Micro-fluidic chip device

By designing barrier column microchannels and hydraulic drive in a microfluidic chip device, the problems of uneven magnetic bead-peptide modification and incomplete reaction in the prior art have been solved, realizing efficient magnetic bead-peptide mixing and reaction control, improving detection accuracy and reducing costs.

CN224388822UActive Publication Date: 2026-06-23NANOMICS BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202521062458.X
Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
Filing Date
2025-05-28
Publication Date
2026-06-23
Estimated Expiration
2035-05-28

AI Technical Summary

Technical Problem

Existing microfluidic chips suffer from insufficient hydrodynamic control in multiphase mixing and microdroplet preparation, leading to uneven magnetic bead-peptide modification and incomplete reactions. In particular, magnetic beads tend to aggregate during the formation of water-in-oil microdroplets, affecting the accuracy of detection data and the efficiency of biomodification.

Method used

A microfluidic chip device was designed, comprising an oil storage chamber, a magnetic bead storage chamber, a peptide storage chamber, a microdroplet storage chamber, a first mixing section, and a second mixing section. Through the microchannels formed by the barrier column, the magnetic bead peptide solution is rotated and mixed by hydraulic pressure. The channel structure is optimized to prolong the reaction time and achieve efficient magnetic bead peptide modification.

Benefits of technology

It significantly improves the uniformity and reaction efficiency of magnetic bead and peptide mixing, reduces reagent consumption, simplifies the operation process, reduces the cost per experiment, and supports high-throughput sample processing.

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Abstract

The micro-fluidic chip device comprises a substrate and a top plate, an oil storage chamber, a magnetic bead storage chamber, a polypeptide storage chamber, a droplet storage chamber, a first mixing part and a second mixing part are arranged on the substrate in a concave embedding mode, the first mixing part is provided with a first inlet and a first outlet, and the second mixing part is provided with a second inlet and a second outlet. The first mixing part is provided with a first inlet and a first outlet, the magnetic bead storage chamber and the polypeptide storage chamber are communicated with the first inlet through a runner, the oil storage chamber is communicated with the first outlet through double-runner intersection, the second mixing part is provided with a second inlet and a second outlet, the second inlet is communicated with the first outlet, and the second outlet is communicated with the droplet storage chamber. According to the utility model, the chip structure is simplified, the manufacturing cost and the assembly complexity are reduced, the rotating mixing of the magnetic bead polypeptide solution is realized by utilizing the design of the blocking column micro-channel and regulating and controlling the flow speed, the mixing uniformity of the magnetic bead and the polypeptide is obviously improved, and meanwhile, the reagent consumption and the experiment cost are obviously reduced by the micro-fluidic system.
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