A bacterial endotoxin gel method detection device based on automation control
The automated bacterial endotoxin gel electrophoresis detection device solves the problems of cumbersome detection process and human interference, and achieves efficient and reliable detection results and data traceability.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- TIANDA TIANFA TECH
- Filing Date
- 2025-06-06
- Publication Date
- 2026-05-29
AI Technical Summary
Existing bacterial endotoxin gel electrophoresis methods are cumbersome, manual testing is inefficient and easily subject to human interference, and test results are difficult to preserve and trace.
Design an automated control-based bacterial endotoxin gel electrophoresis detection device, comprising a sample rack, dilution rack, shaking rack, horseshoe crab reagent area, constant temperature area and camera assembly, to achieve automated dilution, shaking, sample addition, detection and result analysis.
It improves detection efficiency, reduces the risk of human interference, enables automatic result determination and data traceability, and is suitable for large-scale sample testing.
Smart Images
Figure CN224303692U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of bacterial endotoxin detection technology, and in particular to a bacterial endotoxin gel method detection device based on automated control. Background Technology
[0002] Bacterial endotoxins are lipopolysaccharides (LPS) found in the cell walls of Gram-negative bacteria. They are primarily composed of lipid A, core polysaccharides, and O-antigen, and are released during bacterial death or reproduction. They possess strong pyrogenicity and biological activity; even trace amounts can induce fever, shock, and even organ failure in humans. Therefore, they are contaminants that must be strictly controlled in pharmaceuticals, medical devices, and biological products. The gel electrophoresis method is based on the coagulation reaction principle of Limulus amebocyte lysate (LAL) reagent: endotoxins activate an enzyme cascade reaction in LAL cells, ultimately forming a gel-like solid. This method involves serially diluting samples, mixing them with LAL reagent, incubating at 37°C for 1 hour, and then observing the gel formation to determine if the endotoxin content exceeds the limit.
[0003] Bacterial endotoxin testing is a crucial quality indicator for ensuring the safety of injectable drugs. Traditional bacterial endotoxin gel electrophoresis typically relies on manual testing, using a Limulus amebocyte lysate (LAL) reagent enzymatic reaction to obtain the final test results. The drawbacks of existing testing methods are:
[0004] 1. The testing process is cumbersome, and manual testing is inefficient;
[0005] 2. The risk of human interference during the experiment is high, which can easily interfere with the determination of drug results;
[0006] 3. Test results are difficult to preserve, test data cannot be traced, and manual evaluation cannot be fully trusted;
[0007] To address the challenges of large-scale endotoxin gel electrophoresis experiments and save manpower, a fully automated dilution and detection device for bacterial endotoxin gel electrophoresis was designed. Utility Model Content
[0008] The purpose of this invention is to provide an automated control-based bacterial endotoxin gel assay detection device, which solves the problems of existing detection methods being cumbersome, inefficient, and prone to human interference, easily interfering with the determination of drug results.
[0009] To achieve the above objectives, an automated control-based bacterial endotoxin gel electrophoresis detection device is provided, comprising a worktable, a sample rack at one end of the upper part of the worktable, a dilution rack at the right end of the sample rack, three sets of dilution racks, a shaking rack at the right end of the dilution rack, and a tip head area installed at the rear end of the shaking rack.
[0010] The sample holder and the oscillation rack are fixedly connected to two supports at their rear ends. A three-axis sliding frame is fixedly connected to the supports, and a pipette is mounted on the three-axis sliding frame.
[0011] According to the aforementioned automated control-based bacterial endotoxin gel electrophoresis detection device, a horseshoe crab reagent area is provided on one side of the shaking rack.
[0012] According to the aforementioned automated control-based bacterial endotoxin gel electrophoresis detection device, a hopper is provided on the other side of the horseshoe crab reagent area.
[0013] According to the aforementioned automated control-based bacterial endotoxin gel electrophoresis detection device, a constant temperature zone is correspondingly provided on the other side of the hopper.
[0014] According to the aforementioned automated control-based bacterial endotoxin gel method detection device, a camera assembly is correspondingly provided at the other end of the constant temperature zone. The camera assembly includes a camera bracket and a camera mounted on the camera bracket.
[0015] According to the aforementioned automated control-based bacterial endotoxin gel electrophoresis detection device, two sets of belt drive assemblies are installed at the rear end of the constant temperature zone, and mounting brackets and flipping components are respectively installed on the belt drive assemblies.
[0016] According to the aforementioned automated control-based bacterial endotoxin gel electrophoresis detection device, the mounting frame is equipped with grippers.
[0017] The above-mentioned solution has the following beneficial effects:
[0018] 1. This patented device is easy to operate, has a user-friendly interface, reduces labor costs, and improves work efficiency. The device is highly automated, minimizing the risk factors that may be caused by human interference. At the same time, the entire device can automatically determine the gelation results, calculate the results, and monitor the entire test process in real time. It also enables the traceability of metadata and can simultaneously perform endotoxin gelation detection on a large number of samples, meeting the needs of large-scale testing.
[0019] Additional aspects and advantages of this invention will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the invention. Attached Figure Description
[0020] The present invention will be further described below with reference to the accompanying drawings and embodiments;
[0021] Figure 1 This is a front view of a bacterial endotoxin gel electrophoresis detection device based on automated control according to this utility model;
[0022] Figure 2This is a rear view of a bacterial endotoxin gel electrophoresis detection device based on automated control according to this utility model.
[0023] Figure 3 This is a top view of a bacterial endotoxin gel assay detection device based on automated control, according to this utility model.
[0024] Legend:
[0025] 1. Worktable; 2. Tilting assembly; 3. Grippers; 4. Tip head area; 5. Triaxial sliding frame; 6. Sample rack; 7. Dilution rack; 8. Shaking rack; 9. Limulus amebocyte lysate (LAL) reagent area; 10. Storage hopper; 11. Temperature control zone; 12. Camera assembly; 13. Pipette; 14. Mounting frame; 15. Support. Detailed Implementation
[0026] This section will describe in detail the specific embodiments of the present utility model. The preferred embodiments of the present utility model are shown in the accompanying drawings. The purpose of the drawings is to supplement the textual description with graphics, so that people can intuitively and vividly understand each technical feature and overall technical solution of the present utility model, but they should not be construed as limiting the scope of protection of the present utility model.
[0027] Reference Figure 1-3 This utility model provides an automated control-based bacterial endotoxin gel electrophoresis detection device, which includes a workbench 1. A sample rack 6 is provided at one end of the upper part of the workbench 1. The sample rack 6 has multiple sample slots inside, which can store reagents and improve their use. A dilution rack 7 is provided at the right end of the sample rack 6. There are three sets of dilution racks 7. A shaking rack 8 is provided at the right end of the dilution rack 7. A tip head area 4 is installed at the rear end of the shaking rack 8.
[0028] The sample holder 6 and the rear end of the shaking rack 8 are fixedly connected to two supports 15. A triaxial sliding frame 5 is fixedly connected to the support 15. A pipette 13 is installed on the triaxial sliding frame 5. A horseshoe crab reagent area 9 is set on one side of the shaking rack 8. After the consumables are placed, the pipette 13 completes the dilution and sample addition work through the program instructions to complete the gradient dilution and ensure that the concentration meets the detection requirements. After diluting the horseshoe crab reagent placed in the shaking module, the pipette 13 will start shaking to ensure that it is mixed evenly. The pipette 13 carries a disposable pyrogen-free tip to draw up the shaken mixture and accurately add it to the gel method detection tube or microplate to avoid cross-contamination.
[0029] On the other side of the horseshoe crab reagent area 9, there is a material hopper 10. On the other side of the material hopper 10, there is a constant temperature zone 11. At the other end of the constant temperature zone 11, there is a camera assembly 12. The camera assembly 12 includes a camera bracket and a camera mounted on the camera bracket. Two sets of belt drive assemblies are installed at the rear end of the constant temperature zone 11. A mounting frame 14 and a flipping assembly 2 are respectively installed on the belt drive assemblies. A gripper 3 is installed on the mounting frame 14. After dilution, the gripper 3 first picks up the automatically replenished fixture stored in the material hopper 10 and makes the fixture hold the diluted sample. The gripper 3 then places the fixture with the test tube into the constant temperature component. After the experiment is over, the sample is flipped. The rotating component 2 grips the test tube with clamps, moves it to a fixed position, flips it, and uses a camera to photograph and analyze the experimental results, which are then stored. The gripper 3 and the flipping component are both driven by belt transmission, and the bottoms of the gripper 3 and the flipping component slide on slide rails to ensure the stability of the entire moving component during movement. The flipping component integrates a motor to achieve the flipping effect. In actual use, the entire workbench 1 is installed inside a cabinet, which contains a fan, a display screen, and an alarm light. The fan provides clean air, the display screen assists the experimenter in operation, and the alarm light alerts the experimenter in case of material shortages or other issues.
[0030] Working principle: The dilution and sample loading section consists of the following components: an XYZ triaxial pipette equipped with a quantitative dispensing tip, a sample area, a dilution area, a shaking area, and a horseshoe crab reagent area. The specific operation in the dilution and sample loading area is as follows: the experimenter places various sample tubes, dilution tubes, standard tubes, and horseshoe crab reagent tubes into their respective devices. The XYZ triaxial pipette then performs the specific liquid aspiration, loading, and dispensing, completing the dilution process as designed. After the dilution process is complete, the diluted product is placed in the horseshoe crab reagent area. In zone 9, the subsequent clamping operation is performed by the constant temperature zone 11 detection section. The constant temperature detection section includes a clamping component, a hopper component, a flipping component, a camera component, and a constant temperature component. The specific operation is as follows: after dilution is completed, the clamping jaw 3 first clamps the automatically replenished fixture stored in the hopper 10 and clamps the diluted sample. The clamping jaw 3 then places the fixture with the test tube into the constant temperature component. After the experiment time ends, the flipping component 2 clamps the test tube with the fixture, moves it to a fixed position, flips it, and the camera takes pictures to analyze the experimental results and save them.
[0031] The embodiments of the present utility model have been described in detail above with reference to the accompanying drawings. However, the present utility model is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present utility model.
Claims
1. An automated control-based bacterial endotoxin gel electrophoresis detection device, comprising a workbench (1), characterized in that, A sample rack (6) is provided at one end of the upper part of the workbench (1), and a dilution rack (7) is provided at the right end of the sample rack (6). There are three sets of dilution racks (7). A shaking rack (8) is provided at the right end of the dilution rack (7). A tip head area (4) is installed at the rear end of the shaking rack (8). The sample holder (6) and the oscillation rack (8) are fixedly connected to two supports (15) at their rear ends. A three-axis sliding frame (5) is fixedly connected to the support (15), and a pipette (13) is installed on the three-axis sliding frame (5).
2. The bacterial endotoxin gel electrophoresis detection device based on automated control according to claim 1, characterized in that, A horseshoe crab reagent area (9) is provided on one side of the oscillator (8).
3. The bacterial endotoxin gel electrophoresis detection device based on automated control according to claim 2, characterized in that, A hopper (10) is provided on the other side of the horseshoe crab reagent area (9).
4. The bacterial endotoxin gel electrophoresis detection device based on automated control according to claim 3, characterized in that, A constant temperature zone (11) is provided on the other side of the silo (10).
5. The bacterial endotoxin gel electrophoresis detection device based on automated control according to claim 4, characterized in that, At the other end of the constant temperature zone (11), a camera assembly (12) is provided, which includes a camera bracket and a camera mounted on the camera bracket.
6. The bacterial endotoxin gel electrophoresis detection device based on automated control according to claim 4, characterized in that, Two sets of belt drive assemblies are installed at the rear end of the constant temperature zone (11), and mounting brackets (14) and flipping components (2) are respectively installed on the belt drive assemblies.
7. The bacterial endotoxin gel electrophoresis detection device based on automated control according to claim 6, characterized in that, The mounting bracket (14) is equipped with grippers (3).