Adjustable protective tissue culture inoculation tool

Abstract

This utility model relates to the field of veterinary medical devices, and more particularly to an adjustable protective tissue culture inoculation tool, comprising a U-shaped clamp and a limiting component. A mounting base is fixed to the side wall of the U-shaped clamp. The limiting component includes a threaded post threadedly connected to the middle of the mounting base. A support plate is fixed to the end of the threaded post. A limiting seat is fixed to the inner side wall of the U-shaped clamp near the support plate. Support posts are fixed to both ends of the U-shaped clamp, and protective components are provided at the ends of the support posts. Each protective component includes a rubber sleeve fitted over the outer surface of the support post. Compared to traditional tissue culture inoculation tools, this utility model improves the convenience of adjusting the position of the support plate through the engagement of the mounting base and the threaded post. The engagement of the limiting seat and the support plate improves the stability of the support post end support and limiting. The engagement of the rubber sleeve, the first slot, and the second slot further improves the adaptability of the clamping, thereby avoiding clamping damage to the explant and improving the quality of tissue culture inoculation.

Description

Technical Field

[0001] This utility model relates to the field of veterinary medical devices, and in particular to an adjustable protective tissue culture inoculation tool. Background Technology

[0002] Plant tissue culture, also known as plant tissue culture, is a technique that, under sterile conditions, cultivates explants such as isolated plant organs, tissues, cells, or protoplasts on artificially prepared culture media, providing suitable culture conditions to allow them to grow, differentiate, and form complete plants. During tissue culture inoculation, operators typically use forceps as inoculation tools to inoculate the cut explants onto the culture medium, ensuring that the cut of the explant is in full contact with the culture medium solution to facilitate nutrient absorption and growth.

[0003] Existing tissue culture inoculation tools typically use tweezers for gripping. Tweezers are usually made of bent metal with sharp tips. Since explants are delicate, differences in the gripping force applied by operators when using tweezers may cause damage to the explants, thus affecting the quality of tissue culture inoculation. Utility Model Content

[0004] To overcome the problem that existing tissue culture inoculation tools typically use tweezers for gripping, tweezers are usually made of bent metal with sharp tips. Since explants are relatively delicate, differences in the force applied by operators when using tweezers may cause damage to the explants, thus affecting the quality of tissue culture inoculation.

[0005] The technical solution of this utility model is as follows: an adjustable protective tissue culture inoculation tool, including a U-shaped clamp and a limiting component. A mounting seat is fixed on the side wall of the U-shaped clamp. The limiting component includes a threaded post threadedly connected to the middle of the mounting seat. A support plate is fixed at the end of the threaded post. A limiting seat is fixed on the inner side wall of the U-shaped clamp near the support plate. Support posts are fixed at both ends of the U-shaped clamp. A protective component is provided at the end of each support post. Each protective component includes a rubber sleeve fitted on the outer surface of the support post. A first slot is opened on the side wall of each rubber sleeve. A second slot is opened on the side wall of each rubber sleeve near the first slot.

[0006] Furthermore, a threaded groove is provided in the middle of the mounting base, and the threaded column is threadedly connected in the middle of the threaded groove. The internal dimensions of the threaded groove are adapted to the external dimensions of the threaded column, thereby improving the stability of the rotation of the threaded column.

[0007] Furthermore, a butterfly-shaped knob is fixed at the end of the threaded column away from the support plate, and both the support plate and the limiting seat are circular, which improves the stability of the threaded column support.

[0008] Furthermore, the first and second card slots are symmetrically distributed.

[0009] Furthermore, each of the rubber sleeves has a first slot in the middle, and the support posts are inserted into the first slot in sequence.

[0010] Furthermore, several third slots are provided on the inner sidewall of the first slot, and several protrusions are fixed on the sidewall of the support column. The external dimensions of the support column and the protrusions are adapted to the internal dimensions of the first slot and the third slot, respectively, which improves the stability of the rubber sleeve installation.

[0011] Furthermore, the outer surface of the U-shaped clamp is fitted with two protective sleeves, which are symmetrically distributed.

[0012] Furthermore, anti-slip grooves are provided on the side walls of the protective sleeve, and a second slot is provided in the middle of the protective sleeve for the U-shaped clip to be inserted, which improves the stability of the U-shaped clip grip.

[0013] The beneficial effects of this utility model are:

[0014] Compared to traditional tissue culture inoculation tools, the installation base and threaded column improve the convenience of adjusting the position of the support plate. The limiting seat and support plate improve the stability of the support column end support and limiting. The rubber sleeve, first slot and second slot further improve the clamping adaptability, thereby avoiding clamping damage to the explant and improving the quality of tissue culture inoculation. Secondly, by setting up a protrusion, a first slot and a third slot, the protrusion is sequentially engaged in the third slot, thereby improving the stability of the rubber sleeve. Attached Figure Description

[0015] Figure 1 The diagram shown is a schematic representation of the overall structure of the tissue culture inoculation tool of this utility model.

[0016] Figure 2 The diagram shown is a schematic representation of the U-shaped clip structure of this utility model.

[0017] Figure 3 The diagram shown is an exploded structural illustration of this utility model.

[0018] Figure 4 The diagram shown is a schematic representation of the support column structure of this utility model.

[0019] Figure 5 The diagram shown is a schematic representation of the protective component structure of this utility model.

[0020] Explanation of reference numerals in the attached drawings: 1. U-shaped clip; 2. Mounting base; 3. Limiting component; 301. Threaded post; 302. Support plate; 303. Butterfly knob; 4. Support post; 5. Protective component; 501. Rubber sleeve; 502. First slot; 503. Second slot; 504. First slot; 505. Third slot; 6. Limiting seat; 7. Protective sleeve; 8. Threaded groove; 9. Protrusion; 10. Anti-slip groove; 11. Second slot. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0022] Among the currently discovered feasible technologies, the following are described:

[0023] Plants, as one of the most important life forms on Earth, are not only the cornerstone of ecosystems but also provide humans with vital resources such as food, medicine, and fiber. In today's era of rapid technological advancement, plant tissue culture technology, with its unique advantages, has become a shining jewel in the fields of botanical research and agricultural production. Plant tissue culture, also known as plant tissue culture, refers to the technique of culturing explants such as isolated plant organs, tissues, cells, or protoplasts on artificially prepared culture media under sterile conditions, providing suitable culture conditions to induce the production of callus tissue, adventitious buds, and adventitious roots, ultimately forming a complete plant.

[0024] The core theory of plant tissue culture is the totipotency of plant cells. In 1902, German botanist Haberland proposed the theory of cell totipotency, believing that each cell of a plant contains all the genetic information of the species and has the potential to develop into a complete plant. Over time, this theory has been confirmed in numerous experiments. After being separated from the parent plant, plant cells can regain their ability to divide and differentiate under the induction of suitable nutrients, plant hormones, and external environmental conditions, and thus develop into a complete plant. This characteristic has laid a solid theoretical foundation for the development of plant tissue culture technology.

[0025] Culture medium: The culture medium is one of the key factors in plant tissue culture. It provides the necessary nutrients for the growth and development of explants. It generally includes inorganic nutrients (such as macroelements like nitrogen, phosphorus, and potassium, and microelements like iron, zinc, and manganese), organic nutrients (such as sucrose, vitamins, and amino acids), plant growth regulators (such as auxins and cytokinins), and solidifying agents such as agar. Light: Light also plays an important role in plant tissue culture. Temperature: A suitable temperature is crucial for successful plant tissue culture. The optimal temperature for most plant tissue cultures is between 20-30℃. Temperatures that are too high or too low will affect the growth and differentiation of explants, and may even lead to the death of the culture. Different plant species have slightly different temperature requirements; for example, some tropical plants may require higher culture temperatures, while some temperate plants adapt to lower temperatures. Humidity: The humidity of the culture environment also needs to be controlled. Because the culture medium contains abundant water and nutrients, it is prone to microbial growth. Therefore, a relatively sterile environment needs to be maintained. Generally, the air humidity in the culture room should be around 70%-80% to prevent the culture medium from drying out and to prevent contamination by other microorganisms.

[0026] Selection and disinfection of explants: Select vigorous plant tissues or organs free from pests and diseases as explants, such as stem tips, leaves, and anthers. Then, perform strict disinfection treatment on the explants. Usually, first soak them in 70%-75% alcohol for a few seconds to a few minutes, then soak them in 0.1%-1% mercuric chloride solution or sodium hypochlorite solution for 10-30 minutes, and finally rinse them with sterile water 3-5 times to remove microorganisms from the surface of the explants.

[0027] Inoculation: On a sterile operating table, cut the sterilized explants into appropriate sizes and inoculate them onto the pre-prepared culture medium. Strict aseptic techniques must be followed during the inoculation process to prevent contamination by other microorganisms. Attention should be paid to the orientation and position of the explants during inoculation to facilitate their growth and differentiation.

[0028] Culture: Place the inoculated culture bottles or dishes into a culture room and culture them according to the set culture conditions. During the culture process, observe the growth of the explants regularly, such as whether there is contamination, callus formation, and differentiation of buds and roots, and record and deal with any abnormalities in a timely manner. Generally, the culture medium needs to be changed every certain period of time (e.g., 1-2 weeks) to replenish nutrients and remove metabolic waste.

[0029] Transplanting: When the test-tube seedlings grow to a certain size and have a complete root, stem, and leaf structure, they need to be transplanted to a natural environment such as a greenhouse or field. Before transplanting, the test-tube seedlings should be hardened off by gradually reducing the humidity in the culture container and increasing the light intensity to allow them to adapt to the external environment. Then, remove the test-tube seedlings from the culture medium, wash the culture medium off the roots, and transplant them into flowerpots or seedbeds filled with suitable substrate (such as vermiculite, perlite, or nutrient soil). Water thoroughly to settle the roots, and pay attention to maintaining suitable temperature, humidity, and light conditions to improve the transplant survival rate.

[0030] When inoculating tissue culture, the first step is to enter the inoculation room: the operator puts on work clothes, hat, mask and gloves, and enters the inoculation room through the buffer room.

[0031] Placement of items: Neatly place the sterilized explants, culture media, inoculation tools, etc. on the surface of the clean bench to avoid unnecessary cross-contamination.

[0032] Light the alcohol lamp: Light the alcohol lamp on one side of the laminar flow hood to create a localized sterile environment. Inoculation tools should be handled near the flame of the alcohol lamp.

[0033] Cutting explants: Remove the explants with scalpels that have been cauterized and cooled, place them on sterile filter paper, and blot off any surface moisture. Depending on the culture purpose, cut the explants into appropriate sizes using scissors or a scalpel; generally, cut stem tips into 0.1-0.5 cm pieces and leaves into 0.5-1 cm pieces. 2 Small pieces.

[0034] Inoculation of explants: Open the cap of the culture bottle, quickly flask the bottle mouth over an alcohol lamp flame to sterilize the bottle mouth, use tweezers to pick up the cut explants, gently place them on the culture medium, arrange them at certain intervals and directions, flask the bottle mouth again, and quickly close the cap.

[0035] Example 1

[0036] Please refer to Figures 1-5 An adjustable protective tissue culture inoculation tool includes a U-shaped clamp 1 and a limiting component 3. The U-shaped clamp 1 is made of stainless steel, and a mounting base 2 is fixedly mounted on the side wall of the U-shaped clamp 1. The limiting component 3 includes a threaded post 301 threadedly connected to the middle of the mounting base 2. A support plate 302 is fixedly mounted at the end of the threaded post 301. A threaded groove 8 is opened in the middle of the mounting base 2, and the threaded post 301 is threadedly connected to the middle of the threaded groove 8. The internal dimensions of the threaded groove 8 are adapted to the external dimensions of the threaded post 301 to improve the rotational stability of the threaded post 301. A limiting seat 6 is fixedly mounted on the inner side wall of the U-shaped clamp 1 near the support plate 302, and a butterfly-shaped spiral is fixedly mounted at the end of the threaded post 301 away from the support plate 302. Button 303 improves the ease of rotation of threaded post 301. Support plate 302 and limit seat 6 are both circular, which improves the stability of threaded post 301 support. Support posts 4 are fixed at both ends of U-shaped clamp 1. Protective components 5 are provided at the ends of support posts 4. Protective components 5 include rubber sleeves 501 sleeved on the outer surface of support posts 4. Rubber sleeves 501 are made of soft and elastic silicone. First slots 502 are provided on the side walls of rubber sleeves 501. Second slots 503 are provided on the side walls of rubber sleeves 501 near the first slots 502. The first slots 502 and the second slots 503 are symmetrically distributed to facilitate clamping of explants of different diameters.

[0037] Each of the rubber sleeves 501 has a first slot 504 in the middle. The support column 4 is inserted into the first slot 504 in sequence. Several third slots 505 are opened on the inner side wall of the first slot 504. Several protrusions 9 are fixed on the side wall of the support column 4. The external dimensions of the support column 4 and the protrusions 9 are adapted to the internal dimensions of the first slot 504 and the third slot 505, respectively, which improves the stability of the rubber sleeve 501 installation.

[0038] Two protective sleeves 7 are fitted on the outer surface of the U-shaped clip 1. The protective sleeves 7 are made of plastic and are symmetrically distributed. Anti-slip grooves 10 are provided on the side walls of the protective sleeves 7 to increase friction resistance. A second slot 11 is provided in the middle of each protective sleeve 7 for the U-shaped clip 1 to be inserted, which improves the gripping stability of the U-shaped clip 1.

[0039] When using this tissue culture inoculation tool, the operator first grasps the end of the U-shaped clamp 1 with their hand. The anti-slip groove 10 is used to increase the contact area, thereby improving the stability of the operator's grip on the U-shaped clamp 1. Then, the two protective sleeves 7 are pressed, which causes the two ends of the U-shaped clamp 1 to rotate inward, thereby driving the support column 4 to move synchronously, so that the rubber sleeve 501 clamps the side wall of the explant. The explant is cut into an appropriate size with scissors or a scalpel according to the culture purpose, generally 0.1-0.5cm from the stem tip. The explant is existing technology and will not be described in detail here. The operator selects the first slot 502 or the second slot 503 according to the size of the explant in turn, and the support plate 302 is supported on the side wall of the limiting seat 6, which restricts the displacement of the support column 4, thereby avoiding excessive clamping by the rubber sleeve 501. The operator can adjust the length of the threaded column 301 threaded connection inside the threaded groove 8 by rotating the butterfly knob 303, thereby further improving the adaptability of the inoculation tool clamping.

[0040] Furthermore, considering the possibility of the rubber sleeve 501 sliding and loosening, the protrusions 9 are sequentially engaged inside the third slot 505, thereby improving the stability of the rubber sleeve 501 on the outer surface of the support column 4.

[0041] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An adjustable containment tool for tissue culture inoculation, characterized in that, It includes a U-shaped clamp (1) and a limiting component (3): a mounting base (2) is fixed on the side wall of the U-shaped clamp (1), and the limiting component (3) includes a threaded post (301) threadedly connected to the middle of the mounting base (2). A support plate (302) is fixed at the end of the threaded post (301). A limiting seat (6) is fixed on the inner side wall of the U-shaped clamp (1) near the support plate (302). Support posts (4) are fixed at both ends of the U-shaped clamp (1). A protective component (5) is provided at the end of each support post (4). Each protective component (5) includes a rubber sleeve (501) sleeved on the outer surface of the support post (4). A first slot (502) is provided on the side wall of each rubber sleeve (501). A second slot (503) is provided on the side wall of each rubber sleeve (501) near the first slot (502).

2. The adjustable guard tool for tissue culture inoculation of claim 1, wherein: The mounting base (2) has a threaded groove (8) in the middle, and the threaded post (301) is threaded in the middle of the threaded groove (8). The internal dimensions of the threaded groove (8) are adapted to the external dimensions of the threaded post (301).

3. The adjustable guard tool for tissue culture inoculation of claim 1, wherein: A butterfly knob (303) is fixed at the end of the threaded column (301) away from the support plate (302). Both the support plate (302) and the limiting seat (6) are circular.

4. The adjustable guard tool for tissue culture inoculation of claim 1, wherein: The first card slot (502) and the second card slot (503) are symmetrically distributed.

5. The adjustable guard tool for inoculating tissue culture according to claim 1, wherein: Each sleeve (501) has a first slot (504) in the middle, and the support column (4) is inserted into the first slot (504) in sequence.

6. The adjustable guard tool for tissue culture inoculation of claim 5, wherein: The inner wall of the first slot (504) is provided with several third slots (505), and several protrusions (9) are fixed on the side wall of the support column (4). The external dimensions of the support column (4) and the protrusions (9) are respectively adapted to the internal dimensions of the first slot (504) and the third slots (505).

7. The adjustable guard tool for inoculating tissue culture according to claim 1, wherein: The outer surface of the U-shaped clip (1) is fitted with two protective sleeves (7), which are symmetrically distributed.

8. The adjustable guard tool for tissue culture inoculation of claim 7, wherein: Anti-slip grooves (10) are provided on the side walls of the protective sleeve (7), and a second slot (11) is provided in the middle of the protective sleeve (7) for the U-shaped clip (1) to be inserted.

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