Reaction vessel sealing device for dye method cyp enzyme induction studies

Abstract

The utility model relates to chemical reaction appliance technical field discloses the reaction container sealing device for dye method CYP enzyme induction research, including bottle body, the outer wall top of bottle body installs the closed mechanism, the closed mechanism is used for the inside of convenient closed equipment, prevents the inside of equipment from leaking, the outer wall right side of bottle body installs the sampling mechanism, the sampling mechanism is used for the sampling and charging of equipment, reduces the reaction of equipment inside material and outside, the closed mechanism includes the central axis, the central axis sets up in the outer wall top of bottle body. In the utility model, after putting the material to be researched into the inner wall of bottle body, the central axis is inserted into the bottle mouth in alignment, the outer shell is rotated to make the curved groove move, the sliding block is moved along the sliding groove, the sliding groove prevents the displacement of sliding block, the telescopic shaft is extended from the inner wall of central axis, the inside of bottle body is closed, the rubber ring enhances the air tightness to make the sealing pressure of container, the degree of fit consistent reduce the influence on data.

Description

Technical Field

[0001] This utility model relates to the field of chemical reaction apparatus technology, and in particular to a sealing device for reaction containers used in dye-based CYP enzyme induction research. Background Technology

[0002] In dye-based CYP enzyme induction studies, the reaction system needs to maintain a constant temperature, constant humidity, and stable gas composition in a sealed environment: CYP enzyme activity is sensitive to temperature and humidity, and environmental fluctuations will directly affect enzyme induction efficiency and detection repeatability. Volatile substances may be generated during the reaction process, requiring the introduction of specific gases. If the seal is not tight, it will lead to component leakage and infiltration of outside air, interfering with the accuracy of dye signal detection.

[0003] Existing sealing devices mostly use rubber stoppers with wire fixation and ordinary threaded seals. A high-purity silicone sealing ring is embedded inside the sealing cap to form a tight fit with the outer wall of the container opening. However, the existing reaction container sealing devices used for dye-based CYP enzyme induction research cannot guarantee that the sealing pressure and fit of each container are completely consistent by screwing in the threads, resulting in low operational efficiency and affecting the final experimental data. Utility Model Content

[0004] To overcome the above shortcomings, this invention provides a reaction vessel sealing device for dye-based CYP enzyme induction research, aiming to improve the problem of inconsistent equipment sealing in the prior art.

[0005] To achieve the above objectives, this utility model adopts the following technical solution: a sealing device for a reaction vessel used in dye-based CYP enzyme induction research, comprising a bottle body, a sealing mechanism installed at the top of the outer wall of the bottle body, the sealing mechanism being used to conveniently seal the inside of the equipment and prevent internal leakage, a sampling mechanism installed on the right side of the outer wall of the bottle body, the sampling mechanism being used for sampling and feeding of the equipment, reducing the reaction between the internal materials and the external environment, the sealing mechanism comprising a central shaft, the central shaft being disposed at the top of the outer wall of the bottle body, a rotating handle being fixedly connected to the top of the outer wall of the central shaft, a curved groove being formed in the middle of the front side of the outer wall of the central shaft, a shell being rotatably connected to the middle of the outer wall of the central shaft, a sliding groove being formed in the middle of the front side of the inner wall of the shell, a telescopic shaft being slidably connected to the middle of the inner wall of the central shaft, a sliding block being fixedly connected to the middle of the front side of the outer wall of the telescopic shaft, and rubber rings being fixedly connected at equal intervals to the bottom of the outer wall of the telescopic shaft.

[0006] As a further description of the above technical solution:

[0007] The sampling mechanism includes a sampling port located on the lower right side of the outer wall of the bottle. A discharge pipe is connected to the right side of the outer wall of the sampling port. A sampling tube is connected to the middle of the bottom end of the outer wall of the discharge pipe. A squeeze plug is slidably connected to the right end of the inner wall of the discharge pipe. A filter screen is fixedly connected to the lower middle part of the inner wall of the sampling tube. A tube plug is slidably connected to the lower end of the inner wall of the sampling tube. An inlet is located on the upper right side of the outer wall of the bottle. An inlet pipe is connected to the right side of the outer wall of the inlet. An inlet tube is connected to the middle of the top end of the outer wall of the inlet pipe. Another squeeze plug is slidably connected to the inner wall of the inlet pipe. Another filter screen is fixedly connected to the upper middle part of the inner wall of the inlet tube. Another tube plug is slidably connected to the top end of the inner wall of the inlet tube.

[0008] As a further description of the above technical solution:

[0009] An identification plate is fixedly connected to the middle of the front side of the outer wall of the bottle.

[0010] As a further description of the above technical solution:

[0011] The bottle has a scale line on the left side of its outer wall, and the scale line is symmetrically arranged on the left side of the bottle's outer wall.

[0012] As a further description of the above technical solution:

[0013] The bottle has an opening at the top of its outer wall.

[0014] As a further description of the above technical solution:

[0015] A tray is provided at the bottom of the outer wall of the bottle.

[0016] As a further description of the above technical solution:

[0017] The bottom of the outer wall of the tray is fixedly connected to multiple support frames.

[0018] As a further description of the above technical solution:

[0019] A rubber pad is fixedly connected to the bottom of the outer wall of the support frame.

[0020] This utility model has the following beneficial effects:

[0021] 1. In this utility model, after the material to be studied is placed into the inner wall of the bottle, the central shaft is inserted with the bottle mouth aligned. The outer shell is rotated to move the curved groove, which drives the sliding block to move along the sliding groove. The sliding groove prevents the sliding block from shifting, allowing the telescopic shaft to extend from the inner wall of the central shaft, sealing the inside of the bottle. The rubber ring enhances the airtightness, thereby ensuring that the sealing pressure and fit of the container are consistent, reducing the impact on the data.

[0022] 2. In this invention, when the equipment is used for sealed cultivation of materials, the plug inside the feed tube can be pulled out to add materials. After being filtered through a filter screen, the materials are injected into the feed tube and flow into the inlet tube. Then, the squeeze plug is pushed into the inlet tube to press the materials into the bottle. When sampling is required, the squeeze plug inside the outlet tube is pulled out, and the material is extracted by negative pressure adsorption. The plug is then pulled out to take the sample. This facilitates sampling of the equipment without the need for frequent opening of the equipment, thus reducing the risk of infection from the outside environment. Attached Figure Description

[0023] Figure 1 This is a front view of the reaction vessel sealing device for dye-based CYP enzyme induction research proposed in this utility model;

[0024] Figure 2 This is a perspective view of the reaction vessel sealing device for dye-based CYP enzyme induction research proposed in this utility model;

[0025] Figure 3 This is a side view of the reaction vessel sealing device for dye-based CYP enzyme induction research proposed in this utility model;

[0026] Figure 4 This is a partial structural schematic diagram of the sealing device for the reaction vessel used in dye-based CYP enzyme induction research proposed in this utility model;

[0027] Figure 5 This is a schematic diagram of the sealing device for the reaction vessel used in dye-based CYP enzyme induction research proposed in this utility model.

[0028] Legend:

[0029] 1. Bottle body; 2. Sealing mechanism; 201. Central shaft; 202. Rotating handle; 203. Curved groove; 204. Outer shell; 205. Sliding groove; 206. Telescopic shaft; 207. Sliding block; 208. Rubber ring; 3. Sampling mechanism; 301. Sampling port; 302. Discharge pipe; 303. Sampling tube; 304. Tube plug; 305. Filter screen; 306. Squeeze plug; 307. Feed inlet; 308. Feed pipe; 309. Feed pipe; 4. Bottle mouth; 5. Scale line; 6. Identification plate; 7. Tray; 8. Support frame; 9. Rubber pad. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Reference Figure 1 , Figure 3 and Figure 4 This utility model provides an embodiment of a reaction vessel sealing device for dye-based CYP enzyme induction research, comprising a bottle body 1, a sealing mechanism 2 installed at the top of the outer wall of the bottle body 1, the sealing mechanism 2 being used to conveniently seal the interior of the device and prevent leakage, and a sampling mechanism 3 installed on the right side of the outer wall of the bottle body 1, the sampling mechanism 3 being used for sampling and feeding of materials, reducing the reaction between the internal materials and the external environment, and the sealing mechanism 2 including a central shaft 201, the central shaft 201 being disposed at the top of the outer wall of the bottle body 1, the top of the outer wall of the central shaft 201 being fixed. A rotating handle 202 is fixedly connected to the central shaft 201. A curved groove 203 is opened in the middle of the front side of the outer wall of the central shaft 201. A housing 204 is rotatably connected to the middle of the outer wall of the central shaft 201. A sliding groove 205 is opened in the middle of the front side of the inner wall of the housing 204. A telescopic shaft 206 is slidably connected to the middle of the inner wall of the central shaft 201. A sliding block 207 is fixedly connected to the middle of the front side of the outer wall of the telescopic shaft 206. Rubber rings 208 are fixedly connected at equal intervals to the bottom of the outer wall of the telescopic shaft 206. A bottle mouth 4 is opened at the top of the outer wall of the bottle body 1. A tray 7 is provided at the bottom of the outer wall of the bottle body 1.

[0032] Specifically, after placing the material to be studied into the inner wall of the bottle body 1, the lower end of the central shaft 201 is aligned with the center of the bottle mouth 4, and then the central shaft 201 is inserted into the bottle mouth 4; then the outer shell 204 is rotated, and the curved groove 203 on the inner wall of the outer shell 204 rotates and moves synchronously with the outer shell 204; during the movement of the curved groove 203, its groove wall contacts the end of the sliding block 207 and generates a thrust, causing the sliding block 207 to move along the inner wall of the sliding groove 205 on the side wall of the central shaft 201; the sliding groove 205 acts on the sliding block 207. The movement trajectory of block 207 forms a constraint, ensuring that the sliding block 207 moves only radially and does not deviate. When the sliding block 207 moves, it drives the telescopic shaft 206 connected to it to extend synchronously out of the inner wall of the central shaft 201 until the end of the telescopic shaft 206 is in close contact with the inner wall of the bottle body 1, forming a seal for the interior of the bottle body 1. The rubber ring 208 at the end of the telescopic shaft 206 deforms under the action of contact pressure, filling the gap between the telescopic shaft 206 and the inner wall of the bottle body 1, enhancing the airtightness of the interior of the bottle body 1.

[0033] Reference Figure 1 , Figure 2 and Figure 5The sampling mechanism 3 includes a sampling port 301, which is located on the lower right side of the outer wall of the bottle body 1. A discharge pipe 302 is connected to the right side of the outer wall of the sampling port 301. A sampling pipe 303 is connected to the middle of the bottom end of the outer wall of the discharge pipe 302. A squeeze plug 306 is slidably connected to the right end of the inner wall of the discharge pipe 302. A filter screen 305 is fixedly connected to the lower middle part of the inner wall of the sampling pipe 303. A pipe plug 304 is slidably connected to the lower end of the inner wall of the sampling pipe 303. An inlet is located on the upper right side of the outer wall of the bottle body 1. The feed inlet 307 has a feed pipe 309 connected to the right side of its outer wall. The feed pipe 308 is connected to the top middle of the outer wall of the feed pipe 309. Another extrusion plug 306 is slidably connected to the inner wall of the feed pipe 309. Another filter screen 305 is fixedly connected to the upper middle part of the inner wall of the feed pipe 308. Another pipe plug 304 is slidably connected to the top of the inner wall of the feed pipe 308. Multiple support frames 8 are fixedly connected to the bottom of the outer wall of the tray 7. A rubber pad 9 is fixedly connected to the bottom of the outer wall of the support frame 8.

[0034] Specifically, after the equipment is sealed, when it is necessary to add material to the equipment during the material cultivation process, the plug 304 is pulled out from the inner wall of the feed pipe 308, opening the channel of the feed pipe 308; the material to be added is poured into the feed pipe 308, and the material flows through the filter screen 305 inside the feed pipe 308. The filter screen 305 filters impurities in the material, and the filtered material flows into the feed pipe 309; then the squeeze plug 306 is pushed, causing the squeeze plug 306 to extend along the inner wall of the feed pipe 309. Pressure is applied to the material in the feed tube 309 by the squeeze plug 306, squeezing the material into the inside of the bottle 1; when it is necessary to sample and test the material in the culture, the squeeze plug 306 inside the discharge tube 302 is pulled, and the squeeze plug 306 moves to create a negative pressure in the discharge tube 302. The negative pressure adsorbs and extracts the material inside the bottle 1 into the discharge tube 302; then the tube plug 304 at the end of the discharge tube 302 is pulled out, and the material sample is taken out from the discharge tube 302 to complete the sampling.

[0035] Reference Figure 1 , Figure 2 and Figure 3 A sign 6 is fixedly connected to the middle of the front side of the outer wall of the bottle body 1. A scale line 5 is provided on the left side of the outer wall of the bottle body 1. The scale line 5 is symmetrically designed and located on the left side of the outer wall of the bottle body 1.

[0036] Specifically, the label 6 marks the condition and data inside the bottle 1 to facilitate subsequent experiments and the recording of usage. The scale line 5 facilitates the observation of the material inside the bottle 1, making the use and measurement of the equipment easier.

[0037] Working principle: After placing the material to be studied on the inner wall of the bottle body 1, align the central shaft 201 with the bottle mouth 4 and insert the central shaft 201 into the bottle mouth 4. Then rotate the outer shell 204 to move the curved groove 203. The movement of the curved groove 203 allows the sliding block 207 to move on the inner wall of the sliding groove 205. Because of the sliding groove 205, the sliding block 207 will not be displaced. The movement of the sliding block 207 allows the telescopic shaft 206 to extend out of the inner wall of the central shaft 201. The extension of the telescopic shaft 206 seals the inside of the bottle body 1. The rubber ring 208 enhances the airtightness of the inside of the bottle body 1.

[0038] When the equipment is sealed and materials are being cultivated, if it is necessary to add materials inside the equipment, the tube plug 304 is pulled out from the inner wall of the feed pipe 308, and the material to be added is filtered through the filter screen 305 and injected into the feed pipe 308, so that the material flows into the feed pipe 309. Then, the squeeze plug 306 is pushed into the feed pipe 309, and the squeeze plug 306 squeezes the material into the bottle 1. When it is necessary to sample and test the material, the squeeze plug 306 in the discharge pipe 302 is pulled to draw out the material in the bottle 1 by negative pressure adsorption, and then the tube plug 304 is pulled out for sampling.

[0039] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A reaction vessel sealing device for dye-based CYP enzyme induction studies, comprising a bottle body (1), characterized in that: A sealing mechanism (2) is installed on the top of the outer wall of the bottle (1). The sealing mechanism (2) is used to conveniently seal the inside of the equipment to prevent leakage inside the equipment. A sampling mechanism (3) is installed on the right side of the outer wall of the bottle (1). The sampling mechanism (3) is used for sampling and feeding of the equipment to reduce the reaction between the materials inside the equipment and the outside. The sealing mechanism (2) includes a central shaft (201), which is located on the top of the outer wall of the bottle (1). A rotating handle (202) is fixedly connected to the top of the outer wall of the central shaft (201). A curved groove (203) is provided in the middle of the front side of the outer wall of the central shaft (201). A shell (204) is rotatably connected to the middle of the outer wall of the central shaft (201). A sliding groove (205) is provided in the middle of the front side of the inner wall of the shell (204). A telescopic shaft (206) is slidably connected to the middle of the inner wall of the central shaft (201). A sliding block (207) is fixedly connected to the middle of the front side of the outer wall of the telescopic shaft (206). Rubber rings (208) are fixedly connected at equal intervals to the bottom of the outer wall of the telescopic shaft (206).

2. The sealing device for the reaction vessel used in dye-based CYP enzyme induction research according to claim 1, characterized in that: The sampling mechanism (3) includes a sampling port (301), which is located on the lower right side of the outer wall of the bottle (1). A discharge pipe (302) is connected to the right side of the outer wall of the sampling port (301). A sampling pipe (303) is connected to the middle of the bottom end of the outer wall of the discharge pipe (302). A squeeze plug (306) is slidably connected to the right end of the inner wall of the discharge pipe (302). A filter screen (305) is fixedly connected to the lower middle part of the inner wall of the sampling pipe (303). A tube is slidably connected to the lower end of the inner wall of the sampling pipe (303). The bottle body (1) has an inlet (307) on the upper right side of the outer wall of the bottle body (1). The inlet (307) is connected to the inlet pipe (309) on the right side of the outer wall of the inlet (307). The inlet pipe (308) is connected to the top center of the outer wall of the inlet pipe (309). Another squeeze plug (306) is slidably connected to the inner wall of the inlet pipe (309). Another filter screen (305) is fixedly connected to the upper center of the inner wall of the inlet pipe (308). Another pipe plug (304) is slidably connected to the top of the inner wall of the inlet pipe (308).

3. The reaction vessel sealing device for dye-based CYP enzyme induction research according to claim 1, characterized in that: A sign (6) is fixedly connected to the middle of the front side of the outer wall of the bottle (1).

4. The sealing device for the reaction vessel used in dye-based CYP enzyme induction research according to claim 1, characterized in that: The bottle body (1) has a scale line (5) on the left side of its outer wall. The scale line (5) is symmetrically arranged on the left side of the outer wall of the bottle body (1).

5. The sealing device for the reaction vessel used in dye-based CYP enzyme induction research according to claim 1, characterized in that: The bottle body (1) has a bottle opening (4) at the top of its outer wall.

6. The reaction vessel sealing device for dye-based CYP enzyme induction research according to claim 1, characterized in that: A tray (7) is provided at the bottom of the outer wall of the bottle (1).

7. The sealing device for the reaction vessel used in dye-based CYP enzyme induction studies according to claim 6, characterized in that: The bottom of the outer wall of the tray (7) is fixedly connected to multiple support frames (8).

8. The sealing device for the reaction vessel used in dye-based CYP enzyme induction studies according to claim 7, characterized in that: A rubber pad (9) is fixedly connected to the bottom of the outer wall of the support frame (8).

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