An ultrasonic-assisted selenoprotein extraction device

Abstract

The utility model relates to selenoprotein extraction technical field provides an ultrasonic auxiliary selenoprotein extraction device, including water tank and ultrasonic assembly, the bottom of water tank is provided with ultrasonic assembly, one side of water tank is provided with the lifting structure. The utility model discloses cooling structure is provided, through the flange plate external cooling liquid, makes the internal circulation flow of cooling liquid through liquid inlet pipe and enters the cooling pipe, absorbs the heat generated by ultrasonic, under the action of spoiler, the flow of cooling liquid is slowed down, and the heat exchange efficiency is improved, and the cooling liquid after heat absorption is discharged through the liquid discharge pipe and completes the cooling, and the temperature of water in the water tank is monitored in real time through the temperature sensor, when the temperature is too high, the temperature sensor starts work through the singlechip control switch valve, makes the cooling liquid circulation cooling, thereby ensuring that the temperature in the extraction tank is stable in the selenoprotein activity suitable interval, realizes the function that the device is convenient for cooling, thereby improved the applicability of the ultrasonic auxiliary selenoprotein extraction device when using.

Description

Technical Field

[0001] This utility model relates to the field of selenoprotein extraction technology, and in particular to an ultrasound-assisted selenoprotein extraction device. Background Technology

[0002] Selenogens are a special type of protein containing selenium. Selenium is embedded in the protein structure in the form of selenocysteine, becoming a key component of its active center. In the process of extracting selenoproteins, the cavitation effect of ultrasound and mechanical vibration are needed to accelerate the release of selenoproteins from the raw material into the solvent, so an ultrasound-assisted selenoprotein extraction device is used.

[0003] To this end, patent CN213232077U discloses an ultrasonic-assisted protein extraction device, belonging to the field of protein extraction technology. This ultrasonic-assisted protein extraction device includes an extraction chamber, a pressurizing device, and an extraction assembly. The extraction chamber includes a chamber body and an electric heating plate. A support column is fixed to the bottom of the chamber body, an ultrasonic oscillator is installed at the bottom of the chamber body, and the electric heating plate is embedded in the inner surface of the chamber body. The pressurizing device includes an electric cylinder and a temperature controller. The electric cylinder is installed on the top surface of the chamber body, and its output end penetrates through the chamber body. The extraction assembly includes a discharge pipe and a fixing plate. The discharge pipe penetrates through and is fixed to the bottom of the chamber body. A control valve is installed on one outer surface of the discharge pipe, and the top surface of the fixing plate is in close contact with the bottom of the discharge pipe. This invention allows for convenient centralized collection of proteins during the extraction process, improving collection efficiency.

[0004] While the ultrasound-assisted protein extraction device mentioned above can conveniently collect proteins and improve collection efficiency, it is difficult to cool down the protein during use. Selenoproteins are heat-sensitive biomolecules, and excessively high temperatures can cause them to denature and become inactive, which not only reduces the extraction rate but also destroys their biological activity, leading to a decline in product quality. Utility Model Content

[0005] The purpose of this invention is to provide an ultrasound-assisted selenoprotein extraction device to solve the problem of inconvenient cooling in existing ultrasound-assisted selenoprotein extraction devices.

[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution: an ultrasound-assisted selenoprotein extraction device, comprising a water tank and an ultrasound component;

[0007] An ultrasonic component is installed at the bottom of the water tank, and a lifting structure is installed on one side of the water tank;

[0008] The top of the ultrasonic component is provided with an extraction vessel, and the interior of the extraction vessel is provided with a stirring structure.

[0009] A cooling structure is provided inside the water tank on one side above the ultrasonic component. The cooling structure includes a cooling pipe fixed inside the water tank on one side above the ultrasonic component. A temperature sensor runs through the water tank on the other side above the ultrasonic component. One end of the cooling pipe extends to the outside of the water tank and is fixed with an inlet pipe. The other end of the cooling pipe extends to the outside of the water tank and is fixed with a drain pipe. Baffles are evenly fixed on both sides inside the cooling pipe.

[0010] When using this device, the cooling structure facilitates temperature reduction, thereby improving its applicability; the lifting structure facilitates opening and locking, enhancing its ease of use; and the stirring structure improves extraction efficiency, thus increasing its overall working efficiency.

[0011] Preferably, the inlet pipe and the outlet pipe are each equipped with a switching valve, and a flange is fixed to one end of each pipe. The output of the temperature sensor is electrically connected to the input of the switching valve via a microcontroller. Coolant is connected to the flange, allowing it to circulate inside the cooling pipe through the inlet pipe, absorbing the heat generated by the ultrasound. The flow of the coolant is slowed by the baffle, improving heat exchange efficiency. The cooled coolant is then discharged through the outlet pipe, completing the cooling process.

[0012] Preferably, the lifting structure includes a bracket, an electric push rod, a sliding rod, and a top cover. The bracket is located on one side of the water tank, and the top cover is located on the outer side of the top of the water tank. An electric push rod passes through one side of the top of the bracket, and sliding rods pass through the interior of both sides of the bracket.

[0013] Preferably, the bottom end of the electric push rod is fixedly connected to the top end of the top cover, and the bottom end of the sliding rod is fixedly connected to the top end of the top cover. The sliding rod and the bracket form a sliding structure. When the electric push rod drives the top cover to rise or fall, the stability of the top cover during movement is enhanced by the action of the sliding rod. After movement, the top cover covers the top of the water tank. When the top cover separates from the water tank, it is convenient to remove the extraction tank.

[0014] Preferably, the ultrasonic component includes an inlet pipe, a drain pipe, a partition, and ultrasonic transducers. The partition is fixed to the bottom of the water tank, and ultrasonic transducers are evenly installed on both sides of the bottom end of the partition. A drain pipe is fixed to one side of the bottom end of the partition, and an inlet pipe is fixed to one side of the top of the water tank.

[0015] Preferably, one end of the drain pipe extends to the outside of the water tank, and the top of the partition contacts the bottom of the extraction tank. Compared with methods such as high-temperature extraction, ultrasonic treatment has a shorter processing time, can be performed at low temperatures, and helps protect the bioactivity and structural integrity of selenoproteins, preventing selenium from detaching from the protein.

[0016] Preferably, the stirring structure includes a stirring shaft, stirring blades, a support plate, and a drive motor. The stirring shaft is disposed inside the extraction tank, and stirring blades are uniformly fixed on the outer wall of the stirring shaft. A support plate is fixed to the top of the extraction tank, and a drive motor is mounted on the top of the support plate. When the stirring shaft rotates inside the extraction tank, it drives the stirring blades to stir the materials and solvent inside the extraction tank.

[0017] Preferably, the bottom end of the stirring shaft extends into the interior of the extraction tank and forms a rotating structure with the extraction tank. The top end of the stirring shaft passes through the support plate and is fixedly connected to the output end of the drive motor. The stirring blades are designed with an inclination. By designing the stirring blades with an inclination, the resistance of the stirring blades during rotation is reduced, allowing the material and solvent to mix thoroughly and improving the extraction efficiency.

[0018] The advantages of the ultrasound-assisted selenoprotein extraction device provided by this utility model are as follows:

[0019] By incorporating a cooling structure and connecting coolant externally via a flange, the coolant circulates within the cooling pipe through the inlet pipe, absorbing the heat generated by ultrasound. The flow of the coolant is slowed by a baffle plate, improving heat exchange efficiency. The cooled coolant is then discharged through a drain pipe, completing the cooling process. A temperature sensor monitors the water temperature in the tank in real time. When the temperature is too high, the sensor, controlled by a microcontroller, activates a switching valve to circulate the coolant and lower the temperature, ensuring the temperature inside the extraction tank remains stable within the optimal range for selenoprotein activity. This design facilitates cooling and enhances the applicability of the ultrasound-assisted selenoprotein extraction device.

[0020] With a lifting structure, when the electric push rod drives the top cover to rise and fall, the stability of the top cover during movement is enhanced by the action of the sliding rod. After the top cover is moved, it covers the top of the water tank. When the top cover is separated from the water tank, the extraction tank can be easily removed. This realizes the function of easy opening and fixing of the device, thereby improving the convenience of using the ultrasonic-assisted selenoprotein extraction device.

[0021] By incorporating a stirring structure, when the stirring shaft rotates inside the extraction tank, it drives the stirring blades to stir the material and solvent inside the extraction tank. The inclined design of the stirring blades reduces the resistance during rotation, allowing the material and solvent to mix thoroughly and improving extraction efficiency. This device facilitates improved extraction efficiency and enhances the working efficiency of the ultrasound-assisted selenoprotein extraction device during use. Attached Figure Description

[0022] Figure 1 This is a three-dimensional schematic diagram of the present invention;

[0023] Figure 2 This is a three-dimensional schematic diagram of the main cross-section of this utility model;

[0024] Figure 3 This is a three-dimensional cross-sectional view of the left side of this utility model;

[0025] Figure 4 This is a three-dimensional side view cross-sectional diagram of the stirring structure of this utility model;

[0026] Figure 5 This is a three-dimensional cross-sectional view of the right side of this utility model;

[0027] Figure 6 This is a three-dimensional side view cross-sectional diagram of the cooling structure of this utility model.

[0028] The following are the annotations in the diagram: 1. Water tank; 2. Cooling structure; 21. Temperature sensor; 22. Cooling pipe; 23. Liquid inlet pipe; 24. Liquid outlet pipe; 25. Baffle plate; 3. Lifting structure; 31. Support; 32. Electric push rod; 33. Slide rod; 34. Top cover; 4. Ultrasonic assembly; 41. Water inlet pipe; 42. Drain pipe; 43. Baffle plate; 44. Ultrasonic transducer; 5. Extraction tank; 6. Stirring structure; 61. Stirring shaft; 62. Stirring blade; 63. Support plate; 64. Drive motor. Detailed Implementation

[0029] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0030] Please see Figures 1-6This utility model provides an ultrasound-assisted selenoprotein extraction device, including a water tank 1 and an ultrasound component 4. The ultrasound component 4 is located at the bottom of the water tank 1, and a lifting structure 3 is located on one side of the water tank 1. The lifting structure 3 includes a support 31, an electric push rod 32, a sliding rod 33, and a top cover 34. The support 31 is located on one side of the water tank 1, and the top cover 34 is located on the outer side of the top of the water tank 1. The electric push rod 32 passes through one side of the top of the support 31, and the sliding rod 33 passes through the interior of the support 31 on both sides of the electric push rod 32. The bottom end of the electric push rod 32 is connected to the top of the top cover 34. The bottom end of the slide rod 33 is fixedly connected to the top end of the top cover 34. The slide rod 33 and the bracket 31 form a sliding structure. The ultrasonic component 4 includes a water inlet pipe 41, a drain pipe 42, a partition 43 and an ultrasonic transducer 44. The partition 43 is fixed to the bottom of the water tank 1. Ultrasonic transducers 44 are evenly installed on both sides of the bottom end of the partition 43. A drain pipe 42 is fixed to one side of the bottom end of the partition 43. A water inlet pipe 41 is fixed to one side of the top of the water tank 1. One end of the drain pipe 42 extends to the outside of the water tank 1. The top end of the partition 43 is in contact with the bottom end of the extraction tank 5.

[0031] Reference Figure 1 and Figure 2 As shown, activating the electric push rod 32 causes the top cover 34 to rise and fall. The top cover 34 then moves the sliding rod 33 within the bracket 31. The sliding rod 33 enhances the stability of the top cover 34 during movement. After moving, the top cover 34 covers the top of the water tank 1. When the top cover 34 separates from the water tank 1, the extraction tank 5 can be easily removed, completing the collection of the selenium protein extract. Subsequently, the extract can be separated. Water enters the water tank 1 through the inlet pipe 41, setting the water level at the top of the cooling pipe 22. Under the action of the ultrasonic transducer 44, the ultrasonic signal is converted into high-frequency mechanical vibration. The ultrasonic waves vibrate in the liquid medium... During sound propagation, countless tiny bubbles are generated. These bubbles rapidly expand, contract, and eventually collapse violently under the influence of sound pressure, producing extreme high temperatures, strong shock waves, and microjets in the localized environment. The intense mechanical force can efficiently break the cell walls and cell membranes of selenoprotein-rich plant or animal tissues, allowing for the full release of intracellular selenoproteins. The shock waves and microjets can greatly disturb the interface layer between the solvent and the material, accelerating the diffusion rate of proteins from the solid matrix to the extraction solvent. Compared with high-temperature extraction and other methods, ultrasonic treatment has a shorter processing time and can be carried out at low temperatures, which helps to protect the biological activity and structural integrity of selenoproteins and prevent selenium from detaching from the proteins.

[0032] An extraction tank 5 is provided at the top of the ultrasonic component 4. A stirring structure 6 is provided inside the extraction tank 5. The stirring structure 6 includes a stirring shaft 61, stirring blades 62, a support plate 63, and a drive motor 64. The stirring shaft 61 is located inside the extraction tank 5. Stirring blades 62 are evenly fixed on the outer wall of the stirring shaft 61. A support plate 63 is fixed at the top of the extraction tank 5. A drive motor 64 is installed at the top of the support plate 63. The bottom end of the stirring shaft 61 extends into the interior of the extraction tank 5 and forms a rotating structure with the extraction tank 5. The top end of the stirring shaft 61 passes through the support plate 63 and is fixedly connected to the output end of the drive motor 64. The stirring blades 62 are designed to be inclined.

[0033] Reference Figure 3 and Figure 5 As shown, the material and solvent are poured into the extraction tank 5, and the drive motor 64 is started to make the stirring shaft 61 rotate inside the extraction tank 5. The stirring shaft 61 will drive the stirring blade 62 to stir the material and solvent inside the extraction tank 5. The inclined design of the stirring blade 62 reduces the resistance of the stirring blade 62 when rotating, so that the material and solvent are fully mixed and the extraction efficiency is improved.

[0034] A cooling structure 2 is installed inside the water tank 1 on one side above the ultrasonic component 4. The cooling structure 2 includes a cooling pipe 22 fixed inside the water tank 1 on one side above the ultrasonic component 4. A temperature sensor 21 runs through the water tank 1 on the other side above the ultrasonic component 4. One end of the cooling pipe 22 extends to the outside of the water tank 1 and is fixed with an inlet pipe 23. The other end of the cooling pipe 22 extends to the outside of the water tank 1 and is fixed with a drain pipe 24. Baffles 25 are evenly fixed on both sides inside the cooling pipe 22. Switch valves are respectively installed inside the inlet pipe 23 and the drain pipe 24. A flange is fixed to one end of the inlet pipe 23 and the drain pipe 24. The output end of the temperature sensor 21 is electrically connected to the input end of the switch valve through a microcontroller.

[0035] Reference Figure 2 and Figure 6 As shown, coolant is connected to the flange and circulates inside the cooling pipe 22 through the inlet pipe 23, absorbing the heat generated by ultrasound. Under the action of the baffle 25, the flow of coolant is slowed down, improving the heat exchange efficiency. After absorbing heat, the coolant is discharged through the drain pipe 24 to complete the cooling. The temperature of the water in the water tank 1 is monitored in real time by the temperature sensor 21. When the temperature is too high, the temperature sensor 21 controls the switch valve through the microcontroller to start working, so that the coolant circulates and cools down, thereby ensuring that the temperature inside the extraction tank 5 is stable within the suitable range for selenoprotein activity.

[0036] Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An ultrasound-assisted selenoprotein extraction device, comprising a water tank (1) and an ultrasound component (4); Its features are: An ultrasonic component (4) is provided at the bottom of the water tank (1), and a lifting structure (3) is provided on one side of the water tank (1); The top of the ultrasonic component (4) is provided with an extraction tank (5), and the interior of the extraction tank (5) is provided with a stirring structure (6); A cooling structure (2) is provided inside the water tank (1) on one side above the ultrasonic component (4). The cooling structure (2) includes a cooling pipe (22) fixed inside the water tank (1) on one side above the ultrasonic component (4). A temperature sensor (21) passes through the water tank (1) on the other side above the ultrasonic component (4). One end of the cooling pipe (22) extends to the outside of the water tank (1) and is fixed with an inlet pipe (23). The other end of the cooling pipe (22) extends to the outside of the water tank (1) and is fixed with a drain pipe (24). Baffles (25) are evenly fixed on both sides inside the cooling pipe (22).

2. The ultrasound-assisted selenoprotein extraction device according to claim 1, characterized in that: The inlet pipe (23) and outlet pipe (24) are respectively equipped with switching valves. One end of the inlet pipe (23) and outlet pipe (24) is respectively fixed with a flange. The output end of the temperature sensor (21) is electrically connected to the input end of the switching valve through a microcontroller.

3. The ultrasound-assisted selenoprotein extraction device according to claim 1, characterized in that: The lifting structure (3) includes a bracket (31), an electric push rod (32), a slide rod (33), and a top cover (34). The bracket (31) is located on one side of the water tank (1). The top cover (34) is located on the outer side of the top of the water tank (1). An electric push rod (32) passes through one side of the top of the bracket (31). Slide rods (33) pass through the interior of the bracket (31) on both sides of the electric push rod (32).

4. The ultrasound-assisted selenoprotein extraction device according to claim 3, characterized in that: The bottom end of the electric push rod (32) is fixedly connected to the top end of the top cover (34), and the bottom end of the slide rod (33) is fixedly connected to the top end of the top cover (34). The slide rod (33) and the bracket (31) constitute a sliding structure.

5. The ultrasound-assisted selenoprotein extraction device according to claim 1, characterized in that: The ultrasonic component (4) includes an inlet pipe (41), a drain pipe (42), a partition (43), and an ultrasonic transducer (44). The partition (43) is fixed to the bottom of the water tank (1). Ultrasonic transducers (44) are evenly installed on both sides of the bottom end of the partition (43). A drain pipe (42) is fixed on one side of the bottom end of the partition (43). An inlet pipe (41) is fixed on one side of the top of the water tank (1).

6. The ultrasound-assisted selenoprotein extraction device according to claim 5, characterized in that: One end of the drain pipe (42) extends to the outside of the water tank (1), and the top of the partition (43) contacts the bottom of the extraction tank (5).

7. The ultrasound-assisted selenoprotein extraction device according to claim 1, characterized in that: The stirring structure (6) includes a stirring shaft (61), stirring blades (62), a support plate (63), and a drive motor (64). The stirring shaft (61) is located inside the extraction tank (5). Stirring blades (62) are evenly fixed on the outer wall of the stirring shaft (61). The top of the extraction tank (5) is fixed with a support plate (63), and the top of the support plate (63) is equipped with a drive motor (64).

8. The ultrasound-assisted selenoprotein extraction device according to claim 7, characterized in that: The bottom end of the stirring shaft (61) extends into the interior of the extraction tank (5) and forms a rotating structure with the extraction tank (5). The top end of the stirring shaft (61) passes through the support plate (63) and is fixedly connected to the output end of the drive motor (64). The stirring blade (62) is designed to be inclined.

Images