microplate reader microplate

Abstract

The utility model relates to enzyme label appearance technical field, and disclose enzyme label appearance microwell plate, including the hole plate that the micro -hole is opened in on it even through, still include: bottom plate, top set up the plate groove for making the hole plate inserts, base plate can be inserted into the plate groove, and the top with the micro -hole on the hole plate corresponding set up the hole bottom structure. The enzyme label appearance microwell plate, through the split type structure design of hole plate, bottom plate and replaceable base plate, make the hole bottom can be replaced for flat bottom, U type bottom, V type bottom or conical flat bottom according to actual experiment demand, improved the function adaptability and universality of microwell plate, reduced the experiment preparation cost.

Description

Technical Field

[0001] This utility model relates to the field of enzyme-linked immunosorbent assay (ELISA) reader technology, specifically to ELISA reader microplates. Background Technology

[0002] An ELISA reader is a commonly used detection device in fields such as immunoassay, enzyme-linked reactions, and cell experiments, and is used in conjunction with microplates. Traditional microplates are usually a one-piece structure, with multiple microwells evenly distributed on a rigid material, each well forming a closed space for sample reaction. Commonly used microplates are generally 96-well plates, and their well bottom structures typically include flat bottoms, U-shaped bottoms, V-shaped bottoms, and conical bottoms.

[0003] In the existing technology, the bottom form of the microplate is fixed during production and cannot be flexibly changed according to different experimental needs (such as optical colorimetry, fluorescence imaging, cell culture, etc.). This leads to increased experimental preparation costs and poor versatility. Moreover, since the microplate is molded as a whole, if some microwells are contaminated or damaged, the whole plate will be scrapped, and the bottom structure cannot be replaced locally. Utility Model Content

[0004] (a) Technical problems to be solved To address the shortcomings of existing technologies, this invention provides a microplate for an enzyme-linked immunosorbent assay (ELISA) reader, which has the advantage of a replaceable well bottom structure.

[0005] (II) Technical Solution To achieve the aforementioned objective of having a replaceable well bottom structure, this utility model provides the following technical solution: a microplate for an ELISA reader, comprising a plate with micropores uniformly and continuously formed thereon, and further comprising: The bottom plate has a groove on the top for inserting the perforated plate; The substrate can be embedded in the plate groove, and the top is provided with a hole bottom structure corresponding to the microholes on the perforated plate.

[0006] As a preferred technical solution of this utility model, the hole bottom structure is a flat bottom, a U-shaped bottom, a V-shaped bottom, or a conical flat bottom.

[0007] As a preferred embodiment of this utility model, an annular groove is formed around the bottom hole structure on the top of the substrate.

[0008] As a preferred embodiment of the present invention, the bottom of the perforated plate is fixedly provided with an annular rubber pad for embedding into the annular groove.

[0009] As a preferred embodiment of this utility model, the side of the base plate is also provided with a pressing mechanism for pressing the perforated plate and the base plate into the plate groove.

[0010] As a preferred technical solution of this utility model, the clamping mechanism includes a screw nut, a pressure plate screw rod, and an adjusting knob; A nut is fixedly installed on the side of the base plate, and a pressure plate screw is screwed to the inner wall of the nut. An adjustment knob is fixedly connected to one end of the pressure plate screw located outside the plate groove. The top of the perforated plate has inclined slopes on both sides for the end of the pressure plate screw to abut against.

[0011] As a preferred embodiment of this utility model, the end of the pressure plate screw located in the plate groove is a spherical surface.

[0012] (III) Beneficial Effects Compared with the prior art, the present invention provides a microplate for an enzyme-linked immunosorbent assay (ELISA) reader, which has the following beneficial effects: 1. The microplate of this microplate reader features a split structure design of well plate, base plate and replaceable substrate, which allows the bottom of the well to be flexibly changed to flat bottom, U-shaped bottom, V-shaped bottom or conical flat bottom according to actual experimental needs, which improves the functional adaptability and versatility of the microplate and reduces experimental preparation costs.

[0013] 2. The microplate of this ELISA reader has a well bottom structure set on the substrate, which corresponds one-to-one with the microwells on the well plate. This makes the well bottom structure standardized, detachable, and easy to replace, thus reducing the cost of experimental consumables.

[0014] 3. The microplate of this microplate reader, through the cooperation of the annular groove and the annular rubber pad, can achieve liquid sealing between the microwell and the bottom structure of the well, prevent sample leakage or contamination between wells, and improve the reliability of experimental data. Attached Figure Description

[0015] Figure 1 This is an exploded view of the present invention; Figure 2 This is a schematic diagram of the overall structure of the present invention; Figure 3 This is a partial cross-sectional view of the substrate of this utility model.

[0016] In the diagram: 1. Perforated plate; 2. Micro-hole; 3. Base plate; 4. Plate groove; 5. Base plate; 6. Hole bottom structure; 7. Annular groove; 8. Annular rubber pad; 9. Sloping surface; 10. Pressure plate screw; 11. Threaded nut; 12. Adjusting knob. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0018] Example: Please see Figures 1-3 A microplate for an ELISA reader, comprising a well plate 1 having microwells 2 uniformly and continuously formed thereon, such as... Figure 1 As shown, the perforated plate 1 can be embedded in the groove 4 of the bottom plate 3; In this embodiment, the substrate 5 is also embedded in the plate groove 4 and located in the lower layer of the perforated plate 1. The top of the substrate 5 is provided with a hole bottom structure 6 corresponding to the microhole 2 on the perforated plate 1. The hole bottom structure 6 can be a flat bottom, a U-shaped bottom, a V-shaped bottom, or a conical flat bottom, etc. The split structure design of the perforated plate 1, the base plate 3 and the replaceable substrate 5 allows the bottom of the perforation to be flexibly replaced with a flat bottom, a U-shaped bottom, a V-shaped bottom or a conical flat bottom according to actual experimental needs, which improves the functional adaptability and versatility of the microplate and reduces the experimental preparation cost. For example, a flat bottom can be used when performing absorbance and fluorescence detection; a U-shaped bottom can be used when performing cell suspension culture; a V-shaped bottom can be used when performing precipitation reactions, sample concentration, collection or transfer; and a conical flat bottom can be used when performing optical detection and efficient drainage experiments.

[0019] like Figure 3 As shown, an annular groove 7 is provided around the bottom structure 6 of the hole on the top of the substrate 5, and an annular pad 8 is fixedly provided at the bottom of the perforated plate 1 for embedding into the annular groove 7. Through the cooperation of the annular groove 7 and the annular pad 8, the liquid seal between the micropore 2 and the bottom structure 6 can be achieved, preventing sample leakage or contamination between pores and improving the reliability of experimental data. Meanwhile, during dismantling, the annular groove 7 also serves to collect liquid, preventing the sample from contaminating the entire plate surface.

[0020] In this embodiment, a pressing mechanism is also provided on the side of the base plate 3. The pressing mechanism is used to press the perforated plate 1 and the substrate 5 into the plate groove 4. Specifically, such as Figure 3As shown, the clamping mechanism includes a screw nut 11, a pressure plate screw 10, and an adjusting knob 12. The screw nut 11 is fixedly installed on the side of the base plate 3. The pressure plate screw 10 is screwed onto the inner wall of the screw nut 11. The adjusting knob 12 is fixedly connected to one end of the pressure plate screw 10 located outside the plate groove 4. The top of the perforated plate 1 has inclined sides forming a surface for the end of the pressure plate screw 10 to abut against the slope surface 9. This end of the pressure plate screw 10 is a spherical surface. When the pressure plate screw 10 is turned by adjusting knob 12, the other end of the pressure plate screw 10 slides down the perforated plate 1 through the slope surface 9, thereby pressing the perforated plate 1 and the base plate 5 into the plate groove 4. In this embodiment, by setting a clamping mechanism, the perforated plate 1 and the substrate 5 can be stably fitted into the plate groove 4, maintaining stable positioning during oscillation culture or automated operation, and preventing loosening or misalignment.

[0021] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microplate for an enzyme-linked immunosorbent assay (ELISA) reader, comprising a well plate (1) having microwells (2) uniformly and continuously perforated thereon, characterized in that, Also includes: The bottom plate (3) has a plate groove (4) on the top for embedding the perforated plate (1); The substrate (5) can be embedded in the plate groove (4), and the top is provided with a hole bottom structure (6) corresponding to the microhole (2) on the perforated plate (1). The hole bottom structure (6) is a flat bottom, a U-shaped bottom, a V-shaped bottom, or a conical flat bottom; The top of the substrate (5) is provided with an annular groove (7) surrounding the hole bottom structure (6); The bottom of the perforated plate (1) is fixedly provided with an annular rubber pad (8) for embedding into the annular groove (7). The side of the base plate (3) is also provided with a pressing mechanism for pressing the perforated plate (1) and the base plate (5) into the plate groove (4).

2. The microplate for an enzyme-linked immunosorbent assay (ELISA) reader according to claim 1, characterized in that: The clamping mechanism includes a screw nut (11), a pressure plate screw rod (10), and an adjusting knob (12). A nut (11) is fixedly installed on the side of the base plate (3). A pressure plate screw (10) is screwed onto the inner wall of the nut (11). An adjustment knob (12) is fixedly connected to one end of the pressure plate screw (10) located outside the plate groove (4). The top of the perforated plate (1) is inclined on both sides to form a ramp surface (9) for the end of the pressure plate screw (10) to abut.

3. The microplate for an enzyme-linked immunosorbent assay (ELISA) reader according to claim 2, characterized in that: The end of the pressure plate screw (10) located in the plate groove (4) is a spherical surface.

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