A constant temperature and constant flow fluid replenishment device for cell culture

Abstract

This utility model relates to the field of cell culture technology and discloses a constant temperature and constant flow replenishment device for cell culture, including a main body with legs fixedly connected to the bottom of the main body, a culture mechanism on the top of the main body, and a heat preservation mechanism on the outside of the culture mechanism. The culture mechanism includes a closing component, a culture component, a replenishment component, and a liquid addition component. With this constant temperature and constant flow replenishment device for cell culture, the operator only needs to start the water pump to add nutrient solution from the liquid tank to the culture box through the outlet and replenishment pipes, replenishing the nutrient solution for the cultured cells in the culture box. The operation is simple and convenient for operators. Simultaneously, the operator can also add water to the main body of the device. The water is located below the mesh plate and evaporates within the main body, maintaining the humidity of the space inside the main body and preventing the cultured cells in the culture box from being affected by insufficient humidity.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, specifically to a constant temperature and constant flow fluid replenishment device for cell culture. Background Technology

[0002] Cell growth requires a nutrient environment, and the nutrient substrate used to maintain cell growth is called culture medium, which is divided into culture medium and culture solids. During the process of culturing cells in a culture medium, the cells consume the culture medium, so the culture flask needs to be opened occasionally to add more culture medium.

[0003] Existing patent documents: A fluid replenishment device for cell culture (Publication (Announcement) No.: CN 217709484 U; Application No.: 202221615886.7). This application addresses the problem of uneven fluid flow in the culture dish after addition, preventing some areas from absorbing the replenishment and reducing cell culture efficiency. However, the fluid replenishment device in this application lacks a corresponding heat preservation structure during use. During culture, changes in external ambient temperature may cause fluctuations in the culture medium temperature, affecting cell culture results. This device cannot effectively resist external temperature interference and maintain a constant temperature in the culture medium. Although a vibration mechanism disperses the replenishment, it does not ensure a stable replenishment flow rate. Unstable replenishment flow rates may lead to uneven nutrient acquisition by cells, affecting normal cell growth and culture efficiency. Utility Model Content

[0004] The purpose of this invention is to provide a constant temperature and constant flow fluid replenishment device for cell culture, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a constant temperature and constant flow fluid replenishment device for cell culture, comprising a device body, a support leg fixedly connected to the bottom of the device body, a culture mechanism provided on the top of the device body, and a heat preservation mechanism provided on the outside of the culture mechanism.

[0006] The cultivation mechanism includes a closing component, a cultivation component, a liquid replenishment component, and a liquid addition component. The closing component is located inside the right side of the main body of the device, the cultivation component is located inside the main body of the device, the liquid replenishment component is located at the top of the main body of the device, and the liquid addition component is located at the top of the liquid replenishment component.

[0007] The heat insulation mechanism includes a heat insulation component, a positioning component, and a locking component. The heat insulation component is located outside the liquid replenishment component, the positioning component is located on the front and rear sides of the heat insulation component, and the locking component is located outside the positioning component.

[0008] Preferably, the closing component includes a closing cover, which is snapped into the right side of the device body. A connecting hinge is fixedly connected to the top left side of the closing cover, and the connecting hinge is fixedly connected to the top of the device body. A handle is fixedly connected to the right side of the closing cover.

[0009] Preferably, the cultivation component includes a mesh plate, which is fixedly connected to the main body of the device. A cultivation box is disposed above the mesh plate, and a liquid replenishment pipe is disposed above the cultivation box. The liquid replenishment pipe is fixedly connected to the top of the main body of the device.

[0010] Preferably, the liquid replenishment component includes a liquid tank, which is disposed on the top of the main body of the device. A water pump is fixedly connected to the bottom of the liquid tank, and a liquid outlet pipe is fixedly connected to the top of the water pump. The liquid outlet pipe is snapped into the right side of the liquid tank, and a connecting pipe buckle is fixedly connected to the bottom right side of the liquid outlet pipe. The connecting pipe buckle is snapped into the top of the liquid replenishment pipe.

[0011] Preferably, the liquid filling assembly includes a liquid injection port, which is fixedly connected to the top of the liquid tank, and a threaded cap is threadedly connected to the outer ring of the liquid injection port.

[0012] Preferably, the heat insulation component includes a heat insulation cover, which is fitted around the outer ring of the liquid tank. A hole is provided on the top of the heat insulation cover corresponding to the liquid injection port. A groove is provided on the right side of the heat insulation cover corresponding to the liquid outlet pipe. A frame is provided on the outside of the heat insulation cover, and the frame is fixedly connected to the top of the device body.

[0013] Preferably, the positioning component includes a frame plate, which is fixedly connected to the front and rear sides of the main body of the device. A panel is snapped into the outer side of the frame plate, and a locking rod is fixedly connected to the inner side of the panel. The locking rod is snapped into the frame plate, and a locking seat is sleeved on the inner end of the locking rod. The locking seat is fixedly connected to the front and rear sides of the heat insulation cover, and a handle is fixedly connected to the outer side of the panel.

[0014] Preferably, the locking assembly includes a fixed cylinder disposed below the panel and fixedly connected to the outside of the frame plate. A sliding cylinder is slidably connected inside the fixed cylinder, and a compression spring is fixedly connected to the bottom of the sliding cylinder. The compression spring is fixedly connected to the bottom of the fixed cylinder. A positioning rod is fixedly connected to the top of the sliding cylinder, and a sleeve plate is sleeved around the outer ring of the positioning rod. The sleeve plate is fixedly connected to the outside of the panel. A telescopic sliding rod is fixedly connected to the bottom of the sliding cylinder and slidably connected to the bottom of the fixed cylinder. A pull rod is fixedly connected to the bottom of the telescopic sliding rod.

[0015] Compared with the prior art, this utility model provides a constant temperature and constant flow fluid replenishment device for cell culture, which has the following beneficial effects:

[0016] 1. This constant temperature and constant flow replenishment device for cell culture allows operators to simply activate the water pump to add nutrient solution from the liquid tank to the culture box via the outlet and replenishment pipes, thus replenishing the nutrient solution for the cultured cells. The operation is simple and convenient for operators to use. At the same time, operators can also add water to the main body of the device. The water is located below the mesh plate and evaporates inside the main body of the device, maintaining the humidity of the space inside the main body of the device, so that the cultured cells in the culture box will not be affected by insufficient humidity.

[0017] 2. This constant temperature and constant flow nutrient solution replenishment device for cell culture, through its insulated mechanism, uses a heat insulation cover fitted onto the outside of the liquid tank to keep the temperature of the nutrient solution inside the tank from changing significantly due to external environmental influences. This increases the stability of the nutrient solution temperature, preventing cell culture failure due to excessive temperature fluctuations in the added nutrient solution during use. Furthermore, the heat insulation cover is detachable, allowing staff to periodically inspect it and replace it when its insulation effectiveness decreases. The device is simple to operate and convenient for staff to use. Attached Figure Description

[0018] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a front view of the present utility model;

[0020] Figure 2 This is a cross-sectional view of the structure of this utility model;

[0021] Figure 3 This is a schematic diagram showing the main body of the device in open and closed states.

[0022] Figure 4 This is a partial structural cross-sectional view of the fluid replenishment assembly;

[0023] Figure 5 This is a partial structural diagram of the insulation mechanism;

[0024] Figure 6 This is a schematic diagram of part of the insulation mechanism;

[0025] Figure 7 This is a sectional view of the component structure.

[0026] In the diagram: 1. Cultivation mechanism; 11. Closure assembly; 1101. Closure cover; 1102. Connecting hinge; 1103. Handle; 12. Cultivation assembly; 1201. Mesh plate; 1202. Cultivation box; 1203. Liquid replenishment tube; 13. Liquid replenishment assembly; 1301. Liquid tank; 1302. Water pump; 1303. Liquid outlet tube; 1304. Connecting tube buckle; 14. Liquid addition assembly; 1401. Liquid inlet; 1402. Threaded cap; 2. Insulation mechanism; 21. Thermal insulation component; 2101. Thermal insulation cover; 2102. Sleeve frame; 22. Positioning component; 2201. Shelf plate; 2202. Panel; 2203. Locking rod; 2204. Locking seat; 2205. Handle; 23. Locking component; 2301. Fixing cylinder; 2302. Slide cylinder; 2303. Compression spring; 2304. Positioning rod; 2305. Sleeve plate; 2306. Telescopic slide rod; 2307. Pull rod; 3. Main body of the device; 4. Support leg. Detailed Implementation

[0027] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0028] This utility model provides a technical solution: Example 1

[0029] Combination Figures 1 to 4 A constant temperature and constant flow fluid replenishment device for cell culture includes a device body 3, a support leg 4 fixedly connected to the bottom of the device body 3, a culture mechanism 1 provided on the top of the device body 3, and a heat preservation mechanism 2 provided on the outside of the culture mechanism 1.

[0030] The cultivation mechanism 1 includes a closing component 11, a cultivation component 12, a liquid replenishment component 13, and a liquid addition component 14. The closing component 11 is located inside the right side of the main body 3 of the device, the cultivation component 12 is located inside the main body 3 of the device, the liquid replenishment component 13 is located at the top of the main body 3 of the device, and the liquid addition component 14 is located at the top of the liquid replenishment component 13.

[0031] Closure component 11 includes a closure cover 1101, which is snapped into the right side of the device body 3. A connecting hinge 1102 is fixedly connected to the top left side of the closure cover 1101, and the connecting hinge 1102 is fixedly connected to the top of the device body 3. A handle 1103 is fixedly connected to the right side of the closure cover 1101. The cultivation component 12 includes a mesh plate 1201, which is fixedly connected to the device body 3. A cultivation box 1202 is disposed above the mesh plate 1201, and a liquid replenishment tube 1203 is disposed above the cultivation box 1202. The liquid replenishment tube 1203 is fixedly connected to the top of the device body 3 for liquid replenishment. Component 13 includes a liquid tank 1301, which is located on the top of the main body 3 of the device. A water pump 1302 is fixedly connected to the bottom of the liquid tank 1301. A liquid outlet pipe 1303 is fixedly connected to the top of the water pump 1302. The liquid outlet pipe 1303 is snapped into the right side of the liquid tank 1301. A connecting pipe buckle 1304 is fixedly connected to the bottom right side of the liquid outlet pipe 1303. The connecting pipe buckle 1304 is snapped into the top of the replenishment pipe 1203. The liquid addition component 14 includes an injection port 1401, which is fixedly connected to the top of the liquid tank 1301. A threaded cap 1402 is threadedly connected to the outer ring of the injection port 1401.

[0032] Furthermore, staff only need to activate the water pump 1302 to add the nutrient solution in the liquid tank 1301 to the culture box 1202 through the liquid outlet pipe 1303 and the liquid replenishment pipe 1203, thus replenishing the nutrient solution for the cultured cells in the culture box 1202. The operation is simple and convenient for staff to use. At the same time, staff can also add water to the main body 3 of the device. The water is located below the mesh plate 1201 and evaporates inside the main body 3 of the device, maintaining the humidity of the space inside the main body 3 of the device, so that the cultured cells in the culture box 1202 will not be affected by insufficient humidity. Example 2

[0033] See Figures 1 to 7 Furthermore, based on Embodiment 1, the heat preservation mechanism 2 includes a heat insulation component 21, a positioning component 22, and a locking component 23. The heat insulation component 21 is disposed on the outside of the liquid replenishment component 13, the positioning component 22 is disposed on the front and rear sides of the heat insulation component 21, and the locking component 23 is disposed on the outside of the positioning component 22.

[0034] The insulation component 21 includes an insulation cover 2101, which is fitted around the outer ring of the liquid tank 1301. The top of the insulation cover 2101 has a hole corresponding to the injection port 1401, and the right side of the insulation cover 2101 has a groove corresponding to the outlet pipe 1303. A frame 2102 is provided on the outer side of the insulation cover 2101 and is fixedly connected to the top of the device body 3. The positioning component 22 includes a frame plate 2201, which is fixedly connected to the front and rear sides of the device body 3. A panel 2202 is snapped into the outer side of the frame plate 2201, and a locking rod 2203 is fixedly connected to the inner side of the panel 2202. The locking rod 2203 is snapped into the frame plate 2201, and a locking seat 2204 is sleeved on the inner end of the locking rod 2203. The locking seat 2204 is fixedly connected to the front and rear sides of the insulation cover 2101. A handle 2205 is fixedly connected to the outside. The locking assembly 23 includes a fixed cylinder 2301, which is located below the panel 2202 and is fixedly connected to the outside of the frame 2201. A slide cylinder 2302 is slidably connected inside the fixed cylinder 2301. A compression spring 2303 is fixedly connected to the bottom of the slide cylinder 2302 and is fixedly connected to the bottom of the fixed cylinder 2301. A positioning rod 2304 is fixedly connected to the top of the slide cylinder 2302. A sleeve plate 2305 is sleeved around the outer ring of the positioning rod 2304 and is fixedly connected to the outside of the panel 2202. A telescopic slide rod 2306 is fixedly connected to the bottom of the slide cylinder 2302 and is slidably connected to the bottom of the fixed cylinder 2301. A pull rod 2307 is fixedly connected to the bottom of the telescopic slide rod 2306.

[0035] Furthermore, the insulation cover 2101 is fitted onto the outside of the liquid tank 1301 to insulate the nutrient solution inside the liquid tank 1301, preventing significant changes in the temperature of the nutrient solution due to external environmental influences. This increases the stability of the nutrient solution temperature, ensuring that the cultured cells in the culture box 1202 will not fail due to excessive temperature fluctuations in the added nutrient solution during use, thus increasing the stability of the cell culture process. Additionally, the insulation cover 2101 is detachable, allowing staff to periodically inspect it and replace it when its insulation effect diminishes. The operation is simple and convenient for staff to use.

[0036] In actual operation, when this device is used, the staff first adds water to the main body 3 of the device so that the water level is lower than the mesh plate 1201. Then, the staff places the cells to be cultured into the culture box 1202 and places the culture box 1202 above the mesh plate 1201. Then, the closing cover 1101 is closed. At this time, the space inside the main body 3 is isolated from the outside, and the water inside the main body 3 gradually evaporates, increasing the humidity inside the main body 3, which is suitable for cell culture. When it is necessary to replenish the nutrient solution in the culture box 1202, the staff only needs to start the water pump 1302. The water pump 1302 starts to pump the nutrient solution in the liquid tank 1301, so that the nutrient solution drips into the culture box 1202 through the liquid outlet pipe 1303 and the liquid replenishment pipe 1203. After enough nutrient solution is added, the water pump 1302 is turned off. At this time, the liquid replenishment treatment in the culture box 1202 is completed.

[0037] When it is necessary to disassemble the heat insulation cover 2101, the operator first presses the pull rods 2307 on both the front and rear sides downwards. The pull rods 2307 move downwards, which in turn moves the slide cylinder 2302 downwards via the telescopic slide rod 2306. The slide cylinder 2302 moves downwards, which in turn moves the positioning rod 2304 downwards. The positioning rod 2304 moves downwards and retracts into the fixed cylinder 2301, disengaging from the sleeve plate 2305. Then, the operator pulls the handle 2205 outwards. The handle 2205 moves outwards, which in turn moves the locking rod 2203 outwards via the insert plate 2202. The locking rod 2203 moves outwards, disengaging from the frame plate 2201 and the mounting base 2204. At this point, the locking of the heat insulation cover 2101 is released. The operator can then move the heat insulation cover 2101 upwards to disassemble it. Subsequently, the operator can inspect and replace the heat insulation cover 2101, and the liquid tank 1301 can also be replaced.

Claims

1. A constant temperature and constant flow liquid supplementing device for cell culture, comprising a device main body (3), characterized in that: The device body (3) is fixedly connected to the bottom of the support leg (4), the device body (3) is provided with a cultivation mechanism (1) at the top, and the cultivation mechanism (1) is provided with a heat preservation mechanism (2) on the outside. The cultivation mechanism (1) includes a closing component (11), a cultivation component (12), a liquid replenishment component (13), and a liquid addition component (14). The closing component (11) is located inside the right side of the main body (3), the cultivation component (12) is located inside the main body (3), the liquid replenishment component (13) is located at the top of the main body (3), and the liquid addition component (14) is located at the top of the liquid replenishment component (13). The heat preservation mechanism (2) includes a heat insulation component (21), a positioning component (22) and a locking component (23). The heat insulation component (21) is located outside the liquid replenishment component (13). The positioning component (22) is located on the front and rear sides of the heat insulation component (21). The locking component (23) is located outside the positioning component (22).

2. The constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The closing component (11) includes a closing cover (1101), which is snapped into the right side of the device body (3). A connecting hinge (1102) is fixedly connected to the top left side of the closing cover (1101), and the connecting hinge (1102) is fixedly connected to the top of the device body (3). A handle (1103) is fixedly connected to the right side of the closing cover (1101).

3. The constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The cultivation component (12) includes a mesh plate (1201), which is fixedly connected to the main body (3) of the device. A cultivation box (1202) is provided above the mesh plate (1201), and a liquid replenishment pipe (1203) is provided above the cultivation box (1202). The liquid replenishment pipe (1203) is fixedly connected to the top of the main body (3) of the device.

4. The constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The replenishment component (13) includes a liquid tank (1301), which is located on the top of the main body (3) of the device. A water pump (1302) is fixedly connected to the bottom of the liquid tank (1301), and an outlet pipe (1303) is fixedly connected to the top of the water pump (1302). The outlet pipe (1303) is snapped into the right side of the liquid tank (1301), and a connecting pipe buckle (1304) is fixedly connected to the bottom right side of the outlet pipe (1303). The connecting pipe buckle (1304) is snapped into the top of the replenishment pipe (1203).

5. A constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The liquid filling assembly (14) includes a liquid inlet (1401), which is fixedly connected to the top of the liquid tank (1301), and a threaded cap (1402) is threadedly connected to the outer ring of the liquid inlet (1401).

6. The constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The heat insulation component (21) includes a heat insulation cover (2101), which is fitted around the outer ring of the liquid tank (1301). The top of the heat insulation cover (2101) has a hole corresponding to the position of the liquid injection port (1401), and the right side of the heat insulation cover (2101) has a groove corresponding to the position of the liquid outlet pipe (1303). A frame (2102) is provided on the outside of the heat insulation cover (2101), and the frame (2102) is fixedly connected to the top of the main body (3) of the device.

7. A constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The positioning component (22) includes a frame plate (2201), which is fixedly connected to the front and rear sides of the main body (3) of the device. A panel (2202) is snapped into the outer side of the frame plate (2201). A locking rod (2203) is fixedly connected to the inner side of the panel (2202). The locking rod (2203) is snapped into the frame plate (2201). A locking seat (2204) is sleeved on the inner end of the locking rod (2203). The locking seat (2204) is fixedly connected to the front and rear sides of the heat insulation cover (2101). A handle (2205) is fixedly connected to the outer side of the panel (2202).

8. The constant temperature and constant flow fluid replenishment device for cell culture according to claim 1, characterized in that: The locking assembly (23) includes a fixed cylinder (2301), which is located below the panel (2202). The fixed cylinder (2301) is fixedly connected to the outside of the frame (2201). A sliding cylinder (2302) is slidably connected inside the fixed cylinder (2301). A compression spring (2303) is fixedly connected to the bottom of the sliding cylinder (2302). The compression spring (2303) is fixedly connected to the bottom inside the fixed cylinder (2301). A positioning rod (2304) is fixedly connected to the top of the sliding cylinder (2302). A sleeve plate (2305) is sleeved on the outer ring of the positioning rod (2304). The sleeve plate (2305) is fixedly connected to the outside of the panel (2202).

9. A constant temperature and constant flow fluid replenishment device for cell culture according to claim 8, characterized in that: The bottom of the slide cylinder (2302) is fixedly connected to a telescopic slide rod (2306), which is slidably connected to the bottom of the fixed cylinder (2301). The bottom of the telescopic slide rod (2306) is fixedly connected to a pull rod (2307).

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