Real-time fluorescent ultrafast photothermal PCR amplification detection device

Abstract

This utility model discloses a real-time fluorescence ultrafast photothermal PCR amplification and detection device, belonging to the field of intelligent medical testing instrument technology. It includes a laser probe and an adjustable laser power supply. An infrared temperature sensor is coaxially fixed to one side of the laser probe. A chip chamber is located below the laser probe, and a PCR chip is installed within the chip chamber. The PCR chip contains photothermal nanomaterials. A fluorescence feedback component is located below the chip chamber, and an imaging component is connected to the end of the fluorescence feedback component furthest from the chip chamber. This invention solves the technical problems of existing PCR amplification and detection devices, such as long amplification times, inability to observe temperature in real time, and inability to ensure the temperature conditions for thermal cycling amplification of nucleic acid molecules.

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