A fully automated temperature-controlled oscillating cell culture device

Abstract

This invention provides a fully automatic temperature-controlled oscillating cell culture device, relating to the field of constant temperature oscillating cell culture incubator technology. The device includes a constant temperature oscillating incubator body, with a lifting mechanism fixedly connected to the top of the body. The lifting mechanism and a conveying mechanism are installed on the surface of the incubator body. The lifting mechanism moves several injection heads from the bottom to directly above the culture container. Then, the pump is activated, and the pump delivers the culture medium from the storage tank to the injection heads via hoses and distribution pipes. Finally, the injection heads deliver the culture medium to the culture container for replenishment. This eliminates the need for frequent manual addition by opening the sealed door, effectively preventing external microorganisms, dust, and other impurities from entering the incubator, contaminating the cell culture environment, and increasing the risk of cell contamination.

Description

Technical Field

[0001] This application relates to the field of constant temperature oscillating cell culture chamber technology, and in particular to a fully automatic temperature-controlled oscillating cell culture device. Background Technology

[0002] In the fields of biomedical research and cell engineering, cell culture is a fundamental and crucial technology. The stability and automation level of the culture environment directly determine the success or failure and efficiency of experiments. Although existing cell culture devices have initially achieved temperature control and oscillation functions, the following core problems have been exposed during technological iterations.

[0003] During cell culture, nutrients in the culture medium are continuously consumed as cells grow and metabolize, requiring timely replenishment to maintain normal cell growth. Current cell culture equipment typically requires manual addition of culture medium by opening the incubator door. This method has significant drawbacks: First, frequent door opening disrupts the stable temperature, humidity, and gas environment within the incubator, causing fluctuations and affecting cell growth. Second, external microorganisms, dust, and other impurities can easily enter the incubator during door opening, contaminating the cell culture environment and increasing the risk of cell contamination. If cells are contaminated, the entire culture experiment may fail, resulting in a waste of time and resources. Utility Model Content

[0004] This application provides a fully automated temperature-controlled oscillating cell culture device to solve the problems of constant temperature oscillating cell culture incubators.

[0005] This application provides a fully automated temperature-controlled oscillating cell culture device, including a constant temperature oscillating incubator body. A lifting mechanism is fixedly connected to the top of the constant temperature oscillating incubator body, and a culture medium storage tank is installed on one side of the constant temperature oscillating incubator body. A conveying mechanism is connected through the top of the culture medium storage tank.

[0006] The lifting mechanism includes a hydraulic rod fixedly connected to the top of the constant temperature shaking incubator body, and a connecting frame is fixedly connected to the output end of the hydraulic rod;

[0007] The delivery mechanism includes a pump body that is connected to the top of the culture medium storage tank. A hose is connected to the output end of the pump body, and a diverter tube is connected to the end of the hose. Several injection heads are connected to the surface of the diverter tube along the axial direction.

[0008] Preferably, support blocks are fixedly connected to both sides of the diversion pipe, and the bottom of the support blocks is fixedly connected to the top surface of the connecting frame, so that the support blocks can easily support the diversion pipe.

[0009] Preferably, the constant temperature shaking incubator body has a culture chamber inside, and a placement plate is installed inside the culture chamber to facilitate the placement of culture containers.

[0010] Preferably, the surface of the placement plate has six placement slots, and a silicone support pad is installed inside the placement slot. The silicone support pad facilitates the compression and fixation of the culture tube.

[0011] Preferably, the top of the culture medium storage tank is provided with a liquid injection port, and a scale plate is installed on one side of the culture medium storage tank for easy observation of the capacity.

[0012] Preferably, a sealed door is hinged to one side of the main body of the constant temperature shaking incubator, and an observation window is provided on the surface of the sealed door to facilitate observation of the internal situation from the outside.

[0013] Preferably, the bottom of the constant temperature oscillation incubator body is fixedly connected with connecting columns in a rectangular array, and silicone shock-absorbing blocks are installed at the bottom of the connecting columns to facilitate buffering and shock absorption. Beneficial effects

[0014] To address the issues with constant-temperature oscillating cell culture incubators, a lifting mechanism and a delivery mechanism are installed on the surface of the main body of the incubator. The lifting mechanism moves several injection heads from the bottom to directly above the culture container. Then, the pump is activated, and the pump delivers the culture medium from the storage tank to the injection heads via hoses and distribution tubes. Finally, the injection heads deliver the culture medium to the culture container for replenishment. This eliminates the need for staff to frequently open the sealed door for manual addition, effectively preventing external microorganisms, dust, and other impurities from entering the incubator, contaminating the cell culture environment, and increasing the risk of cell contamination.

[0015] The above description is merely an overview of the technical solutions of the embodiments of this application. In order to better understand the technical means of the embodiments of this application and to implement them in accordance with the contents of the specification, and to make the above and other objects, features and advantages of the embodiments of this application more obvious and understandable, specific implementation methods of this application are described below. Attached Figure Description

[0016] To more clearly illustrate the technical solutions of the embodiments of this application, the drawings used in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of a fully automatic temperature-controlled oscillating cell culture device according to the present invention.

[0018] Figure 2 This is a schematic diagram of the conveying mechanism of a fully automatic temperature-controlled oscillating cell culture device according to the present invention.

[0019] Figure 3 This is a schematic diagram of the main structure of the constant temperature oscillation incubator of a fully automatic temperature-controlled oscillation cell culture device according to this utility model.

[0020] Figure 4 This is a schematic diagram of the lifting mechanism of a fully automatic temperature-controlled oscillating cell culture device according to the present invention.

[0021] Explanation of reference numerals in the attached figures:

[0022] 1. Main body of constant temperature shaking incubator; 2. Lifting mechanism; 201. Hydraulic rod; 202. Connecting frame; 3. Culture medium storage tank; 4. Delivery mechanism; 401. Pump body; 402. Hoses; 403. Diverter tube; 404. Injection head; 5. Support block; 6. Culture chamber; 7. Placement plate; 8. Silicone support pad; 9. Injection port; 10. Scale plate; 11. Sealing door; 12. Observation window; 13. Connecting column; 14. Silicone shock absorber block. Detailed Implementation

[0023] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used herein in the specification of the application is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms “comprising” and “having”, and any variations thereof, in the specification, claims and drawings of this application are intended to cover non-exclusive inclusion.

[0025] The term "embodiment" as used herein means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of the phrase "embodiment" in various places throughout the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment mutually exclusive with other embodiments. It will be explicitly and implicitly understood by those skilled in the art that the embodiments described herein can be combined with other embodiments.

[0026] The directional terms appearing in the following description refer to the directions shown in the figures and are not intended to limit the specific structure of this application. For example, in the description of this application, terms such as "center," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," and "circumferential" indicate the orientation or positional relationship based on the orientation or positional relationship shown in the figures. They are used only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0027] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, "connection" or "joining" in mechanical structures can refer to a physical connection, such as a fixed connection, for example, a connection fixed by fasteners, such as a connection fixed by screws, bolts, or other fasteners; a physical connection can also be a detachable connection, such as a snap-fit ​​or interlocking connection; a physical connection can also be an integral connection, such as a connection formed by welding, bonding, or integral molding. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0028] To enable those skilled in the art to better understand the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the accompanying drawings.

[0029] This utility model provides, for example Figure 1-4 The illustrated fully automated temperature-controlled oscillating cell culture device includes a constant temperature oscillating incubator body 1, a lifting mechanism 2 fixedly connected to the top of the constant temperature oscillating incubator body 1, a culture medium storage tank 3 installed on one side of the constant temperature oscillating incubator body 1, and a conveying mechanism 4 connected through the top of the culture medium storage tank 3.

[0030] The lifting mechanism 2 includes a hydraulic rod 201 fixedly connected to the top of the constant temperature shaking incubator body 1, and a connecting frame 202 fixedly connected to the output end of the hydraulic rod 201;

[0031] The delivery mechanism 4 includes a pump body 401 that is connected to the top of the culture medium storage tank 3. The output end of the pump body 401 is connected to a hose 402. The end of the hose 402 is connected to a diverter pipe 403. Several injection heads 404 are connected to the surface of the diverter pipe 403 along the axial direction.

[0032] Among them, support blocks 5 are fixedly connected to both sides of the diversion pipe 403, and the bottom of the support block 5 is fixedly connected to the top surface of the connecting frame 202.

[0033] Support block 5 facilitates support of the diversion pipe 403.

[0034] The constant temperature oscillating incubator body 1 has a culture chamber 6 inside, and a placement plate 7 is installed inside the culture chamber 6.

[0035] The placement plate 7 facilitates the placement of culture containers.

[0036] The surface of the placement plate 7 has six placement slots, and silicone support pads 8 are installed inside the placement slots.

[0037] The silicone support pad 8 facilitates the compression and fixation of the culture tubes.

[0038] The culture medium storage tank 3 has an injection port 9 on its top and a scale plate 10 installed on one side of its side.

[0039] The scale plate 10 makes it easy to observe the capacity.

[0040] One side of the constant temperature shaking incubator body 1 is hinged to a sealing door 11, and an observation window 12 is provided on the surface of the sealing door 11.

[0041] The observation window 12 facilitates observation of the internal situation from the outside.

[0042] The bottom of the constant temperature oscillating incubator body 1 is fixedly connected with connecting columns 13 in a rectangular array, and silicone shock-absorbing blocks 14 are installed at the bottom of the connecting columns 13.

[0043] Silicone shock absorber block 14 facilitates cushioning and shock absorption.

[0044] Working principle: When using this fully automatic temperature-controlled oscillating cell culture device, a lifting mechanism 2 and a conveying mechanism 4 are installed on the surface of the main body 1 of the constant temperature oscillating incubator. The lifting mechanism 2 moves several injection heads 404 at the bottom to the top of the culture container. Then, the pump body 401 is started. The pump body 401 delivers the culture medium inside the culture medium storage tank 3 to the injection head 404 through the hose 402 and the diversion pipe 403. Finally, the injection head 404 delivers the culture medium to the culture container to replenish the culture medium. There is no need for the staff to frequently open the sealed door 11 to add the medium manually.

[0045] The above-described embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this application.

Claims

1. A fully automatic temperature-controlled oscillation type cell culture device comprising a constant-temperature oscillation incubator main body (1), characterized in that: The top of the constant temperature oscillating incubator body (1) is fixedly connected to a lifting mechanism (2), and a culture medium storage tank (3) is installed on one side of the constant temperature oscillating incubator body (1). The top of the culture medium storage tank (3) is connected to a conveying mechanism (4). The lifting mechanism (2) includes a hydraulic rod (201) fixedly connected to the top of the constant temperature oscillating incubator body (1), and a connecting frame (202) is fixedly connected to the output end of the hydraulic rod (201). The delivery mechanism (4) includes a pump body (401) that is connected to the top of the culture medium storage tank (3). The output end of the pump body (401) is connected to a hose (402). The end of the hose (402) is connected to a shunt pipe (403). Several injection heads (404) are connected to the surface of the shunt pipe (403) along the axial direction.

2. The fully automated temperature-controlled oscillating cell culture device according to claim 1, characterized in that: Both sides of the diversion pipe (403) are fixedly connected to support blocks (5), and the bottom of the support blocks (5) is fixedly connected to the top surface of the connecting frame (202).

3. The fully automated temperature-controlled oscillating cell culture device according to claim 1, characterized in that: The constant temperature oscillating incubator body (1) has a culture chamber (6) inside, and a placement plate (7) is installed inside the culture chamber (6).

4. The fully automated temperature-controlled oscillating cell culture device according to claim 3, characterized in that: The surface of the placement plate (7) is provided with six placement slots, and a silicone support pad (8) is installed inside the placement slot.

5. The fully automated temperature-controlled oscillating cell culture device according to claim 1, characterized in that: The top of the culture medium storage tank (3) is provided with a liquid injection port (9), and a scale plate (10) is installed on one side of the culture medium storage tank (3).

6. The fully automated temperature-controlled oscillating cell culture device according to claim 1, characterized in that: The main body (1) of the constant temperature shaking incubator is hinged to a sealing door (11) on one side, and an observation window (12) is provided on the surface of the sealing door (11).

7. The fully automated temperature-controlled oscillating cell culture device according to claim 1, characterized in that: The bottom of the constant temperature oscillation incubator body (1) is fixedly connected with connecting columns (13) in a rectangular array, and silicone shock-absorbing blocks (14) are installed at the bottom of the connecting columns (13).

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