Integrated embedding device

Abstract

The utility model discloses an integrated embedding device. The integrated embedding device comprises a base and an upper shell, the upper shell comprises a shell body and a fixed film layer, the shell body is provided with a channel penetrating through opposite first and second surfaces thereof, the fixed film layer is connected to the first surface, the fixed film layer covers the channel, the fixed film layer is formed by a plurality of filamentous structures interlaced to form a three-dimensional structure with pores, the pores between at least part of the filamentous structures are communicated, when the upper shell cooperates with the base, the fixed film layer can cooperate with the base to fix the tissue. The utility model can realize that the tissue does not need to be taken out from the embedding box for transfer during embedding, saves embedding time, and the tissue is not easy to be cracked during slicing.

Description

Technical Field

[0001] This application relates to the fields of biological devices and medical devices, and in particular to an integrated embedding device. Background Technology

[0002] Staining paraffin sections of pathological tissue is an important auxiliary method for clinical diagnosis. Paraffin sections are less prone to detachment and can be preserved for a long time after staining, allowing them to be stored long-term, similar to patient medical records. This plays a role in preventing medical accidents and is therefore widely used in major hospitals. In preparing paraffin sections, pathologists place tissue samples taken from the patient into embedding cassettes. Traditional embedding cassettes are made of plastic with multiple small holes to facilitate reagent flow during dehydration. During embedding, the cassette is placed in a dehydrator. After fixation, dehydration, clearing, and paraffin impregnation, the pathologist removes the cassette from the dehydrator and takes the tissue out, placing it on a stainless steel base. Forceps are then used to align and position the tissue, and molten paraffin is added through the embedding machine. After cooling, the tissue is encapsulated in paraffin, forming a paraffin block. Traditional embedding cassettes require removal from the cassette and transfer to different operating platforms, which wastes time and prolongs the tissue processing cycle. In addition, some existing embedding cassettes use membranes to fix tissues, but when tissues are fixed and embedded in these membranes, tissue fragmentation is likely to occur. Utility Model Content

[0003] Therefore, it is necessary to provide an integrated embedding device. The integrated embedding device of this invention enables tissue to be transferred without being removed from the embedding cassette during embedding, and reduces the likelihood of tissue fragmentation during sectioning.

[0004] One embodiment of this application provides an integrated embedding device.

[0005] An integrated embedding device includes a base and an upper shell. The upper shell includes a housing and a fixing membrane layer. The housing has a channel penetrating its opposing first and second surfaces. The fixing membrane layer is connected to the first surface and covers the channel. The fixing membrane layer is a three-dimensional structure with pores formed by multiple interwoven filamentary structures, and at least some of the filamentary structures have interconnected pores. When the upper shell is engaged with the base, the fixing membrane layer can engage with the base to fix tissue.

[0006] In some embodiments, the upper shell further includes a support member connected to the first surface, the support member being closer to the first surface than the fixing film layer, the support member extending at least partially toward the fixing film layer, the support member being used to support and fix the fixing film layer.

[0007] In some embodiments, the support component includes a plurality of support members, wherein at least two of the support members are disposed opposite to each other on both sides of the channel, and the support members on both sides of the channel are respectively close to the connection between the fixed film layer and the first surface, and the support members are used to support the fixed film layer from the edge position of the fixed film layer.

[0008] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0009] (1) The support member and the first surface are rotatably connected by a rotating member, and the support member can be fixed in any one of the rotational positions;

[0010] (2) The material used to prepare the support is a deformable material;

[0011] (3) The support member is capable of elastic deformation.

[0012] In some embodiments, the support portion supports the membrane layer, the support membrane layer covers the channel, and there is a gap between the fixing membrane layer and the support membrane layer. When the upper shell is engaged with the base, the support membrane layer is used to support and fix the fixing membrane layer from the middle position of the fixing membrane layer.

[0013] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0014] (1) The supporting membrane layer is capable of deformation;

[0015] (2) The supporting membrane layer is elastic;

[0016] (3) The supporting membrane layer is porous;

[0017] (4) The supporting membrane layer has an arched structure.

[0018] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0019] (1) The mesh size of the supporting film layer is 100 mesh to 200 mesh;

[0020] (2) The supporting film layer is made of one or two of PET mesh and nylon.

[0021] In some embodiments, the base has a receiving groove for placing tissues. When the upper shell mates with the base, the fixing film layers are all accommodated in the receiving grooves. The fixing film layers can mate with the bottom surface of the receiving grooves to fix the tissues.

[0022] In some embodiments, the supporting membrane layer and the fixing membrane layer are respectively arched in the same direction of curvature, and the maximum height of the fixing membrane layer relative to the first surface is equal to the depth of the receiving groove, or the maximum height of the fixing membrane layer relative to the first surface is 1mm to 10mm greater than the depth of the receiving groove.

[0023] In some embodiments, the opposite sides of the support membrane and / or fixing membrane are respectively connected to the opposite first side edge and second side edge of the channel.

[0024] In some embodiments, the maximum gap between the fixed film layer and the supporting film layer is 1 mm to 2 mm.

[0025] In some embodiments, the integrated embedding device satisfies at least one of the following conditions:

[0026] (1) The housing also has a plurality of through holes penetrating the first surface and the second surface;

[0027] (2) A plurality of fins are provided on the edge of the top surface of the base, wherein two opposite fins extend toward the top of the base and two opposite fins extend toward the bottom of the base;

[0028] (3) The top surface of the base is provided with a limiting groove, the bottom surface of the limiting groove is provided with the receiving groove, and the first surface of the housing can contact and cooperate with the bottom surface of the limiting groove.

[0029] (4) The fixed membrane layer has an arched structure;

[0030] (5) The fixed film layer is a hot melt mesh film;

[0031] (6) The width of the fixed film layer is 1cm to 10cm.

[0032] In some embodiments, the fixed film layer has a multilayer filamentary structure in the thickness direction, with pores in each layer and the pores in each layer being interconnected.

[0033] In some embodiments, the pores of the fixed membrane layer are all interconnected.

[0034] Compared with traditional technologies, the above-mentioned integrated embedding device has at least the following advantages:

[0035] (1) This application is less prone to sectioning. In the embedding process, the tissue is positioned by the fixation film layer. The fixation film layer has multiple three-dimensional structures with pores formed by filamentous structures. The fixation film layer has irregular pore morphology in both the two-dimensional planar structure and the three-dimensional structure. Since the three-dimensional structure is formed by the interweaving of several filamentous structures, the pores between the filamentous structures are interconnected, allowing molten paraffin to penetrate. The paraffin can penetrate into the pores of the fixation film layer. After the paraffin cools, the solidified wax block is continuous rather than discontinuous. Therefore, sectioning of the wax block will basically not produce sectioning.

[0036] (2) This application can significantly save labor and embedding time. This application uses a base and an upper shell that cooperate with each other. The fixed membrane layer can cooperate with the base to fix the tissue. A support component, including a supporting membrane layer or a support member, supports the fixed membrane layer to prevent it from loosening. The supporting membrane layer and / or the support member support the fixed membrane layer. Thus, the supporting membrane layer and / or the support member cooperate with the fixed membrane layer to fix the tissue and maintain its position. In the embedding process, after the above-mentioned integrated embedding device with the fixed tissue is placed in a dehydrator for dehydration, the embedding box is removed from the dehydrator. Melted paraffin can then be directly added through the embedding machine. During this process, there is no need to remove the tissue from the embedding box, transfer it, or reposition it, significantly saving labor and embedding time. Attached Figure Description

[0037] To more clearly illustrate the technical solutions in the embodiments of this application, the accompanying drawings used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0038] To gain a more complete understanding of this application and its beneficial effects, the following description will be provided in conjunction with the accompanying drawings. In the following description, the same reference numerals denote the same parts.

[0039] Figure 1 This is a schematic diagram of the base of the integrated embedding device according to an embodiment of the present invention;

[0040] Figure 2 This is a schematic diagram of the upper shell of the integrated embedding device according to an embodiment of the present invention;

[0041] Figure 3 This is a schematic diagram of the upper shell of the integrated embedding device according to another embodiment of the present invention;

[0042] Figure 4 This is a schematic diagram of the upper shell of the integrated embedding device according to another embodiment of the present invention;

[0043] Figure 5 This is a side view of the upper shell of the integrated embedding device according to an embodiment of the present invention;

[0044] Figure 6 This is a schematic diagram of the base and tissue placement of the integrated embedding device according to an embodiment of the present invention;

[0045] Figure 7 This is a side view of the integrated embedding device and tissue embedding according to an embodiment of the present invention;

[0046] Figure 8 This is a schematic diagram showing the thickness of the wax block that can be sliced ​​according to an embodiment of the present invention;

[0047] Figure 9 This is a physical image of the upper shell according to an embodiment of the present utility model.

[0048] Explanation of reference numerals in the attached figures

[0049] 10. Integrated embedding device; 100. Base; 110. Top surface; 120. Receiving groove; 130. Limiting groove; 140. Fin; 200. Upper shell; 210. Shell; 211. First surface; 212. Second surface; 213. Channel; 214. Support; 215. Through hole; 216. Rotating component; 220. Supporting membrane layer; 230. Fixing membrane layer; 20. Tissue. Detailed Implementation

[0050] To make the above-mentioned objects, features, and advantages of this utility model more apparent and understandable, the specific embodiments of this utility model will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a full understanding of this utility model. However, this utility model can be implemented in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of this utility model. Therefore, this utility model is not limited to the specific embodiments disclosed below.

[0051] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0052] In this utility model, unless otherwise explicitly specified and limited, the terms "installation," "connection," "joining," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise explicitly limited. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0053] In this utility model, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0054] In the description of this utility model, "several" means one or more, "multiple" means two or more, "greater than," "less than," and "exceeding" are understood to exclude the stated number, while "above," "below," and "within" are understood to include the stated number. The use of "first" and "second" in the description is merely for distinguishing technical features and should not be construed as indicating or implying relative importance, or implicitly indicating the number of indicated technical features, or implicitly indicating the order of the indicated technical features.

[0055] In this document, "optionally," "optionally," and "optional" mean that something is optional, that is, it is selected from either "with" or "without." If multiple "options" appear in a technical solution, unless otherwise specified and there are no contradictions or mutual constraints, each "option" is independent. In this application, descriptions such as "optionally contains" and "optionally includes" indicate "contains or does not contain."

[0056] In this application, when numerical intervals (i.e., numerical ranges) are mentioned, unless otherwise specified, the distribution of selectable numerical values ​​within the numerical interval is considered continuous, and includes the two endpoints of the numerical interval (i.e., the minimum and maximum values), as well as every numerical value between these two endpoints. Unless otherwise specified, when a numerical interval refers only to integers within that numerical interval, it includes the two endpoint integers of the numerical range, as well as every integer between the two endpoints, which is equivalent to directly listing every integer. When multiple numerical ranges are provided to describe features or characteristics, these numerical ranges can be merged. In other words, unless otherwise specified, the numerical ranges disclosed in this application should be understood to include any and all subranges included therein. The "numerical value" in the numerical interval can be any quantitative value, such as a number, percentage, ratio, etc. The term "numerical interval" can be broadly included to include percentage intervals, ratio intervals, proportion intervals, etc.

[0057] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0058] This application provides an integrated embedding device to address the problems in the prior art where time intervals exist between steps in the preparation of paraffin sections, resulting in wasted time and extended tissue processing cycles; the traditional paraffin section preparation process requires pathologists to change embedding cassettes and add paraffin to each tissue after dehydration, affecting the stability of the paraffin block quality; and the existing embedding cassettes, which use membrane layers to fix tissues, are prone to sectioning with cracking. The integrated embedding device will be described below with reference to the accompanying drawings.

[0059] The integrated embedding device 10 provided in this application embodiment is exemplary; please refer to [link to example]. Figure 1 and Figure 2 As shown, Figure 1 This is a schematic diagram of the base 100 of the integrated embedding device 10 provided in this application embodiment. Figure 2 This is a schematic diagram of the upper shell 200 of an integrated embedding device 10 according to an embodiment of the present invention. The integrated embedding device 10 of this application can be used for paraffin sectioning, achieving the goals of saving manpower, improving efficiency, and reducing costs during paraffin sectioning. To more clearly illustrate the structure of the integrated embedding device 10, the integrated embedding device 10 will be described below in conjunction with the accompanying drawings.

[0060] For example, please refer to Figure 1 , Figure 2As shown, an integrated embedding device 10 includes a base 100 and an upper shell 200. The upper shell 200 includes a housing 210 and a fixing film layer 230. The housing 210 has a channel 213 penetrating its opposing first surface 211 and second surface 212, allowing the embedded tissue 20 to communicate with the outside. The fixing film layer 230 is connected to the first surface 211. The fixing film layer 230 covers the channel 213. It should be noted that this coverage does not mean that the fixing film layer 230 completely closes the channel 213, but rather that it covers the opening of the channel 213. In this application, when the upper shell 200 is engaged with the base 100, the fixing film layer 230 can engage with the base 100 to fix the tissue 20, wherein the fixing film layer 230 is used to fix the tissue 20.

[0061] In some embodiments, the fixed film layer 230 is a three-dimensional structure with pores formed by the interweaving of multiple filamentous structures. It should be noted that the fixed film layer 230 has multiple pores, which are gaps between several filamentous structures after the filamentous structures are interwoven. These gaps allow molten paraffin to penetrate. When the molten paraffin penetrates the pores and solidifies, it can form a substantially continuous embedding surface on the surface of the tissue 20. The portion where the tissue 20 contacts the filamentous structure of the fixed film layer 230 is a discontinuous surface.

[0062] In some of these embodiments, at least some of the filamentary structures have interconnected pores.

[0063] Preferably, all the pores between the filamentous structures are interconnected.

[0064] In some embodiments, the fixed film layer 230 has a multilayer filamentary structure in the thickness direction, with each layer having pores. See also Figure 9 As shown, Figure 9 The image shows the actual upper shell 200, in which the fixed film layer 230 is composed of multiple filamentous structures distributed in a disorderly manner to form a multi-layered flocculent structure.

[0065] In some embodiments, the pores of the fixed film layer 230 are interconnected in each layer.

[0066] In some embodiments, the pores of each layer of the fixed film 230 are interconnected in the thickness direction. Preferably, all the pores of the fixed film 230 are interconnected.

[0067] In some embodiments, all pores in the fixed film layer 230 are interconnected. It should be noted that the fixed film layer 230 is generally membrane-like, with an internal three-dimensional structure. This three-dimensional structure includes multiple filamentous structures randomly distributed and interwoven, forming pores between these filamentous structures. Therefore, any two-dimensional cross-section of the three-dimensional structure has a mesh-like structure. These pores allow paraffin wax to penetrate, and since all pores are interconnected, paraffin wax can essentially enter all pores as a continuous wax block.

[0068] This application utilizes a base 100 and an upper shell 200 that cooperate with each other, allowing the fixing film layer 230 to engage with the base 100 and thus fix the tissue 20. In the embedding process, after the integrated embedding device 10 with the tissue 20 fixed is placed in a dehydrator for dehydration, the embedding box is removed from the dehydrator, and molten paraffin wax can be directly added through the embedding machine. During this process, there is no need to remove, transfer, or reposition the tissue 20 from the embedding box, significantly saving labor and embedding time. In the embedding process, the fixing film layer 230 has multiple interconnected meshes formed by a filamentous structure, allowing molten paraffin wax to penetrate. After the paraffin wax cools, the solidified wax block is continuous rather than discontinuous; therefore, cracking is minimal when the wax block is sliced.

[0069] In some cases, the fixation membrane may float due to the buoyancy of tissue fluid and / or molten paraffin, causing the fixation membrane to fail to fix the sample tissue. Based on this, in some embodiments, the upper shell 200 also includes a support member connected to the first surface 211. The support member is closer to the first surface 211 than the fixation membrane layer 230, and the support member extends at least partially toward the fixation membrane layer 230. The support member is used to support and fix the fixation membrane layer 230.

[0070] In some embodiments, the support component includes a support member 214, and there are multiple support members 214, wherein at least two support members 214 are disposed opposite to each other on both sides of the channel 213, and the support members 214 on both sides of the channel 213 are respectively close to the connection between the fixed film layer 230 and the first surface 211. The support member 214 is used to support the fixed film layer 230 from the edge position of the fixed film layer 230.

[0071] Preferably, one side of the support member 214 is connected to the edge of the channel 213 on the first surface 211, and the other side of the support member 214 extends toward the fixed film layer 230.

[0072] In some embodiments, the support member 214 may be in the shape of a column, block, plate, etc. The support member 214 only needs to be able to support the fixed film layer 230.

[0073] In some of these embodiments, please refer to Figure 2 , Figure 3 As shown, the support member 214 has a curved structure. Preferably, the curvature of the support member 214 is adapted to the curvature of the fixed film layer 230 in the reset state.

[0074] In some of these embodiments, please refer to Figure 2 , Figure 3 As shown, the length of the support member 214 extending toward the fixed membrane layer 230 can be adjusted as needed.

[0075] In some of these embodiments, please refer to Figure 3 As shown, the support member 214 and the first surface 211 are rotatably connected by a rotating member 216, and the support member 214 can be fixed in any one of the rotational positions.

[0076] In some embodiments, the rotating member 216 may be a hinge structure such as a hinge or hinge joint.

[0077] In some embodiments, the angle between the support member 214 and the fixed film layer 230 can be adjusted as needed. For example, when the fixed film layer 230 is in the reset state, the support member 214 and the fixed film layer 230 are in contact, or when the fixed film layer 230 is in the reset state, there is a certain gap between the support member 214 and the fixed film layer 230. Please refer to [link to relevant documentation]. Figure 2 , Figure 3 As shown, Figure 2 , Figure 3 This shows the contact fit between the support member 214 and the fixed membrane layer 230.

[0078] Preferably, in a specific example, there are two supports 214, which are disposed opposite to each other on both sides of the channel 213. Both supports 214 have a curved structure, and the curvature of the supports 214 is basically the same as the curvature of the fixed film layer 230 in the reset state.

[0079] In some embodiments, the support member 214 is made of a deformable material. For example, the support member 214 may be made of materials such as plastic, silicone, or rubber.

[0080] In some embodiments, the support 214 is capable of elastic deformation.

[0081] More preferably, see Figure 4 As shown, the opening of channel 213 is rectangular, and the two sides along the length of channel 213 form a first side edge and a second side edge, respectively. The support member 214 is connected to the first side edge and the second side edge.

[0082] In some of these embodiments, please refer to Figure 4 , Figure 5 As shown, the support component also includes a support film layer 220. The support film layer 220 and the fixing film layer 230 are respectively connected to the first surface 211. The fixing film layer 230 is located outside the support film layer 220, that is, the support film layer 220 is closer to the first surface 211 than the fixing film layer 230. The support film layer 220 covers the channel 213. In this application, when molten paraffin is added to the base 100 using an embedding machine, the fixing film layer 230, being very light, may float due to the buoyancy of the molten paraffin. In this case, the fixing film layer 230 cannot maintain good contact with and fix the tissue 20. Therefore, this application uses the support film layer 220 positioned at the center of the fixing film layer 230 to support it, preventing it from floating and maintaining contact between the fixing film layer 230 and the tissue 20, thereby improving the embedding effect.

[0083] In some of these embodiments, please refer to Figure 4 , Figure 5 As shown, the support component may include the support member 214 alone or the support membrane layer 220.

[0084] In some embodiments, the support component includes both a support member 214 and a support membrane layer 220. In this case, the two side edges of the support membrane layer 220 are respectively connected to the support member 214 at positions on both sides of the channel 213.

[0085] In some embodiments, the support membrane 220 is capable of deformation.

[0086] In some embodiments, the support membrane 220 is elastic. The support membrane 220 has elastic properties and can undergo a certain degree of deformation, thus supporting the fixation membrane 230 and preventing the fixation membrane 230 and the tissue 20 from loosening.

[0087] In some embodiments, the support membrane 220 is porous. The porous nature of the support membrane 220 has the following advantages: (1) Lightweight: A porous structure usually means that the material contains a large number of voids, so the overall density is low and the weight is light. (2) Good permeability: The pores of the support membrane 220 allow tissue fluid and / or molten paraffin to pass through.

[0088] In some embodiments, the support membrane 220 has an arched structure. The arched structure can effectively support and fix the membrane 230.

[0089] Preferably, in one specific example, the support membrane layer 220 has a porous arched structure.

[0090] In some embodiments, the fixed film layer 230 has an arched structure.

[0091] Preferably, the curvature of the supporting membrane layer 220 is the same as the curvature of the fixed membrane layer 230.

[0092] In some embodiments, the base 100 has a receiving groove 120. In use, the housing 210 covers the base 100 and closes the opening of the receiving groove 120, while the channel 213 allows the receiving groove 120 to communicate with the outside. When the upper housing 200 mates with the base 100, both the supporting membrane layer 220 and the fixing membrane layer 230 are housed within the receiving groove 120. The fixing membrane layer 230 engages with the bottom surface of the receiving groove 120 to fix the tissue 20.

[0093] In some embodiments, the top surface of the base 100 has a receiving groove 120.

[0094] In some of these embodiments, see Figure 4 As shown, the shell 210 has a flat, block-like structure, and the two opposite surfaces of the shell 210 form a first surface 211 and a second surface 212, respectively.

[0095] In some embodiments, a gap exists between the non-end positions of the fixed film layer 230 and the non-end positions of the supporting film layer 220. That is, the ends of the fixed film layer 230 and the ends of the supporting film layer 220 are respectively connected to the first surface. Preferably, see [reference needed]. Figure 4 As shown, the two ends of the fixed film layer 230 correspond to and are attached to the two ends of the supporting film layer 220, respectively.

[0096] In some embodiments, the mesh size of the support film layer 220 is 100 to 200 mesh. For example, the mesh size of the support film layer 220 may be 100 mesh, 110 mesh, 130 mesh, 150 mesh, 170 mesh, 180 mesh, 200 mesh, or other values.

[0097] In some embodiments, the supporting film layer 220 is made of one or both of PET mesh and nylon. The supporting film layer 220 with a PET mesh count of 100-200 mesh can support and fix the film layer 230 on the one hand, and facilitate reagent flow on the other. The PET film layer has the following advantages: (1) High mechanical strength: The PET film layer has high tensile strength and good toughness. Even when thin, it can maintain good mechanical properties and is suitable for applications that require wear resistance and tear resistance. (2) Good transparency: The PET film layer usually has high transparency, with a light transmittance of more than 90%, which is suitable for use as packaging materials, display screen protective films and other applications that require high transparency. (3) Thermal stability: The PET film layer maintains stable physical properties over a wide temperature range and can withstand high temperatures without significant deformation, making it suitable for processing processes that require heat treatment. (4) Chemical stability: The PET film layer has good resistance to most solvents, acids and alkalis and other chemicals, and is not easily corroded, making it suitable for environments that come into contact with chemicals. (5) Environmentally friendly and recyclable: PET is a recyclable material that can be reused after disposal, reducing environmental pollution and meeting the requirements of sustainable development. (6) Cost-effectiveness: Compared with other high-performance plastic films, PET films have lower costs and higher cost-effectiveness, making them suitable for large-scale production and application. (7) Easy to process: PET films can be made into products of different shapes and thicknesses through various processing technologies (such as blow molding, extrusion, coating, etc.), making them highly adaptable.

[0098] Nylon materials possess many excellent physical and chemical properties, including (1) high strength: Nylon fibers are very strong and have good tensile strength. (2) abrasion resistance: Nylon has good resistance to abrasion, so it is often used to manufacture products that require durability. (3) elasticity: Nylon fibers have a certain degree of elasticity and resilience, and can withstand a certain degree of deformation and return to their original shape. Therefore, the nylon material support film layer 220 can improve the support strength of the fixed film layer 230 and improve the positioning effect of the support and tissue 20. (4) chemical resistance: Nylon has good resistance to most chemicals, but strong acids and strong alkalis may damage it. (5) hygroscopicity: Nylon can absorb a certain amount of water and can adsorb excess water during the slicing process. It is easy to understand that in other embodiments, the material used to prepare the support film layer 220 can also be other types of materials.

[0099] Preferably, the material for preparing the support film layer 220 is a PET mesh.

[0100] In some embodiments, the supporting membrane layer 220 and the fixing membrane layer 230 are respectively arched structures with the same bending direction. Preferably, see Figure 5 As shown, both the supporting membrane layer 220 and the fixed membrane layer 230 arch and bend in a direction away from the housing 210.

[0101] In some embodiments, the maximum height of the fixed film layer 230 arching relative to the first surface 211 is equal to the depth of the receiving groove 120.

[0102] In some embodiments, the maximum height of the fixed film layer 230 arching relative to the first surface 211 is greater than or equal to the depth of the receiving groove 120. This arrangement primarily ensures that the fixed film layer 230 and the bottom surface of the receiving groove 120 can clamp and fix the tissue 20.

[0103] In some embodiments, the maximum height of the fixed film layer 230 arching relative to the first surface 211 is greater than the depth of the receiving groove 120 by 1 mm to 10 mm. The maximum height of the fixed film layer 230 arching relative to the first surface 211 can be determined based on the size of the tissue 20 and the depth of the receiving groove 120.

[0104] In some embodiments, the width of the fixing film layer 230 is 1cm to 10cm. The fixing film layer 230 has a certain width to ensure the thickness of the wax block. For example, the width of the fixing film layer 230 can be 1cm, 2cm, 3cm, 5cm, 8cm, 9cm, 10cm, or other values. It should be noted that the length of the fixing film layer 230 refers to the total length between the two ends of the fixing film layer 230 connecting to the first surface 211, and the width of the fixing film layer 230 refers to the length perpendicular to the line connecting the two ends of the fixing film layer 230 to the first surface 211. Figure 4 Width in the X direction (as shown).

[0105] In some embodiments, the opposite sides of the support membrane 220 are respectively connected to the opposite first side edge and the second side edge of the channel 213.

[0106] In some of these embodiments, see Figure 5 As shown, there is a gap between a portion of the fixed membrane layer 230 and the supporting membrane layer 220.

[0107] In some of these embodiments, see Figure 5 As shown, Figure 5This is a side view of the upper shell 200 of the integrated embedding device 10 according to an embodiment of the present invention. The maximum gap between the fixing film layer 230 and the supporting film layer 220 is 1mm to 2mm. A gap exists between the supporting film layer 220 and the fixing film layer 230. The gap between the supporting film layer 220 and the fixing film layer 230 should not be too large or too small. When the gap is too small, the sectioning may easily cut into the supporting film layer 220, causing section breakage; when the gap is too large, the supporting film layer 220 cannot properly support the fixing film layer 230, causing the fixing film layer 230 to deform. In this application, after multiple experimental tests, a gap of 1mm between the supporting film layer 220 and the fixing film layer 230 is found to be most suitable for tissues 20 with a thickness of less than 2mm. It should be noted that, since both ends of the fixing film layer 230 and the supporting film layer 220 are connected to the first surface, the maximum gap between the fixing film layer 230 and the supporting film layer 220 refers to the distance between the middle position of the arched fixing film layer 230 and the middle position of the arched supporting film layer 220. Furthermore, the thickness of the tissue 20 refers to... Figure 8 The distance between the up and down arrows in the angle shown.

[0108] In some embodiments, the fixing film 230 is a hot-melt mesh. Using a hot-melt mesh for the fixing film 230 allows for lightweight and easily deformable properties, effectively maintaining the position of the tissue 20 and preventing sectioning during paraffin block cutting.

[0109] In some embodiments, the hot melt web is formed by spinnereting.

[0110] In some embodiments, the hot melt mesh includes several filamentous structures, which are interwoven to form a three-dimensional structure. The three-dimensional structure has multiple pores, so that the hot melt mesh has mesh holes in any two-dimensional cross-section and in the three-dimensional structure.

[0111] It's important to note that hot melt abrasive is a special type of hot melt adhesive product, typically exhibiting a mesh structure and resembling nonwoven fabric in appearance. Unlike ordinary abrasives, hot melt abrasives are produced through specialized equipment that spins and weaves the fibers, resulting in a mesh structure with multiple interwoven filaments. This makes hot melt abrasives very lightweight, suitable for tissue localization. In contrast, traditional abrasives have a regular, continuous structure, roughly resembling a grid of holes, and their solid portion is tightly packed. Therefore, they cannot allow for continuous paraffin infiltration. When used for sectioning, the paraffin filling of ordinary abrasives is divided into multiple independent units, causing discontinuity in the paraffin. The paraffin in both the two-dimensional cross-section and the three-dimensional structure of ordinary abrasives is discontinuous, which can lead to sectioning fragmentation. Based on this, in this application, the hot-melt mesh formed by spinnereting has a three-dimensional structure consisting of randomly interwoven filaments. Therefore, the mesh distribution of the hot-melt mesh on a single two-dimensional cross-section is disordered. Thus, after being filled with paraffin wax, it can penetrate into the pores between multiple filaments. Since the pores formed by the filaments are basically interconnected, the paraffin wax solidifies into a continuous wax block, and subsequent slicing will not result in cracking.

[0112] In some embodiments, the hot melt web film can be purchased from Suzhou Huiyang Adhesive Products Co., Ltd., model: W6165TDS.

[0113] In some of these embodiments, see Figure 4 As shown, the housing 210 also has several through holes 215 penetrating the first surface 211 and the second surface 212. The housing 210 is provided with multiple through holes 215 to facilitate the flow of reagents.

[0114] In some of these embodiments, see Figure 4 As shown, multiple through holes 215 on the housing 210 are distributed on both sides of the channel 213. It is easy to understand that, in one specific example, the multiple through holes 215 on the housing 210 can also be distributed on all four sides of the channel 213. In this application, providing multiple through holes 215 on the housing 210 enables the flow of reagents between the first surface 211 and the second surface 212 on the housing 210, facilitating slicing operations and reducing the frequent disassembly and assembly of the housing 210 and the base 100.

[0115] In some of these embodiments, please refer again. Figure 1As shown, the top surface 110 of the base 100 is provided with a limiting groove 130. The bottom surface of the limiting groove 130 is provided with a receiving groove 120. During assembly, the first surface 211 of the housing 210 can contact and engage with the bottom surface of the limiting groove 130. At this time, the support part 214 extends into the receiving groove 120, and both the supporting film layer 220 and the fixing film layer 230 are completely located within the receiving groove 120. This arrangement can improve the compactness of the assembly and improve the slicing effect.

[0116] In some embodiments, a plurality of fins 140 are provided on the edge of the top surface 110 of the base 100. Two opposing fins 140 extend toward the top of the base 100. After the base 100 is provided with the receiving groove 120, the limiting groove 130, and the plurality of fins 140, see [reference needed]. Figure 1 As shown, the base 100 has a box-like structure. In this application, several fins are provided on the edge of the top surface 110 of the base 100, and the fins 140 at different positions can improve the stability of the base 100, prevent the base 100 from tipping over during the slicing process, and improve the slicing stability.

[0117] In some embodiments, the base 100 is made of stainless steel. Since stainless steel and paraffin have different shrinkage rates, using stainless steel for the base 100 facilitates the removal of the tissue 20 from the paraffin block after embedding and helps to fix the membrane layer 230, thus improving sectioning efficiency.

[0118] The aforementioned integrated embedding device 10, when in use, specifically includes the following steps:

[0119] (1) See Figure 6 As shown, Figure 6 This is a schematic diagram of the placement of the base 100 and tissue 20 in an integrated embedding device 10 according to an embodiment of the present invention. The tissue 20 is placed in the middle of the bottom surface of the receiving groove 120 of the base 100.

[0120] (2) Invert the upper shell 200, fix the membrane layer 230 to cover and seal the opening of the receiving groove 120, and bring the fixation membrane layer 230 into contact with the tissue 20. The fixation membrane layer 230 fixes the tissue 20. See [reference] Figure 7 As shown, Figure 7This is a side view of the integrated embedding device 10 of this utility model during tissue embedding according to an embodiment of the present invention. The fixing membrane layer 230 is deformed by the constraint of the tissue 20. The deformed fixing membrane layer 230 positions the tissue 20. Since there is a gap between the supporting membrane layer 220 and the fixing membrane layer 230, there is no contact between the supporting membrane layer 220 and the tissue 20. Alternatively, the support member 214 is in contact with the edge of the fixing membrane layer 230, but there is no contact between it and the tissue 20. After the upper shell 200 and the base 100 are assembled, they are fixed and kept in a fixed state throughout the entire paraffin embedding process. After the integrated embedding device 10 with the tissue 20 is placed in a dehydrator for dehydration, the embedding box is removed from the dehydrator.

[0121] (3) Molten paraffin is injected into the receiving groove 120 of the base 100 through the through hole 215 by the embedding machine. The supporting film layer 220 supports the fixing film layer 230, and / or the supporting member 214 supports the edge position of the fixing film layer 230. The fixing film layer 230 remains in contact with the tissue 20. After the paraffin cools down, the tissue 20 is embedded.

[0122] (4) Remove the upper shell 200. As the upper shell 200 moves, the fixation film 230 gradually detaches from the base 100. The paraffin, tissue 20, and part of the fixation film 230 form an embedded wax block. Since the solidified paraffin is continuous rather than discontinuous within the mesh of the fixation film 230, when the embedded wax block is sectioned, the resulting sections contain only paraffin, filamentous parts of the fixation film 230, and tissue 20, without other soft materials. Therefore, section cracking can be avoided, and the resulting sections are basically free of cracking. See Figure 8 As shown, Figure 8 This is a schematic diagram showing the thickness of the wax block that can be sliced ​​according to an embodiment of the present invention. Figure 8 The area indicated by the two arrows above and below represents the thickness of the wax block that can be sliced.

[0123] In summary, this application minimizes the occurrence of sectioning cracks. During the embedding process, the tissue 20 is positioned by the fixation film 230, which has multiple three-dimensional structures with pores formed by filamentous structures. The fixation film 230 exhibits irregular pore morphology in both its two-dimensional planar and three-dimensional structures. Since the three-dimensional structure is formed by the interweaving of several filamentous structures, the pores between the filamentous structures are interconnected, allowing molten paraffin to penetrate. The paraffin can penetrate into each gap of the fixation film 230. After the paraffin cools, the solidified wax block is continuous rather than discontinuous. Therefore, sectioning of the wax block is virtually free of cracks.

[0124] The integrated embedding device 10 of this application can significantly save labor and embedding time. This application uses a base 100 and an upper shell 200 to cooperate with each other. The fixing film layer 230 can cooperate with the base 100 to fix the tissue 20. Since there is a certain gap between the supporting film layer 220 and the fixing film layer 230, in the initial stage of contacting the tissue 20, the fixing film layer 230 contacts the tissue 20 first and fixes the tissue 20. Then, the supporting film layer 220 supports the fixing film layer 230 to prevent the fixing film layer 230 from loosening. The supporting film layer 220 supports the fixing film layer 230. In this way, the supporting film layer 220 and the fixing film layer 230 cooperate to fix the tissue 20 and keep the tissue 20 in position; and / or, the supporting member 214 supports the fixing film layer 230 from the edge position. The supporting member 214 provides a certain supporting force to the fixing film layer 230 to keep the fixing film layer 230 fixed and positioned to the tissue 20. In the embedding process, after the integrated embedding device 10 with the tissue 20 fixed thereon is placed in a dehydrator for dehydration, the embedding box is taken out of the dehydrator and molten paraffin is directly added through the embedding machine. During this process, there is no need to take the tissue 20 out of the embedding box for transfer and repositioning, which greatly saves labor and embedding time.

[0125] In the above embodiments, the descriptions of each embodiment have different focuses. For parts not described in detail in a certain embodiment, please refer to the relevant descriptions in other embodiments.

[0126] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0127] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of this utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. An integrated embedding device, characterized in that, The device includes a base and an upper shell. The upper shell includes a housing and a fixing membrane layer. The housing has a channel penetrating its opposing first and second surfaces. The fixing membrane layer is connected to the first surface and covers the channel. The fixing membrane layer is formed by multiple interwoven filamentary structures to create a porous three-dimensional structure. At least some of the pores are interconnected. When the upper shell is engaged with the base, the fixing membrane layer can engage with the base to fix the tissue.

2. The integrated embedding device according to claim 1, characterized in that, The upper shell also includes a support member connected to the first surface. The support member is closer to the first surface than the fixed film layer and extends at least partially toward the fixed film layer. The support member is used to support and fix the fixed film layer.

3. The integrated embedding device according to claim 2, characterized in that, The supporting component includes a plurality of support members, wherein at least two of the support members are disposed opposite to each other on both sides of the channel, and the support members on both sides of the channel are respectively close to the connection between the fixed film layer and the first surface. The support members are used to support the fixed film layer from the edge position of the fixed film layer.

4. The integrated embedding device according to claim 3, characterized in that, The integrated embedding device satisfies at least one of the following conditions: (1) The support member and the first surface are rotatably connected by a rotating member, and the support member can be fixed in any one of the rotational positions; (2) The support member is capable of elastic deformation.

5. The integrated embedding device according to claim 2, characterized in that, The supporting component includes a supporting membrane layer that covers the channel. There is a gap between the fixing membrane layer and the supporting membrane layer. When the upper shell is engaged with the base, the supporting membrane layer is used to support and fix the fixing membrane layer from the middle position of the fixing membrane layer.

6. The integrated embedding device according to claim 5, characterized in that, The integrated embedding device satisfies at least one of the following conditions: (1) The supporting membrane layer is capable of deformation; (2) The supporting membrane layer is elastic; (3) The supporting membrane layer is porous; (4) The supporting membrane layer has an arched structure; (5) The mesh size of the supporting membrane is 100-200 mesh.

7. The integrated embedding device according to any one of claims 5 to 6, characterized in that, The base has a receiving groove for placing tissues. When the upper shell is engaged with the base, the fixing film layers are all accommodated in the receiving groove. The fixing film layers can engage with the bottom surface of the receiving groove to fix the tissues.

8. The integrated embedding device according to claim 7, characterized in that, The supporting membrane layer and the fixing membrane layer are respectively arched structures with the same bending direction, and the maximum height of the fixing membrane layer relative to the first surface is equal to the depth of the receiving groove, or the maximum height of the fixing membrane layer relative to the first surface is 1mm to 10mm greater than the depth of the receiving groove.

9. The integrated embedding device according to any one of claims 5-6 and 8, characterized in that, The integrated embedding device satisfies at least one of the following conditions: (1) The two sides of the supporting membrane layer and / or the fixing membrane layer are respectively connected to the first side edge and the second side edge of the channel; (2) The maximum gap between the fixed film layer and the supporting film layer is 1mm~2mm.

10. The integrated embedding device according to any one of claims 1 to 5 and 8, characterized in that, The integrated embedding device satisfies at least one of the following conditions: (1) The housing also has a plurality of through holes penetrating the first surface and the second surface; (2) A plurality of fins are provided on the edge of the top surface of the base, wherein two opposite fins extend toward the top of the base and two opposite fins extend toward the bottom of the base; (3) The fixed membrane layer has an arched structure; (4) The fixed film layer is a hot melt mesh film; (5) The width of the fixed film layer is 1cm to 10cm; (6) The fixed film layer has a multi-layer filamentary structure in the thickness direction, and each layer has pores, and the pores in each layer are interconnected; (7) The pores of the fixed membrane layer are all interconnected.

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