A device for preparing a serum culture medium for stem cells

Abstract

The utility model discloses a preparation device of serum culture medium special for stem cell, including filter beaker, the bottom fixed mounting of filter beaker has the suction filter pipe, the bottom screw thread connection reagent bottle of suction filter pipe, the lateral wall of suction filter pipe is fixed with the air cock and installed, the utility model discloses when using the device to prepare serum culture medium special for stem cell, now filter beaker bottom adds filter paper, adds all various raw materials required for preparation to filter beaker, then after simple stirring mixes uniformly, through the air pump suction, make the joint space of suction filter pipe and reagent bottle form negative pressure state, and the serum culture medium of mixed configuration passes through filter paper under the action of atmospheric pressure, through this device can the liquid obtained by suction filter directly enter reagent bottle, need not enter ordinary suction filter bottle, in the reagent bottle of transfer, save the configuration step simultaneously, also avoid the inevitable loss caused by the phase storage of reagent in the suction filter bottle.

Description

Technical Field

[0001] This utility model relates to the field of stem cell serum culture medium technology, and in particular to a device for preparing a stem cell-specific serum culture medium. Background Technology

[0002] Stem cells are undifferentiated, immature cells with the potential to regenerate various tissues, organs, and the human body; they are known in the medical field as "universal cells."

[0003] Stem cell culture medium is a special culture environment required for the culture of stem cells to achieve the effect of stem cell adhesion or proliferation.

[0004] Existing equipment for preparing stem cell-specific serum culture media requires filtration after the culture medium is prepared. This filtration process involves physical sterilization, impurity removal, and component stabilization, providing a sterile, clear, and nutritionally balanced growth environment for stem cells. This directly ensures the success rate of cell culture and the reliability of experimental results. However, after filtration, the culture medium solution must be transferred back to the reagent bottle. This process involves cleaning the filtration bottle and dealing with reagent loss during the transfer. Utility Model Content

[0005] The purpose of this invention is to provide an apparatus for preparing a serum culture medium specifically for stem cells, so as to overcome the above-mentioned shortcomings in the prior art.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: a preparation device for a stem cell-specific serum culture medium, comprising a filter beaker, wherein a suction tube is fixedly installed at the bottom of the filter beaker, a reagent bottle is threadedly connected to the bottom of the suction tube, and an air nozzle is fixedly installed on the side wall of the suction tube.

[0007] As a further description of the above technical solution: a honeycomb plate is fixedly installed at the bottom of the filter beaker.

[0008] As a further description of the above technical solution: a baffle is fixedly installed at the bottom of the honeycomb panel, and the connecting end of the air nozzle and the suction pipe is located inside the baffle.

[0009] As a further description of the above technical solution: a rotating component is rotatably connected to the center of the honeycomb panel, a stirring paddle is threadedly connected to the top of the rotating component, and wind shields are fixedly installed on multiple ends of the rotating component.

[0010] As a further description of the above technical solution: the end of the baffle away from the air nozzle is provided with an adapter groove.

[0011] As a further description of the above technical solution: the side wall of the filter tube is provided with anti-slip grooves, and the air nozzle is provided with corrugated grooves.

[0012] As a further description of the above technical solution: the top of the reagent bottle is threaded with a sealing cap.

[0013] This invention provides a device for preparing a serum culture medium specifically for stem cells. It offers the following advantages: When preparing the serum culture medium for stem cells using this device, filter paper is first added to the bottom of a filter beaker. All necessary raw materials are then added to the filter beaker, and after simple stirring and mixing, an air nozzle is connected to an air pump. The air pump draws air, creating a negative pressure in the space between the filtration tube and the reagent bottle. The mixed serum culture medium is then rapidly passed through the filter paper under atmospheric pressure and stored in the reagent bottle. This device allows the filtered liquid to be directly transferred to the reagent bottle, eliminating the need for a conventional filtration flask. This saves preparation steps and avoids the unavoidable loss of reagents during the initial storage in the filtration flask.

[0014] It should be understood that the foregoing general description and the following detailed description are exemplary and illustrative only, and are not intended to limit this disclosure.

[0015] This application provides an overview of various implementations or examples of the technology described in this disclosure, and is not a full disclosure of the entire scope or all features of the disclosed technology. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of a stem cell-specific serum culture medium preparation device proposed in this utility model;

[0017] Figure 2 This is a three-dimensional exploded structural diagram of the present invention;

[0018] Figure 3 This is a three-dimensional structural schematic diagram of the suction filter tube of this utility model;

[0019] Figure 4 This is a top view of the structure of the suction filter tube of this utility model;

[0020] Figure 5 This is a three-dimensional exploded view of the filter beaker of this utility model;

[0021] Figure 6 This is a three-dimensional structural diagram of the reagent bottle of this utility model.

[0022] Legend:

[0023] 1. Filter beaker; 2. Filter tube; 3. Reagent bottle; 4. Air nozzle; 5. Honeycomb plate; 6. Baffle; 7. Rotating component; 8. Stirring paddle; 9. Wind shield; 10. Adaptor groove; 11. Anti-slip groove; 12. Corrugated groove; 13. Sealing cap. Detailed Implementation

[0024] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0025] Reference Figure 1-6 A device for preparing a stem cell-specific serum culture medium includes a filter beaker 1, a suction tube 2 fixedly installed at the bottom of the filter beaker 1, a reagent bottle 3 threadedly connected to the bottom of the suction tube 2, and an air nozzle 4 fixedly installed on the side wall of the suction tube 2. When using this device to prepare the stem cell-specific serum culture medium, filter paper is first added to the bottom of the filter beaker 1, and all the necessary raw materials are added to the filter beaker 1. After simple stirring and mixing, the air nozzle 4 is connected to an air pump. Air is drawn by the air pump, creating a negative pressure state in the space between the suction tube 2 and the reagent bottle 3. The mixed serum culture medium is rapidly passed through the filter paper under atmospheric pressure and enters the reagent bottle 3 for storage. This device allows the liquid obtained from suction filtration to be directly added to the reagent bottle 3, eliminating the need for a regular suction filtration bottle. This saves preparation steps and avoids the unavoidable loss of reagents caused by staged storage in the suction filtration bottle.

[0026] As a preferred technical solution in this embodiment, a honeycomb plate 5 is fixedly installed at the bottom of the filter beaker 1; the honeycomb plate 5 ensures that the liquid can pass smoothly through the filter beaker 1, while also providing good support for the filter paper.

[0027] As a preferred technical solution in this embodiment, a baffle 6 is fixedly installed at the bottom of the honeycomb panel 5, and the connecting end of the air nozzle 4 and the suction pipe 2 is located inside the baffle 6; the baffle 6 isolates the air nozzle 4 from the liquid entering the suction pipe 2, so as to prevent the liquid from being sucked into the air nozzle 4.

[0028] As a preferred technical solution in this embodiment, a rotating component 7 is rotatably connected to the center of the honeycomb plate 5, and a stirring paddle 8 is threadedly connected to the top of the rotating component 7. Multiple ends of the rotating component 7 are fixedly equipped with wind deflectors 9. Since a baffle 6 is fixedly installed at the bottom of the honeycomb plate 5, this baffle 6 forms a specific air duct with the cooperation of the honeycomb plate 5 and the inner wall of the filtration tube 2. When the air nozzle 4 draws air, the airflow rushes entirely into the air duct. The opening of the wind deflector 9 on the rotating component 7 faces the airflow direction. Therefore, when the air nozzle 4 draws air, the wind deflector 9 is pushed by the airflow. The multiple wind deflectors 9 connected to the rotating component 7 continuously provide power to the rotating component 7, thereby causing the stirring paddle 8 to rotate. This ensures that after all the liquid is added to the filter beaker 1, the filtration process automatically stirs the solution, simplifying the operation.

[0029] As a preferred technical solution of this embodiment, the end of the baffle 6 away from the air nozzle 4 is provided with an adapter groove 10; in order to facilitate the contact between the windshield 9 and the airflow, the windshield 9 is set at a position beyond one end of the baffle 6. After the windshield 9 rotates, in order to avoid the collision between the baffle 6 and the windshield 9, an adapter groove 10 is provided on the baffle 6 to facilitate the windshield 9 passing through the baffle 6.

[0030] As a preferred technical solution of this embodiment, the side wall of the filtration tube 2 is provided with an anti-slip groove 11, and the nozzle 4 is provided with a corrugated groove 12; the anti-slip groove 11 facilitates the rotation and separation of the filtration tube 2 and the reagent bottle 3 after the operation is completed, and the corrugated groove 12 on the nozzle 4 makes the connection between the nozzle 4 and the air tube more secure.

[0031] As a preferred technical solution in this embodiment, a sealing cap 13 is threaded onto the top of the reagent bottle 3; after the reagent bottle 3 is filled with serum culture medium, it is sealed and stored by the sealing cap 13.

[0032] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0033] The above description is only a preferred embodiment of the present utility model, but the protection scope of the present utility model is not limited thereto. Any equivalent substitutions or changes made by those skilled in the art within the technical scope disclosed in the present utility model, based on the technical solution and the inventive concept of the present utility model, should be included within the protection scope of the present utility model.

Claims

1. An apparatus for preparing a serum culture medium specifically for stem cells, comprising a filter beaker (1), characterized in that: A suction tube (2) is fixedly installed at the bottom of the filter beaker (1), and a reagent bottle (3) is threadedly connected to the bottom of the suction tube (2). An air nozzle (4) is fixedly installed on the side wall of the suction tube (2).

2. The apparatus for preparing a stem cell-specific serum culture medium according to claim 1, characterized in that, A honeycomb plate (5) is fixedly installed at the bottom of the filter beaker (1).

3. The apparatus for preparing a stem cell-specific serum culture medium according to claim 2, characterized in that, A baffle (6) is fixedly installed at the bottom of the honeycomb panel (5), and the connecting end of the air nozzle (4) and the suction pipe (2) is located inside the baffle (6).

4. The apparatus for preparing a stem cell-specific serum culture medium according to claim 3, characterized in that, The center of the honeycomb panel (5) is rotatably connected to a rotating component (7), and the top of the rotating component (7) is threadedly connected to a stirring paddle (8). Windshields (9) are fixedly installed on multiple ends of the rotating component (7).

5. The apparatus for preparing a stem cell-specific serum culture medium according to claim 4, characterized in that, The baffle (6) has an adapter groove (10) at the end away from the air nozzle (4).

6. The apparatus for preparing a stem cell-specific serum culture medium according to claim 4, characterized in that, The side wall of the filter tube (2) is provided with anti-slip groove (11), and the air nozzle (4) is provided with corrugated groove (12).

7. The apparatus for preparing a stem cell-specific serum culture medium according to claim 4, characterized in that, The reagent bottle (3) is threaded with a sealing cap (13) at the top.

Images