A biological cell separation device

Abstract

The utility model provides a kind of biological cell separation device, it is related to biological cell separation technical field, including separation seat, the separation seat side top edge is fixedly installed with several connection rings of the annular array distribution around separation seat center, any the coaxial distribution and fixed mounting in the base of the connection ring below separation seat side middle position is provided with, and any the circular ring and rotating seat are arranged in the position above and below of the connection ring, by being provided with several roller frame in circular ring and rotating seat, the clamping force even distribution is realized in the tube orifice and tube bottom of test tube using several roller frame resistance test tube surface, avoid test tube swing in the process of rotation, and using rotating seat rotation drives the revolution of several roller frame inside to further realize test tube rotation, the tire of roller frame in circular ring can rotate independently, when test tube rotation, the tire of roller frame in circular ring rotates under the action of friction force, further stabilize test tube tube orifice.

Description

Technical Field

[0001] This utility model relates to the field of biological cell separation technology, and in particular to a biological cell separation device. Background Technology

[0002] Biological cell isolation is an important step in cell biology research. Its purpose is to obtain specific cell types from complex biological tissues or cell populations for subsequent experimental analysis.

[0003] Therefore, a prior art biological cell separation device, disclosed in CN220564572U, involves placing a test tube through a hole on a support plate. A second drilling gear drives a first gear to rotate, which in turn drives a nut to rotate. The nut's rotation moves a screw threaded to it, thereby moving a clamping plate and an anti-slip pad to hold the test tube in place. The test tube is then secured. The cap is then placed on top, and a lever is pulled away from the locking block. The lever is then released, and a spring causes the lever to engage with the locking block, securing the cap. Finally, the motor is turned on, causing the support plate to rotate, which in turn rotates the positioning plate, generating centrifugal force.

[0004] However, as shown in the attached diagram of the existing technology, the holes on the positioning plate are linearly distributed along its diameter, while the test tubes inside the holes are fixedly installed. Therefore, when the positioning plate rotates, only a few holes are involved in the revolution, thus causing the fixed test tubes inside the holes to revolve. During this revolution, the liquid and cells inside the test tubes are subjected to centrifugal force, but this centrifugal force is directed along the radial direction of the test tube. If the test tubes do not rotate, the distribution of liquid and cells within the test tubes may be uneven, leading to unsatisfactory separation results. Utility Model Content

[0005] The purpose of this invention is to solve the problems existing in the prior art by proposing a biological cell separation device.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a biological cell separation device, comprising a separation seat, wherein a plurality of connecting rings are fixedly installed on the top edge of the side of the separation seat and arranged in a ring array around the center of the separation seat; a base is coaxially distributed and fixedly installed at the middle position of the side of the separation seat below any of the connecting rings; and a circular ring and a rotating seat are respectively arranged above and below any of the connecting rings; the center of the outer bottom surface of the rotating seat extends downward through the base at the lower position and a spur gear is fixedly installed at the extension; a spur gear ring is rotatably connected to the outer side of the separation seat and meshes with the spur gear; and a plurality of roller frames for rolling connection with the outer wall of a test tube are engaged in both the rotating seat and the circular ring; the central axis of the roller frame is arranged parallel to the central axis of the circular ring.

[0007] Preferably, a screw is provided through the side of the ring and the rotating seat at any position of the roller frame, and a matching conical tooth ring that is threadedly connected to the outer side of the ring and the rotating seat is rotatably connected to the screw. The screw is arranged perpendicular to the central axis of the ring, and one end of the screw is fixedly connected to the surface of the roller frame body at the adjacent position.

[0008] Preferably, both the outer edges of the circular ring and the rotating seat are rotatably connected to a driving bevel gear ring, and each of the driving bevel gear rings is engaged with a mating bevel gear ring located at the same outer edge position.

[0009] Preferably, a longitudinally telescopic electric telescopic rod is fixedly installed on one side of the inner wall of both the ring and the rotating seat, and a damping plate for surface damping cooperation with the adjacent drive bevel ring is fixedly installed at the telescopic end of the electric telescopic rod.

[0010] Preferably, the telescopic end of the electric telescopic rod located within the ring is also rotatably connected to a bearing for pressing the test tube opening.

[0011] Preferably, a plurality of hollow inserts are fixedly installed on the bottom edge of the circular ring, penetrating the top of the lower connecting ring, and an electric push rod is fixedly installed on the top of the connecting ring, which is horizontally set and used to be inserted into the hollow part of the surface of one of the hollow inserts.

[0012] Preferably, a motor is fixedly mounted on the top surface of one of the bases, and the motor spindle is fixedly connected to the center of the end face of a spur gear on the top surface of the same base.

[0013] Compared with the prior art, the advantages and positive effects of this utility model are as follows:

[0014] 1. In this utility model, by setting several roller frames inside the ring and the rotating seat, the clamping force is evenly distributed between the mouth and bottom of the test tube by the contact of the roller frames with the test tube surface, which avoids the test tube from swaying and shaking during the rotation. Furthermore, the rotation of the rotating seat drives the internal roller frames to revolve, thereby realizing the rotation of the test tube. The roller frame tires inside the ring can rotate independently. When the test tube rotates, the roller frame tires inside the ring rotate under the action of friction, further stabilizing the mouth of the test tube.

[0015] 2. In this invention, the distance between the ring and the rotating seat can be flexibly adjusted through the cooperation of the electric push rod and the hollow insert, thus accommodating test tubes of different heights. This design allows the device to be compatible with test tubes of various sizes without the need to replace or adjust other components. Attached Figure Description

[0016] Figure 1 This invention provides a three-dimensional structural schematic diagram of a biological cell separation device;

[0017] Figure 2This invention provides a schematic diagram of the structure of a connecting ring for a biological cell separation device;

[0018] Figure 3 This invention proposes a biological cell separation device. Figure 2 A schematic diagram of the structure viewed from below;

[0019] Figure 4 This invention proposes a biological cell separation device. Figure 2 A cross-sectional structural diagram.

[0020] Legend: 1. Separator; 2. Ring; 3. Bearing; 4. Hollowed-out insert; 5. Spur gear ring; 6. Roller frame; 7. Matching bevel gear ring; 8. Drive bevel gear ring; 9. Base; 10. Screw; 11. Spur gear; 12. Motor; 13. Rotating seat; 14. Electric telescopic rod; 15. Damping plate; 16. Electric push rod; 17. Connecting ring. Detailed Implementation

[0021] To better understand the above-mentioned objectives, features, and advantages of this utility model, the present utility model will be further described below with reference to the accompanying drawings and embodiments. It should be noted that, unless otherwise specified, the embodiments and features described in these embodiments can be combined with each other.

[0022] Many specific details are set forth in the following description in order to provide a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed in the following specification.

[0023] like Figures 1-4As shown, a biological cell separation device includes a separation seat 1. Several connecting rings 17 are fixedly installed on the top edge of the side of the separation seat 1, arranged in a circular array around the center of the separation seat 1. A base 9 is coaxially distributed and fixedly installed at the center of the side of the separation seat 1 below any connecting ring 17. A circular ring 2 and a rotating seat 13 are respectively located above and below any connecting ring 17. The center of the outer bottom surface of the rotating seat 13 extends downward through the base 9 below, and a spur gear 11 is fixedly installed at the extension. When placing a test tube, the bottom of the test tube passes through the circular ring 2 and the connecting ring 17 from top to bottom and is finally placed in the rotating seat 13 below. A spur gear ring 5 is rotatably connected to the outer side of the separation seat 1, meshing with the spur gear 11. Several roller frames 6 are engaged in both the rotating seat 13 and the circular ring 2 for rolling contact with the outer wall of the test tube, ensuring that the roller frames 6 cannot rotate but can only move horizontally. The central axis of the roller frames 6 is parallel to the central axis of the circular ring 2. After the test tube is placed, the roller frames 6 in the circular ring 2 and the rotating seat 13 move. The tire contacts the surface of the test tube, and the tire portion of the roller frame 6 inside the rotating seat 13 is fixedly installed and cannot rotate. The tire portion of the roller frame 6 inside the ring 2 can rotate independently. A motor 12 is fixedly installed on the top surface of one of the bases 9. The main shaft of the motor 12 is fixedly connected to the center of the end face of the spur gear 11 on the top surface of the same base 9. Therefore, after the test tube is clamped by the ring 2 and the roller frame 6 inside the rotating seat 13, the motor 12 drives the corresponding spur gear 11 to rotate, while the spur gears 11 in other positions rotate. Synchronous rotation under the meshing transmission of the spur ring 5 enables all rotating seats 13 around the separating seat 1 to rotate simultaneously. Since the tires of the roller frame 6 inside the rotating seat 13 cannot rotate, when the rotating seat 13 rotates, the several roller frames 6 inside will revolve synchronously, thereby achieving the rotation of the test tube under the clamping action. When the test tube rotates, the area near the mouth of the test tube will not sway or shake under the support of the tires of the several roller frames 6 inside the ring 2, but will be driven to rotate by the frictional force.

[0024] A screw 10 is installed through the sides of both the ring 2 and the rotating seat 13 at any position of the roller frame 6. A mating beveled ring 7, threadedly connected to the screw 10, is rotatably connected to the outer sides of both the ring 2 and the rotating seat 13. The screw 10 is perpendicular to the central axis of the ring 2, and one end of the screw 10 is fixedly connected to the surface of the adjacent roller frame 6. A driving beveled ring 8 is rotatably connected to the outer sides of both the ring 2 and the rotating seat 13. Each driving beveled ring 8 meshes with a mating beveled ring 7 located on the same outer side. In actual operation, when the driving beveled ring 8 on the outer side of the ring 2 is rotated, all the mating beveled rings 7 on the outer side of the ring 2 will rotate synchronously. Utilizing the threaded connection between the mating beveled ring 7 and the screw 10, the screw 10 can drive the roller frame 6 to move horizontally as the mating beveled ring 7 rotates. A longitudinally telescopic electric telescopic rod 14 is fixedly installed on one side of the inner wall of both the ring 2 and the rotating seat 13. The telescopic end of the electric telescopic rod 14 is fixedly installed with a device for connecting with adjacent… The damping plate 15, which is in contact with the surface of the drive bevel ring 8, disengages under the pushing action of the extension of the electric telescopic rod 14 when the drive bevel ring 8 needs to rotate. After the position of the roller frame 6 is adjusted, the damping plate 15 descends under the traction of the contraction of the electric telescopic rod 14 to re-closely contact the surface of the drive bevel ring 8. Under the action of friction, the drive bevel ring 8 is prevented from rotating, thus fixing the position of the roller frame 6. The telescopic end of the electric telescopic rod 14 located in the ring 2 is also rotatably connected to the bearing 3 for pressing the mouth of the test tube. When the damping plate 15 in the ring 2 descends, the bearing 3 descends synchronously to close contact with the mouth of the test tube, preventing the test tube from jumping up and down. In addition, the outer ring of the bearing 3 in this scheme is rotatably connected to the telescopic end of the electric telescopic rod 14, and the inner ring is used to press the mouth of the test tube. When the test tube 3 rotates, it drives the inner ring of the bearing 3 to rotate. When picking up and putting down the test tube, the bearing 3 is horizontally moved to disengage the bearing 3 from the top of the ring 2.

[0025] A number of hollowed-out inserts 4 are fixedly installed on the bottom edge of the ring 2, passing through the top of the connecting ring 17 below. A horizontally set electric push rod 16 is fixedly installed on the top of the connecting ring 17 for insertion into the hollowed-out part of one of the hollowed-out inserts 4. Depending on the height of the test tube, when the telescopic end of the electric push rod 16 is retracted to separate from the hollowed-out insert 4, the ring 2 is pulled up and down to realize the synchronous lifting and lowering of the hollowed-out insert 4. The distance between the ring 2 and the rotating seat 13 is adjusted according to the height of the test tube. After the height adjustment is completed, the telescopic end of the electric push rod 16 is extended again to insert into the hollowed-out part on the hollowed-out insert 4 to fix the relative position of the hollowed-out insert 4 and the connecting ring 17, that is, to fix the relative position of the ring 2 and the rotating seat 13 in terms of the vertical position of the connecting ring 17.

[0026] The working principle is as follows: The distance between the upper and lower positions of the connecting ring 17 and the rotating seat 13 is adjusted according to the height of the test tube. The bottom of the test tube 3 is passed through the ring 2 and connecting ring 17 from top to bottom and finally placed in the rotating seat 13 below. Then, with the damping plate 15 separated from the driving bevel ring 8, the roller frame 6 inside the rotating ring 2 is pressed against the periphery of the test tube opening by the driving bevel ring 8 on the rotating ring 2. The roller frame 6 inside the rotating seat 13 is pressed against the periphery of the bottom of the test tube by the driving bevel ring 8 on the rotating seat 13. The motor 12 drives the corresponding spur gear 11 to rotate. The spur gears 11 in other positions rotate synchronously under the meshing transmission of the spur ring 5. This allows all the rotating seats 13 around the separation seat 1 to rotate simultaneously. Since the tires of the roller frame 6 inside the rotating seat 13 cannot rotate, when the rotating seat 13 rotates, several roller frames 6 inside will revolve synchronously, thus achieving the rotation of the test tube under the clamping action, achieving the separation of the physical cells inside the test tube.

[0027] The wiring diagrams of the motor 12, electric telescopic rod 14, and electric push rod 16 in this utility model are common knowledge in the field, and their working principles are known technologies. The appropriate model is selected according to actual use. Therefore, the control method and wiring layout of the motor 12, electric telescopic rod 14, and electric push rod 16 will not be explained in detail.

[0028] The above description is merely a preferred embodiment of the present utility model and is not intended to limit the present utility model in any other way. Any person skilled in the art may make changes or modifications to the above-disclosed technical content to create equivalent embodiments for application in other fields. However, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present utility model without departing from the technical solution of the present utility model shall still fall within the protection scope of the technical solution of the present utility model.

Claims

1. A biological cell separation device, characterized in that: The device includes a separation seat (1), on which several connecting rings (17) are fixedly installed in a ring array around the center of the separation seat (1) at the top edge of the side. A base (9) is coaxially distributed and fixedly installed in the middle of the side of the separation seat (1) below any of the connecting rings (17). A circular ring (2) and a rotating seat (13) are respectively provided above and below any of the connecting rings (17). The center of the outer bottom surface of the rotating seat (13) extends downward through the base (9) below and a spur gear (11) is fixedly installed at the extension. A spur gear ring (5) is rotatably connected to the outer side of the separation seat (1) and meshes with the spur gear (11). Several roller frames (6) for rolling connection with the outer wall of the test tube are snapped in the rotating seat (13) and the circular ring (2). The central axis of the roller frame (6) is parallel to the central axis of the circular ring (2).

2. The biological cell separation device according to claim 1, characterized in that: The ring (2) and the rotating seat (13) are both provided with screws (10) at the positions of any roller frame (6), and the outer sides of the ring (2) and the rotating seat (13) are rotatably connected with matching bevel rings (7) that are threadedly connected to the screws (10). The screws (10) are perpendicular to the central axis of the ring (2), and one end of the screws (10) is fixedly connected to the surface of the roller frame (6) at the adjacent position.

3. The biological cell separation device according to claim 2, characterized in that: Both the outer sides of the ring (2) and the rotating seat (13) are rotatably connected to a driving bevel ring (8), and each of the driving bevel rings (8) is engaged with a mating bevel ring (7) located on the same outer side.

4. The biological cell separation device according to claim 3, characterized in that: Both the inner wall of the ring (2) and the rotating seat (13) are fixedly installed with a longitudinally telescopic electric telescopic rod (14), and the telescopic end of the electric telescopic rod (14) is fixedly installed with a damping plate (15) for surface damping cooperation with the adjacent drive bevel ring (8).

5. The biological cell separation device according to claim 4, characterized in that: The telescopic end of the electric telescopic rod (14) located inside the ring (2) is also rotatably connected to a bearing (3) for pressing the test tube opening.

6. The biological cell separation device according to claim 1, characterized in that: The bottom edge of the ring (2) is fixedly installed with several hollow inserts (4) that pass through the top of the lower connecting ring (17). The top of the connecting ring (17) is fixedly installed with a horizontally set electric push rod (16) for insertion into the hollow part of the surface of one of the hollow inserts (4).

7. The biological cell separation device according to claim 1, characterized in that: One of the bases (9) has a motor (12) fixedly mounted on its top surface, and the main shaft of the motor (12) is fixedly connected to the center of the end face of the spur gear (11) on the top surface of the same base (9).

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