Optical tweezers optical path module and cell optimization system

Abstract

The utility model belongs to the technical field of optical instruments, and particularly relates to an optical tweezer optical path module and a cell optimization system. The optical tweezer optical path module comprises a supporting base, a laser head mounting seat and a multi-stage attenuation module. The laser head mounting seat is arranged on the supporting base and is used for mounting a laser emitting head for emitting laser; and the multi-stage attenuation module is arranged on the supporting base and comprises a plurality of reflection attenuation mirror groups which are arranged on the light path at intervals, and each reflection attenuation mirror group is used for attenuating the laser intensity and changing the laser direction. The optical tweezer optical path module provided by the utility model has a multi-attenuation mechanism, and a relatively large attenuation multiple range is easier to realize, so that laser power adjustment in a relatively large range is realized, the laser power dynamic adjustment capability is favorably improved, the laser intensity is finely controlled, and cells are not damaged when various cells are fixed through an optical trap formed by laser.

Description

Technical Field

[0001] This application belongs to the field of optical instrument technology, and in particular relates to an optical tweezers optical path module and a cell selection system. Background Technology

[0002] Optical tweezers, as a non-contact micromanipulation technique, exhibits unique advantages in the manipulation of microscopic particles. Its core relies on a highly focused laser beam forming an optical potential trap to capture target particles. However, in applications involving specialized / rare cells (such as sperm cells), the precise control of laser intensity directly determines the safety and effectiveness of the experiment.

[0003] Currently, traditional optical tweezers systems have significant limitations in laser intensity control, especially the lack of a multi-level attenuation mechanism, which limits the range of laser intensity adjustment and results in insufficient dynamic adjustment capability, leading to a high risk of damage to microscopic organisms. Utility Model Content

[0004] This application provides an optical tweezers optical path module and a cell optimization system to solve the technical problem in the prior art that the laser intensity adjustment range is limited and the dynamic adjustment capability is insufficient, resulting in a high risk of damage to microscopic organisms.

[0005] The first aspect of this application provides an optical tweezers optical path module, including a support base, a laser head mounting base, and a multi-stage attenuation module. The laser head mounting base is disposed on the support base and is used to mount a laser emitting head that emits laser light; the multi-stage attenuation module is disposed on the support base and includes multiple reflective attenuation mirror groups arranged at intervals along the optical path, each reflective attenuation mirror group being used to attenuate the laser intensity and change the laser direction.

[0006] In an optional embodiment of this application, the multi-stage attenuation module further includes an optical path adjustment attenuation mirror group, which is used to change the laser beam diameter and direction and is arranged at intervals with each reflection attenuation mirror group in the optical path.

[0007] In the optional scheme of this application, the multiple reflection attenuation mirror groups include a first-level reflection attenuation mirror group, a second-level reflection attenuation mirror group, and a third-level reflection attenuation mirror group; the first-level reflection attenuation mirror group, the second-level reflection attenuation mirror group, the optical path adjustment attenuation mirror group, and the third-level reflection attenuation mirror group are arranged sequentially at intervals in the optical path direction, and the first-level reflection attenuation mirror group is close to the laser head mounting base in the optical path direction.

[0008] In an optional embodiment of this application, the optical path adjustment attenuation mirror group includes a first aperture, a second aperture, and a beam expander unit; the first aperture and the second aperture are located upstream and downstream of the beam expander unit, respectively, in the optical path direction.

[0009] In an optional embodiment of this application, the beam expander unit includes a beam expander, a front upright plate, a rear upright plate, a movable block, a first adjusting rod group, and a second adjusting rod group. The front upright plate and the rear upright plate are arranged at intervals in the optical path and are connected by the first adjusting rod group. The rear upright plate has a central through hole, the movable block is disposed in the central through hole and is connected to the movable block by the second adjusting rod group, and the beam expander is connected to the movable block. The first adjusting rod group is used to cause the rear upright plate to drive the front upright plate and the beam expander to swing relative to the front upright plate together, and the second adjusting rod group is used to cause the movable block to drive the beam expander to move.

[0010] In an optional embodiment of this application, the multi-stage attenuation module further includes a reflector, which is located in the optical path and between the optical path adjustment attenuation mirror group and the three-stage reflection attenuation mirror group.

[0011] In an optional embodiment of this application, a shutter is also provided on the support base, which is located downstream of the third-order reflective attenuator group in the optical path direction; the shutter, laser head mount, and first-order reflective attenuator group are arranged sequentially at intervals along the length of the support base to form a first column; the reflector, optical path adjustment attenuator group, and second-order reflective attenuator group are arranged sequentially at intervals along the length of the support base to form a second column; the first column and the second column are arranged at intervals along the width of the support base, and the third-order reflective attenuator group is located above the reflector and aligned with the shutter in the height direction of the support base.

[0012] In an optional embodiment of this application, the support base includes a support base plate, a first mounting plate, a second mounting plate, a reflector frame, an output mirror stand, and a shutter stand. The first mounting plate, second mounting plate, reflector frame, output mirror stand, and shutter stand are all detachably connected to the support base plate. The first mounting plate and reflector frame are configured to have adjustable mounting positions along the length of the support base, and the second mounting plate and output mirror stand are configured to have adjustable mounting positions along the width of the support base. A first-stage attenuating filter assembly is detachably connected to the first mounting plate and is configured to have adjustable relative angles with the first mounting plate. A second-stage attenuating filter assembly is detachably connected to the second mounting plate and is configured to have adjustable relative angles with the second mounting plate. A third-stage attenuating filter assembly is detachably connected to the output mirror stand and is configured to have adjustable mounting positions along the height of the support base and relative angles with the output mirror stand. The shutter stand is equipped with a shutter mounting bracket, which is used to mount the shutter and is configured to have adjustable mounting positions along the length of the support base.

[0013] In an optional embodiment of this application, the reflective attenuation lens assembly includes a lens, an aperture plate, a mating plate, and multiple adjusting rods; the lens is disposed on the aperture plate, the aperture plate and the mating plate are arranged at intervals and connected by multiple adjusting rods, and the multiple adjusting rods can act on the aperture plate to allow the aperture plate to drive the lens to swing relative to the mating plate.

[0014] A second aspect of this application provides an optical tweezers optical path module, including an optical microscopy device and the aforementioned optical tweezers optical path module, wherein the optical microscopy device is located downstream of the optical tweezers optical path module in the optical path direction.

[0015] Compared with the prior art, this application has the following beneficial effects:

[0016] The optical tweezers optical path module provided in this application has at least a support base, a laser head mounting base, and a multi-stage attenuation module. The support base provides support for each component in the optical tweezers optical path module, and the laser head mounting base is used to fix the laser head that can emit laser light as a laser source.

[0017] The multi-stage attenuation module has at least multiple reflective attenuation mirror groups. These reflective attenuation mirror groups can emit lasers to change the laser direction and attenuate the laser intensity, achieving multiple attenuation of the laser intensity. The multiple attenuation mechanism makes it easier to achieve a larger attenuation factor range, thereby achieving a wider range of laser power adjustment. This is beneficial for improving the dynamic adjustment capability of laser power, achieving fine control of laser intensity, and reducing the risk of damage to microscopic organisms caused by excessive laser intensity.

[0018] Accordingly, the cell selection system equipped with this optical tweezers circuit module can effectively reduce the laser intensity so as not to damage the cells when fixing them through the laser spot. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the specific embodiments of this application or the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of an optical tweezers optical path module provided according to one embodiment of this application;

[0021] Figure 2 for Figure 1 A magnified view of the locations of the primary and secondary reflection attenuation mirror groups in the image;

[0022] Figure 3 for Figure 1 A magnified view of the location of the optical path adjustment attenuation mirror group in the image;

[0023] Figure 4 for Figure 1 A magnified view of the middle three-stage reflective attenuation lens group, the reflector, and the location of the shutter.

[0024] Figure Labels

[0025] 100. Optical tweezers optical path module;

[0026] 10. Support base; 11. Support base plate; 12. First mounting plate; 13. Second mounting plate; 14. Reflector frame; 15. Output mirror stand plate; 16. Shutter stand plate; 161. Shutter mounting bracket; H1. Mating hole; H2, H3, H4, H5, H6. Elongated holes; C1, C2. Elongated openings;

[0027] 20. Laser head mounting base;

[0028] 30. Multi-stage attenuation module; 31. Reflection attenuation mirror assembly; 3101. Lens; 3102. Opening plate; 3103. Fitting plate; 3104. Adjusting rod; 3105. Pin;

[0029] 311. First-order attenuating mirror group; 312. Second-order attenuating mirror group; 313. Third-order attenuating mirror group

[0030] 32. Optical path adjustment attenuation lens group; 321. First aperture stop; 322. Second aperture stop; 323. Beam expander unit; 3231. Beam expander; 3232. Front plate; 3233. Rear plate; 3234. Movable block; 3235. First adjustment rod group; 3236. Second adjustment rod group;

[0031] 33. Reflector;

[0032] 40. Shutter speed. Detailed Implementation

[0033] To make the above and other features and advantages of this application clearer, the application is further described below with reference to the accompanying drawings. It should be understood that the specific embodiments given herein are for the purpose of explanation to those skilled in the art and are exemplary only, not restrictive.

[0034] Figure 1 This is a schematic diagram of an optical tweezers optical path module 100 according to one embodiment of this application. Please refer to... Figure 1 The optical tweezers optical path module 100 includes a support base 10, a laser head mounting base 20, and a multi-stage attenuation module 30.

[0035] A laser head mounting base 20 is disposed on a support base 10 and is used to mount a laser emitting head that emits laser light. A multi-stage attenuation module 30 is disposed on the support base 10 and includes multiple reflective attenuation mirror groups 31 spaced apart in the optical path. Each reflective attenuation mirror group 31 is used to attenuate the laser intensity and change the laser direction.

[0036] In this embodiment, the support base 10 provides support for the laser head mounting base 20 and the multi-stage attenuation module 30, serving as the main load-bearing component. It should be noted that the laser emitting head (not shown in the figure) is a component of the laser, from which the laser beam is emitted. In this specific embodiment, a fiber laser is used; therefore, the laser emitting head is a fiber laser head. However, the laser is not limited to fiber lasers and can also be a femtosecond laser, etc.

[0037] The laser emitter head of the laser is fixed by the laser head mounting base 20 to prevent the laser emitter head from shaking. The multi-stage attenuation module 30 has at least multiple reflective attenuation mirror groups 31, which have the functions of attenuating laser intensity and changing laser direction. Here, laser intensity can refer to laser power.

[0038] The laser emitted from the laser emitter is incident on the multi-stage attenuation module 30, and under the action of multiple reflection attenuation mirror groups 31, the optical path direction can be changed multiple times before the laser reaches the target position. Moreover, after the laser passes through multiple reflection attenuation mirror groups 31, the laser intensity is attenuated multiple times, allowing the laser intensity to reach the target intensity.

[0039] Understandably, multiple attenuation mechanisms make it easier to achieve a larger range of attenuation factors, thereby enabling a wider range of laser power adjustment. This is beneficial for improving the dynamic adjustment capability of laser power, achieving precise control of laser intensity, and reducing the risk of damage to microscopic organisms due to excessive laser intensity.

[0040] As a further preferred embodiment, based on the above-described scheme, the specific embodiments of this application may also include one or more of the following additions or combinations of embodiments.

[0041] Figure 2 for Figure 1 A magnified view of the locations of the first-stage attenuating mirror group 311 and the second-stage attenuating mirror group 312. (See also...) Figure 2 In some optional embodiments, the reflective attenuation mirror assembly 31 includes a lens 3101, an opening plate 3102, a mating plate 3103, and a plurality of adjusting rods 3104.

[0042] Lens 3101 is disposed on opening plate 3102. Opening plate 3102 and mating plate 3103 are arranged at intervals and connected by multiple adjusting rods 3104. Multiple adjusting rods 3104 can act on opening plate 3102 to make opening plate 3102 drive lens 3101 to swing relative to mating plate 3103.

[0043] In this embodiment, an opening is formed on the opening plate 3102 for mounting the lens 3101. The mating plate 3103 is fixed in position. The mating plate 3103 and the opening plate 3102 are movably connected by multiple adjusting rods 3104. By adjusting the screw-in depth of each adjusting rod 3104, the opening plate 3102 can be swung relative to the mating plate 3103, thereby changing the angle between the lens 3101 and the laser, i.e., the incident angle of the laser.

[0044] In the illustrated embodiment, the adjusting rod 3104 can be a screw, set screw, etc., and there are two of them. The lens 3101 is a beam splitter. The beam splitter can transmit part of the light beam and reflect part of the light beam, thereby achieving the purpose of attenuating the light beam intensity. The mating plate 3103 also forms a larger opening than the opening plate 3102 to allow the light beam transmitted through the beam splitter to pass through.

[0045] Two adjusting rods 3104 are located at both ends of the opening of the mating plate 3103, and the mating plate 3103 and the corner of the opening plate 3102 are connected by a pin 3105. By rotating the adjusting rods at both ends of the opening, the opening plate 3102 can swing based on the pin 3105.

[0046] It should be noted that a beam splitter is used here to reflect and attenuate the laser intensity, and the laser intensity can be evaluated by collecting the laser transmitted through the beam splitter. Of course, the lens 3101 is not limited to a beam splitter; for example, a reflective attenuator can also be used to absorb some light energy.

[0047] In specific applications, the acute angle formed between the lens 3101 in the reflection attenuation mirror group 31 and the laser beam is 30° to 60°, preferably 45°.

[0048] In some optional embodiments, the multi-stage attenuation module 30 further includes an optical path adjustment attenuation mirror group 32 for changing the laser beam diameter and direction, and is arranged at intervals with each reflection attenuation mirror group 31 in the optical path.

[0049] In this embodiment, the optical path adjustment attenuation mirror group 32 can not only change the laser beam diameter, but also attenuate the laser intensity. The multi-stage attenuation module 30 can further increase the number of laser intensity attenuation times and adjust the laser beam diameter through the optical path adjustment attenuation mirror group 32. Here, the laser beam diameter refers to the diameter of the laser beam.

[0050] In a further optional embodiment, the plurality of reflection attenuation mirror groups 31 include a first-level reflection attenuation mirror group 311, a second-level reflection attenuation mirror group 312, and a third-level reflection attenuation mirror group 313.

[0051] The first-stage reflection attenuator group 311, the second-stage reflection attenuator group 312, the optical path adjustment attenuator group 32, and the third-stage reflection attenuator group 313 are arranged sequentially at intervals in the optical path direction, and the first-stage reflection attenuator group 311 is close to the laser head mounting base 20 in the optical path direction.

[0052] In this embodiment, there are three reflective attenuation mirror groups 31: a first-order reflective attenuation mirror group 311, a second-order reflective attenuation mirror group 312, and a third-order reflective attenuation mirror group 313. These three reflective attenuation mirror groups 31 can change the optical path direction three times and achieve three-fold attenuation of the laser light intensity. That is, after the laser is redirected by any reflective attenuation mirror group 31, its power is reduced accordingly. Moreover, the attenuation factors of these three reflective attenuation mirror groups 31 can be the same or different, and can be adjusted according to requirements.

[0053] It should be understood that the laser emitting head, as the light source of the optical path, is at the upstream end in the optical path direction. Downstream of the laser reflecting head are the first-level reflection attenuation mirror group 311, the second-level reflection attenuation mirror group 312, the optical path adjustment attenuation mirror group 32, and the third-level reflection attenuation mirror group 313, which are arranged in sequence at intervals.

[0054] The optical path adjustment attenuation mirror group 32 is located between the second-order reflection attenuation mirror group 312 and the third-order reflection attenuation mirror group 313. In this way, the laser is attenuated twice before entering the optical path adjustment attenuation mirror group 32, which helps to improve the service life of the optical path adjustment attenuation mirror group 32.

[0055] In a further optional embodiment, the optical path adjustment attenuation mirror group 32 includes a first aperture 321, a second aperture 322, and a beam expander unit 323. The first aperture 321 and the second aperture 322 are located upstream and downstream of the beam expander unit 323, respectively, in the optical path direction.

[0056] In this embodiment, the laser beam and laser intensity are changed by the cooperation of the aperture and the beam expander unit 323. It can be understood that the beam expander unit 323 is an optical device composed of optical lenses and mechanical structures, which can change the laser beam diameter and is generally used to increase the laser beam diameter. The aperture is provided with an aperture hole, which is a micro-aperture, and can also limit the laser beam diameter.

[0057] Specifically, the edge of the laser beam reflected by the secondary reflective attenuation mirror group 312 is blocked by the first aperture 321, allowing only the central part of the beam with better beam quality to pass through. Understandably, the edge of the laser beam is blocked, thus reducing the laser intensity and reducing the beam diameter, thereby achieving the diameter limiting effect.

[0058] After the laser passes through the first aperture 321, the laser beam diameter is expanded by the beam expander unit 323, and the laser energy is dispersed. However, when it passes through the aperture of the second aperture 322, the center part of the beam with better beam quality passes through while the edges are blocked, which plays the role of limiting the diameter and reducing the laser intensity.

[0059] It can be seen that by combining the beam expander unit 323 with the two apertures, a light spot with better quality, lower intensity, smaller beam diameter, and better shape can be obtained.

[0060] Figure 3 for Figure 1 A magnified view of the location of the optical path adjustment attenuation mirror group. Please refer to [link / reference]. Figure 3 In a further optional embodiment, the beam expander unit 323 includes a beam expander 3231, a front upright plate 3232, a rear upright plate 3233, a movable block 3234, a first adjusting rod group 3235, and a second adjusting rod group 3236. The front upright plate 3232 and the rear upright plate 3233 are arranged at a distance in the optical path and are connected by the first adjusting rod group 3235.

[0061] The rear upright plate 3233 has a central through hole, the movable block 3234 is set in the central through hole and is connected to the movable block 3234 through the second adjusting rod group 3236, and the beam expander 3231 is connected to the movable block 3234.

[0062] The first adjusting rod group 3235 is used to cause the rear upright plate 3233 to drive the front upright plate 3232 and the beam expander 3231 to swing relative to the front upright plate 3232 together. The second adjusting rod group 3236 is used to cause the movable block 3234 to drive the beam expander 3231 to move.

[0063] In this embodiment, the beam expander 3231 can be a combination of lenses, such as a combination of concave and convex lenses, to adjust the size of the laser beam by adjusting the divergence angle of the laser beam.

[0064] The front upright plate 3232 and the rear upright plate 3233 are arranged at intervals in the optical path direction, and the front upright plate 3232 is located upstream of the rear upright plate 3233 in the optical path direction. The two are movably connected by the first adjusting rod group 3235.

[0065] In one optional embodiment, both the front upright plate 3232 and the rear upright plate 3233 are generally rectangular. The first adjusting rod assembly 3235 consists of multiple set screws, specifically two set screws, connected diagonally opposite each other on the front upright plate 3232 and the rear upright plate 3233. Screwing in a set screw at one corner and unscrewing the set screw at the other corner allows the rear upright plate 3233 to swing relative to the front upright plate 3232. Of course, the first adjusting rod assembly 3235 is not limited to a combination of multiple set screws; it can also consist of multiple screws, bolts, etc.

[0066] Furthermore, the rear upright plate 3233 has a central through hole, the movable block 3234 is embedded in the central through hole, and is movably connected to the rear upright plate 3233 through the second adjusting rod group 3236.

[0067] In one optional embodiment, both the rear upright plate 3233 and the movable block 3234 are generally rectangular, and the central through hole on the rear upright plate 3233 is also rectangular. The second adjusting rod group 3236 consists of multiple set screws, and the number of set screws is four. These four set screws are located on the four sides of the rear upright plate 3233 and the movable block 3234, and are arranged opposite to each other in pairs.

[0068] It should be understood that the position of the movable block 3234 within the central through hole can be adjusted in the direction of the two opposing set screws. Of course, the second adjusting rod assembly 3236 is not limited to a combination of multiple set screws, but can also be a combination of multiple screws, bolts, etc.

[0069] Furthermore, since the beam expander 3231 is mounted on the movable block 3234, the beam expander 3231 can move with the movable block 3234 and subsequently the upright plate 3233 can swing to adjust the optical path.

[0070] It should be noted that the optical path adjustment attenuation mirror group 32 is not limited to the combination of a beam expander and an aperture. A prism can also be used to increase the beam diameter. That is, the optical path adjustment attenuation mirror group 32 can also be a combination of a prism and an aperture.

[0071] Figure 4 for Figure 1 A magnified view of the middle three-stage attenuation filter group, the mirror, and the location of the shutter. (Please refer to...) Figure 4 In some optional embodiments, the multi-stage attenuation module 30 further includes a reflector 33, which is located in the optical path and between the optical path adjustment attenuation mirror group 32 and the tertiary reflection attenuation mirror group 313.

[0072] In this embodiment, the reflector 33 only serves to adjust the direction of the laser and does not attenuate the laser, so that the laser is incident on the third-order reflective attenuation mirror group 313 in the required direction.

[0073] In the illustrated embodiment, the acute angle formed by the reflector 33 and the laser beam is 45°, but it is not limited to this and can be adjusted according to requirements. Specifically, the acute angle formed by the reflector 33 and the laser beam is 30° to 60°.

[0074] In a further optional embodiment, the optical tweezers optical path module 100 also includes a shutter 40 disposed on the support base 10, the shutter 40 being located downstream of the third-order reflection attenuation mirror group 313 in the optical path direction.

[0075] The shutter 40, laser head mounting base 20, and first-stage reflective attenuator assembly 311 are arranged at intervals along the length of the support base 10, forming the first column.

[0076] The reflector 33, the optical path adjustment attenuation mirror group 32, and the first-stage reflection attenuation mirror group 312 are arranged at intervals along the length of the support base 10, forming a second column.

[0077] The first and second columns are arranged at intervals in the width direction of the support base 10, and the three-stage reflective attenuation mirror group 313 is located above the reflector 33 and aligned with the shutter 40 in the height direction of the support base 10.

[0078] In this embodiment, the shutter 40 is used to control the on / off of the optical path of the downstream optical device that cooperates with the optical tweezers optical path module 100, and the laser reflected by the third-level reflection attenuation mirror group 313 can be emitted through the shutter 40 in the open state.

[0079] The laser emitted from the laser head at the laser head mounting base 20 first enters the first-stage reflective attenuator group 311 along the length direction L, then changes direction and enters the second-stage reflective attenuator group 312 along the width direction W, then changes direction again and enters the optical path adjustment attenuator group 32 along the length direction L, then enters the third-stage reflective attenuator group 313 along the height direction H after being changed direction by the reflector 33, then changes direction and enters the shutter 40 along the width direction W.

[0080] It can be seen that by reasonably arranging the positions of each lens group on the support base 10, the optical path is extended in a roundabout way, which can reduce the space occupied by the optical tweezers optical path module 100.

[0081] In a further optional embodiment, the support base 10 includes a support base plate 11, a first mounting plate 12, a second mounting plate 13, a reflector frame 14, an output mirror stand 15, and a shutter stand 16.

[0082] The first mounting plate 12, the second mounting plate 13, the reflector frame 14, the output mirror stand 15, and the shutter stand 16 are all detachably connected to the support base plate 11. The first mounting plate 12 and the reflector frame 14 are configured to be able to adjust their installation positions in the length direction of the support base 10, and the second mounting plate 13 and the output mirror stand 15 are configured to be able to adjust their installation positions in the width direction of the support base 10.

[0083] The first-stage reflective attenuator assembly 311 is detachably connected to the first mounting plate 12 and is configured to adjust its relative angle with the first mounting plate 12; the second-stage reflective attenuator assembly 312 is detachably connected to the second mounting plate 13 and is configured to adjust its relative angle with the second mounting plate 13.

[0084] The three-stage reflection attenuation mirror assembly 313 is detachably connected to the output mirror stand plate 15 and is configured to be able to adjust the installation position in the height direction of the support base 10 and the relative angle with the output mirror stand plate 15.

[0085] The shutter stand 16 is provided with a shutter mounting bracket 161, which is used to mount the shutter 40 and is configured to be able to adjust the mounting position in the length direction of the support base 10.

[0086] In this embodiment, the support base 10 is composed of multiple sheet metal parts, wherein the support base plate 11 provides support for the other sheet metal parts. The length direction L, width direction W and height direction H of the support base 10 mentioned above also refer to the length direction, width direction and thickness direction of the support base plate 11.

[0087] The first mounting plate 12 and the second mounting plate 13 are detachably coupled to the first-stage reflective attenuator assembly 311 and the second-stage reflective attenuator assembly 312, respectively. In specific applications, the mating plate 3103 in the reflective attenuator assembly 31 has a mating hole H1 in the middle and is coupled to the corresponding mounting plate by screws. The reflective attenuator assembly 31 can be locked onto the corresponding mounting plate by screwing in the screws. When the screws are loosened, the reflective attenuator assembly 31 can rotate around the screws to adjust its relative angle with the corresponding mounting plate, which mainly realizes the coarse adjustment of the incident angle. The fine adjustment of the incident angle is realized by the adjusting rod 3104 on the reflective attenuator assembly 31.

[0088] Of course, the fit between the reflective attenuator assembly 31 and the mounting plate is not limited to the detachable fit achieved by screws, which also allows for adjustment of the mounting angle. It can also be achieved by means of rotating or pulling pins.

[0089] The first mounting plate 12 and the second mounting plate 13 have the same structure. Both of them have elongated openings in the length direction. The elongated opening C1 on the first mounting plate 12 is arranged along the length direction L, and the elongated opening C2 on the second mounting plate 13 is arranged along the width direction W.

[0090] In practical applications, these elongated openings can be used with bolts to detachably install the mounting plate onto the support base plate 11. Adjusting the position of the bolts at the elongated openings can change the installation position of the mounting plate, thereby enabling the first mounting plate 12 to adjust its installation position in the length direction L, and the second mounting plate 13 to adjust its installation position in the width direction W.

[0091] In addition, the reflector frame 14 is used to install the reflector 33, and its bottom is provided with an elongated hole H2 extending along the length direction L. It is detachably installed on the support base plate 11 by bolts. By adjusting the installation position of the bolts in the elongated hole H2, the installation position of the reflector frame 14 in the length direction L can be adjusted.

[0092] Correspondingly, the shutter mount 161 also has an elongated hole H3 extending along the length direction L, which can be detachably connected with bolts and its installation position can be adjusted along the length direction L. Similarly, the bottom of the output lens stand plate 15 has an elongated hole H4 extending along the width direction W, and the bottom of the front stand plate 3232 of the beam expander 3231 also has an elongated hole H5 extending along the width direction W. Both can be detachably connected with bolts and their installation positions can be adjusted along the width direction W.

[0093] The three-stage reflection attenuator assembly 313 serves as an output mirror assembly and is mounted on the output mirror stand 15. The top of the output mirror stand 15 is provided with an elongated hole H6 extending along the height direction H. The three-stage reflection attenuator assembly 313 can be detachably installed with bolts. Furthermore, the three-stage reflection attenuator assembly 313 can be rotated and moved when loosened, that is, the installation height of the three-stage reflection attenuator assembly 313 and its relative angle with the output mirror stand 15 can be adjusted.

[0094] As can be seen, the positions of each mirror group in the multi-stage attenuation module 30 are adjustable, which makes it easier to correct the optical path.

[0095] It should be noted that the design is not limited to setting elongated holes on each mating plate to accommodate bolts for position adjustment. For example, position adjustment can also be achieved through a slider groove with locking function. In addition, the optical tweezers optical path module 100 also includes a cover (not shown in the figure), which is connected to the support base 10 to protect the optical components on the support base 10.

[0096] Another aspect of this application provides a cell optimization system, including an optical microscope and the aforementioned optical tweezers optical path module 100, wherein the optical microscope is located downstream of the optical tweezers optical path module 100 in the optical path direction. In some optional embodiments, the laser entering the optical microscope can be switched on or off by switching the shutter 40 in the optical tweezers optical path module 100.

[0097] It should be understood that the cell selection system possesses all the advantages of the aforementioned optical tweezers optical path module 100, and has a wide range of laser power adjustment, which is conducive to improving the dynamic adjustment capability of laser power so as not to damage cells when fixing cells through the laser spot.

[0098] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. An optical tweezers optical path module, characterized in that, include: Support base (10); A laser head mounting base (20) is disposed on the support base (10) and used to mount a laser emitting head that emits laser light; as well as A multi-stage attenuation module (30) is disposed on the support base (10) and includes multiple reflection attenuation mirror groups (31) arranged at intervals in the optical path. Each reflection attenuation mirror group (31) is used to attenuate the laser intensity and change the laser direction.

2. The optical tweezers optical path module according to claim 1, characterized in that, The multi-stage attenuation module (30) further includes an optical path adjustment attenuation mirror group (32), which is used to change the laser beam diameter and direction and is arranged at intervals with each of the reflection attenuation mirror groups (31) in the optical path.

3. The optical tweezers optical path module according to claim 2, characterized in that, The plurality of said reflection attenuation mirror groups (31) include a first-order reflection attenuation mirror group (311), a second-order reflection attenuation mirror group (312), and a third-order reflection attenuation mirror group (313); The first-stage reflection attenuation mirror group (311), the second-stage reflection attenuation mirror group (312), the optical path adjustment attenuation mirror group (32), and the third-stage reflection attenuation mirror group (313) are arranged sequentially at intervals in the optical path direction, and the first-stage reflection attenuation mirror group (311) is close to the laser head mounting base (20) in the optical path direction.

4. The optical tweezers optical path module according to claim 2, characterized in that, The optical path adjustment attenuation mirror group (32) includes a first aperture (321), a second aperture (322), and a beam expander unit (323); The first aperture (321) and the second aperture (322) are located upstream and downstream of the beam expander unit (323) respectively in the optical path direction.

5. The optical tweezers optical path module according to claim 4, characterized in that, The beam expander unit (323) includes a beam expander (3231), a front upright plate (3232), a rear upright plate (3233), a movable block (3234), a first adjusting rod group (3235), and a second adjusting rod group (3236); The front upright plate (3232) and the rear upright plate (3233) are arranged at intervals in the optical path and are connected by the first adjusting rod group (3235); The rear upright plate (3233) is provided with a central through hole, the movable block (3234) is disposed in the central through hole and is connected to the movable block (3234) through the second adjusting rod group (3236), and the beam expander (3231) is connected to the movable block (3234); The first adjusting rod group (3235) is used to cause the rear upright plate (3233) to drive the front upright plate (3232) and the beam expander (3231) to swing relative to the front upright plate (3232). The second adjusting rod group (3236) is used to cause the movable block (3234) to drive the beam expander (3231) to move.

6. The optical tweezers optical path module according to claim 3, characterized in that, The multi-stage attenuation module (30) also includes a reflector (33), which is located in the optical path and between the optical path adjustment attenuation mirror group (32) and the three-stage reflection attenuation mirror group (313).

7. The optical tweezers optical path module according to claim 6, characterized in that, It also includes a shutter (40) disposed on the support base (10), the shutter (40) being located downstream of the third-order reflection attenuation mirror group (313) in the optical path direction; The shutter (40), the laser head mounting base (20), and the first-stage reflective attenuation mirror group (311) are arranged sequentially at intervals along the length of the support base (10) to form a first column; The reflector (33), the optical path adjustment attenuation mirror group (32), and the secondary reflection attenuation mirror group (312) are arranged sequentially at intervals along the length of the support base (10) to form a second column; The first column and the second column are arranged at intervals in the width direction of the support base (10), and the three-stage reflective attenuation mirror group (313) is located above the reflector (33) and aligned with the shutter (40) in the height direction of the support base (10).

8. The optical tweezers optical path module according to claim 7, characterized in that, The support base (10) includes a support base plate (11), a first mounting plate (12), a second mounting plate (13), a reflector frame (14), an output mirror stand (15), and a shutter stand (16); The first mounting plate (12), the second mounting plate (13), the reflector frame (14), the output mirror stand (15), and the shutter stand (16) are all detachably connected to the support base plate (11). The first mounting plate (12) and the reflector frame (14) are configured to be able to adjust their installation positions in the length direction of the support base (10), and the second mounting plate (13) and the output mirror stand (15) are configured to be able to adjust their installation positions in the width direction of the support base (10). The first-stage reflective attenuation mirror assembly (311) is detachably connected to the first mounting plate (12) and is configured to be able to adjust its relative angle position with respect to the first mounting plate (12); The secondary reflection attenuation mirror assembly (312) is detachably connected to the second mounting plate (13) and is configured to be able to adjust the relative angle with the second mounting plate (13); The three-stage reflection attenuation mirror assembly (313) is detachably connected to the output mirror stand plate (15) and is configured to adjust the installation position in the height direction of the support base (10) and the relative angle with the output mirror stand plate (15). The shutter stand (16) is provided with a shutter mounting bracket (161), which is used to mount the shutter (40) and is configured to be able to adjust the mounting position in the length direction of the support base (10).

9. The optical tweezers optical path module according to any one of claims 1 to 8, characterized in that, The reflection attenuation mirror assembly (31) includes a lens (3101), an opening plate (3102), a mating plate (3103), and multiple adjusting rods (3104); The lens (3101) is disposed on the opening plate (3102). The opening plate (3102) and the mating plate (3103) are arranged at intervals and connected by a plurality of the adjusting rods (3104). The plurality of adjusting rods (3104) can act on the opening plate (3102) to allow the opening plate (3102) to drive the lens (3101) to swing relative to the mating plate (3103).

10. A cell selection system, characterized in that, It includes an optical microscope and an optical tweezers optical path module (100) according to any one of claims 1 to 9, wherein the optical microscope is located downstream of the optical tweezers optical path module (100) in the optical path direction.

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