Optical detection structure and portable fluorescent immunoassay analyzer

Abstract

The utility model discloses an optical detection structure and portable fluorescent immunoassay appearance. Optical detection structure includes first seat body, light source structure, second seat body, optical component, and the first seat body is formed with the first light guide hole and is linked with the first light guide hole first light hole, and second seat body is connected with first seat body, and is seted up with the second light guide hole opposite first light guide hole, and the light source structure's luminous part is close to first light hole setting, and optical component is located in first light guide hole and second light guide hole. The utility model integrates light source structure and optical component in one, compared with common 45 degree two -way spectroscope adds filter piece scheme, and the optical part required is less, and the structure is more compact, and the volume of whole optical detection structure is smaller, can be better applicable to portable fluorescent immunoassay appearance, and moreover, assembling is more convenient, can better guarantee the accuracy of the light hole and optical component alignment on light source structure.

Description

Technical Field

[0001] This utility model relates to the technical field of medical devices, specifically to an optical detection structure and a portable fluorescence immunoassay analyzer. Background Technology

[0002] Portable fluorescence immunoassay analyzers are instruments that utilize fluorescence technology to analyze biological samples. They are portable and can quickly obtain test results from the samples being tested. They can be used to detect pathogen antibodies, tumor markers, autoantibodies, etc., in biological samples such as serum and urine, aiding in the diagnosis of infectious diseases, tumors, and autoimmune diseases, such as detecting hepatitis B surface antigen and antibody. During testing, a portable fluorescence immunoassay analyzer uses excitation light (such as an LED lamp) to excite the analyte to produce fluorescence. When this light shines on the analyte, the fluorescent label is excited and emits fluorescence. The intensity of the fluorescence is detected by a detector to obtain the test result. An optical structure is placed between the analyte and the detector to process the light and ensure the reliability of the detection. Because portable fluorescence immunoassay analyzers are relatively small, while traditional optical structures are relatively complex, assembly is difficult when suitable for use in portable fluorescence immunoassay analyzers. Utility Model Content

[0003] To address the aforementioned technical problems, the main objective of this invention is to provide an optical detection structure and a portable fluorescence immunoassay analyzer, aiming to solve the problem that traditional optical structures are not suitable for assembling small portable fluorescence immunoassay analyzers.

[0004] To achieve the above objectives, this utility model proposes an optical detection structure, comprising:

[0005] The first base has a first light guide hole formed on it, and a first light passage hole connected to the first light guide hole is provided.

[0006] The second base is connected to the first base. The second base has a second light guide hole opposite to the first light guide hole and a second light passage hole connected to the second light guide hole.

[0007] The light source structure has a light-emitting part disposed near the first light-passing hole;

[0008] Optical components are disposed within the first light guide hole and the second light guide hole.

[0009] Optionally, the first light guide hole, the second light guide hole, the first light passage hole, the second light passage hole, and the optical component are all coaxially arranged.

[0010] Optionally, the light-passing aperture is elongated.

[0011] Optionally, the light source structure includes a light source control circuit board and two LEDs disposed on the first base. The light source control circuit board is disposed on one side of the second base, and the two LEDs are symmetrically disposed on both sides of the first light-passing hole, with the light-emitting surfaces of the two LEDs being inclined and opposite to each other.

[0012] Optionally, the optical component includes a first lens disposed in the first light guide hole, a second lens disposed in the second light guide hole, and a filter located between the first lens and the second lens. The first lens and the second lens are both semi-circularly arranged, each having a planar side and a convex side, and the convex side of the first lens and the convex side of the second lens are arranged opposite to each other.

[0013] Optionally, the first light guide hole is partially reduced at one end near the first light through hole to form a first step portion, and the planar side of the first lens is bonded to the first step portion; the second light guide hole is partially reduced at one end near the second light through hole to form a second step portion, and the planar side of the second lens is bonded to the second step portion.

[0014] Optionally, the first light guide hole is partially expanded at one end near the second light guide hole to form a third step portion, and the side of the filter facing the first lens abuts against the third step portion.

[0015] Optionally, a buffer is pressed onto the side of the filter facing the second lens, and the second seat is pressed onto the buffer.

[0016] Optionally, the buffer is arranged in a ring shape, and the inner diameter of the buffer is larger than the outer diameter of the first lens and smaller than the outer diameter of the filter.

[0017] This utility model also provides a portable fluorescence immunoassay analyzer, comprising:

[0018] case;

[0019] The aforementioned optical detection structure is movably disposed within the housing; and,

[0020] A driving structure is disposed within the housing and is drivingly connected to the first and / or second base of the optical detection structure, for driving the optical detection structure to move laterally along the housing.

[0021] The technical solution provided by this utility model has the following beneficial effects:

[0022] The optical detection structure provided by this utility model includes a first base, a second base, a light source structure, and optical components. The first base has a first light guide hole and a first light passage hole communicating with the first light guide hole. The second base has a second light guide hole. LEDs from the light source structure can be placed on both sides of the first light passage hole. A light source control circuit board is located on the second base. The optical components are respectively placed within the first and second light guide holes. The LEDs of the light source structure emit excitation light to illuminate the sample. The fluorescence emitted by the excited fluorescent marker passes through the first light passage hole and enters the optical components. After processing by the optical components, the light is transmitted to the detector through the second light passage hole for detection and evaluation. During assembly, the light source structure can be fixed to the first base first, and some of the optical components can be assembled into the second base simultaneously. Then, the first and second bases are connected and fixed, improving assembly efficiency. The described optical detection structure integrates the light source structure and optical components into one unit. Compared to the common 45° dihedral beam splitter plus filter solution, it requires fewer optical components, has a more compact structure, and the overall optical detection structure is smaller and simpler, making it better suited for portable fluorescence immunoassay analyzers. Furthermore, it is easier to assemble and better ensures the accuracy of alignment between the light-passing aperture on the light source structure and the optical components. Attached Figure Description

[0023] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on the structures shown in these drawings without creative effort.

[0024] Figure 1 A schematic diagram of an embodiment of an optical detection structure provided by this utility model;

[0025] Figure 2 for Figure 1 A schematic diagram of the optical detection structure described in the diagram from another perspective;

[0026] Figure 3 for Figure 1 A cross-sectional structural diagram of the optical detection structure described in the figure.

[0027] Figure 4 A schematic diagram of an optical detection structure disposed on the driving structure according to the present invention;

[0028] Figure 5This is a schematic diagram of a structure of an embodiment of a portable fluorescence immunoassay analyzer provided by this utility model;

[0029] Figure 6 for Figure 5 A schematic diagram of the exploded structure of the portable fluorescence immunoassay analyzer described herein.

[0030] Explanation of icon numbers:

[0031] 1000-Portable fluorescence immunoassay analyzer; 100-Optical detection structure; 1-First base; 11-First light guide hole; 12-First light passage hole; 13-First step; 14-Third step; 2-Light source structure; 21-Light source control circuit board; 22-LED lamp; 3-Second base; 31-Second light guide hole; 32-Second light passage hole; 33-Second step; 4-Optical components; 41-First lens; 42-Second lens; 43-Filter; 5-Buffer; 6-Drive structure; 61-Driver; 62-Transmission screw; 63-Guide shaft; 64-Transmission nut; 65-Guide sleeve; 66-Connecting block; 200-Housing.

[0032] The realization of the purpose, functional characteristics and excellent effects of this utility model will be further explained below in conjunction with specific embodiments and accompanying drawings. Detailed Implementation

[0033] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0034] It should be noted that if the embodiments of this utility model involve directional indication, the directional indication is only used to explain the relative positional relationship and movement of each component in a specific posture. If the specific posture changes, the directional indication will also change accordingly.

[0035] Furthermore, if the embodiments of this utility model involve descriptions such as "first" or "second," these descriptions are for descriptive purposes only and should not be construed as indicating or implying their relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined with "first" or "second" may explicitly or implicitly include at least one of those features. Additionally, the meaning of "and / or" throughout the text includes three parallel solutions; for example, "A and / or B" includes solution A, solution B, or a solution where both A and B are satisfied simultaneously. Furthermore, the technical solutions of the various embodiments can be combined with each other, but this must be based on the ability of those skilled in the art to implement them. When the combination of technical solutions is contradictory or impossible to implement, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection claimed by this utility model.

[0036] This invention provides an optical detection structure 100, which is suitable for a portable fluorescence immunoassay analyzer 1000. For details, please refer to... Figure 1 and Figure 2 In this embodiment, the optical detection structure 100 includes a first base 1, a light source structure 2, a second base 3, and an optical component 4. The first base 1 has a first light guide hole 11 and a first light passage hole 12 communicating with the first light guide hole. The second base 3 is connected to the first base 1 and has a second light guide hole 31 opposite to the first light guide hole 11 and a second light passage hole 32 communicating with the second light guide hole 31. The light-emitting part of the light source structure 2 is disposed near the first light passage hole 12. The optical component 4 is disposed in the first light guide hole 11 and / or the second light guide hole 31.

[0037] In this embodiment, a first light guide hole 11 is provided on the first base 1, and a first light passage hole 12 communicating with the first light guide hole 11 is provided. A second light guide hole 31 is formed on the second base 3. The LED lamp 22 of the light source structure 2 is disposed on both sides of the first light passage hole 12. The light source control circuit board 21 of the light source structure 2 is disposed on the second base 3. The optical component 4 is fixed in the first light guide hole 11 and the second light guide hole 31. The LED lamp 22 can emit excitation light to irradiate the sample. The fluorescence emitted by the fluorescent marker after excitation can pass through the first light passage hole 12 to enter the optical component 4. After the light is processed by the optical component 4, it is transmitted to the detector for detection and evaluation through the second light passage hole 32. During assembly, the light source structure 2 can be fixed on the first base 1 first, and part of the optical component 4 can be assembled into the second base 3. Then the first base 1 and the second base 3 are connected and fixed to improve the assembly efficiency. The described light source detection structure integrates the light source structure and optical components into one unit. Compared to the common 45° dihedral beam splitter plus filter solution, it requires fewer parts, has a more compact structure, and the overall optical detection structure is smaller and simpler, making it better suited for portable fluorescence immunoassay analyzers. Furthermore, it is easier to assemble and better ensures the accuracy of alignment between the light-passing apertures on the light source structure and the optical components.

[0038] The shapes of the first seat 1 and the second seat 3 are matched, and both are approximately cuboid in shape. When the optical detection structure 100 is in normal use, the second seat 3 is positioned above the first seat 1 and is fitted against it. Unless otherwise specified, all descriptions of orientation in this invention shall be taken as such.

[0039] Preferably, such as Figure 3 As shown, the first light guide hole 11, the second light guide hole 31, the first light passage hole 12, the second light passage hole 32 and the optical component 4 are all coaxially arranged, which makes the optical path distance shorter and the fluorescence effect obtained better.

[0040] Furthermore, the first light-passing aperture 12 and the second light-passing aperture 32 are elongated to match the shape of the sample being tested, thereby allowing the fluorescence emitted from various parts of the sample after excitation to enter the optical component 4 more uniformly. Preferably, the first light-passing aperture 12 and the second light-passing aperture 32 are oblong apertures.

[0041] Specifically, the light source structure 2 is mainly used to emit excitation light onto the sample being tested. Combined with... Figure 2 and Figure 4As shown, the light source structure 2 includes a light source control circuit board 21 and two LEDs 22 mounted on the first base 1. The light source control circuit board 21 is located on one side of the second base 3. The light-emitting surfaces of the two LEDs are arranged at an angle to each other, and the two LEDs 22 are arranged symmetrically in a V-shape on both sides of the first light-passing aperture 12. This arrangement simplifies installation by directly fixing the light source control circuit board 21 to one side of the second base 3 and allows for a more compact installation with the LEDs 22. The light source control circuit board 21 can adjust the LED light intensity to adapt to the excitation light intensity required by different analytes. The V-shaped arrangement of the two LEDs 22 ensures that the excitation light can more evenly cover the sample, and the excited fluorescence can be transmitted more vertically through the first light-passing aperture 12 to the detector via the optical component 4, improving collection efficiency. Simultaneously, it avoids direct reflection of the excitation light to the detector, preventing background noise and improving the signal-to-noise ratio, resulting in more accurate and reliable detection results.

[0042] The optical component 4 is used to process the fluorescence emitted by the sample after excitation, so that the fluorescence signal can be transmitted more concentratedly and effectively to the detector of the portable fluorescence immunoassay analyzer 1000. Specifically, in conjunction with Figure 3 and Figure 4 As shown, the optical component 4 includes a first lens 41 disposed within a first light guide hole 11, a second lens 42 disposed within a second light guide hole 31, and a filter 43 disposed between the first lens 41 and the second lens 42. Both the first lens 41 and the second lens 42 are semi-circularly arranged, each having a planar side and a convex side. The convex side of the first lens 41 and the convex side of the second lens 42 are positioned opposite each other. When the sample is excited, the emitted fluorescence passes through the first light guide hole 12 and, through the first lens 41 and the second lens 42, can be focused and collected by the detector. Simultaneously, the filter 43, a bandpass filter, is disposed between the first lens 41 and the second lens 42, effectively filtering out some reflected excitation light and stray light, thus improving fluorescence collection efficiency.

[0043] Furthermore, the first light guide hole 11 is partially narrowed at the end near the first light passage hole 12 to form a first step portion 13, and the planar side of the first lens 41 abuts against the first step portion 13. The second light guide hole 31 is partially narrowed at the end near the second light passage hole 32 to form a second step portion 33, and the planar side of the second lens 42 is bonded to the second step portion 33, making the installation of the first lens 41 and the second lens 42 more stable and better secured within the first light guide hole 11 and the second light guide hole 31. Moreover, the first light guide hole 11 is partially expanded at the end near the second light guide hole 31 to form a third step portion 14, and the side of the filter 5 facing the first lens 41 abuts against the third step portion 14. This ensures the axial position of the first lens 41, the second lens 42, and the filter 5 in the entire fluorescence collection light path. The circumferential surface of the first lens 41 is fitted with the first light guide hole 11 with a small gap, and the circumferential surface of the second lens 42 is fitted with the second light guide hole 31 with a small gap. This ensures that the optical axes of the first lens 41 and the second lens 42 are aligned in the center, thus ensuring the coaxiality of the entire optical path.

[0044] Furthermore, during actual installation, the first lens 41 can be guided into the first base 1 through the first light guide hole 11 and bonded to the first step portion 13. The second lens 42 can be guided into the second base 2 through the second light guide hole 31 and bonded to the second step portion 33. Then, the filter 43 is placed on the third step portion 14. A buffer member 5 is pressed onto the side of the filter 43 facing the second lens 42, and the second base 3 is pressed onto the buffer member 5. Preferably, the buffer member 5 is arranged in a ring shape, and the inner diameter of the buffer member 43 is larger than the outer diameter of the first lens 41 and smaller than the outer diameter of the filter 43. When the first base 1 and the second base 3 are connected by screws or bolts, the second base 3 presses against the buffer member 5, thereby pressing the filter 43 to fix it and ensure the stability of the entire optical path. Installation is also convenient.

[0045] Furthermore, the buffer 5 is configured as an O-ring, which can evenly press against the filter 43, preventing damage to the filter 43. On the other hand, it ensures that the filter 43 is placed flat on the third step 14, preventing it from tilting and guaranteeing the coaxiality of the entire optical path. Simultaneously, the filter 43 is movably connected to the first base 1, not glued. When the filter 43 is damaged, or the object being detected is changed, and a different wavelength of fluorescence signal needs to be collected, the screws on the second base 3 can be quickly unscrewed to replace the filter.

[0046] Furthermore, only the filter 43 is used in the entire optical path, and the angle is 0°. There is no need to use the traditional 45° dihedral beam splitter and filter, which makes the installation more convenient and the cost lower. At the same time, the entire optical path distance is shortened, making the overall structure more compact and the instrument smaller.

[0047] This utility model also provides a portable fluorescence immunoassay analyzer 1000. Combined with... Figure 5 and Figure 6 As shown, the portable fluorescence immunoassay analyzer 1000 includes a housing 200, the aforementioned optical detection structure 100, and a driving structure 6. The optical detection structure 100 is movably disposed within the housing 200. The driving structure 6 is disposed within the housing 200 and is drivenly connected to the first base 1 and / or the second base 3 of the optical detection structure 100. It is used to drive the optical detection structure to move laterally along the housing 200, so that the position of the optical detection structure 100 can be flexibly adjusted, thereby better aligning it with the sample for easier detection.

[0048] Among them, such as Figure 4 As shown, the drive structure 6 includes a driver 61, a transmission screw 62, a guide shaft 63, a transmission nut 64, and a guide sleeve 65. The transmission nut 64 is screwed onto the transmission screw 62. The connecting block 66 connects the transmission nut 64 and the optical detection structure 100. The driver 61 drives the transmission screw 62 to rotate, causing the transmission nut 64 to move along the transmission screw 62. The connecting block 66 drives the optical detection structure 100 to move, so that the optical detection structure 100 can move along with the transmission nut 63.

[0049] Specifically, the drive structure 6 further includes a guide shaft 63 and a guide sleeve 65 sleeved on the outside of the guide shaft 63. The guide sleeve 65 is disposed on the transmission nut 64. The guide shaft 63 is parallel to the transmission lead screw 62. When the driver 61 drives the transmission nut 64, the guide sleeve 65 and the guide shaft 63 provide a guiding fit, making the movement of the optical detection structure 100, which is fixed to the transmission nut 64 by the connecting block 66, more stable and less prone to shaking.

[0050] Two guide sleeves 65 are slidably fitted onto two guide shafts 63 in a one-to-one correspondence. The two guide shafts 63 are symmetrically arranged on the transmission screw 62, which can share the load, reduce bending deformation of the transmission screw 62, and improve its service life. At the same time, the symmetrical arrangement of the two guide shafts 63 can better maintain the parallelism of the transmission nut 64, avoid tilting, and ensure the accuracy of the linear motion of the optical detection structure 100.

[0051] The above description is only a preferred embodiment of the present utility model and does not limit the patent scope of the present utility model. Any equivalent structure made using the contents of the present utility model specification and drawings, or directly or indirectly applied to other related technical fields, are similarly included within the patent protection scope of the present utility model.

Claims

1. An optical detection structure, characterized in that, include: The first base has a first light guide hole formed on it, and a first light passage hole connected to the first light guide hole is provided. The second base is connected to the first base. The second base has a second light guide hole opposite to the first light guide hole and a second light passage hole connected to the second light guide hole. The light source structure has a light-emitting part disposed near the first light-passing hole; Optical components are disposed within the first light guide hole and the second light guide hole.

2. The optical detection structure of claim 1, wherein, The first light guide hole, the second light guide hole, the first light passage hole, the second light passage hole, and the optical component are all arranged coaxially.

3. The optical detection structure of claim 1, wherein, The light-passing aperture is elongated and narrow.

4. The optical detection structure of claim 1, wherein, The light source structure includes a light source control circuit board and two LEDs disposed on the first base. The light source control circuit board is disposed on one side of the second base. The two LEDs are symmetrically disposed on both sides of the first light-passing hole, and the light-emitting surfaces of the two LEDs are inclined and opposite to each other.

5. The optical detection structure of claim 1, wherein, The optical components include a first lens disposed in the first light guide hole, a second lens disposed in the second light guide hole, and a filter located between the first lens and the second lens. The first lens and the second lens are both semi-circular and each has a planar side and a convex side. The convex side of the first lens and the convex side of the second lens are arranged opposite to each other.

6. The optical detection structure of claim 5, wherein, The first light guide hole is partially reduced at one end near the first light through hole to form a first step portion, and the planar side of the first lens is bonded to the first step portion; the second light guide hole is partially reduced at one end near the second light through hole to form a second step portion, and the planar side of the second lens is bonded to the second step portion.

7. The optical detection structure of claim 5, wherein, The first light guide hole is partially expanded at one end near the second light guide hole to form a third step portion, and the side of the filter facing the first lens abuts against the third step portion.

8. The optical detection structure of claim 5, wherein, A buffer is pressed onto the side of the filter facing the second lens, and the second seat is pressed onto the buffer.

9. The optical detection structure of claim 8, wherein, The buffer is arranged in a circular shape, and the inner diameter of the buffer is larger than the outer diameter of the first lens and smaller than the outer diameter of the filter.

10. A portable fluorescence immunoassay analyzer characterized by comprising: include: case; The optical detection structure as described in any one of claims 1 to 9 is movably disposed within the housing; as well as, A driving structure is disposed within the housing and is drivingly connected to the first and / or second base of the optical detection structure, for driving the optical detection structure to move laterally along the housing.

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