Embryo sample collection and lysis integrated box
By designing an integrated embryo sample collection and lysis kit, the problem of cumbersome low-temperature operation in the embryo sample collection process in the existing technology is solved, realizing the continuity of the low-temperature environment and the flexibility of multiple sets of experiments, and simplifying the operation process.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- XIAMEN MATERNAL & CHILD HEALTH HOSPITAL (XIAMEN EUGENICS & POSTNATAL CARE SERVICE CENT XIAMEN UNIV AFFILIATED WOMEN & CHILDRENS HOSPITAL XIAMEN LIN QIAOZHI WOMEN & CHILDRENS HOSPITAL)
- Filing Date
- 2025-07-08
- Publication Date
- 2026-06-09
AI Technical Summary
In existing technologies, the embryo sample collection process requires operation one by one in a low-temperature environment, and independent ice buckets cannot meet the needs of long-term and multiple experiments, resulting in cumbersome operation.
Design an integrated embryo sample collection and lysis box, comprising an outer shell, an ice box, and a PCR plate. The ice box is connected to the slot via a locking block, and has multiple tube slots on the top. The PCR plate is embedded in the accommodating space, and a baffle controls the movement of the ice box, achieving continuous low-temperature environment and meeting the needs of multiple sets of experiments.
It enables continuity and flexibility in multiple experiments under low-temperature conditions, meets the requirements for long-term low-temperature use, and simplifies the operation process.
Smart Images
Figure CN224337550U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of laboratory equipment technology, and in particular to an integrated box for collecting and splitting embryo samples. Background Technology
[0002] After embryo biopsy, the collected samples need to be placed into PCR tubes. This process must be performed on an ice pack, while cell lysis reagent preparation is done in the reagent preparation area of the PGT laboratory. The lysis buffer is removed from the -20°C freezer and thawed at room temperature. A new 0.2 mL centrifuge tube is taken, and the thawed lysis buffer is vortexed and briefly centrifuged for 15 seconds using a handheld centrifuge. 5 μL of lysis buffer is added to the bottom of the prepared tube. Cell lysis and sample loading are performed in the biosafety cabinet in the WGA area of the PGT laboratory. The operator removes the sample to be amplified and the positive control from the 4°C freezer, briefly centrifuges the sample and positive control sample for 15 seconds on the lab bench, and then removes them from the centrifuge and places them on a PCR plate. Current methods require individual PCR tubes to be processed one by one in a low-temperature environment, which is cumbersome. Furthermore, commonly used low-temperature methods often involve separate ice packs, which cannot meet the needs of long-term use and repeated experiments. Therefore, a replaceable ice pack is needed to design an integrated embryo sample collection and lysis box that can increase experimental time and meet low-temperature conditions. Utility Model Content
[0003] The purpose of this invention is to provide an integrated kit for embryo sample collection and lysis.
[0004] To achieve the above objectives, the present invention adopts the following technical solution:
[0005] An integrated embryo sample collection and lysis box includes an outer shell, an ice box, and a PCR plate. The outer shell has two side plates and a rear plate. The two side plates are symmetrically arranged at both ends of the rear plate to form a C-shaped structure. Each of the two side plates has a slot on its inner side. The ice box is a square box with locking blocks at both ends that engage with the slots, and multiple tube slots arrayed on the top. The ice box engages with the slots through the locking blocks and enters the outer shell along them. The top of the ice box and the outer shell have a accommodating space. The PCR plate is placed in the accommodating space and has tubes that can be inserted into the tube slots. Each tube is filled with embryo collection fluid. The side plates have rotating holes, and baffles are rotatably installed in the rotating holes to restrict the movement of the ice box by rotating the baffles.
[0006] Furthermore, the top of the two side plates of the outer shell are provided with recesses facing inward, and the two sides of the PCR plate are provided with protrusions that cooperate with the recesses. The PCR activity is restricted by the interlocking of the protrusions and recesses.
[0007] Furthermore, the outer casing also includes two anti-slip strips, which are arranged in parallel on the bottom of the outer casing, and the two ends of the anti-slip strips are respectively glued to the bottom of the two side panels.
[0008] Furthermore, the anti-slip strip is made of rubber or plastic, and its bottom surface is frosted.
[0009] Furthermore, the bottom of the ice box is provided with an opening for water to enter and exit, and a plug for switching on / off is provided in the opening.
[0010] By adopting the above technical solution, this utility model has the following advantages compared with the prior art:
[0011] This invention achieves a low-temperature environment for PCR plates using an ice box. The ice box can be replaced at any time, and a new ice box can be replaced when it can no longer meet the low-temperature requirements, thus meeting the needs of long-term low-temperature use. At the same time, the PCR plate is equipped with multiple tubes that can be inserted into the ice box to meet the needs of multiple sets of experiments. Attached Figure Description
[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model;
[0013] Figure 2 This is a bottom view of the present invention;
[0014] Figure 3 This is an exploded structural diagram of the present invention;
[0015] Figure 4 This is an exploded view of the present invention from another angle.
[0016] Explanation of reference numerals in the attached figures:
[0017] Outer shell 1; Ice box 2; PCR plate 3; Side plate 11; Back plate 12; Slot 13; Baffle 14; Recess 15; Anti-slip strip 16; Locking block 21; Tube groove 22; Plug 23; Tube body 31; Protrusion 32. Detailed Implementation
[0018] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.
[0019] It should be noted that in this utility model, the terms "upper", "lower", "left", "right", "vertical", "horizontal", "inner", and "outer" are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or element of this utility model must have a specific orientation, and therefore should not be construed as a limitation of this utility model.
[0020] Example
[0021] Cooperate Figures 1 to 4 As shown, this utility model discloses an integrated embryo sample collection and lysis box, including an outer shell 1, an ice box 2, and a PCR plate 3. The outer shell 1 has two side plates 11 and a rear plate 12. The two side plates 11 are symmetrically arranged at both ends of the rear plate 12 to form a C-shaped structure. The inner side of each side plate 11 is provided with a slot 13. The ice box 2 is a square box body with a locking block 21 at both ends that engages with the slot 13, and multiple tube slots 22 are arrayed on the top. The ice box 2 engages with the slot 13 through the locking block 21 and enters the outer shell 1 along it. The top of the ice box 2 and the outer shell 1 have a accommodating space. The PCR plate 3 is placed in the accommodating space and has a tube body 31 that is inserted into the tube slot 22. Each tube body is filled with embryo collection fluid. The type of embryo collection fluid is not limited in this embodiment. The side plate 11 is provided with a rotating hole, and a baffle 14 is rotatably installed in the rotating hole to restrict the movement of the ice box 2 by rotating the baffle 14.
[0022] The baffle 14 is provided with a rotating shaft that is rotatably installed in the rotating hole. The rotating shaft can be a screw, which is tightened when the baffle is set horizontally and loosened when it is set vertically.
[0023] The side panels 11 and rear panels 12 of the outer shell 1 are preferably acrylic sheets, which are glued together or integrally formed. An insulation layer can also be provided on their outer surface. The material of the insulation layer is not limited in this embodiment and can be foam.
[0024] In this embodiment, the tube slot 22 is an ice box 2 with a tube opening, and a conical or cylindrical tube adapted to the tube body specifications is provided inside the tube opening. The number of tube slots 22 and tube bodies 31 is the same for the same specifications, but the number for different specifications is not limited in this embodiment. Different experimental needs can be met by selecting the corresponding number of ice boxes 2 and PCR plates 3.
[0025] The top of the two side plates 11 of the outer shell 1 is provided with recesses 15 facing inward, and the two sides of the PCR plate 3 are provided with protrusions 32 that cooperate with the recesses 15. The PCR activity is restricted by the interlocking of the protrusions 32 with the recesses 15.
[0026] The outer casing 1 also includes two anti-slip strips 16, which are arranged in parallel at the bottom of the outer casing 1. The two ends of the anti-slip strips 16 are respectively glued to the bottom of the two side panels 11.
[0027] The anti-slip strip 16 is made of rubber or plastic, and its bottom surface is frosted.
[0028] The bottom of the ice box 2 has an opening for water to enter and exit, and a plug 23 for switching on / off is provided in the opening.
[0029] In this embodiment, the PCR plate 3 is kept in a low-temperature environment by using an ice box 2. The ice box 2 can be replaced at any time, and a new ice box 2 can be replaced when it can no longer meet the low-temperature requirements, thus meeting the needs of long-term low-temperature use. At the same time, the PCR plate 3 is equipped with multiple tubes that can be inserted into the ice box, which can meet the needs of multiple sets of experiments.
[0030] The above description is merely a preferred embodiment of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.
Claims
1. An integrated kit for embryo sample collection and lysis, characterized in that: The device includes an outer shell, an ice box, and a PCR plate. The outer shell has two side panels and a rear panel. The two side panels are symmetrically arranged at both ends of the rear panel to form a C-shaped structure. Each side panel has a slot on its inner side. The ice box is a square box with locking blocks at both ends that engage with the slots, and multiple tube slots arranged on the top. The ice box engages with the slots via the locking blocks and enters the outer shell. There is a space between the top of the ice box and the outer shell. The PCR plate is placed in the space and has tubes that can be inserted into the tube slots. Each tube is filled with embryo collection fluid. The side panels have rotating holes, and baffles are rotatably installed in the rotating holes to restrict the movement of the ice box by rotating the baffles.
2. The embryo sample collection and lysis integrated box as described in claim 1, characterized in that: The top of the two side plates of the outer shell are provided with recesses facing inward, and the two sides of the PCR plate are provided with protrusions that cooperate with the recesses. The PCR activity is restricted by the interlocking of the protrusions and recesses.
3. The embryo sample collection and lysis integrated box as described in claim 1, characterized in that: The outer casing also includes two anti-slip strips, which are arranged in parallel on the bottom of the outer casing. The two ends of the anti-slip strips are respectively glued to the bottom of the two side panels.
4. The embryo sample collection and lysis integrated box as described in claim 3, characterized in that: The anti-slip strip is made of rubber or plastic, and its bottom surface is frosted.
5. The embryo sample collection and lysis integrated box as described in claim 1, characterized in that: The bottom of the ice box has an opening for water to enter and exit, and a plug for switching on / off is provided in the opening.