A fluorescent quantitative PCR instrument adaptive to different size test tubes

Abstract

This utility model discloses a real-time PCR instrument adapted to the placement of test tubes of different sizes. It relates to the technical field of real-time PCR instrument equipment. The instrument includes a main body with a mounting mechanism for test tube detection. The mounting mechanism includes a lifting component and a detection box connected to the main body via a sliding component. Test tubes of different sizes are inserted from top to bottom into a hole composed of a positioning ring and an arc-shaped ring. When the outer wall of the test tube contacts and presses downward against the positioning ring, the positioning ring compresses a spring, causing it to move. The spring's rebound force is converted into a uniform and gentle radial clamping force on the outer wall of the test tube, thus initially adapting to and holding the test tube, achieving initial positioning. The positioning force can automatically adjust according to the size of the test tube, achieving good positioning for test tubes of different diameters, improving the instrument's adaptability to test tubes of different specifications.

Description

Technical Field

[0001] This utility model relates to the technical field of fluorescence quantitative PCR instrument equipment, specifically a fluorescence quantitative PCR instrument that can accommodate test tubes of different sizes. Background Technology

[0002] As a core tool in modern molecular biology and medical diagnostics, quantitative real-time PCR (qPCR) is a technology that adds fluorescent groups to the PCR reaction system and uses the accumulation of fluorescence signals to monitor the entire PCR process in real time, ultimately achieving quantitative analysis of the starting template. This technology has been widely used in gene expression research, pathogen detection, genotyping, and drug development.

[0003] Commercially available quantitative PCR instruments generally have limitations in terms of test tube placement. Most quantitative PCR instruments have a relatively simple test tube placement device design, which can usually only accommodate test tubes of specific sizes. In practical applications, different experimental projects and different detection needs often require test tubes of different diameters and heights. However, existing quantitative PCR instruments cannot flexibly adapt to these different sizes of test tubes, resulting in cumbersome test tube replacement and adaptation operations when using test tubes of different sizes. Therefore, this utility model provides a quantitative PCR instrument that can accommodate test tubes of different sizes. Utility Model Content

[0004] To address the shortcomings of existing technologies, this invention provides a real-time PCR instrument that can accommodate test tubes of different sizes. This solves the problem that existing real-time PCR instruments can only accommodate test tubes of specific sizes. In practical applications, different experimental projects and different detection needs often require test tubes of different diameters and heights. However, existing real-time PCR instruments cannot flexibly adapt to these different sizes of test tubes, resulting in the need for cumbersome test tube replacement and adaptation operations when using test tubes of different sizes.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a real-time fluorescence PCR instrument adaptable to test tubes of different sizes, comprising a main body of the real-time fluorescence PCR instrument, wherein the main body of the real-time fluorescence PCR instrument is provided with a mounting mechanism for test tube detection, the mounting mechanism comprising: The lifting assembly includes a detection box connected by a sliding component inside the main body of the real-time PCR instrument. The detection box has a slide rail bracket evenly fixed inside, and the slide rail bracket has a mounting bracket connected by a threaded component inside. The mounting components include a mounting bracket with a positioning ring connected by an elastic component at its upper end, an arc-shaped ring fixed to the upper end face of the positioning ring, a support plate fixed to the bottom of the detection box, and clamping rings connected by telescopic components on both sides of the support plate.

[0006] Preferably, the sliding assembly includes a groove formed on the inner wall of the main body of the real-time PCR instrument, a sliding rod slidably connected inside the groove, the detection box being fixedly connected to the sliding rod, positioning plates fixed on both side walls of the main body of the real-time PCR instrument, and locking shells fixed on both side walls at one end of the detection box. The locking shells can be inserted into the outer wall of the positioning plates, and bolts are provided inside the positioning plates to connect with the locking shells.

[0007] Preferably, the upper end of the main body of the real-time PCR instrument is fixed with a carrying bracket, and one side of the detection box is a pull rod for pulling.

[0008] Preferably, the threaded assembly includes a screw rotatably connected at the center of the upper end face of the support plate, the mounting bracket is slidably connected inside the slide rail bracket, and the mounting bracket is threadedly connected to the screw.

[0009] Preferably, the elastic component includes a placement hole inside the mounting bracket, fixing plates on both sides of the placement hole, the fixing plates being fixedly connected to the mounting bracket, a spring being fixed to the inner wall of the fixing plates, a positioning ring being fixedly connected to the other end of the spring, and a telescopic sleeve rod being provided inside the spring to ensure stable compression of the spring.

[0010] Preferably, the telescopic assembly includes electric push rods fixed on both sides of the upper end face of the support plate, and the clamping ring is fixedly connected to the telescopic end of the electric push rod.

[0011] Beneficial effects This invention provides a real-time PCR instrument that adapts to the placement of test tubes of different sizes. Compared with the prior art, it has the following advantages: Firstly, this invention allows test tubes of different sizes to be inserted from top to bottom into a hole composed of a positioning ring and an arc-shaped ring. When the outer wall of the test tube contacts and presses down on the positioning ring, the positioning ring compresses the spring, and the spring's rebound force is converted into a uniform and gentle radial clamping force on the outer wall of the test tube, thus initially adapting to and holding the test tube, achieving initial positioning. It can automatically adjust the positioning force according to the size of the test tube, achieving good positioning for test tubes of different diameters, improving the instrument's adaptability to test tubes of different specifications. At the same time, the telescopic sleeve inside the spring ensures stable spring compression, making the positioning more stable. The electric push rods fixed on both sides of the upper end of the support plate are activated, pushing the clamping rings fixedly connected at the telescopic ends of the electric push rods to move towards the test tube. The clamping rings clamp the test tube, further fixing it and preventing it from shaking during the detection process. Simultaneously, the height of the mounting bracket relative to the support plate can be adjusted to accommodate test tubes of different heights, allowing the real-time PCR instrument to hold test tubes of various specifications for detection, improving the instrument's versatility and applicability.

[0012] Secondly, when the test box needs to be pulled out to place the test tube, the operator pulls the lever on one side of the test box. The test box slides in the groove on the inner wall of the main body of the real-time PCR instrument via the sliding rod fixed thereon, thus sliding out the test box. When the test box needs to be moved into the equipment, the lever is pushed. The locking shells on both sides of one end of the test box are inserted into the outer wall of the positioning plate on both sides of the main body of the real-time PCR instrument, and then the locking shells are connected to the positioning plate with bolts to fix the position of the test box. The fixing method of the positioning plate, locking shell, and bolts can provide stable support for the test box and ensure that the test box will not be displaced by external force during the detection process. At the same time, the carrying handle is provided to facilitate picking up and carrying the equipment. Attached Figure Description

[0013] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the locking shell structure of this utility model; Figure 3 This is a schematic diagram of the detection box structure of this utility model; Figure 4 This is a schematic diagram of the support plate structure of this utility model; Figure 5 This is a schematic diagram of the mounting bracket structure of this utility model.

[0014] In the diagram: 1. Main body of the real-time PCR instrument; 2. Detection box; 201. Slide rod; 202. Positioning plate; 203. Locking shell; 3. Pull rod; 301. Hand-held bracket; 4. Slide rail bracket; 401. Support plate; 402. Electric push rod; 403. Clamping ring; 5. Mounting bracket; 501. Screw; 6. Fixing plate; 601. Spring; 602. Positioning ring; 603. Arc ring; 7. Placement hole. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figures 1-5 This utility model provides a technical solution: a real-time PCR instrument adapted to test tubes of different sizes, comprising a main body 1, and a mounting mechanism for test tube detection is provided on the main body 1. The mounting mechanism includes: The lifting assembly includes a detection box 2 connected by a sliding component inside the main body 1 of the real-time PCR instrument. A slide rail bracket 4 is evenly fixed inside the detection box 2. An installation bracket 5 connected by a threaded component is installed inside the slide rail bracket 4. The mounting components include a mounting bracket 5 with a positioning ring 602 connected by an elastic component at its upper end, an arc-shaped ring 603 fixed to the upper end face of the positioning ring 602, a support plate 401 fixed to the bottom of the detection box 2, and clamping rings 403 connected by telescopic components on both sides of the support plate 401.

[0017] In a preferred embodiment, the sliding assembly includes a groove formed on the inner wall of the main body 1 of the real-time PCR instrument. A slide rod 201 is slidably connected inside the groove. The detection box 2 is fixedly connected to the slide rod 201. Positioning plates 202 are fixed on both side walls of the main body 1 of the real-time PCR instrument. Locking shells 203 are fixed on both side walls at one end of the detection box 2. The locking shells 203 can be inserted into the outer wall of the positioning plates 202. Bolts are provided inside the positioning plates 202 to connect with the locking shells 203. A carrying bracket 301 is fixed to the upper end of the main body 1 of the real-time PCR instrument. A pull rod 3 is provided on one side of the detection box 2 for pulling. When it is necessary to pull out the detection box 2 to place the test tube, the operator pulls it. The pull rod 3 on one side of the test box 2 allows the test box 2 to slide out by sliding within the groove on the inner wall of the main body 1 of the real-time PCR instrument via a slide rod 201 fixed thereon. When the test box 2 needs to be moved into the device, simply push the pull rod 3 to insert the locking shells 203 on both sides of one end of the test box 2 into the outer wall of the positioning plates 202 on both sides of the main body 1 of the real-time PCR instrument. Then, use bolts to connect the locking shells 203 to the positioning plates 202 to fix the position of the test box 2. The fixing method of the positioning plates 202, locking shells 203, and bolts can provide stable support for the test box 2 and ensure that the test box 2 will not be displaced due to external forces during the detection process.

[0018] In a preferred embodiment, the threaded assembly includes a screw 501 rotatably connected to the center of the upper end face of the support plate 401. The mounting bracket 5 is slidably connected inside the slide rail bracket 4. The mounting bracket 5 is threadedly connected to the screw 501. When the screw 501 at the center of the upper end face of the support plate 401 is rotated, the mounting bracket 5 moves up and down along the slide rail bracket 4 because it is threadedly connected to the screw 501 and slides inside the slide rail bracket 4. This adjusts the height of the mounting bracket 5 relative to the support plate 401, allowing it to accommodate test tubes of different heights. This enables the real-time PCR instrument to hold test tubes of various sizes for testing, improving the instrument's versatility and applicability.

[0019] In a preferred embodiment, the elastic component includes a placement hole 7 inside the mounting bracket 5, with fixing plates 6 on both sides of the placement hole 7. The fixing plates 6 are fixedly connected to the mounting bracket 5. A spring 601 is fixed to the inner wall of the fixing plate 6, and a positioning ring 602 is fixedly connected to the other end of the spring 601. A telescopic sleeve rod is provided inside the spring 601 to ensure stable compression of the spring 601. The telescopic component includes electric push rods 402 fixed to both sides of the upper end face of the support plate 401, and a clamping ring 403 is fixedly connected to the telescopic end of the electric push rod 402. Test tubes of different sizes are inserted from top to bottom into the hole formed by the positioning ring 602 and the arc-shaped ring 603. When the outer wall of the test tube contacts and presses down on the positioning ring 602, the positioning ring 602 will press down. As the compressed spring 601 moves, the rebound force provided by the spring 601 is converted into a uniform and gentle radial clamping force on the outer wall of the test tube, thereby initially adapting to and clamping the test tube, achieving initial positioning of the test tube. It can automatically adjust the positioning force according to the size of the test tube, and can achieve good positioning for test tubes of different diameters, improving the instrument's adaptability to test tubes of different specifications. At the same time, the telescopic sleeve inside the spring 601 ensures stable compression of the spring 601, making the positioning more stable. The electric push rods 402 fixed on both sides of the upper end face of the support plate 401 are activated, pushing the clamping rings 403 fixedly connected at the telescopic ends of the electric push rods 402 to move towards the test tube. The clamping rings 403 clamp the test tube, further fixing the test tube and preventing the test tube from shaking during the test.

[0020] Additional notes: The positioning ring 602 has a V-shaped structure, which can better adapt to test tubes of different sizes, and the arc-shaped ring 603 can prevent the test tube from colliding with the positioning ring 602 when it is inserted.

[0021] The electric actuator mentioned above can be the XTL100 miniature electric actuator.

[0022] Furthermore, any content not described in detail in this specification is existing technology known to those skilled in the art.

[0023] During operation, when it is necessary to place or retrieve test tubes, the operator first pulls the lever 3 on one side of the test box 2, so that the test box 2 slides in the groove on the inner wall of the main body 1 of the fluorescence quantitative PCR instrument through the slide bar 201 fixed thereon, thereby smoothly sliding the entire test module out of the instrument. Then, the locking shell 203 on both sides of one end of the test box 2 is inserted into the positioning plate 202 on both sides of the main body of the instrument and tightened with bolts, thereby providing stable support for the test box 2. Next, by rotating the screw 501 at the center of the upper end face of the support plate 401, the mounting bracket 5 connected to the internal thread is driven to slide up and down along the slide rail bracket 4 to batch adjust and unify the installation height of all test tubes. After preparation, insert test tubes of different sizes into the hole composed of positioning ring 602 and arc ring 603 from top to bottom; the test tube presses the positioning ring 602, compressing the spring 601 fixed below it by the fixing plate 6. The telescopic sleeve inside the spring 601 ensures stable compression. The resulting adaptive rebound force forms an initial, flexible radial clamping force on the outer wall of the test tube, completing the initial positioning. Finally, activate the electric push rod 402 fixed on both sides of the upper end face of the support plate 401, pushing the clamping ring 403 fixed at its telescopic end to move towards the center, performing a final rigid clamping of the test tube from the side, thereby jointly ensuring the absolute stability of the test tube in the subsequent testing process and preventing shaking.

[0024] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0025] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A real-time PCR instrument adaptable to test tubes of different sizes, comprising a main body (1) of the real-time PCR instrument, characterized in that: The main body (1) of the real-time PCR instrument is provided with a mounting mechanism for tube testing, the mounting mechanism including: The lifting assembly includes a detection box (2) connected by a sliding component inside the main body (1) of the fluorescence quantitative PCR instrument. A slide rail bracket (4) is uniformly fixed inside the detection box (2), and an installation bracket (5) connected by a threaded component is provided inside the slide rail bracket (4). The mounting components include a mounting bracket (5) with a positioning ring (602) connected by an elastic component at its upper end, an arc ring (603) fixed on the upper surface of the positioning ring (602), a support plate (401) fixed at the bottom of the detection box (2), and clamping rings (403) connected by telescopic components on both sides of the support plate (401).

2. The real-time PCR instrument for adapting to different sized test tubes according to claim 1, characterized in that: The sliding assembly includes a groove on the inner wall of the main body (1) of the real-time PCR instrument. A sliding rod (201) is slidably connected inside the groove. The detection box (2) is fixedly connected to the sliding rod (201). Positioning plates (202) are fixed on both sides of the main body (1) of the real-time PCR instrument. A locking shell (203) is fixed on one side of the detection box (2). The locking shell (203) can be inserted into the outer wall of the positioning plate (202). Bolts are provided inside the positioning plate (202) to connect with the locking shell (203).

3. The real-time PCR instrument according to claim 1, adaptable to the placement of test tubes of different sizes, is characterized in that: The upper end of the main body (1) of the real-time PCR instrument is fixed with a carrying bracket (301), and a pull rod (3) is used to pull the detection box (2) on one side.

4. A real-time PCR instrument adaptable to different sized test tubes according to claim 1, characterized in that: The threaded assembly includes a screw (501) rotatably connected at the center of the upper end face of the support plate (401), and the mounting bracket (5) is slidably connected inside the slide rail bracket (4). The mounting bracket (5) is threadedly connected to the screw (501).

5. A real-time PCR instrument adaptable to different sized test tubes according to claim 1, characterized in that: The elastic component includes a placement hole (7) inside the mounting bracket (5), and fixing plates (6) are provided on both sides of the placement hole (7). The fixing plates (6) are fixedly connected to the mounting bracket (5). A spring (601) is fixed on the inner wall of the fixing plate (6). The positioning ring (602) is fixedly connected to the other end of the spring (601). A telescopic sleeve rod is provided inside the spring (601) to ensure stable compression of the spring (601).

6. A real-time PCR instrument adaptable to different sized test tubes according to claim 1, characterized in that: The telescopic assembly includes electric push rods (402) fixed on both sides of the upper end face of the support plate (401), and the clamping ring (403) is fixedly connected to the telescopic end of the electric push rod (402).

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