Fungus fermentation culture device

Abstract

The fungus fermentation culture device comprises a box body, an outer circulation assembly and an inner circulation assembly, and an inner cavity of the box body is used for containing a fermentation container; comprising a gas inlet channel and an outer circulation channel, the gas inlet channel is used for guiding gas into the box body, and the outer circulation channel communicates with the gas inlet channel and an inner cavity of the box body; comprising an internal circulation part located in the box body, and the internal circulation part is used for driving gas in the box body to flow. The air inlet channel guides compressed air into the box body, and the compressed air flows in the inner cavity of the box body under the action of the internal circulation component to provide oxygen for the fermentation process of microorganisms so as to form internal circulation of air; part of air in the inner cavity of the box body returns to the air inlet channel along the outer circulation channel and enters the box body again along the air inlet channel, and air outer circulation is formed. Under the combined action of internal circulation and external circulation, the ventilation capacity of the box body is improved, sufficient oxygen is ensured to be obtained by microorganisms, and the fermentation efficiency is improved.

Description

Technical Field

[0001] This application relates to the field of fermentation equipment technology, and in particular to a fungal fermentation culture device. Background Technology

[0002] Fungal proteins are usually cultured using liquid shallow fermentation. Liquid shallow fermentation involves placing a thin layer of culture medium in a shallow dish, inoculating it, and then performing surface culture. Microorganisms form a thin film on the liquid surface. This method is suitable for some fast-reproducing aerobic microorganisms.

[0003] Aseptic conditions are crucial during liquid shallow fermentation, and to prevent contamination, the fermentation process is typically conducted in a sealed fermentation chamber. However, current fermentation chambers often have poor ventilation, making it difficult to ensure sufficient oxygen supply to microorganisms, leading to decreased product formation efficiency. Furthermore, liquid shallow fermentation involves environmental monitoring equipment, fermentation devices, and culture medium feeding, requiring the movement of large equipment into and out of the fermentation chamber. The rigid structure of the fermentation chamber makes the equipment susceptible to damage and is inconvenient to disassemble and replace. Before samples enter the fermentation device for further fermentation, they often face inconsistencies between the internal and external gas environments, necessitating adjustments before entry. Additionally, for fermentation processes with large internal spaces and numerous instruments, current fermentation devices lack the capability for multiple personnel to simultaneously operate the equipment and culture medium from the outside, hindering timely and synchronized control of multiple parameters during fermentation. The culture medium feeding process also struggles to be adjusted synchronously during the intermediate stages of fermentation. Utility Model Content

[0004] This application aims to address at least one of the technical problems existing in the prior art. To this end, this application proposes a fungal fermentation culture device that ensures gas circulation within the chamber through internal and external circulation components. The chamber utilizes a flexible and soft membrane structure, preventing damage to large equipment entering the fermentation culture device; simultaneously, the membrane is easily removable, allowing for the construction of different fermentation culture devices by laying membranes of varying sizes. Furthermore, the fermentation culture device incorporates a transfer buffer window to adjust the gas environment of samples entering the fermentation culture device to maintain consistency with the internal gas environment. The side walls of the chamber feature multiple glove-type operating windows, facilitating operation of the instruments inside the fermentation culture device by multiple people using gloves, and also allowing for sample manipulation during the fermentation process.

[0005] According to a first aspect embodiment of the present application, the fungal fermentation culture apparatus includes a box, an external circulation component, and an internal circulation component. The inner cavity of the box is used to place a fermentation container. It includes an air inlet channel and an external circulation channel. The air inlet channel is used to introduce gas into the box, and the external circulation channel connects the air inlet channel and the inner cavity of the box. It also includes an internal circulation component located within the box, which is used to drive the gas flow within the box.

[0006] The fungal fermentation culture device according to the embodiments of this application has at least the following beneficial effects: the air inlet channel introduces compressed air into the chamber, and under the action of the internal circulation component, the compressed air flows in the inner cavity of the chamber, providing oxygen for the fermentation process of microorganisms and forming an internal air circulation; some of the gas in the inner cavity of the chamber returns to the air inlet channel along the external circulation channel and re-enters the chamber along the air inlet channel, forming an external air circulation; under the combined action of internal and external circulation, the ventilation capacity of the chamber is improved, ensuring that microorganisms obtain sufficient oxygen and improving fermentation efficiency.

[0007] According to some embodiments of this application, the connection positions of the air intake channel and the housing, and the connection positions of the external circulation channel and the housing, are located on opposite sides of the housing.

[0008] According to some embodiments of this application, the housing is made of a flexible membrane structure.

[0009] According to some embodiments of this application, the fermentation culture apparatus further includes a buffer device, the chamber is provided with a transfer buffer window, and the buffer device is connected to the transfer buffer window.

[0010] According to some embodiments of this application, the fermentation culture apparatus further includes a humidity regulating component located within the chamber, the humidity regulating component being used to adjust the ambient humidity within the chamber.

[0011] According to some embodiments of this application, the humidity regulating assembly includes a humidity regulating device and a dispersing component connected to each other, and the steam generated by the humidity regulating device is evenly distributed in the inner cavity of the box through the dispersing component.

[0012] According to some embodiments of this application, the enclosure further includes a door structure, which is closable within the enclosure.

[0013] According to some embodiments of this application, the enclosure is provided with an operation window, and the operation window is provided with operation components, through which the operator performs operations inside the enclosure.

[0014] According to some embodiments of this application, the operating component includes a flexible extension member and a contouring member, the contouring member being located at the end of the extension member.

[0015] According to some embodiments of this application, the fermentation culture device further includes a support frame, and the box body is provided with a connecting structure, through which the box body is detachably connected to the support frame.

[0016] Additional aspects and advantages of this application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of this application. Attached Figure Description

[0017] The accompanying drawings are used to provide a further understanding of the technical solutions disclosed in this application and form part of the specification. They are used together with the embodiments disclosed in this application to explain the technical solutions of this application and do not constitute a limitation on the technical solutions disclosed in this application.

[0018] Figure 1 This is a schematic diagram of the structure of the fungal fermentation culture device according to an embodiment of this application;

[0019] Figure 2 This is a schematic diagram of the box and its internal structure in the fungal fermentation culture device according to an embodiment of this application;

[0020] Figure 3 This is a schematic diagram of the structure of the box and external circulation components in the fungal fermentation culture device of this application embodiment.

[0021] Figure label:

[0022] Box body 101; Fermentation container 102; Bottom plate 103; Door structure 104;

[0023] 201 bracket; 202 connecting structure;

[0024] Intake passage 301; External circulation passage 302; Internal circulation component 303; Exhaust passage 304;

[0025] Buffer device 401; Transfer buffer window 402;

[0026] Humidity regulating device 501; Dispersion component 502;

[0027] Extension component 601; contouring component 602; operation window 603. Detailed Implementation

[0028] The embodiments of this application are described in detail below. Examples of these embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar elements or elements having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain this application, and should not be construed as limiting this application.

[0029] In the description of this application, it should be understood that the orientation descriptions, such as up, down, front, back, left, right, etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0030] In the description of this application, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0031] In the description of this application, unless otherwise expressly defined, terms such as "setup," "installation," and "connection" should be interpreted broadly, and those skilled in the art can reasonably determine the specific meaning of the above terms in this application in conjunction with the specific content of the technical solution.

[0032] In the description of this application, the terms "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0033] This application provides a fungal fermentation culture device, which includes a housing 101, an external circulation component, and an internal circulation component. The housing 101 has an inner cavity to provide space for the fermentation process of microbial samples. It is understood that the microbial samples are contained in a fermentation container 102, and the fermentation container 102 is placed within the inner cavity of the housing 101.

[0034] Furthermore, the external circulation component is used to introduce gas into the housing 101, specifically, the gas here is compressed air. At the same time, the external circulation component is also used to exhaust gas from the housing 101. The exhausted gas is filtered and then reintroduced into the housing 101, forming an external circulation process to ensure gas flow.

[0035] In addition, the internal circulation component is used to drive the gas flow inside the chamber 101, thereby forming an internal gas circulation process. This ensures gas flow while providing sufficient oxygen for the microbial sample, thus improving the efficiency of the fermentation process.

[0036] In some examples, such as Figure 1 and Figure 2 As shown, the enclosure 101 is made of a flexible membrane structure, which defines the interior cavity of the enclosure 101. Specifically, the flexible membrane structure is a plastic membrane made of vinyl. It is understood that the flexible membrane structure is elastic and flexible, facilitating the entry and exit of large equipment when it is installed inside the enclosure 101 or disassembled, and reducing the risk of damage to the equipment.

[0037] The enclosure 101 has a rigid base plate 103 inside, which is located at the bottom of the enclosure 101. Various devices inside the enclosure 101 are placed on the base plate 103 to ensure stability.

[0038] Furthermore, the transparent vinyl material gives the box 101 good light transmittance, making it easy for staff to observe the fermentation process inside the box 101 from the outside.

[0039] Meanwhile, the housing 101 has good sealing properties, which makes it easy for this device to meet the environmental requirements of the shallow fermentation process of microbial samples and avoid contamination by other microorganisms.

[0040] In some examples, the enclosure 101 is provided with an entrance and exit, and a door structure 104 is provided at the entrance and exit. When the door structure 104 opens the entrance and exit, the relevant equipment is inserted into the enclosure 101 along the entrance and exit, or removed from the enclosure 101 along the entrance and exit; when the door structure 104 closes the entrance and exit, the enclosure 101 is closed to prevent contamination of microbial samples.

[0041] In some examples, such as Figure 1 As shown, since the box 101 is flexible and difficult to maintain a fixed shape, the fermentation culture device also includes a support 201, which is used to support the box 101.

[0042] The main body of the bracket 201 is located on opposite sides of the housing 101, and the shape of the main body of the bracket 201 can be set according to actual needs. Specifically, the main body of the bracket 201 in this device is made of bent rods, and the middle of the rods of the main body of the bracket 201 is raised, thus having a certain height.

[0043] Furthermore, the main body of the brackets 201 on both sides is combined into a whole through the connecting part of the brackets 201. The connecting part of the brackets 201 is also made of rods and is kept horizontal at a certain height.

[0044] Meanwhile, the housing 101 is provided with a connecting structure 202, through which the housing 101 is detachably connected to the bracket 201. Specifically, the connecting structure 202 is a flexible component with its two sides fixed to the outside of the housing 101, and the connecting part of the bracket 201 passes between the connecting structure 202 and the housing 101, so that the housing 101 is hung on the bracket 201.

[0045] In some examples, the shallow fermentation process of microbial samples needs to meet specific culture environment requirements. Specifically, the temperature inside chamber 101 needs to be maintained between 20°C and 32°C, the humidity inside chamber 101 needs to be maintained between 50% and 90%, and the carbon dioxide concentration inside chamber 101 needs to be maintained between 0% and 10%.

[0046] Furthermore, the enclosure 101 is equipped with temperature, humidity and carbon dioxide detection probes, which are connected to control valves for adjusting the corresponding parameters, thereby adjusting the environmental parameters inside the enclosure 101 in real time.

[0047] In some examples, such as Figure 3 As shown, the external circulation assembly includes an intake channel 301 and an external circulation channel 302, and the corresponding pipes define the intake channel 301 and the external circulation channel 302 through the inner cavity.

[0048] The air intake channel 301 is used to introduce compressed air into the inner cavity of the housing 101. The air intake channel 301 intakes air on one side of the housing 101 and connects to the inner cavity of the housing 101 on the other side.

[0049] Furthermore, the external circulation channel 302 is used to exhaust air from the inner cavity of the chamber 101 and reintroduce the exhausted air into the air intake channel 301, thereby achieving external air circulation and ensuring air flow. It is understood that air flow allows oxygen to continuously reach the fermentation container 102, ensuring that the microbial samples receive sufficient oxygen.

[0050] Specifically, the external circulation channel 302 is connected to the intake channel 301 on one side and to the housing 101 on the other. The positions where the external circulation channel 302 connects to the intake channel 301 and to the housing 101 are both close to the intake end of the intake channel 301, that is, the intake ends of the external circulation channel 302 and the intake channel 301 are on the same side of the housing 101.

[0051] In some examples, the intake passage 301 has a filter membrane. Compressed air enters the housing 101 after being filtered by the filter membrane along the intake passage 301. Some of the air in the housing 101 is discharged from the housing 101 along the external circulation passage 302 and re-enters the intake passage 301. The discharged air is then filtered by the filter membrane and re-enters the housing 101.

[0052] Furthermore, fans can be installed in each channel to power the air and ensure that the air flows in a preset direction.

[0053] In some examples, the internal circulation component includes an internal circulation part 303 located within the housing 101. Specifically, the internal circulation part 303 employs a fan to drive the airflow within the housing 101, thereby achieving internal air circulation, which further ensures air flow.

[0054] The chamber 101 is equipped with a shelf, on which the internal circulation component 303 and the fermentation container 102 are placed. The chamber 101 also has an exhaust channel 304, which is used to expel carbon dioxide from the chamber 101, ensuring sufficient oxygen levels within the chamber.

[0055] In some examples, such as Figure 1 As shown, the fermentation culture device also includes a buffer device 401. The box body 101 is provided with a transfer buffer window 402. The buffer device 401 is connected to the transfer buffer window 402. The fermentation container 102 containing the microbial sample is placed into the interior of the box body 101 through the transfer buffer window 402 via the buffer device 401.

[0056] The buffer device 401 has an inner cavity and a buffer door that connects to the inner cavity. The fermentation container 102 first enters the inner cavity of the buffer device 401, so that the microbial sample before entering the box 101 is pre-adjusted to the same gas environment as the box 101, thus preventing external pollutants from entering the box 101.

[0057] In some examples, such as Figure 2 As shown, the fermentation culture device also includes a humidity control component located inside the chamber 101, which is used to adjust the ambient humidity inside the chamber 101.

[0058] The humidity control component includes a humidity control device 501 and a dispersing component 502, the dispersing component 502 being a tubular structure of a certain length. The humidity control device 501 and the dispersing component 502 are connected, and steam is delivered to the dispersing component 502.

[0059] Furthermore, the dispersing component 502 first extends to the top of the inner cavity of the housing 101, then extends horizontally, and finally extends to the bottom of the inner cavity of the housing 101. At the same time, the surface of the dispersing component 502 has several through holes, and the distribution of the through holes can be set according to actual needs to ensure that the steam is evenly distributed in the inner cavity of the housing 101.

[0060] In some examples, such as Figure 1As shown, the housing 101 is provided with several operation windows 603, and each operation window 603 is equipped with an operation component. The staff can perform various operations inside the housing 101 through the operation component.

[0061] Furthermore, the operating component includes a flexible extension member 601 and a contouring member 602. The contouring member 602 is located at the end of the extension member 601. Specifically, the contouring member 602 is shaped like a human hand, making it easy to slip over the operator's hand. Because the extension member 601 is flexible, the operator's arm can extend the extension member 601 and the contouring member 602 into the interior of the housing 101, allowing operation to be performed inside the housing 101 while ensuring the housing 101 is sealed.

[0062] Specifically, the operation window 603 and operation components are set in four groups, allowing multiple people to operate simultaneously or operate the relevant internal instruments from different locations.

[0063] In actual implementation, the fermentation container 102 is placed inside the chamber 101 after being treated by the buffer device 401. The internal environment of the chamber 101 is adjusted in real time according to the needs of the microbial samples, and the staff performs the required operations through the operating components. The internal circulation component realizes internal air circulation inside the chamber 101, and the external circulation component realizes external air circulation outside the chamber 101. Under the combined effect of internal and external circulation, the microbial samples can obtain sufficient oxygen, ensuring the efficient progress of the fermentation process.

[0064] In some alternative embodiments, the functions / operations mentioned in the block diagrams may not occur in the order shown in the operation diagrams. For example, depending on the functions / operations involved, two consecutively shown blocks may actually be executed substantially simultaneously, or the blocks may sometimes be executed in reverse order. Furthermore, the embodiments presented and described in the flowcharts of this application are provided by way of example to provide a more comprehensive understanding of the technology. The disclosed methods are not limited to the operations and logic flows presented herein. Alternative embodiments are contemplated in which the order of various operations is changed and sub-operations described as part of a larger operation are executed independently.

[0065] The embodiments of this application have been described in detail above with reference to the accompanying drawings. However, this application is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of this application. Furthermore, unless otherwise specified, the embodiments and features described in the embodiments of this application can be combined with each other.

Claims

1. A fungal fermentation culture device, characterized in that, include: The box body has an inner cavity for placing a fermentation container. The box body is provided with an operation window, and the operation window is provided with an operation component, through which the operation is performed inside the box body. An external circulation assembly includes an air intake channel and an external circulation channel. The air intake channel is used to introduce gas into the housing, and the external circulation channel connects the air intake channel with the inner cavity of the housing. An internal circulation assembly includes an internal circulation component located within the housing, the internal circulation component being used to drive the flow of gas within the housing; A buffer device is provided, wherein the housing is provided with a transfer buffer window, and the buffer device is connected to the transfer buffer window.

2. The fungal fermentation culture apparatus according to claim 1, characterized in that, The connection points between the air intake channel and the housing, and the connection points between the external circulation channel and the housing, are located on opposite sides of the housing.

3. The fungal fermentation culture apparatus according to claim 1, characterized in that, The enclosure is made of a flexible membrane structure.

4. The fungal fermentation culture apparatus according to claim 1, characterized in that, The fermentation and cultivation device also includes a humidity control component located inside the chamber, which is used to adjust the ambient humidity inside the chamber.

5. The fungal fermentation culture apparatus according to claim 4, characterized in that, The humidity control assembly includes a humidity control device and a dispersion component connected to each other. The steam generated by the humidity control device is evenly distributed in the inner cavity of the box through the dispersion component.

6. The fungal fermentation culture apparatus according to claim 1 or 3, characterized in that, The enclosure also includes a door structure, which is closable within the enclosure.

7. The fungal fermentation culture apparatus according to claim 1, characterized in that, The operating component includes a flexible extension member and a contouring member, the contouring member being located at the end of the extension member.

8. The fungal fermentation culture apparatus according to claim 3, characterized in that, The fermentation culture device also includes a support frame, and the box body is provided with a connecting structure, through which the box body is detachably connected to the support frame.

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