A multifunctional reaction column for solid-phase synthesis of phosphorylated cyclic peptides

Abstract

This invention discloses a multifunctional reaction column for solid-phase synthesis of phosphorylated cyclic peptides, comprising a solid-phase extraction column. The solid-phase extraction column is positioned by a support device, which includes a screw with a threaded sleeve threaded onto the screw. Several stabilizing components are evenly distributed on the outer side of the threaded sleeve. The solid-phase extraction column has a top plate at the top, and a protrusion is fixed at the bottom of the top plate. The protrusion is installed in conjunction with the stabilizing components. The threaded sleeve allows free rotation on the screw to adjust the height, i.e., the placement height of the solid-phase extraction column. The top plate supports the surface of the sleeve to prevent the solid-phase extraction column from falling off the sleeve surface. The cooperation between the protrusion and the slot enhances the stability of the solid-phase extraction column, making it less prone to shaking on the sleeve surface. This prevents the solid-phase extraction column from collapsing during liquid addition. The multiple equidistantly distributed stabilizing components can simultaneously place multiple sets of solid-phase extraction columns for synchronous extraction operations.

Description

Technical Field

[0001] This invention belongs to the field of solid-phase extraction column technology, specifically relating to a multifunctional reaction column for solid-phase synthesis of phosphorylated cyclic peptides. Background Technology

[0002] Solid-phase reaction columns, also known as solid-phase extraction columns, refer to the process by which solids react with each other to form new solid products. In a broader sense, any chemical reaction involving a solid phase can be called a solid-phase reaction, including the thermal decomposition of solids, the oxidation of metals in air, and chemical reactions between solids, between solids and liquids, and between solids and gases.

[0003] Existing solid-phase extraction (SPE) columns have some limitations. In solid-phase synthesis apparatus, the placement and stability of the SPE column have a significant impact on the synthesis process and extraction efficiency. Traditional SPE column placement methods often lack flexibility and stability, making it difficult to precisely adjust the column height according to actual synthesis needs. This can lead to collisions, shaking, or even collapse during operations such as liquid addition and reaction, affecting the smooth progress of the synthesis and the quality of the product. Utility Model Content

[0004] The purpose of this invention is to provide a multifunctional reaction column for solid-phase synthesis of phosphorylated cyclic peptides, so as to solve the problem of stable placement of solid-phase extraction columns in the prior art.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A multifunctional reaction column for solid-phase synthesis of phosphorylated cyclic peptides includes a solid-phase extraction column. The solid-phase extraction column is positioned by a support device, which includes a screw with a threaded sleeve threaded onto it. Several stabilizing components are evenly distributed on the outer side of the threaded sleeve. The solid-phase extraction column has a top plate at the top, and a protrusion is fixed at the bottom of the top plate. The protrusion is installed in conjunction with the stabilizing components.

[0007] Preferably, the stabilizing component includes an extension rod, which is fixed to the outer wall of the threaded sleeve. A support arm is fixed to one end of the extension rod, and a sleeve is fixed to one side of the support arm. The surface of the sleeve has a groove that mates with a protrusion, and the inner side of the sleeve has an arc-shaped groove.

[0008] Preferably, the sleeve is extended with extension arms at both ends. The extension arms extend tangentially to the arcuate groove, which does not affect the storage of the solid phase extraction column. At the same time, the extended arms can increase the support area with the top plate, thereby further improving the stability of the solid phase extraction column.

[0009] Preferably, the protrusions are provided in groups of three, symmetrically distributed around the central axis. Using protrusions arranged in groups of three allows for support in two orientations, eliminating the need to deliberately find a suitable orientation for the solid-phase extraction column. Stable placement of the solid-phase extraction column can be achieved whether it is placed from the left or right side, thanks to the mutual cooperation between the protrusions and the slots.

[0010] Preferably, the solid-phase extraction column has a collection tube at the bottom, and the extension rod and the collection tube are fitted with collars, with a corner rod fixed between the two collars.

[0011] Preferably, the collar on the extension rod is slidably connected. The slidably connected collar allows adjustment of the position of the collar at the collection tube, thereby facilitating the assembly of the solid-phase extraction column.

[0012] Preferably, a base is fixed to the bottom of the screw. The base serves as a counterweight, thereby improving the stability of the overall device.

[0013] The technical solution of this utility model has the following beneficial effects:

[0014] 1. The use of threaded sleeves allows free rotation on the screw to adjust the height, i.e., the placement height of the solid phase extraction column. The top plate supports the surface of the sleeve to prevent the solid phase extraction column from falling off the sleeve surface. The cooperation of the protrusion and the slot enhances the stability of the solid phase extraction column, making it less likely to shake on the surface of the sleeve. This prevents the solid phase extraction column from collapsing during liquid addition. Multiple equidistantly distributed stabilizing components can simultaneously place multiple sets of solid phase extraction columns for synchronous extraction operations.

[0015] 2. Inserting the collection tube of the solid phase extraction column into the collar can further improve the stability of the solid phase extraction column. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below.

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0018] Figure 2 This is a partial structural schematic diagram of the present invention.

[0019] Figure 3 This is a schematic diagram of the stabilizing component of this utility model.

[0020] Figure 4 This is a schematic diagram showing the disassembled stable component of this utility model.

[0021] Figure 5 This is a plan view of the stabilizing component assembly of this utility model.

[0022] Figure 6 This is a schematic diagram of the bump distribution structure of this utility model.

[0023] Reference numerals: 10, base; 11, screw; 20, threaded sleeve; 201, extension rod; 202, support arm; 203, sleeve; 204, arc groove; 205, extension arm; 206, slot; 30, solid phase extraction column; 301, protrusion; 302, collection tube; 303, top plate; 40, corner rod; 401, collar. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without inventive effort are within the scope of protection of this utility model.

[0025] Example 1:

[0026] refer to Figure 1 A multifunctional reaction column for solid-phase synthesis of phosphorylated cyclic peptides includes a solid-phase extraction column 30, which is positioned by a support device including a screw 11.

[0027] The bottom of the screw 11 is fixed with a base 10; when the solid phase extraction column 30 is placed on the support device for extraction, the base 10 is used as a counterweight to improve the stability of the overall device.

[0028] refer to Figures 1-4 The screw 11 is threaded with a threaded sleeve 20, and several stabilizing components are evenly distributed on the outer side of the threaded sleeve 20. The use of the threaded sleeve 20 allows it to rotate freely on the screw 11 to adjust its height, i.e., the placement height of the solid phase extraction column 30.

[0029] The solid phase extraction column 30 has a top plate 303 at the top, and a protrusion 301 is fixed at the bottom of the top plate 303. The protrusion 301 is installed in conjunction with the stabilizing component.

[0030] The stabilizing component includes an extension rod 201, which is fixed to the outer wall of the threaded sleeve 20. One end of the extension rod 201 is fixed with a support arm 202, and a sleeve 203 is fixed to one side of the support arm 202. The surface of the sleeve 203 is provided with a slot 206 that mates with the protrusion 301, and the inner side of the sleeve 203 is provided with an arc groove 204.

[0031] In the above scheme, when using the solid-phase extraction column 30 for extraction, the solid-phase extraction column 30 is placed on the sleeve plate 203. The arc groove 204 is half the diameter of the solid-phase extraction column 30. The top plate 303 supports the surface of the sleeve plate 203 through the arc groove 204 to prevent the solid-phase extraction column 30 from falling off the surface of the sleeve plate 203. The cooperation between the protrusion 301 and the slot 206 enhances the stability of the solid-phase extraction column 30, making it less likely to shake on the surface of the sleeve plate 203. This prevents the solid-phase extraction column 30 from collapsing when it is hit during liquid addition. Multiple equidistantly distributed stabilizing components can simultaneously place multiple sets of solid-phase extraction columns 30 to perform extraction operations synchronously.

[0032] Preferred Solution Reference Figure 3 and Figure 5 The sleeve plate 203 has extension arms 205 at both ends. The extension arms 205 extend tangentially to the arc groove 204, so as not to affect the storage of the solid phase extraction column 30. At the same time, the extended extension arms 205 can increase the support area with the top plate 303, thereby further improving the stability of the solid phase extraction column 30.

[0033] Further reference Figure 6 The protrusion 301 has three protrusions symmetrically distributed around the central axis;

[0034] The protrusions 301, arranged in groups of three, can support the solid phase extraction column 30 in two directions. There is no need to deliberately find a suitable placement direction for the solid phase extraction column 30. Whether placed from the left or right, the solid phase extraction column 30 can be stably placed, that is, the mutual cooperation between the protrusions 301 and the slots 206.

[0035] Example 2:

[0036] refer to Figure 1 and Figure 2 The bottom of the solid phase extraction column 30 has a collection tube 302, and the extension rod 201 and the collection tube 302 are fitted with collars 401. A corner rod 40 is fixed between the two collars 401.

[0037] In the above scheme, the liquid extracted by the solid phase extraction column 30 is discharged from the bottom of the collection tube 302. The collection tube 302 of the solid phase extraction column 30 is inserted into the collar 401, which can further improve the stability of the solid phase extraction column 30.

[0038] In the preferred embodiment, the collar 401 on the extension rod 201 is slidably connected;

[0039] The sliding collar 401 can adjust the position of the collar 401 at the collection tube 302, thereby facilitating the assembly of the solid phase extraction column 30; or supporting solid phase extraction columns 30 of different sizes (it should be noted here that the size of the top plate 303 and the protrusion 301 of the solid phase extraction column 30 remains unchanged, and changes in the size of the solid phase extraction column 30 do not affect the fit with the sleeve plate 203).

[0040] The specific implementation process of this utility model is as follows:

[0041] When the solid phase extraction column 30 is performing extraction, it is placed on the sleeve plate 203, and the top plate 303 is supported on the surface of the sleeve plate 203. The stability of the solid phase extraction column 30 is enhanced by the cooperation of the protrusion 301 and the slot 206. The collection tube 302 of the solid phase extraction column 30 is inserted into the collar 401, which can further improve the stability of the solid phase extraction column 30.

[0042] The above embodiments are merely exemplary models of this utility model and are not intended to limit this utility model. The scope of protection of this utility model is defined by the claims. Various modifications or equivalent substitutions can be made to this utility model within its substance and scope of protection. Such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this utility model.

[0043] In the description of this utility model, it should be noted that the terms "inner," "front," "rear," "left," and "right," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the attached circle, or the orientation or positional relationship commonly used when the utility model product is in use. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, these terms indicating orientation or positional relationship should not be construed as limitations on this utility model.

[0044] In the description of this utility model, it should be further noted that, unless otherwise explicitly specified and limited, the terms "set" and "connection" should be interpreted broadly. For example, these terms can refer to a fixed connection, a detachable connection, or an integral connection between components; they can also refer to a mechanical connection or an electrical connection; or they can refer to a direct connection or an indirect connection through an intermediate medium. Those skilled in the art can understand the specific meaning of these terms in this utility model according to the specific circumstances.

Claims

1. A multifunctional reaction column for the solid phase synthesis of phosphorylated cyclic peptides comprising a solid phase extraction column (30) which is placed by a support device, characterized in that: The support device includes a screw (11), on which a threaded sleeve (20) is threadedly fitted. Several stabilizing components are evenly distributed on the outer side of the threaded sleeve (20). The solid phase extraction column (30) has a top plate (303) at the top. A protrusion (301) is fixed at the bottom of the top plate (303). The protrusion (301) is installed in conjunction with the stabilizing components.

2. The multifunctional reaction column for solid phase synthesis of phosphorylated cyclic peptide according to claim 1, characterized in that: The stabilizing component includes an extension rod (201) fixed to the outer wall of the threaded sleeve (20). A support arm (202) is fixed to one end of the extension rod (201), and a sleeve plate (203) is fixed to one side of the support arm (202). A slot (206) that mates with a protrusion (301) is provided on the surface of the sleeve plate (203), and an arc-shaped groove (204) is provided on the inner side of the sleeve plate (203).

3. The multifunctional reaction column for solid phase synthesis of phosphorylated cyclic peptide according to claim 2, characterized in that: The sleeve (203) has extension arms (205) at both ends.

4. The multifunctional reaction column for the solid phase synthesis of phosphorylated cyclic peptides according to claim 3, characterized in that: The bump (301) has three parts that are symmetrically distributed around the central axis.

5. The multifunctional reaction column for the solid phase synthesis of phosphorylated cyclic peptides according to claim 4, characterized in that: The solid phase extraction column (30) has a collection tube (302) at the bottom, and a collar (401) is fitted on the extension rod (201) and the collection tube (302). A corner rod (40) is fixed between the two collars (401).

6. The multifunctional reaction column for the solid phase synthesis of phosphorylated cyclic peptides according to claim 5, characterized in that: The collar (401) on the extension rod (201) is slidably connected.

7. The multifunctional reaction column for the solid phase synthesis of phosphorylated cyclic peptides according to claim 1, characterized in that: The bottom of the screw (11) is fixed with a base (10).

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