Microorganism vacuum preservation tube

Abstract

The utility model discloses a vacuum preservation tube for microorganisms. The vacuum preservation tube comprises a tube body and two cover bodies, namely a cover body A and a cover body B, which are matched for use, the tube body is sequentially provided with a storage area, a fusing area and an opening area from bottom to top, the cover body A is fixedly connected with the opening area and used for sealing the tube body, an axial through hole is axially formed in the cover body A, a filter plug is arranged in the axial through hole, one end of the filter plug is fixed in the axial through hole, the other end of the filter plug extends into the opening area, and the cover body B is detachably arranged on the cover body and used for sealing the axial through hole. Potential pollution risks in freeze-drying, vacuum and fusing processes of strains in the tube body 1 are eliminated, the stability of long-term storage of the strains is ensured, and the freeze-drying tube has the advantages of being simple in structure, low in cost, convenient to use and practical.

Description

Technical Field

[0001] This utility model provides a microbial vacuum preservation tube, specifically relating to a device for preserving microbial strains, belonging to the field of strain preservation technology. Background Technology

[0002] The use of microbial strains in scientific research is becoming increasingly frequent, and their effective preservation is crucial to ensuring their activity and stability. Improper preservation can easily lead to strain contamination, mutation, or death; therefore, reliable methods are needed to keep strains in a dormant state for extended periods. Currently, commonly used preservation methods include liquid paraffin method, glycerol method, magnetic bead method, slant method, liquid nitrogen method, and vacuum freeze-drying method. Among these, vacuum freeze-drying method has significant advantages in terms of preservation time, strain stability, and storage convenience.

[0003] Most existing vacuum culture tubes for bacterial cultures adopt a straight tube structure, divided into an open area, a fused area, and a culture storage area. Typically, a certain volume of liquid bacterial culture is added to the storage area at the bottom of the tube, and the open area is then plugged with cotton or other filter plugs to prevent contamination while ensuring a certain degree of ventilation. The plugged culture tube is then placed in a freeze dryer for freeze-drying, during which moisture is drained through the filter plug in the open area. After the freeze-drying process is complete, the culture tube is removed, and the filter plug on the outside of the tube is cut off. The open area of ​​the culture tube is then connected to a vacuum pump interface, and the vacuum pump is used to evacuate the air from the tube to maintain a vacuum. Finally, a flame is used to melt the fused area while simultaneously sealing the glass tube, ultimately ensuring that the stored bacterial culture is freeze-dried and in a vacuum state within the tube, allowing for low-temperature storage and guaranteeing the long-term stability of the stored bacterial culture.

[0004] In the above-mentioned practical operation process, the existing vacuum culture preservation tubes have at least the following defects:

[0005] (1) When using scissors to cut off excess filter plugs, the filter plugs may become loose, which may lead to potential contamination risks.

[0006] (2) After the freeze-drying of the strain is completed and the storage tube is taken out of the freeze dryer, it will take a certain amount of time before the vacuum operation can be started. During this interval, because there is air circulation in the filter plug, moisture in the air can easily enter the strain storage area, which will lead to the destruction of the strain drying state, the increase of sample moisture content, and affect the long-term storage stability of the strain.

[0007] In summary, there is an urgent need to design a novel freeze-drying vacuum preservation tube for bacterial strains to address the aforementioned shortcomings.

[0008] In the prior art, for example, the invention patent application with publication number CN115369015A discloses a microbial strain preservation device and method. The improvement of the outer cap of the preservation tube in this application is mainly to reduce the amount of air entering during strain extraction. Meanwhile, the utility model patent with authorization publication number CN207417492U discloses a bottle cap for a microbial strain preservation device, which mainly addresses the connection method between the bottle cap and the tube body. Therefore, the prior art does not provide a technical solution to the above or related problems. Utility Model Content

[0009] The purpose of this invention is to utilize the detachable connection between cover B and cover A, and to place one end of the filter plug in the axial through hole and the other end extending into the opening area, so that the bacteria stored in the tube can always be kept in a closed state. This solves the problem that moisture in the air enters the bacterial storage area and destroys the drying state of the bacteria due to air circulation in the filter plug during the interval between freeze-drying and vacuuming. It also solves the problem of filter plug loosening caused by cutting off excess filter plug before vacuuming, thus avoiding potential contamination risks.

[0010] This utility model is achieved through the following technical solution:

[0011] A microbial vacuum preservation tube includes a tube body and two cooperating caps: cap A and cap B. The tube body is provided with a storage area, a fusion zone and an opening zone from bottom to top. Cap A is detachably connected to the opening zone and is used to seal the tube body. An axial through hole is provided in cap A, and a filter plug is provided in the axial through hole. One end of the filter plug is located in the axial through hole, and the other end extends into the opening zone. Cap B is detachably installed on cap A and is used to seal the axial through hole.

[0012] Preferably, the detachable mounting of cover B onto cover A is achieved as follows: the outer wall of cover A has a protrusion, and the inner wall of cover B has protrusions that cooperate with the protrusion; more preferably, the protrusion has a ring-shaped structure, and the plurality of protrusions are evenly distributed in a ring shape. Preferably, the number of protrusions is not less than three.

[0013] Preferably, the outer wall of the cover A is provided with a groove, which is located at the fastening point between the cover A and the cover B and is used to pry open the cover B.

[0014] Preferably, the filter plug is installed in the axial through hole using one of the following methods:

[0015] (1) The axial through hole is cylindrical, the top of the filter plug is aligned with the top of the axial through hole, and the upper end of the side wall of the filter plug is fixedly connected to the upper end of the inner wall of the axial through hole or integrally formed.

[0016] (2) The axial through hole is cylindrical, and an annular flange is provided on the inner side of the top of the axial through hole. The width of the flange ring is 1 / 10 to 1 / 3 of the diameter of the cover A. The top of the filter plug is fixedly connected to the inner wall of the flange or integrally formed.

[0017] (3) Based on (2) above, the upper end of the side wall of the filter plug is further fixedly connected to or integrally formed with the upper end of the inner wall of the axial through hole, that is, the top and upper end of the side wall of the filter plug are fixedly connected to or integrally formed with the inner wall of the flange and the upper end of the inner wall of the axial through hole respectively, so that the filter plug is more firmly fixed in the cover A.

[0018] In this application, the inner wall of the axial through hole refers to the inner surface of the through hole; the annular width refers to the radial distance between the outer circle and the inner circle of the annular surface, that is, the difference in radii between the two circles.

[0019] A further preferred method for fixing the filter plug to the axial through hole is:

[0020] (1) Adhesion: The top of the filter plug is fixedly connected to the inner wall of the flange by adhesive bonding, and / or the upper end of the side wall of the filter plug is fixedly connected to the upper end of the inner wall of the axial through hole by adhesive bonding.

[0021] (2) Pressing: The upper end of the filter plug sidewall is pressed against the upper end of the axial through hole and / or the inner wall of the flange by expansion.

[0022] (3) Integrated molding: The top of the filter plug is integrally molded with the inner wall of the flange, and / or the upper end of the side wall of the filter plug is integrally molded with the inner wall of the axial through hole.

[0023] Among these considerations, considering the simplicity of the production process, bonding is preferred; considering reuse, a compression-type fixed connection is preferred; and if it is a disposable product, one-piece molding is preferred.

[0024] Preferably, the area at the connection between the filter plug and the inner wall of the axial through hole accounts for 1 / 3 to 2 / 3 of the area of ​​the inner wall of the axial through hole.

[0025] Preferably, the cover A is connected to the opening area of ​​the tube by a thread; more preferably, the lower end of the inner wall of the cover A and the upper end of the opening area of ​​the tube are respectively provided with internal threads and external threads.

[0026] Preferably, the filter plug, cover A and / or cover B are made of plastic, rubber or silicone.

[0027] Preferably, the thickness of cover A is 1-2 cm, and the thickness of cover B is 0.5-1 cm. The thickness refers to the vertical height from the bottom to the top of the cover.

[0028] Preferably, the length of the filter plug is 2-3 cm.

[0029] Preferably, the cover body A and cover body B are further provided with gaskets or sealing rings for sealing.

[0030] Compared with the prior art, this utility model has the following advantages and beneficial effects:

[0031] (1) By detachably setting the cover body B on the cover body A, this utility model plays a temporary sealing role on the axial through hole during the interval between the end of freeze drying and the start of vacuuming, which can prevent air from entering the storage area through the filter plug during this interval and thus affecting the drying state of the strain; at the same time, when the vacuuming operation is started, by removing the cover body B, the filter plug can be quickly and conveniently exposed for venting.

[0032] (2) By setting one end of the filter plug in the axial through hole, the present invention can start subsequent operations, including vacuuming, without cutting the filter plug after the strain is freeze-dried, thus avoiding the problem of filter plug loosening caused by cutting the filter plug.

[0033] (3) This utility model forms a snap-fit ​​structure between the cover body B and the cover body A by providing a ring-shaped protrusion on the outer wall of the cover body A and multiple protrusions on the inner wall of the cover body B that cooperate with the protrusion, which is convenient and practical. Preferably, the multiple protrusions are evenly distributed in a ring shape, which makes it easier and less effort to close the cover body B, and the cover body B can be more stably closed on the cover body A, which not only makes the operation more convenient, but also ensures the airtightness between the cover body A and the cover body B. In addition, by providing a groove on the outer wall of the cover body A and setting the groove at the snap-fit ​​point of the cover body A and the cover body B, the cover body B can be opened more easily and conveniently.

[0034] (4) By aligning the filter plug with the top of the axial through hole and fixing or integrally forming the upper end of the filter plug with the upper end of the inner wall of the axial through hole, this utility model can effectively prevent air from entering the tube body through the gap between the filter plug and the axial through hole; wherein, integral forming can further enhance the sealing effect. In addition, by setting the area of ​​the connection between the filter plug and the inner wall of the axial through hole to between 1 / 3 and 2 / 3 of the area of ​​the inner wall of the axial through hole, the filter plug can be effectively prevented from loosening or moving from the axial through hole, without hindering the cover A from covering the tube body, and space can be reserved for setting the thread required for the cover.

[0035] (5) This utility model sets the axial through hole as a cylindrical structure and sets an annular flange on the inner side of the top of the axial through hole. The flange width is set to between 1 / 10 and 1 / 3 of the diameter of the cover A, which makes it easy for the top of the filter plug to be fixedly connected to the inner wall of the flange or integrally formed with the flange. This can prevent the filter plug from loosening or moving from the axial through hole, while retaining the vent and maximizing the air permeability area and efficiency, thus facilitating the effective removal of moisture.

[0036] (6) By detachably connecting the cover A to the opening area of ​​the tube, the cover A can be disinfected and reused, thus avoiding waste of resources; preferably, the cover A and the opening area are provided with internal and external threads to ensure the sealing effect of the tube.

[0037] (7) By providing gaskets or sealing rings for sealing in the cover A and cover B, this utility model can avoid gaps when cover A is covered on the tube body or cover B is covered on cover A, thereby further enhancing the sealing effect.

[0038] In summary, this invention features a simple structure, low cost, and ease of use. It not only solves the problem of moisture in the air entering the strain storage area and damaging the dry state of the strain due to air circulation in the filter plug during the freeze-drying and vacuuming intervals, but also solves the problem of filter plug loosening when removing excess filter plug before vacuuming. This avoids potential contamination risks during the preparation of freeze-dried strains, thus ensuring the stability of long-term strain storage and possessing high practical value. Attached Figure Description

[0039] Figure 1 This is a schematic diagram of the structure of this utility model;

[0040] Figure 2 This is a schematic diagram of the structure of cover A and cover B in this utility model;

[0041] Figure 3 This is a cross-sectional view of the cover body A (with a filter plug inserted in the axial through hole) in this utility model.

[0042] Figure 4 This is a schematic diagram of the inverted cover B in this utility model;

[0043] Wherein, 1—pipe body, 2—cover A, 3—cover B, 4—storage area, 5—fuse area, 6—opening area, 7—axial through hole, 8—filter plug, 9—protrusion, 10—protrusion, 11—groove, 12—flanged edge. Detailed Implementation

[0044] The present invention will be further described in detail below with reference to the embodiments, but the implementation of the present invention is not limited thereto.

[0045] Example 1:

[0046] This embodiment discloses a microbial vacuum preservation tube, including a tube body 1 and two cooperating caps: cap A2 and cap B3; the tube body 1 is provided with a storage area 4, a fusion zone 5 and an opening zone 6 from bottom to top; cap A2 is detachably connected to the opening zone 6 and is used to seal the tube body 1; an axial through hole 7 is provided in the axial through hole 7; a filter plug 8 is provided in the axial through hole 7; one end of the filter plug 8 is fixed in the axial through hole 7 and the other end extends into the opening zone 6; cap B3 is detachably mounted on cap A2 and is used to seal the axial through hole 7.

[0047] Furthermore, in this embodiment, to prevent the filter plug 8 from coming loose from the axial through hole 7 during use, the thickness of the cover A2 is set to 1~2cm, the thickness of the cover B is set to 0.5~1cm, and the length of the filter plug 8 is set to 2~3cm. This ensures that the filter plug 8 has sufficient contact area with the upper end of the inner wall of the axial through hole 7 (that is, the upper end of the inner wall of the cover A2), and at the same time, it ensures that the filter plug 8 inserted into the opening area 6 has sufficient length, thereby ensuring the filtration and drainage effect of the filter plug 8.

[0048] Furthermore, considering the sealing issue during the use of pipe body 1, the following methods are adopted to enhance the sealing effect of pipe body 1:

[0049] 1. The filter plug 8 is fixed in the axial through hole 7 by means of bonding, expansion compression, integral molding, etc., to prevent the filter plug 8 from loosening or falling off the axial through hole 7, and to effectively avoid gaps between the filter plug 8 and the axial through hole 7:

[0050] (1) Adhesion: The upper end of the filter plug 8 is fixed to the upper end of the inner wall of the axial through hole 7 by adhesive.

[0051] (2) Expansion and compression: The upper end of the filter plug 8 is fixed to the upper end of the inner wall of the axial through hole 7 by expansion and compression, which can avoid the use of adhesive materials and the potential risk of contamination of bacteria.

[0052] (3) Integrated molding: The upper end of the filter plug 8 is integrally molded with the upper end of the inner wall of the axial through hole 7 (that is, the upper end of the inner wall of the cover A2).

[0053] 2. Use filter plugs 8, caps A2, and caps B3 made of plastic, natural rubber, or silicone materials to make the overall structure of the three components more stable by utilizing their compressibility and plasticity.

[0054] 3. Optionally, a gasket or sealing ring may be provided inside the cover body A2 and cover body B2 to prevent gaps from forming between cover body A2 and tube body 1, or between cover body B3 and cover body A2, after the cover body is fastened. Providing a gasket or sealing ring inside the cover body is standard practice and will not be elaborated further here.

[0055] In this embodiment, the filter plug 8 is specifically bonded to the upper end of the inner wall of the axial through hole 7, and the area of ​​the bonding point accounts for 1 / 3 to 2 / 3 of the area of ​​the inner wall of the axial through hole 7, preferably 1 / 3, so as to effectively ensure the firmness of the bonding.

[0056] It should be noted that, to ensure a good seal after the cap A2 is closed to the opening area 6 of the tube 1, the following method can be used:

[0057] (1) In the absence of other sealing devices, the diameter of the filter plug 8 used should be equal to or slightly larger than the inner diameter of the tube body 1 to enhance the airtightness of the tube body 1.

[0058] (2) If a gasket or sealing ring is provided inside the cover A2 for sealing, the diameter of the filter plug 8 can be appropriately reduced.

[0059] In this embodiment, considering that the portion above the opening area 6, including the opening area 6, cover A2, cover B3 and filter plug 8, will be discarded when used once, and for cost-saving reasons, no sealing device such as a gasket or sealing ring is provided in the cover A2 and cover B2. Therefore, a filter plug 8 with a diameter equal to or slightly larger than the inner diameter of the tube 1 is used.

[0060] In this embodiment, the cover A2 and the opening area 6 are detachably connected, meaning that the cover A2 can be removed from the opening area 6 and reused, which improves resource utilization and avoids resource waste. Of course, before reuse, the cover A2 needs to be sterilized to prevent contamination of the subsequently preserved microorganisms.

[0061] Example 2:

[0062] To facilitate the removal of cover B3 from cover A2, this embodiment further optimizes cover A2: such as... Figure 1 , Figure 2 As shown, a groove 11 for prying open the cover B3 is provided on the outer wall of the cover A2. The groove 11 is located at the point where the cover A2 and the cover B3 are fastened together. In use, place your fingertip in the groove 11 and apply upward force to easily remove the cover B3.

[0063] Example 3:

[0064] To facilitate the fitting of the cover A2 onto the tube 1, this embodiment further optimizes the cover A2: as follows... Figure 2 , Figure 3 As shown, in this embodiment, the axial through hole 7 is set as a cylindrical structure, and an annular flange 12 is provided on the inner side of the top of the axial through hole 7. The width of the flange 12 is 1 / 10 to 1 / 3 of the diameter of the cover A2. The top of the filter plug 8 is fixedly connected to the inner wall of the flange 12 or integrally formed to realize the connection between the filter plug 8 and the cover A2.

[0065] Furthermore, with the upper end of the filter plug 8 already connected to the upper end of the inner wall of the axial through hole 7, this optimized design can further increase the stability of the connection between the cover A2 and the filter plug 8. In this embodiment, the filter plug 8 is not directly connected to the inner wall of the axial through hole 7; the filter plug 8 is simply fixed to the inner wall of the flange 12. That is, there can be a certain gap between the side wall of the filter plug 8 and the axial through hole 7, so that the cover A2 can be more conveniently and effortlessly fitted onto the tube 1.

[0066] Example 4:

[0067] To facilitate the application and removal of the cover A2 onto or from the tube 1, this embodiment further optimizes the cover A2 and the opening area 6 of the tube 1: such as Figure 1 and Figure 3 As shown, in this embodiment, the cover A2 and the opening area 6 are provided with internal and external threads. The lower end of the cover A2 is provided with an internal thread, and the outer wall of the opening area 6 is provided with an external thread. The internal and external threads work together to fix the cover A2 onto the opening area 6, thereby closing the tube 1 and achieving a detachable connection between the cover A2 and the opening area 6. In this embodiment, the internal thread is provided at the lower end of the inner wall of the axial through hole 7 and occupies between 1 / 3 and 2 / 3 of the inner wall area of ​​the axial through hole 7. Of course, an external thread can also be provided on the outer wall of the cover A2 and an internal thread can be provided on the inner wall of the tube 1 corresponding to the opening area 6 to achieve a threaded connection.

[0068] Example 5:

[0069] To improve ease of use, this embodiment further optimizes lid A2 and lid B3: such as Figure 2 , Figure 3 and Figure 4 As shown, cover A2 and cover B3 are respectively provided with a ring of protrusions 9 and multiple protrusions 10 for use in conjunction to achieve mutual locking of cover A2 and cover B3 (or threads are provided on the mating surfaces of cover A2 and cover B3, allowing them to be tightened or partially tightened, replacing the aforementioned ring of protrusions 9 and multiple protrusions 10). The ring of protrusions 9 is provided on the outer wall of cover A2, forming a ring structure, and is parallel to the upper edge of cover A2; the multiple protrusions 10 are provided on the inner wall of cover B3, evenly distributed in a ring. To save effort during locking, the protrusions 10 should not be too large, so that when cover B3 is locked onto cover A2, only a slight force is needed for the multiple protrusions 10 to engage below the protrusions 9. Of course, in addition to the above-mentioned method of using the protrusion 9 of the cover A2 and the protrusion 10 of the cover B3 to form a fastening, any structure that can make the cover B3 cover and fix it on the cover A2 is applicable to this utility model.

[0070] The usage method of this embodiment is as follows:

[0071] Method 1:

[0072] Step 1: First, add the liquid bacterial culture to the storage area 4 inside the tube 1, then insert the filter plug 8 on the cap A2 into the opening area 6, and fix the cap A2 on the opening area 6 to seal the tube 1.

[0073] Step 2: Place the tube 1 containing the bacterial culture into a freeze dryer for freeze drying of the bacterial culture. During the freeze drying process, the water is discharged through the filter plug 8 in the opening area 6.

[0074] Step 3: After the freeze-drying process is completed, remove tube 1, and then detachably fix cover B3 onto cover A2 and seal the axial through hole 7.

[0075] Step 4: When vacuuming the tube containing the bacteria, remove the cover B3 to expose the filter plug 8 on the top of the cover A2, and connect the top of the cover A2 to the vacuum pump interface to perform the vacuuming operation until the set requirements are met. Then, use a water-stop clamp to seal the vacuum connection hose.

[0076] Step 5: Weld the sealing tube 1 through the fusing zone 5 as required.

[0077] Method 2:

[0078] Step 1: First, add the liquid bacterial culture to the storage area 4 inside tube 1. The cap B3 is detachably fixed to the cap A2. Then, insert the filter plug 8 on the cap A2 into the opening area 6. The cap A2 is partially tightened and fixed to the opening area 6 (not completely tightened to allow moisture to escape through the gap).

[0079] Step 2: Place the tube 1 containing the bacterial culture into a freeze dryer for freeze drying of the bacterial culture. During the freeze drying process, the water passes through the filter plug 8 of the opening area 6 and is discharged through the gap between the cover A2 and the opening area 6.

[0080] Step 3: After the freeze-drying process is completed, remove tube 1 and then tighten the cap A2 completely onto the opening area 6 to seal tube 1.

[0081] Step 4: When vacuuming the tube containing the bacteria, remove the cover B3 to expose the filter plug 8 on the top of the cover A2, and connect the top of the cover A2 to the vacuum pump interface to perform the vacuuming operation until the set requirements are met. Then, use a water-stop clamp to seal the vacuum connection hose.

[0082] Step 5: Weld the sealing tube 1 through the fusing zone 5 as required.

[0083] By using the above method, a vacuum freeze-dried strain that can be sealed in tube 1 and preserved for a long time can be obtained, thus ensuring the stability of the strain during long-term storage.

[0084] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any way. Any simple modifications or equivalent changes made to the above embodiments based on the technical essence of the present utility model shall fall within the protection scope of the present utility model.

Claims

1. A vacuum preservation tube for microorganisms, characterized in that, It includes a tube body (1) and two cooperating covers: cover A (2) and cover B (3); the tube body (1) is provided with a storage area (4), a fusing area (5) and an opening area (6) from bottom to top. Cover A (2) is detachably connected to the opening area (6) and used to close the tube body (1). An axial through hole (7) is provided in the cover A (2). A filter plug (8) is provided in the axial through hole (7). One end of the filter plug (8) is located in the axial through hole (7) and the other end extends into the opening area (6). Cover B (3) is detachably installed on cover A (2) and used to close the axial through hole (7).

2. The microbial vacuum preservation tube according to claim 1, characterized in that, The outer wall of the cover A (2) is provided with a protrusion (9), and the inner wall of the cover B (3) is provided with a protrusion (10) that works in conjunction with the protrusion (9) and is used to fasten with the cover A (2).

3. The microbial vacuum preservation tube according to claim 2, characterized in that, The protrusion (9) has a ring structure, and the protrusions (10) are provided in multiple ways and are evenly distributed in a ring.

4. A microbial vacuum preservation tube according to claim 1, characterized in that, The outer wall of the cover A (2) is provided with a groove (11), which is located at the fastening point between the cover A (2) and the cover B (3) and is used to pry open the cover B (3).

5. A microbial vacuum preservation tube according to claim 1, characterized in that, The top of the filter plug (8) is aligned with the top of the axial through hole (7), and the upper end of the side wall of the filter plug (8) is fixedly connected to or integrally formed with the upper end of the inner wall of the axial through hole (7).

6. A microbial vacuum preservation tube according to claim 5, characterized in that, The area at the connection between the filter plug (8) and the inner wall of the axial through hole (7) is between 1 / 3 and 2 / 3 of the area of ​​the inner wall of the axial through hole (7).

7. A microbial vacuum preservation tube according to claim 1, characterized in that, The axial through hole (7) has a cylindrical structure and a ring-shaped flange (12) is provided on the inner side of the top of the axial through hole (7). The width of the flange (12) is 1 / 10 to 1 / 3 of the diameter of the cover body A (2). The top of the filter plug (8) is fixedly connected to the inner wall of the flange (12) or integrally formed.

8. A microbial vacuum preservation tube according to claim 7, characterized in that, The upper end of the side wall of the filter plug (8) is fixedly connected to the upper end of the inner wall of the axial through hole (7) or integrally formed.

9. A microbial vacuum preservation tube according to claim 8, characterized in that, The area at the connection between the filter plug (8) and the inner wall of the axial through hole (7) is between 1 / 3 and 2 / 3 of the area of ​​the inner wall of the axial through hole (7).

10. A microbial vacuum preservation tube according to any one of claims 1 to 9, characterized in that, The cover A (2) and the opening area (6) are connected by threads.

11. A microbial vacuum preservation tube according to any one of claims 1 to 9, characterized in that, The filter plug (8), cover A (2) and / or cover B (3) are made of plastic, rubber or silicone.

12. A microbial vacuum preservation tube according to any one of claims 1 to 9, characterized in that, The thickness of the cover A (2) is 1~2cm, the thickness of the cover B (3) is 0.5~1cm, and the length of the filter plug (8) is 2~3cm.

13. A microbial vacuum preservation tube according to any one of claims 1 to 9, characterized in that, The cover A (2) and cover B (3) are also provided with gaskets or sealing rings for sealing.

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