Device for extracting exosome from stem cells of dogs and cats

Abstract

The utility model provides a dog and cat stem cell exosome extraction device, and belongs to the technical field of stem cells. The dog and cat stem cell exosome extraction device comprises a transparent mixing tank and a filtering barrel, a rotating part is arranged at an opening of the filtering barrel, the mixing tank rotates on the rotating part, a communicating pipe is arranged in the mixing tank in a penetrating and lifting mode, an overflow opening is formed in the middle of the communicating pipe in a penetrating mode, and the overflow opening is communicated with the filtering barrel. And the mixing tank is conditionally communicated with the filtering barrel through the communicating pipe. The exosome extraction device has the following effects that the overflow port sinks to a proper depth of a solution through the ascending and descending of the communicating pipe, and a supernatant rich in exosomes passes through the communicating pipe from the overflow port and enters the filtering barrel below for filtering, so that impurities such as cell debris and dead cells participating in filtering are reduced, the extraction effect is improved, and the extraction efficiency is improved. And the maintenance frequency of the filter barrel is also reduced.

Description

Technical Field

[0001] This application relates to the field of stem cell technology, and more specifically, to a device for extracting exosomes from canine and feline stem cells. Background Technology

[0002] Exosome extraction from canine and feline stem cells is the process of obtaining exosomes from stem cells in dogs and cats. Typically, tissue samples rich in stem cells, such as bone marrow and adipose tissue, are collected from dogs and cats first. Then, stem cells are obtained through enzymatic digestion and mechanical separation. The stem cell culture supernatant is then processed using techniques such as centrifugation and ultrafiltration, and finally, exosomes are filtered and screened out. These exosomes can be used for research and applications in canine and feline disease treatment and tissue repair.

[0003] In response, Chinese patent application number CN202222597937.4 discloses a filtration and extraction device for stem cell exosomes. This method mainly involves mixing the sample and diluent in a mixing chamber, and then pumping the mixture into a filtration chamber for filtration, thereby screening out exosomes.

[0004] However, in the process of implementing the technical solutions in the embodiments of this application, the inventors of this utility model discovered that the above-mentioned technology has at least the following technical problems:

[0005] The stirred liquid contains impurities such as cell debris and dead cells, which can easily clog the filter screen when it enters the filtration chamber, resulting in slow exosome extraction and reduced filter life. Utility Model Content

[0006] To overcome the above deficiencies, this application provides a canine and feline stem cell exosome extraction device, which aims to improve the problems mentioned in the background art.

[0007] This application provides a canine and feline stem cell exosome extraction device, including a transparent mixing tank and a filter barrel. The filter barrel has a rotating component at its opening, and the mixing tank rotates on the rotating component. A connecting pipe runs through the mixing tank and moves up and down. An overflow port is opened through the middle of the connecting pipe. The mixing tank is conditionally connected to the filter barrel through the connecting pipe.

[0008] In one specific implementation, the rotating component includes a bearing and a motor, the outer ring of the bearing is mounted on the upper end face of the filter barrel, the mixing tank is movably locked in the inner ring of the bearing, and the output end of the motor is poweredly connected to the mixing tank.

[0009] In the above process, a cross roller bearing is used. The mixing tank is clamped on the inner ring of the bearing and can rotate to achieve centrifugal action. It can also be lifted as a whole to expose the opening of the filter barrel, which facilitates maintenance inside the filter barrel.

[0010] In one specific embodiment, the rotating component further includes a gear and a gear ring, the gear being fixedly connected to the output end of the motor, and the gear ring being mounted on the mixing tank, with the gear meshing with the gear ring.

[0011] In the above process, the motor drives the mixing tank to rotate through the meshing of gears and gear rings to achieve centrifugal separation. When the mixing tank is unloaded, it can detach from the gears along with the gear rings.

[0012] In one specific implementation, a gripper cap is rotatably connected to the upper end of the connecting pipe.

[0013] In the above process, after centrifugation, the handheld gripper cap allows for easy control of the connecting tube's raising and lowering, ensuring the overflow port is at a suitable depth in the mixed liquid. This allows the relatively concentrated supernatant of exosomes to flow through the overflow port into the connecting tube, and then into the filter tank for filtration, thereby improving the filtration effect and reducing filter screen wear. On the other hand, the connecting tube can be raised and lowered while the tank is rotating at a low speed, and the handheld gripper cap will not obstruct the connecting tube from rotating with the tank, thus avoiding disturbing the already separated solutions.

[0014] In one specific implementation, the mixing tank includes a tank body and a tank cover, with the connecting pipe movably passing through the tank body and the tank cover.

[0015] In the above process, when the lid is placed on the tank body, the overflow port can rise to the lower part or even the upper part of the lid during centrifugal rotation, thereby preventing the solution from entering the overflow port.

[0016] In one specific implementation, a tray is fixedly connected to the lower end of the tank body, and both the tray and the tank cover are provided with sealing rings that abut against the outer circle of the connecting pipe. The tray rests on the bearing, and the gear ring is fixedly connected to the outer circle of the tray.

[0017] In the above implementation process, the tank is made of glass, the tray is used to support the tank, and the lower end of the tray is provided with a slot that can be locked onto the inner ring of the bearing and driven to rotate. It can also be removed like a bucket lid. The sealing ring can provide a sealing function and also achieve a damping function. When there is no external force acting on the connecting pipe, the connecting pipe can rotate with the tank.

[0018] In one specific embodiment, the mixing tank further includes a stirring blade, which is movably sleeved on the connecting pipe. A magnet is embedded in the outer circle of the connecting pipe and magnetically connected to the stirring blade. A protruding blade is fixedly connected to the inner wall of the tank.

[0019] In the above process, the tank is driven to rotate by a motor, and the solution is stirred by the convex blades to achieve centrifugal effect. Alternatively, before centrifugation, the connecting tube can be grasped and lifted to its maximum position. At this time, the overflow port is located on the top of the tank cover. The magnet on the connecting tube moves into the tank and magnetically attracts the stirring blade, lifting the stirring blade away from the bottom of the tank. The stirring blade is rotated by hand by rotating the connecting tube, thereby achieving the stirring effect. After stirring, the hand is released. Due to the damping effect of the sealing ring, the connecting tube and the stirring blade rotate together with the tank and the water flow, achieving the centrifugal effect. It should be noted that the magnets are neodymium magnets, and the circumferential array is on the outer circle of the connecting tube, with adjacent magnetic poles set in opposite directions. The corresponding stirring blades are also set in the same way, which can improve the circumferential fixation effect.

[0020] In one specific implementation, the filter barrel includes a barrel body and a filter screen, the outer ring of the bearing is fixedly connected to the opening of the barrel body, the filter screen is disposed on the inner wall of the barrel body, and a discharge pipe is provided at the lower end of the barrel body.

[0021] In the above process, the supernatant enters the filter screen, the exosomes pass through the filter screen, and are discharged through the discharge pipe.

[0022] Compared with the prior art, the beneficial effects of this application are: by raising and lowering the connecting tube, the overflow port is submerged at an appropriate depth in the solution, and the supernatant enriched with exosomes passes through the connecting tube from the overflow port into the filter bucket below for filtration, which reduces the participation of impurities such as cell debris and dead cells in filtration, improves the extraction effect, and also reduces the maintenance frequency of the filter bucket. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the external view of the canine and feline stem cell exosome extraction device provided in the embodiments of this application;

[0025] Figure 2 A cross-sectional view of the canine and feline stem cell exosome extraction device provided for an embodiment of this application;

[0026] Figure 3 A schematic diagram illustrating the connection relationship between the mixing tank and the connecting pipe provided for an embodiment of this application;

[0027] Figure 4 This is a schematic diagram of the filter bucket in the state of the mixing tank being removed, as provided in the embodiments of this application.

[0028] In the diagram: 10-mixing tank; 11-tank body; 12-tank lid; 13-tray; 14-stirring blade; 20-filter barrel; 21-barrel body; 22-filter screen; 30-rotating component; 31-bearing; 32-motor; 33-gear; 34-gear ring; 40-connecting pipe; 41-overflow port; 42-grab cap; 43-magnet. Detailed Implementation

[0029] The technical solutions in the embodiments of this application will now be described with reference to the accompanying drawings.

[0030] Please see Figures 1-4 This application provides a canine and feline stem cell exosome extraction device, including a transparent mixing tank 10 and a filter tank 20. A rotating component 30 is provided at the opening of the filter tank 20, and the mixing tank 10 rotates on the rotating component 30. A connecting pipe 40 extends and retracts through the mixing tank 10, and an overflow port 41 is provided through the middle of the connecting pipe 40. The mixing tank 10 is conditionally connected to the filter tank 20 through the connecting pipe 40. The rising and falling of the connecting pipe 40 allows the overflow port 41 to be submerged at an appropriate depth in the solution, allowing the supernatant enriched with exosomes to pass through the connecting pipe 40 from the overflow port 41 into the filter tank 20 below for filtration. This reduces the participation of impurities such as cell debris and dead cells in the filtration process, improves the extraction effect, and reduces the maintenance frequency of the filter tank 20.

[0031] Please see Figures 1-4 The rotating component 30 includes a bearing 31 and a motor 32. The outer ring of the bearing 31 is mounted on the upper end face of the filter barrel 20, and the mixing tank 10 is movably locked in the inner ring of the bearing 31. The output end of the motor 32 is connected to the mixing tank 10 for power. Using a crossed roller bearing 31, the mixing tank 10, locked in the inner ring of the bearing 31, can rotate to achieve centrifugal action, or it can be lifted as a whole, thus exposing the opening of the filter barrel 20 for easy maintenance of the interior of the filter barrel 20.

[0032] Please see Figures 1-4 The rotating component 30 also includes a gear 33 and a gear ring 34. The gear 33 is fixedly connected to the output end of the motor 32, and the gear ring 34 is mounted on the mixing tank 10, with the gear 33 meshing with the gear ring 34. The motor 32 drives the mixing tank 10 to rotate through the meshing of the gear 33 and the gear ring 34, achieving a centrifugal separation effect. When the mixing tank 10 is removed, it can detach from the gear 33 along with the gear ring 34.

[0033] Please see Figures 1-4A gripper cap 42 is rotatably connected to the upper end of the connecting tube 40. After centrifugation, the gripper cap 42 can be held to control the raising and lowering of the connecting tube 40, so that the overflow port 41 is at a suitable depth of the mixture. This allows the relatively concentrated supernatant of exosomes to flow through the overflow port 41 into the connecting tube 40, and then into the filter tank 20 for filtration, thereby improving the filtration effect and reducing the wear of the filter screen 22. On the other hand, the raising and lowering of the connecting tube 40 can be controlled while the tank 11 is rotating at a low speed. Holding the gripper cap 42 will not hinder the connecting tube 40 from rotating with the tank 11, thereby avoiding disturbing the already separated solutions.

[0034] Please see Figures 1-4 The mixing tank 10 includes a tank body 11 and a tank cover 12, with a connecting pipe 40 movably passing through the tank body 11 and the tank cover 12. The tank cover 12 covers the tank body 11. During centrifugation, the overflow port 41 can rise to the lower part of the tank cover 12 or even the upper part of the tank cover 12, thereby preventing the solution from entering the overflow port 41.

[0035] Please see Figures 1-4 A tray 13 is fixedly connected to the lower end of the tank body 11. Both the tray 13 and the tank lid 12 are equipped with sealing rings that abut against the outer circle of the connecting pipe 40. The tray 13 rests on the bearing 31, and the gear ring 34 is fixedly connected to the outer circle of the tray 13. The tank body 11 is made of glass. The tray 13 is used to support the tank body 11. The lower end of the tray 13 is provided with a slot that can be locked onto the inner ring of the bearing 31 and driven to rotate. It can also be removed like a bucket lid. The sealing ring can provide a sealing function and also achieve a damping function. When there is no external force acting on the connecting pipe 40, the connecting pipe 40 can rotate together with the tank body 11.

[0036] Please see Figures 1-4 The mixing tank 10 also includes a stirring blade 14, which is movably sleeved on the connecting pipe 40. A magnet 43 is embedded in the outer circle of the connecting pipe 40 and magnetically connected to the stirring blade 14. A protruding blade is fixedly connected to the inner wall of the tank body 11. The tank 11 is driven to rotate by the motor 32. The convex blades agitate the solution to achieve centrifugal action. Alternatively, before centrifugation, the connecting pipe 40 can be grasped and lifted to its maximum position. At this time, the overflow port 41 is located on the upper part of the tank cover 12. The magnet 43 on the connecting pipe 40 moves into the tank 11 and magnetically attracts the stirring blade 14, lifting the stirring blade 14 away from the bottom of the tank 11. The stirring blade 14 is rotated by hand by rotating the connecting pipe 40, thereby achieving the stirring effect. After stirring, the hand is released. Due to the damping effect of the sealing ring, the connecting pipe 40 and the stirring blade 14 rotate together with the tank 11 and the water flow, achieving the centrifugal action. It should be noted that the magnet 43 is a neodymium magnet, and the circumferential array is on the outer circle of the connecting pipe 40 with adjacent magnetic poles set in opposite directions. The corresponding stirring blade 14 is also set in the same way, which can improve the circumferential fixation effect.

[0037] Please see Figures 1-4The filter tank 20 includes a tank body 21 and a filter screen 22. The outer ring of the bearing 31 is fixedly connected to the opening of the tank body 21. The filter screen 22 is disposed on the inner wall of the tank body 21, and a discharge pipe is provided at the lower end of the tank body 21. The supernatant enters the filter screen 22, and the excrement passes through the filter screen 22 and is discharged through the discharge pipe. In this embodiment, the motor 32 is installed on the outer wall of the tank body 21.

[0038] The working principle of this canine and feline stem cell exosome extraction device is as follows: Stem cell samples and diluent are added to the tank 11. The motor 32 drives the tank 11 to rotate clockwise. The connecting tube 40 is rotated counterclockwise by hand to achieve a stirring effect. After releasing the hand, the connecting tube 40 and the stirring blade 14 rotate together with the tank 11 and the water flow, achieving centrifugation. After centrifugation, the exosomes are enriched in the supernatant. The connecting tube 40 is moved downwards, the stirring blade 14 separates from the magnet 43, and falls to the bottom of the tank 11. The overflow port 41 descends to an appropriate depth, allowing the supernatant to pass through the connecting tube 40 and enter the lower barrel 21 for filtration. Thus, only the supernatant is filtered. In summary, by raising and lowering the connecting tube 40, the overflow port 41 is submerged at an appropriate depth in the solution, allowing the supernatant enriched with exosomes to pass through the connecting tube 40 from the overflow port 41 into the lower filter barrel 20 for filtration. This reduces the participation of impurities such as cell debris and dead cells in filtration, improves the extraction effect, and reduces the maintenance frequency of the filter barrel 20.

[0039] The above are merely embodiments of this application and are not intended to limit the scope of protection of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, improvements, or equivalent substitutions made within the spirit and principles of this application should be included within the scope of protection of this application. It should be noted that similar reference numerals and letters in the following figures indicate similar items; therefore, once an item is defined in one figure, it does not need to be further defined and explained in subsequent figures.

Claims

1. A device for extracting exosomes from canine and feline stem cells, characterized in that, The device includes a transparent mixing tank (10) and a filter barrel (20). The filter barrel (20) has a rotating part (30) at its opening. The mixing tank (10) rotates on the rotating part (30). A connecting pipe (40) runs through the mixing tank (10) and moves up and down. An overflow port (41) is opened through the middle of the connecting pipe (40). The mixing tank (10) is conditionally connected to the filter barrel (20) through the connecting pipe (40).

2. The canine and feline stem cell exosome extraction device according to claim 1, characterized in that, The rotating component (30) includes a bearing (31) and a motor (32). The outer ring of the bearing (31) is mounted on the upper end face of the filter barrel (20). The mixing tank (10) is movably locked in the inner ring of the bearing (31). The output end of the motor (32) is poweredly connected to the mixing tank (10).

3. The canine and feline stem cell exosome extraction device according to claim 2, characterized in that, The rotating component (30) also includes a gear (33) and a gear ring (34). The gear (33) is fixedly connected to the output end of the motor (32), and the gear ring (34) is mounted on the mixing tank (10). The gear (33) meshes with the gear ring (34).

4. The canine and feline stem cell exosome extraction device according to claim 3, characterized in that, A gripper cap (42) is rotatably connected to the upper end of the connecting pipe (40).

5. The canine and feline stem cell exosome extraction device according to claim 4, characterized in that, The mixing tank (10) includes a tank body (11) and a tank cover (12), and the connecting pipe (40) extends through the tank body (11) and the tank cover (12).

6. The canine and feline stem cell exosome extraction device according to claim 5, characterized in that, The lower end of the tank (11) is fixedly connected to a tray (13). Both the tray (13) and the tank cover (12) are provided with sealing rings that abut against the outer circle of the connecting pipe (40). The tray (13) rests on the bearing (31), and the gear ring (34) is fixedly connected to the outer circle of the tray (13).

7. The canine and feline stem cell exosome extraction device according to claim 6, characterized in that, The mixing tank (10) also includes a stirring blade (14), which is movably sleeved on the connecting pipe (40). A magnet (43) is embedded in the outer circle of the connecting pipe (40) and magnetically connected to the stirring blade (14). A protruding blade is fixedly connected to the inner wall of the tank body (11).

8. The canine and feline stem cell exosome extraction device according to claim 7, characterized in that, The filter barrel (20) includes a barrel body (21) and a filter screen (22). The outer ring of the bearing (31) is fixedly connected to the opening of the barrel body (21). The filter screen (22) is disposed on the inner wall of the barrel body (21). A discharge pipe is provided at the lower end of the barrel body (21).

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