Injector for an analysis device

Abstract

An injector (100) for an analysis device (10) for carrying out an analysis method is described, the injector (100) comprising: i) a housing (160); ii) a needle seat (140) disposed in the housing (160) adapted to receive a needle (110) for injection into an injection path (195); and iii) a movement device (145) coupled to the needle seat (140) and configured to move the needle seat (140) at least partially relative to the housing (160).

Description

FIELD OF THE INVENTION

[0001] The present invention relates to an injector for an analysis device for performing an analysis method, wherein the injector comprises: a housing, a needle seat arranged in the housing, which is configured to receive a needle for injection into an injection path, and a movement device coupled to the needle seat and configured to move the needle seat at least partially relative to the housing. Furthermore, the invention relates to an injector with a fastening device configured for releasably fastening an injector-associated device. Furthermore, the invention relates to the analysis device with the injector and a method for operating an injector. TECHNICAL BACKGROUND

[0002] Analysis devices are provided for the analysis of a sample, for example for carrying out a chromatographic separation of the sample.

[0003] In liquid separation in a chromatography system, for example, a mobile phase containing a fluid with the compounds to be separated is forced through a stationary phase (for example, a chromatographic column packing), thereby separating different compounds dissolved in or transported with the fluid, which can then be identified.

[0004] The mobile phase, which typically consists of one or more solvents, is pumped under high pressure through a chromatographic column containing a packing medium (also called the packing material or stationary phase). As the sample is carried through the column by the liquid stream, the different compounds, each of which has a different affinity for the packing medium, move through the column at different speeds. Those compounds that have a greater affinity for the stationary phase move more slowly through the column than those with a lower affinity. This difference in speed causes the compounds to become separated from each other as they pass through the column.The stationary phase is subjected to a mechanical force, generated in particular by a hydraulic pump, which generally pumps the mobile phase from an upstream port of the column to a downstream port. As a result of this flow, a relatively high pressure drop occurs across the column, depending on the physical properties of the stationary phase and the mobile phase.

[0005] The mobile phase containing the separated compounds leaves the column and passes through a detector, which registers and / or identifies the molecules, for example, through spectrophotometric measurements. A two-dimensional plot of the detector's measurements as a function of elution time or elution volume, called a chromatogram, can be created, and the compounds can be identified from the chromatogram. For each compound, the chromatogram displays a separate curve feature, also called a "peak."

[0006] In preparative chromatography systems, a liquid is usually provided as the mobile phase at a controlled flow rate (for example, in the range of 1 mL / min up to several 100 mL / min, for example, in preparative LC on an analytical scale in the range of 1 - 5 mL / min and on a preparative scale in the range of 4 - 200 mL / min) and at a pressure in the range of ten to one hundred bar, for example, 20 - 600 bar.

[0007] In high performance liquid chromatography (HPLC), a liquid mobile phase must usually be provided at a very controlled flow rate (for example in the range of microliters to milliliters per minute) and under high pressure (typically 20-100 MPa, 200-1000 bar and beyond up to currently 200 MPa, 2000 bar), at which the compressibility of the liquid becomes noticeable.

[0008] In analytical devices, particularly in liquid chromatography (especially HPLC), it can be important to reliably inject a sample into the analytical device. Typically, an injection / sample needle is used to aspirate a fluidic sample from a sample container, transport the sample to a sample injector of the analytical device, and inject the fluidic sample into a needle seat of the sample injector, thereby injecting the sample into an injection path of the analytical device.

[0009] Traditionally, the needle seat is stationary, i.e., fixed in the sample injection area, generally in a sampler (sampler). The needle is generally attached to a movable robotic arm that moves automatically within the sampler. The movable arm places the needle above the needle seat and then moves the needle downward so that the needle approaches an opening (for sample injection) of the sample injector. Other axis systems for positioning the needle also exist, e.g., a linear axis system (xyz) or a combination of a rotary axis (platter) and linear axes, as shown in US 9289768 B2.

[0010] Conventionally, the injection needle is either part of the robot or a separate assembly. Replacing a needle that is permanently connected to the robot carries the risk of damaging the robot; moreover, accessibility is limited. Replacing a needle that is part of an assembly requires manual removal (one of the user's hands is already occupied for this) and then disconnecting it from the system. This carries the risk of damaging the sample loop connected to the needle. Furthermore, the user only has one hand free for this task.

[0011] Furthermore, the needle / injection seat is conventionally an integral part of the injector and must be removed from its fixed position for maintenance, cleaning, or repair purposes. The injection seat is functionally located inside the injector, making access for maintenance, cleaning, or repair purposes difficult.

[0012] Furthermore, the conventional robot arm (with sample needle) does not remain in a defined position when de-energized (without force) and can move freely sideways. A sample plate (rotary plate) can also remain in an undefined (movable) position. This can significantly complicate access to the sample needle (from the front of the housing). SUMMARY OF THE INVENTION

[0013] There may be a need to operate an injector for an analysis device in an efficient and reliable manner. This object is achieved by the independent claims. Further embodiments are presented in the dependent claims.

[0014] According to one aspect, an injector (or sample injector, sample dispenser) is described for an analysis device (for carrying out an analysis method, e.g. HPLC), the injector comprising: i) a housing (e.g. a housing of an injector module or an overall housing in which further functionalities are arranged); ii) a needle seat (e.g. comprising an injection column), arranged in / on the housing, which is adapted to receive a needle (sample needle) for an injection (of a fluidic sample) into an injection path (e.g. arranged in the injection column; a fluid connection to a sample separation device); and iii) a movement device (e.g. designed as a drawer) (active or passive) which is coupled to the needle seat (in particular is fastened to the needle seat) and is configured to move the needle seat at least partially relative to the housing (in particular to move it at least partially out of the housing and / or to expose it).

[0015] According to one aspect, an injector (or sample injector, sample dispenser) is described for an analysis device (for carrying out an analysis method, e.g. HPLC), the injector comprising: i) an injector-associated device, in particular a handling device (e.g. a robot arm) for handling a needle, which is coupled and / or can be coupled to the handling device via a needle holder. ii) a fastening device (e.g. in / on a housing of the injector; e.g. designed as a locking device) arranged for (releasably) fastening the injector-associated device (e.g. one of the following: the needle, the needle holder, the handling device, the needle seat, the movement device).

[0016] According to one aspect, a method is described for operating an injector for an analytical device (for performing an analytical method), the method comprising: i) moving an injector-associated device (e.g., a handling device) (in a housing of the injector), ii) releasably fixing (fastening) the injector-associated device (to / in the housing); and in this case iii) blocking a function of the injector, in particular an injection function (e.g., locking the de-energized handling device).

[0017] In the context of the present document, the term “injector” may in particular refer to a device configured as an interface between a (fluidic) sample and an analysis region. In the present context, the term “injector” may refer to a sampler, sampler, or sampler chamber. An injector may, for example, comprise a handling device for handling the fluidic sample. Furthermore, an injector may comprise a needle seat coupled to an injection path into which the sample can be injected. An injector may also comprise further injector-associated devices, e.g., a handling device or a calibration device for calibrating the handling device, in particular with regard to the needle. In one example, the injector comprises an opening for receiving an approaching needle loaded with the sample.The needle can be fluidically coupled to the injector and introduce the sample through the sample injector and into the analysis region, particularly through an injection path. The analysis region can be part of an analysis device, for example, a separation column of a chromatography instrument. The sample injector can be part of the analysis device or a standalone device coupled to the analysis device.

[0018] In the context of the present document, the term "housing" (or injector-associated housing) can be understood in particular as a structure (cover, envelope, sheath) for components. In one embodiment, the injector can be designed as an injector module and the housing can spatially delimit the injector module. In one embodiment, the housing can spatially delimit several functionalities (including the entire analysis device) in addition to the injector. In this example, for example, the injector housing would be that part of the overall housing that is associated with the injector.

[0019] In the context of the present document, the term "needle seat" may refer in particular to a device configured to receive an approaching needle, in particular one loaded with a sample. The needle seat may be part of the injector described above and include the opening for the needle. Thus, the needle seat may be specifically configured to receive the needle (for example, by an elongated member such as an injection column) so that the sample loaded by the needle can be introduced directly into the needle seat, in particular into an injection path coupled to the needle seat.

[0020] In the context of the present document, the term "moving device" can refer in particular to a device suitable for moving a needle seat with respect to a housing. The moving device can be detachably coupled / fixed to the needle seat or rigidly connected to the needle seat. In one embodiment, the moving device moves the needle seat at least partially out of the housing. In another embodiment, the moving device moves the needle seat within the housing. In one embodiment, an injection column or an injection funnel of the needle seat can be coupled to the moving device. In a further embodiment, the injection path (e.g., as a channel, hose, conduit, capillary, fluid line, etc.) can also be coupled to the moving device.In a simple embodiment, the movement device is designed as a plate that can be moved like a drawer. In a more complex embodiment, the movement device can comprise a mechanism that tilts the needle seat so that it can, for example, be folded out or pulled out lengthwise. In one embodiment, the movement device can also be designed using a hinge so that the needle seat can be rotated / folded out of the housing (in particular at an edge). In one embodiment, the movement device can be an active device, e.g. driven by a drive (e.g. electric motor). In one embodiment, the movement device can be a passive device and, for example, be pulled out of the housing manually or by a robot (in particular a handling device).In a further embodiment, the movement device can be moved by means of the handling device. In a specific embodiment, the handling device can be temporarily attached to the movement device and / or the needle seat. In this case, the movement device and / or the needle seat can be used as a fastening device (in particular, a locking device).

[0021] In the context of the present document, the term "fastening device" can refer in particular to a device suitable for fastening an injector-associated device to the housing or within the housing. The fastening can in particular be a releasable fastening or temporary fixing. An injector-associated device can be connected to the injection function, for example a handling device and its components such as a needle holder, needle holder fastening and guide device (pusher). These devices can be fastened to and held on the fastening device, for example a needle holder is clicked in to be able to change a needle. The fastening device can be accommodated in a space-saving manner, for example can be folded out from a housing wall. In one embodiment, the fastening device has one or more fastening elements for actively holding the injector-associated device.In one embodiment, the fastening device is suitable for fastening solely due to its structure. In one embodiment, the fastening device can be referred to as a "needle / loop exchange station." For example, components of the handling device are temporarily fixed to the fastening device.

[0022] In a specific embodiment, the fastening device can be designed as a locking device configured to lock (fix / attach, in particular temporarily / removably) an injector-associated device, in particular the handling device. For example, the locking device can be used as a funnel / cup-shaped calibration device for temporarily holding the needle, or the handling device with the needle. If the locking device is coupled to a movable device (such as a turntable), the handling device and turntable can, for example, mutually lock (or interlock, mutually fix) each other via the locking device.

[0023] In the context of this document, the term “analysis device” can in particular refer to a device that is capable and configured to examine a fluidic sample, in particular to separate it, and further in particular to separate it into different fractions. For example, such sample separation can be carried out by means of chromatography or electrophoresis. Preferably, the analysis device can be a liquid chromatography sample separation device. The analysis device is in particular configured to carry out an analysis method or a (possibly planned or programmed) sequence of analysis methods or procedures. Furthermore, the term “analysis method” can also be used as a representative of a sequence, a program, an execution list of the analysis methods, procedures or instructions, including adaptation, preparation, equilibration (intermediate) steps and the like.

[0024] In this document, the term “fluidic sample” is understood to mean a medium, more particularly a liquid, which contains the material to be analyzed (for example, a biological sample), such as a protein solution, a pharmaceutical sample, etc.

[0025] In the context of this document, the term "mobile phase" refers in particular to a fluid, more particularly a liquid, that serves as a carrier medium for transporting the fluidic sample between a fluid drive and a sample separation device. However, the mobile phase can also be used in a fluid conveying device to influence the fluidic sample. For example, the mobile phase can be a solvent (e.g., organic and / or inorganic) or a solvent composition (e.g., water and ethanol).

[0026] In the context of the present document, the term “handling device” can be understood in particular as a device with which an operating medium, e.g. a fluid or a fluid receiving device, can be handled, that is to say e.g. at least one of transporting, receiving, dispensing, sucking up, injecting, storing, organizing, rotating, etc. Such a handling device can e.g. be a sample handling device which is set up to handle operating resources associated with a sample or with it, e.g. a fluidic sample, sample container, or sample container holding device.

[0027] In one embodiment, the resource handling device may be referred to as a “robot-assisted movement device,” in particular as an “external robot-assisted loading mechanism” (loading / unloading).

[0028] In a preferred embodiment, a handling device can be implemented as a robot arm, e.g., with or without a cantilever arm. Such a robot arm can also be designed, e.g., as a mobile robot head. In one embodiment, the robot arm can be maneuvered in at least two, preferably all three, spatial directions (within the sample space).

[0029] According to an exemplary embodiment, the invention can be based in particular on the idea that an injector for an analysis device can be operated efficiently and reliably if a movement device is coupled to the needle seat in such a way that the needle seat can be moved relative to the housing by means of the movement device (e.g., into an exposed position). Additionally or alternatively, a fastening device can be provided to / in which an injector-associated device (in particular a handling device) can be fastened or releasably fixed.

[0030] Conventionally (see above), activities such as maintenance, cleaning, repair, replacement, etc., can be quite challenging with regard to an injector of an analytical device. However, according to the invention, these disadvantages can be overcome in a simple, yet highly efficient and innovative way.

[0031] The described movement device can move the needle seat from an operating position to a convenient position, preferably exposed and / or outside the housing. This can be implemented, for example, in the form of a drawer, a pull-out mechanism, or a tilting / untilting mechanism. This allows the needle seat to be easily accessible to a user, allowing procedures such as maintenance, cleaning, repair, replacement, etc. to be performed efficiently and safely.

[0032] The described fastening device can temporarily secure / fix an injector-associated device such as a handling device, thus storing it safely and in a space-saving manner. The user then has both hands free, for example, to replace a needle on the fixed handling device.

[0033] Furthermore, the robot arm can be temporarily immobilized using a fastening device designed as a locking device. Otherwise, the robot arm would be free to move in space when de-energized, which could be detrimental to both safety and precision. This approach can be particularly advantageous if the locking device is coupled to a movable device, thus providing mutual locking. EXEMPLARY IMPLEMENTATION EXAMPLES

[0034] In one embodiment, the movement device is configured to perform at least one of the following movements relative to the housing: pushing, pulling, tilting, rotating, pivoting, or spinning. In one embodiment, the movement device is at least partially designed as a drawer. This can have the advantage that the movement device can be used and implemented in a very (design) flexible manner. Based on the desired application, the needle seat can be moved in different ways relative to the housing (in particular, into the housing, out of the housing, etc.). In one embodiment, the movement device is designed as a drawer-like element that can be pulled out of the housing (cf. Fig. 2B). In one embodiment, the movement device can be tiltable, so that the needle seat is tilted during movement. In one embodiment, the needle seat can be rotated out of the housing by means of the movement device. A locking or even ejection (e.g., via a spring mechanism) (and subsequent locking) can also be advantageous in some applications.

[0035] In one embodiment, moving the movement device relative to the housing moves the needle seat at least partially out of the housing. This can have the advantage that the needle seat is significantly easier to reach, thereby increasing practicality. In one embodiment, the needle seat is exposed to a user by the movement. In one embodiment, the needle seat and / or the movement device is / are arranged in a front region of the injector (with respect to a user). This can have the advantage that only a short distance has to be bridged by the movement device when moving out.

[0036] In one embodiment, the axis of the needle seat (in particular along the main extension direction (of the needle seat, e.g. of the injection column; e.g. vertically)) is changed when moved relative to the housing (e.g. when tilting) or remains the same (e.g. when pushing, pulling, rotating). The axis of the needle seat can be defined by its main extension direction. This is usually parallel to the injection path and mostly vertical. If this axis does not change, the needle seat is held in position and not tilted. This can have the advantage that the position of the injection path is essentially unchanged, thereby avoiding damage. However, tilting can, for example, save space. For example, the needle seat can be pushed lengthwise into the housing (towards / away from the user). In one example, the needle seat can be tilted (back) in the housing.

[0037] In one embodiment, the movement of the movement device relative to the housing comprises: an operating position, in particular in which an injection (into the injection path) is enabled. In one embodiment, the movement of the movement device relative to the housing comprises: an exposed position, in particular in which an injection (into the injection path) is impossible. An operating position can be, for example, a normal operating position (as with a stationary needle seat) in which the injection can be performed (cf. Fig. 2A). An exposed position can, for example, be a position that deviates from the operating position. For example, the needle seat can be moved by means of the movement device such that it is at least partially exposed to a user, in particular moved out of the housing. In a preferred embodiment, the injector (or the control device) can detect when such an exposed position exists and block an injection function accordingly. This can increase safety and reliability.

[0038] In one embodiment, the exposed position can be detected / detected by at least one of the following: electrical contact, light barrier or other optical detection, pressure contact switch, etc. In one embodiment, closing of the doors of the housing (or injector module) may no longer be possible (prevented) due to the protruding needle seat (exposed position) or the needle holder mounted on the housing (fastening device), while closed doors indicate the device's readiness for operation (e.g., through a closing contact). The exposed position can also be detected by the control of the handling device if an unexpected position is (or is not) reached (illustratively: the arm may move lower than expected or rest on the collecting funnel).

[0039] In one embodiment, the injector comprises: a fixing mechanism configured for (in particular releasably) fixing the movement device (in a specific position). In one embodiment, the movement device can be fixed, for example, in the operating position and / or in the exposed position. This can make operation efficient and safe because the desired position is reliably held. In one embodiment, the fixing mechanism can comprise at least one of the following: pushing out, pushing in, pulling out, pushing in, folding out, folding in, turning out, turning in, screwing in, screwing out, unscrewing, clicking in, clicking out, latching in, unlatching, magnetic. A variety of fixing mechanisms are conceivable. For example, the movement device can be configured as a drawer that is latched in the operating position and the exposed position.Such a temporary fixation can also be released again in a known manner.

[0040] In one embodiment, the injector is further configured to (e.g., by means of a control device): block a function of the injector, in particular an injection function, when the movement device is moved out of the operating position and / or is in the exposed position. This can have the advantage that the injector function is performed exclusively in the operating position provided for it. This can increase safety and reliability. Similarly, maintenance / cleaning / replacement of the needle seat can be made impossible in the operating position (while it is enabled in the exposed position).

[0041] In one embodiment, the injector further comprises a (sample) handling device (in particular a robot arm) for handling the needle, which is coupled and / or can be coupled to the handling device. In one embodiment, the handling device is configured to move the needle relative to the needle seat. In one embodiment, the movement of the needle relative to the needle seat takes place exclusively in the operating position. In this way, the needle can be handled efficiently.

[0042] In the present context, the term “handling device” can in particular refer to a device that is suitable for handling (in particular picking up, transporting, storing, injecting) a fluidic sample. Such a handling device can, for example, be used in an injector module and handle the sample between the sample vial and the injection path in a sample dispenser chamber. In one embodiment, the handling device has a mechanical movement functionality, for example, it is implemented as a robot arm (e.g. with a cantilever arm) or robot head (e.g. movable along a rail). A handling device can, for example, have a drive and be movable in two or three spatial directions. Coupling of the needle can, for example, be realized via a needle holder. The latter can be detachably coupled to the robot functionality via a needle holder attachment.

[0043] In one embodiment, the movement device and / or the needle seat is / are at least partially floating (movably) mounted. This can have the advantage of allowing the needle to be inserted precisely into the needle seat. If the needle seat is (slightly) movable, it can adapt to the position / orientation of the descending needle. In addition to or alternatively to the needle seat, the movement device (to which the needle seat is coupled) can be floating and enable compensation.

[0044] The term "floating" can in particular refer to a certain type of mounting in which the floating device (in particular a needle seat) is mounted in a movable manner (for example, on a needle seat mounting device). The term "floating" can, for example, refer to the fact that the needle seat is movable along at least one axis / direction (comprises one degree of freedom). In one example, the mounting device can be movable within a tolerance range, for example, planar (along the XY plane) and / or vertical (along the Z direction). The term "floating" can describe the opposite of a non-floating manner, for example, a fixed mounting or a stationary mounting.In one example, the floating is realized by a floating area between a needle seat and a needle seat mounting device, wherein the floating area comprises a non-locking material, for example, a lubricant, or a non-locking bearing, for example, a ball bearing. Additionally or alternatively, the mounting device may have a tolerance range / space that allows for a certain movement of the mounted needle seat.

[0045] In one embodiment, the needle seat has an injection column that at least partially encloses the injection path. This allows the injection path (e.g., a fluid line such as a capillary) to be protected. In one embodiment, the injection column is (detachably) coupled to the movement device. In one embodiment, the main extension direction of the injection column (vertical) and the movement device (horizontal) are oriented perpendicular to one another. This allows the movement device and needle seat to be coupled efficiently and robustly. In one embodiment, the needle seat (above the injection column) has a funnel-shaped structure that tapers from top to bottom. This structure can simplify / enable precise insertion of the needle into the needle seat.

[0046] In one embodiment, the injector (in particular the needle seat) has a washing device (in particular a shower device) for cleaning the needle. Such a washing device can be coupled, for example, to the injector funnel structure. Furthermore, such a washing device can serve as a fastening device, in particular a locking device, for temporarily holding / fixing the handling device.

[0047] In one embodiment, the fastening device is configured to fasten / fix the injector-associated device in such a way that a needle replacement, in particular (manually) by a user, is possible. This can have the advantage that maintenance, cleaning, or replacement can be carried out efficiently. The user is given flexibility and can have both hands free while, for example, the needle holder is fixed.

[0048] In one embodiment, the locking device (as an example of a fastening device) can prevent the handling device from moving freely in the sample space (e.g. when de-energized) (usually such handling devices are implemented without a braking function).

[0049] In one embodiment, the fastening device is designed to be movable, in particular between a rest position and a fastening position. This can have the advantage that the fastening device can be accommodated in a space-saving manner during normal operation. For example, the fastening device can be folded out from a housing wall if necessary. In one embodiment, moving the fastening device comprises at least one of the following: pushing out, pushing in, pulling out, pushing in, folding out, folding in, turning out, turning in, screwing in, screwing out, unscrewing, clicking in, clicking out, latching in, latching out.

[0050] In one embodiment, the injector is further configured to (by means of a control device): block a function of the injector, in particular an injection function, when the fastening device fastens the injector-associated device and / or is in the fastening position. This can increase safety and allow a user to safely maintain / clean / replace elements / components.

[0051] In one embodiment, the fastening device, in particular the locking device, is arranged on at least one of the following: a housing wall, a sample plate, a turntable, a calibration device, in particular a tapered calibration device (e.g., funnel- / cup- / truncated cone-shaped). This can have the advantage that existing structures of the injector or analysis device can be used directly. This can save costs and space; and the functionality of existing structures can be increased.

[0052] In one embodiment, the arm is first moved into the calibration device so that the pusher and calibration device / locking device touch each other, thus interlocking the plate and the arm in a mutually held position. The needle / holding device can then be withdrawn, for example, through an opening in the calibration device. The needle / holding device can then be inserted into the fastening device (here, separate from the locking device), mounted on the housing below the plate.

[0053] A locking device such as a calibration device (compare Fig. 4 and Fig. 5 below) can, for example, be used to calibrate the (position of) the sample needle and / or handling device. The calibration device can be a structure which, due to this structure, forces the needle into a specific position in order to enable calibration. This calibration structure can be used according to the invention to fix the needle or the injector-associated device (and via this the handling device) in this position. If, for example, the sample needle is moved into the funnel-shaped structure, it can no longer be moved out laterally; this can happen, for example, if the handling device is not supplied with power.

[0054] In one embodiment, the fastening device comprises a fastening element, in particular at least one of a holder, a gripper, a latching element, a click-in element, a screw element, or a magnet. In other words, the fastening device may comprise a specific fastening element to trigger the fastening. In one embodiment, the injection-associated device (e.g., the needle holder) may comprise a corresponding fastening element.

[0055] In one embodiment, the method for fixing / fastening comprises at least one of the following: fixing to a fastening device, fixing to a housing wall, fixing to / in a housing door, fixing to a sample plate or turntable, fixing to / in a washing device, fixing to / in a sample container, fixing to / in a calibration device, in particular a tapered calibration device, fixing to a recess in the calibration device. In one embodiment, the fixing comprises at least one of the following: mechanical, electrical, or magnetic.

[0056] In one embodiment, the method comprises maintaining / cleaning / replacing while the injector-associated device is (removably) fixed. In one embodiment, a needle and / or a needle holder may be replaced relative to the handling device while the handling device is removably fixed. In one embodiment, a needle may be replaced relative to the needle holder while the needle holder is removably fixed.

[0057] In an exemplary embodiment, the invention relates to a sample injector and specifically to the needle / seat assembly. Under operating conditions, the needle is inserted into the needle seat in a fluid-tight manner. However, when aspirating a sample from a vial, the needle can be removed from the seat.

[0058] In an exemplary embodiment, the needle is part of an assembly (needle holder) that is attached to a robotic arm (so that the robotic arm can move the needle as needed and also insert the needle into the needle seat). A special attachment feature or fastening device (e.g., on the housing of the injector) can now be provided that allows the needle holder to be attached to such a attachment feature when it is removed from the robotic arm. Once the needle holder is held by the attachment feature, certain maintenance tasks, such as replacing the needle, can be performed. Without such a attachment device, the needle holder must be held in one hand, making such maintenance tasks difficult. This can be extremely convenient (since the person now has two hands available for maintenance tasks).

[0059] One aspect of the invention can be seen in providing a fastening function on the housing of the injector, which enables the needle holder to be attached (when it is removed from the robot arm), e.g., for performing service activities on the needle holder. Furthermore, the injector can detect when the needle holder is attached to the fastening device, and the injector can then be blocked for normal operation, e.g., the injector is locked in a maintenance / service mode. A recess and / or a funnel-shaped calibration structure can also serve as the fastening device.

[0060] In an exemplary embodiment, the needle seat is arranged on a movable element (moving device) that can be pushed out relative to the injector housing, thus enabling, for example, easy cleaning, etc. The movement of the movable feature can be translation, rotation, pivoting, etc., to move the needle seat away from the injector for easier access. One aspect can be that the injector detects when the moving device is pushed out, and the injector is then blocked for normal operation, i.e., the injector is locked in a maintenance / service mode.

[0061] In one embodiment, the handling device is configured to move the movement device. In one example, the handling device can actively move the movement device (e.g., pushing it out / in). In another example, the handling device (e.g., when moving in the Z direction) can activate a mechanism that then moves the movement device (e.g., a folding mechanism for a passive movement device or activating a drive for an active movement device).

[0062] In one embodiment, the needle seat and / or the movement device can be used as a fastening device (in particular a locking device). In one example, the handling device (or the needle) can be locked in the needle seat or a washing device of the needle seat. In another example, the handling device can be (removably / temporarily) attached to the movement device or the needle seat, e.g., to securely move the movement device in the attached position.

[0063] According to one embodiment, the analysis device is designed as a sample separation device. According to one embodiment, the analysis device has a fluid drive for driving a mobile phase and a fluidic sample injected into the mobile phase. According to one embodiment, the analysis device has a sample separation device for separating the fluidic sample injected into the mobile phase. According to one embodiment, the analysis device is configured to analyze at least one physical, chemical and / or biological parameter of the fluidic sample. According to one embodiment, the analysis device is configured as a sample separation device for separating the fluidic sample.

[0064] In the context of the present application, the term "sample separation device" can be understood, in particular, as a device for analyzing a fluid sample, in particular into different fractions. For this purpose, components of the fluid sample can first be adsorbed on the sample separation device and then desorbed separately (in particular fractionally). For example, such a sample separation device can be designed as a chromatographic separation column.

[0065] According to one embodiment, the analysis device is a chromatography device, in particular a liquid chromatography device, a gas chromatography device, an SFC (supercritical fluid chromatography) device or an HPLC (high performance liquid chromatography) device.

[0066] According to one embodiment, the analysis device is configured as a microfluidic device. According to one embodiment, the analysis device is configured as a nanofluidic device.

[0067] According to one embodiment, the sample separation device is designed as a chromatographic separation device, in particular as a chromatography separation column.

[0068] According to one embodiment, the fluid drive is configured to drive the mobile phase and the fluidic sample under high pressure.

[0069] According to one embodiment, the fluid drive is configured to drive the mobile phase and the fluidic sample with a pressure of at least 500 bar, in particular of at least 1000 bar, further in particular of at least 1200 bar, further in particular of at least 1500 bar.

[0070] According to one embodiment, the analysis device comprises a detector for detecting the analyzed, in particular separated, fluidic sample.

[0071] According to one embodiment, the analysis device comprises a fractionator for fractionating separate fractions of the fluidic sample.

[0072] The analytical device can be a microfluidic instrument, a life science device, a liquid chromatography device, a gas chromatography device, an HPLC (high-performance liquid chromatography), a UHPLC system, or an SFC (supercritical fluid chromatography) device. However, many other applications are possible.

[0073] According to one embodiment, the sample separation device can be designed as a chromatographic separation device, in particular as a chromatography separation column. In a chromatographic separation, the chromatography separation column can be provided with an adsorption medium. The fluid sample can be retained on this adsorption medium and only subsequently detached fractionally in the presence of a specific solvent composition, thus achieving the separation of the sample into its fractions.

[0074] A pump system for conveying fluid can, for example, be configured to convey the fluid or mobile phase through the system at high pressure, for example, from a few hundred bar up to 1000 bar and more. The analysis device can have a sample injector for introducing the sample into the fluidic separation path. Such a sample injector can have a sample or injection needle that can be coupled to a needle seat in a corresponding fluid path, wherein the sample needle can be moved out of this needle seat to collect sample. After reinserting the sample needle into the needle seat, the sample can be located in a fluid path that can be switched into the separation path of the system, for example, by switching a valve. In another embodiment of the invention, a sample injector or sampler can be used with a sample needle that is operated without a needle seat.

[0075] The analysis device may include a fraction collector for collecting the separated components. Such a fraction collector can, for example, direct the various components of the separated sample into different liquid containers. The analyzed sample can also be fed to a waste container.

[0076] Preferably, the analysis device may include a detector for detecting the separated components. Such a detector may generate a signal that can be observed and / or recorded and is indicative of the presence and quantity of the sample components in the fluid flowing through the system. BRIEF DESCRIPTION OF THE DRAWINGS

[0077] Other objects and many of the attendant advantages of embodiments of the present disclosure will be readily appreciated and better understood by reference to the following more detailed description of embodiments taken in conjunction with the accompanying drawings. Features that are substantially or functionally the same or similar are designated by the same reference numerals. Fig. 1 shows an analysis device with a sample injector according to an exemplary embodiment. Fig. 2A shows an injector with a movement device in an operating position, according to an exemplary embodiment. Fig. 2B shows the injector of Fig. 2A with the movement device in an exposed position, according to an exemplary embodiment. Fig. 3A shows an injector with a fastening device, according to an exemplary embodiment. Fig. 3B shows the injector of Fig. 3A with an injector-associated device attached to the mounting device, according to an exemplary embodiment. The Fig. 4A to 4C show a temporary fastening of a handling device by means of a calibration device, according to an exemplary embodiment. Fig. 5 shows a detailed view of Fig. 4C, wherein the calibration device additionally has a recess for pushing an object through, according to an exemplary embodiment. DETAILED DESCRIPTION OF THE DRAWINGS

[0078] The drawings are schematic.

[0079] Fig. 1 shows an analysis device 10 with a sample injector 100 according to an exemplary embodiment. In Fig. 1 initially shows a general schematic of an analysis device 10. A fluid drive or solvent drive 20 (e.g., a pump) receives a solvent as the mobile phase from a solvent supply 25. The solvent drive 20 drives the mobile phase through a separation device 30 (e.g., a chromatographic column), which can be considered here as the analysis section of the device. Between the solvent drive 20 and the separation device 30, the injector 100, configured as a sample injector 40 (also called a sample introduction device, sample dispatcher, etc.), is provided to introduce or add portions of one or more sample liquids to the mobile phase stream at a mixing point 45 (often referred to as sample introduction). The separation device 30 is suitable for separating compounds of the sample fluid, e.g., a liquid.A detector 50 is provided for detecting the separated compounds of the sample fluid. A fractionation unit 60 may be provided for dispensing the separated compounds of the sample fluid. In one embodiment, at least parts of the sample injector 40 and the fractionation unit 60 may be combined, for example, by using common hardware that is employed by both the sample injector 40 and the fractionation unit 60.

[0080] The separation device 30 may comprise a stationary phase configured to separate compounds of the sample fluid. Alternatively, the separation device 30 may be based on another separation principle (e.g., field-flow fractionation).

[0081] The mobile phase may comprise only one solvent, but it may also be a mixture of a plurality of solvents (solvent supply 25). Such mixing may be a low-pressure mixture and take place upstream of the solvent drive 20, so that the solvent drive 20 already receives and delivers the mixed solvents as the mobile phase. Alternatively, the solvent drive 20 may comprise several individual pumping units, with several of these pumping units each receiving and pumping a different solvent or mixture, so that the mixing of the mobile phase (as received by the separation device 30) takes place under high pressure and downstream of the solvent drive 20 (or as part thereof). The composition (mixture) of the mobile phase can be kept constant over time, the so-called isocratic mode, or varied over time, the so-called gradient mode.

[0082] A control device 70, which may be a conventional PC or workstation, may be coupled (as indicated by the dashed arrows) to one or more of the devices in the analysis device 10 to receive information and / or control operation.

[0083] A fluidic sample is injected through a needle seat 140 into an injection path 195 of the analysis device 10. The needle seat 140 is connected (via the injection path 195) to the mixing point 45, where the sample is injected into a high-pressure path.

[0084] The fluidic sample is handled / transported by a handling device 190, here a sample handling device 190 (robot arm), which takes the sample from a sample container 130 (here a sample vial), transports the sample to the needle seat 140 and injects the sample into the needle seat 140.

[0085] The handling device 190 comprises a cantilever arm 178 connected at one end to a drive unit 128, wherein, for example, a first drive unit is provided for vertical movement (along Z) and a second drive unit for horizontal movement (along XY). At the other end, the cantilever arm 178 is coupled to a needle holder attachment 191, to which, in turn, a needle holder 192 is attached for holding the sample needle 110. The handling device 190 is configured to move the sample needle 110 into the sample container 130 so that the sample needle 110 picks up the sample and is then moved into the needle seat 140 for injecting the sample.

[0086] A pusher device or guide device (“pusher”) 115 surrounds the sample needle 110 to protect and improve sample pickup (e.g., pushes back the sample container 130 after the sample pickup or pushes / press it off so that it remains in the sample holder 130).

[0087] Fig. 2A shows an injector 100 with a movement device 145 in an operating position, according to an exemplary embodiment. The injector 100 has a housing 160 and a needle seat 140 arranged in the housing 160. The injector 100 can be designed as an injector module, and the housing 160 can spatially delimit the injector module. However, the housing 160 can also spatially delimit multiple functionalities (including the entire analysis device 10); in this case, for example, the injector housing 160 would be that part of the overall housing associated with the injector 100. The needle seat 140 is configured to receive a needle 110 for injection into an injection path 195.

[0088] In the example shown, the needle seat 140 has an injection column 141 (here oriented vertically), in which the injection path runs linearly as a channel (capillary). An injection funnel (collar) 142 is arranged above the injection column 141. The injection funnel 142 tapers from top to bottom, allowing the sample needle 110 to access the actual injection path 195 in a simplified manner. The injection column 141 serves as mechanical protection for the injection path 195 or the injection capillary.

[0089] The injector 100 has a handling device 190, here a robot arm, for handling the sample needle 110, wherein a cantilever arm 178 of the handling device 190 is coupled to the handling device 190 via the needle holder 192 and the needle holder fastening device 191. The handling device 190 is configured to move the needle 110 relative to the needle seat 140 (in particular exclusively in the operating position, see below). The needle holder fastening device 191 has a pusher device or guide device 115 (the "pusher"), which surrounds / encloses the sample needle 110 along its longitudinal extent. The pusher device 115 has an opening 116 (in a horizontally arranged plate) through which the sample needle 110 can be guided, e.g., for injecting / aspirating sample.

[0090] The injector 100 has a movement device 145 that is coupled to the needle seat 140 and is configured to move the needle seat 140 relative to the housing 160. In this example, the movement device 145 is designed as a drawer-like plate that can be pushed out of the housing 160 or pushed back in. The injection column 141 of the needle seat 140 is oriented perpendicularly (main extension direction in the vertical direction) to the plate (main extension in the planar plane) and is fastened thereto. Moving the movement device 145 correspondingly moves the needle seat 140 (and also the injection path 195). The movement device 145 has a handle 147 (here an opening) to manually perform the movement or withdrawal.

[0091] The viewing angle of this figure is essentially from the front of the housing front (shown schematically). Accordingly, the movement device 145 can be pulled out of the housing 160 towards a user. Fig. 2A, however, the movement device 145 is in a (normal) operating position.

[0092] The injector 100 further comprises a fastening device 150. The fastening device 150 is arranged / fastened here to the housing 160 of the injector 100 and is configured for releasably fastening an injector-associated device. Such a device can be, for example, one of the following: the needle 110, the needle holder 192, the needle holder fastening device 191, the handling device 190. The fastening device 150 can, for example, be configured to fix the needle holder fastening device 192 in such a way that a user can easily replace the needle 110.

[0093] In the example shown, the fastening device 150 is designed to be movable between a rest position 151 and a fastening position 152 (compare Fig. 2B below). Moving the fastening device 150 may, for example, comprise screwing out / in. The injector 100 may be configured such that a function of the injector (in particular, the injection function) 100 is blocked when the fastening device 150 fastens the injector-associated device and / or is in the fastening position.

[0094] Fig. 2B shows the injector of Fig. 2A with the movement device 145 in an exposed position, according to an exemplary embodiment. In comparison to Fig. 2A, the movement device 145 (with the coupled needle seat 140) has been moved relative to the housing 160; specifically, it has been pulled out of the housing 160. The needle seat 140 (in particular, the injection column 141 and injection funnel 142) are now easily accessible to a user, so that, for example, maintenance, cleaning, or replacement can be carried out.

[0095] Moving the movement device 145 relative to the housing 160 moves the needle seat 140 out of the housing 160 and exposes the needle seat 140 to a user, particularly because the needle seat 140 and the movement device 145 are arranged in a front region of the injector. The axis of the needle seat 140 along the main extension direction of the needle seat 140 or the injection column 141 (here vertical) is not changed during the movement relative to the housing 160 (displacement, not tilting). Fig. Figure 2B shows the exposed position where injection into the injection path 195 is impossible.

[0096] Furthermore, this example shows a washing station (in particular, a shower station) 146, where the sample needle 110 can be cleaned. In this example, the washing station 146 is attached directly to the injection funnel 142 and can be moved together with the needle seat 140.

[0097] Fig. 2B also shows that an injector-associated device (here the needle holder 192) is temporarily fixed to the fastening device 150, e.g., clicked in. This allows, for example, a needle replacement to be easily performed because a user "has his hands free."

[0098] Fig. 3A shows an injector 100 with a fastening device 150, according to an exemplary embodiment. Fig. 3A is Fig. 2A and schematically shows that the needle holder 192 (for directly attaching the sample needle 110) can be transferred from the needle holder attachment 191 on the handling device 190 to the attachment device 150. Furthermore, a sample loop 118 is shown, which is coupled to the sample needle 110 and in which the sample can be stored.

[0099] Fig. 3B shows the injector 100 of Fig. 3A with an injector-associated device or the needle holder 192 attached to the attachment device 150, according to an exemplary embodiment. The needle holder 192 is now no longer coupled to the needle holder attachment 191 of the handling device 190 (this is free), but is temporarily fixed (together with the sample needle 110 and the sample loop 118) to the attachment device 150. This fixing enables efficient and safe replacement, e.g., of the sample needle 110 and / or sample loop 118. The needle holder 192 (e.g., with a new needle 110) can then be recoupled to the handling device 190.

[0100] The Fig. 4A to 4C show a temporary fastening of a handling device 190 by means of a locking device (as an example of a fastening device, here designed as a calibration device 120, according to an exemplary embodiment).

[0101] Fig. 4A: The handling device 190 (as described above, e.g., a cantilever arm 178 with needle holder attachment 191, needle holder 191, and sample needle 110) is moved downward. The locking device / calibration feature 120 ("calibration feature") is funnel- / conical-shaped and tapers from top to bottom.

[0102] Fig. 4B: The tapered shape of the calibration device 120 guides the descending handling device 190 (in particular, sample needle 110 and guide device 115) toward the center, thereby enabling efficient and safe insertion of the handling device 190. It can be seen that the guide device 115 was guided toward the center along the tapered side walls of the calibration device 120. This is possible, for example, because the handling device 190 is held in the plane without force / current, and its direction of movement is determined by the geometry of the calibration device 120.

[0103] Fig. 4C: The handling device 190 is now releasably fixed in the locking device / calibration device 120 as a fastening device 150. In this position, the handling device is held in place so that, for example, the sample needle 110 can be replaced. The calibration device 120 can be used, for example, to calibrate the sample needle 110 and / or control the handling device 190, while simultaneously serving as a fastening device 150 or locking device. The needle holder 192 (“needle and loop assembly”) can, for example, be released and pulled out in an extension direction.

[0104] Fig. 5 shows a detailed view of the principle of Fig.4C, wherein the calibration device 120 additionally has a recess 125, according to an exemplary embodiment. The plate of the guide device 115 practically rests on the side wall of the calibration device 120, and the needle can extend through the opening 116 into the calibration device 120. To change the needle, the recess 125 in the side wall of the calibration device 120 can be used to pull out the lower-lying needle 110.

[0105] In other words, the robot arm 190 moves with the pusher 115 into a calibration device 120 of a sample turntable. The pusher 115 catches in the calibration device 120 and is held there after the motor 128 is switched off. This keeps the handling device 190 and the turntable mutually in position. Reference symbol 10 Analysis device 20 Fluid drive, fluid drive device 25 Feeding device 27 degassers 30 Sample separation device 40 Sample injector, sample dispenser 45 mixing point 50 detector 60 fractionators 70 Control device 100 injectors 110 needle 115 Guide device 116 Opening of the guide device 118 Sample loop 120 locking device, calibration device 125 recess 128 drive 130 sample containers 140 needle seat 141 Injection column 142 injection funnels 145 Moving device, drawer 146 Washing station, shower 147 handle 150 fastening device 151 Folded position 152 Unfolded position, fastening element 160 housings 178 boom arm 190 Handling device, robot arm 191 Needle holder attachment 192 needle holders 195 Injection pathway QUOTES CONTAINED IN THE DESCRIPTION

[0000] This list of documents submitted by the applicant was generated automatically and is included solely for the convenience of the reader. This list is not part of the German patent or utility model application. The DPMA assumes no liability for any errors or omissions. Cited patent literature

[0000] US 9289768 B2

[0009]

Claims

[1] An injector (100) for an analysis device (10) for performing an analysis method, the injector (100) comprising: a housing (160); a needle seat (140) disposed in the housing (160) adapted to receive a needle (110) for injection into an injection path (195); and a movement device (145) coupled to the needle seat (140) and configured to move the needle seat (140) at least partially relative to the housing (160). [2] The injector (100) according to claim 1, wherein the movement device (145) is configured to perform at least one of the following movements relative to the housing (160): pushing, pulling, tilting, rotating, pivoting, spinning; and / or wherein the movement device (145) is at least partially designed as a drawer. [3] The injector (100) according to any one of the preceding claims, comprising at least one of the following features: wherein moving the movement device (145) relative to the housing (160) moves the needle seat (140) at least partially out of the housing (160), in particular exposing the needle seat (140) to a user; wherein the needle seat (140) and / or the movement device (145) is / are arranged in a front region of the injector (100); wherein the axis of the needle seat (140), in particular along the main extension direction of the needle seat (140), is changed or remains the same during the movement relative to the housing (160). [4] The injector (100) according to any one of the preceding claims, wherein moving the movement device (145) relative to the housing (160) comprises: an operating position, in particular in which an injection into the injection path (195) is possible, and an exposed position, in particular in which an injection into the injection path (195) is impossible. [5] The injector (100) according to any one of the preceding claims, further comprising: a fixing mechanism, configured for, in particular releasably, fixing the movement device (145), in particular by means of at least one of the following: pushing out, pushing in, pulling out, pushing in, folding out, folding in, turning out, turning in, screwing in, screwing out, clicking in, clicking out, latching in, latching out. [6] The injector (100) according to one of the preceding claims, further configured to, in particular by means of a control device (70): Blocking a function of the injector (100), in particular an injection function, when the movement device (145) is moved out of the operating position and / or is in the exposed position. [7] The injector (100) according to any one of the preceding claims, further comprising: a handling device (190), in particular a robot arm, for handling the needle (110), which is coupled and / or can be coupled to the handling device (190), wherein the handling device (190) is configured to move the needle (110) relative to the needle seat (140), in particular exclusively in the operating position. [8] The injector (100) according to any one of the preceding claims, wherein the movement device (145) and / or the needle seat (140) is / are at least partially floatingly mounted. [9] The injector (100) according to any one of the preceding claims, wherein the needle seat (140) has an injection column (141) which at least partially encloses the injection path (195), and wherein the injection column (141) is detachably coupled to the movement device (145), in particular wherein the main extension directions of the injection column (141) and the movement device (145) are oriented perpendicular to each other. [10] An injector (100) for an analysis device (10) for performing an analysis method, the injector (100) comprising: an injector-associated device, in particular a handling device (190) for handling a needle (110), which is coupled and / or can be coupled to the handling device (190) via a needle holder (192); and a fastening device (150), in particular in / on a housing (160) of the injector (100), configured for releasably fastening the injector-associated device, in particular at least one of the following: the needle (110), the needle holder (192), the handling device (190), a needle seat (140), a movement device (145). [11] The injector (100) according to claim 10, wherein the fastening device (150) is configured to fix the injector-associated device in such a way that an exchange of the needle (110), in particular by a user, is possible. [12] The injector (100) according to claim 10 or 11, wherein the fastening device (150) is arranged to be movable, in particular between a rest position (151) and a fastening position (152); and / or wherein moving the fastening device (150) comprises at least one of the following: pushing out, pushing in, pulling out, pushing in, folding out, folding in, turning out, turning in, screwing in, screwing out, clicking in, clicking out, engaging, disengaging. [13] The injector (100) according to any one of claims 10 to 12, further configured to: Blocking a function of the injector (100), in particular an injection function, when the fastening device (150), in particular designed as a locking device (120), fastens the injector-associated device and / or is in the fastening position (152). [14] The injector (100) according to one of claims 10 to 13, wherein the fastening device (150), in particular locking device (120), is arranged on at least one of the following: a housing wall, a sample plate, in particular a turntable, a calibration device (120), in particular a tapered calibration device. [15] The injector (100) according to one of claims 10 to 14, wherein the fastening device (150) comprises a fastening element (152), in particular at least one of a holder, a gripper, a latching element, a click-in element, a screw element, a magnet. [16] An analysis device (10) for performing an analysis method, wherein the analysis device (10) comprises an injector (100) according to one of the preceding claims. [17] The analysis device (10) according to claim 16, comprising at least one of the following features: the analysis device (10) is designed as a sample separation device; the analysis device (10) has a fluid drive (20) for driving a mobile phase and a fluidic sample injected into the mobile phase; the analysis device (10) has a sample separation device (30) for separating the fluidic sample injected into the mobile phase; the analysis device (10) is configured to analyze at least one physical, chemical and / or biological parameter of the fluidic sample; the analysis device (10) is configured as a sample separation device for separating the fluidic sample; the analysis device (10) is a chromatography device, in particular a liquid chromatography device, a gas chromatography device, an SFC (supercritical fluid chromatography) device or an HPLC (high performance liquid chromatography) device; the analysis device (10) is configured as a microfluidic device; the analysis device (10) is configured as a nanofluidic device; the sample separation device (30) is designed as a chromatographic separation device, in particular as a chromatography separation column; the fluid drive (20) is configured to drive the mobile phase and the fluidic sample under high pressure; the fluid drive (20) is configured to drive the mobile phase and the fluidic sample with a pressure of at least 500 bar, in particular of at least 1000 bar, further in particular of at least 1200 bar; the analysis device (10) has a detector (50) for detecting the analyzed, in particular separated, fluidic sample; the analysis device (10) comprises a fractionator (60) for fractionating separate fractions of the fluidic sample, in particular wherein the device (100) is at least partially implemented in the fractionator (60). [18] A method for operating an injector (100) for an analysis device (10) for performing an analysis method, the method comprising: Moving a handling device (190) in a housing (160) of the injector (100); releasably fixing the handling device (190) in / on the housing (160); and Blocking a function of the injector (100), in particular an injection function. [19] The method according to claim 18, wherein the fixing comprises at least one of the following: Fixing to a fastening device (150), fixing to a housing wall, fixing to / in a door of the housing (160), fixing to a sample plate, fixing to / in a washing device, fixing to / in a sample container (131), fixing to / in a calibration device (120), in particular a tapered calibration device, fixing to a recess (125) of the calibration device (120); and / or wherein the fixing comprises at least one of the following: mechanical, electrical, magnetic. [20] The method according to claim 18 or 19, further comprising: Exchanging a needle (110) and / or a needle holder (192) with respect to the handling device (190) while the handling device (190) is releasably fixed.

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