Animal liver tissue rapid cryopreservation sampler
By designing a collaborative structure of rotating ring, connecting column, pawl, ratchet, threaded column, cylinder, and push plate, the problem of tight adhesion between the embedding adhesive and the inner wall was solved, enabling smooth extraction of the sample and the embedding adhesive, reducing operation time, avoiding sample damage, and improving the practicality of the sampler.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- SHENZHEN TRADITIONAL CHINESE MEDICINE HOSPITAL
- Filing Date
- 2025-09-02
- Publication Date
- 2026-06-02
AI Technical Summary
Existing rapid cryosamplers for animal liver tissue are difficult to remove because the embedded gel adheres tightly to the inner wall after freezing, and lacks a convenient ejection mechanism. This results in the sample and embedded gel being difficult to remove, which is time-consuming and may damage the sample.
A structure including a rotating ring, a connecting post, a pawl, a ratchet and a threaded post, a cylinder, and a push plate is designed. By rotating the rotating ring, the connecting post and the pawl rotate synchronously. The pawl pushes the ratchet to rotate, which drives the threaded post to rotate. The cylinder and the threaded post are threadedly connected. The slide rod is guided in the slide groove. The cylinder moves upward stably, pushing the rubber plug and the push plate to push out the embedded glue.
This method enables the successful extraction of samples and embedding gels, shortens operation time, avoids sample damage, and improves sampling efficiency and practicality.
Smart Images
Figure CN224317331U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of animal liver tissue sampling technology, and in particular to a rapid cryogenic sampler for animal liver tissue. Background Technology
[0002] The animal liver tissue rapid cryosampler is a device specifically designed for sampling animal liver tissue. It can quickly sample animal liver tissue and has a freezing function, which can rapidly freeze the sample during or after sampling to maintain the bioactivity and original state of the liver tissue, reducing the risk of sample deterioration or degradation at room temperature. It may employ a convenient sampling structure, such as a specially designed sampling probe, combined with efficient freezing components, such as a built-in cooling module, which can quickly reduce the temperature of the sampling area and the sample, ensuring the timeliness of sampling and the quality of the sample. It is widely used in biomedical research, veterinary testing, and other fields, providing reliable liver tissue samples for related experiments and analyses.
[0003] Existing rapid cryosamplers for animal liver tissue often fail to remove the sample and embedding gel smoothly after freezing, due to the tight adhesion between the gel and the inner wall of the sampler and the lack of a convenient ejection mechanism. This not only increases the operation time but may also damage the sample if it is forcibly removed.
[0004] Therefore, the existing rapid cryosamplers for animal liver tissue have problems such as the sample being tightly adhered to the inner wall after freezing due to the embedding gel, and the lack of a convenient ejection mechanism, making it difficult to remove the sample and embedding gel. This is time-consuming and may damage the sample if forcibly removed. There is an urgent need to design a new type of rapid cryosampler for animal liver tissue. Utility Model Content
[0005] To overcome the problems of existing animal liver tissue rapid cryosamplers, which are difficult to remove because the embedded gel adheres tightly to the inner wall after freezing and lacks a convenient ejection mechanism, resulting in time-consuming extraction and potential sample damage if forcibly removed.
[0006] The technical solution of this utility model is as follows: a rapid cryogenic sampler for animal liver tissue, comprising an auxiliary cylinder and a circular frame installed on the top of the auxiliary cylinder. A push hole is provided at the bottom of the circular frame, and a push plate is provided inside the push hole. A rubber stopper is connected to the bottom of the push plate, and a cylinder is connected to the bottom of the rubber stopper. A threaded column is threadedly connected inside the cylinder. Sliding grooves are provided on the left and right sides inside the auxiliary cylinder. Sliding rods are connected to the left and right ends of the cylinder and are slidably connected to the sliding grooves. A ratchet is connected to the bottom of the threaded column at the bottom of the auxiliary cylinder. The ratchet is rotatably connected to the auxiliary cylinder. A rotating ring is rotatably connected to the outer end of the auxiliary cylinder. A connecting post is connected to the bottom of the rotating ring. A pawl is rotatably connected to the bottom of the connecting post through a return shaft. The pawl is movably connected to the ratchet. The rotation of the rotating ring drives the connecting post and the pawl to rotate. The pawl pushes the ratchet to rotate at the bottom of the auxiliary cylinder. The rotation of the ratchet drives the sliding rod. The rotation of the sliding rod pushes the cylinder upward. The cylinder pushes the sliding rod to slide in the sliding groove. The upward movement of the cylinder pushes the rubber stopper and pushes the push plate out.
[0007] Preferably, by setting up a rotating ring, connecting post, pawl, ratchet, threaded post, cylinder, and push plate in conjunction with the threaded post, cylinder, and push plate, the sample and embedding adhesive can be smoothly removed. When the rotating ring rotates, it drives the connecting post and pawl to rotate synchronously. The pawl, with the help of the return shaft, makes close contact with the ratchet and pushes it to rotate. The rotation of the ratchet drives the threaded post to rotate. Since the cylinder and the threaded post are threadedly connected, and the sliding rods on both sides of the cylinder slide in the groove to guide it, the rotation of the threaded post will drive the cylinder to move steadily upward, thereby pushing the rubber stopper and push plate to move upward. The push plate pushes out the embedding adhesive, which effectively solves the problem of the adhesive sticking tightly to the inner wall of the sampler, greatly reduces the operation time, and avoids sample damage caused by forced removal.
[0008] Preferably, a freezing frame is connected to the upper outer end of the auxiliary cylinder, and support blocks are connected to the left and right ends of the auxiliary cylinder.
[0009] Preferably, a chassis is connected between the two support blocks, and a fixing block is connected to the upper left side of the auxiliary cylinder.
[0010] Preferably, a rotating column is rotatably connected to the top of the fixed block, and a blade is connected to the right end of the rotating column.
[0011] Preferably, a handle is connected to the left end of the rotating column, and an anti-slip ring is connected to the bottom of the circular frame.
[0012] Preferably, pushing the handle causes the rotating column to rotate within the fixed block, and the rotation of the rotating column causes the blade to rotate.
[0013] Preferably, the anti-slip ring is used to control the adhesive tension, and the support block and chassis are used to support the auxiliary cylinder.
[0014] The beneficial effects of this utility model are:
[0015] 1. By setting up a rotating ring, connecting post, pawl, ratchet, threaded post, cylinder, and push plate in coordination, the sample and embedding adhesive can be smoothly removed. Rotating the rotating ring causes the connecting post and pawl to rotate synchronously. The pawl, with the help of the return shaft, pushes the ratchet to rotate, which in turn drives the threaded post to rotate. Because the cylinder and the threaded post are threadedly connected, and the slide rod is guided by the slide groove, the cylinder moves smoothly upward, pushing the rubber stopper and push plate to eject the embedding adhesive. This can shorten the operation time, improve efficiency, avoid sample damage to ensure integrity, provide a reliable basis for subsequent testing, and improve practicality. Attached Figure Description
[0016] Figure 1 The diagram shown is a three-dimensional structural schematic of the animal liver tissue rapid cryopreservation sampler of this utility model.
[0017] Figure 2 The diagram shown is a three-dimensional bottom view of the animal liver tissue rapid cryopreservation sampler of this utility model.
[0018] Figure 3 The diagram shown is a three-dimensional side sectional view of the animal liver tissue rapid cryopreservation sampler of this utility model.
[0019] Figure 4 The diagram shown is a three-dimensional top-down view of the animal liver tissue rapid cryogenic sampler of this utility model.
[0020] Figure 5 This invention relates to a rapid cryogenic sampling device for animal liver tissue. Figure 3 Enlarged structural diagram of point A in the middle.
[0021] Explanation of reference numerals in the attached drawings: 1. Auxiliary cylinder; 21. Circular frame; 22. Push hole; 23. Push plate; 24. Rubber plug; 25. Cylinder; 26. Threaded post; 27. Slide groove; 28. Slide rod; 29. Ratchet; 210. Rotary ring; 211. Connecting post; 212. Pawl; 31. Freezing frame; 32. Support block; 33. Base; 34. Fixing block; 35. Rotary post; 36. Blade plate; 37. Handle; 38. Anti-slip ring. Detailed Implementation
[0022] The present invention will be further described below with reference to the accompanying drawings and embodiments.
[0023] Please see Figures 1-5This utility model provides an embodiment of a rapid cryogenic sampling device for animal liver tissue, comprising an auxiliary cylinder 1 and a circular frame 21 mounted on top of the auxiliary cylinder 1. A push hole 22 is provided at the bottom of the circular frame 21, and a push plate 23 is disposed inside the push hole 22. A rubber stopper 24 is connected to the bottom of the push plate 23, and a cylinder 25 is connected to the bottom of the rubber stopper 24. A threaded post 26 is threadedly connected inside the cylinder 25. Sliding grooves 27 are provided on the left and right sides inside the auxiliary cylinder 1, and sliding rods 28 are connected to the left and right ends of the cylinder 25. The threaded post 26 is slidably connected to the groove 27. A ratchet 29 is connected to the bottom of the auxiliary cylinder 1, extending from the bottom of the threaded post 26. The ratchet 29 is rotatably connected to the auxiliary cylinder 1. A rotating ring 210 is rotatably connected to the outer end of the auxiliary cylinder 1. A connecting post 211 is connected to the bottom of the rotating ring 210. A pawl 212 is rotatably connected to the bottom of the connecting post 211 via a return shaft. The pawl 212 is movably connected to the ratchet 29. Rotation of the rotating ring 210 drives the connecting post 211 and the pawl 212 to rotate, and the pawl 212 pushes the ratchet 29 to rotate at the bottom of the auxiliary cylinder 1. The ratchet 29 rotates, driving the slide bar 28. The slide bar 28 rotates, pushing the cylinder 25 upward. The cylinder 25 pushes the slide bar 28 to slide within the groove 27. The upward movement of the cylinder 25 pushes the rubber plug 24, causing the push plate 23 to be ejected. By setting up the rotating ring 210, connecting post 211, pawl 212, ratchet 29, threaded post 26, cylinder 25, and push plate 23, the sample and embedding adhesive can be smoothly removed. When the rotating ring 210 rotates, it drives the connecting post 211 and pawl 212 to rotate synchronously. The pawl 212 uses a return shaft to rotate. The force exerted by the ratchet 29 is in close contact with the ratchet 29 and pushes it to rotate. The rotation of the ratchet 29 drives the threaded column 26 to rotate. Since the cylinder 25 is threadedly connected to the threaded column 26, and the slide rods 28 on both sides of the cylinder 25 slide in the slide groove 27 to play a guiding role, the rotation of the threaded column 26 will drive the cylinder 25 to move upward steadily, thereby pushing the rubber stopper 24 and the push plate 23 to move upward. The push plate 23 pushes out the embedding adhesive, which effectively solves the problem of the adhesive adhering tightly to the inner wall of the sampler, greatly reduces the operation time, and avoids sample damage caused by forced removal.
[0024] Please see Figures 1-5In this embodiment, a freezing frame 31 is connected to the upper outer side of the auxiliary cylinder 1, support blocks 32 are connected to the left and right ends of the auxiliary cylinder 1, and a base 33 is connected between the two support blocks 32. A fixing block 34 is connected to the upper left end of the auxiliary cylinder 1, and a rotating column 35 is rotatably connected to the top of the fixing block 34. A blade 36 is connected to the right end of the rotating column 35. The freezing frame 31 contains an aqueous solution of ethylene glycol. The support blocks 32 are connected to the left and right ends of the auxiliary cylinder 1, and a base 33 is connected between the two support blocks 32. A fixing block 34 is connected to the upper left end of the auxiliary cylinder 1. Block 34 is fixed and rotatably connected to the top of a rotating column 35. The right end of the rotating column 35 is connected to a blade 36. The high-concentration ethylene glycol aqueous solution in the freezing frame 31 has a low freezing point and can remain liquid at a low temperature and continuously absorb heat, which can effectively accelerate the freezing speed of the sample and facilitate rapid freezing and shaping of the sample. The support block 32 cooperates with the base 33 to stably support the auxiliary cylinder 1 and enhance the overall stability of the device. The fixed block 34 supports the rotation of the rotating column 35, and the rotating column 35 drives the blade 36 to rotate, which can cut the sample and improve the convenience of sampling.
[0025] Please see Figures 2-5 In this embodiment, a handle 37 is connected to the left end of the rotating column 35, and an anti-slip ring 38 is connected to the bottom of the circular frame 21. Pushing the handle 37 causes the rotating column 35 to rotate within the fixed block 34. The rotation of the rotating column 35 causes the blade 36 to rotate. The anti-slip ring 38 is used to control the glue tension. The support block 32 and the base 33 are used to support the auxiliary cylinder 1. The handle 37 makes the rotation of the rotating column 35 easier and more convenient, and facilitates flexible control of the blade 36 to cut the sample. The anti-slip ring 38 can reduce glue leakage by controlling the glue tension. To prevent overflow or excessive shrinkage and ensure the embedding effect, the support block 32 and the base 33 further enhance the support stability of the auxiliary cylinder 1, ensuring that the device is not easily shaken during the sampling process. The handle 37 makes the rotation operation of the rotating column 35 easier and more convenient, and facilitates flexible control of the blade 36 to cut the sample. The anti-slip ring 38 can reduce glue overflow or excessive shrinkage by controlling the glue tension, ensuring the embedding effect. The support block 32 and the base 33 further enhance the support stability of the auxiliary cylinder 1, ensuring that the device is not easily shaken during the sampling process.
[0026] During operation, embedding adhesive and sample are first poured into the circular frame 21. The anti-slip ring 38 at the bottom of the circular frame 21 controls the adhesive tension, reducing adhesive overflow or excessive shrinkage and ensuring the embedding effect. Then, the device is placed in the freezer. The high-concentration ethylene glycol aqueous solution in the freezing frame 31, due to its low freezing point, remains liquid at a lower temperature and continuously absorbs heat, accelerating the freezing speed of the sample and facilitating rapid freezing and shaping. When sample processing is required, the rotating ring 210 at the outer end of the auxiliary cylinder 1 is rotated. The rotating ring 210 drives the connecting column 211 and the pawl 212 at the bottom to rotate synchronously. The pawl 212, with the help of the return shaft, makes close contact with the ratchet 29 and pushes it to rotate. When the ratchet 29 rotates, it drives the threaded post 26 at the bottom to rotate. Since the cylinder 25 is threadedly connected to the threaded post 26, and the sliding rods 28 at both ends of the cylinder 25 slide in the grooves 27 inside the auxiliary cylinder 1 to act as guides, the rotation of the threaded post 26 will drive the cylinder 25 to move upward steadily, thereby pushing the rubber stopper 24 and the push plate 23 to move upward. The push plate 23 pushes out the embedding adhesive, pushes the handle 37 at the left end of the rotating post 35, and drives the rotating post 35 to rotate in the fixed block 34. The blade 36 at the right end of the rotating post 35 rotates accordingly, which can cut the sample. The operation is labor-saving, flexible and convenient. This design greatly reduces the operation time and avoids sample damage caused by forced removal.
[0027] Through the above steps, by setting the rotating ring 210, connecting post 211, pawl 212, ratchet 29 and threaded post 26, cylinder 25 and push plate 23 to cooperate with each other, the sample and embedding adhesive can be successfully removed. Rotating the rotating ring 210 causes the connecting column 211 and the pawl 212 to rotate synchronously. The pawl 212, with the help of the return shaft, engages and pushes the ratchet 29 to rotate, which in turn drives the threaded column 26 to rotate. Since the cylinder 25 and the threaded column 26 are threadedly connected, and the slide bar 28 acts as a guide in the slide groove 27, the cylinder 25 will move smoothly upward, pushing the rubber stopper 24 and the push plate 23 to eject the embedding adhesive. This process can shorten the operation time, improve efficiency, prevent sample damage to ensure its integrity, provide reliable evidence for subsequent testing, and enhance the practicality of the device. This solves the problem of existing animal liver tissue rapid cryosamplers, where the embedding adhesive adheres tightly to the inner wall after freezing and there is no convenient ejection structure, making it difficult to remove the sample and embedding adhesive, which is both time-consuming and may damage the sample if forcibly removed.
Claims
1. A rapid cryogenic sampler for animal liver tissue, comprising an auxiliary tube (1); characterized in that: It also includes a circular frame (21) installed on the top of the auxiliary cylinder (1), with a push hole (22) at the bottom of the circular frame (21), a push plate (23) inside the push hole (22), a rubber plug (24) connected to the bottom of the push plate (23), a cylinder (25) connected to the bottom of the rubber plug (24), a threaded column (26) connected to the inside of the cylinder (25), a sliding groove (27) on the left and right sides inside the auxiliary cylinder (1), a sliding rod (28) connected to the left and right ends of the cylinder (25), the sliding rod (28) and the sliding groove (27) being slidably connected, and a ratchet (29) extending from the bottom of the threaded column (26) to the bottom of the auxiliary cylinder (1) being rotatably connected to the auxiliary cylinder (1). A rotating ring (210) is rotatably connected to the outer end of the cylinder (1). A connecting post (211) is connected to the bottom of the rotating ring (210). A pawl (212) is rotatably connected to the bottom of the connecting post (211) via a return shaft. The pawl (212) is movably connected to the ratchet (29). The rotating ring (210) rotates, causing the connecting post (211) and the pawl (212) to rotate. The pawl (212) pushes the ratchet (29) to rotate at the bottom of the auxiliary cylinder (1). The ratchet (29) rotates, driving the slide bar (28). The slide bar (28) rotates, pushing the cylinder (25) to move upward. The cylinder (25) pushes the slide bar (28) to slide in the groove (27). The cylinder (25) moves upward, pushing the rubber plug (24) to push the push plate (23) out.
2. The animal liver tissue rapid cryopreservation sampler according to claim 1, characterized in that: A freezing frame (31) is connected to the upper side of the outer end of the auxiliary cylinder (1), and support blocks (32) are connected to the left and right ends of the auxiliary cylinder (1).
3. The animal liver tissue rapid cryopreservation sampler according to claim 2, characterized in that: A chassis (33) is connected between the two support blocks (32), and a fixing block (34) is connected to the upper left side of the auxiliary cylinder (1).
4. The animal liver tissue rapid cryopreservation sampler according to claim 3, characterized in that: The top of the fixed block (34) is rotatably connected to a rotating column (35), and the right end of the rotating column (35) is connected to a blade plate (36).
5. The animal liver tissue rapid cryopreservation sampler according to claim 4, characterized in that: A handle (37) is connected to the left end of the rotating column (35), and an anti-slip ring (38) is connected to the bottom of the round frame (21).
6. The animal liver tissue rapid cryopreservation sampler according to claim 5, characterized in that: Pushing the handle (37) causes the rotating column (35) to rotate within the fixed block (34), and the rotation of the rotating column (35) causes the blade (36) to rotate.
7. The animal liver tissue rapid cryopreservation sampler according to claim 6, characterized in that: The anti-slip ring (38) is used to control the glue tension, and the support block (32) and the chassis (33) are used to support the auxiliary cylinder (1).