An analytical system for automated detection of cryoglobulinemia

Abstract

The utility model discloses an analysis system for the automatic detection cryoglobulinemia, wherein the key component is a scanning detection device. The scanning detection device mainly comprises a first base, a first transmission mechanism is arranged on the first base; a bearing seat is also installed on the first base, and is used for loading a reaction cup. It is worth mentioning that the scanning detector is connected with the first transmission mechanism in transmission, and under the driving of the first transmission mechanism, the scanning detector can perform accurate reciprocating motion, so that comprehensive scanning detection of the reaction cup on the bearing seat is carried out. During work, the scanning detector moves up and down along a specific path, and in the moving process, the serum state in the reaction cup is scanned and detected in detail to determine whether the sample in the cup appears cloud-like turbidity or precipitation phenomenon. Compared with the traditional manual interpretation, this scanning detection method has obvious advantages.

Description

Technical Field

[0001] This utility model belongs to the field of detection equipment technology, specifically relating to an automated analysis system for detecting cryoglobulinemia. Background Technology

[0002] Cryoglobulin was first reported in 1933, and in 1947 Lerner confirmed that it was essentially a gamma globulin.

[0003] This gamma globulin is also an immunoglobulin, present in many clinical diseases, and can fix complement to produce an inflammatory response, similar to diseases caused by immune complexes.

[0004] Clinical diagnosis: It is mainly associated with hepatitis C, cirrhosis, primary macroglobulinemia, rheumatoid arthritis, Sjögren's syndrome, systemic lupus erythematosus, vasculitis, cytomegalovirus infection, infectious mononucleosis, etc.

[0005] Cryoglobulinemia is more widely used in screening for suspected diseases. It can cause a variety of symptoms, including characteristic purpura, limb ischemia, renal failure, peripheral neuropathy secondary to intestinal ischemia, abdominal pain, and arthralgia. It is also used in physical examinations of middle-aged and elderly individuals. However, cryoglobulin testing is not fully utilized in clinical practice. This test has been neglected by clinical laboratories and clinicians for several reasons, such as the long time required for serum cryoglobulin analysis in the laboratory, difficulties in obtaining optimal sampling conditions, and a failure to recognize that even significantly low levels of cryoglobulin can be associated with severe symptoms in some patients. The most important variable affecting the standardization of cryoglobulin testing is inappropriate sample handling. Relying on manual testing methods, the interpretation of results still depends on human intervention, is susceptible to endogenous and exogenous interference factors, and manual interpretation is characterized by subjectivity, inefficiency, reliance on specialized knowledge, and sensitivity to specific conditions.

[0006] Currently, cryoglobulin detection relies primarily on manual operation, requiring subjective judgment of results through visual inspection. The quantification and typing of cryoglobulins remain significant challenges for clinical laboratories. Because the interpretation and calculation process is highly dependent on manual intervention, the detection is susceptible to endogenous and exogenous interference factors, exhibiting problems such as poor objectivity, low efficiency, stringent requirements for specialized knowledge, and extreme sensitivity to specific conditions. Furthermore, the result output process suffers from incomplete interpretation and a lack of standardization.

[0007] Therefore, developing an automated analytical system and operating method for detecting cryoglobulinemia has become an urgent need for clinical laboratories. This will not only help improve the accuracy and objectivity of the test, but also significantly increase testing efficiency, reduce reliance on specialized knowledge, and promote the standardization and normalization of cryoglobulin testing, thereby better serving clinical diagnosis and treatment. Utility Model Content

[0008] The purpose of this invention is to provide an automated analytical system for detecting cryoglobulinemia, which solves the above-mentioned technical problems. By moving the detector up and down, the system scans and detects the serum in the reaction cup during the movement to determine whether there is cloudiness or precipitation in the sample. This scanning detection method is easy to implement, has high stability, avoids the subjectivity and low efficiency caused by manual interpretation, and its movement path is controllable, which is conducive to the standardized output of results.

[0009] To achieve the above-mentioned objectives, the technical solution adopted by this utility model is as follows:

[0010] An automated analytical system for detecting cryoglobulinemia includes a scanning detection device. The scanning detection device comprises a first base, a first transmission mechanism mounted on the first base, a support seat mounted on the first base for loading a reaction cup, and a scanning detector connected to the first transmission mechanism to perform reciprocating motion to scan the reaction cup. Specifically, the scanning detector operates by moving up and down to scan. By moving the scanning detector up and down and scanning the serum in the reaction cup during this process, it determines whether the sample in the reaction cup exhibits cloudy turbidity or precipitation. This scanning detection method is easy to implement, highly stable, and avoids the subjectivity and low efficiency problems associated with manual interpretation. Furthermore, its controllable movement path facilitates standardized result output.

[0011] Preferably, the reaction cup is arranged vertically, and the scanning detector reciprocates vertically.

[0012] Preferably, the scanning detector is U-shaped, with its scanning end facing the reaction vessel. The U-shaped design allows the detector to partially surround the reaction vessel for scanning operations.

[0013] Preferably, the first transmission mechanism includes a first motor connected to a first base, a first lead screw connected to the first motor, a connecting plate threaded to the first lead screw, and a first guide rail connected to the first base and parallel to the first lead screw. The first guide rail is slidably connected to the connecting plate, and the connecting plate is connected to the scanning detector. The first lead screw is vertically oriented, and the first guide rail is fixedly mounted on the first base via a guide rail mounting seat. The first motor drives the first lead screw to rotate, causing the connecting plate to move up and down under the action of the first guide rail, thereby enabling the scanning detector to complete the up and down scanning operation.

[0014] Preferably, the support base has an opening on the side facing the scanning end of the scanning detector. The opening in the support base allows the reaction vessel to be observed externally and facilitates scanning operations when the scanning detector moves up and down.

[0015] Preferably, the automated analysis system for detecting cryoglobulinemia further includes a mixing device, which comprises a second base, a second motor connected to the second base, and a vertically arranged mixing loading frame driven by the second motor. The second motor is vertically arranged, and the mixing loading frame has a loading cavity for loading reaction cups, and the mixing operation is completed by rotation; the mixing loading frame is driven by the second motor, enabling the mixing device to perform eccentric vortex mixing operation.

[0016] Preferably, the automated analysis system for detecting cryoglobulinemia further includes a transfer device for transferring reaction cups. The transfer device includes a clamping device and a second transmission mechanism that allows the clamping device to move vertically and laterally. This transfer mechanism facilitates the movement of the reaction cups containing serum, allowing the reaction cups to be kept warm for a certain period of time before being transferred to a scanning detector for scanning detection, to a mixing device for eccentric vortex mixing, and finally to a heating or cooling chamber.

[0017] Preferably, the second transmission mechanism includes a first moving component and a second moving component that is pulsatorically connected to the first moving component, the second moving component being connected to the gripping device. The first and second moving components, along with the gripping device, allow for lateral and vertical movement to accommodate the need to grip and transfer reaction cups at different positions, thus supporting scanning detection, temperature control, and other requirements, and reducing the impact of manual operation on the testing process.

[0018] Preferably, the first moving component includes a first frame, a third motor mounted on the first frame, a first transmission wheel connected to the third motor, a second transmission wheel, a first transmission belt connecting the first transmission wheel and the second transmission wheel, and a second slide rail fixedly connected to the first frame and arranged laterally.

[0019] The second moving component includes a second frame that is slidably connected to a second slide rail, a fourth motor mounted on the second frame, a third drive wheel connected to the fourth motor, a fourth drive wheel, a second drive belt connecting the third drive wheel and the fourth drive wheel, and a third slide rail that is fixedly connected to the second frame and vertically arranged. The second frame is connected to the first drive belt.

[0020] The clamping device is slidably connected to the third slide rail and connected to the second transmission belt. The second transmission wheel is movably connected to the first frame; the fourth transmission wheel is movably connected to the second frame; wherein the first and second transmission wheels are respectively located at the lateral ends of the first frame; the second slide rail and the first frame are both laterally arranged, and the direction of the second slide rail is parallel to the direction of movement of the upper end of the first transmission belt; along the frontal projection direction, the location of the second slide rail falls within the projection range of the first transmission belt; wherein the third motor is coaxially arranged with the first transmission wheel; wherein the third and fourth transmission wheels are respectively located at the vertical ends of the second frame; the third slide rail and the second frame are both vertically arranged, and the direction of the third slide rail is parallel to the direction of movement of one side of the second transmission belt; along the frontal projection direction, the location of the third slide rail is outside the projection plane of the second transmission belt; the fourth motor is coaxially arranged with the third transmission wheel; the third motor drives the first transmission wheel, the second transmission wheel, and the first transmission belt, causing the second frame connected to the first transmission belt to... The device can move along the first transmission belt, and the movement distance of the second frame is controlled by the third motor. A second slide rail limits the movement of the second frame to horizontal, allowing the gripping device to move horizontally under the action of the third motor. Simultaneously, a fourth motor drives the third and fourth transmission wheels and the second transmission belt, allowing the gripping device connected to the second transmission belt to move along it. The movement distance of the gripping device is controlled by the fourth motor, and the third slide rail limits the movement of the gripping device to vertical, allowing it to move vertically under the action of the fourth motor. This enables the gripping device to move both vertically and horizontally to meet the needs of gripping and transferring reaction cups at different positions, facilitating scanning detection, temperature preservation, and other requirements, while reducing the impact of manual operation on the testing process.

[0021] Preferably, the gripping device includes a third frame connected to the second transmission belt and a robotic arm connected to the third frame. The third frame is slidably connected to a third slide rail; a fourth motor drives the second transmission belt to move, allowing the third frame connected to the second transmission belt to move vertically, thereby moving the robotic arm.

[0022] Preferably, the robotic arm is vertically positioned, and an extended gripping arm is provided at its end. One end of the robotic arm is fixedly connected to the lower end of the third frame. The extended gripping arm allows the gripping device to reach deeper into the heating and cooling chambers to grasp the corresponding reaction cups.

[0023] Preferably, the first sensing modules are arranged in groups at both ends of the lower part of the first frame, and the second frame is provided with a first baffle; the movement path of the first baffle intersects with the sensing position of the first sensing module. This limits the maximum travel of the second frame at both ends, improving the installation performance during movement.

[0024] Preferably, a second sensing module is provided on one side of the second frame, and a second baffle is provided on the third frame; the movement path of the second baffle intersects with the sensing position of the second sensing module, thereby limiting the maximum vertical travel of the third frame.

[0025] Preferably, the automated analysis system for detecting cryoglobulinemia further includes a refrigeration chamber and / or a heating chamber for sample preservation. The refrigeration chamber is set at 4°C for sample preservation; the heating chamber is set at a constant temperature of 37°C for sample preservation. This configuration meets the requirement of maintaining a specific temperature for a certain duration during the experiment, satisfying the needs of the experimental comparison process. This setup offers high repeatability and controllability, reducing the impact of manual operation on the detection experiment.

[0026] Preferably, the cooling chamber is provided with a first cavity for accommodating the reaction cup; the heating chamber is provided with a second cavity for accommodating the reaction cup. This allows the reaction cup to be accommodated in both the cooling and heating chambers, meeting the requirement of preserving and reacting samples in the heating and cooling chambers at a certain temperature.

[0027] Preferably, the cooling chamber includes a cooling plate, a semiconductor cooling chip disposed on the cooling plate, a first heat sink, a fan disposed to the side of the first heat sink, and a first rotating disk for carrying the reaction vessel; the first cavity is disposed within the cooling plate, and the first rotating disk is disposed within the first cavity. This allows the cooling plate to maintain a certain temperature, ensuring the sample in the reaction vessel retains its reaction properties and meets experimental requirements.

[0028] Preferably, the cooling plate is equipped with a temperature sensor to detect the temperature of the cooling plate.

[0029] Preferably, the cooling plate is connected to a fifth motor, which is driven by the first rotating plate. This allows the first rotating plate to rotate, thereby enabling the reaction cup to be moved to the corresponding location of the through-hole for easy gripping by the clamping device.

[0030] Preferably, the heating chamber includes a heating plate and a second rotating disk that supports the reaction cup. The second cavity is disposed within the heating plate, and the second rotating disk is disposed within the second cavity. This allows the heating plate to maintain a certain temperature, ensuring the sample in the reaction cup retains its reaction properties and meets experimental requirements.

[0031] Preferably, the heating plate is connected to a sixth motor, which is driven by the second rotating plate. This allows the second rotating plate to rotate, enabling the reaction cup to be moved to the corresponding location of the through-hole for easy gripping by the clamping device.

[0032] Both the cooling and heating chambers are equipped with covers, each with a through-hole for extending a clamping arm to grip the reaction cup. The through-hole in the heating chamber faces the scanning detection device, as does the through-hole in the cooling chamber. The through-holes are relatively small, allowing the extended clamping arm to pass through and grip the reaction cup. This design minimizes open space above the cooling and heating chambers, improving their insulation performance. Furthermore, the rotation of the first or second rotating disc allows the reaction cup to rotate to the vertically corresponding position of the through-hole, facilitating gripping and reducing the impact on other samples, thus improving the stability of the detection process.

[0033] This application has achieved beneficial technical effects:

[0034] This invention uses a scanning detector to move up and down, scanning the serum in the reaction vessel during the movement to determine whether the sample in the reaction vessel has cloud-like turbidity or precipitation. This scanning detection method is easy to implement, highly stable, and avoids the subjectivity and low efficiency problems caused by manual interpretation. At the same time, its movement path is controllable, which is conducive to the standardized output of results. Attached Figure Description

[0035] Figure 1 The diagram shown is a structural schematic of this utility model;

[0036] Figure 2 The diagram shown is a top view of the present invention.

[0037] Figure 3 The diagram shown is a front view of the structure of this utility model;

[0038] Figure 4 The figure shown is a side view of the present invention.

[0039] Figure 5 The diagram shown is a side view of the present invention.

[0040] Figure 6 The diagram shown is a top view of the structure of the concealed housing of this utility model.

[0041] Figure 7 The figure shown is a three-dimensional structural schematic diagram of this utility model;

[0042] Figure 8The diagram shows the arrangement of the refrigeration chamber and the heating chamber of this utility model.

[0043] Figure 9 The diagram shown is a cross-sectional view of the present invention.

[0044] Figure 10 The figure shown is another three-dimensional structural schematic diagram of this utility model;

[0045] Figure 11 As shown Figure 10 A partial structural diagram;

[0046] Figure 12 The diagram shown is a structural schematic of the refrigeration chamber;

[0047] Figure 13 The diagram shown is a schematic representation of the scanning detection device.

[0048] Figure 14 The diagram shows a flowchart of the cryoglobulin assay method.

[0049] Figure Labels

[0050] 11-First base; 12-First transmission mechanism; 3-Reaction cup; 13-Bearing seat; 14-Scanning detector; 121-First motor; 122-First lead screw; 123-Connecting plate; 124-First guide rail; 125-Guide rail mounting plate; 131-Opening; 133-Optical coupler; 2-Mixing device; 21-Second base; 22-Second motor; 23-Mixing loading frame; 4-Transfer device; 41-Clamping device; 42-Second transmission mechanism; 43-First moving component; 44-Second moving component; 431-First frame; 432-Third motor; 433-First transmission wheel; 434-Second transmission wheel; 435-First transmission belt; 436-Second slide rail; 441-Second frame; 442-Fourth motor; 443-Third transmission wheel; 444-Fourth transmission wheel; 445-Second transmission belt; 446-Third slide rail; 411-Third frame; 412-Mechanical arm; 413-Extended gripper arm; 437-First sensing module; 447-First baffle; 448-Second sensing module; 414-Second baffle; 5-Refrigeration chamber; 6-Heating chamber; 50-First cavity; 60-Second cavity; 51-Refrigeration plate; 52-Semiconductor cooling chip; 53-First radiator; 54-Fan; 55-First rotating disk; 56-Temperature sensor; 57-Fifth motor; 61-Heating plate; 62-Second rotating disk; 63-Sixth motor. Detailed Implementation

[0051] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the specific implementation methods of this utility model will be described below with reference to the accompanying drawings. Obviously, the drawings described below are merely some embodiments of this utility model. For those skilled in the art, other drawings and other implementation methods can be obtained based on these drawings without any creative effort.

[0052] The technical solution of this utility model will be described in detail below with specific embodiments.

[0053] Reference Figures 1 to 14 As shown, an automated analytical system for detecting cryoglobulinemia includes a scanning detection device. The scanning detection device includes a first base 11, a first transmission mechanism 12 mounted on the first base 11, a support 13 mounted on the first base 11 to hold a reaction cup 3, and a scanning detector 14 that is connected to the first transmission mechanism 12 to perform reciprocating motion to scan the reaction cup 3. Specifically, the scanning detector 14 operates by moving up and down to scan. By moving the scanning detector 14 up and down and scanning the serum in the reaction cup 3 during this process, it can determine whether the sample in the reaction cup 3 has cloudy turbidity or precipitation. This scanning detection method is easy to implement, highly stable, and avoids the subjectivity and low efficiency problems caused by manual interpretation. Furthermore, its movement path is controllable, which is conducive to standardized output of results. The test position is used to test whether the sample has cloudy turbidity or precipitation, using up-and-down scanning detection.

[0054] The reaction cup 3 is vertically positioned, and the scanning detector 14 reciprocates vertically.

[0055] The scanning detector 14 is U-shaped, with its scanning end facing the reaction vessel 3. The U-shaped design of the scanning detector 14 allows it to partially surround the reaction vessel 3 for scanning operations.

[0056] The first transmission mechanism 12 includes a first motor 121 connected to the first base 11, a first lead screw 122 connected to the first motor 121, a connecting plate 123 threaded to the first lead screw 122, and a first guide rail 124 connected to the first base 11 and arranged parallel to the first lead screw 122. The first guide rail 124 is slidably connected to the connecting plate 123, and the connecting plate 123 is connected to the scanning detector 14. The scanning detector consists of two parts: one end emits light intensity, and the other end receives light intensity, converting the light signal into an electrical signal. The first lead screw 122 is vertically arranged, and the first guide rail 124 is fixedly mounted on the first base 11 via a guide rail mounting seat 125. The first motor 121 drives the first lead screw 122 to rotate, causing the connecting plate 123 to move up and down under the action of the first guide rail 124, thereby enabling the scanning detector 14 to complete the up and down scanning operation.

[0057] The support 13 has an opening 131 on the side facing the scanning end of the scanning detector 14. The opening 131 on the support allows the reaction cup 3 to be observed from the outside through the opening 131, and facilitates the scanning operation when the scanning detector 14 moves up and down;

[0058] The support 13 is provided with a first bracket 132 fixed to the first base 11. The first bracket 132 is provided with an optocoupler 133 on the side facing the connecting plate 123. The optocoupler 133 can limit the initial position and reset state of the scanning detector 14 driven by the first motor 121 to move up and down.

[0059] The automated analysis system for detecting cryoglobulinemia also includes a mixing device 2, which comprises a second base 21, a second motor 22 connected to the second base 21, and a vertically arranged mixing loading frame 23 driven by the second motor 22. The second motor 22 is vertically arranged, and the mixing loading frame 23 has a loading cavity 24 for loading reaction cups. Mixing is performed by rotation. The second motor 22 drives the mixing loading frame 23, enabling the mixing device 2 to perform eccentric vortex mixing. The mixing position is used for sample mixing, employing an eccentric vortex mixing method.

[0060] This technical solution also includes a wool roller brush 16; since there will be condensation on the wall of the reaction cup after cooling, the wool roller brush 16 is used to brush the condensation on the surface of the reaction cup 3.

[0061] The automated analysis system for detecting cryoglobulinemia also includes a transfer device 4 for transferring reaction cups. The transfer device 4 includes a clamping device 41 and a second transmission mechanism 42 that allows the clamping device 41 to move vertically and laterally. The transfer mechanism 4 facilitates the movement of the reaction cup 3 containing serum, allowing the reaction cup 3 to be kept warm for a certain period of time depending on different conditions, then transferred to the scanning detector 14 for scanning detection, to the mixing device 2 for eccentric vortex mixing, and finally moved and transferred to a heating chamber or a cooling chamber.

[0062] The second transmission mechanism 42 includes a first moving component 43 and a second moving component 44 that is drively connected to the first moving component 43. The second moving component 44 is connected to the clamping device 41. The first moving component 43 and the second moving component 44 are configured to allow the clamping device 41 to move laterally and vertically to meet the needs of clamping and transferring the reaction cup 3 at different positions, thus facilitating scanning detection, heat preservation, and other requirements, and reducing the impact of manual operation on the testing process.

[0063] The first moving component 43 includes a first frame 431, a third motor 432 mounted on the first frame 431, a first transmission wheel 433 connected to the third motor 432, a second transmission wheel 434, a first transmission belt 435 connecting the first transmission wheel 433 and the second transmission wheel 434, and a second slide rail 436 fixedly connected to the first frame 431 and arranged laterally.

[0064] The second moving component 44 includes a second frame 441 slidably connected to a second slide rail 436, a fourth motor 442 disposed on the second frame 441, a third transmission wheel 443 connected to the fourth motor 442, a fourth transmission wheel 444, a second transmission belt 445 connecting the third transmission wheel 443 and the fourth transmission wheel 444, and a third slide rail 446 fixedly connected to the second frame 441 and vertically disposed thereon. The second frame 441 is connected to the first transmission belt 435.

[0065] The clamping device 41 is slidably connected to the third slide rail 446, and the clamping device 41 is connected to the second transmission belt 445. The second transmission wheel 434 is movably connected to the first frame 431; the fourth transmission wheel 444 is movably connected to the second frame 441; wherein the first transmission wheel 433 and the second transmission wheel 434 are respectively set at the two transverse ends of the first frame 431; the second slide rail 436 and the first frame 431 are both transversely arranged, and the setting direction of the second slide rail 436 is parallel to the moving direction of the upper end of the first transmission belt 435; along the frontal projection direction, the setting position of the second slide rail 436 falls within the projection range of the first transmission belt 435; wherein the third motor 432 is coaxially set with the first transmission wheel 433. The third transmission wheel 443 and the fourth transmission wheel 444 are respectively set at the vertical ends of the second frame 441; the third slide rail 446 is vertically set along the second frame 441, and the setting direction of the third slide rail 446 is parallel to the moving direction of one side of the second transmission belt 445; along the frontal projection direction, the setting position of the third slide rail 446 is outside the projection plane of the second transmission belt 445; the fourth motor 442 is coaxially set with the third transmission wheel 443; the third motor 442 drives the first transmission wheel 433, the second transmission wheel 434, and the first transmission belt 435, so that the first transmission wheel 443, the second transmission wheel 444, and the first transmission belt 435 move together with the second transmission belt 445. A second frame 441 connected by a transmission belt 435 can move following the first transmission belt 435. The movement distance of the second frame 441 is controlled by a third motor 432. A second slide rail 436 is provided to limit the movement direction of the second frame 441 to horizontal, allowing the clamping device 41 to move horizontally under the action of the third motor 432. Simultaneously, a fourth motor 442 is provided, which drives the third transmission wheel 443, the fourth transmission wheel 444, and the second transmission belt 445, causing the clamping device 41 connected to the second transmission belt 445 to move horizontally. The gripping device 41 can move along the second transmission belt 445, thereby controlling the movement distance of the gripping device 41 through the fourth motor 442. A third slide rail 446 is provided to limit the movement direction of the gripping device 41 to vertical, so that the gripping device 41 can move vertically under the action of the fourth motor 442. This allows the gripping device 41 to move vertically and horizontally to meet the needs of gripping and transferring the reaction cup 3 at different positions, in order to meet the needs of scanning detection, heat preservation, etc., and reduce the impact of manual operation on the detection test.

[0066] The gripping device 41 includes a third frame 411 connected to the second transmission belt 445 and a robotic arm 412 connected to the third frame 411. The third frame 411 is slidably connected to the third slide rail 446. The second transmission belt 445 is moved by the fourth motor 442, so that the third frame 411 connected to the second transmission belt 445 can move vertically, thereby driving the robotic arm 412 to move.

[0067] The robotic arm 412 is vertically positioned, and an extended gripping arm 413 is provided at its end. One end of the robotic arm 412 is fixedly connected to the lower end of the third frame 411. The extended gripping arm 413 allows the gripping device 41 to reach deeper, making it easier to extend into the heating chamber and cooling chamber to grip the corresponding reaction cups.

[0068] The first frame 431 has first sensing modules 437 arranged in groups at both ends below it, and the second frame 441 has a first baffle 447. The moving path of the first baffle 447 intersects with the sensing position of the first sensing module 437. This limits the maximum travel of the second frame 441 at both ends, improving the installation stability during movement.

[0069] A second sensing module 448 is provided on one side of the second frame 441, and a second baffle 414 is provided on the third frame 411; the moving path of the second baffle 414 intersects with the sensing position of the second sensing module 448, thereby limiting the maximum vertical travel of the third frame 441.

[0070] The automated analysis system for detecting cryoglobulinemia also includes a cooling chamber 5 and a heating chamber 6 for sample preservation. The cooling chamber 5 is set at a temperature of 4°C for sample preservation; the heating chamber 6 is set at a constant temperature of 37°C for sample preservation. This configuration meets the requirement of maintaining a certain temperature for a certain duration during the experiment, satisfying the needs of the experimental comparison process. This setup offers high repeatability and controllability, reducing the impact of manual operation on the detection experiment.

[0071] The cooling chamber 5 is provided with a first cavity 50 for accommodating the reaction cup 3; the heating chamber 6 is provided with a second cavity 60 for accommodating the reaction cup 3. This allows the reaction cup 3 to be accommodated in both the cooling chamber 5 and the heating chamber 6, meeting the requirement that the samples in the heating chamber 6 and the cooling chamber 5 be preserved and reacted at a certain temperature.

[0072] The cooling chamber 5 includes a cooling plate 51, a semiconductor cooling chip 52 disposed on the cooling plate 51, a first heat sink 53, a fan 54 disposed on the side of the first heat sink 53, and a first rotating disk 55 supporting the reaction cup 3; the first cavity 50 is disposed within the cooling plate 51, and the first rotating disk 55 is disposed within the first cavity 50. This allows the cooling plate 51 to maintain a certain temperature, ensuring the sample in the reaction cup 3 is preserved and reacted, thus meeting experimental requirements.

[0073] The cooling plate 51 is equipped with a temperature sensor 56 to detect the temperature of the cooling plate 51.

[0074] The cooling plate 51 is connected to the fifth motor 57, which is in turn connected to the first rotating plate 55. This allows the first rotating plate 55 to rotate, thereby enabling the reaction cup 3 to be moved to the corresponding location of the through-hole for easy gripping by the clamping device 41.

[0075] The heating chamber 6 includes a heating plate 61 and a second rotating disk 62 that supports the reaction cup 3. The second cavity 60 is disposed within the heating plate 61, and the second rotating disk 62 is disposed within the second cavity 60. This allows the heating plate 61 to maintain a certain temperature, ensuring the sample in the reaction cup 3 is preserved and reacted, thus meeting experimental requirements.

[0076] The heating plate 61 is connected to the sixth motor 63, which is driven by the second rotating plate 62. This allows the second rotating plate 62 to rotate, thereby enabling the reaction cup 3 to be moved to the corresponding location of the through-hole for easy gripping by the clamping device 41.

[0077] Both the cooling chamber 5 and the heating chamber 6 are equipped with covers 64. The covers 64 have through openings 641 for extending the clamping arm 413 to grip the reaction cup 3. The through opening 641 of the heating chamber is located on the side facing the scanning detection device, and the through opening 641 of the cooling chamber 5 is located on the side facing the scanning detection device. The through openings 641 are relatively small, allowing the extended clamping arm 413 to pass through and grip the reaction cup 3. This results in less open space above the cooling chamber 5 and the heating chamber 6 that is connected to the outside, improving the heat preservation performance of the heating chamber 6 and the cooling chamber 5. At the same time, the reaction cup 3 is rotated to the vertical position corresponding to the through opening 641 by the rotation of the first rotating disk 55 or the second rotating disk 62, which facilitates the gripping operation and reduces the impact on other samples, thus improving the stability of the detection process.

[0078] A method for operating an automated analytical system for detecting cryoglobulinemia, characterized by comprising the following steps:

[0079] S100, the scanning detector moves up and down and scans the serum loaded in the reaction vessel;

[0080] S200 determines whether the serum in the reaction cup appears cloudy or precipitated based on the scanning results of the scanning detector.

[0081] S300 compares the results before and after the initial placement of the reaction vessel and after a certain reaction time, and outputs the results.

[0082] In S200, the judgment is made based on different electrophoretic peaks, specifically by comparing the light intensity and peak curves before and after the reaction.

[0083] In S300, when the reaction cup is initially placed, the scanning detector performs a round of scanning test data; after a certain reaction time, another round of scanning test data is performed, and the two sets of data are compared according to the database type to determine whether the sample is positive or negative for cryoglobulin.

[0084] Meanwhile, the samples exhibit differences in other trace elements, such as differences in the types of patients with jaundice or blood sugar. Different peak curve types obtained based on these differences can be compared with the database to achieve cryoglobulin typing.

[0085] The step S1001 is preceding step S100.

[0086] S1001, extract the serum, divide it into 2 portions, each 1 ml, and seal them in reaction cups. Place the two reaction cups containing the serum in a refrigeration chamber, where the temperature is 4℃ and the temperature is maintained for 24 hours.

[0087] The reaction cups were initially placed with two serum samples in a refrigerated chamber for 24-72 hours. The serum in the reaction cups was scanned and tested every 24 hours to determine whether there was a change in the turbidity of the sample, which would appear as a cloudy or precipitated substance.

[0088] Specifically, after the reaction vessel reacts for a certain period of time, the reaction process is as follows:

[0089] If both serum samples show cloudy turbidity or precipitation within 24 to 72 hours, one of the serum samples should be vortexed and placed in a heating chamber for 3 hours for scanning and testing. If the turbidity or precipitation disappears, the sample is confirmed to be positive for cryoglobulin. If the turbidity or precipitation does not disappear, an additional judgment procedure is required. The system reports a suspicious result, requiring further separation and identification.

[0090] If the two samples show inconsistency within 24 hours to 72 hours, the sample will be vortexed and placed in a heating chamber for 3 hours after cloudiness or precipitation. The sample will then be scanned and tested. If the turbidity or precipitation disappears, the sample is confirmed to be positive for cryoglobulin. If the turbidity or precipitation does not disappear, the sample is confirmed to be negative for cryoglobulin.

[0091] If no cloudy turbidity or precipitation appears in either serum sample within 24 to 72 hours, continue to place them for one week. If no precipitation still appears, the sample is confirmed to be cryoglobulin negative. If cloudy turbidity or precipitation appears in the scan after one week, vortex and place at 35°C to 40°C for 3 hours. When the turbidity or precipitation disappears in the scan, the sample is confirmed to be cryoglobulin positive. If the turbidity or precipitation does not disappear, proceed to the additional judgment procedure; the system reports the result as suspicious and requires further separation and identification.

[0092] The temperature inside the heating chamber is maintained at 37°C; the temperature inside the cooling chamber is maintained at 4°C, and four semiconductor cooling plates are used for cooling; a gap is set between the cooling plates and the heating plates; the reaction cup is transparent to facilitate scanning operations; both the cooling chamber 5 and the heating chamber 6 are used for sample preservation during the reaction.

[0093] The analysis system of this technical solution sets the process flow, judgment method, and sample placement time according to the detection method;

[0094] The scanning measurement device consists of a reaction cup, a reaction cup holder, a scanning detector, a support, an optocoupler, a guide rail mounting base, a guide rail, a connecting plate, a base, and a lead screw motor. The reaction cup is placed on the reaction cup holder, and the lead screw motor drives the scanning detector up and down via the connecting plate and the guide rail. The scanning detector is U-shaped, emitting a light source on one side and receiving the light source brightness on the other, determining whether the reaction solution in the reaction cup is turbid. The scanning detector performs one round of scanning tests immediately after the reaction cup is placed, and another round of scanning tests after the reaction. The two sets of data are compared according to the database type to analyze whether the patient has cryoglobulinemia.

[0095] Currently, the detection of cryoglobulinemia mainly relies on medical staff visually determining whether a result is positive or negative. Due to the lack of specific standards for different types of samples, individual differences exist in the interpretation of test results by different medical staff, which undoubtedly affects the accuracy and consistency of the test results.

[0096] To change this situation, introducing instrument-assisted manual procedures for cryoglobulinemia can effectively free up the hands of medical staff and save them time and energy. Developing an intelligent electrophoresis peak analysis method is urgently needed. This method should be unaffected by human factors, capable of quickly and accurately classifying different electrophoresis peaks and outputting standardized interpretations. This is of great significance for improving the efficiency of electrophoresis result interpretation and ensuring its completeness, objectivity, and consistency.

[0097] In practice, serum cryoglobulin analysis in laboratories is time-consuming and struggles to obtain optimal sampling conditions. Furthermore, even cryoglobulins at significantly low levels can trigger severe symptoms in some patients, a fact that has not received sufficient attention. Inappropriate sample handling is a key factor affecting the standardization of cryoglobulin detection. For a long time, the quantification and typing of cryoglobulins has been a major challenge for clinical laboratories. Result interpretation and calculation rely excessively on manual processes, making them susceptible to both endogenous and exogenous interference factors, and also suffer from high subjectivity, low efficiency, high dependence on specialized knowledge, and sensitivity to specific conditions. In addition, the output of results lacks complete and standardized interpretation. Therefore, developing the aforementioned intelligent electrophoresis peak analysis method is of significant practical importance for improving clinical testing levels and is a key measure to promote technological innovation in cryoglobulin detection. This technical solution also includes a data processing system for data comparison based on database type.

[0098] This technical solution also includes a housing, on which a display screen is provided for operating and observing the instrument's status.

[0099] This utility model discloses an analytical system for the automated detection of cryoglobulinemia, the key component of which is a scanning detection device. This scanning detection device mainly includes a first base on which a first transmission mechanism is mounted; a carrier is also mounted on the first base for holding reaction cups. Notably, the scanning detector is driven by the first transmission mechanism, enabling the scanning detector to perform precise reciprocating motion, thereby conducting a comprehensive scan of the reaction cups on the carrier.

[0100] During operation, the scanning detector moves up and down along a specific path, meticulously scanning the serum within the reaction vessel to determine if any cloudy turbidity or precipitation occurs. Compared to traditional manual interpretation, this scanning detection method offers significant advantages: First, it is less complex to implement, with a simple structural design and operating principle, making it easy to manufacture and install. Second, it boasts extremely high stability; the mechanical transmission combined with the precise scanning instrument effectively reduces interference from external factors. Third, it successfully avoids the inherent subjectivity of manual interpretation, resulting in more objective and impartial test results. Fourth, it significantly improves detection efficiency, enabling the testing of a large number of samples in a short time. Finally, the precisely controllable movement path of the scanning detector provides strong support for the standardized output of test results, facilitating subsequent data statistics and analysis, and making the test results more universal and comparable.

[0101] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0102] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

[0103] The embodiments of the automated analytical system for detecting cryoglobulinemia provided by this utility model have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of this utility model. The descriptions of the embodiments above are only for the purpose of helping to understand the core ideas of this utility model. It should be noted that those skilled in the art can make several improvements and modifications to this utility model without departing from the principles of this utility model, and these improvements and modifications also fall within the protection scope of the claims of this utility model.

Claims

1. An analytical system for automated detection of cryoglobulinemia, characterized by, The device includes a scanning detection device, which includes a first base (11), a first transmission mechanism (12) disposed on the first base (11), a support seat (13) disposed on the first base (11) to load the reaction cup (3), and a scanning detector (14) that is connected to the first transmission mechanism (12) to perform vertical reciprocating motion to scan the reaction cup (3). The reaction cup (3) is arranged vertically.

2. The analytical system for automated detection of cryoglobulinemia according to claim 1, characterized in that, The scanning detector (14) is U-shaped, and the scanning end of the scanning detector (14) is positioned facing the reaction cup (3).

3. The analytical system for automated detection of cryoglobulinemia according to claim 1, characterized in that, The first transmission mechanism (12) includes a first motor (121) connected to the first base (11), a first lead screw (122) connected to the first motor (121), a connecting plate (123) threaded to the first lead screw (122), and a first guide rail (124) connected to the first base (11) and arranged parallel to the first lead screw (122). The first guide rail (124) is slidably connected to the connecting plate (123), and the connecting plate (123) is connected to the scanning detector (14).

4. The analytical system for automated detection of cryoglobulinemia according to claim 1, characterized in that, The automated analysis system for detecting cryoglobulinemia also includes a mixing device (2), which includes a second base (21), a second motor (22) connected to the second base (21), and a mixing loading frame (23) that is driven by the second motor (22) and is vertically arranged.

5. The analytical system for automated detection of cryoglobulinemia according to claim 1, characterized in that, The automated analysis system for detecting cryoglobulinemia also includes a transfer device (4) for transferring reaction cups, the transfer device (4) including a clamping device (41) and a second transmission mechanism (42) that allows the clamping device (41) to move vertically and laterally.

6. The analytical system for automated detection of cryoglobulinemia according to claim 5, characterized in that, The second transmission mechanism (42) includes a first moving component (43) and a second moving component (44) that is connected to the first moving component (43) in a transmission manner. The second moving component (44) is connected to the clamping device (41). The first moving component (43) includes a first frame (431), a third motor (432) mounted on the first frame (431), a first transmission wheel (433) connected to the third motor (432), a second transmission wheel (434), a first transmission belt (435) connecting the first transmission wheel (433) and the second transmission wheel (434), and a second slide rail (436) fixedly connected to the first frame (431) and arranged laterally. The second moving component (44) includes a second frame (441) slidably connected to a second slide rail (436), a fourth motor (442) mounted on the second frame (441), a third drive wheel (443) connected to the fourth motor (442), a fourth drive wheel (444), a second drive belt (445) connecting the third drive wheel (443) and the fourth drive wheel (444), and a third slide rail (446) fixedly connected to the second frame (441) and vertically arranged. The second frame (441) is connected to the first drive belt (435). The clamping device (41) is slidably connected to the third slide rail (446), and the clamping device (41) is connected to the second transmission belt (445).

7. The analytical system for automated detection of cryoglobulinemia according to claim 1, characterized in that, The automated analytical system for detecting cryoglobulinemia also includes a refrigeration chamber (5) and / or a heating chamber (6) for sample preservation reactions.

8. The analytical system for automated detection of cryoglobulinemia according to claim 7, characterized in that, The refrigeration chamber (5) is provided with a first cavity (50) for accommodating the reaction cup (3); the heating chamber (6) is provided with a second cavity (60) for accommodating the reaction cup (3).

9. The analytical system for automated detection of cryoglobulinemia according to claim 8, characterized in that, The refrigeration chamber (5) includes a refrigeration plate (51), a semiconductor refrigeration chip (52) disposed on the refrigeration plate (51), a first heat sink (53), a fan (54) disposed on the side of the first heat sink (53), and a first rotating disk (55) that carries the reaction cup (3); the first cavity (50) is disposed in the refrigeration plate (51), and the first rotating disk (55) is disposed in the first cavity (50); the refrigeration plate (51) is connected to a fifth motor (57), and the fifth motor (57) is drivenly connected to the first rotating disk (55).

10. The analytical system for automated detection of cryoglobulinemia according to claim 8, characterized in that, The heating chamber (6) includes a heating plate (61) and a second rotating disk (62) that carries the reaction cup (3). The second cavity (60) is disposed in the heating plate (61) and the second rotating disk (62) is disposed in the second cavity (60). The heating plate (61) is connected to a sixth motor (63), and the sixth motor (63) is connected to the second rotating disk (62) in a transmission connection.

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