Reaction cup for in-vitro diagnostic equipment

Abstract

The utility model discloses a reaction cup for in vitro diagnostic equipment, which relates to the field of chemiluminescence immunoassay equipment, and comprises an outer tube used for collection and an inner tube inserted in the outer tube and used for separation, separation magnetic beads are arranged in the inner tube, a filter component is arranged at the bottom of the inner tube, and the filter component is arranged in the inner tube. The separation magnetic beads in the inner pipe cannot penetrate through the filtering assembly; the outer pipe and the inner pipe each comprise a main body and two side lugs fixedly arranged on the two sides of the top of the main body, the two side lugs and the main bodies are arranged in a T shape, through the nested design of the inner pipe and the outer pipe, space can be effectively utilized, and meanwhile efficient separation of the separation magnetic beads and liquid is achieved. The filtering assembly at the bottom of the inner pipe ensures that the separation magnetic beads are blocked in the inner pipe, and liquid smoothly flows into the outer pipe, so that complexity and equipment dependence caused by traditional centrifugal operation are avoided. In addition, the design can be seamlessly matched with the existing chemiluminescence immunoassay instrument, and the detection efficiency and the automation degree are remarkably improved.

Description

Technical Field

[0001] This utility model relates to the technical field of chemiluminescence immunoassay analyzers, and more specifically, to a reaction cup for use in in vitro diagnostic equipment. Background Technology

[0002] Chemiluminescence immunoassay analyzers have been widely used in medical testing and related scientific analysis research. Based on the principle of antigen-antibody specific recognition, chemiluminescence immunoassay involves labeling antibodies (antigens) onto solid-phase media such as magnetic microparticles and tracer substances, respectively, to capture and trace the antigen (antibody) to be detected. The analyte is quantified by reading the chemiluminescence value excited by the tracer substance. Fully automated chemiluminescence immunoassay analyzers are essential instruments for performing chemiluminescence immunoassays. They utilize automated mechanical equipment to perform tasks such as loading and unloading reaction cups, quantitative addition of samples and reagents, mixing, incubation, magnetic attraction, washing, reading, and conversion of signal values ​​during the detection process.

[0003] However, in practical applications, an immunoassay system often includes hundreds of items, and not all analytes can be directly captured and traced through antigen-antibody reactions. Especially for analytes with low protein abundance or complex protein compositions in body fluids, functional extraction magnetic beads are needed for extraction, impurity removal, and elution to ensure the analyte is present in the elution supernatant for routine chemiluminescent immunoassay. However, the process of extracting the supernatant and transferring it to a new sample cup conflicts significantly with the existing automated operating procedures of the immunoassay analyzer, severely impacting its throughput and reducing detection efficiency.

[0004] While some existing reaction cup designs attempt to address this issue, most have limitations. For example, some reaction cups, although improving the stability of sample collection and transfer within the immunoassay analyzer, do not address the function of separating magnetic beads from the supernatant. Other solutions that do achieve separation, such as pre-loaded centrifuge columns, often require centrifugation, which is complex and incompatible with existing chemiluminescence immunoassay analyzers, thus failing to achieve fully automated high-throughput detection.

[0005] In summary, how to provide a reaction cup that can effectively separate magnetic beads from supernatant, and is compatible with existing chemiluminescence immunoassay analyzers to achieve fully automated high-throughput detection is a problem that urgently needs to be solved by those skilled in the art. Utility Model Content

[0006] In view of this, the purpose of this utility model is to provide a reaction cup for in vitro diagnostic equipment, which effectively separates magnetic beads from supernatant and can be well adapted to existing chemiluminescence immunoassay analyzers to achieve fully automated high-throughput detection.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A reaction cup for an in vitro diagnostic device includes an outer tube for collection and an inner tube inserted inside the outer tube for separation. The inner tube contains a separation magnetic bead, and a filter assembly is provided at the bottom of the inner tube to prevent the separation magnetic bead in the inner tube from passing through the filter assembly.

[0009] Both the outer tube and the inner tube include a main body and two side ears fixedly disposed on both sides of the top of the main body. The two side ears are arranged in a T-shape with the main body.

[0010] Preferably, the filtration assembly includes a filter cloth disposed at the bottom of the inner tube, and a fixing assembly is provided inside the inner tube to fix the filter cloth to the inner tube.

[0011] Preferably, the fixing component includes a clamping washer disposed inside the inner tube, the clamping washer being disposed along the inner wall of the bottom of the inner tube, so as to fix the filter cloth to the inner tube.

[0012] Preferably, the fixing component includes a rubber ring disposed inside the inner tube to fix the filter cloth to the inner tube.

[0013] Preferably, the filter assembly includes a filter cloth disposed at the bottom of the inner tube, the filter cloth being adhered to the inner wall of the inner tube.

[0014] Preferably, the filter assembly includes a filter element disposed at the bottom of the inner tube, and the filter element is fixedly connected to the inner tube.

[0015] Preferably, a sealing ring is wound around the bottom of the inner tube, and the side of the sealing ring away from the inner tube abuts against the inner wall of the outer tube.

[0016] Preferably, a first baffle is provided on the outer side of the inner tube, on the side opposite to the bottom of the outer tube to the sealing spacer, and the first baffle is in contact with the sealing spacer.

[0017] Preferably, a second baffle is provided on the outside of the inner tube on the other side of the sealing spacer, and the first baffle and the second baffle clamp the sealing spacer between them.

[0018] Preferably, an installation groove is provided on the outer wall of the inner tube, and the sealing spacer is disposed in the installation groove.

[0019] The reaction cup for in vitro diagnostic equipment provided by this invention effectively utilizes space through a nested design of inner and outer tubes, while simultaneously achieving efficient separation of magnetic beads and liquid. A filter assembly at the bottom of the inner tube ensures that the magnetic beads are trapped within the inner tube, while the liquid flows smoothly into the outer tube, avoiding the complexity and equipment dependence of traditional centrifugation operations. Furthermore, this design seamlessly adapts to existing chemiluminescence immunoassay analyzers, significantly improving detection efficiency and automation, reducing manual intervention, thereby minimizing operational errors and enhancing the reliability of test results.

[0020] The further solutions provided in this application can also achieve at least one of the following beneficial technical effects:

[0021] The sealing ring installed on the outside of the bottom of the inner tube can seal the gap between the inner and outer tubes in the nested state, effectively preventing liquid residue, ensuring the thoroughness of the washing process, and thus improving the accuracy of the test results.

[0022] The negative pressure environment created by the sealing ring accelerates the process of liquid in the inner tube passing through the filter cloth into the outer tube, improving the liquid transfer efficiency. At the same time, it is compatible with the working requirements of a fully automated chemiluminescence immunoassay analyzer, significantly improving the detection throughput and automation level. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0024] Figure 1 This is a side cross-sectional view of the reaction vessel in this embodiment;

[0025] Figure 2 This is a schematic diagram of the structure in this embodiment where only the first baffle is provided;

[0026] Figure 3 This is a schematic diagram of the structure of the first baffle and the second baffle in this embodiment;

[0027] Figure 4 This is a schematic diagram of the structure with the mounting slot in this embodiment.

[0028] Figures 1-4 In the accompanying drawings, the reference numerals include:

[0029] 1. Outer tube; 2. Inner tube; 3. Filter cloth; 4. Clamping gasket; 5. Sealing spacer; 6. First baffle; 7. Second baffle; 8. Mounting groove. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Unless otherwise defined, the technical or scientific terms used in this application shall have the ordinary meaning understood by one of ordinary skill in the art to which this utility model pertains. The terms "first," "second," and similar words used in this utility model do not indicate any order, quantity, or importance. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; when the absolute position of the described object changes, the relative positional relationship may also change accordingly. An embodiment of this application discloses a reaction cup for an in vitro diagnostic device.

[0032] The core of this invention is to provide a reaction cup for in vitro diagnostic equipment.

[0033] Please refer to Figure 1 .

[0034] The reaction cup for in vitro diagnostic equipment provided by this utility model includes an outer tube 1 for collection and an inner tube 2 inserted into the outer tube 1 for separation. A separation magnetic bead is provided in the inner tube 2, and a filter assembly is provided at the bottom of the inner tube 2 so that the separation magnetic bead in the inner tube 2 cannot pass through the filter assembly. Both the outer tube 1 and the inner tube 2 include a main body and two side ears fixedly disposed on both sides of the top of the main body. The two side ears are T-shaped with the main body.

[0035] Specifically, the diameter of the inner tube 2 is smaller than that of the outer tube 1, so the inner tube 2 can be completely nested within the outer tube 1. Both the outer tube 1 and the inner tube 2 include a main body and two side ears fixedly installed on both sides of the top of the main body. The two side ears are T-shaped with the main body, which facilitates the gripper to grasp the side ears of the inner tube 2 and the outer tube 1 and separate them. A filter assembly is installed at the bottom of the inner tube 2. The filter assembly prevents the separation magnetic beads in the inner tube 2 from penetrating the filter assembly, thus keeping them in the inner tube 2. During the separation process by the gripper, the separated tube 2 can be directly discarded into the waste chamber, while the separated liquid (analyte) can pass through the filter assembly into the outer tube 1 and be used as a routine sample for immunoassay.

[0036] It should be noted that the outer tube 1 can be a standard T-type reaction cup used in immunoassay analyzers, while the side ear of the inner tube 2 is mounted on the side ear of the outer tube 1, so that the inner tube 2 and the outer tube 1 can be separated simply by adjusting the height of the gripper of the immunoassay analyzer.

[0037] The aforementioned reaction cup for in vitro diagnostic equipment effectively separates magnetic beads from the supernatant and is well-compatible with existing chemiluminescence immunoassay analyzers, enabling fully automated high-throughput detection. The nested design of the inner tube 2 and outer tube 1 effectively utilizes space while achieving efficient separation of the magnetic beads and liquid. The filter assembly at the bottom of the inner tube 2 ensures that the magnetic beads are trapped within it, while the liquid flows smoothly into the outer tube 1, avoiding the complexity and equipment dependence of traditional centrifugation operations. Furthermore, this design seamlessly integrates with existing chemiluminescence immunoassay analyzers, significantly improving detection efficiency and automation, reducing manual intervention, thereby minimizing operational errors and enhancing the reliability of test results.

[0038] The reaction cup for in vitro diagnostic equipment provided by this utility model will be described in more detail below with reference to the accompanying drawings and specific embodiments.

[0039] In one specific implementation, reference is made to... Figure 1 The filter assembly includes a filter cloth 3 disposed at the bottom of the inner tube 2, and a fixing assembly is provided inside the inner tube 2 to fix the filter cloth 3 to the inner tube 2.

[0040] Specifically, the filter cloth 3 can be made of a high-strength, corrosion-resistant fiber material. This material not only effectively blocks the passage of the separating magnetic beads, but its microporous structure also allows the test liquid to pass through smoothly, ensuring filtration efficiency. The fixing assembly, through a suitable layout and fastening method, ensures the stable installation of the filter cloth 3 at the bottom of the inner tube 2. Even under conditions such as high-speed vibration or strong magnetic separation, it can prevent the filter cloth 3 from shifting or falling off, thereby ensuring the stability and reliability of the filtration effect.

[0041] Furthermore, the fixing component includes a clamping washer 4 disposed inside the inner tube 2, the clamping washer 4 being disposed along the inner wall of the bottom of the inner tube 2, so as to fix the filter cloth 3 to the inner tube 2.

[0042] Specifically, the clamping gasket 4 can be made of highly elastic silicone material. Its shape and size are set according to the size of the inner tube 2 and the filter cloth 3, so that it can fit tightly against the inner wall of the inner tube 2 and the surface of the filter cloth 3. When installing the filter cloth 3, the clamping gasket 4 uses its elastic clamping force to firmly fix the filter cloth 3 to the bottom of the inner tube 2, which not only ensures the filtration effect, but also avoids wear and loosening of the filter cloth 3 during long-term use. At the same time, it also prevents the separating magnetic beads from penetrating the filter cloth 3 and remaining in the inner tube 2.

[0043] Optionally, the fixing component includes a rubber ring (not shown in the figure) disposed inside the inner tube 2 to fix the filter cloth 3 to the inner tube 2.

[0044] Specifically, the clamping washer 4 can be replaced with a rubber ring. The rubber ring has good elasticity and sealing properties, which can tightly wrap the edge of the filter cloth 3 and firmly fix the filter cloth 3 to the bottom of the inner tube 2 through its elastic deformation. It can produce the same beneficial effect as the clamping washer 4.

[0045] In some other embodiments, the filter assembly may also adopt the following scheme: the filter assembly may include a filter cloth 3 disposed at the bottom of the inner tube 2, and the filter cloth 3 is adhered to the inner wall of the inner tube 2.

[0046] Specifically, in addition to the above-mentioned method of fixing the filter cloth 3 by clamping gasket 4 or rubber ring, the filter cloth 3 can also be fixed by adhesive. The adhesive can be a high-strength, corrosion-resistant adhesive that can maintain its bonding effect for a long time. This ensures a strong bond between the filter cloth 3 and the inner tube 2, maintaining a stable filtration effect.

[0047] In some other embodiments, the filter assembly may also adopt the following scheme: the filter assembly includes a filter element (not shown in the figure) disposed at the bottom of the inner tube 2, and the filter element is fixedly connected to the inner tube 2.

[0048] Specifically, in addition to the above-mentioned scheme of using filter cloth 3 as a filter component, filter cloth 3 can also be replaced with a filter element that has a certain shape and hardness, so that the filter element can be directly fixed to the bottom of the inner cavity of the inner tube 2.

[0049] Based on any of the above embodiments, refer to Figures 2 to 4 A sealing ring 5 is provided around the bottom of the inner tube 2, and the side of the sealing ring 5 away from the inner tube 2 abuts against the inner wall of the outer tube 1.

[0050] Specifically, the sealing spacer 5 is disposed between the inner tube 2 and the outer tube 1, which can effectively seal the gap between the inner tube 2 and the outer tube 1 after the inner tube 2 is embedded in the outer tube 1, thereby ensuring that there is no liquid residue between the inner tube 2 and the outer tube 1. The sealing spacer 5 can be a rubber sealing ring.

[0051] It should be noted that the sealing spacer 5 can be fixed to the outside of the bottom of the inner tube 2, and can be fixed by means of bonding or other methods.

[0052] Based on any of the above embodiments, refer to Figure 2 A first baffle 6 is provided on the outside of the inner tube 2 on the side opposite to the bottom of the outer tube 1, and the first baffle 6 is in contact with the sealing ring 5.

[0053] Specifically, a first baffle 6 is provided on the top of the sealing spacer 5, which can effectively facilitate the assembly between the inner tube 2 and the outer tube 1. During the process of removing the inner tube 2 after the reaction is completed, the sealing spacer 5 can slide off the outer wall of the inner tube 2 and fall into the outer tube 1. The rolling friction between the sealing spacer 5 and the inner wall of the outer tube 1 replaces the sliding friction, making it easier to remove the inner tube 2.

[0054] Based on any of the above embodiments, refer to Figure 3 A second baffle 7 is provided on the outside of the inner tube 2 on the other side of the sealing spacer 5, and the first baffle 6 and the second baffle 7 clamp the sealing spacer 5 between them.

[0055] Specifically, the first baffle 6 and the second baffle 7 clamp the sealing ring 5 between them, so that a negative pressure is maintained between the inner tube 2 and the outer tube 1 during the removal of the inner tube 2, thereby accelerating the filtration of liquid in the inner tube 2. After the liquid in the inner tube 2 has completely flowed into the outer tube 1, air can pass through the filter cloth to achieve a pressure balance between the inner and outer tubes 1, preventing the inner tube 2 from being difficult to pull out.

[0056] Based on any of the above embodiments, refer to Figure 4 An installation groove 8 is provided on the outer wall of the inner tube 2, and a sealing spacer 5 is placed in the installation groove 8.

[0057] Specifically, the installation slot 8 is designed to work in conjunction with the first baffle 6 and the second baffle 7, achieving the desired effect. This maintains a negative pressure between the inner tube 2 and the outer tube 1 during the removal of the inner tube 2, thus accelerating the filtration of liquid from the inner tube 2. After the liquid in the inner tube 2 has completely flowed into the outer tube 1, air can pass through the filter cloth to achieve pressure balance between the inner and outer tubes 1, preventing the inner tube 2 from being difficult to remove.

[0058] In summary, this application's embodiment of a reaction cup for in vitro diagnostic equipment achieves efficient separation of magnetic beads and supernatant through a nested design of inner tube 2 and outer tube 1. The filter assembly at the bottom of the inner tube 2 uses high-strength, corrosion-resistant fiber filter cloth, and is secured with clamping gaskets or rubber rings to ensure stable installation and filtration efficiency. Simultaneously, the sealing spacer effectively seals the gap between the inner and outer tubes, preventing liquid residue. Furthermore, the design of the first baffle, second baffle, and mounting groove not only facilitates the assembly of the inner and outer tubes but also maintains a negative pressure state in the gap when removing the inner tube, accelerating liquid filtration and ensuring easy removal of the inner tube. This reaction cup not only simplifies the operation process and improves sample processing efficiency and accuracy but also seamlessly adapts to existing chemiluminescence immunoassay analyzers, achieving fully automated high-throughput detection, significantly reducing operational errors, and improving the reliability of detection results.

[0059] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0060] The above provides a detailed description of a reaction cup for in vitro diagnostic equipment provided by this utility model. Specific examples have been used to illustrate the principle and implementation of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the method and core idea of ​​this utility model. It should be noted that those skilled in the art can make several improvements and modifications to this utility model without departing from the principle of this utility model, and these improvements and modifications also fall within the protection scope of this utility model.

Claims

1. A reaction cup for use in an in vitro diagnostic device, characterized in that, It includes an outer tube (1) for collection and an inner tube (2) inserted into the outer tube (1) for separation. The inner tube (2) is provided with a separation magnetic bead, and a filter assembly is provided at the bottom of the inner tube (2) so that the separation magnetic bead in the inner tube (2) cannot pass through the filter assembly. Both the outer tube (1) and the inner tube (2) include a main body and two side ears fixedly disposed on both sides of the top of the main body. The two side ears are arranged in a T-shape with the main body.

2. The reaction cup for an in vitro diagnostic device according to claim 1, characterized in that, The filter assembly includes a filter cloth (3) disposed at the bottom of the inner tube (2), and a fixing assembly is provided inside the inner tube (2) to fix the filter cloth (3) on the inner tube (2).

3. A reaction cup for an in vitro diagnostic device according to claim 2, characterized in that, The fixing component includes a clamping washer (4) disposed inside the inner tube (2), the clamping washer (4) being disposed along the inner wall of the bottom of the inner tube (2) so that the filter cloth (3) is fixed to the inner tube (2).

4. A reaction cup for an in vitro diagnostic device according to claim 2, characterized in that, The fixing component includes a rubber ring disposed inside the inner tube (2) to fix the filter cloth (3) to the inner tube (2).

5. A reaction cup for an in vitro diagnostic device according to claim 1, characterized in that, The filter assembly includes a filter cloth (3) disposed at the bottom of the inner tube (2), and the filter cloth (3) is adhered to the inner wall of the inner tube (2).

6. A reaction cup for an in vitro diagnostic device according to claim 1, characterized in that, The filter assembly includes a filter element disposed at the bottom of the inner tube (2), and the filter element is fixedly connected to the inner tube (2).

7. A reaction cup for an in vitro diagnostic device according to any one of claims 1-6, characterized in that, A sealing ring (5) is wrapped around the bottom of the inner tube (2), and the side of the sealing ring (5) away from the inner tube (2) abuts against the inner wall of the outer tube (1).

8. A reaction cup for an in vitro diagnostic device according to claim 7, characterized in that, A first baffle (6) is provided on the side of the inner tube (2) away from the bottom of the outer tube (1) and located outside the sealing ring (5). The first baffle (6) is in contact with the sealing ring (5).

9. A reaction cup for an in vitro diagnostic device according to claim 8, characterized in that, A second baffle (7) is provided on the outside of the inner tube (2) on the other side of the sealing ring (5), and the first baffle (6) and the second baffle (7) clamp the sealing ring (5) between them.

10. A reaction cup for an in vitro diagnostic device according to claim 7, characterized in that, An installation groove (8) is provided on the outer wall of the inner tube (2), and the sealing ring (5) is disposed in the installation groove (8).

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