A novel production scale-up adsorption separation device

Abstract

A novel production scale-up adsorption separation device relates to the technical field of bioengineering product adsorption separation, and the technical scheme is: a filter is provided, the filter comprises an outer column pipe, an inner column pipe is arranged in the outer column pipe, and a hole is arranged in the inner column pipe; the filter is one or more than one; when the filter is one, a connecting piece is arranged in the middle of the outer column pipe, and N baffles are arranged on the connecting piece; the beneficial effects of the technical scheme are that the addition of the baffles enables the inner column pipe to form a relatively independent processing space, and liquid is circulated and communicated outside, so that the stability and uniformity of the fluid in the column pipe during the processing process are ensured, the production scale is expanded, the advantages of the original single equipment are guaranteed, the production land is saved, a plurality of single equipment is connected in series, each step is coordinated, the production efficiency is greatly improved, the utilization efficiency of the equilibrium liquid, the eluent and the like is improved, the cost is saved, and long-time automatic operation is realized.

Description

Technical Field

[0001] This utility model relates to the field of adsorption and separation technology for bioengineering products, and in particular to a novel adsorption and separation device for production scale-up. Background Technology

[0002] With the rapid development of bio-manufacturing, represented by modern bio-fermentation technology and synthetic biology, it has shown enormous application potential in fields such as medicine, energy, and chemicals. Furthermore, with continuous innovation in bio-manufacturing technology, the types of products and application areas are constantly expanding, and the market size is increasing year by year. Biopharmaceuticals involve genetic engineering, fermentation engineering, cell engineering, enzyme engineering, etc. With technological advancements and continuous expansion of production capacity, a large amount of raw materials will require immediate and efficient processing. In addition, extracting effective medicinal components from human blood, urine, tissue fluid, and other usable animal and plant blood, emulsions, and tissue extracts has always been an important aspect of modern bioengineering technology.

[0003] Application No. 2024105976146 discloses a fermentation broth post-treatment adsorption separation device and method. This method combines the specific chemical selective adsorption separation of adsorption packing with the sieving separation of filter screens, enabling the adsorption and desorption of effective components and the removal of a large number of impurities to be completed in one device. Although the device can achieve continuous operation, the cycle of packing balancing, target adsorption, impurity filtration and cleaning, target elution, and packing regeneration during the operation of a single unit prolongs the processing time per batch. Therefore, for production with a daily processing capacity of tons or more, the production efficiency is low, material waste is increased, and operating costs are increased. Utility Model Content

[0004] The purpose of this invention is to overcome the shortcomings of current filters, such as processing fewer products at a time, low efficiency, and increased material costs, and to propose a new type of adsorption separation device for large-scale production.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] A novel production scale-up adsorption separation device includes a filter. The filter includes an outer column tube, an inner column tube inside the outer column tube, and holes inside the inner column tube. The filter can be one or more. When there is only one filter, a connector is provided in the middle of the outer column tube. The connector is provided with N baffles, which divide the outer column tube into N+1 intervals. Each interval is provided with an inner column tube, where N is a natural number greater than or equal to 1.

[0007] When there is one or more filters, the filters are connected through an outlet pipe.

[0008] Multiple baffles are installed inside the outer column tube to form an interval zone, and an inner column tube is installed inside the interval zone. This way, when the capacity of the outer column tube is increased, it can still be processed quickly, thus improving processing efficiency.

[0009] Preferably, a motor is installed above the outer column tube, and the motor is installed correspondingly to the inner column tube, with the motor's output shaft connected to the inner column tube.

[0010] Preferably, the outer surface of the inner column tube is covered with a screen.

[0011] Preferably, when there is one or more filters, a connector is provided in the middle of the outer column tube, and N baffles are provided on the connector. The baffles divide the outer column tube into N+1 intervals, and an inner column tube is provided in each interval. N is a natural number greater than or equal to 1.

[0012] Connecting multiple filters involves calculating the required number of filters based on production scale and pre-experiment data. The selected filters are then connected end-to-end through outlet pipes. Each outlet pipe sequentially completes the above process segments. Simultaneously, each individual unit is sequentially operating in different process segments, allowing unused equilibrium liquid to be reused, improving solution utilization and reducing costs.

[0013] Preferably, the filter further includes a balance liquid pipe, a raw liquid pipe, an eluent pipe, and a regenerated liquid pipe. The raw liquid pipe, the eluent pipe, and the regenerated liquid pipe are respectively connected to the filter through a delivery pipe. The outlet of the delivery pipe is connected to an outlet pipe. A liquid pump, a first valve, and a second valve are sequentially connected to the outlet pipe. The outlet of the liquid pump is connected to the inlet of the filter, and the filter is connected to the outlet pipe.

[0014] Preferably, it also includes a collection pipe and a waste liquid pipe, with the first outlet of the liquid outlet pipe connected to the collection pipe and the second outlet of the liquid outlet pipe connected to the waste liquid pipe.

[0015] Preferably, a solenoid valve is installed at the connection between the collection pipe and the waste liquid pipe and the outlet pipe.

[0016] Preferably, the first outlet and the second outlet of the liquid outlet pipe are located between the first valve and the second valve.

[0017] Compared with the prior art, the beneficial effects of this utility model are as follows: the addition of baffles allows each inner column tube to form a relatively independent processing space, while also having external liquid circulation, ensuring the stability and uniformity of the fluid in the column tube during the processing. This expands the production scale while maintaining the advantages of the original single equipment, saving production land. The series connection of multiple single equipment and the coordination of each step greatly improves production efficiency, helps to improve the utilization efficiency of equilibrium liquid, eluent, etc., saves costs, and enables long-term automatic operation. Furthermore, while increasing the filter volume, multiple filters are also connected in series, further increasing the production scale and improving production efficiency. Attached Figure Description

[0018] To more clearly illustrate the technical solution of this utility model, the drawings used in the description will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0019] Figure 1 This is a schematic diagram of the structure inside the outer column tube in a specific embodiment of this utility model.

[0020] Figure 2 This is a schematic diagram of a specific embodiment of the present invention, showing that there are three baffles.

[0021] Figure 3 This is a schematic diagram of a structure in a specific embodiment of the present invention, showing that there are five baffles.

[0022] Figure 4 This is a schematic diagram of a structure in a specific embodiment of the present invention, which has six baffles.

[0023] Figure 5 This is a schematic diagram of the filter and pipeline conveying structure in a specific embodiment of this utility model.

[0024] Figure 6 This is a schematic diagram of the structure of multiple filter combinations in a specific embodiment of this utility model.

[0025] In the diagram: 1. Equilibrium liquid pipe; 2. Raw liquid pipe; 3. Elution liquid pipe; 4. Regenerated liquid pipe; 5. Collection pipe; 6. Waste liquid pipe; 7. Filter; 8. Discharge pipe; 9. Delivery pipe; 10. Feed pipe; 11. Liquid pump; 12. First valve; 13. Second valve; 14. Outer column pipe; 15. Inner column pipe; 16. Baffle; 17. Motor; 18. Connecting parts. Detailed Implementation

[0026] To make the objectives, features, and advantages of this utility model more apparent and understandable, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings of the specific embodiments. Obviously, the embodiments described below are only some embodiments of this utility model, and not all embodiments. Based on the embodiments of this patent, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this patent.

[0027] Example 1

[0028] Reference Figures 1-4A novel production scale-up adsorption separation device includes a filter 7, which comprises an outer column tube 14 and an inner column tube 15 inside the outer column tube 14. The inner column tube 15 has holes. When there is only one filter 7, a connector 18 is provided in the middle of the outer column tube 14. N baffles 16 are provided on the connector 18, dividing the outer column tube 14 into N+1 intervals. Each interval contains an inner column tube 15, where N is a natural number greater than or equal to 1. The number of baffles 16 is determined by the outer column tube 14. Each baffle 16 has a certain amount of clearance from the side wall, top, and bottom of the outer column tube 14. The addition of the baffles 16... The column tube 14 forms multiple relatively independent processing spaces, which are interconnected by external liquid circulation, ensuring the stability and uniformity of the fluid inside the column tube during the processing. The top of the filter 7 is provided with an inlet, and the bottom of the filter 7 is provided with multiple outlets, which are set in accordance with the intervals. Each of the multiple outlets is provided with an outlet pipe. The filter 7 is provided with only one inlet. Since the intervals are interconnected, it is convenient to fill the entire filter 7 with solution. At the same time, multiple outlets are provided, and the outlets are connected to the liquid outlet pipe 8 through multiple outlet pipes. The solution can be quickly discharged through the bottom to avoid accumulation in a certain position. The inlet is connected to the inlet pipe 10.

[0029] A motor 17 is installed above the outer column tube 14, and the motor 17 is correspondingly installed with the inner column tube 15. The output shaft of the motor 17 is connected to the inner column tube 15. The inner column tube 15 has multiple holes evenly distributed on its side, and the outer surface of the inner column tube 15 is covered with a screen. The screen size is selected according to the size of the particles to be separated. The upper section of the inner column tube 15 is connected to the motor 17 through a solid shaft dynamic seal through the upper flange, and the lower section is connected to the lower pipeline through a hollow tube dynamic seal through the lower flange. The increase in the diameter of the outer column tube 14 and the increase in the number of inner column tubes 15 ensure the expansion of production capacity, which not only expands the production scale but also maintains the advantages of the original single equipment.

[0030] Example 2

[0031] Reference Figures 5-6Unlike Embodiment 1, this embodiment includes multiple filters 7. Each filter 7 is connected via an outlet pipe 8, and adjacent outlet pipes 8 are interconnected. The embodiment also includes multiple filters 7, a balance liquid pipe 1, a raw liquid pipe 2, an eluent pipe 3, and a regenerated liquid pipe 4. The raw liquid pipe 2, eluent pipe 3, and regenerated liquid pipe 4 are connected to the filters 7 via multiple delivery pipes 9. The balance liquid pipe 1 delivers the balance liquid, the raw liquid pipe 2 delivers the feed liquid, the eluent pipe 3 delivers the eluent, and the regenerated liquid pipe 4 delivers the regenerated liquid. The outlet of each delivery pipe 9 is connected to an outlet pipe 8. A liquid pump 11, a first valve 12, and a second valve 13 are sequentially connected to the outlet pipe 8. The outlet of the liquid pump 11 is connected to the inlet of the filters 7 via a feed pipe 10. The outlet of the filters 7 is connected to the outlet pipe 8, located between the first valve 12 and the second valve 13. Adjacent outlet pipes 8 are connected via the second valve 13. During operation, the solution needs to be delivered to the filters. When the solution is in filter 7, close the first valve 12 and the second valve 13 on the corresponding outlet pipe 8. The solution is then transported to filter 7 through the delivery pipe 9 and the corresponding liquid pump 11 for reaction. When the solution in filter 7 needs to be transported to the next filter 7, close the first valve 12 on the corresponding outlet pipe 8, open the second valve 13, and simultaneously close the first valve 12 on the next outlet pipe 8. The solution is then transported to the corresponding filter 7 through the outlet pipe 8. When waste liquid needs to be discharged, close the first valve 12 and the second valve 13, close the corresponding valve on the collection pipe 5, and open the valve on the waste liquid pipe 6 to discharge the waste liquid. When the collected solution needs to be transported to the collection pipe 5, continue to close the first valve 12 and the second valve 13, open the corresponding valve on the collection pipe 5, close the valve on the waste liquid pipe 6, and transport the collected crude product to the collection pipe 5. The collected product is then processed again.

[0032] It also includes a collection pipe 5 and a waste liquid pipe 6, each equipped with a corresponding solenoid valve. When the final product or waste liquid needs to be discharged, the first valve 12 and the second valve 13 are closed, and the corresponding solenoid valves on the collection pipe 5 and the waste liquid pipe 6 are opened to discharge the solution. The first outlet of multiple outlet pipes 8 is connected to the collection pipe 5, and the second outlet of multiple outlet pipes 8 is connected to the waste liquid pipe 6. Before combining multiple filters 7, detailed parameters such as the amount of each liquid used, column transit time, pressure, and product concentration are pre-tested when the equilibrium cleaning solution, stock solution, eluent, and regenerated solution pass through one filter 7. Based on the measured parameters, it was determined that four filters 7 would be used in combination, and then connected end to end through pipelines and valves to form a closed loop. Multiple filters 7 were connected in series to form a cascade adsorption separation device. By connecting multiple small filters 7 in series and coordinating each step, production efficiency was greatly improved. This helped to improve the utilization efficiency of equilibrium liquid and eluent, save costs, and achieve long-term automatic operation. Through programming control of pipeline and valve switching, the device could automatically complete the sequential cycle of several process stages: packing balancing, target adsorption, impurity filtration and cleaning, target elution, and packing regeneration.

[0033] Taking the separation of urokinase as an example, the adsorption and separation packing material uses creatine as the affinity ligand. The equilibrium liquid tube 1 delivers the equilibrium liquid to the first filter 7 through the delivery tube 9 to balance the packing material in the first filter 7. After balancing, the first valve 12 is closed, and the second valve 13 and the liquid pump 11 on the second filter 7 are opened to deliver the remaining equilibrium liquid to the second filter 7. At the same time, the original solution is delivered to the first filter 7, and stirring begins in the first filter 7 to combine the packing material with the original solution, thereby adsorbing and separating the material.

[0034] After the reaction in the first filter 7, the raw liquid is transported to the waste liquid pipe 6 through the outlet pipe 8. At the same time, the equilibrium liquid is transported back to the first filter 7 through the equilibrium liquid pipe 1. The equilibrium liquid removes impurities from the packing material on the first filter 7. After the equilibrium liquid in the second filter 7 is used up, the equilibrium liquid is transported to the third filter 7. The third filter 7 starts to work. At the same time, the second filter 7 starts to transport raw liquid through the raw liquid pipe 2 to adsorb and separate materials.

[0035] After the stock solution in the second filter 7 is completely adsorbed, it is transported to the waste liquid pipe 6 through the outlet pipe 8. At the same time, the equilibrium liquid in the first filter 7 is transported back to the second filter 7. Also, according to the usage requirements, a portion of the equilibrium liquid is transported to the second filter 7 through the equilibrium liquid pipe 1 to remove impurities from the packing material in the second filter 7. Meanwhile, the first filter 7 begins to transport the eluent through the eluent pipe 3. The eluent adsorbs the material on the adsorbed packing material. After adsorption, it is transported to the collection pipe 5 through the outlet pipe 8 for collection. The third filter 7 transports the equilibrium liquid to the fourth filter 7, and at the same time, the stock solution is transported to the third filter 7 through the stock solution pipe 2.

[0036] The first filter 7 is supplied with regenerant through regenerant pipe 4 to regenerate the particles on the first filter 7; the second filter 7 is supplied with eluent through eluent pipe 3 to adsorb the material on the adsorbent packing. After adsorption, the eluent is supplied to the collection pipe 5 through outlet pipe 8. Part of the equilibrium liquid is supplied to the third filter 7 through equilibrium liquid pipe 1 to balance the third filter 7. At the same time, the fourth filter 7 supplies the equilibrium liquid to the waste liquid pipe 6 through outlet pipe 8 and supplies the raw liquid to the fourth filter 7 through raw liquid pipe 2.

[0037] The first filter 7 delivers the regenerated liquid to the waste liquid pipe 6 through the outlet pipe 8, and then delivers the equilibrium liquid again, repeating the above steps. The second filter 7 delivers the adsorbed material to the collection pipe 5 through the outlet pipe 8, and then delivers the regenerated liquid for regeneration. After the fourth filter 7 completes the reaction, it discharges the original liquid after the reaction is complete. The third filter 7 delivers the equilibrium liquid to the fourth filter 7 as needed, and then starts delivering the eluent through the eluent pipe 3.

[0038] The second cycle then proceeds according to the steps outlined above. The second, third, and fourth filters (7 and 7) are executed sequentially according to the order of the first stage, forming a closed-loop production operation. Depending on the specific production conditions, the adsorption separation packing is regenerated at the end of each production cycle to maintain a stable adsorption capacity. This process can continuously process at least 3 tons of urine per day in a closed-loop operation, minimizing air pollution. If it is necessary to increase the daily urine processing volume, the capacity of each individual unit can be increased, multiplying the capacity of the cascade adsorption separation unit, or the number of individual units can be increased.

[0039] Example 3

[0040] Unlike embodiment 2, there is more than one filter 7, and a connector (18) is provided in the middle of the outer column tube (14). N baffles (16) are provided on the connector (18). The baffles (16) divide the outer column tube (14) into N+1 intervals. An inner column tube (15) is provided in each interval. N is a natural number greater than or equal to 1. The filter 7 is connected through the liquid outlet tube 8, and two adjacent liquid outlet tubes 8 are connected to each other. It also includes multiple filters 7, balance liquid tube 1, raw liquid tube 2, eluent tube 3 and regenerated liquid tube 4. The raw liquid tube 2, eluent tube 3 and regenerated liquid tube 4 are connected to the filter 7 through multiple conveying tubes 9. In use, multiple filters 7 with baffles (16) are connected in series. While increasing the space of the filter 7, the production capacity is expanded. This expands the production scale. At the same time, connecting multiple filters 7 in series and coordinating each step further improves the production efficiency. Furthermore, connecting multiple filters 7 in series can fully improve the utilization efficiency of balance liquid, eluent, etc.

[0041] The above description of the disclosed embodiments enables those skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A novel production scale-up adsorption separation device, characterized in that: Includes a filter (7), the filter (7) includes an outer column tube (14), an inner column tube (15) is provided inside the outer column tube (14), and holes are provided inside the inner column tube (15). The filter (7) is one or more. When there is one filter (7), a connector (18) is provided in the middle of the outer column tube (14), and N baffles (16) are provided on the connector (18). The baffles (16) divide the outer column tube (14) into N+1 intervals, and an inner column tube (15) is provided in each interval. N is a natural number greater than or equal to 1. When there is one or more filters (7), the filters (7) are connected through the liquid outlet pipe (8).

2. The novel production scale-up adsorption separation device according to claim 1, characterized in that: A motor (17) is installed above the outer column tube (14). The motor (17) is installed in correspondence with the inner column tube (15). The output shaft of the motor (17) is connected to the inner column tube (15).

3. The novel production scale-up adsorption separation device according to claim 1, characterized in that: The inner column tube (15) is covered with a screen.

4. The novel production scale-up adsorption separation device according to claim 1, characterized in that: When there is one or more filters (7), a connector (18) is provided in the middle of the outer column tube (14), and N baffles (16) are provided on the connector (18). The baffles (16) divide the outer column tube (14) into N+1 intervals, and an inner column tube (15) is provided in each interval. N is a natural number greater than or equal to 1.

5. The novel production scale-up adsorption separation device according to claim 1, characterized in that: It also includes a balance liquid pipe (1), a raw liquid pipe (2), an eluent pipe (3) and a regenerated liquid pipe (4). The raw liquid pipe (2), the eluent pipe (3) and the regenerated liquid pipe (4) are connected to the filter (7) through a delivery pipe (9). The outlet of the delivery pipe (9) is connected to an outlet pipe (8). The outlet pipe (8) is connected in sequence to a liquid pump (11), a first valve (12) and a second valve (13). The outlet of the liquid pump (11) is connected to the inlet of the filter (7). The filter (7) is connected to the outlet pipe (8).

6. The novel production scale-up adsorption separation device according to claim 5, characterized in that: It also includes a collection pipe (5) and a waste liquid pipe (6). The first outlet of the liquid outlet pipe (8) is connected to the collection pipe (5), and the second outlet of the liquid outlet pipe (8) is connected to the waste liquid pipe (6).

7. The novel production scale-up adsorption separation device according to claim 6, characterized in that: Solenoid valves are installed at the connection points of the collection pipe (5) and the waste liquid pipe (6) with the outlet pipe (8).

8. The novel production scale-up adsorption separation device according to claim 5, characterized in that: The first and second outlets of the liquid outlet pipe (8) are located between the first valve (12) and the second valve (13).

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