Fermenter with on-line bacteria separation

Abstract

The utility model discloses a kind of fermentation tanks with on-line working bacteria separation, including tank body, the tank body side is provided with material guiding pump, refrigerator and centrifuge, tank body bottom is equipped with outlet, and outlet is connected with the input end of first discharge pipe and material guiding pump respectively by three-way, the output end of material guiding pump is connected with refrigerator inlet by first pipeline, refrigerator outlet is connected with centrifuge inlet by second pipeline, centrifuge outlet is connected to tank body top by third pipeline, it is connected between first pipeline and third pipeline by fourth pipeline, second discharge pipe is also connected on third pipeline, it is connected between second pipeline and fourth pipeline by fifth pipeline, third discharge pipe is also connected on second pipeline;The fermentation tank is integrated by tank body, refrigerator and centrifuge Design, then cooperate the adjustment of various valves, can realize static microorganism separation, material liquid circulation, continuous on-line separation production etc. Function, make production process continuous, automation, improve production efficiency.

Description

Technical Field

[0001] This utility model relates to the field of fermentation tank technology, specifically to a fermentation tank capable of online separation of working bacteria. Background Technology

[0002] Microbial fermenters are key equipment for various microbial fermentation production activities. Currently, many microbial fermenters consist of separate fermentation tanks, refrigeration equipment, and separation equipment, lacking effective integration and connection. This decentralized equipment layout leads to significant discontinuities in the entire production process, making it impossible to achieve continuous and automated production. In addition, the existing separation equipment design of microbial fermenters cannot achieve the function of rotating and conveying materials simultaneously. That is, during centrifugation, it is impossible to simultaneously transport the liquid material. This design limits the application of centrifuges in continuous production, requiring frequent start-ups and shutdowns of centrifuges to load and unload materials, greatly reducing production efficiency.

[0003] To address these issues, we have designed a fermenter capable of online separation of working bacteria. Utility Model Content

[0004] The purpose of this invention is to address the shortcomings of existing technologies by providing a fermenter capable of online separation of working bacteria, thereby solving the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a fermenter capable of online separation of working bacteria, comprising a tank body, a feed pump, a refrigeration unit, and a centrifuge arranged beside the tank body, an outlet at the bottom of the tank body, and the outlet being connected to a first discharge pipe and the input end of the feed pump respectively via a tee, the output end of the feed pump being connected to the inlet of the refrigeration unit via a first pipe, the outlet of the refrigeration unit being connected to the inlet of the centrifuge via a second pipe, the outlet of the centrifuge being connected to the top of the tank body via a third pipe, the first pipe and the third pipe being connected via a fourth pipe, the third pipe being connected to a second discharge pipe, the second pipe and the fourth pipe being connected via a fifth pipe, and the second pipe being connected to a third discharge pipe.

[0006] As a preferred embodiment of this utility model, the centrifuge includes a shell, which is mounted on a tank via a fixing frame. A rotating drum is disposed inside the shell. A multi-faceted rotating tube, shaped like a hexagonal prism, is connected to the outlet at the top of the rotating drum. A discharge connecting pipe is rotatably connected to the top of the multi-faceted rotating tube, and the rotatable connection between the two is sealed by a top rotating shaft seal. The top rotating shaft seal is fixed to the discharge connecting pipe, which is connected to a third pipe via a flange clip. A belt-driven pulley and a pulley clamping component are fitted outside the multi-faceted rotating tube. The belt-driven pulley is located inside the pulley clamping component, which is fixed externally. On the shell, the top rotating shaft seal is fixed to the driven wheel clamping part. The bottom inlet of the drum is rotatably connected to the second pipe, and the rotatable connection between the two is sealed by the bottom rotating shaft seal. The bottom rotating shaft seal is fixed to the second pipe. A centrifugal motor is installed outside the shell, and a belt drive pulley is installed on its output shaft. The belt drive pulley and the belt driven pulley are connected by a transmission belt. A belt tension pulley is rotatably installed on the shell and is supported on the transmission belt. A belt pulley protective cover is also installed on the shell. The belt drive pulley, belt tension pulley, transmission belt and belt driven pulley are all located inside the belt pulley protective cover.

[0007] As a preferred technical solution of this utility model, the tank body is formed by connecting the tank body and the tank cover through a fastening flange. The top of the cover is provided with a sterile filter, an inoculation port, a feed port and an observation window. A Miller plate heat exchange layer is sleeved and fixed on the outside of the tank body. A sandwich layer is provided in the middle of the Miller plate heat exchange layer. A cooling water channel is provided in the sandwich layer. The cooling water outlet of the cooling water channel is located in the upper part of the Miller plate heat exchange layer, and the cooling water inlet of the cooling water channel is located in the lower part of the Miller plate heat exchange layer.

[0008] As a preferred embodiment of this utility model, a discharge valve is installed at the outlet of the tank.

[0009] As a preferred embodiment of this utility model, the first discharge pipe, the second discharge pipe, and the third discharge pipe are respectively equipped with a discharge port valve, a pre-centrifugation discharge valve, and a centrifuge outlet discharge valve.

[0010] As a preferred embodiment of this utility model, the second, third, fourth, and fifth pipes are respectively equipped with a centrifuge feed valve, a centrifuge liquid return valve, a liquid circulation pipeline valve, and a centrifuge pipeline valve. The centrifuge feed valve is located between the centrifuge and the second discharge pipe, the centrifuge liquid return valve is located between the fourth pipe and the third discharge pipe, and the liquid circulation pipeline valve is located between the third pipe and the fifth pipe.

[0011] As a preferred embodiment of this utility model, the inlet and outlet of the refrigeration unit are respectively equipped with cooling pipe valves and cold material outlet valves.

[0012] As a preferred technical solution of this utility model, the drum includes a drum body, a triangular guide plate and a bottom cover. The triangular guide plate is fixed inside the drum body, the bottom cover is fixed at the bottom of the drum body, the top outlet of the drum is located at the top of the drum body, and the bottom inlet of the drum is located at the bottom of the bottom cover.

[0013] As a preferred embodiment of this utility model, the passive wheel clamping component includes an upper clamping component and a lower clamping component. Both the upper and lower clamping components consist of an annular plate and four connecting posts. The radius of the central circular hole of the annular plate is larger than the radius of the circumscribed circle of the cross-section outside the polygonal rotating tube. The four connecting posts are fixed on the annular plate in a circular array, and each connecting post is connected to a threaded post at its end. The bottom of the circular hole of the annular plate of the upper clamping component and the top of the circular hole of the annular plate of the lower clamping component are provided with protrusions. The belt-driven passive wheel is sandwiched between two protrusions. The annular plate of the upper clamping component has four through holes. The threaded posts of the four connecting posts of the lower clamping component pass through these four through holes and are connected to nuts. The top rotating shaft seal also has four through holes. The threaded posts of the four connecting posts of the upper clamping component pass through these four through holes and are connected to nuts.

[0014] As a preferred technical solution of this utility model, the belt driven pulley is provided with an axial hexagonal prism hole, and the polygonal rotating tube can pass through the hexagonal prism hole, and its outer wall can be in close contact with the inner wall of the hexagonal prism through hole.

[0015] Compared with the prior art, this utility model provides a fermenter for online separation of working bacteria, which has the following beneficial effects:

[0016] 1. This fermenter, which can isolate microorganisms online, integrates the tank body, refrigeration unit, and centrifuge. With the adjustment of various valves, it can realize functions such as static microbial separation, liquid circulation, and continuous online separation production, making the production process continuous and automated, shortening the production cycle, and improving production efficiency.

[0017] 2. This fermenter, which can separate working bacteria online, uses a belt-driven passive pulley to drive a polygonal hollow rotating tube. With the addition of rotating shaft seals at both ends, the drum and the pipe can rotate and convey materials simultaneously, realizing the function of online real-time pipe separation. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0019] Figure 2 This is a schematic diagram of the top structure of the tank body of this utility model;

[0020] Figure 3 This is a schematic diagram of the centrifuge structure of this utility model;

[0021] Figure 4 This is a schematic diagram of the passive wheel clamping component of this utility model;

[0022] Figure 5 This is a schematic diagram of the drum structure of this utility model;

[0023] Figure 6 This is a schematic diagram showing the position of the triangular guide plate of this utility model.

[0024] Figure reference numerals: 1. Sterile filter; 2. Inoculation port; 3. Feed inlet; 4. Sight glass; 5. Cooling water outlet; 6. Miller plate heat exchange layer; 7. Discharge valve; 8. Discharge outlet valve; 9. Feed pump; 10. Feed circulation pipeline valve; 11. Centrifuge pipeline valve; 12. Cooling pipeline valve; 13. Refrigeration unit; 14. Cold material outlet valve; 15. Pre-centrifuge discharge valve; 16. Centrifuge feed valve; 17. Cooling water inlet; 18. Bottom rotary shaft seal; 19. Drum; 20. Centrifuge motor; 21. Belt drive pulley; 22. Belt tensioner; 23. Drive belt; 24. Belt pulley protective cover; 25. 26. Outer shell; 27. Flange clip; 28. Discharge connection pipe; 29. ​​Centrifuge discharge port valve; 30. Centrifuge liquid return valve; 31. Fastening flange; 32. Top rotating shaft seal; 33. Upper clamping part of driven wheel; 34. Belt driven wheel; 35. Lower clamping part of driven wheel; 36. Multi-angle rotating pipe; 37. Drum body; 38. Triangular guide plate; 39. Bottom cover; 40. Fixing frame; 41. Tank body; 42. Centrifuge; 43. First discharge pipe; 44. First pipeline; 45. Third pipeline; 46. Fourth pipeline; 47. Fifth pipeline; 48. Second discharge pipe; 49. Third discharge pipe. Detailed Implementation

[0025] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0026] Please see Figures 1-6In this embodiment: a fermenter capable of online separation of working microorganisms, the tank body 40 is formed by connecting the tank body and the tank cover via a fastening flange 30. The top of the tank cover is equipped with a sterile filter 1, an inoculation port 2, a feed inlet 3, and an observation mirror 4. The sterile filter 1 is used to ventilate the fermenter while ensuring that external microorganisms cannot enter the tank and cause microbial contamination during the air intake and exhaust process. The inoculation port 2 is used for inoculating engineered microorganisms, the feed inlet 3 is used for adding liquid, and the observation mirror 4 is used to observe the liquid level and fermentation dynamics inside the tank. A Miller plate heat exchange layer 6 is fitted and fixed to the outside of the tank body. It is welded from Miller plates, and the covering area of ​​the Miller plate heat exchange layer 6 is greater than 80% of the outer surface area of ​​the tank body. A sandwich layer is provided in the middle of the Miller plate heat exchange layer 6, and a cooling water channel is provided within the sandwich layer. The cooling water outlet 5 of the cooling water channel is located at the upper part of the Miller plate heat exchange layer 6. The inlet 17 is located at the lower part of the Miller plate heat exchange layer 6. The cooling water outlet 5 and the cooling water inlet 17 can be connected to an external circulating water device. In addition, an online temperature control device can be installed on the tank 41 to maintain the temperature of the fermenter. A feed pump 9, a chiller 13 and a centrifuge 41 are set next to the tank 40. The feed pump 9 is used to control the backflow of the feed liquid. The chiller 13 is a pipeline chiller. Its function is to freeze the engineered bacteria in the fermentation liquid before centrifuging, reduce the metabolism of the engineered bacteria, reduce the reproduction of the engineered bacteria, facilitate the centrifugation to separate the engineered bacteria, and better achieve the separation of bacteria and liquid. The centrifuge 41 is used to centrifuge and separate the engineered bacteria. The bottom of the tank 40 is provided with an outlet. A discharge valve 7 is installed at the outlet. The outlet is connected to the first discharge pipe 42 and the input end of the feed pump 9 through a tee. A discharge valve 8 is installed on the first discharge pipe 42. The output end of the feed pump 9 is connected to the inlet of the refrigeration unit 13 through the first pipe 43. The inlet and outlet of the refrigeration unit 13 are respectively equipped with a cooling pipe valve 12 and a cold material outlet valve 14. The outlet of the refrigeration unit 13 is connected to the inlet of the centrifuge 41 through the second pipe 44. The centrifuge feed valve 16 and the third discharge pipe 48 are installed on the second pipe 44. The centrifuge pre-discharge valve 15 is installed on the third discharge pipe 48. The centrifuge 41 includes a shell 25, which is mounted on the tank 40 by a fixing bracket 39. A drum 19 is provided inside the shell 25. The drum 19 includes a drum body 36, a triangular guide plate 37, and a bottom cover 38. The triangular guide plate 37 is connected to the inlet of the centrifuge 41 through the first pipe 43. The centrifuge 41 includes a drum body 36, a triangular guide plate 37, and a bottom cover 38. The drum body 36 is fixed to the inside of the drum body 36 with screws. The bottom cover 38 is fixed to the bottom of the drum body 36 with screws. The top outlet of the drum 19 is located at the top of the drum body 36, and the bottom inlet of the drum 19 is located at the bottom of the bottom cover 38. The top outlet of the drum 19 is connected to a polygonal rotating tube 35 in the shape of a hexagonal prism. The top of the polygonal rotating tube 35 is rotatably connected to a discharge connecting pipe 27, and the rotatable connection between the two is sealed by a top rotating shaft seal 31. The top rotating shaft seal 31 is fixed to the discharge connecting pipe 27. The discharge connecting pipe 27 is connected to a third pipe 45 through a flange clip 26. A centrifugal liquid return valve 29 and a second discharge pipe 49 are installed on the third pipe 45.The centrifuge outlet discharge valve 28 is installed on the second discharge pipe 49. A belt-driven pulley 33 and a pulley clamping component are fitted around the outside of the polygonal rotating pipe 35. The pulley clamping component includes an upper clamping component 32 and a lower clamping component 34. Both the upper and lower clamping components consist of an annular plate and four connecting posts. The radius of the central hole in the annular plate is larger than the radius of the circumscribed circle of the outer section of the polygonal rotating pipe 35. The four connecting posts are fixed to the annular plate in a circular array, and each connecting post has a threaded post at its end. Protrusions are provided at the bottom of the annular hole in the upper clamping component 32 and the top of the annular hole in the lower clamping component 34. The belt-driven pulley 33... Sandwiched between two protrusions, the belt-driven pulley 33 has an axial hexagonal prism hole. The polygonal rotating tube 35 can pass through this hexagonal prism hole, and its outer wall can be in close contact with the inner wall of the hexagonal prism through hole. The annular plate of the upper clamping member 32 of the driven pulley has four through holes. The threaded posts of the four connecting posts of the lower clamping member 34 of the driven pulley pass through these four through holes and are connected to nuts. The top rotating shaft seal 31 also has four through holes. The threaded posts of the four connecting posts of the upper clamping member 32 of the driven pulley pass through these four through holes and are connected to nuts. The lower clamping member 34 of the driven pulley clamping member is fixed to the outer shell 25 by screws. The bottom inlet of the drum 19 is rotatably connected to the second pipe 44, and the rotation of the two is... The moving connection is sealed by a bottom rotating shaft seal 18, which is fixed to the second pipe 44. A centrifugal motor 20 is installed outside the outer casing 25, and a belt drive pulley 21 is installed on its output shaft. The belt drive pulley 21 and the belt driven pulley 33 are connected by a transmission belt 23. A belt tensioner 22 is rotatably installed on the outer casing 25, and the belt tensioner 22 is supported on the transmission belt 23. A pulley protection cover 24 is also installed on the outer casing 25. The belt drive pulley 21, belt tensioner 22, transmission belt 23, and belt driven pulley 33 are all located inside the pulley protection cover 24. When the centrifugal motor 20 starts, the centrifugal motor 20 can drive the polygonal rotating tube 35 to rotate through the transmission belt 23, thereby driving the rotation of the centrifugal motor 20. As drum 19 rotates, the liquid enters from the bottom of drum 19. The triangular guide plate 37 inside drum 19 evenly distributes the liquid throughout. Under the centrifugal force of the rotating drum 19, microorganisms and other solid substances are thrown towards the edge of the drum, while the liquid flows to the polygonal rotating tube 35 and exits the centrifuge 41 through the discharge connection pipe 27. The first pipe 43 and the third pipe 45 are connected by a fourth pipe 46, on which a liquid circulation valve 10 is installed. The second pipe 44 and the fourth pipe 46 are connected by a fifth pipe 47, on which a centrifugal valve 11 is installed. By adjusting these valves, liquids for different purposes and functions can be transported.

[0027] Example 1: When it is necessary to circulate the liquid inside the tank, the outlet valve 7 and the liquid circulation pipeline valve 10 need to be opened, the discharge port valve 8, the centrifugal pipeline valve 11, the cooling pipeline valve 12 and the centrifugal liquid return valve 29 need to be closed, and the feed pump 9 needs to be started to transport the liquid at the bottom of the tank 40 to the upper part of the tank 40, so that the liquid inside the tank can circulate.

[0028] Example 2: When cooling is not required and centrifugation separation and reflux are needed to separate the bacteria, the discharge port valve 7, centrifuge pipeline valve 11, centrifuge feed valve 16, and centrifuge liquid reflux valve 29 can be opened, while the discharge port valve 8, liquid circulation pipeline valve 10, cooling pipeline valve 12, cold material outlet valve 14, pre-centrifugation discharge valve 15, and centrifuge discharge port discharge valve 28 can be closed. The feed pump 9 and centrifuge motor 20 can be started to achieve the function of centrifugation and reflux simultaneously, thus separating the engineered bacteria. At the same time, the total colony value can be controlled according to the fermentation situation in the tank. If reflux is not required, and the clarified liquid after centrifugation is directly discharged or the fermentation liquid product with a low total colony count is collected, the centrifuge liquid reflux valve 29 can be closed and the centrifuge discharge port discharge valve 28 can be opened, and the clarified fermentation liquid can be directly collected from the third discharge pipe 49.

[0029] Example 3: When fermentation is vigorous, the fermentation liquid needs to be frozen to reduce the metabolic rate of the engineered bacteria before centrifugation. In this case, the discharge port valve 7, cooling pipeline valve 12, cold material outlet valve 14, centrifuge feed valve 16, and centrifuge liquid reflux valve 29 need to be opened; the discharge port valve 8, liquid circulation pipeline valve 10, centrifuge pipeline valve 11, pre-centrifuge discharge valve 15, and centrifuge discharge port discharge valve 28 need to be closed. The refrigeration unit 13, centrifuge motor 20, and feed pump 9 need to be started to achieve the function of freezing, centrifuging, and reflux simultaneously, separating the engineered bacteria that have become dormant after freezing. If reflux is not required, the centrifuge liquid reflux valve 29 needs to be closed, and the centrifuge discharge port discharge valve 28 needs to be opened. The clarified fermentation liquid can then be collected directly from the third discharge pipe 49.

[0030] Example 4: When the material liquid is delivered and the material liquid in the pipeline needs to be drained, the discharge port valve 8 should be opened to discharge the material liquid in the pipeline. At the same time, if the fermentation tank needs to be rinsed, or if the material liquid or waste liquid in the fermentation tank needs to be discharged, it can also be discharged from the first discharge pipe 42 in this way. When the centrifuge 41 is stopped and the material liquid in the drum 19 and pipeline needs to be discharged, the pre-centrifuge discharge valve 15 should be opened to discharge the remaining material liquid in the drum 19.

[0031] Example 5: When disassembling the drum 19, turn off the power, stop the centrifugal motor 20, open the pre-discharge valve 15 to drain all the liquid in the drum 19 and pipes, remove the pulley protective cover 24, open the flange clip 26, remove the third pipe 45, remove the nut on the driven wheel clamping part, remove the top rotating shaft seal 31, and then separate the polygonal rotating tube 35 from the drum 19. Hold the drum 19 vertically and slowly lift it vertically upwards. When the lower part of the drum 19 separates from the bottom rotating shaft seal 18, the drum 19 can be taken out. Unscrew the bottom cover 38, remove and take out the triangular guide plate 37, and then rinse or scrape out the centrifuged solids separated in the drum body 36. When installing the drum 19, the process is exactly the opposite.

[0032] Example 6: In practical applications, this fermenter can achieve continuous feeding fermentation and continuous pipeline centrifugal separation production without stopping the machine. After the fermentation liquid sample reaches the maturity index, the liquid can be discharged in a segmented manner. The operation is the same as the room temperature separation of microorganisms after fermentation, but only half of the liquid is discharged for microbial separation. Then, the microbial enrichment or the separated liquid is collected, and then fresh liquid is pumped in through the feed inlet 3 to continue fermenting the mixed liquid in the tank. After the fermentation index reaches the standard, the product can be separated and produced again. This can be repeated continuously multiple times. At the same time, if online production is carried out, the product is produced by centrifugal discharge while feeding and discharge are carried out. The feeding speed and discharge speed are the same to ensure that the liquid level index in the tank 40 is consistent. The system controls the index in the tank 40 and the speed of the external centrifugal motor 20 and the speed of the feed pump 9 to achieve system control and ensure the continuous production of microorganisms or liquid.

[0033] Finally, it should be noted that the above description is merely a preferred embodiment of this utility model and is not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A fermenter for online separation of working bacteria, comprising a tank body (40), characterized in that: A feed pump (9), a refrigeration unit (13), and a centrifuge (41) are arranged next to the tank (40). The bottom of the tank (40) has an outlet, which is connected to the first discharge pipe (42) and the input end of the feed pump (9) respectively through a tee. The output end of the feed pump (9) is connected to the inlet of the refrigeration unit (13) through the first pipe (43). The outlet of the refrigeration unit (13) is connected to the inlet of the centrifuge (41) through the second pipe (44). The outlet of the centrifuge (41) is connected to the top of the tank (40) through the third pipe (45). The first pipe (43) and the third pipe (45) are connected through the fourth pipe (46). The third pipe (45) is also connected to the third discharge pipe (49). The second pipe (44) and the fourth pipe (46) are connected through the fifth pipe (47). The second pipe (44) is also connected to the second discharge pipe (48).

2. The fermenter for online working microbial isolation according to claim 1, characterized in that: The centrifuge (41) includes a shell (25), which is mounted on the tank (40) by a fixing bracket (39). A rotating drum (19) is provided inside the shell (25). The top outlet of the rotating drum (19) is connected to a polygonal rotating tube (35) in the shape of a hexagonal prism. The top of the polygonal rotating tube (35) is rotatably connected to a discharge connecting pipe (27), and the rotatable connection between the two is sealed by a top rotating shaft seal (31). The top rotating shaft seal (31) is fixed to the discharge connecting pipe (27). The discharge connecting pipe (27) is connected to a third pipe (45) by a flange buckle (26). A belt driven pulley (33) and a driven pulley clamping component are sleeved on the outside of the polygonal rotating tube (35). The belt driven pulley (33) is located inside the driven pulley clamping component, which is fixed on the shell (25). The top rotating shaft seal (31) is fixed to the top rotating shaft seal (35). The drum (19) is fixed on the passive wheel clamping part. The bottom inlet of the drum (19) is rotatably connected to the second pipe (44), and the rotatable connection between the two is sealed by the bottom rotating shaft seal (18). The bottom rotating shaft seal (18) is fixed to the second pipe (44). A centrifugal motor (20) is installed on the outside of the outer shell (25). A belt drive pulley (21) is installed on its output shaft. The belt drive pulley (21) and the belt passive pulley (33) are connected by a transmission belt (23). A belt tension pulley (22) is rotatably installed on the outer shell (25). The belt tension pulley (22) is supported on the transmission belt (23). A belt pulley protective cover (24) is also installed on the outer shell (25). The belt drive pulley (21), belt tension pulley (22), transmission belt (23) and belt passive pulley (33) are all located inside the belt pulley protective cover (24).

3. The fermenter for online working microbial isolation according to claim 1, characterized in that: The tank body (40) is formed by connecting the tank body and the tank cover through a fastening flange (30). The top of the cover is provided with a sterile filter (1), an inoculation port (2), a feed port (3) and an observation mirror (4). A Miller plate heat exchange layer (6) is fitted and fixed on the outside of the tank body. A sandwich layer is provided in the middle of the Miller plate heat exchange layer (6). A cooling water channel is provided in the sandwich layer. The cooling water outlet (5) of the cooling water channel is located on the upper part of the Miller plate heat exchange layer (6), and the cooling water inlet (17) of the cooling water channel is located on the lower part of the Miller plate heat exchange layer (6).

4. The fermenter for online separation of working bacteria according to claim 1, characterized in that: The outlet valve (7) is installed at the outlet of the tank (40).

5. A fermenter for online separation of working bacteria according to claim 1, characterized in that: The first discharge pipe (42), the second discharge pipe (48) and the third discharge pipe (49) are respectively equipped with a discharge port valve (8), a centrifuge pre-discharge valve (15) and a centrifuge outlet discharge valve (28).

6. A fermenter for online separation of working bacteria according to claim 1, characterized in that: Centrifuge feed valve (16), centrifuge liquid return valve (29), liquid circulation pipeline valve (10), and centrifuge pipeline valve (11) are respectively installed on the second pipeline (44), the third pipeline (45), the fourth pipeline (46), and the fifth pipeline (47). The centrifuge feed valve (16) is located between the centrifuge (41) and the second discharge pipe (48). The centrifuge liquid return valve (29) is located between the fourth pipeline (46) and the third discharge pipe (49). The liquid circulation pipeline valve (10) is located between the third pipeline (45) and the fifth pipeline (47).

7. A fermenter for online separation of working bacteria according to claim 1, characterized in that: The inlet and outlet of the refrigeration unit (13) are respectively equipped with a cooling pipe valve (12) and a cold material outlet valve (14).

8. A fermenter for online separation of working bacteria according to claim 2, characterized in that: The drum (19) includes a drum body (36), a triangular guide plate (37) and a bottom cover (38). The triangular guide plate (37) is fixed inside the drum body (36), and the bottom cover (38) is fixed at the bottom of the drum body (36). The top outlet of the drum (19) is located at the top of the drum body (36), and the bottom inlet of the drum (19) is located at the bottom of the bottom cover (38).

9. A fermenter for online separation of working bacteria according to claim 2, characterized in that: The passive wheel clamping component includes an upper passive wheel clamping component (32) and a lower passive wheel clamping component (34). Both the upper passive wheel clamping component (32) and the lower passive wheel clamping component (34) are composed of an annular plate and four connecting posts. The radius of the central circular hole of the annular plate is larger than the radius of the circumscribed circle of the cross-section outside the polygonal rotating tube (35). The four connecting posts are fixed on the annular plate in a circular array, and each connecting post is connected to a threaded post at its end. The bottom of the circular hole of the annular plate of the upper passive wheel clamping component (32) and The top of the annular plate of the lower clamping member (34) of the driven wheel is provided with a protrusion. The belt driven wheel (33) is sandwiched between two protrusions. The annular plate of the upper clamping member (32) of the driven wheel is provided with four through holes. The threaded posts of the four connecting posts of the lower clamping member (34) of the driven wheel pass through these four through holes and are connected with nuts. The top rotating shaft seal (31) is also provided with four through holes. The threaded posts of the four connecting posts of the upper clamping member (32) of the driven wheel pass through these four through holes and are connected with nuts.

10. A fermenter for online separation of working bacteria according to claim 2, characterized in that: The belt driven pulley (33) has an axial hexagonal prism hole, through which the polygonal rotating tube (35) can pass, and its outer wall can be in close contact with the inner wall of the hexagonal prism through hole.

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