A cell culture tube

Abstract

The utility model relates to cell culture technical field discloses a cell culture tube. The cell culture tube includes the pipe body, first cover, second cover and hydrophobic gas permeable membrane. One end of the pipe body has the pipe orifice, and the inside of the pipe body is used for cultivating cells, first cover is detachably covered and arranged on the pipe orifice to seal the pipe body, second cover is detachably covered and arranged on the pipe orifice or first cover, and second cover is provided with the gas permeable hole, hydrophobic gas permeable membrane is arranged in second cover, and the gas permeable hole is covered. When sealing transportation, first cover is covered on the pipe body, avoids the problem such as liquid leakage pollution, and second cover is covered on first cover, avoids second cover forgetting loss, when stable cultivation cell, first cover is taken off, and second cover is covered on the pipe body, and the hydrophobic gas permeable membrane in second cover guarantees that the pipe body is sterile and the culture environment of breathable. The cell culture tube can be compatible with sealed transportation and breathable culture, effectively avoid the pollution of cell, improve cell culture quality, and simple operation.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a cell culture tube. Background Technology

[0002] In the field of biomedicine, cell culture is often required when conducting in vitro cell experiments. Disposable sterile culture flasks are generally used as cell culture containers.

[0003] Existing cell culture tubes are equipped with either breathable or sealed caps. However, in certain situations, it is often necessary to transport the cell culture containers in which cells are being cultured. Breathable caps may leak or become contaminated during transport, affecting cell culture and even causing cell culture failure. While sealed caps can avoid problems during transport, they are difficult to maintain the breathable environment required for cell survival, affecting cell culture and even causing cell death.

[0004] Therefore, there is an urgent need for a cell culture tube to solve the above problems. Utility Model Content

[0005] The purpose of this invention is to provide a cell culture tube that can simultaneously accommodate sealed transport and breathable culture, effectively preventing cell contamination, improving cell culture quality, and is simple to operate.

[0006] To achieve this objective, the present invention adopts the following technical solution:

[0007] This invention provides a cell culture tube, comprising:

[0008] A tube, one end of which has an opening, and the interior of which is used for culturing cells;

[0009] A first cover body is detachably installed on the pipe opening to seal the pipe body;

[0010] A second cover is detachably installed on the pipe opening or the first cover, and the second cover is provided with a vent hole.

[0011] A hydrophobic and breathable membrane is disposed in the second cover body and covers the vent hole.

[0012] As an alternative, the first cover includes a sealing part and a connecting part. The sealing part is detachably covered on the pipe opening to seal the pipe body. The connecting part is connected to one axial end of the sealing part, and the second cover is detachably covered on the connecting part.

[0013] As an optional solution, the outer periphery of the sealing part is provided with a first anti-slip structure.

[0014] As an alternative, the outer circumference of the pipe opening is provided with a first external thread, and the inner circumference of the sealing part is provided with a first internal thread that can mate with the first external thread.

[0015] As an alternative, the outer peripheral side of the connecting part is provided with a second external thread, and the inner peripheral side of the second cover is provided with a second internal thread that can mate with the first external thread or the second external thread.

[0016] As an alternative, the outer periphery of the second cover is provided with a second anti-slip structure.

[0017] As an alternative, multiple vent holes are provided, and these multiple vent holes are distributed at intervals on the axial end face of the second cover.

[0018] As an alternative, the vent holes are circular and arranged in a circumferential array.

[0019] As an alternative, the tube is made of a transparent material.

[0020] As an alternative, the tube body is made of PETG material.

[0021] The beneficial effects of this utility model are:

[0022] The cell culture tube provided by this invention features a first cap for sealing and a second cap that allows for ventilation. When sealed transport is required, the first cap is placed on the tube to prevent leakage and contamination. The second cap, placed on top of the first cap, prevents it from being forgotten or lost and reduces the need for additional packaging. When cell culture needs to be stabilized, the first cap is removed, and the second cap is placed on the tube. The hydrophobic and breathable membrane within the second cap ensures a sterile yet breathable culture environment within the tube. This cell culture tube simultaneously supports sealed transport and breathable culture, effectively preventing cell contamination, improving cell culture quality, and is easy to operate. Attached Figure Description

[0023] Figure 1 This is a first-view exploded view of the cell culture tube provided in this embodiment of the present invention.

[0024] Figure 2 This is a second-view exploded view of the cell culture tube provided in this embodiment of the present invention.

[0025] Figure 3 This is a first-view structural schematic diagram of the cell culture tube provided in an embodiment of the present invention;

[0026] Figure 4 This is a schematic diagram of the cell culture tube provided in an embodiment of the present invention from a second perspective.

[0027] Figure 5 This is a schematic diagram of the cell culture tube provided in an embodiment of the present invention from a third-view perspective;

[0028] Figure 6 This is a schematic diagram of the structure of the second cover body covering the pipe body provided in this embodiment of the utility model.

[0029] Figure label:

[0030] 1. Pipe body; 11. Pipe opening; 111. First external thread;

[0031] 2. First cover; 21. Sealing part; 211. First anti-slip structure; 22. Connecting part; 221. Second external thread;

[0032] 3. Second cover; 31. Ventilation hole; 32. Second anti-slip structure; 33. Axial end face;

[0033] 4. Hydrophobic and breathable membrane. Detailed Implementation

[0034] The embodiments of this utility model are described in detail below. Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals denote the same or similar parts or parts having the same or similar functions throughout. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain this utility model, and should not be construed as limiting this utility model.

[0035] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection or a detachable connection; a mechanical connection or an electrical connection; a direct connection or an indirect connection through an intermediate medium; or the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0036] In the description of this utility model, unless otherwise expressly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0037] The following is in conjunction with the appendix Figures 1-6 The technical solution of this utility model will be further illustrated through specific implementation methods.

[0038] like Figures 1 to 4 As shown, this embodiment provides a cell culture tube, including a tube body 1, a first cap 2, a second cap 3, and a hydrophobic and breathable membrane 4. One end of the tube body 1 has a tube opening 11, and the interior of the tube body 1 is used for culturing cells; the first cap 2 is detachably placed on the tube opening 11 to seal the tube body 1; the second cap 3 is detachably placed on the tube opening 11 or the first cap 2, and the second cap 3 is provided with a vent 31; the hydrophobic and breathable membrane 4 is disposed inside the second cap 3 and covers the vent 31, and the hydrophobic and breathable membrane 4 can be locked inside the second cap 3.

[0039] By providing a first cover 2 for sealing and a second cover 3 that allows air to pass through, such as Figure 3 As shown, when sealed transport is required, the first cap 2 is placed over the opening 11 of the tube 1 to seal the tube 1, preventing suspended cells and culture medium from flowing out of the tube 1 when it is tilted, thus avoiding cell contamination. The second cap 3 is placed over the first cap 2 to prevent the second cap 3 from being forgotten or lost, and also reduces the packaging requirements for the second cap 3. Figure 6 As shown, when stable cell culture is required, the second cover 3 with a hydrophobic and breathable membrane 4 is removed from the first cover 2, the first cover 2 is removed from the tube body 1, and then the second cover 3 with the hydrophobic and breathable membrane 4 is placed on the tube body 1. Through the hydrophobic and breathable membrane 4 and the vent 31 on the second cover 3, the cells in the tube body 1 can exchange gases with the external environment, facilitating cell culture. This cell culture tube can simultaneously support sealed transport and breathable culture without replacing the tube body 1, meeting the needs of multiple usage scenarios, effectively preventing cell contamination, improving cell culture quality, and is simple to operate.

[0040] In related technologies, there are also solutions employing two caps, such as the existing patent with publication number CN221397893U, which includes a bottle body, a first cap, and a second cap. The first cap has a through hole and can be placed on the bottle opening. The second cap can be placed on the first cap, and the first cap includes a microporous hydrophobic filter membrane covering the through hole. When the second cap is installed on the first cap, it can provide a completely sealed cap, isolating air and preventing airborne fungi from contaminating the cells in the cell culture container. When the second cap is removed from the first cap, it can also function as a breathable cap, allowing gas exchange between the cell culture container and the external environment to facilitate the culture of certain cells. While this design achieves both sealing and permeability, its sealing mechanism is not conducive to transportation and subsequent culture. During transport, the bottle is prone to tipping, causing the culture medium and even cells to leak through the gaps between the first cap and the microporous hydrophobic membrane into the space between the first and second caps. When the second cap is opened for permeable culture, this can lead to cell contamination and culture failure. In this embodiment, the cell culture tube is sealed by the first cap 2 during transport, without affecting the hydrophobic and permeable membrane 4 inside the second cap 3. Therefore, when stabilizing cell culture, after replacing the second cap 3 onto the tube 1, only the tube opening 11 needs to face upwards. The hydrophobic and permeable membrane 4 inside the second cap 3 ensures a sterile and permeable culture environment within the tube 1.

[0041] like Figure 1 and Figure 2 As shown, the first cover 2 includes a sealing part 21 and a connecting part 22. The sealing part 21 is detachably covered on the tube opening 11 to seal the tube body 1. The connecting part 22 is connected to one axial end of the sealing part 21, and the second cover 3 is detachably covered on the connecting part 22. The sealing part 21 is closed at one end near the connecting part 22, and open at the other end. By setting the sealing part 21 and the connecting part 22, it is possible to achieve that while the sealing part 21 seals the tube opening 11, the second cover 3 can be connected to the connecting part 22 of the first cover 2, without contaminating the inner circumference of the second cover 3 and the hydrophobic and breathable membrane 4 covering the vent 31, thus ensuring the quality of subsequent cell culture.

[0042] like Figures 1 to 2 As shown, the outer circumference of the pipe opening 11 is provided with a first external thread 111, and the inner circumference of the sealing part 21 is provided with a first internal thread (not shown) that can mate with the first external thread 111. When sealing is required, the pipe body 1 can be sealed by the mutual engagement of the first external thread 111 of the pipe body 1 and the first external thread 111 of the sealing part 21. Furthermore, the first cover 2 can be detachably connected to the pipe opening 11 by means of a threaded connection, making disassembly and assembly convenient and quick.

[0043] like Figures 1 to 2As shown, the outer periphery of the connecting part 22 is provided with a second external thread 221, and the inner periphery of the second cover 3 is provided with a second internal thread (not shown) that can mate with the first external thread 111 or the second external thread 221. When sealing is required, the first cover 2 seals at the tube opening 11, and the second internal thread of the second cover 3 and the second external thread 221 of the first cover 2 mate with each other, which can prevent the second cover 3 from being forgotten or lost, reduce the packaging of the second cover 3, and ensure that the second cover 3 is not contaminated. When aeration culture is required, the second cover 3 is removed from the first cover 2, the first cover 2 is removed from the tube 1, and then the second cover 3 is placed on the tube 1. The second internal thread of the second cover 3 and the first external thread 111 of the tube 1 mate with each other, ensuring a sterile environment inside the tube 1. Furthermore, the second cover 3 can be detachably connected to the first cover 2 or the tube 1 through the threaded connection, making disassembly and assembly convenient and quick.

[0044] like Figure 1 and Figure 2 As shown, the hydrophobic and breathable membrane 4 is circular, and its size is basically the same as the internal dimensions of the axial end face 33 of the second cover 3. The hydrophobic and breathable membrane 4 is attached to the axial end face 33 and can be snapped into the second cover 3. During sealing, the connecting part 22 presses against the hydrophobic and breathable membrane 4 located inside the second cover 3, so that the hydrophobic and breathable membrane 4 presses against the axial end face 33 of the second cover 3 to fix the hydrophobic and breathable membrane 4 and ensure that the inside of the hydrophobic and breathable membrane 4 is sterile and uncontaminated. During stable culture, the tube opening 11 presses against the hydrophobic and breathable membrane 4 located inside the second cover 3, so that the hydrophobic and breathable membrane 4 presses against the axial end face 33 of the second cover 3 to fix the hydrophobic and breathable membrane 4 and ensure a sterile environment inside the tube 1.

[0045] like Figure 5 As shown, multiple vent holes 31 are provided, and these vent holes 31 are spaced apart on the axial end face 33 of the second cover 3. The multiple spaced vent holes 31 can increase the area for gas exchange. The larger the area, the greater the air flow per unit time, ensuring the permeability of cultured cells and improving the quality of cell culture.

[0046] like Figure 5 As shown, the vent holes 31 are circular and arranged in a circumferential array. The circular shape of the vent holes 31 facilitates processing. The circumferential array distribution, designed according to the shape of the axial end face 33, achieves symmetrical distribution and ensures uniform gas exchange through different vent holes 31, thereby improving cell culture quality.

[0047] like Figures 1 to 2As shown, a first anti-slip structure 211 is provided on the outer periphery of the sealing part 21. The first anti-slip structure 211 can increase the friction and play an anti-slip role. When installing or removing the first cover 2, the user can easily complete the installation or removal of the first cover 2 by gripping the first anti-slip structure 211 to increase the friction, saving time and effort.

[0048] In this embodiment, the first anti-slip structure 211 can be configured as anti-slip stripes. In other embodiments, the first anti-slip structure 211 can also be configured as a rubber anti-slip sleeve or a textured structure, etc., and no specific limitation is made here.

[0049] like Figures 1 to 2 As shown, a second anti-slip structure 32 is provided on the outer periphery of the second cover 3. The second anti-slip structure 32 can increase the friction and play an anti-slip role. When installing or removing the second cover 3, the user can easily complete the installation or removal of the second cover 3 by gripping the second anti-slip structure 32 to increase the friction, saving time and effort.

[0050] In this embodiment, the second anti-slip structure 32 can be configured as anti-slip stripes. In other embodiments, the second anti-slip structure 32 can also be configured as a rubber anti-slip sleeve or a textured structure, etc., and no specific limitation is made here.

[0051] Optionally, tube 1 is made of a transparent material. The transparency of tube 1 facilitates observation of the culture medium inside. Furthermore, when tube 1 contains a pH indicator, researchers can directly observe the color of the chromogenic agent in the culture medium through the transparent tube 1 to make a preliminary judgment on the cell state.

[0052] Optionally, tube 1 is made of PETG material. PETG material ensures that tube 1 will not react additionally with cells and culture medium in a sterile state without DNA, RNase, or pyrogens, thus ensuring the quality of cell culture. Alternatively, other suitable materials, such as glass, can be selected as needed, without specific limitations.

[0053] It should be noted that the pore size of the hydrophobic and breathable membrane 4 allows gas molecules such as oxygen and carbon dioxide to pass through freely, while effectively blocking the invasion of microorganisms such as bacteria and fungi, thereby preventing contamination. This enables the continuous supply of gas exchange required for cell growth without opening the container, while simultaneously preventing microbial contamination and providing a stable and controllable sterile environment for the cells.

[0054] Optionally, the hydrophobic and breathable membrane 4 is made of polytetrafluoroethylene material, which is reliable in quality, dustproof, anti-fogging, and waterproof, and has good performance.

[0055] Obviously, the above embodiments of this utility model are merely examples for clearly illustrating the present utility model, and are not intended to limit the implementation of the present utility model. Those skilled in the art can make other variations or modifications based on the above description. It is neither necessary nor possible to exhaustively list all possible implementations here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of this utility model should be included within the protection scope of the claims of this utility model.

Claims

1. A cell culture tube, characterized in that, include: A tube (1), one end of which has an opening (11), and the interior of the tube (1) is used for culturing cells; The first cover (2) is detachably placed on the opening (11) to seal the pipe body (1). The second cover (3) is detachably installed on the pipe opening (11) or the first cover (2), and the second cover (3) is provided with a vent hole (31). A hydrophobic and breathable membrane (4) is disposed inside the second cover (3) and covers the vent holes (31).

2. The cell culture tube according to claim 1, characterized in that, The first cover (2) includes a sealing part (21) and a connecting part (22). The sealing part (21) is detachably covered on the pipe opening (11) to seal the pipe body (1). The connecting part (22) is connected to one axial end of the sealing part (21). The second cover (3) is detachably covered on the connecting part (22).

3. The cell culture tube according to claim 2, characterized in that, The outer periphery of the sealing part (21) is provided with a first anti-slip structure (211).

4. The cell culture tube according to claim 2, characterized in that, The outer periphery of the pipe opening (11) is provided with a first external thread (111), and the inner periphery of the sealing part (21) is provided with a first internal thread that can cooperate with the first external thread (111).

5. The cell culture tube according to claim 4, characterized in that, The outer periphery of the connecting part (22) is provided with a second external thread (221), and the inner periphery of the second cover (3) is provided with a second internal thread that can cooperate with the first external thread (111) or the second external thread (221).

6. The cell culture tube according to any one of claims 1-5, characterized in that, The second cover (3) has a second anti-slip structure (32) on its outer periphery.

7. The cell culture tube according to any one of claims 1-5, characterized in that, The ventilation holes (31) are provided in multiple ways, and the multiple ventilation holes (31) are distributed at intervals on the axial end face (33) of the second cover (3).

8. The cell culture tube according to claim 7, characterized in that, The vent holes (31) are circular and distributed in a circumferential array.

9. The cell culture tube according to any one of claims 1-5, characterized in that, The tube (1) is made of transparent material.

10. The cell culture tube according to claim 9, characterized in that, The tube body (1) is made of PETG material.

Images