A high-efficiency yeast nucleotide extraction system

Abstract

The utility model discloses a kind of yeast nucleotide high-efficiency extraction systems, including autolysis tank, agitator and circulation module are equipped on the autolysis tank, material in autolysis tank is circulated by circulation module, online near-infrared instrument is equipped on the feed end of circulation module, circulation module discharge end is communicated with autolysis tank after passing through air supplement module and enzyme preparation supplement module in turn, increase online near-infrared instrument, improve detection efficiency, with the function of online detection nucleotide content;Increase air supplement module, air is inhaled, accelerate yeast nucleic acid oxidation and decomposition, improve nucleotide extraction efficiency;Increase enzyme preparation supplement module, according to time and nucleotide content in tank, automatically flow enzyme preparation, multi-section enzyme, can effectively improve enzymatic extraction efficiency, reduce enzyme preparation loss;Increase the flow mixer, so that material, enzyme preparation, air three are fully mixed, accelerate yeast nucleic acid oxidation and decomposition, improve nucleotide extraction efficiency.

Description

Technical Field

[0001] This utility model relates to the technical field of yeast production and processing equipment, and in particular to a high-efficiency yeast nucleotide extraction system. Background Technology

[0002] Existing yeast nucleotide extraction systems typically use autodissolving tanks and circulating pipelines and pumps to form a simple circulation. The material enters the circulating pipeline through the outlet of the autodissolving tank, and under the drive of the circulating pump, it returns to the autodissolving tank through the circulating pipeline to complete the circulation. During the process, the material is stirred by a circulating agitator.

[0003] This extraction system suffers from low extraction efficiency, high enzyme loss, low automation level, and insufficient process refinement. Utility Model Content

[0004] This invention provides a high-efficiency yeast nucleotide extraction system, which aims to solve the problems of low extraction efficiency, high enzyme loss, low automation level, and insufficient process refinement in the existing yeast nucleotide extraction systems.

[0005] To solve the above-mentioned technical problems, the technical solution adopted by this utility model is as follows:

[0006] A high-efficiency yeast nucleotide extraction system includes an autolysis tank, which is equipped with a stirrer and a circulation module. The material in the autolysis tank is circulated through the circulation module. The feed end of the circulation module is equipped with an online near-infrared spectrometer. The discharge end of the circulation module is connected to the autolysis tank after passing through a gas replenishment module and an enzyme replenishment module in sequence.

[0007] Preferably, the circulation module includes a circulation pipeline that forms a circulation with the autolytic tank, and a variable frequency centrifugal pump is provided on the circulation pipeline.

[0008] More preferably, the online near-infrared instrument is installed on the circulation pipeline on the feed side of the variable frequency circulating centrifugal pump, and the gas replenishment module and enzyme replenishment module are installed on the circulation pipeline on the discharge side of the variable frequency circulating centrifugal pump.

[0009] Furthermore, the gas replenishment module includes a three-way mixer. The outlet of the circulation pipeline is connected to one of the inlets of the three-way mixer. The other inlet of the three-way mixer is connected to a variable frequency fan through a ventilation pipe. The variable frequency fan is connected to an external clean air source. The outlet of the three-way mixer is connected to the inlet of the enzyme supplementation module.

[0010] Furthermore, a one-way valve is provided on the side of the ventilation duct near a three-way mixer.

[0011] Furthermore, the enzyme supplement module includes a two-stage three-way mixer. One inlet of the two-stage three-way mixer is connected to the outlet of the one-stage three-way mixer. The other inlet of the two-stage three-way mixer is connected to the enzyme preparation storage tank via a feed pipe. An electromagnetic flow valve and an enzyme preparation addition pump are sequentially installed on the feed pipe along the enzyme preparation flow direction. The outlet of the two-stage three-way mixer is connected to the autolysis tank.

[0012] Specifically, a one-way valve is provided on the side of the feed tube near the two-stage three-way mixer.

[0013] In detail, the online near-infrared instrument is used to monitor and detect nucleotide content online, and the online near-infrared instrument is linked with the enzyme supplement module through an external controller.

[0014] More specifically, the online near-infrared instrument is linked with an electromagnetic flow valve and an enzyme preparation addition pump via an external controller.

[0015] The beneficial effects of this utility model are:

[0016] 1. This patent adds a frequency converter to the circulating centrifugal pump, which can reduce the frequency and save energy in the later stage of extraction;

[0017] 2. An online near-infrared instrument has been added, which improves detection efficiency and has the function of online detection of nucleotide content, making it convenient to link with enzyme preparation addition;

[0018] 3. An air supply module has been added, which introduces air through a variable frequency fan to accelerate the oxidative decomposition of yeast nucleic acids and improve nucleotide extraction efficiency;

[0019] 4. An enzyme supplementation module has been added. Based on the time and the nucleotide content in the tank, the enzyme preparation is automatically added in a multi-stage manner, which can effectively improve the efficiency of enzymatic extraction and reduce enzyme preparation loss.

[0020] 5. A mixer has been added to ensure thorough mixing of materials, enzymes, and air, thereby accelerating the oxidative decomposition of yeast nucleic acids and improving nucleotide extraction efficiency. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the system connection of this utility model;

[0022] In the diagram: 1. Autodissolving tank; 2. Stirrer;

[0023] 3. Circulation module; 301. Circulation pipeline; 302. Variable frequency centrifugal pump;

[0024] 4. Air supply module; 401. One-stage three-way mixer; 402. Variable frequency fan; 403. Ventilation duct;

[0025] 5. Enzyme supplementation module; 501. Enzyme storage tank; 502. Enzyme addition pump; 503. Electromagnetic flow valve; 504. Flow tube; 505. Two-stage three-way mixer;

[0026] 6. Online near-infrared spectroscopy; 7. One-way valve. Detailed Implementation

[0027] The embodiments will be further described below with reference to the accompanying drawings.

[0028] like Figure 1 As shown in the preferred embodiment 1, a high-efficiency yeast nucleotide extraction system includes an autolysis tank 1, which is equipped with a stirrer 2 and a circulation module 3. The material in the autolysis tank 1 is circulated through the circulation module 3. The feed end of the circulation module 3 is equipped with an online near-infrared instrument 6. The discharge end of the circulation module 3 is connected to the autolysis tank 1 after passing through a gas replenishment module 4 and an enzyme replenishment module 5 in sequence.

[0029] The circulation module 3 includes a circulation pipeline 301 that forms a circulation with the autolytic tank 1, and a variable frequency circulating centrifugal pump 302 is provided on the circulation pipeline 301.

[0030] The online near-infrared instrument 6 is installed on the circulation pipeline 301 on the feed side of the variable frequency circulating centrifugal pump 302, and the gas replenishment module 4 and enzyme replenishment module 5 are installed on the circulation pipeline 301 on the discharge side of the variable frequency circulating centrifugal pump 302. By adding a frequency converter to the circulating centrifugal pump, the frequency can be reduced in the later stage of extraction to save energy.

[0031] The gas replenishment module 4 includes a three-way mixer 401. The outlet of the circulation pipe 301 is connected to one of the inlets of the three-way mixer 401. The other inlet of the three-way mixer 401 is connected to a variable frequency fan 402 via a ventilation pipe 403. The variable frequency fan 402 is connected to an external clean air source. The outlet of the three-way mixer 401 is connected to the inlet of the enzyme supplementation module 5. The three-way mixer 401 ensures thorough mixing of gas and material, and by increasing air intake, it improves the oxidative decomposition efficiency of yeast nucleic acids, thereby increasing the nucleotide extraction efficiency.

[0032] A one-way valve 7 is provided on the side of the ventilation pipe 403 near the three-way mixer 401 to prevent backflow.

[0033] The enzyme supplementation module 5 includes a two-stage three-way mixer 505. One inlet of the two-stage three-way mixer 505 is connected to the outlet of a one-stage three-way mixer 401, and the other inlet of the two-stage three-way mixer 505 is connected to the enzyme preparation storage tank 501 via a feed pipe 504. An electromagnetic flow valve 503 and an enzyme preparation addition pump 502 are sequentially installed along the enzyme preparation flow direction on the feed pipe 504. The outlet of the two-stage three-way mixer 505 is connected to the autolysis tank 1. The two-stage three-way mixer 505 ensures thorough mixing of the material after mixing with the gas and the added enzyme preparation. The electromagnetic flow valve 503 and the enzyme preparation addition pump 502 control the amount and frequency of addition, forming a multi-stage, quantitative addition, which can effectively improve the enzymatic extraction efficiency and reduce enzyme preparation loss.

[0034] A one-way valve 7 is provided on the side of the feed tube 504 near the two-stage three-way mixer 505 to prevent backflow.

[0035] The online near-infrared instrument 6 is used to monitor the content of nucleotides online. The online near-infrared instrument 6 is linked with the enzyme supplement module 5 through an external controller.

[0036] The online near-infrared instrument 6 is linked with the electromagnetic flow valve 503 and the enzyme preparation addition pump 502 via an external controller. Simultaneously, it monitors and controls the amount and frequency of enzyme preparation addition in real time, effectively improving enzymatic extraction efficiency and reducing enzyme preparation loss.

[0037] As a preferred embodiment 2, the enzyme preparation feeding is controlled in the following steps:

[0038] After entering the nucleotide extraction stage, enzyme addition is set to segment X. The interval between enzyme additions is H = total extraction time / X. The required nucleotide content for each segment is J = total nucleotide content requirement / X. The additional enzyme amount is B. The nucleotide content detected by near-infrared spectroscopy is K. If K < J for each enzyme addition, then B = (JK) / J × 100% × Y. If K ≥ J for each enzyme addition, then B = (KJ) / J × (-1) × 100% × Y. The amount of enzyme added each time is Y = total enzyme amount added in this process / X + B. Therefore, the enzyme addition method is as follows: immediately after entering the nucleotide extraction stage, the first enzyme addition is Y = total enzyme amount added in this process / X + B liters (the first B = 0). Enzyme addition is performed every H time interval, Y liters = total enzyme amount added in this process / X + B (B is calculated according to the conditions).

[0039] The working principle of this utility model:

[0040] Material from autolysis tank 1 enters circulation module 3, and then from the outlet of circulation module 3 into air replenishment module 4. There, it is thoroughly mixed with clean air in a first-stage three-way mixer 401, accelerating the oxidative decomposition of yeast nucleic acid and improving nucleotide extraction efficiency. The mixed material then enters enzyme preparation module, where it is thoroughly mixed with enzyme preparation in a second-stage three-way mixer 505. This ensures thorough mixing of material, enzyme preparation, and air, further accelerating the oxidative decomposition of yeast nucleic acid and improving nucleotide extraction efficiency. The addition of an online near-infrared instrument enhances detection efficiency, enabling online detection of nucleotide content. This facilitates linkage with enzyme preparation addition, automatically adding enzyme preparation based on time and nucleotide content in the tank. This multi-stage enzyme addition effectively improves enzymatic extraction efficiency and reduces enzyme preparation loss.

Claims

1. A high-efficiency yeast nucleotide extraction system, comprising an autolysis tank (1), wherein the autolysis tank (1) is equipped with a stirrer (2) and a circulation module (3), and the material in the autolysis tank (1) is circulated through the circulation module (3), characterized in that, The feed end of the circulation module (3) is equipped with an online near-infrared instrument (6), and the discharge end of the circulation module (3) is connected to the autolytic tank (1) after passing through the gas replenishment module (4) and the enzyme replenishment preparation module (5) in sequence.

2. The yeast nucleotide high-efficiency extraction system according to claim 1, characterized in that, The circulation module (3) includes a circulation pipeline (301) that forms a circulation with the autolytic tank (1), and a variable frequency circulation centrifugal pump (302) is provided on the circulation pipeline (301).

3. The yeast nucleotide high-efficiency extraction system according to claim 2, characterized in that, The online near-infrared instrument (6) is installed on the circulation pipeline (301) on the feed end side of the variable frequency circulating centrifugal pump (302), and the gas replenishment module (4) and enzyme replenishment module (5) are installed on the circulation pipeline (301) on the discharge end side of the variable frequency circulating centrifugal pump (302).

4. The yeast nucleotide high-efficiency extraction system according to claim 3, characterized in that, The gas replenishment module (4) includes a three-way mixer (401), the outlet of the circulation pipeline (301) is connected to one of the feed ends of the three-way mixer (401), the other feed end of the three-way mixer (401) is connected to the variable frequency fan (402) through the ventilation pipe (403), the variable frequency fan (402) is connected to an external clean air source, and the outlet of the three-way mixer (401) is connected to the feed end of the enzyme supplementation module (5).

5. The yeast nucleotide high-efficiency extraction system according to claim 4, characterized in that, A one-way valve (7) is provided on the side of the ventilation pipe (403) near the three-way mixer (401).

6. The yeast nucleotide high-efficiency extraction system according to claim 4, characterized in that, The enzyme supplement module (5) includes a two-stage three-way mixer (505). One inlet of the two-stage three-way mixer (505) is connected to the outlet of the one-stage three-way mixer (401). The other inlet of the two-stage three-way mixer (505) is connected to the enzyme storage tank (501) through a feed pipe (504). An electromagnetic flow valve (503) and an enzyme addition pump (502) are sequentially provided on the feed pipe (504) along the enzyme flow direction. The outlet of the two-stage three-way mixer (505) is connected to the autolytic tank (1).

7. The yeast nucleotide high-efficiency extraction system according to claim 6, characterized in that, A one-way valve (7) is provided on the side of the feed tube (504) near the two-stage three-way mixer (505).

8. The yeast nucleotide high-efficiency extraction system according to claim 6, characterized in that, The online near-infrared instrument (6) is used to monitor the content of nucleotides detected online. The online near-infrared instrument (6) is linked with the enzyme supplement module (5) through an external controller.

9. The yeast nucleotide high-efficiency extraction system according to claim 8, characterized in that, The online near-infrared instrument (6) is linked with the electromagnetic flow valve (503) and the enzyme preparation addition pump (502) through an external controller.

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