Uniform-heating polyglutamic acid fermentation tank

Abstract

The utility model discloses a polyglutamic acid fermentation tank capable of uniformly heating, and belongs to the technical field of fermentation tanks. The problems that in the prior art, a polyglutamic acid fermentation tank is uneven in heating, low in dissolved oxygen rate and uneven in dissolved oxygen are solved. The device mainly comprises a tank body, a heating jacket is installed on the outer side of the tank body, a heating outer pipe with the top sealed is installed in the middle in the tank body, the bottom of the heating outer pipe downwards extends out of the tank body, a heating inner pipe with the top sealed is installed in the heating outer pipe, and a heating cavity is formed between the heating outer pipe and the heating inner pipe. The bottom of the heating cavity is sealed through a sealing bottom plate, the sealing bottom plate is connected with the bottom of the heating outer pipe and the bottom of the heating inner pipe, a heat conduction oil filling pipe communicated with the heating cavity is arranged on the sealing bottom plate, a backflow opening is formed in the top of the heating inner pipe, and the backflow opening is connected with a heat conduction oil backflow pipe. And the heat-conducting oil return pipe downwards extends out of the bottom of the heating inner pipe.

Description

Technical Field

[0001] This utility model belongs to the field of fermentation tank technology, and more specifically, it relates to a polyglutamic acid fermentation tank with uniform heating. Background Technology

[0002] Polyglutamic acid (Polyglutamic acid) production requires fermenters, and current applications of these fermenters often suffer from uneven heating, low dissolved oxygen levels, and uneven oxygen distribution. Microbial fermentation for Polyglutamic acid production is highly temperature-sensitive. Uneven heating leads to significant temperature differences between different areas of the fermenter. In areas with excessively low temperatures, microbial enzyme activity decreases, resulting in slower metabolic rates and inhibited growth and reproduction. This leads to inconsistent microbial growth throughout the fermentation system, affecting the fermentation process and efficiency. Conversely, excessively high temperatures can cause denaturation and inactivation of proteins, enzymes, and other biomolecules within the microorganisms, disrupting normal cellular functions. Uneven heating also affects product quality and prolongs the fermentation cycle. Polyglutamic acid production typically relies on aerobic microbial fermentation. When dissolved oxygen levels are low, microorganisms cannot obtain sufficient oxygen for aerobic respiration, resulting in insufficient energy supply, inhibited growth and metabolism, slowed reproduction, and even growth cessation. For example, in the fermentation of Bacillus subtilis for Polyglutamic acid production, insufficient dissolved oxygen delays the logarithmic growth phase and reduces biomass accumulation. Uneven dissolved oxygen in the fermenter can cause differences in the growth environment of microorganisms in different areas of the fermenter. Areas with excessive dissolved oxygen may produce too much reactive oxygen species, which can damage the structure and function of microbial cells. Areas with insufficient dissolved oxygen will restrict the growth of microorganisms, resulting in abnormal cell morphology, such as cell deformation and uneven size, which will affect the fermentation process. Summary of the Invention

[0003] The purpose of this invention is to provide a polyglutamic acid fermenter with uniform heating, so as to overcome the problems of uneven heating, low dissolved oxygen rate and uneven dissolved oxygen in the existing polyglutamic acid fermenters.

[0004] This utility model is achieved using the following technical solution: a polyglutamic acid fermenter with uniform heating, comprising a tank body, a heating jacket installed on the outer side of the tank body, a top-sealed outer heating pipe installed in the middle of the tank body, the bottom of the outer heating pipe extending downwards out of the tank body, a top-sealed inner heating pipe installed inside the outer heating pipe, forming a heating cavity between the outer heating pipe and the inner heating pipe, the bottom of the heating cavity being sealed by a sealing base plate, the sealing base plate being connected to the bottom of the outer heating pipe and the bottom of the inner heating pipe respectively, a heat transfer oil injection pipe connected to the heating cavity being provided on the sealing base plate, a return port being opened at the top of the inner heating pipe, the return port being connected to a heat transfer oil return pipe, the heat transfer oil return pipe extending downwards out of the bottom of the inner heating pipe.

[0005] Furthermore, multiple stiffening plates are installed between the outer heating tube and the inner heating tube.

[0006] Furthermore, the inner heating tube is filled with an insulation layer, and the heat transfer oil return pipe passes downward through the insulation layer.

[0007] Furthermore, a motor is fixedly installed on the upper side of the tank, the motor is connected to a hollow shaft reducer, the hollow shaft of the hollow shaft reducer is connected to a stirring shaft, the stirring shaft extends downward into the tank and is rotatably connected to the tank, the stirring shaft is connected upward to the rotating part of a rotary joint, the fixed part of the rotary joint is connected to an air inlet pipe, the bottom of the stirring shaft is connected to a horizontal pipe communicating with the stirring shaft, the two ends of the horizontal pipe are respectively connected to vertical pipes communicating with the horizontal pipe, the vertical pipe is connected at intervals with multiple branch pipes communicating with the vertical pipe, the branch pipes extend into the inner side of the tank, and nozzles and internal stirring blades are installed on the branch pipes.

[0008] Furthermore, the branch pipes on both sides of the vertical pipe are symmetrically arranged, and the inner ends of the two opposite branch pipes are fixedly connected to a bearing seat, which is mounted on the heating outer pipe by a bearing sleeve.

[0009] Furthermore, a support rod is fixedly connected to the lower side of the middle part of the horizontal tube, and a defoaming paddle is installed on the support rod.

[0010] Furthermore, the fixing part of the rotary joint is fixedly installed on the top of the tank by a bracket.

[0011] Furthermore, multiple external stirring blades are installed at vertical intervals along the vertical pipe, and the external stirring blades extend outward from the tank body.

[0012] Furthermore, a fermentation broth temperature sensor is installed on the side wall of the tank, and the upper and lower sides of the heating jacket are respectively connected to the outlet pipe and the inlet pipe.

[0013] Furthermore, discharge pipes are installed on both sides of the heating outer tube at the bottom of the tank; support legs are installed at the bottom of the tank.

[0014] Compared with the prior art, the beneficial effects of this utility model are:

[0015] 1. This utility model provides uniform heating. It changes the current situation where fermentation tanks only use heating jackets for heating. A heating outer tube is installed inside the fermentation tank, and internal heating is achieved through the heating cavity between the heating outer tube and the heating inner tube, thereby realizing internal and external heating of the tank and making the fermentation liquid inside the tank more uniformly heated.

[0016] 2. This invention can effectively improve the dissolved oxygen rate and ensure uniform dissolved oxygen. This invention installs nozzles on the branch pipes, and multiple branch pipes are arranged vertically at intervals. During fermentation, the air inlet pipe delivers oxygen sequentially through the stirring shaft, horizontal pipe and vertical pipe to the branch pipes, and finally sprays it out from the nozzles on the branch pipes. As multiple nozzles spray oxygen at the same time, the stirring shaft drives the vertical pipe and branch pipe to rotate and stir the fermentation liquid, thereby making the oxygen distribution in the fermentation liquid more uniform and effectively improving the dissolved oxygen rate of the fermentation liquid. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the present invention.

[0018] In the diagram: 1. Tank body; 2. Heating jacket; 3. Outer heating pipe; 4. Inner heating pipe; 5. Heating chamber; 6. Sealing bottom plate; 7. Heat transfer oil filling pipe; 8. Return port; 9. Heat transfer oil return pipe; 10. Rib plate; 11. Insulation layer; 12. Motor; 13. Hollow shaft reducer; 14. Stirring shaft; 15. Rotary joint; 16. Air inlet pipe; 17. Horizontal pipe; 18. Vertical pipe; 19. Branch pipe; 20. Nozzle; 21. Inner stirring blade; 22. Bearing seat; 23. Support rod; 24. Defoaming paddle; 25. Bracket; 26. Outer stirring blade; 27. Fermentation broth temperature sensor; 28. Liquid outlet pipe; 29. ​​Liquid inlet pipe; 30. Discharge pipe; 31. Support leg. Detailed Implementation

[0019] To enable those skilled in the art to better understand the technical solutions of this utility model, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort should fall within the scope of protection of this utility model.

[0020] A polyglutamic acid fermenter with uniform heating includes a tank body 1. The top of the tank body 1 may be provided with structures such as a feed inlet, an inoculation port, an exhaust port, and an observation port. The bottom of the tank body 1 is equipped with support legs 31. A fermentation liquid temperature sensor 27 is installed on the inner side wall of the tank body 1. The bottom of the tank body 1 has discharge pipes 30 installed on both sides of the heating outer pipe 3. The fermenter can discharge material simultaneously through the discharge pipes 30 on both sides.

[0021] A stirring shaft 14 is rotatably mounted on the top of the tank 1 via bearings, and a mechanical seal is provided between the stirring shaft 14 and the tank 1. A motor 12 is fixedly mounted on the upper side of the tank 1, and the motor 12 is connected to a hollow shaft reducer 13. The hollow shaft of the hollow shaft reducer 13 is connected to the stirring shaft 14. The motor 12 drives the hollow shaft reducer 13 to rotate, and the hollow shaft reducer 13 drives the stirring shaft 14 to rotate through the hollow shaft. The hollow shaft and the stirring shaft 14 can be connected by means of a key or other transmission method.

[0022] The upper and lower sides of the stirring shaft 14 pass through the upper and lower ends of the hollow shaft, respectively. The stirring shaft 14 has a hollow cavity. The top of the stirring shaft 14 is connected to the rotating part of the rotary joint 15. The fixed part of the rotary joint 15 is connected to the air inlet pipe 16. A valve is installed on the air inlet pipe 16. The fixed part of the rotary joint 15 is fixedly installed on the top of the tank body 1 by the bracket 25. When the stirring shaft 14 rotates, it will drive the rotating part of the rotary joint 15 to rotate. Oxygen in the air inlet pipe 16 is delivered to the stirring shaft 14 through the rotary joint 15.

[0023] The bottom of the stirring shaft 14 is connected to a horizontal pipe 17 that communicates with the stirring shaft 14. The two ends of the horizontal pipe 17 are respectively connected to vertical pipes 18 that communicate with the horizontal pipe 17. The vertical pipe 18 is connected to multiple branch pipes 19 that communicate with the vertical pipe 18 at intervals along the vertical direction. The branch pipes 19 extend into the tank body 1. The branch pipes 19 are equipped with nozzles 20 and inner stirring blades 21.

[0024] Oxygen from the air inlet pipe 16 enters the horizontal pipe 17 via the stirring shaft 14, and is then transported from the horizontal pipe 17 to the vertical pipes 18 on both sides. The vertical pipes 18 then transport the oxygen to the branch pipes 19, and finally spray it out from the nozzles 20 of the branch pipes 19 into the fermentation liquid inside the tank 1. Because the stirring shaft 14 drives the vertical pipes 18 and branch pipes 19 to rotate and stir the fermentation liquid while multiple nozzles 20 are spraying oxygen, the oxygen distribution in the fermentation liquid is more uniform, and the dissolved oxygen rate of the fermentation liquid is effectively improved.

[0025] The vertical pipe 18 is equipped with multiple external stirring blades 26 at vertical intervals, and the external stirring blades 26 extend outward from the tank body 1; an internal stirring blade 21 is installed on the upper and lower sides of the branch pipe 19 respectively. By installing internal stirring blades 21 and external stirring blades 26 on the branch pipe 19, and with the internal stirring blades 21 and external stirring blades 26 arranged opposite to each other, the fermentation liquid in the tank body 1 can be fully stirred.

[0026] The branch pipes 19 on both sides of the vertical pipe 18 are symmetrically arranged, and the inner ends of the two opposite branch pipes 19 are fixedly connected to a bearing seat 22. The bearing seat 22 is mounted on the heating outer pipe 3 by bearings. By connecting the two opposite branch pipes 19 on both sides to the bearing seat 22, this utility model can improve the stability of the horizontal pipe 17 and the two vertical pipes 18 during stirring.

[0027] A support rod 23 is fixedly connected to the lower side of the middle part of the horizontal tube 17, and a defoaming paddle 24 is installed on the support rod 23. By setting the defoaming paddle 24, which can rotate with the stirring shaft 14, this utility model can defoam the foam generated in the tank 1.

[0028] A heating jacket 2 is installed on the outside of the tank body 1. The upper and lower sides of the heating jacket 2 are connected to an outlet pipe 28 and an inlet pipe 29, respectively. Heat transfer oil is added to the heating jacket 2 through the inlet pipe 29, and the heat transfer oil in the heating jacket 2 is refluxed and heated through the outlet pipe 28. The heating jacket 2 can adopt the structural form of heating jacket 2 commonly used in fermenters in the prior art.

[0029] A heating outer tube 3 with a sealed top is installed in the middle of the tank body 1, and the bottom of the heating outer tube 3 extends downward out of the tank body 1. In this invention, the bottom of the heating outer tube 3 passes through the bottom of the tank body 1, and the heating outer tube 3 can be fixed to the bottom of the tank body 1 by welding.

[0030] The heating outer tube 3 is equipped with a heating inner tube 4 with a top-sealed end. The outer diameter of the heating inner tube 4 is smaller than the inner diameter of the heating outer tube 3. The heating inner tube 4 is located in the middle of the heating outer tube 3, and the axes of the heating inner tube 4 and the heating outer tube 3 coincide.

[0031] A heating cavity 5 is formed between the outer heating tube 3 and the inner heating tube 4. The bottom of the heating cavity 5 is sealed by a sealing base plate 6. The sealing base plate 6 is connected to the bottom of the outer heating tube 3 and the bottom of the inner heating tube 4 respectively. The sealing base plate 6 can be connected to the bottom of the outer heating tube 3 and the bottom of the inner heating tube 4 respectively by welding. The sealing base plate 6 seals the heating cavity 5.

[0032] The sealing base plate 6 is provided with a heat transfer oil injection pipe 7 that connects to the heating chamber 5. In this invention, heat transfer oil is injected into the heating chamber 5 through the heat transfer oil injection pipe 7. The heat transfer oil can heat the heating chamber 5, and then heat the inside of the fermenter through the heating outer pipe 3.

[0033] The top of the heating inner tube 4 has a return port 8, which connects to a heat transfer oil return pipe 9, extending downwards from the bottom of the heating inner tube 4. During the process of adding heat transfer oil to the heating chamber 5 through the heat transfer oil filling pipe 7, the oil level in the heating chamber 5 continuously rises until the oil flows from the return port 8 to the heat transfer oil return pipe 9, returning to the heating equipment. This invention heats the heating chamber 5 and the heating jacket 2 through the circulating flow of heat transfer oil.

[0034] Multiple stiffeners 10 are installed between the outer heating tube 3 and the inner heating tube 4. The inner end of the stiffener 10 can be connected to the outer wall of the inner heating tube 4 by welding, and the outer end of the stiffener 10 contacts the inner wall of the inner heating tube 4. By setting the stiffeners 10, the positioning of the inner heating tube 4 can be facilitated during installation.

[0035] The heating inner tube 4 is filled with an insulation layer 11, and the heat transfer oil return pipe 9 passes downward through the insulation layer 11. The insulation layer 11 can be made of polyurethane foam. By filling the heating inner tube 4 with an insulation layer 11, this invention minimizes heat loss from the heating chamber 5. The sealing base plate 6 seals the bottom of the heating inner tube 4, and the heat transfer oil return pipe 9 passes downward through the sealing base plate 6.

Claims

1. A polyglutamic acid fermentation tank capable of heating homogeneously, characterized by, The utility model provides a kind of fermentation tank, including tank body (1), heating jacket (2) is installed on the outside of the tank body (1), heating outer tube (3) is installed in the middle of the tank body (1), the bottom of heating outer tube (3) extends tank body (1) downward, heating inner tube (4) is installed in heating outer tube (3), heating cavity (5) is formed between heating outer tube (3) and heating inner tube (4), the bottom of heating cavity (5) is sealed by sealing bottom plate (6), sealing bottom plate (6) is connected with the bottom of heating outer tube (3) and the bottom of heating inner tube (4) respectively, the sealing bottom plate (6) is provided with heat conducting oil filling pipe (7) that communicates heating cavity (5), the top of heating inner tube (4) is provided with backflow port (8), backflow port (8) is connected with heat conducting oil backflow pipe (9), and the heat conducting oil backflow pipe (9) extends the bottom of heating inner tube (4) downward.

2. The polyglutamic acid fermentation tank with uniform heating according to claim 1, wherein A plurality of rib plates (10) are installed between the heating outer tube (3) and the heating inner tube (4).

3. The polyglutamic acid fermentation tank with uniform heating according to claim 1, characterized by, The heating inner tube (4) is filled with a heat preservation layer (11), and the heat conducting oil backflow pipe (9) passes downward through the heat preservation layer (11).

4. The polyglutamic acid fermentation tank with uniform heating according to claim 1, wherein A motor (12) is fixedly installed on the upper side of the tank body (1), the motor (12) is connected with a hollow shaft speed reducer (13), the hollow shaft of the hollow shaft speed reducer (13) is drivingly connected with a stirring shaft (14), the stirring shaft (14) extends downward into the tank body (1) and is rotatably connected with the tank body (1), the stirring shaft (14) is upwardly connected with a rotating part of a rotary joint (15), a fixed part of the rotary joint (15) is connected with an air inlet pipe (16), the bottom of the stirring shaft (14) is connected with a horizontal pipe (17) in communication with the stirring shaft (14), both ends of the horizontal pipe (17) are respectively connected with vertical pipes (18) in communication with the horizontal pipe (17), the vertical pipes (18) are vertically spaced apart and connected with a plurality of branch pipes (19) in communication with the vertical pipes (18), the branch pipes (19) extend into the tank body (1), and nozzles (20) and inner stirring blades (21) are installed on the branch pipes (19).

5. The polyglutamic acid fermentation tank with uniform heating according to claim 4, characterized by, The branch pipes (19) on the vertical pipes (18) on both sides are symmetrically arranged, and the inner ends of the two opposite branch pipes (19) are fixedly connected with a bearing seat (22), and the bearing seat (22) is sleeved on the heating outer tube (3) through a bearing sleeve.

6. The polyglutamic acid fermentation tank with uniform heating according to claim 4, wherein A supporting rod (23) is fixedly connected to the lower side of the middle part of the horizontal pipe (17), and a defoaming paddle (24) is installed on the supporting rod (23).

7. The polyglutamic acid fermentation tank with uniform heating according to claim 4, characterized by, The fixed part of the rotary joint (15) is fixedly installed on the top of the tank body (1) through a support (25).

8. The polyglutamic acid fermentation tank with uniform heating according to claim 4, characterized by, A plurality of outer stirring blades (26) are vertically spaced apart and installed on the vertical pipes (18), and the outer stirring blades (26) extend outward from the tank body (1).

9. The heating uniform polyglutamic acid fermenter according to claim 1, wherein A fermentation liquid temperature sensor (27) is installed on the side wall in the tank body (1), and an outlet pipe (28) and an inlet pipe (29) are respectively connected to the upper and lower sides of the heating jacket (2).

10. The heating uniform polyglutamic acid fermenter according to claim 1, wherein An outlet pipe (30) is installed on the bottom of the tank body (1) on both sides of the heating outer tube (3); and a supporting leg (31) is installed on the bottom of the tank body (1).

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