Perfusion device and biological sample microstructure observation system

Abstract

The utility model provides a perfusion device and biological sample microstructure observation system, including device main part, device main part is provided with liquid inlet channel, liquid outlet channel and sample storehouse, sample storehouse only bottom is open, liquid inlet channel communicates device main part outside with sample storehouse, liquid outlet channel communicates device main part outside with sample storehouse. The position, corresponding to the sample bin, of the bottom of the device body is used for being connected with the cover glass, the top edge of the cover glass is detachably connected with the bottom face of the device body, and after connection, the cover glass completely seals the sample bin. According to the perfusion device, the liquid medicine of the preset type or the preset concentration can be conveyed into the sample bin through the liquid inlet channel, then the effect of rapidly replacing the type of the liquid medicine or accurately regulating and controlling the concentration of the liquid medicine is achieved, when the biological samples need to be replaced, only the cover glass loaded with different biological samples needs to be replaced, and operation is convenient. The in-situ observation effect of the biological sample can be realized.

Description

Technical Field

[0001] This utility model relates to the field of biological and medical equipment technology, and in particular to a perfusion device and a biological sample microstructure observation system. Background Technology

[0002] Confocal laser scanning microscopy (CLSM), as a core tool of modern microscopic imaging technology, has been widely applied in cell biology, botany, and other fields due to its unique optical sectioning capabilities, submicron-level spatial resolution, and three-dimensional dynamic imaging functions. It provides crucial technical support for the real-time observation of dynamic processes in living cells. Through the labeling and excitation of specific fluorescent probes (such as the calcium ion probe Fluo-4 and fluorescent protein-labeled receptors), researchers can directly analyze the dynamic response mechanisms of plant cells to external stimuli, such as transient fluctuations in ion concentration, subcellular protein localization and migration, and organelle interaction network reconstruction, thereby revealing the molecular dynamics of plant physiological activities.

[0003] However, in the practical application of CLSM, traditional open sample observation modes such as conventional slides or petri dishes have the following problems:

[0004] First, the lack of rapid switching and precise concentration control methods for the application of external stimuli such as drugs or ionic solutions leads to a significant deviation between the signal acquisition time window and the drug action time axis. The start and decay phases of the dynamic response process are difficult to capture accurately, affecting the reliability of kinetic parameter analysis.

[0005] Secondly, traditional sample carriers (such as single-well petri dishes) can only support the successive detection of a single or a small number of samples. Multiple parallel experiments require frequent sample replacement, which is not only time-consuming but also makes it difficult to ensure the consistency of environmental conditions (such as temperature and gas concentration), significantly reducing experimental efficiency and data comparability. Utility Model Content

[0006] This invention aims to solve at least one of the technical problems existing in the prior art. To this end, this invention provides an irrigation device and a biological sample microstructure observation system, aiming to overcome the technical bottlenecks in rapid replacement of pesticide solutions, pesticide concentration control, and simultaneous detection of multiple samples, providing a reliable technical platform for real-time analysis of the dynamic response of plant living cells.

[0007] This utility model provides a perfusion device, including a main body, which has an inlet channel, an outlet channel, and a sample chamber. The sample chamber is open only at the bottom. One end of the inlet channel is connected to the outside of the main body, and the other end is connected to the sample chamber. One end of the outlet channel is connected to the outside of the main body, and the other end is connected to the sample chamber. The bottom of the main body, corresponding to the sample chamber, is used for detachable connection with a cover glass. When the cover glass is connected to the main body, it is used to close the sample chamber.

[0008] According to the irrigation device provided by this utility model, a first liquid storage chamber is also provided inside the main body of the device. The first liquid storage chamber is located on the path of the liquid inlet channel, and the position where the liquid inlet channel communicates with the first liquid storage chamber is higher than the bottom of the first liquid storage chamber.

[0009] According to the irrigation device provided by this utility model, a second liquid storage chamber is also provided inside the main body of the device. The second liquid storage chamber is located on the path of the liquid outlet channel, and the position where the liquid outlet channel communicates with the second liquid storage chamber is higher than the bottom of the second liquid storage chamber.

[0010] According to the perfusion device provided by this utility model, the positions where the liquid inlet channel and the liquid outlet channel communicate with the sample chamber are both higher than the bottom of the sample chamber.

[0011] According to the irrigation device provided by this utility model, the inner diameter of the liquid inlet channel and the liquid outlet channel is 1.5 mm to 2.5 mm.

[0012] According to the irrigation device provided by this utility model, the inner diameter of both the inlet channel and the outlet channel is 2 mm.

[0013] According to the irrigation device provided by this utility model, the liquid inlet channel is connected to the outside of the device body through a first insertion hole. A first adapter is inserted into the end of the first insertion hole away from the liquid inlet channel. The extended end of the first adapter is used to connect with the drug supply pipe. The first adapter is interference-fitted with the first insertion hole and the drug supply pipe.

[0014] According to the irrigation device provided by this utility model, the liquid inlet channel is connected to the outside of the device body through a second insertion hole. A second adapter is inserted into the end of the second insertion hole away from the liquid outlet channel. The extended end of the second adapter is used to connect with the liquid recovery tube. The second adapter is press-fitted with the second insertion hole and the liquid recovery tube.

[0015] This invention also provides a system for observing the microstructure of biological samples, including a laser scanning confocal microscope and a perfusion device as described above, wherein the perfusion device is used to be positioned at the observation location of the laser scanning confocal microscope.

[0016] This utility model has the following advantages due to the adoption of the above technical solution:

[0017] The perfusion device provided by this utility model includes a main body with an inlet channel, an outlet channel, and a sample chamber. The sample chamber is open only at the bottom. One end of the inlet channel communicates with the outside of the main body and the other end communicates with the sample chamber, for delivering a predetermined type or concentration of drug solution into the sample chamber. One end of the outlet channel communicates with the outside of the main body and the other end communicates with the sample chamber, for delivering the drug solution from the sample chamber to the outside of the main body. A cover glass is attached to the bottom of the main body corresponding to the sample chamber. The cover glass, located on one side inside the sample chamber, holds the biological sample to be observed. The top edge of the cover glass is detachably connected to the bottom surface of the main body, completely sealing the sample chamber after connection. This perfusion device can deliver a predetermined type or concentration of drug solution into the sample chamber through the inlet channel, enabling rapid replacement of the drug solution type or precise control of the drug solution concentration. Furthermore, when it is necessary to change the biological sample, only the cover glass containing the different biological sample needs to be replaced, achieving in-situ observation of the biological sample.

[0018] Furthermore, the biological sample microstructure observation system provided by this utility model has the same advantages as described above because it is equipped with the perfusion device as described above. Attached Figure Description

[0019] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0020] Figure 1 This is a longitudinal cross-sectional structural diagram of an irrigation device provided in an embodiment of this utility model;

[0021] Figure 2 This is a cross-sectional structural diagram of an irrigation device provided in an embodiment of the present invention;

[0022] Figure 3 This is a top view of an irrigation device provided in an embodiment of the present invention;

[0023] Figure 4This is a bottom view of an irrigation device provided in an embodiment of this utility model.

[0024] Figure label:

[0025] 100: Main body of the device; 200: Liquid inlet channel; 300: Liquid outlet channel; 400: Sample chamber; 500: First liquid storage chamber; 600: Second liquid storage chamber; 700: First insertion port; 800: Second insertion port; 900: Cover glass slide; 1000: First adapter; 1100: Drug supply tube; 1200: Second adapter; 1300: Drug recovery tube. Detailed Implementation

[0026] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0027] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0028] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0029] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0030] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0031] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0032] The perfusion device provided by this utility model includes a main body with an inlet channel, an outlet channel, and a sample chamber. The sample chamber is open only at the bottom. One end of the inlet channel communicates with the outside of the main body and the other end communicates with the sample chamber, for delivering a predetermined type or concentration of drug solution into the sample chamber. One end of the outlet channel communicates with the outside of the main body and the other end communicates with the sample chamber, for delivering the drug solution from the sample chamber to the outside of the main body. A cover glass is attached to the bottom of the main body corresponding to the sample chamber. The cover glass, located on one side inside the sample chamber, holds the biological sample to be observed. The top edge of the cover glass is detachably connected to the bottom surface of the main body, completely sealing the sample chamber after connection. This perfusion device can deliver a predetermined type or concentration of drug solution into the sample chamber through the inlet channel, enabling rapid replacement of the drug solution type or precise control of the drug solution concentration. Furthermore, when it is necessary to change the biological sample, only the cover glass containing the different biological sample needs to be replaced, achieving in-situ observation of the biological sample.

[0033] The following is combined Figures 1 to 4 This invention describes the irrigation device.

[0034] This utility model provides a perfusion device, including a device body 100. The device body 100 is provided with an inlet channel 200, an outlet channel 300, and a sample chamber 400. The sample chamber 400 is open only at the bottom. One end of the inlet channel 200 is connected to the outside of the device body 100, and the other end of the inlet channel 200 is connected to the sample chamber 400. One end of the outlet channel 300 is connected to the outside of the device body 100, and the other end of the inlet channel 200 is connected to the sample chamber 400. The bottom of the device body 100, corresponding to the position of the sample chamber 400, is used for detachable connection with a cover glass 900. When the cover glass 900 is connected to the device body 100, the cover glass 900 is used to close the sample chamber 400.

[0035] Specifically, the perfusion device includes a main body 100, which can be a solid cuboid structure. A sample chamber 400 can be positioned at the center of the main body 100, extending upwards from the bottom of the main body 100 but not penetrating its top, forming a cavity that is closed at the top and open at the bottom. The cross-section of the sample chamber 400 can be square or rectangular, and the bottom opening of the sample chamber 400 can be completely covered by a coverslip 900.

[0036] The liquid inlet channel 200 can be located on the left side of the sample chamber 400, including a horizontal section extending to the left and a vertical section extending upward. The bottom end of the vertical section is connected to the left end of the horizontal section, and the top end of the vertical section is connected to the outer top of the device body 100, where it is connected to the drug supply pipe 1100. The right end of the horizontal section is connected to the sample chamber 400. In this way, the liquid inlet channel 200 can guide the drug supplied by the drug supply pipe 1100 into the sample chamber 400.

[0037] The liquid outlet channel 300 can be located on the right side of the sample chamber 400, including a horizontal section extending to the right and a vertical section extending upward. The bottom end of the vertical section is connected to the right end of the horizontal section, and the top end of the vertical section is connected to the top outer side of the device body 100. This end is used to connect to the drug recovery pipe 1300. The left end of the horizontal section is connected to the sample chamber 400. In this way, the liquid outlet channel 300 can transport the drug solution in the sample chamber 400 to the drug recovery pipe 1300.

[0038] In use, connect the drug supply tube 1100 to the inlet channel 200, connect the drug recovery tube 1300 to the outlet channel 300, and connect the coverslip 900 containing the biological sample to the bottom of the sample chamber 400. At this time, the outlet channel 300, the sample chamber 400 and the outlet channel 300 form a closed structure.

[0039] When it is necessary to change the drug solution or adjust the drug solution concentration, the required type of drug solution or the required concentration of drug solution can be delivered to the sample chamber 400 through the drug solution supply pipe 1100. Excess drug solution is discharged into the drug solution recovery pipe 1300 through the liquid outlet channel 300, so as to realize the rapid change of drug type and the precise adjustment of drug solution concentration.

[0040] When it is necessary to replace the biological sample, simply remove the coverslip 900 and reinstall the coverslip 900 containing the biological sample to be observed, connecting it to the bottom of the sample chamber 400 to achieve the effect of in-situ observation of the biological sample.

[0041] In some embodiments, a first liquid storage tank 500 is further provided in the main body 100 of the device. The first liquid storage tank 500 is located on the path of the liquid inlet channel 200, and the position where the liquid inlet channel 200 communicates with the first liquid storage tank 500 is higher than the bottom of the first liquid storage tank 500.

[0042] Specifically, the first liquid storage chamber 500 can be a cylindrical cavity. The first liquid storage chamber 500 can be connected to the horizontal section of the liquid inlet channel 200. Before the liquid enters the sample chamber 400, the liquid first enters the first liquid storage chamber 500. After filling the space below the connection between the first liquid storage chamber 500 and the liquid inlet channel 200, the liquid overflows upward into the latter half of the liquid inlet channel 200, thus making the flow more stable.

[0043] A second liquid storage chamber 600 can also be provided inside the main body 100 of the device. The second liquid storage chamber 600 is located on the path of the liquid outlet channel 300. The position where the liquid outlet channel 300 connects with the second liquid storage chamber 600 is also higher than the bottom of the second liquid storage chamber 600. A portion of the drug solution can be stored in the second liquid storage chamber 600 to prevent the drug solution from flowing back into the sample chamber 400.

[0044] In some embodiments, the positions where the inlet channel 200 and the outlet channel 300 are connected to the sample chamber 400 are both higher than the bottom of the sample chamber 400. Thus, the sample chamber 400 forms a groove below the positions where it is connected to the inlet channel 200 and the outlet channel 300, which can store a certain amount of drug solution, thereby allowing the biological sample to be placed in the drug solution.

[0045] In some embodiments, the inner diameter of the liquid inlet channel 200 and the liquid outlet channel 300 can be 1.5 mm to 2.5 mm, specifically 2 mm.

[0046] In some embodiments, the top of the device body 100 is closed, and a first insertion hole 700 is provided at the top of the device body 100 at a position corresponding to the vertical section of the liquid inlet channel 200. The inner diameter of the first insertion hole 700 is smaller than the inner diameter of the liquid inlet channel 200. The top end of the first insertion hole 700 is used to insert a first adapter 1000. The first adapter 1000 and the first insertion hole 700 are interference-fitted to ensure a sealed contact. The top end of the first adapter 1000 is used to insert into a medicine supply pipe 1100. The first adapter 1000 and the medicine supply pipe 1100 are also interference-fitted to ensure a sealed contact.

[0047] Specifically, the first insertion hole 700 vertically connects the exterior of the device body 100 to the liquid inlet channel 200. The inner diameter of the first insertion hole can be 1.6 mm. The first adapter 1000 can be a second adapter pipe with an outer diameter of 1.8 mm. The outer diameter of the second adapter pipe is slightly larger than the inner diameter of the first insertion hole 700 to achieve an interference fit. The inner diameter of the liquid supply pipe 1100 can be 1.6 mm, and the outer diameter of the second adapter pipe is slightly larger than the inner diameter of the liquid supply pipe 1100 to achieve an interference fit.

[0048] Similarly, a second insertion hole 800 is provided at the top of the device body 100, corresponding to the vertical section of the liquid outlet channel 300. The inner diameter of the second insertion hole 800 is smaller than the inner diameter of the liquid outlet channel 300. The top end of the second insertion hole 800 is used to insert a second adapter 1200. The second adapter 1200 and the second insertion hole 800 are interference-fitted to ensure a sealed contact. The top end of the second adapter 1200 is used to insert into the liquid recovery pipe 1300. The second adapter 1200 and the liquid recovery pipe 1300 are also interference-fitted to ensure a sealed contact.

[0049] Specifically, the second insertion hole 800 vertically connects the exterior of the device body 100 to the liquid outlet channel 300. The inner diameter of the first insertion hole can be 1.6 mm. The second adapter 1200 can be a second adapter tube with an outer diameter of 1.8 mm. The outer diameter of the second adapter tube is slightly larger than the inner diameter of the second insertion hole 800 to achieve an interference fit. The inner diameter of the liquid recovery tube 1300 can be 1.6 mm, and the outer diameter of the second adapter tube is slightly larger than the inner diameter of the liquid recovery tube 1300 to achieve an interference fit.

[0050] An embodiment of this invention also provides a system for observing the microstructure of biological samples, including a laser scanning confocal microscope and a perfusion device as described above, wherein the perfusion device is disposed at the observation position of the laser scanning confocal microscope. Because it incorporates the perfusion device described above, it possesses the same advantages as described above.

[0051] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and not to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. These modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application, and they should all be covered within the scope of the claims and specification of this application. In particular, as long as there is no structural conflict, the various technical features mentioned in the embodiments can be combined in any way. This application is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. An irrigation device, characterized in that, The device includes a main body (100), which has an inlet channel (200), an outlet channel (300), and a sample chamber (400). The sample chamber (400) is open only at the bottom. One end of the inlet channel (200) is connected to the outside of the main body (100), and the other end of the inlet channel (200) is connected to the sample chamber (400). One end of the outlet channel (300) is connected to the outside of the main body (100), and the other end of the inlet channel (200) is connected to the sample chamber (400). The bottom of the main body (100) is positioned corresponding to the sample chamber (400) for detachable connection with a cover glass (900). When the cover glass (900) is connected to the main body (100), it is used to close the sample chamber (400).

2. The irrigation device according to claim 1, characterized in that, The main body (100) of the device is also provided with a first liquid storage tank (500), which is located on the path of the liquid inlet channel (200). The position where the liquid inlet channel (200) communicates with the first liquid storage tank (500) is higher than the bottom of the first liquid storage tank (500).

3. The irrigation device according to claim 2, characterized in that, The main body (100) of the device is also provided with a second liquid storage tank (600), which is located on the path of the liquid outlet channel (300). The position where the liquid outlet channel (300) communicates with the second liquid storage tank (600) is higher than the bottom of the second liquid storage tank (600).

4. The irrigation device according to claim 1, characterized in that, The liquid inlet channel (200) and the liquid outlet channel (300) are both located above the bottom of the sample chamber (400) when they connect to the sample chamber (400).

5. The irrigation device according to claim 1, characterized in that, The inner diameter of the liquid inlet channel (200) and the liquid outlet channel (300) is 1.5 mm to 2.5 mm.

6. The irrigation device according to claim 5, characterized in that, The inner diameter of both the liquid inlet channel (200) and the liquid outlet channel (300) is 2 mm.

7. The irrigation device according to claim 1, characterized in that, The liquid inlet channel (200) and the device body (100) are connected to the outside of the device body (100) through the first socket (700). A first adapter (1000) is inserted into the end of the first socket (700) away from the liquid inlet channel (200). The extended end of the first adapter (1000) is used to connect with the liquid supply pipe (1100). The first adapter (1000) is press-fitted with the first socket (700) and the liquid supply pipe (1100).

8. The irrigation device according to claim 1, characterized in that, The liquid inlet channel (200) is connected to the outside of the device body (100) through the second socket (800). A second adapter (1200) is inserted into the end of the second socket (800) away from the liquid outlet channel (300). The extended end of the second adapter (1200) is used to connect with the liquid recovery tube (1300). The second adapter (1200) is press-fitted with the second socket (800) and the liquid recovery tube (1300).

9. A system for observing the microstructure of biological samples, characterized in that, The invention includes a laser scanning confocal microscope and an irrigation device as described in any one of claims 1 to 8, wherein the irrigation device is configured to be positioned at the observation location of the laser scanning confocal microscope.

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