermenter (2), and the bottom of the anaerobic fermenter (2) is provided with a discharge channel (7).

2. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: Each of the culture medium supply mechanisms (5) includes a peristaltic pump (51), an inlet pipe (52), and an outlet pipe (53). The peristaltic pump (51) is connected to the side wall of the anaerobic fermenter (2). The two ends of the outlet pipe (53) are connected to the peristaltic pump (51) and the anaerobic fermenter (2), respectively. The inlet pipe (52) is connected to the peristaltic pump (51) and the anaerobic fermenter (2) or the mother liquor tank (3), respectively.

3. The high-efficiency continuous culture device for AKK bacteria according to claim 2, characterized in that: A flow meter is installed on the liquid outlet pipe (53).

4. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The bottom of the anaerobic fermenter (2) is provided with a motor base (8), and a stirring motor (81) is provided on the motor base (8). A vertically arranged stirring shaft (82) is provided on the main shaft of the stirring motor (81). The stirring shaft (82) extends into the interior of the anaerobic fermenter (2), and a number of stirring rods (83) are provided on the stirring shaft (82).

5. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The anaerobic fermenter (2) is equipped with a pressure gauge, a pH probe and a biosensor analyzer.

6. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The gas delivery mechanism (6) is equipped with a mass flow meter.

7. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The anaerobic incubator (1) is provided with a gas exchange machine (11) and a circulating fan (12) on its side wall. An oxygen sensor and a carbon dioxide sensor are provided on the inner side wall of the anaerobic incubator (1). The gas exchange machine (11) and the circulating fan (12) are electrically connected to the oxygen sensor and the carbon dioxide sensor.

8. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The discharge channel (7) is equipped with a closed opening and closing door (71).

9. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The anaerobic incubator (1) has a sealed door (13) on its side wall.

10. The high-efficiency continuous culture device for AKK bacteria according to claim 1, characterized in that: The three culture medium preparation tanks (4) are liquid storage tanks containing traditional Chinese medicine culture medium.

Images