Section staining rack for immunohistochemical experiment

Abstract

The utility model discloses a section staining rack for an immunohistochemical experiment, which comprises a base, a vertical shaft is vertically and upwards arranged in the middle of the base, a sleeve is sleeved outside the vertical shaft, an upper tray is arranged at the upper part of the sleeve, a lower tray is arranged at the lower part of the sleeve, and the upper tray comprises an upper inner ring disc and an upper outer ring disc. The lower tray comprises a lower inner ring disc and a lower outer ring disc, clamping grooves are correspondingly formed in the outer side of the upper inner ring disc, the inner side of the upper outer ring disc, the outer side of the lower inner ring disc and the inner side of the lower outer ring disc around the center of the sleeve in a radial mode, and glass slides with wax sealing slices are inserted into the clamping grooves. The utility model aims to provide the section staining rack for the immunohistochemical experiment, which can be used for more sufficiently staining sections by rotating the staining rack, has a good staining effect, is beneficial to clear observation under a microscope and avoids experimental errors.

Description

Technical Field

[0001] This utility model relates to the technical field of equipment for immunological experiments, specifically a slide staining rack for immunohistochemistry experiments. Background Technology

[0002] Immunohistochemical experiments typically involve multiple steps, including sample fixation, sectioning, antigen retrieval, antibody incubation, and staining. Specifically, biological cell tissue of a certain size is embedded in paraffin blocks, sliced ​​into thin sections using a microtome, stained, and then observed under a microscope for further analysis of the cell and tissue structure. In section preparation, a portion of the cell tissue is treated with various chemicals and embedding methods to fix and harden it. It is then sliced ​​into thin sections using a microtome, adhered to a glass slide, and stained with various colors for observation under a microscope. Currently, biological cell sections are prepared by placing a glass slide on a staining rack, which is mostly square. During staining, the rack is placed in a square box containing staining solution, and the process is repeated several times to complete the staining. The square staining rack is paired with a square staining box. Due to space limitations, the rack can only be moved vertically and cannot rotate horizontally, resulting in poor staining effects and hindering clear observation under a microscope, thus affecting the experimental results. Therefore, there is a need to develop a staining rack for immunohistochemistry experiments that can make the slides more thoroughly stained by rotating the staining rack, resulting in better staining effects and making it easier to observe under a microscope, thus avoiding experimental errors. Utility Model Content

[0003] The purpose of this invention is to provide a slide staining rack for immunohistochemistry experiments that allows for more thorough staining of slides by rotating the staining rack, resulting in better staining effects and easier observation under a microscope, thus avoiding experimental errors.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a slide staining rack for immunohistochemistry experiments, comprising a base, a vertical axis vertically upwardly arranged in the middle of the base, a sleeve fitted outside the vertical axis, an upper tray at the upper part of the sleeve, and a lower tray at the lower part. The upper tray includes an upper inner ring disk and an upper outer ring disk, and the lower tray includes a lower inner ring disk and a lower outer ring disk. The outer side of the upper inner ring disk and the inner side of the upper outer ring disk, as well as the outer side of the lower inner ring disk and the inner side of the lower outer ring disk, are respectively provided with slots radially around the center of the sleeve. Glass slides with sealed wax sections are inserted into the slots.

[0005] Furthermore, the upper pallet, lower pallet, and sleeve are connected by circumferentially arranged vertical plates.

[0006] Furthermore, the base, the upper outer ring, and the lower outer ring are shaped as either a circle or a regular polygon.

[0007] Furthermore, the top of the vertical shaft is threaded, and a nut is screwed onto the thread. There is a gap between the top of the sleeve and the bottom of the nut, so that the sleeve can rotate around the vertical shaft.

[0008] Furthermore, a handle is provided at the top of the vertical axis.

[0009] The beneficial effects of this invention are as follows: This device inserts a glass slide with cell sections into a slot between the upper and lower trays. Since the upper and lower trays are connected to the sleeve via a vertical plate, the sleeve can rotate around the vertical axis. Thus, when staining, the staining rack is placed in the staining tank, which can not only be lifted up and down, but also rotated within the staining tank. This allows the slides to be immersed in the staining axially and radially, resulting in more thorough and uniform staining, better staining effect, and easier observation of cell tissue structure under a microscope, avoiding experimental errors. At the same time, this device is designed as a detachable structure, which is easy to clean after disassembly and easy to store after assembly. Attached Figure Description

[0010] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0011] Figure 2 for Figure 1 Sectional view of AA in the middle;

[0012] In the diagram: 1-base, 2-lower tray, 21-lower inner ring plate, 22-lower outer ring plate, 3-glass slide, 4-upper tray, 41-upper inner ring plate, 42-upper outer ring plate, 5-sleeve, 6-vertical shaft, 7-nut, 8-handle, 9-vertical plate, 10-slot. Detailed Implementation

[0013] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0014] Please see Figure 1-2This utility model provides a technical solution: a slide staining rack for immunohistochemistry experiments, including a base 1, a vertical shaft 6 vertically upwardly arranged in the middle of the base 1, a sleeve 5 fitted outside the vertical shaft 6, an upper tray 4 arranged at the upper part of the sleeve 5, and a lower tray 2 arranged at the lower part. The upper tray 4 includes an upper inner ring disk 41 and an upper outer ring disk 42, and the lower tray 2 includes a lower inner ring disk 21 and a lower outer ring disk 22. The outer side of the upper inner ring disk 41 and the inner side of the upper outer ring disk 42, as well as the outer side of the lower inner ring disk 21 and the inner side of the lower outer ring disk 22, are respectively provided with slots 10 radially around the center of the sleeve 5. A glass slide 3 with a sealed wax section is inserted into the slot 10.

[0015] The upper pallet 4, lower pallet 2, and sleeve 5 are connected by circumferentially arranged upright plates 9. The upright plates 9 serve as supports and connections.

[0016] The base 1, the upper outer ring 42, and the lower outer ring 22 are shaped as either a circle or a regular polygon.

[0017] The top of the vertical shaft 6 is threaded, and a nut 7 is screwed onto the thread. There is a gap between the top of the sleeve 5 and the bottom of the nut 7, so that the sleeve 5 can rotate around the vertical shaft 6.

[0018] A handle 8 is provided at the top of the vertical axis 6. The entire dyeing rack can be lifted by using the handle 8.

[0019] In use, the prepared biological cell tissue sections are first inserted longitudinally into the slots 10, which consist of the corresponding upper inner ring disk 41, upper outer ring disk 42, lower inner ring disk 21, and lower outer ring disk 22, via a glass slide 3. The glass slide 3 is then fixed in place by the slots 10. Since the upper tray 4 and lower tray 2 are connected to the sleeve 5 via the upright plate 9, the sleeve 5 can rotate around the center of the vertical axis 6. Thus, during staining, the staining rack is placed in the staining tank, which can not only be lifted up and down, but also rotated within the staining tank. This allows the sections to be immersed in the staining axially and radially, resulting in more thorough and uniform staining, better staining effect, and clearer observation of cell tissue structure under a microscope, avoiding experimental errors. At the same time, this device is designed as a detachable structure, which is easy to clean after disassembly and easy to store after assembly.

Claims

1. A slide staining rack for immunohistochemistry experiments, comprising a base (1), characterized in that: A vertical shaft (6) is vertically installed in the middle of the base (1). A sleeve (5) is fitted on the outside of the vertical shaft (6). An upper tray (4) is installed on the upper part of the sleeve (5), and a lower tray (2) is installed on the lower part. The upper tray (4) includes an upper inner ring plate (41) and an upper outer ring plate (42). The lower tray (2) includes a lower inner ring plate (21) and a lower outer ring plate (22). The outer side of the upper inner ring plate (41) and the inner side of the upper outer ring plate (42), as well as the outer side of the lower inner ring plate (21) and the inner side of the lower outer ring plate (22), are respectively arranged in a radial pattern around the center of the sleeve (5) with corresponding slots (10). A glass slide (3) with a sealed wax section is inserted into the slot (10).

2. The slide staining rack for immunohistochemistry experiments according to claim 1, characterized in that: The upper pallet (4), lower pallet (2) and sleeve (5) are connected by circumferentially arranged vertical plates (9).

3. The slide staining rack for immunohistochemistry experiments according to claim 1, characterized in that: The base (1), the upper outer ring (42), and the lower outer ring (22) are shaped as either a circle or a regular polygon.

4. The slide staining rack for immunohistochemistry experiments according to claim 1, characterized in that: The top of the vertical shaft (6) is threaded, and a nut (7) is screwed onto the thread. There is a gap between the top of the sleeve (5) and the bottom of the nut (7) so that the sleeve (5) can rotate around the vertical shaft (6).

5. The slide staining rack for immunohistochemistry experiments according to claim 1, characterized in that: A handle (8) is provided at the top of the vertical axis (6).

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