A constant temperature stirring device for biological reaction detection

Abstract

The utility model discloses a constant temperature stirring device for biological reaction detection, including constant temperature oscillation box host computer, the inside of this constant temperature oscillation box host computer is provided with oscillation tray, the support for placing biological reaction container bottle, including mutually perpendicular installation bottom plate and fixed side plate, the steady board of vertical fixed side board top, the moving plate of the sleeve setting in steady board outside, the opposite inboard wall of moving plate is fixedly connected with return spring, and the one end of this return spring is fixedly connected with buffer round block, the installation bottom plate and the shelter pad of design limit the container bottle, avoid the large amplitude deviation of container bottle, through the design steady board and adjusting assembly and oscillation round stick, and moving plate is periodically reciprocating under the inertial force of oscillation, and oscillation stirring rod and oscillation round stick are periodically reciprocating in container bottle, and mix the mixed solution in container bottle, and the application has the stirring limit position design of limiting container bottle and stirring mixed solution.

Description

Technical Field

[0001] This utility model belongs to the technical field of biological experimental stirring devices, specifically relating to a constant temperature stirring device for biological reaction detection. Background Technology

[0002] When conducting bioreaction-related detection experiments in a biological laboratory environment, it is often necessary to keep the reaction solution at a constant temperature and stir it. Uniform mixing needs to be achieved under constant temperature conditions to ensure that the reaction substrate and reagents are in full contact and have uniform concentration, so as to avoid local concentration differences from affecting the detection results. Thermostatic shaking incubator is a device suitable for controlling constant temperature and shaking stirring in biological experiments. Its shaking principle is to use a motor to drive the eccentric wheel to rotate, converting the circular motion into the reciprocating oscillation of the shaking tray. The vibration damping device reduces the vibration impact on the overall equipment. When a constant temperature shaking incubator is used to reciprocate and shake containers holding biological samples and reagents, the containers are placed on the shaking tray. Under the action of vibration, the containers may shift significantly, and the internal solution of the containers will shake greatly, affecting the mixing effect. In addition, if the containers are locked to the shaking tray, the vibration amplitude of the containers is limited, which affects the shaking and stirring effect of the internal solution of the containers. The constant temperature shaking incubator has a stirring limit design for stirring and mixing solutions without limiting the containers, which needs to be improved. Existing constant temperature shaking incubators have the problem of stirring and limiting the solution when used for biological reaction detection without limiting the container. To address this, this application proposes a constant temperature stirring device for biological reaction detection. Utility Model Content

[0003] The purpose of this invention is to provide a constant temperature stirring device for biological reaction detection, so as to solve the problem mentioned in the background art of the lack of a limiting design for the container bottle and the stirring of the mixed solution in the constant temperature shaking incubator.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a constant temperature stirring device for biological reaction detection, comprising... The main unit of the constant temperature shaking chamber is equipped with a shaking tray inside. A support for placing bioreactor flasks includes a mounting base plate and fixed side plates that are perpendicular to each other, a stabilizing plate that is perpendicular to the top of the fixed side plates, and a movable plate that is sleeved on the outside of the stabilizing plate. A return spring is fixedly connected to the inner side wall of the movable plate, and a buffer block is fixedly connected to one end of the return spring. A fixing protrusion is fixedly connected to the inner surface of the movable plate facing the stabilizing plate. A limit block is fixed to the bottom surface of the mounting base plate by screws. The adjustment assembly for the vertical moving plate includes a fixing screw on the bottom surface of the vertical moving plate, a threaded cylinder connected to the bottom end of the fixing screw by a thread, and an oscillating stirring rod at the bottom end of the vertical threaded cylinder.

[0005] Preferably, the surface of the oscillation tray is provided with a recessed limiting slot, and the limiting block is embedded in the corresponding limiting slot.

[0006] Preferably, the mounting base plate has a mounting groove a on its surface, and a uniformly distributed rubber pad is fixedly connected to the inner surface of the mounting base plate in the mounting groove a. The orthographic projection of the rubber pad is a semi-circular plate shape.

[0007] Preferably, partitions are fixedly connected to the surfaces opposite to the stabilizing plate, and the partitions are perpendicular to the stabilizing plate.

[0008] Preferably, the movable plate is a box structure with one open surface, and a T-shaped limiting cylinder runs through the top surface of the movable plate. One end of the limiting cylinder that enters the movable plate is tangent to the outer surface of the partition.

[0009] Preferably, the buffer blocks are distributed on both sides of the stabilizing plate, the distance between the buffer blocks and the stabilizing plate is 1 mm, the fixing protrusion is a semi-cylindrical structure, and the fixing protrusion is tangent to the stabilizing plate.

[0010] Preferably, the outer surface of the threaded cylinder is fitted with a shielding pad, the shielding pad being an inverted "T" shaped structure, the outer surface of the top end of the fixing screw is provided with an external thread, and the outer surface of the bottom end of the fixing screw is smooth.

[0011] Preferably, a through-hole rod is passed through the bottom end of the oscillating stirring rod, and an oscillating rod is vertically connected to both ends of the through-hole rod, the oscillating rod being parallel to the oscillating stirring rod.

[0012] Compared with the prior art, the beneficial effects of this utility model are: 1. In this utility model, the container bottle is restrained by the designed mounting base plate and shielding pad, and the rubber pad and shielding pad limit the container bottle to prevent it from shifting significantly.

[0013] 2. In this utility model, through the designed stabilizing plate, adjusting components, and oscillating rod, the moving plate moves periodically back and forth under the inertial force of oscillation, and the oscillating stirring rod and oscillating rod move periodically back and forth inside the container bottle to stir the mixed solution inside the container bottle. This application has a stirring limiting design that limits the container bottle and the stirring of the mixed solution. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the structure of this utility model; Figure 2This is a three-dimensional structural diagram of the mounting base plate and the movable plate of this utility model; Figure 3 For the present utility model Figure 2 A cross-sectional view of the movable plate in the AA direction; Figure 4 For the present utility model Figure 2 A side view of the mounting base plate. Figure 5 For the present utility model Figure 2 A cross-sectional structural diagram of the movable plate in the BB direction; In the diagram: 1. Constant temperature shaking chamber main unit; 2. Shaking tray; 4. Moving plate; 5. Adjustment component; 21. Limiting slot; 31. Mounting base plate; 32. Fixed side plate; 33. Stabilizing plate; 34. Limiting block; 41. Limiting cylinder; 42. Fixed protrusion; 51. Fixed screw; 52. Threaded cylinder; 53. Shaking rod; 54. Shielding pad; 61. Through rod; 62. Shaking rod; 71. Return spring; 72. Buffer block; 311. Rubber pad; 331. Partition. Detailed Implementation

[0015] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0016] Please see Figures 1 to 5This utility model provides a technical solution: a constant temperature stirring device for biological reaction detection, including a constant temperature shaking chamber main unit 1. The main unit 1 has a shaking tray 2 inside. When the main unit 1 is running, the shaking tray 2 vibrates. If a volumetric flask containing a mixture of biological reaction samples and reagents is placed on the shaking tray 2, the volumetric flask undergoes periodic reciprocating oscillation under vibration, achieving uniform oscillation within the main unit 1 and ensuring uniform mixing within the volumetric flask. A support for placing the biological reaction containers includes a mutually perpendicular mounting base plate 31 and a fixed side plate 32, a stabilizing plate 33 perpendicular to the top of the fixed side plate 32, and a movable plate 4 sleeved on the outside of the stabilizing plate 33. The mounting base plate 31 is combined and connected to the shaking tray 2, facilitating disassembly of the mounting base plate 31. The volumetric flask is placed on the surface of the mounting base plate 31. A return spring 71 is fixedly connected to the inner wall of the movable plate 4. A buffer block 72 is fixedly connected to one end of the return spring 71. When the shaking tray 2 vibrates periodically, under the inertia of the vibration... The movable plate 4 reciprocates outside the stabilizing plate 33. During movement, the buffer block 72 is constrained by the stabilizing plate 33, and the return spring 71 is compressed and shortened due to inertia. A fixing protrusion 42 is fixedly connected to the inner surface of the movable plate 4 facing the stabilizing plate 33. The stabilizing plate 33 constrains the fixing protrusion 42, preventing the movable plate 4 from shaking. The stabilizing plate 33 and the fixing protrusion 42 are in line contact, resulting in low friction and not affecting the reciprocating movement of the movable plate 4. A limit block 34 is fixed to the bottom surface of the mounting base plate 31 by screws. 34 is embedded inside the oscillating tray 2 to define the mounting base plate 31, ensuring accurate installation of the mounting base plate 31; the adjusting component 5 of the vertical moving plate 4 includes a fixing screw 51 on the bottom surface of the vertical moving plate 4, a threaded cylinder 52 connected to the bottom end of the fixing screw 51 by a thread, and an oscillating stirring rod 53 at the bottom end of the vertical threaded cylinder 52. When the moving plate 4 moves periodically back and forth, the adjusting component 5 moves, and the oscillating stirring rod 53 stirs the liquid inside the volumetric flask, ensuring uniform mixing of the liquid inside the volumetric flask.

[0017] In this embodiment, a recessed limiting groove 21 is provided on the surface of the oscillating tray 2, and the limiting block 34 is embedded in the corresponding limiting groove 21. The limiting block 34 is embedded in the interior of the oscillating tray 2, thereby limiting the mounting base plate 31 and ensuring accurate installation of the mounting base plate 31.

[0018] In this embodiment, a mounting groove a is formed on the surface of the mounting base plate 31. A uniformly distributed rubber pad 311 is fixedly connected to the inner surface of the mounting base plate 31 in the mounting groove a. The orthographic projection of the rubber pad 311 is a semi-circular plate shape. The volumetric flask is embedded inside the mounting groove a, and the installation position of the volumetric flask is defined, which facilitates the installation of the volumetric flask. The geometry of the mounting groove a matches the shape of the volumetric flask. The rubber pad 311 plays the role of limiting the volumetric flask and preventing the volumetric flask from shifting over a large range. The rubber pad 311 is elastic and can undergo a certain degree of deformation without affecting the displacement and oscillation of the volumetric flask.

[0019] In this embodiment, partitions 331 are fixedly connected to the surfaces opposite to the stabilizing plate 33. The partitions 331 are perpendicular to the stabilizing plate 33. The movable plate 4 is a box structure with one side open. A T-shaped columnar limiting cylinder 41 penetrates through the top surface of the movable plate 4. One end of the limiting cylinder 41 that penetrates into the movable plate 4 is tangent to the outer surface of the partition 331. The limiting cylinder 41 limits the partition 331. During the periodic reciprocating oscillation of the movable plate 4, the movable plate 4 is limited, preventing the position of the movable plate 4 from being skewed.

[0020] In this embodiment, buffer blocks 72 are distributed on both sides of the stabilizing plate 33, and the distance between the buffer blocks 72 and the stabilizing plate 33 is 1 mm. The fixing protrusion 42 is a semi-cylindrical structure, and the fixing protrusion 42 is tangent to the stabilizing plate 33. The stabilizing plate 33 limits the fixing protrusion 42 to prevent the moving plate 4 from shaking. The stabilizing plate 33 and the fixing protrusion 42 are in line contact, with low friction, which does not affect the reciprocating movement of the moving plate 4.

[0021] In this embodiment, a shielding pad 54 is fitted on the outer surface of the threaded cylinder 52. The shielding pad 54 is an inverted "T" shaped structure. The outer surface of the top end of the fixing screw 51 is provided with external threads, and the outer surface of the bottom end of the fixing screw 51 is smooth. The threaded cylinder 52 can slide at the bottom end of the fixing screw 51. The threaded cylinder 52 and the fixing screw 51 are connected by threaded engagement, which can change the height position of the threaded cylinder 52 and the vibrating stirring rod 53, making it easier to place the volumetric flask on the mounting base plate 31.

[0022] In this embodiment, a through rod 61 is passed through the bottom end of the oscillating stirring rod 53, and an oscillating rod 62 is vertically connected to both ends of the through rod 61. The oscillating rod 62 is parallel to the oscillating stirring rod 53 and acts as a stirring rod. When the moving plate 4 and the adjusting component 5 move back and forth as a whole, the oscillating rod 62 moves accordingly and stirs the solution to ensure that the mixed solution inside the volumetric flask is mixed evenly.

[0023] Working principle and usage process of this utility model: When using a constant temperature shaking chamber, the volumetric flask containing the biological reaction solution is shaken and stirred under constant temperature conditions. The main unit 1 of the constant temperature shaking chamber is running, the shaking tray 2 vibrates, and the volumetric flask containing the mixed solution of biological reaction sample and reagent is placed on the mounting base plate 31. Under vibration, the volumetric flask is subjected to periodic reciprocating oscillation, which achieves the effect of uniform oscillation in the main unit 1 of the constant temperature shaking chamber, ensuring uniform mixing in the volumetric flask. The volumetric flask is embedded in the mounting base plate 31, and the rubber pad 311 restricts the volumetric flask. The installation position of the volumetric flask is limited to avoid large-scale displacement of the volumetric flask. The rubber pad 311 is elastic and can undergo a certain degree of deformation without affecting the displacement and oscillation of the volumetric flask. Manually adjust the position of the threaded cylinder 52 to lower it, so that the vibrating stirring rod 53 and the vibrating rod 62 can be inserted into the container bottle; Manually adjust the position of the shielding pad 54. The shielding pad 54 restricts the top surface of the container bottle to prevent the container bottle from shifting significantly. When the constant temperature oscillation chamber main unit 1 is running, the oscillation tray 2 vibrates, and the mounting base plate 31 and the moving plate 4 are subjected to periodic oscillation. Under the inertial force of the oscillation, the moving plate 4 moves outside the stabilizing plate 33. The vibrating stirring rod 53 and the vibrating round rod 62 move back and forth periodically inside the container bottle to achieve the effect of stirring the mixed solution inside the container bottle; In summary: The constant temperature shaking incubator of this application has a limited container bottle and a stirring limit design for stirring and mixing the solution. The container bottle is embedded in the mounting base plate 31, and the rubber pad 311 and the shielding pad 54 limit the container bottle to prevent large displacement of the container bottle. The moving plate 4 moves back and forth periodically under the inertial force of the oscillation. The shaking stirring rod 53 and the shaking round rod 62 move back and forth periodically in the container bottle to stir the mixed solution in the container bottle.

[0024] Although embodiments of the present invention have been shown and described (see the detailed description above), it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A constant-temperature stirring device for biological reaction detection, characterized in that: include The constant temperature oscillation chamber host (1) has an oscillation tray (2) inside. The support for placing bioreactor bottles includes a mounting base plate (31) and a fixed side plate (32) that are perpendicular to each other, a stabilizing plate (33) that is perpendicular to the top of the fixed side plate (32), and a movable plate (4) that is sleeved on the outside of the stabilizing plate (33). A return spring (71) is fixedly connected to the inner side wall opposite to the movable plate (4). A buffer block (72) is fixedly connected to one end of the return spring (71). A fixing protrusion (42) is fixedly connected to the inner surface of the movable plate (4) facing the stabilizing plate (33). A limit block (34) is fixed to the bottom surface of the mounting base plate (31) by screws. The adjustment assembly (5) of the vertical moving plate (4) includes a fixing screw (51) on the bottom surface of the vertical moving plate (4), a threaded cylinder (52) connected to the bottom end of the fixing screw (51) by a thread, and an oscillating stirring rod (53) at the bottom end of the vertical threaded cylinder (52).

2. The isothermal stirring device for biological reaction detection according to claim 1, characterized in that: The surface of the oscillating tray (2) is provided with a recessed limiting slot (21), and the limiting block (34) is embedded in the corresponding limiting slot (21).

3. The isothermal stirring device for biological reaction detection according to claim 1, characterized in that: The mounting base plate (31) has a mounting groove a on its surface. The mounting base plate (31) has a uniformly distributed rubber pad (311) fixedly connected to the inner surface of the mounting groove a. The orthographic projection of the rubber pad (311) is a semi-circular plate shape.

4. The isothermal stirring device for biological reaction detection according to claim 1, characterized in that: Each of the surfaces opposite to the stabilizing plate (33) is fixedly connected to a partition plate (331), and the partition plate (331) is perpendicular to the stabilizing plate (33).

5. The isothermal stirring device for biological reaction detection according to claim 4, characterized in that: The movable plate (4) is a box structure with one side surface open. A "T"-shaped columnar limiting cylinder (41) runs through the top surface of the movable plate (4). One end of the limiting cylinder (41) that enters the movable plate (4) is tangent to the outer surface of the partition (331).

6. The isothermal stirring device for biological reaction detection according to claim 1, characterized in that: The buffer blocks (72) are distributed on both sides of the stabilizing plate (33). The distance between the buffer blocks (72) and the stabilizing plate (33) is 1 mm. The fixing protrusion (42) is a semi-cylindrical structure and is tangent to the stabilizing plate (33).

7. The isothermal stirring device for biological reaction detection according to claim 1, characterized in that: The outer surface of the threaded cylinder (52) is fitted with a shielding pad (54), which is an inverted "T" shaped structure. The top outer surface of the fixing screw (51) is provided with an external thread, and the bottom outer surface of the fixing screw (51) is smooth.

8. The isothermal stirring device for biological reaction detection according to claim 1, characterized in that: A through rod (61) runs through the bottom end of the oscillating stirring rod (53), and an oscillating rod (62) is vertically connected to both ends of the through rod (61). The oscillating rod (62) is parallel to the oscillating stirring rod (53).

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