A microfluidic chip device

Abstract

The utility model embodiment provides a kind of microfluidic chip device, comprising: by base and top plate are composed, oil storage chamber, magnetic bead storage chamber, polypeptide storage chamber, microdrop storage chamber, first mixing part and second mixing part are arranged in recessed inlay on the base, the first mixing part is equipped with first inlet and first outlet, the magnetic bead storage chamber with the polypeptide storage chamber is communicated by flow channel the first inlet, the oil storage chamber is communicated the first outlet by double flow channel intersection, the second mixing part is equipped with second inlet and second outlet, the second inlet is communicated the first outlet, the second outlet is communicated the microdrop storage chamber.The utility model simplifies chip structure, reduces manufacturing cost and assembly complexity, utilizes barrier column microflow design, and rotation mixing of magnetic bead polypeptide solution is realized by flow rate control, significantly improve the uniformity of magnetic bead and polypeptide mixing, while microfluidic system significantly reduces reagent consumption, reduces experimental cost.

Description

Technical Field

[0001] This utility model relates to the field of microfluidics, and in particular to a microfluidic chip device. Background Technology

[0002] Microfluidics is an interdisciplinary technology that precisely manipulates fluid flow at the micrometer scale. By integrating complex processes such as sample processing, reaction mixing, separation, and detection onto a chip of just a few square centimeters, it has demonstrated significant value in fields such as biomedical detection, chemical synthesis, and the fabrication of micro and nanomaterials. Typical microfluidic chips are constructed from polymers, silicon-based materials, or glass, with an internal network of microchannels ranging from 10 μm to 1 mm. However, existing microfluidic chips still face significant technical bottlenecks in applications involving multiphase mixing and microdroplet fabrication.

[0003] Current microfluidic channel designs often employ symmetrical diversion-convergence structures or simple bends. These configurations suffer from insufficient fluid dynamic control: First, the symmetrical structure causes the fluid to move in laminar flow along a fixed trajectory, resulting in limited gas / liquid two-phase contact area and low mixing efficiency. This is particularly problematic in scenarios requiring sufficient surface reaction, such as magnetic bead-peptide modification, which can easily lead to uneven modification and incomplete reaction, directly affecting the accuracy of detection data. Second, during the formation of water-in-oil droplets, traditional channels struggle to effectively control the dispersion of magnetic particles. Due to van der Waals forces and magnetic dipole interactions, magnetic beads are prone to uncontrolled aggregation, causing them to leak outside the oil film, severely impacting droplet uniformity and subsequent biomodification efficiency.

[0004] Especially in the magnetic bead-mediated peptide modification system, the existing technology faces a dual challenge: during the premixing stage, the magnetic beads generate local vortices due to the uneven distribution of shear force in the flow field, resulting in the formation of aggregates with a particle size >5μm; when the oil-water interface is formed, the traditional microdroplet generation structure is difficult to maintain the rotational flow dynamics of the magnetic beads, and the probability of the aggregated magnetic beads breaking through the surface tension of the oil film increases significantly, which seriously restricts the controllability of the peptide modification ratio. Utility Model Content

[0005] To address at least the above-mentioned technical problems existing in the prior art, this utility model provides a microfluidic chip device.

[0006] This utility model provides a microfluidic chip device, which consists of a substrate and a top plate. The substrate has an oil storage chamber, a magnetic bead storage chamber, a peptide storage chamber, a microdroplet storage chamber, a first mixing section and a second mixing section recessed thereon. The first mixing section has a first inlet and a first outlet. The magnetic bead storage chamber and the peptide storage chamber are connected to the first inlet through a flow channel. The oil storage chamber is connected to the first outlet through the convergence of two flow channels. The second mixing section has a second inlet and a second outlet. The second inlet is connected to the first outlet and the second outlet is connected to the microdroplet storage chamber.

[0007] In some embodiments, a plurality of interconnected cylindrical mixing chambers are provided between the first inlet and the first outlet of the first mixing section, and the mixing chambers are interconnected through a plurality of flow channels.

[0008] In some embodiments, the mixing chamber is provided with a first barrier column and a second barrier column, and a microchannel is formed between the first barrier column, the second barrier column and the inner wall of the mixing chamber.

[0009] In some embodiments, the top plate is provided with a first barrier column and a second barrier column that are not adjacent to each other. The first barrier column and the second barrier column are separately disposed in the mixing chamber, and a microchannel is formed between the first barrier column, the second barrier column and the inner wall of the mixing chamber.

[0010] In some embodiments, the first barrier post and the second barrier post have different diameters.

[0011] In some embodiments, the diameter of the second barrier post is smaller than the width of the flow channel.

[0012] In some embodiments, the top plate is provided with an oil inlet corresponding to the oil storage chamber, a magnetic bead inlet corresponding to the magnetic bead storage chamber, a polypeptide inlet corresponding to the polypeptide storage chamber, and a microdroplet outlet corresponding to the microdroplet storage chamber.

[0013] In some embodiments, the substrate and the top plate can be sealed together, and the substrate and the top plate are made of any one or more materials selected from polydimethylsiloxane (PDMS), quartz glass, polymethyl methacrylate (PMMA), or polycarbonate (PC).

[0014] In some embodiments, the channel width ranges from 20 μm to 80 μm, and the microchannel width ranges from 500 nm to 30 μm.

[0015] This invention provides a microfluidic chip device in which an oil storage chamber, a magnetic bead storage chamber, a peptide storage chamber, a microdroplet storage chamber, a first mixing section, a second mixing section, and a flow channel are fabricated on a substrate by etching or 3D printing. The substrate and top plate are then sealed and assembled. This microfluidic chip device simplifies the chip structure, reduces manufacturing costs and assembly complexity, and improves preparation efficiency.

[0016] In use, this microfluidic chip device involves an external liquid injection device injecting oil phase liquid into the oil inlet to enter the oil storage section, a magnetic bead solution into the magnetic bead inlet to enter the magnetic bead storage section, and a peptide solution into the peptide storage section. The magnetic bead solution and peptide solution are simultaneously injected into the first mixing section via hydraulic pressure from the external liquid injection device. The mixed magnetic beads and peptide solutions enter the mixing chamber through the first inlet channel. The microchannel formed by the first and second barrier columns creates a hindrance effect, changing the flow rate of the magnetic bead and peptide solution, causing the magnetic bead and peptide solution to rotate and mix before flowing out of the first outlet of the first mixing section. The oil phase liquid in the oil storage section merges with the magnetic bead and peptide solution through a dual channel at the first outlet of the first mixing section. Oil phase is injected into both sides of the dual channel, forming an oil phase encapsulating a water phase, resulting in an oil-encapsulated magnetic bead solution. The device utilizes a microfluidic chip design in the first mixing section to create a barrier column microchannel. Flow rate control enables rotational mixing of the magnetic bead peptide solution, significantly improving the uniformity of the mixing. The second mixing section extends the microdroplet flow through a second inlet into the second mixing section channel for the peptide modification reaction. This design extends the reaction time of the microdroplets by optimizing the flow channel, precisely controlling the peptide modification process and improving reaction efficiency. The entire process is hydraulically driven, avoiding complex manual operations and reducing the risk of cross-contamination. It also supports high-throughput sample processing. The microfluidic system significantly reduces reagent consumption and substantially lowers the cost per experiment.

[0017] It should be understood that the description herein is not intended to identify key or essential features of the embodiments of this disclosure, nor is it intended to limit the scope of this disclosure. Other features of this disclosure will become readily apparent from the following description. Attached Figure Description

[0018] The above and other objects, features, and advantages of the present invention will become readily apparent from the following detailed description of exemplary embodiments, taken in conjunction with the accompanying drawings. Several embodiments of the present invention are illustrated in the drawings by way of example and not limitation, in which:

[0019] In the attached figures, the same or corresponding labels indicate the same or corresponding indoor distribution.

[0020] Figure 1 This is a three-dimensional structural diagram of a microfluidic chip device according to an embodiment of the present invention. Figure 1 ;

[0021] Figure 2 This is a top view of the substrate of a microfluidic chip device according to an embodiment of the present invention;

[0022] Figure 3 This is a top view of the first mixing section of a microfluidic chip device according to an embodiment of the present invention;

[0023] Figure 4 This is a top view of the second mixing section of a microfluidic chip device according to an embodiment of the present invention;

[0024] Figure 5 This is a three-dimensional structural diagram of a microfluidic chip device according to an embodiment of the present invention. Figure 2 ;

[0025] In the picture:

[0026] 1: Substrate; 11: Oil storage chamber; 12: Magnetic bead storage chamber; 13: Peptide storage chamber; 14: Microdroplet storage chamber; 15: Second mixing section; 151: Second inlet; 152: Second outlet; 2: Top plate; 21: Oil inlet; 22: Magnetic bead inlet; 23: Peptide inlet; 24: Microdroplet outlet; 3: First mixing section; 31: Mixing chamber; 311: Microchannel; 32: Flow channel; 33: First barrier column; 34: Second barrier column; 35: First inlet; 36: First outlet. Detailed Implementation

[0027] To make the objectives, features, and advantages of this utility model more apparent and understandable, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are merely one-room embodiments of this utility model, not a full-room embodiment. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0028] like Figures 1 to 4 As shown, this utility model provides:

[0029] A substrate 1 and a top plate 2 are provided on the substrate 1. The substrate 1 has an oil storage chamber 11, a magnetic bead storage chamber 12, a peptide storage chamber 13, a microdroplet storage chamber 14, a first mixing section 3, and a second mixing section 15 recessed within the substrate 1. The first mixing section 3 has a first inlet 35 and a first outlet 36. The magnetic bead storage chamber 12 and the peptide storage chamber 13 are connected to the first inlet 35 via a flow channel. The oil storage chamber 11 is connected to the first outlet 36 via a confluence of two flow channels. The second mixing section 15 has a second inlet 151 and a second outlet 152. The second inlet 151 is connected to the first outlet 36, and the second outlet 152 is connected to the microdroplet storage chamber 14. The first mixing section 3 contains multiple mixing chambers 31, which are connected by flow channels 32. In the secondary connection, the mixing chamber 31 is provided with a columnar or other irregularly shaped first barrier column 33 and a second barrier column 34. The top ends of the first barrier column 33 and the second barrier column 34 are connected to the top plate 2, and the bottom ends of the first barrier column 33 and the second barrier column 34 are connected to the substrate 1. A microchannel 311 is formed between the first barrier column 33, the second barrier column 34 and the inner wall of the mixing chamber 31. The top plate 2 is provided with an oil inlet 21 corresponding to the oil storage chamber 11, a magnetic bead inlet 22 corresponding to the magnetic bead storage chamber 12, a peptide inlet 23 corresponding to the peptide storage chamber 13, and a microdroplet outlet 24 corresponding to the microdroplet storage chamber 14. The substrate 1 and the top plate 2 can be sealed and fastened together.

[0030] During assembly, the oil storage chamber 11, magnetic bead storage chamber 12, peptide storage chamber 13, microdroplet storage chamber 14, first mixing section 3, second mixing section 15 and flow channel are fabricated on the substrate 1 by etching or 3D printing, and the substrate 1 and top plate 2 are sealed and assembled.

[0031] In use, an external liquid injection device injects oil phase liquid into the oil inlet 21, which enters the oil storage section 11. An external liquid injection device also injects magnetic bead solution into the magnetic bead inlet 22, which enters the magnetic bead storage section 12. The magnetic beads are nano-sized, preferably in the range of 100-1000 nm. An external liquid injection device also injects peptide solution into the peptide inlet 23, which enters the peptide storage section 13. The peptides are peptide solutions with or without modified hydroxyl, amino, carboxyl, or other chemical bonds. The magnetic bead solution and peptide solution are simultaneously flowed along the flow channel to the first mixing section 3 by the hydraulic pressure of the external liquid injection device. The mixed magnetic beads and peptide solution enter the mixing chamber 31 through the first inlet 35 and the flow channel 32. The microchannel 311 formed by the first barrier column 33 and the second barrier column 34 creates a blocking effect, altering the magnetic bead density. The flow rate of the peptide solution causes the magnetic bead peptide solution to rotate and mix before flowing out to the first outlet 36 of the first mixing section 3. The oil phase liquid in the oil storage section 11 merges with the magnetic bead peptide solution through a dual-channel flow to the first outlet 36 of the first mixing section 3. The oil phase is injected into both sides of the dual-channel flow to form an oil phase encapsulating a water phase, generating microdroplets of oil-encapsulated magnetic bead peptide solution. The microdroplets enter the flow channel of the second mixing section 15 through the second inlet 151 to carry out the magnetic bead peptide modification reaction. At this time, external factors that interfere with the experiment can be applied to the second mixing section 15, including but not limited to changes in temperature, light, radiation, and vibration. The microdroplets flow along the flow channel of the second mixing section 15 to the second outlet 152 and enter the microdroplet storage section 14. The microdroplets are then drawn out by an external liquid suction device at the microdroplet outlet 24.

[0032] For example, such as Figure 2 As shown, the magnetic bead storage chamber 12 and the polypeptide storage chamber 13 are connected to the first inlet 35 of the first mixing section 3 through a T-shaped flow channel.

[0033] For example, the oil storage chamber 11, the magnetic bead storage chamber 12, the polypeptide storage chamber 13, and the microdroplet storage chamber 14 can be concave chamber structures in the shape of square columns, cylinders, or other columns.

[0034] For example, the mixing chamber 31 is a concave chamber structure with a circular or irregular shape.

[0035] For example, multiple mixing chambers 31 are etched on the substrate 1, each mixing chamber 31 is provided with a first barrier post 33 and a second barrier post 34, and the multiple mixing chambers 31 are connected in series through the flow channel 32.

[0036] For example, the diameters of the first barrier post 33 and the second barrier post 34 are different. Preferably, the diameter of the second barrier post 34 is smaller than the diameter of the first barrier post 33, and the diameter of the second barrier post 34 is smaller than the width of the flow channel 32.

[0037] For example, the first barrier column 33 has a larger diameter and is located in the middle of the mixing chamber 31, which creates a curved flow channel in the mixing chamber 31. The second barrier column 34 is located on the curved flow channel, forming a micro-channel 311, which produces a blocking effect and changes the flow velocity of the flow channel 32 and the micro-channel 311.

[0038] For example, the first barrier post 33 and the second barrier post 34 are fixedly etched on the substrate 1, and the tops of the first barrier post 33 and the second barrier post 34 are sealed to the top plate 2.

[0039] For example, at least two barrier columns may be installed in the mixing chamber 31.

[0040] For example, the oil storage chamber 11 is connected to the dual flow channel, the first outlet 36, and the second inlet 151.

[0041] For example, the dual channels connected to the oil storage chamber 11 need to be connected to both sides of the first outlet 36 of the first mixing section 3 in order to inject oil phase on both sides, form an oil phase encapsulating a water phase, and generate water-in-oil microdroplets.

[0042] For example, such as Figure 4 As shown, the second mixing section 15 is provided with a continuous flow channel containing straight segments and curved segments, which is used to prolong the magnetic bead-modified peptide reaction after the formation of microdroplets.

[0043] For example, the substrate 1 and the top plate 2 are made of any one or more materials selected from polydimethylsiloxane (PDMS), quartz glass, polymethyl methacrylate (PMMA), or polycarbonate (PC).

[0044] For example, the width of the flow channel 32 is in the range of 20μm-80μm, preferably 30μm, and the width of the microchannel 311 is in the range of 500nm-30μm, preferably 1μm.

[0045] For example, different functional reagents, such as antibodies and nucleic acids, can be injected into the polypeptide storage section 13.

[0046] In some embodiments of this utility model, the mixing chamber 31 of the first mixing part 3 is not provided with a barrier column integral with the substrate 1, and the top plate 2 is provided with a first barrier column 33 and a second barrier column 34 that are not adjacent to each other. The first barrier column 33 and the second barrier column 34 are separately arranged in the mixing chamber 31, and a microchannel 311 is formed between the first barrier column 33, the second barrier column 34 and the inner wall of the mixing chamber 31.

[0047] For example, such as Figure 5 As shown, the first barrier post 33 and the second barrier post 34 protrude from the same side surface of the top plate 2 and can be integrally formed or separately glued together. The height of the first barrier post 33 and the second barrier post 34 is the same as the recessed depth of the mixing chamber 31.

[0048] In the description of this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. Furthermore, the described specific features, structures, materials, or characteristics may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of those different embodiments or examples.

[0049] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this utility model, "a plurality of" means two or more, unless otherwise explicitly specified.

[0050] The above are merely specific embodiments of this utility model, but the protection scope of this utility model is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in this utility model should be included within the protection scope of this utility model. Therefore, the protection scope of this utility model should be determined by the scope of the claims.

Claims

1. A microfluidic chip device, comprising a substrate (1) and a top plate (2), characterized in that, The substrate (1) is recessed and embedded with an oil storage chamber (11), a magnetic bead storage chamber (12), a polypeptide storage chamber (13), a microdroplet storage chamber (14), a first mixing section (3), and a second mixing section (15). The first mixing section (3) is provided with a first inlet (35) and a first outlet (36). The magnetic bead storage chamber (12) and the polypeptide storage chamber (13) are connected to the first inlet (35) through a flow channel. The oil storage chamber (11) is connected to the first outlet (36) through the intersection of two flow channels. The second mixing section (15) is provided with a second inlet (151) and a second outlet (152). The second inlet (151) is connected to the first outlet (36), and the second outlet (152) is connected to the microdroplet storage chamber (14).

2. The microfluidic chip device according to claim 1, characterized in that, The first mixing section (3) has a plurality of interconnected cylindrical mixing chambers (31) between the first inlet (35) and the first outlet (36), and the mixing chambers (31) are connected through a plurality of flow channels (32).

3. The microfluidic chip device according to claim 2, characterized in that, The mixing chamber (31) is provided with a first barrier column (33) and a second barrier column (34), and a microchannel (311) is formed between the first barrier column (33), the second barrier column (34) and the inner wall of the mixing chamber (31).

4. The microfluidic chip device according to claim 2, characterized in that, The top plate (2) is provided with a first barrier column (33) and a second barrier column (34) that are not adjacent to each other. The first barrier column (33) and the second barrier column (34) are separately arranged in the mixing chamber (31). A microchannel (311) is formed between the first barrier column (33), the second barrier column (34) and the inner wall of the mixing chamber (31).

5. The microfluidic chip device according to claim 3 or 4, characterized in that, The first barrier post (33) and the second barrier post (34) have different diameters.

6. The microfluidic chip device according to claim 5, characterized in that, The diameter of the second barrier post (34) is smaller than the width of the flow channel (32).

7. The microfluidic chip device according to claim 1 or 4, characterized in that, The top plate (2) is provided with an oil inlet (21) corresponding to the oil storage chamber (11), a magnetic bead inlet (22) corresponding to the magnetic bead storage chamber (12), a polypeptide inlet (23) corresponding to the polypeptide storage chamber (13), and a microdroplet outlet (24) corresponding to the microdroplet storage chamber (14).

Images