Filtering device for pretreatment of flow detection sample

Abstract

The utility model discloses a filtering device for flow type detection sample pretreatment, which comprises a base, electric telescopic rods are fixedly mounted on two sides of the top of the base, a filter cartridge is fixedly mounted on the surface of each electric telescopic rod through a support, and a pressing plate is fixedly mounted at the top end of each electric telescopic rod. A to-be-detected sample bottle is inserted into the filter cartridge, then the electric telescopic rod is controlled to retract, the electric telescopic rod retracts to drive the pressing plate to descend, the pressing plate can make contact with the bottom of the sample bottle after descending, and the sample bottle can be detected through the filter cartridge. At the moment, the effect that sample bottles with different sizes are in butt joint with the device can be achieved, the samples in the bottle bodies after butt joint can flow into the filter cartridge, the samples can be filtered through the filter plate at the moment, and the filtered samples can be discharged through the discharge pipe on the surface of the filter cartridge.

Description

Technical Field

[0001] This utility model relates to the field of flow cytometry sample processing, specifically a filtration device for pretreatment of flow cytometry samples. Background Technology

[0002] In biological and medical research, flow cytometry has become a key tool in cell analysis, disease diagnosis, and drug development due to its high efficiency, accuracy, and ability to perform multi-parameter analysis. Sample pretreatment is the foundation of this technical process, and its quality directly affects the accuracy of the test results. Among these, effectively filtering the sample and removing impurities, cell clumps, and other interfering substances to ensure that only single-cell suspensions enter the flow cytometer is crucial.

[0003] In actual scientific research and clinical testing, sample sources are diverse, and the bottles used to hold samples vary in size, from microcentrifuge tubes to ordinary test tubes and even specially customized containers. However, existing filtration devices for flow cytometry sample pretreatment face numerous difficulties when used with sample bottles of different sizes. Most devices are only compatible with specific sizes, requiring frequent replacement of compatible parts or even the entire device when dealing with sample bottles of other sizes. This is cumbersome and significantly reduces experimental efficiency. In addition, the filtration efficiency of existing filtration devices is also poor. Some devices have traditional principles and structures, resulting in slow sample filtration speeds that cannot meet the needs of high-throughput experiments. Filter clogging is also prone to occur during filtration, affecting the accuracy of test results. Therefore, it is necessary to design a filtration device for flow cytometry sample pretreatment to solve the above problems. Utility Model Content

[0004] The purpose of this invention is to provide a filtration device for sample pretreatment in flow cytometry to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a filtration device for pretreatment of samples for flow cytometry detection, comprising a base, electric telescopic rods fixedly installed on both sides of the top of the base, a filter cylinder fixedly installed on the surface of the electric telescopic rods via a bracket, a pressure plate fixedly installed at the top of the electric telescopic rods, and a filter plate fixedly installed on the inner wall of the filter cylinder;

[0006] A motor is fixedly installed on the top of the base through an opening. A reciprocating screw is fixedly installed on the output shaft of the motor through a coupling. A rotating rod is fixedly installed on the top of the reciprocating screw. The top of the rotating rod passes through the filter cylinder and the filter plate in sequence and extends to the outside of the filter plate. A scraper is fixedly installed on the top of the rotating rod.

[0007] Preferably, an airbag is fixedly installed on the right side of the filter cartridge via a bracket, a traction plate is fixedly installed at the bottom of the airbag, an air inlet pipe is connected to the bottom of the airbag, the bottom end of the air inlet pipe passes through the traction plate and extends to the outside of the traction plate, a first one-way valve is provided on the surface of the air inlet pipe, an air outlet pipe is connected to the top of the airbag, the left end of the air outlet pipe is connected to the right side of the filter cartridge, and a second one-way valve is provided on the surface of the air outlet pipe.

[0008] Preferably, the reciprocating screw is threadedly connected to a threaded sleeve, and an L-shaped rod is fixedly installed on the right side of the threaded sleeve. The top end of the L-shaped rod is fixedly connected to the bottom of the traction plate.

[0009] Preferably, a sliding plate is fixedly installed on the top of the base.

[0010] Preferably, the slide plate has a slider slidably connected inside.

[0011] Preferably, the right side of the slider is fixedly connected to the left side of the threaded sleeve.

[0012] Compared with the prior art, the beneficial effects of this utility model are:

[0013] 1. This filtration device for sample pretreatment in flow cytometry involves inserting the sample bottle to be tested into the filter cylinder, then controlling the retraction of the electric telescopic rod. The retraction of the electric telescopic rod causes the pressure plate to descend, and after the pressure plate descends, it can contact the bottom of the sample bottle. At this time, it can achieve the effect of docking sample bottles of different sizes with this device. After docking, the sample in the bottle can flow into the filter cylinder, where it can be filtered through the filter plate. The filtered sample can be discharged through the discharge pipe on the surface of the filter cylinder.

[0014] 2. This filtration device for pretreatment of samples for flow cytometry detection works by starting a motor, which drives a reciprocating screw and a rotating rod to rotate. The rotating rod then drives a scraper to rotate, preventing impurities from clogging the top of the filter plate. Simultaneously, the reciprocating screw drives a threaded sleeve to move up and down, which in turn moves an L-shaped rod. This L-shaped rod continuously delivers external gas into the filter cartridge through an outlet pipe, increasing the pressure inside the filter cartridge and thus improving filtration efficiency. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of this utility model;

[0016] Figure 2 This is a cross-sectional view of the base and filter cylinder structure of this utility model;

[0017] Figure 3 This is a schematic diagram of the reciprocating lead screw, threaded sleeve, and slider structure of this utility model.

[0018] In the diagram: 1. Base; 2. Electric telescopic rod; 3. Pressure plate; 4. Filter cylinder; 5. Filter plate; 6. Motor; 7. Reciprocating screw; 8. Rotating rod; 9. Scraper; 10. Airbag; 11. Traction plate; 12. Air inlet pipe; 13. First one-way valve; 14. Air outlet pipe; 15. Second one-way valve; 16. Threaded sleeve; 17. L-shaped rod; 18. Slide plate; 19. Slider. Detailed Implementation

[0019] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0020] Example 1

[0021] Please refer to Figure 1-3 As shown, this utility model provides a filtration device for pretreatment of samples for flow cytometry detection, including a base 1, an electric telescopic rod 2 fixedly installed on both sides of the top of the base 1, a filter cylinder 4 fixedly installed on the surface of the electric telescopic rod 2 by a bracket, a pressure plate 3 fixedly installed at the top of the electric telescopic rod 2, and a filter plate 5 fixedly installed on the inner wall of the filter cylinder 4.

[0022] Specifically, a motor 6 is fixedly installed on the top of the base 1 through an opening. The motor 6 is a servo motor. The output shaft of the motor 6 is fixedly installed with a reciprocating screw 7 through a coupling. A rotating rod 8 is fixedly installed at the top of the reciprocating screw 7. The top of the rotating rod 8 passes through the filter cylinder 4 and the filter plate 5 in sequence and extends to the outside of the filter plate 5. A scraper 9 is fixedly installed at the top of the rotating rod 8. By setting a pressure plate 3 and inserting the sample bottle to be tested into the filter cylinder 4, the electric telescopic rod 2 is controlled to retract. The retraction of the electric telescopic rod 2 drives the pressure plate 3 to descend. After the pressure plate 3 descends, it can contact the bottom of the sample bottle. At this time, it can achieve the effect of docking sample bottles of different sizes with this device. After docking, the sample in the bottle can flow into the filter cylinder 4, and the sample can be filtered through the filter plate 5.

[0023] Specifically, an airbag 10 is fixedly installed on the right side of the filter cartridge 4 via a bracket. A traction plate 11 is fixedly installed at the bottom of the airbag 10. An air inlet pipe 12 is connected to the bottom of the airbag 10. The bottom end of the air inlet pipe 12 passes through the traction plate 11 and extends to the outside of the traction plate 11. A first one-way valve 13 is provided on the surface of the air inlet pipe 12. An air outlet pipe 14 is connected to the top of the airbag 10. The left end of the air outlet pipe 14 is connected to the right side of the filter cartridge 4. A second one-way valve 15 is provided on the surface of the air outlet pipe 14. A threaded sleeve 16 is threadedly connected to the surface of the reciprocating screw 7. An L-shaped rod 17 is fixedly installed on the right side of the threaded sleeve 16. The top end of the L-shaped rod 17 is fixedly connected to the bottom of the traction plate 11. The base 1... A sliding plate 18 is fixedly installed at the top, and a slider 19 is slidably connected inside the sliding plate 18. The right side of the slider 19 is fixedly connected to the left side of the threaded sleeve 16. By setting a motor 6 and starting the motor 6, the motor 6 drives the reciprocating screw 7 and the rotating rod 8 to rotate. The rotation of the rotating rod 8 drives the scraper 9 to rotate. The rotation of the scraper 9 can prevent impurities from clogging the top of the filter plate 5. At the same time, the rotation of the reciprocating screw 7 can drive the threaded sleeve 16 to move up and down. The movement of the threaded sleeve 16 drives the L-shaped rod 17 to move. The movement of the L-shaped rod 17 can continuously transport external gas into the filter cylinder 4 through the air outlet pipe 14. At this time, the pressure inside the filter cylinder 4 increases, thereby improving the filtration efficiency.

[0024] Working Principle: This utility model is a filtration device for pretreatment of samples in flow cytometry. The sample bottle to be tested is inserted into the filter cylinder 4. Then, the electric telescopic rod 2 is controlled to retract, causing the pressure plate 3 to descend. After descending, the pressure plate 3 contacts the bottom of the sample bottle, achieving the effect of docking sample bottles of different sizes with the device. After docking, the sample inside the bottle flows into the filter cylinder 4, where it is filtered through the filter plate 5. The filtered sample is discharged through the discharge pipe on the surface of the filter cylinder 4. During the filtration process, the motor 6 can be started, driving the reciprocating screw 7 and the rotating rod 8 to rotate. The rotating rod 8 drives the scraper 9 to rotate, preventing impurities from clogging the top of the filter plate 5. Simultaneously, the reciprocating screw 7 drives the threaded sleeve 16 to move up and down reciprocally. The movement of the threaded sleeve 16 drives the L-shaped rod 17 to move, continuously supplying external gas into the filter cylinder 4 through the outlet pipe 14. This increases the pressure inside the filter cylinder 4, improving filtration efficiency.

[0025] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0026] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A filtration device for sample pretreatment in flow cytometry, comprising a base (1), characterized in that: Electric telescopic rods (2) are fixedly installed on both sides of the top of the base (1). A filter cylinder (4) is fixedly installed on the surface of the electric telescopic rod (2) through a bracket. A pressure plate (3) is fixedly installed at the top of the electric telescopic rod (2). A filter plate (5) is fixedly installed on the inner wall of the filter cylinder (4). A motor (6) is fixedly installed on the top of the base (1) through an opening. A reciprocating screw (7) is fixedly installed on the output shaft of the motor (6) through a coupling. A rotating rod (8) is fixedly installed on the top of the reciprocating screw (7). The top of the rotating rod (8) passes through the filter cylinder (4) and the filter plate (5) in sequence and extends to the outside of the filter plate (5). A scraper (9) is fixedly installed on the top of the rotating rod (8).

2. The filtration device for sample pretreatment in flow cytometry detection according to claim 1, characterized in that: An airbag (10) is fixedly installed on the right side of the filter cartridge (4) by a bracket. A traction plate (11) is fixedly installed at the bottom of the airbag (10). An air inlet pipe (12) is connected to the bottom of the airbag (10). The bottom end of the air inlet pipe (12) passes through the traction plate (11) and extends to the outside of the traction plate (11). A first one-way valve (13) is provided on the surface of the air inlet pipe (12). An air outlet pipe (14) is connected to the top of the airbag (10). The left end of the air outlet pipe (14) is connected to the right side of the filter cartridge (4). A second one-way valve (15) is provided on the surface of the air outlet pipe (14).

3. The filtration device for sample pretreatment in flow cytometry detection according to claim 2, characterized in that: The reciprocating screw (7) is threaded with a threaded sleeve (16), and an L-shaped rod (17) is fixedly installed on the right side of the threaded sleeve (16). The top end of the L-shaped rod (17) is fixedly connected to the bottom of the traction plate (11).

4. A filtration device for sample pretreatment in flow cytometry according to claim 3, characterized in that: A sliding plate (18) is fixedly installed on the top of the base (1).

5. A filtration device for sample pretreatment in flow cytometry according to claim 4, characterized in that: The slide plate (18) is internally connected to a slider (19).

6. A filtration device for sample pretreatment in flow cytometry according to claim 5, characterized in that: The right side of the slider (19) is fixedly connected to the left side of the threaded sleeve (16).

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