A pathological tissue surface decalcification box

Abstract

This invention provides a decalcification box for pathological tissue surfaces, belonging to the field of pathological technology. It includes a box body and a lid. The bottom of the box body has parallel grooves and raised ridges, with multiple protrusions on the raised ridges. This invention achieves zero power dependence, low manufacturing cost, stable and robust structure, and good applicability. The grooves form capillary drainage channels, allowing the decalcification solution to automatically diffuse to the entire surface of the paraffin block, reducing the frequency of manual shaking and making the decalcification solution flow more actively. This ensures more thorough contact between the paraffin block surface and the decalcification solution, improving decalcification efficiency. The protrusions lift the paraffin block off the bottom of the box, ensuring that the surfaces of paraffin blocks of different sizes are fully exposed to the decalcification solution, further enhancing contact. The distance between the paraffin block and the bottom of the box ensures free circulation of the decalcification solution, preventing localized low decalcification efficiency.

Description

Technical Field

[0001] This utility model belongs to the field of pathology technology and relates to a decalcification box for the surface of pathological tissues. Background Technology

[0002] Some surgical lesions may contain calcification, while others are bone tissue. These specimens are sent to the pathology department, where they undergo acid immersion and decalcification during sampling. During pathological tissue processing, calcified or bone tissue specimens must be decalcified before subsequent sectioning and staining. Currently, the mainstream methods include:

[0003] 1. Traditional whole-tissue decalcification method: The specimen is immersed entirely in an acidic decalcification solution (such as hydrochloric acid, formic acid, etc.), and the decalcification endpoint is determined by a needle prick test to check the hardness. This method takes approximately 2-72 hours. Although widely used, this method has significant drawbacks:

[0004] Severe tissue damage: Prolonged penetration of strong acid leads to destruction of antigenic epitopes, degradation of nucleic acids, and deformation of cell morphology;

[0005] Distortion of test results: The false negative rate of subsequent immunohistochemical (IHC) staining increases, the quality of nucleic acid extraction decreases, and the accuracy of molecular pathological diagnosis is affected.

[0006] 2. Surface decalcification method: To overcome the shortcomings of traditional methods, improved processes have been proposed in recent years (such as the research published by the applicant's team in the Chinese Journal of Pathology in 2020):

[0007] First, the tissue is dehydrated and embedded to form a wax block, and then the exposed cut surface is roughly trimmed.

[0008] The cut surface of the wax block is horizontally immersed in the decalcification solution (the liquid surface covers about 0.3 cm), and then treated with 80% ethanol for acid removal.

[0009] This method only softens the surface tissue and significantly shortens the acid exposure time. Literature confirms that it can improve IHC staining intensity and nucleic acid integrity (supported by about 10 related studies in China).

[0010] However, the following problems still exist:

[0011] Lack of dedicated decalcification containers: Currently, laboratories mostly use recycled plastic slide boxes as a substitute, which has the following drawbacks:

[0012] Uneven penetration of decalcification solution: The cut surface of the wax block is in direct contact with the smooth bottom of the box, forming a sealed gap that hinders the diffusion of the liquid, requiring frequent manual shaking intervention;

[0013] Significant reagent waste: Containers lack liquid level markings, making it easy to add excessive amounts of expensive decalcification solution;

[0014] Environmental pollution risk: The open design causes acid to evaporate, releasing irritating odors and endangering the health of operators.

[0015] The existing patent document with authorization publication number CN221883228U describes a tissue paraffin block surface decalcification instrument, but it relies on electric drive and integrates complex modules such as temperature control / ultrasound, resulting in a large size, high cost, and complicated operation, making it difficult to popularize in primary pathology departments.

[0016] In summary, while surface decalcification has technological advantages, its large-scale clinical application is severely limited by the lack of suitable containers and the over-engineering of existing equipment. There is an urgent need to design a simple, low-cost, and standardized dedicated decalcification container to achieve efficient and controllable surface decalcification operations. Utility Model Content

[0017] To address the shortcomings of existing technologies, the purpose of this invention is to provide a decalcification box for the surface of pathological tissues, thereby solving the problems mentioned in the background art. This invention features several protrusions and grooves, with the grooves forming capillary drainage channels. The decalcification solution automatically diffuses to the entire area of ​​the paraffin block cut surface, reducing the frequency of manual shaking and making the decalcification solution flow more actively, thus ensuring more thorough contact between the paraffin block cut surface and the decalcification solution.

[0018] To achieve the above objectives, this utility model is implemented through the following technical solution:

[0019] A decalcification box for pathological tissue surfaces includes a box body and a box lid. The bottom of the box body is provided with parallel grooves and protrusions, and a plurality of protrusions are provided on the protrusions.

[0020] Furthermore, the multiple protrusions include fan-shaped protrusions located at the four corners of the box body, and cylindrical protrusions distributed at the bottom of the box body, wherein the top surface of the cylindrical protrusions is provided with open longitudinal and transverse lines.

[0021] Furthermore, a drainage hole is provided at one corner of the box cover.

[0022] Furthermore, the groove has a depth of 1 mm, a width of 1.5 mm, and a spacing of 2 mm between adjacent grooves.

[0023] Furthermore, the radius of the fan-shaped protrusion is 3mm, the radius of the cylindrical protrusion is 2mm, and the height of both the fan-shaped and cylindrical protrusions is 1.5mm.

[0024] Furthermore, the box body and the lid are hinged together, the upper edge of the box body is provided with a recessed area, and the front end of the lid is provided with a buckle that matches the recessed area to achieve a tight seal.

[0025] Furthermore, the drainage hole is an equilateral right triangle structure with a side length of 8mm.

[0026] Furthermore, the dimensions of the box body are 9.0cm in length, 7.0cm in width, and 3cm in height, and the dimensions of the box lid are 9.0cm in length, 7.0cm in width, and 1cm in height.

[0027] Furthermore, the inner wall of the box is provided with liquid level markings.

[0028] Furthermore, the scale line is provided with a marking line, and the vertical height difference between the marking line and the highest point of the protrusion is 3mm.

[0029] The beneficial effects of this utility model are:

[0030] Compared with the prior art, this utility model achieves zero power dependence, low manufacturing cost, stable and robust structure, and good applicability;

[0031] This invention features multiple protrusions and grooves, with the grooves forming capillary drainage channels. The decalcification solution automatically diffuses to the entire area of ​​the wax block's cut surface, reducing the frequency of manual shaking and making the decalcification solution flow more actively. This allows for more thorough contact between the wax block's cut surface and the decalcification solution, thereby improving decalcification efficiency.

[0032] The raised section lifts the wax block off the bottom of the box, allowing the cut surfaces of wax blocks of different sizes to be fully exposed to the decalcification solution, resulting in more thorough contact between the cut surfaces of the wax blocks and the decalcification solution. The distance between the wax block and the bottom of the box ensures free circulation of the decalcification solution, preventing localized low decalcification efficiency or failure.

[0033] This invention, by setting a marking line, with a height difference of 3mm between the marking line on the inner side wall of the box and the highest point of the protrusion, allows for strict control of the tissue immersion depth at around 3mm when injecting liquid according to the marking line. This effectively avoids waste of reagents and ensures a high degree of consistency between the results of novice operations and those of experienced technicians, thus mitigating human error. Attached Figure Description

[0034] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0035] Figure 1 This is a structural schematic diagram of an embodiment of the present utility model;

[0036] Figure 2 This is a top view of the box body according to an embodiment of the present utility model;

[0037] Figure 3 This is a cross-sectional view of the box body according to an embodiment of the present utility model;

[0038] Figure 4 This is a cross-sectional view of the box lid according to an embodiment of the present utility model;

[0039] Figure 5 This is an embodiment of the present utility model. Figure 2Enlarged view of point A in the middle.

[0040] In the diagram: 1. Box body; 2. Box lid; 3. Groove; 4. Scale line; 5. Drain hole; 6. Fan-shaped protrusion; 7. Cylindrical protrusion; 8. Recessed area; 9. Buckle; 10. Raised ridge; 11. Marking line. Detailed Implementation

[0041] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0042] like Figure 1-2 As shown, a decalcification box for pathological tissue surfaces includes a box body 1 and a box lid 2. The bottom of the box body 1 is provided with parallel grooves 3 and protrusions 10. Several protrusions 10 are provided with multiple protrusions, including fan-shaped protrusions 6 located at the four corners of the box body 1 and cylindrical protrusions 7 distributed at the bottom of the box body 1. The top surface of the cylindrical protrusions 7 is provided with open longitudinal and transverse grooves, the grooves are 0.5mm deep and play a certain drainage role. The longitudinal and transverse grooves play an anti-slip role for the wax block placed on the top of the cylindrical protrusions 7. The grooves 3 are 1mm deep and 1.5mm wide, and the distance between adjacent grooves 3 is 2mm. The top surface of the cylindrical protrusions 7 is provided with anti-slip grooves. The radius of the fan-shaped protrusions 6 is 3mm, the radius of the cylindrical protrusions 7 is 2mm, and the height of the fan-shaped protrusions 6 and the cylindrical protrusions 7 is 1.5mm.

[0043] The groove 3 forms a capillary drainage channel, and the decalcification solution automatically diffuses to the entire area of ​​the wax block cut surface, reducing the frequency of manual shaking and making the decalcification solution flow more actively. The protrusion lifts the wax block away from the bottom of the box, so that the cut surfaces of wax blocks of different sizes can be fully exposed to the decalcification solution, making the wax block cut surface and the decalcification solution more fully in contact. The distance between the wax block and the bottom of the box ensures the free circulation of the decalcification solution and prevents local decalcification failure.

[0044] The inner wall of the box 1 is provided with a liquid level height scale line 4, and the scale line 4 is provided with a marking line 11. The vertical height difference between the marking line 11 and the highest point of the protrusion is 3mm.

[0045] The height difference between the marking line 11 on the inner side wall of the box 1 and the highest point of the protrusion is 3mm. When injecting liquid according to the marking line 11, the tissue immersion depth can be strictly controlled at about 3mm, which can effectively avoid the waste of reagents.

[0046] like Figure 1-4As shown, the lid 2 has a drainage hole 5 at one corner. The drainage hole 5 is an equilateral right triangle with a side length of 8mm. The box body 1 is hinged to the lid 2. The upper edge of the box body 1 has a recessed area 8. The front end of the lid 2 has a buckle 9 that matches the recessed area 8 to achieve a tight seal. The dimensions of the box body 1 are 9.0cm long, 7.0cm wide, and 3cm high. The dimensions of the lid 2 are 9.0cm long, 7.0cm wide, and 1cm high.

[0047] The lid 2 and the body 1 are sealed and locked by the buckle 9, which prevents the acid from evaporating, maintains a constant concentration of decalcification solution, avoids the decrease in decalcification efficiency due to evaporation, and the airtight design reduces the concentration of irritating odor in the room, thus protecting the health of personnel.

[0048] The lid 2 is equipped with a right-angled triangular drainage hole 5, so that the waste liquid can be poured out without opening the lid. The acid liquid is directly discharged into the waste liquid tank through the drainage hole 5, and the operator has zero contact.

[0049] The specific operation method of this utility model is as follows:

[0050] Step 1: Place the wax block

[0051] Open the lid 2 and place the roughly trimmed tissue wax block, cut side down, inside the box 1. The wax block is stably supported by a combination of protrusions: the four corner fan-shaped protrusions 6 support the edge of the wax block; the bottom cylindrical protrusion 7 has anti-slip texture on the top surface to prevent displacement; the protrusion height is 1.5mm to ensure that the cut surface of the wax block forms a flow gap with the bottom of the box.

[0052] Step 2: Injection of decalcification solution

[0053] Inject decalcification solution into the box along the side wall of box 1; stop injecting solution when the liquid level reaches the mark line 11;

[0054] The decalcification solution automatically diffuses through the parallel groove 3 at the bottom, immersing the cut surface of the wax block to a depth of 3.0±0.2mm with precision.

[0055] Step 3: Closed-loop decalcification

[0056] Close the lid 2 and press the front buckle 9 of the lid 2 into the recessed area 8 of the box body 1 to achieve a seal and lock; let it stand for decalcification, the time depends on the degree of calcification of the specimen; the sealed environment prevents the volatilization of acid mist and maintains the pH value of the decalcification solution. During this period, the box body 1 can be shaken slightly to make the decalcification solution flow actively, but it will not affect the displacement of the wax block.

[0057] Step 4: Waste liquid discharge

[0058] Hold the edge of box 1 and tilt the decalcification box at 45°; the waste liquid flows into the waste liquid tank through the right-angled triangular drainage hole 5 of the box cover 2; the waste liquid can be discharged without opening the cover throughout the process.

[0059] This invention achieves zero power dependence, has low manufacturing cost, stable and robust structure, and good applicability;

[0060] By setting up several protrusions and several grooves 3, the grooves 3 form capillary drainage channels, and the decalcification liquid automatically diffuses to the entire area of ​​the wax block cut surface, reducing the frequency of manual shaking, making the decalcification liquid flow more actively, so that the wax block cut surface and the decalcification liquid are in more sufficient contact, thus improving the decalcification efficiency.

[0061] The raised section lifts the wax block off the bottom of the box, allowing the cut surfaces of wax blocks of different sizes to be fully exposed to the decalcification solution, resulting in more thorough contact between the cut surfaces of the wax blocks and the decalcification solution. The distance between the wax block and the bottom of the box ensures free circulation of the decalcification solution, preventing local decalcification failure.

[0062] By setting the marking line 11, the height difference between the marking line 11 on the inner side wall of the box body 1 and the highest point of the protrusion is 3mm. When injecting liquid according to the marking line 11, the tissue immersion depth can be strictly controlled at about 3mm, which can effectively avoid the waste of reagents and make the results of novice operation highly consistent with those of senior technicians, thus avoiding human error.

[0063] The lid 2 and the body 1 are sealed and locked by the buckle 9, which prevents the acid from evaporating, maintains a constant concentration of decalcification solution, avoids the decrease in decalcification efficiency due to evaporation, and the airtight design reduces the concentration of irritating odor in the room, thus protecting the health of personnel.

[0064] The lid 2 is equipped with a right-angled triangular drainage hole 5, so that the waste liquid can be poured out without opening the lid. The acid liquid is directly discharged into the waste liquid tank through the drainage hole 5, and the operator has zero contact.

[0065] Although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole. The technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A pathological tissue surface decalcification box comprising a box body (1) and a box cover (2), characterized in that: The bottom of the box body (1) is provided with parallel grooves (3) and protrusions (10), and a plurality of protrusions are provided on the protrusions (10), and the grooves (3) form capillary drainage channels.

2. The pathological tissue surface decalcification box according to claim 1, characterized in that: The multiple protrusions include fan-shaped protrusions (6) located at the four corners of the box body (1) and cylindrical protrusions (7) distributed at the bottom of the box body (1), the top surface of which is provided with open longitudinal and transverse lines.

3. The pathological tissue surface decalcification box according to claim 1, characterized in that: The lid (2) has a drainage hole (5) at one corner.

4. A decalcification box for pathological tissue surface according to claim 1, characterized in that: The groove (3) has a depth of 1 mm and a width of 1.5 mm, and the distance between adjacent grooves (3) is 2 mm.

5. A decalcification box for pathological tissue surface according to claim 2, characterized in that: The radius of the fan-shaped protrusion (6) is 3mm, the radius of the cylindrical protrusion (7) is 2mm, and the height of both the fan-shaped protrusion (6) and the cylindrical protrusion (7) is 1.5mm.

6. A decalcification box for pathological tissue surface according to claim 1, characterized in that: The box body (1) is hinged to the box cover (2). The upper edge of the box body (1) is provided with a recessed area (8), and the front end of the box cover (2) is provided with a buckle (9) that matches the recessed area (8) to achieve airtight locking.

7. A decalcification box for pathological tissue surface according to claim 3, characterized in that: The drainage hole (5) is an equilateral right triangle with a side length of 8mm.

8. A decalcification box for pathological tissue surface according to claim 1, characterized in that: The dimensions of the box body (1) are 9.0cm long, 7.0cm wide, and 3cm high, and the dimensions of the lid (2) are 9.0cm long, 7.0cm wide, and 1cm high.

9. A decalcification box for pathological tissue surface according to claim 1, characterized in that: The inner wall of the box (1) is provided with liquid level height scale lines (4).

10. A decalcification box for pathological tissue surface according to claim 9, characterized in that: The scale line (4) is provided with a marking line (11), and the vertical height difference between the marking line (11) and the highest point of the protrusion is 3mm.

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