Electrophoresis device

Abstract

Electrophoresis apparatus comprising: a capillary (101) or a capillary arrangement (201) as an assembled set of several capillaries (101), a receiving optical system (202) that irradiates a sample in the capillary (101) or in the capillaries (101) with light and detects the fluorescence of the sample, a high-voltage source (102) that applies a high voltage to the capillary (101) or the capillaries (101), a constant-temperature oven (103) that maintains the capillary (101) or the capillaries (101) at a constant temperature, and a transport machine (203) that transports several containers including a sample container (210) and an electrophoresis medium container (105), wherein the capillary (101) or the capillaries (101) have a sealing element (215).which maintains a container body (214) filled with an electrophoresis medium in a sealed state and which can be penetrated by the capillary (101) or capillaries (101), wherein the sealing element (215) is made of elastically deformable rubber and maintains the sealed state of the container body (214) by elastic deformation, even when the capillary (101) or capillaries (101) penetrate the sealing element (215); wherein the sealing element (215) comprises a recessed section (301) into which the capillary (101) or capillaries (101) is / are inserted, a tapered section (302) from which an external force normal to an insertion section of the capillary (101) or capillaries (101) is exerted, and an O-ring (304) with which an intermediate space with the container main body (214) is filled, has; wherein,when the capillary (101) or capillaries (101) penetrate the sealing element (215), the electrophoresis medium in the main body of the container (214) is compressed by the amount of the volume of the capillary (101) or capillaries (101) introduced into the main body of the container (214) and is forced into the interior of the capillary (101) by the pressure thus generated by the increase in pressure in the main body of the container (214); and wherein the volume of the capillary (101) or capillaries (101) introduced into the main body of the container (214) is greater than the internal volume of the capillary (101) or capillaries (101).

Description

Technical field

[0001] The present invention relates to an electrophoresis medium container and an electrophoresis apparatus. The present invention relates, for example, to an electrophoresis medium container suitable for a capillary electrophoresis apparatus which separates and analyzes a sample such as DNA or a protein by electrophoresis. Technical background

[0002] In recent years, a capillary electrophoresis device has been widely used as the electrophoresis device, in which a capillary is filled with an electrophoresis medium in the form of a polymer gel or a polymer solution.

[0003] For example, a capillary electrophoresis device disclosed in PTL 1 was conventionally used. Compared to a flat-plate electrophoresis device, the capillary electrophoresis device offers high heat dissipation and allows for a higher voltage to be applied to the sample, resulting in the advantage of high-speed electrophoresis. Furthermore, it offers numerous advantages, including the ability to track samples, automatically fill the electrophoresis medium, and automatically inject the sample. Therefore, the capillary electrophoresis device can be used for various types of separations and analytical measurements, including nucleic acid and protein analysis.

[0004] Fig. Figure 1 shows an overview of a state-of-the-art capillary electrophoresis device. The capillary electrophoresis device is designed to include a capillary 101 , a high-voltage source 102 , which applies a high voltage to both ends of the capillary 101 installs a constant temperature oven 103 , which determines the temperature of the capillary 101 regulates, and an electrophoresis medium filling unit 104 , which the capillary 101 The capillary electrophoresis device, although not shown, may also include an irradiation system with a laser beam source or the like, a receiving optical system that detects fluorescence, a transport machine that moves a container holding a sample, or the like.

[0005] The anode side of the capillary 101is connected to a flow path of the electrophoresis medium filling unit 104 connected. The flow path in the electrophoresis medium filling unit 104 It branches into two flow paths. One flow path is connected to an electrophoresis medium container. 105 connected, and the other flow path is connected to a buffer solution container A 106 tied together.

[0006] In the capillary electrophoresis device, an electrophoresis medium with a viscosity more than 100 times that of water must be introduced into the capillary. 101 with an inner diameter of only about 50 μm. Therefore, for the electrophoresis medium filling unit 104 A mechanism is used that can exert a pressure of several MPa on one end of the flow path for the electrophoresis medium. One type of such mechanism is, for example, a piston pump. 107 . At the in Fig. In the case shown in 1, the piston pump107 The flow is driven in a direction perpendicular to the plane of the paper. In this way, the volume within the flow path is changed, generating a pressure required for filling with the electrophoresis medium.

[0007] During the analysis of a sample, a high voltage is applied to both ends (to the buffer solution container A). 106 and a buffer solution container B 109 ) of the capillary 101 connected flow path, and electrophoresis of a sample in the form of DNA with a fluorescent marker in the electrophoresis medium of the capillary is performed. 101 The electrophoresis rate depends on the molecule size, and the sample is passed through the detector in this way. 108 detected.

[0008] It should be noted that in the capillary electrophoresis device the electrophoresis medium container 105 and the capillary 101They need to be replaced. However, during the replacement, part of the flow path is exposed to air, which can cause air to be mixed into the flow path.

[0009] During electrophoresis, a high voltage of several to several tens of kV is applied to both ends of the flow path. Consequently, if bubbles are present in the flow path, there is a possibility that the bubbles will electrically interrupt the flow path. When the flow path is interrupted, a high voltage difference is generated at the interruption point, leading to a discharge. Depending on the magnitude of the discharge, there is a possibility that the capillary electrophoresis device could be damaged.

[0010] Therefore, there is a need to remove bubbles from the interior of the flow path before starting electrophoresis.

[0011] For example, in a case where bubbles are present in the flow path of the electrophoresis medium filling unit 104 are present, the connecting flow path between the electrophoresis medium filling unit 104 and the capillary 101 blocked, whereby in this state the electrophoresis medium flows through a branch path in the unit to the buffer solution container A 106 Bubbles flow back out of a flow path zone of the electrophoresis medium filling unit. 104 removed. The presence of bubbles in the flow path in the electrophoresis medium filling unit. 104 is checked by a user through viewing.

[0012] In comparison, the capillary 101 in a case where bubbles are present in the flow path of the capillary 101 are present, filled with an amount of electrophoresis medium that is twice the internal volume of the capillary. 101 . The capillary is involved.101 It is thin and has an inner diameter of approximately 50 μm. Accordingly, the bubbles flow within the capillary. 101 together with the electrophoresis medium and are drawn from the end on the other side of the capillary. 101 expelled. In other words, bubbles can escape from inside the capillary. 101 be removed.

[0013] For example, PTL 2 discloses a structure in which it is not necessary to visually inspect the electrophoresis medium filling unit for bubbles, thus reducing the operational complexity of the electrophoresis device. Specifically, the electrophoresis medium filling unit is mounted in both attachable and detachable modes. Only when the electrophoresis medium is being filled is the unit connected to a capillary. During electrophoresis, both ends of the capillary are directly immersed in a buffer solution, allowing the electrophoresis medium filling unit to be removed from the flow path during electrophoresis and eliminating the need for prior bubble inspection. List of citations from patent literature

[0014] PTL 1: Japanese Patent 2776208 PTL 2: JP-A-2012-2585 Summary of the invention: Technical problem

[0015] As a result of intensive investigations, the inventors of the present application found the following problems. In the capillary electrophoresis device disclosed in PTL 2, as described above, it is difficult to maintain tight contact (a seal) between the capillary and the electrophoresis medium container because the electrophoresis medium container moves during the filling of the capillary with the electrophoresis medium. This constitutes the first problem. Furthermore, because the electrophoresis medium container has a complex design, it is difficult to manufacture and to contain the electrophoresis medium. This constitutes the second problem. Finally, a significant amount of the electrophoresis medium is not used to fill the capillary (dead volume), which constitutes the third problem.

[0016] The present invention therefore solves the first, second and third problems and aims to provide an electrophoresis medium container and an electrophoresis device which accomplish the following three tasks.

[0017] The first task is to maintain a static state of the electrophoresis medium container while filling a capillary with electrophoresis medium, so that the capillary and the electrophoresis medium container can be easily sealed.

[0018] The second task is to simplify the shape of the electrophoresis medium container so that the electrophoresis medium container can be easily manufactured and the electrophoresis medium can be easily injected.

[0019] The third task, without limitation, is to enable the amount of electrophoresis medium enclosed in the electrophoresis medium container to approximate the amount of electrophoresis medium with which the capillary is filled, so that the dead volume can be minimized.

[0020] The foregoing and other tasks and novel features of the present invention will become clearer in the description of the present patent specification and with reference to the accompanying drawing. Solution to the problem

[0021] The following is a brief overview of representative embodiments of the invention disclosed in the present application.

[0022] In other words, the representative electrophoresis medium container has a sealing element that keeps the main body of the container, filled with electrophoresis medium, in a sealed state and that can be penetrated by the capillary. The electrophoresis medium is introduced into the interior of the capillary by the pressure generated when the capillary penetrates the sealing element, which seals the main body of the container filled with the electrophoresis medium.

[0023] It is preferred that, in the electrophoresis medium vessel, the pressure generated when the capillary penetrates the sealing element is a pressure produced by increasing the pressure in the vessel body by compressing the electrophoresis medium by the volume of the capillary introduced into the vessel body. It is even more preferred that the volume of the capillary introduced into the vessel body is greater than the internal volume of the capillary.

[0024] In the case of the electrophoresis medium vessel, it is even more preferred if the sealing element is made of a material that is likely to deform elastically and maintains the sealed state of the vessel body by elastic deformation, even when the capillary penetrates the element. It is even more preferred if the sealing element is made of rubber or a resin that is likely to deform elastically.

[0025] Furthermore, the invention can be applied to an electrophoresis device in which the electrophoresis medium container described above is used. Advantageous effects of the invention

[0026] The following briefly describes the effects obtained through representative embodiments of the invention disclosed in the present application.

[0027] The first effect is that it is possible to maintain a static state of the electrophoresis medium container while filling the capillary with the electrophoresis medium, and to easily seal the capillary and the electrophoresis medium container.

[0028] The second effect is that the shape of the electrophoresis medium container is simplified, making it easy to manufacture the electrophoresis medium container and to easily enclose the electrophoresis medium.

[0029] The third effect is that the amount of electrophoresis medium enclosed in the electrophoresis medium container can be brought close to the amount of electrophoresis medium filling the capillary without restriction, thereby minimizing the dead volume. Brief description of the drawing

[0030] They show:

[0031] Fig. 1. An overview of a capillary electrophoresis device according to the state of the art,

[0032] Fig. 2 an overview of an electrophoresis device according to embodiment 1 of the present invention,

[0033] Fig. 3 a disassembled configuration of an electrophoresis medium container according to embodiment 1 of the present invention,

[0034] Fig. 4 a composite state of the electrophoresis medium container according to embodiment 1 of the present invention,

[0035] Fig. 5 a state when using the electrophoresis medium container according to embodiment 1 of the present invention,

[0036] Fig. 6 a structure of a partition wall according to embodiment 1 of the present invention,

[0037] Fig. 7 a structure of the electrophoresis medium container according to embodiment 1 of the present invention,

[0038] Fig. 8 a receiving section of the electrophoresis medium container according to embodiment 1 of the present invention,

[0039] Fig. 9 the installation of the electrophoresis medium container according to embodiment 1 of the present invention,

[0040] Fig. 10 an installation state of the electrophoresis medium container according to embodiment 1 of the present invention,

[0041] Fig. 11 a filling state with an electrophoresis medium according to embodiment 1 of the present invention,

[0042] Fig. 12 an electrophoresis medium container according to embodiment 2 of the present invention,

[0043] Fig. 13 a configuration of an electrophoresis medium container according to embodiment 3 of the present invention,

[0044] Fig. 14 a configuration of an electrophoresis medium container according to embodiment 4 of the present invention,

[0045] Fig. 15 a configuration of an electrophoresis medium container according to embodiment 5 of the present invention,

[0046] Fig. 16 an overview of an electrophoresis device according to embodiment 5 of the present invention,

[0047] Fig. 17 an installation procedure of the electrophoresis medium container according to embodiment 5 of the present invention,

[0048] Fig. 18 a filling state with an electrophoresis medium according to embodiment 5 of the present invention,

[0049] Fig. 19 an installation procedure of an electrophoresis medium container according to embodiment 7 of the present invention,

[0050] Fig. 20 a disassembled configuration of an electrophoresis medium container according to embodiment 8 of the present invention,

[0051] Fig. 21 the configuration of the electrophoresis medium container according to embodiment 8 of the present invention,

[0052] Fig. 22 a state immediately after the introduction of a capillary cathode end according to embodiment 8 of the present invention,

[0053] Fig. 23 the introduction state of the capillary cathode end according to embodiment 8 of the present invention,

[0054] Fig. 24 a capillary arrangement according to embodiment 9 of the present invention,

[0055] Fig. 25 a capillary arrangement according to embodiment 10 of the present invention,

[0056] Fig. 26 an overview of an electrophoresis device according to embodiment 10 of the present invention,

[0057] Fig. 27 a filling state with an electrophoresis medium according to embodiment 11 of the present invention and

[0058] Fig. 28 an overview of an electrophoresis device according to embodiment 12 of the present invention. Description of embodiments

[0059] The following embodiments are subdivided into several sections or several embodiments as needed and for practical reasons, the embodiments relating to one another except where a specific description is provided, and in this relation one embodiment is a part, an entire modification example, a detailed description, or a supplementary description of the other embodiment. Additionally, in the following embodiments, where a number (including the number, value, quantity, range, or the like) of components is described, there is no limitation to a specific number, and a number may be greater, less than, or equal to the specified number, except where a specific description is provided and where there is a limitation to the specific number that is made clear by the principle.

[0060] Furthermore, it is unnecessary to note that in the following embodiment, components (including a component step or the like) are not required, except in a case where a specific description is provided or in a case where the components are deemed necessary, as is made clear by the principle. Similarly, according to the following embodiment, when a shape, positional relationship, or the like of components or the like is described, the shape or the like includes substantially similar shapes, except in a case where a specific description thereof is provided or in a case where the shape or the like is deemed incorrect, as is made clear by the principle. The same applies to the values ​​and ranges described above. [Overview of an embodiment]

[0061] First, an overview of one embodiment is described. In this overview of the present embodiment, reference numerals in parentheses are attached to components that correspond to the embodiment, as an example.

[0062] In other words, the electrophoresis medium container used as an example (electrophoresis medium container) 105 ) according to the present embodiment, a sealing element (a partition) 215 ) which has a main container body (container) filled with an electrophoresis medium 214 ) in a sealed state and which can be penetrated by the capillary. The electrophoresis medium is introduced into the interior of the capillary as a result of the pressure generated when the capillary penetrates the sealing element, which seals the main body of the container filled with the electrophoresis medium.

[0063] It is preferred that the pressure generated in the electrophoresis medium vessel when the capillary penetrates the sealing element is produced by increasing the pressure in the vessel body, compressing the electrophoresis medium by an amount equal to the volume of the capillary inserted into the vessel body. It is even more preferred that the volume of the capillary inserted into the vessel body is larger than the internal volume of the capillary.

[0064] It is even more preferred that the sealing element of the electrophoresis medium container is made of a material that, even when the capillary penetrates the element, is likely to deform elastically and maintain the sealing condition of the container body through elastic deformation. It is even more preferred that the sealing element is made of rubber or a resin that is likely to deform elastically.

[0065] Furthermore, the present invention is also applied to an electrophoresis device which uses the electrophoresis medium container.

[0066] The respective embodiments are described in detail below, based on the drawing and the overview of the embodiment described above. Furthermore, the same reference numerals are assigned to elements in all drawing sections used to describe the respective embodiments, and a repeated description of these elements is omitted. Additionally, the description of identical or similar sections is generally not repeated in the respective embodiments.

[0067] Additionally, in some drawing elements used in the respective embodiments, hatching in a section plane is omitted, even in sectional views, so that the drawing can be easily viewed. Hatching is applied even in a top view to ensure the drawing is easy to understand. [Version 1]

[0068] The electrophoresis device according to the present embodiment is described with reference to the Fig. 2 to Fig. 11. Furthermore, details of a configuration of the device or an operating process are provided in the following description only as an example to illustrate the invention and do not limit the scope of protection of the invention. Additionally, other embodiments can be realized not only by combining the respective embodiments but also by making substitutions between the respective embodiments and known technologies. <Overview of an electrophoresis device>

[0069] Fig. Figure 2 shows an overview of the electrophoresis device according to the present embodiment. A configuration of the electrophoresis device is described below with reference to... Fig. 2 described.

[0070] A capillary electrophoresis device according to the present embodiment comprises a capillary 101 or a capillary arrangement 201as an assembled set of several capillaries 101 , a receiving optical system 202 , which is a sample in the capillary 101 irradiated with light and fluorescence detected from the sample, a high-voltage source 102 to apply a high voltage to the capillary 101 , a constant temperature oven 103 to hold the capillary 101 at a constant temperature and an autosampler 203 as a transport machine for transporting a bowl 208 , on which several containers are mounted, containing a sample, an electrophoresis medium or the like.

[0071] One end of the capillary arrangement 201 is a capillary head 204 , which comes from the capillaries 101 It is formed, which are bundled and bonded. A detector 108 is provided at a section where the capillaries 101are bundled. The other end of the capillary arrangement 201 is in a charging head 205 held. The charging head 205 is at the constant temperature oven 103 attached.

[0072] A tubular cathode 206 is in the charging head 205 provided. The capillary 101 is through the cathode 206 from the lower end (hereinafter referred to as the capillary cathode end) 207 (designated) the cathode 206 before.

[0073] The capillary arrangement 201 is achieved by inserting the capillary cathode end 207 The electrophoresis medium reservoir is filled with the electrophoresis medium. As described below, the position at which the capillary cathode end is placed is determined. 207 is introduced, by using a partition that does not allow leakage even if the partition is penetrated, and is kept in a sealed state.

[0074] On the bowl208 The buffer solution container B will be 109 , into which the capillary cathode end 207 is immersed in a pure water container 209 , containing pure water for cleaning the capillary, an electrophoresis medium container 105 , containing the electrophoresis medium, and a sample container 210 , in which the sample is contained, is assembled. Furthermore, the capillary head is 204 at the capillary anode end into the buffer solution container A 106 immersed.

[0075] The autosampler 203 is designed to have two timing belts 211 exhibits which in Fig. 2 correspond to a right-left direction (horizontal direction: X) or an up-down direction (vertical direction: Y). A rotation of the two control belts 211 It allows the shell 208to transport in right-left and up-down directions. Transport in two axial directions allows the respective containers attached to the tray to 208 are mounted, positioned at a location corresponding to the capillary cathode end 207 opposite. Furthermore, the timing belts 211 by the rotation of a motor 213 driven by a pulley 212 is connected. <Struktur des Elektrophoresemediumbehälters>

[0076] A configuration of the electrophoresis medium container 205 , which is used in the capillary electrophoresis device, is referred to in relation to the Fig. 3, Fig. 4 and Fig. 5 described. Fig. Figure 3 is a view (perspective single-part view) of a disassembled configuration of the electrophoresis medium container. 105 . Fig. Figure 4 shows views (a top view, a side view, an AA section view and an enlarged section view of a B section) of a composite state of the electrophoresis medium container. 105 . Fig. Figure 5 shows views (a sectional view, an enlarged partial sectional view) of a usage state of the electrophoresis medium container. 105 .

[0077] As in Fig. As shown in section 3, the electrophoresis medium container includes 105 according to the present embodiment, for example a polycarbonate container 214 , a silicone rubber partition 215 and a polycarbonate cover 216 The container 214 and the cover 216 are not limited to polycarbonate, but can be made of polypropylene, a COP resin, PMMA, or similar materials. Additionally, the partition wall 215not limited to silicone rubber and can consist of fluorocarbon rubber, EPDM rubber or the like.

[0078] The container 214 It has eight holes spaced 9 mm apart horizontally. The holes have a diameter ϕ of 1.5 mm and a depth of 5.1 mm, and each hole has an internal volume that contains approximately 9 μl of the electrophoresis medium.

[0079] The partition wall 215 is attached by placing it between the container 214 and the cover 216 is arranged. In addition to individual shaping, the partition wall can 215 by means of two-tone shapes or the like, integrally with the cover 216 to be shaped.

[0080] Fig. Figure 4 shows a state in which the electrophoresis medium container 105 from the disassembled state in Fig. 3 was assembled. According to the present embodiment, the container214 and the cover 216 by fitting a snap-fit ​​clamp 217 , which are in the container 214 is provided, into a snap-in seat hole 218 , which is in the cover 216 The provided components fit together perfectly. In addition to a snap-fit ​​design, the container can be used for various purposes. 214 and the cover 216 Use fasteners such as adhesives or ultrasonic bonding agents. At this time, a container is used. 214 provided pen section 219 into a positioning hole 220 introduced, which is in the cover 216 is provided, and then a capillary cathode end insertion hole is created. 221 positioned. A clear lead. 403 is in the container 214 trained, and he will be involved in attaching the electrophoresis medium container. 105 used as described below.

[0081] Fig. Figure 5 shows a usage state of the electrophoresis medium container. 105 , which, as in Fig. 4 was presented as a composite. As in Fig. As shown in section 5, this is an electrophoresis medium. 222 in the container 214 of the electrophoresis medium container 105 housed. The capillary cathode end 207 the capillary 101 penetrates the partition wall 215 The capillary 101 and the partition 215 are arranged in the same way.

[0082] A partition wall structure 215 will be with reference to the Fig. 6 and Fig. 7 described. Fig. Figure 6 shows views (a top view, an AA section view and an enlarged section view of a B section) of a partition wall structure. 215 . Fig. Figure 7 shows views (a sectional view of the cover, an enlarged sectional view of an A-section of the cover, and a sectional view of the partition and the container) of a structure of the electrophoresis medium container. 105 .

[0083] As in Fig. As shown in section 6, the partition wall 215 in the middle section a more detailed section 301 , so that the capillary cathode end 207 easily passes through it. Additionally, the partition wall 215 a tapered section 302 to the in-depth section 301 , so that an external force normal to a penetration section of the capillary cathode end 207 is exercised. The cover 216 , as in Fig. As shown in section 7, a tapered part 303 The tapered part 303 the cover 216 and the tapered part 302 the partition wall 215are provided at appropriate positions so that they can be brought into contact with each other in a state of use at a predetermined pressure. Accordingly, as in Fig. Figure 7 is shown, which is in the cover 216 provided incoming part 303 in contact with the partition wall 215 provided incoming part 302 , thereby exerting an external force normal to the penetration section of the capillary cathode end 207 is exercised.

[0084] The external force acts in an orientation in which the opened hole is oriented during the penetration of the capillary cathode end. 207 is closed, so that the opened hole comes into close contact with the capillary cathode end. 207 The higher the internal pressure of the electrophoresis medium container, the more... 105 The greater the increase in height, the greater the increase in external force. This is also effective in a case where the capillary cathode end207 from the partition wall 215 is pulled out so that no liquid leakage occurs because the hole is closed by the external force. Furthermore, the outer circumference 304 the partition wall 215 The structure of an O-ring. When the cover 216 When installed, a gap is created in the structure between the partition wall. 215 and the container 214 through a crushed edge of the outer circumference 304 filled, and this prevents the electrophoresis medium from 222 It evaporates and leaks. <Betrieb der Gesamtelektrophoresevorrichtung>

[0085] Next, a series of processing operations of the electrophoresis device according to the present embodiment are described. Furthermore, a drive process of the autosampler is described. 203or the process of applying a voltage to perform electrophoresis in the electrophoresis device, as described below, is realized by a control unit (for example, a computer) (not shown).

[0086] The Fig. 8, Fig. 9, Fig. 10 and Fig. Figure 11 shows processing steps that are carried out when the capillary arrangement 201 with the electrophoresis medium 222 is filled. Fig. Figure 8 shows views (a top view, a side view and an AA sectional view) of a receiving section of the electrophoresis medium container. 105 . Fig. Figure 9 is a view (perspective view) of the installation of the electrophoresis medium container. 105 . Fig. Figure 10 shows views (a top view, a side view, an AA section view and an enlarged section view of a section) of an installation state of the electrophoresis medium container.105 . Fig. Figure 11 shows views (a sectional view and an enlarged sectional view of a C-section) of a filling state with an electrophoresis medium.

[0087] First, the electrophoresis medium container is 105 in the recording section 401 the shell 208 installed. Fig. 8 shows the recording section 401 the shell 208 , and Fig. Figure 9 shows a state in which the electrophoresis medium container 105 in the recording section 401 is installed. An electrophoresis medium container positioning hole. 402 will be in the recording section 401 the shell 208 opened, with the introduction of the electrophoresis medium container 105 into the hole 402 allows the electrophoresis medium container 105 accurate and repeatable with respect to the capillary 101 is positioned.

[0088] Additionally, a round lead plays a role. 403 , which is located on an outer circumference of a cylindrical section of the electrophoresis medium container 105 is positioned to play the role of a snap-in seat, as in Fig. Figure 10 shows that a holding force is exerted against an upward frictional force generated when the capillary cathode end... 207 is pulled out so that the electrophoresis medium container 105 is attached.

[0089] Next, the bowl 208 through the autosampler 203 driven in a horizontal direction, and the recessed section 301 of the electrophoresis medium container 105 is located at a position below the capillary cathode end 207 arranged.

[0090] Then the shell 208 through the autosampler 203 The partition wall is lifted 215 of the electrophoresis medium container 105penetrates and becomes the capillary cathode end 207 in the electrophoresis medium container 105 introduced. Fig. Figure 11 shows a state after the introduction of the capillary cathode end. 207 Because there's a hole like that in the partition wall 215 It is opened so that its shape is similar to that of the capillary cathode end. 207 If it matches, the capillary cathode end can be identified. 207 and the partition 215 slightly dense.

[0091] Additionally, an external force is applied normal in the direction of a position of the partition wall. 215 exercised where the capillary cathode end 207 penetrates. The external force acts in an orientation in which the opened hole is oriented during the penetration of the capillary cathode end. 207 is closed. Accordingly, during the penetration of the capillary cathode end, 207 the opened hole and the capillary cathode end 207They are brought into close contact with each other. In this way, a leak from the penetration section of the capillary cathode end is prevented. 207 prevented.

[0092] When the capillary cathode end 207 If it has an outer diameter of 0.71 mm, its pressure resistance can be approximately 8 MPa.

[0093] As a result of the sealing of the partition wall 215 The electrophoresis medium 222 in the electrophoresis medium container 105 by the amount of the insertion volume of the capillary cathode end 207 compressed, so that pressure is created to cause the interior of the capillary to expand. 101 with the electrophoresis medium 222 is filled. The liquid supply pressure with which enough electrophoresis medium is supplied. 222 The injected pressure is greater than or equal to approximately 3 MPa. The introduced volume of the capillary cathode end 207 is larger than the internal volume of the capillary 101In this way, the electrophoresis medium is 222 from the electrophoresis medium container 105 into the interior of the capillary 101 introduced.

[0094] For example, in a case where the inner diameter of the capillary is 4, penetration 101 The length of the capillary is 50 μm. 101 360 mm and the outer diameter 4) of the capillary cathode end 207 the capillary 101 The capillary cathode end measures 0.71 mm 207 through the partition wall 215 and is then inserted 3.67 mm, and a quantity twice the internal volume of the capillary can be supplied. 101 The fluid supply pressure is reduced when the capillary 101 with the electrophoresis medium 222 The capillary is filled. The filling rate of the capillary responds to a reduction in the fluid supply pressure. 101 with the electrophoresis medium 222The insertion state is reduced accordingly approximately one to two minutes after insertion of the capillary cathode end. 207 retained, thereby preserving the interior of the capillary 101 with a quantity of the electrophoresis medium 222 is filled, which corresponds to the introduced volume of the capillary cathode end 207 closer.

[0095] When the capillary is being filled 101 with the electrophoresis medium 222 Once completed, the shell 208 through the autosampler 203 transported downwards, and the capillary cathode end 207 is taken from the electrophoresis medium container 105 pulled out.

[0096] Then the autosampler transports 203 the shell 208 , and the capillary cathode end 207 is immersed in the sample, which is in the sample container 210 is contained, immersed in pure water (for cleaning), which is in a pure water container209 is contained, and immersed in a buffer solution contained in buffer solution container B 109 is included, and this is done in this order.

[0097] Electrophoresis is initiated in a state where the capillary cathode end 207 is immersed in the buffer solution. Furthermore, the capillary anode end (capillary head) is 204 ) immersed in the buffer solution contained in buffer solution container A 106 is contained before a series of processing operations are initiated. In this way, electrophoresis is performed in a state where both ends of the capillary are directly immersed in the buffer solution. Additionally, during the analysis of the sample, the sample is subjected to electrophoresis in the electrophoresis medium, and the difference in electrophoresis rates is measured by the detector. 108 detected.

[0098] After completing the electrophoresis, the user removes the electrophoresis medium container. 105 from the electrophoresis device and discards the electrophoresis medium container in its current state. Accordingly, the user touches the electrophoresis medium. 222 not directly, and the electrophoresis medium 222 is not attached to the electrophoresis device. <Wirkungen von Ausführungsform 1>

[0099] We previously included in the electrophoresis device according to the present embodiment the electrophoresis medium container. 105 the partition wall 215 as a sealing element, which seals the container 214 in a sealed state with an electrophoresis medium 222 It remains filled and which is from the capillary cathode end 207 can be penetrated. The electrophoresis medium 222 can be caused by the pressure that is generated when the capillary cathode end 207 the partition wall215 penetrates, which the container 214 seals, which uses the electrophoresis medium 222 is filled, into the interior of the capillary 101 be introduced.

[0100] In this case, the electrophoresis medium can 222 by the pressure that is generated when the electrophoresis medium 222 by the amount of the insertion volume of the capillary cathode end 207 into the container 214 is compressed, thereby reducing the pressure in the container 214 is increased, into the interior of the capillary 101 be introduced. Because the partition wall 215 If it is made from a rubber material that is likely to deform elastically, the sealed state in the container may be affected. 214 maintained by elastic deformation even when the capillary cathode end 207 through which it passes. Additionally, even when the capillary cathode end is pulled out, 207 from the container214 the sealed state in the container 214 be maintained.

[0101] When the capillary 101 with the electrophoresis medium 222 When filled, the electrophoresis medium container can 105 Therefore, maintain the resting state, and it is easily possible to access the capillary. 101 and the electrophoresis medium container 105 to seal. Additionally, the shape of the electrophoresis medium container 105 simplified, so that the electrophoresis medium container 105 is easy to produce and the electrophoresis medium 222 easily enclosed. Furthermore, the amount of electrophoresis medium in the container can be adjusted. 105 enclosed electrophoresis medium 222 The amount of electrophoresis medium can be approached indefinitely, thus allowing the capillary to be used in a variety of ways. 101 is filled, so that the dead volume can be reduced. [Version 2]

[0102] An electrophoresis device according to the present embodiment is described with reference to Fig. 12 described. The present embodiment is described with a focus on differences compared to the embodiment 1 described above.

[0103] In the capillary electrophoresis device according to the embodiment described above, the partition wall consists of 215 of the electrophoresis medium container 105 made of a rubber material. As long as the penetration position of the capillary cathode end 207 While the seal is achieved, rubber does not necessarily have to be used. The capillary electrophoresis device according to the present embodiment uses an example where the partition 215 of the electrophoresis medium container 105 It consists of a resin material, and it is referred to as Fig. 12 described.

[0104] Fig. Figure 12 shows views (a sectional view and an enlarged sectional view of a section) of the electrophoresis medium container. 105 according to the present embodiment. As in Fig. As shown in 12, the components of the electrophoresis medium container consist of 105 , the entire container 214 and the entire coverage 216 made of resin and is the capillary cathode end insertion section. 501 thinner than the resin. Additionally, the capillary cathode end has 207 a sharp shape. The capillary cathode end 207 penetrates the resin of the capillary cathode end insertion section 501 and is introduced into it. After introduction, the electrophoresis medium container 105 and the capillary cathode end 207 sealed, because a hole that matches the shape of the capillary cathode end 207This corresponds to the opening in the resin. In this way, unlike the embodiment described above, the electrophoresis device according to the present embodiment does not require a partition. This reduces the number of components and allows the electrophoresis medium container to be used more efficiently. 105 can be produced at low cost. [Version 3]

[0105] An electrophoresis device according to the present embodiment is described with reference to Fig. 13. The present embodiment is described with a focus on the differences compared to the embodiments 1 and 2 described above.

[0106] In the capillary electrophoresis device according to the embodiment described above, the holes through which the electrophoresis medium passes are 222 of the electrophoresis medium container 105The capillary electrophoresis device according to the present embodiment uses an example where the holes through which the electrophoresis medium is enclosed are provided individually. However, it is not necessary to provide the holes individually; rather, the respective holes can be continuously formed as a single hole. 222 of the electrophoresis medium container 105 Included are continuous, as with reference to Fig. 13 is described.

[0107] Fig. Figure 13 shows views (a top view and an AA sectional view) of a configuration of the electrophoresis medium container. 105 according to the present embodiment. As in Fig. Figure 13 shows the holes through which the electrophoresis medium passes. 222 of the electrophoresis medium container 105 is included through a communication section 601 inside the container 214continuously formed. In this way, the electrophoresis medium can be contained in the electrophoresis device according to the present embodiment. 222 in the electrophoresis medium container 105 It can be carried out differently than in the embodiment described above. [Version 4]

[0108] An electrophoresis device according to the present embodiment is described with reference to Fig. 14 described. The present embodiment is described with a focus on differences compared to the embodiments 1 to 3 described above.

[0109] In the capillary electrophoresis device according to the embodiment described above, the number of partitions separating the capillary cathode end is 207 surrounded, exactly as large as the number of capillary cathode ends 207However, it is not necessary to divide the partition into the same number of partitions as the number of capillary cathode ends. 207 , but the respective partition walls 215 can be continuously formed together. The capillary electrophoresis device according to the present embodiment uses an example in which the respective partitions 215 are continuously trained, as with reference to Fig. 14 is described.

[0110] Fig. Figure 14 is a view (perspective single-part view) of a configuration of the electrophoresis medium container. 105 according to the present embodiment. As in Fig. As shown in 14, the respective partition walls are 215 between the container 214 and the cover 216continuously formed. In this way, the number of components of the electrophoresis medium container can be continuously increased in the electrophoresis device according to the present embodiment. 105 compared to the embodiment described above. [Version 5]

[0111] An electrophoresis device according to the present embodiment is described with reference to the Fig. 15 to Fig. 18 described. The present embodiment is described with a focus on differences compared to the embodiments 1 to 4 described above.

[0112] In the capillary electrophoresis device according to the embodiment described above, the case is described in which the capillary 101 by the pressure that is generated when each capillary cathode end 207 in the electrophoresis medium container 105 is introduced with the electrophoresis medium 222is filled. Before the capillary cathode end 207 in the electrophoresis medium container 105 However, the pressure in the electrophoresis medium container can be increased when introduced. 105 The capillary electrophoresis device according to the present embodiment uses an example in which the pressure in the electrophoresis medium container is increased. 105 is increased before the capillary cathode end 207 in the electrophoresis medium container 105 is introduced as described below.

[0113] The basic configuration of the capillary electrophoresis device according to the present embodiment is similar to that of the embodiments described above. Components that differ from those according to the embodiments described above are described below with reference to the drawing. <Struktur des Elektrophoresemediumbehälters>

[0114] Fig. Figure 15 shows views (a top view and an AA sectional view) of a configuration of the electrophoresis medium container. 105 according to the present embodiment. As in Fig. As shown in 15, the electrophoresis medium container 105 similar to the embodiment 1 described above, as a feature according to the present embodiment the cover 216 , the partition 215 and the container 214 Additionally, the electrophoresis medium container has 105 a terminal block 804 on, which is used to hold the electrophoresis medium container 105 to attach. A piston is involved. 801 , which is used for a syringe or the like, on the bottom side of the electrophoresis medium container 105 provided. <Betrieb der Gesamtelektrophoresevorrichtung>

[0115] Fig. Figure 16 shows an overview of an electrophoresis device according to the present embodiment. Fig. Figure 16 shows an installation state of the electrophoresis medium container. 105 during electrophoresis. Furthermore, a drive process of the autosampler is described. 203 , a process of applying a voltage for electrophoresis or the like in the electrophoresis device described below, using a control unit (for example, a computer) (not shown). A characteristic feature of the embodiment described above is that a spring piston 802 in the recording section 401 the shell 208 is provided.

[0116] The Fig. 17 and Fig. Figure 18 shows processing steps when the capillary arrangement 201 with the electrophoresis medium 222 is filled. Fig. Figure 17 is a view (sectional view) showing the installation process of the electrophoresis medium container. 105 shows. Fig. 18 is a view (sectional view) showing a state of a filling with an electrophoresis medium 222 shows.

[0117] First, the electrophoresis medium container is 105 in the recording section 401 the shell 208 installed. Fig. Figure 17 shows a state in which the electrophoresis medium container 105 in the recording section 401 is installed. The number in the recording section 401 provided spring piston 802 is exactly as large as the number of pistons 801 of the electrophoresis medium container 105 The electrophoresis medium container 105 It is installed in such a way that the spring pistons 802 in contact with the pistons 801 of the electrophoresis medium container 105 to get there. The spring pistons play a role in this.802 the role of a positioning pin and the electrophoresis medium container is located 105 at any position.

[0118] Additionally, the spring pistons 802 an external force on the pistons 801 of the electrophoresis medium container 105 exercised, and the electrophoresis medium 222 in the electrophoresis medium container 105 enters a pressurized state. The clamping section 804 is on the side surface of the electrophoresis medium container 105 Provided. A terminal section. 803 , which is in the recording section 401 Once provided, it will be placed in the terminal block section. 804 fitted, thereby the electrophoresis medium container 105 at the recording section 401 is attached. During attachment, a spring piston is used. 802The generated force is fixed on a force that is greater than the frictional force generated when the capillary cathode end 207 is pulled out.

[0119] Next, the bowl 208 through the autosampler 203 driven in a horizontal direction, and the recessed section 301 of the electrophoresis medium container 105 is located at a position below the capillary cathode end 207 arranged.

[0120] Then the shell 208 through the autosampler 203 The partition wall is lifted 215 of the electrophoresis medium container 105 penetrates and becomes the capillary cathode end 207 in the electrophoresis medium container 105 introduced. Fig. Figure 18 shows the state immediately after insertion. The sealing structure between the capillary cathode end 207 and the partition 215and the operation of the device is the same as that according to embodiment 1.

[0121] In this way, unlike in the embodiment described above, the capillary can be 101 in the electrophoresis device according to the present embodiment, even then with the electrophoresis medium 222 will be filled when the introduced volume of the capillary cathode end 207 is smaller than the internal volume of the capillary 101 In other words, the outer diameter of the capillary cathode end can be 207 can the insertion length of the capillary cathode end be reduced, or can it be reduced? 207 be shortened. [Version 6]

[0122] An electrophoresis device according to the present embodiment is described. The present embodiment is described with a focus on the differences compared to the embodiments 1 to 5 described above.

[0123] In the capillary electrophoresis device according to the embodiment described above, the internal pressure of the container is increased by an external pressure source from outside the electrophoresis medium container. 105 The applied force is increased. However, the internal pressure can be adjusted using properties of the electrophoresis medium. 222 The capillary electrophoresis device according to the present embodiment uses an example in which the internal pressure is increased using the properties of the electrophoresis medium. 222 is increased.

[0124] For example, when the electrophoresis medium 222 in the electrophoresis medium container 105 The electrophoresis medium is enclosed in a container with a low temperature. 105 It only returns to room temperature when the user begins using it. At that time, the volume of the electrophoresis medium will increase. 222due to the thermal expansion of the electrophoresis medium 222 The pressure in the sealed space is increased, generating a pressure above atmospheric pressure. In this way, unlike the embodiment described above, the internal pressure in the electrophoresis device according to the present embodiment can be adjusted using the properties of the electrophoresis medium. 222 without a mechanism or the like that exerts external pressure. [Version 7]

[0125] An electrophoresis device according to the present embodiment is described with reference to Fig. 19 described. The present embodiment is described with a focus on differences compared to the embodiments 1 to 6 described above.

[0126] In the capillary electrophoresis device according to the embodiment described above, the pressure in the electrophoresis medium container is 105increased before the capillary cathode end 207 in the electrophoresis medium container 105 is introduced. The pressure in the electrophoresis medium container 105 However, this can also occur after the introduction of the capillary cathode end. 207 The capillary electrophoresis device according to the present embodiment uses an example in which the pressure in the electrophoresis medium container is increased. 105 after the introduction of the capillary cathode end 207 is increased, and it is used with reference to Fig. 19 described.

[0127] Fig. Figure 19 is a view (sectional view) which shows a procedure for the installation of the electrophoresis medium container. 105 as shown in the present embodiment. Fig. The mechanism shown in 19 is an ejection mechanism. 901 , which the spring piston 802 the shell 208 pushes upwards, is provided, and the piston is then 801through the spring piston 802 through the ejection mechanism 901 pushed upwards after the capillary cathode end 207 in the electrophoresis medium container 105 has been introduced. In this way, in the electrophoresis device according to the present embodiment, it is possible to adjust the electrophoresis medium container. 105 It is easier to install because, unlike the embodiment described above, it does not receive a force opposite to the installation direction when a user removes the electrophoresis medium container. 105 installed. [Version 8]

[0128] An electrophoresis device according to the present embodiment is described with reference to the Fig. 20 to Fig. 23. The present embodiment is described with a focus on differences compared to the embodiments 1 to 7 described above.

[0129] In the capillary electrophoresis device according to the embodiment described above, the electrophoresis medium container 105 through the partition wall 215 kept sealed under all circumstances. Regarding the filling of the capillary. 101 with the electrophoresis medium 222 It should be noted, however, that the sealed state of the electrophoresis medium container is maintained except during storage of the electrophoresis medium container. 105 and during the filling of the capillary 101 with the electrophoresis medium 222 cannot be maintained. The capillary electrophoresis device according to the present embodiment uses an example in which the sealed state of the electrophoresis medium container cannot be maintained except during storage of the electrophoresis medium container. 105 and during the filling of the capillary 101 with the electrophoresis medium 222will not be maintained, as described below.

[0130] Furthermore, the basic configuration of the capillary electrophoresis device according to the present embodiment is similar to that of the embodiments described above. Components that differ from those according to the embodiments described above are described below with reference to the drawing. <Struktur des Elektrophoresemediumbehälters>

[0131] The Fig. 20 to Fig. Figure 23 shows detailed configurations of the electrophoresis medium container. 105 , which is used according to the present embodiment. Fig. Figure 20 is a view (perspective single-part view) of a disassembled configuration of the electrophoresis medium container. 105 . Fig. Figure 21 shows views (a top view, an AA section view and an enlarged section view of one section) of the electrophoresis medium container configuration. 105 . Fig. Figure 22 shows views (a sectional view and an enlarged sectional view of a section) of a state immediately after the introduction of the capillary cathode end. 207 . Fig. Figure 23 shows views (a sectional view and an enlarged sectional view of a section) of an introduction state of the capillary cathode end. 207 .

[0132] As in the Fig. 20 and Fig. The electrophoresis medium container shown in 21 is 105 designed to have an evaporation prevention seal 1001 and the container 214 exhibits, wherein the electrophoresis medium 222 is enclosed. An inner protrusion. 1002 , which plays a role in sealing during the insertion of the capillary cathode end 207playing is inside the container 214 provided. Additionally, the advantage is 403 similar to the embodiments described above on the outer section of the container 214 provided and is used to supply the electrophoresis medium container 105 to attach. <Betrieb der Gesamtelektrophoresevorrichtung>

[0133] Next, a series of processing operations carried out by the electrophoresis device according to the present embodiment will be described.

[0134] First, the evaporation prevention seal is installed. 1001 withdrawn before the electrophoresis medium container 105 on the shell 208 is installed. Then the electrophoresis medium container is installed. 105 in the recording section 401 the shell 208 installed.

[0135] Similar to the embodiments described above, the electrophoresis medium container 105 through the advantage 403 attached to the outer circumference of the cylindrical section of the electrophoresis medium container 105 is provided.

[0136] Next, the bowl 208 through the autosampler 203 driven in a horizontal direction, and the electrophoresis medium container 105 is located below the capillary cathode end 207 positioned.

[0137] Then the shell 208 through the autosampler 203 raised, and the capillary cathode end 207 is placed in the electrophoresis medium container 105 introduced. Fig. Figure 22 shows a state immediately after insertion. The outer circumference of the capillary cathode end. 207 comes into contact with the inner protrusion 1002 for the seal, which is in the electrophoresis medium container105 is provided. In other words, the electrophoresis medium container is available. 105 at this point it enters a sealed state. The diameter of the inner projection 1002 The sealing effect is less than that of the capillary cathode end. 207 .

[0138] Fig. 23 shows a state in which the shell 208 is further raised from the state described above. The capillary cathode end 207 comes into contact with the inner protrusion 1002 of the electrophoresis medium container 105 , and the sealed state is maintained. Accordingly, the electrophoresis medium 222 by introducing the capillary cathode end 207 When compressed, the pressure in the electrophoresis medium container is reduced. 105 increased and the capillary becomes 101 thereby with the electrophoresis medium 222 filled.

[0139] The process during electrophoresis is the same as in the embodiments described above.

[0140] In this way, unlike in the embodiment described above, the electrophoresis device according to the present embodiment can maintain the sealed state of the electrophoresis medium container. 105 cannot be maintained, except during storage of the electrophoresis medium container. 105 and during the filling of the capillary 101 with the electrophoresis medium 222 . [Version 9]

[0141] An electrophoresis device according to the present embodiment is described with reference to Fig. 24 described. The present embodiment is described with a focus on differences compared to the embodiments 1 to 8 described above.

[0142] In the capillary electrophoresis device according to the embodiment described above, the inner projection 1002 for sealing, in order to enter the sealed state, in the electrophoresis medium container 105 trained.

[0143] However, it can also be an advantage 1003 in the capillary cathode end 207 The capillary electrophoresis device according to the present embodiment uses an example in which the projection 1003 in the capillary cathode end 207 is trained, as with reference to Fig. 24 is described.

[0144] Fig. Figure 24 shows views (a perspective view and an enlarged sectional view of a section) of the capillary arrangement. 201 according to the present embodiment. As in Fig. The advantage shown in 24 is 1003 on the outer circumference of the capillary cathode end 207provided. The advantage 1003 has an outer diameter that is larger than the diameter of the hole into which the capillary cathode end is inserted. 207 of the electrophoresis medium container 105 is introduced. In this way, the electrophoresis medium container can be inserted in the electrophoresis device according to the present embodiment. 105 They can be manufactured easily because, unlike the embodiment described above, it is not necessary to create the inner projection. 1002 on the side of the electrophoresis medium container 105 to form. [Version 10]

[0145] An electrophoresis device according to the present embodiment is described with reference to the Fig. 25 and Fig. 26. The present embodiment is described with a focus on differences compared to the embodiments 1 to 9 described above.

[0146] In the capillary electrophoresis device according to the embodiment described above, each capillary cathode end 207 with the electrophoresis medium 222 filled. However, it is also possible to change the capillary arrangement. 201 from the anode side with the electrophoresis medium 222 to fill. The capillary electrophoresis device according to the present embodiment uses an example of an electrophoresis device with a structure that serves to fill the electrophoresis medium. 222 from a capillary anode end 1103 to fill as described below.

[0147] Furthermore, the basic configuration of the capillary electrophoresis device according to the present embodiment is similar to that of the embodiments described above. Components that differ from those according to the embodiments described above are described below with reference to the drawing. <Struktur der Kapillarenanordnung>

[0148] Fig. 25 is a view (perspective view) of the capillary arrangement 201 according to the present embodiment. As in Fig. Figure 25 shows the characteristic capillary arrangement. 201 according to the present embodiment, the configuration (charging head 205 and capillary cathode end 207 ) on the cathode side of the capillary arrangement 201 that in the embodiments described above. The capillary arrangement is... 201 similar to the cathode side, but with an anode-side charging head. 1101 Equipped with a tubular anode. 1102 is in the anode-side charging head 1101 provided. The capillary 101 penetrates the anode 1102 and protrudes from the lower end of the anode 1102 before (hereinafter referred to as capillary anode end) 1103 designated). <Overview of an electrophoresis device>

[0149] Fig. Figure 26 shows an overview of an electrophoresis device according to the present embodiment. A configuration of the device is described below with reference to… Fig. 26 described.

[0150] The filling of the capillary arrangement 201 with the electrophoresis medium 222 and the structure of the electrophoresis medium container 105 similar to those according to the embodiment 1 described above. However, unlike the embodiment 1 described above, there are two autosamplers. 203 , which transport the electrophoresis medium or the like, are provided at two positions on the cathode side and on the anode side.

[0151] On an anode-side shell 1104 are the buffer solution container A 106 , which contains the buffer solution into which the capillary anode end 1103is immersed, the pure water container 209 , which contains pure water for cleaning the capillary, and the electrophoresis medium container 105 , which contains the electrophoresis medium, is mounted.

[0152] An anode-side autosampler 1105 is designed to have two timing belts 211 exhibits, similar to the embodiments described above, the right-left direction (horizontal direction: X) or the up-down direction (vertical direction: Y) in Fig. 26 correspond. The rotation of the two timing belts 211 It allows the anode-side shell 1104 Transport in a right-left direction and in an up-down direction. Transport in two axial directions allows the respective containers, which are located on the anode-side shell, to be transported in the correct direction. 1104 are mounted at one of the capillary anode ends 1103They are mounted in the opposite position. Furthermore, the timing belts 211 by the rotation of a motor 213 driven by a pulley 212 is connected.

[0153] On the cathode-side shell 208 are the buffer solution container B 109 , which contains the buffer solution into which the capillary cathode end 207 is immersed, the pure water container 209 , which contains pure water for cleaning the capillary, and the sample container 210 , which contains the sample, is assembled. <Betrieb der Gesamtelektrophoresevorrichtung>

[0154] Next, a series of processing operations of the electrophoresis device according to the present embodiment are described. Furthermore, a drive operation of the autosampler or the operation of applying a voltage to perform electrophoresis in the electrophoresis device, as described below, is implemented by a control unit (for example, a computer) (not shown).

[0155] The following are the processing steps involved in filling the capillary assembly. 201 with the electrophoresis medium 222 described.

[0156] First, the electrophoresis medium container is 105 in the recording section 401 the anode-side shell 1104 installed. The structure of the recording section 401 the anode-side shell 1104 is similar to that according to the embodiment described above 1.

[0157] Next, the anode-side shell will be1104 through the anode-side autosampler 1105 driven in a horizontal direction, and the recessed section of the electrophoresis medium container 105 is located at the position below the end of the capillary anode. 1103 positioned.

[0158] Then the anode-side shell 1104 through the anode-side autosampler 1105 raised. The capillary anode end 1103 penetrates the partition wall 215 of the electrophoresis medium container 105 and is placed in the electrophoresis medium container 105 introduced, thereby opening the capillary 101 with the electrophoresis medium 222 is filled. At this time, the capillary cathode end 207 into the clean water tank 209 contained pure water.

[0159] When the capillary is filled 101 with electrophoresis medium 222 Once completed, the anode-side shell will be 1104through the anode-side autosampler 1105 transported downwards and becomes the capillary anode end 1103 from the electrophoresis medium container 105 pulled out.

[0160] Then the anode-side autosampler transports 1105 the anode-side shell 1104 , and the capillary anode end 1103 is added to the clean water (for cleaning) that is in the clean water tank 209 is contained, and into the buffer solution, which is in buffer solution container A 106 is contained, immersed, and this happens in this order.

[0161] Regarding the capillary cathode end 207 It should be noted that the capillary anode end 1103 is immersed in the buffer solution, which is in the buffer solution container A 106 is contained, and that at the same time the capillary cathode end is inserted into the sample, which is in the sample container 210 is contained, and into the buffer solution, which is in buffer solution container B109 is contained, is immersed, and this happens in this order.

[0162] Electrophoresis is initiated in a state in which both the capillary anode end 1103 as well as the capillary cathode end 207 have been immersed in the buffer solution.

[0163] In this way, unlike in the embodiment described above, it is possible in the electrophoresis device according to the present embodiment to 101 from the capillary anode end 1103 with the electrophoresis medium 222 to fill. [Version 11]

[0164] An electrophoresis device according to the present embodiment is described with reference to Fig. 27. The present embodiment is described with a focus on differences compared to the embodiments 1 to 10 described above.

[0165] In the capillary electrophoresis device according to the embodiment described above, the electrophoresis medium container 105 used, where the number of electrophoresis medium containment units is exactly the same as the number of capillaries used. 101 However, the number of electrophoresis medium containment units does not need to be exactly the same as the number of capillaries. 101 The capillary electrophoresis device according to the present embodiment uses an example in which the several bundled capillaries 101 in the electrophoresis medium container 105 be introduced, as with reference to Fig. 27 is described.

[0166] Fig. Figure 27 shows views (a sectional view and an enlarged sectional view of a C-section) of a state of a filling with the electrophoresis medium. 222 according to the present embodiment. As in Fig. 27 shows the end of the capillary anode 1103 using an adhesive in a metal tube 1201 bundled. When the capillary 101 with the electrophoresis medium 222 When filled, the several (for example, eight) bundled capillaries are used, similar to the embodiments described above. 101 together with the pipe 1201 in the electrophoresis medium container 105 In this way, the multiple bundled capillaries are introduced in the electrophoresis device according to the present embodiment. 101 in the electrophoresis medium container 105 introduced, thereby filling the capillaries 101 with the electrophoresis medium 222 This can be done more easily than in the embodiment described above. [Version 12]

[0167] An electrophoresis device according to the present embodiment is described with reference to Fig. 28 described. The present embodiment is described with a focus on differences compared to the embodiments 1 to 11 described above.

[0168] In the capillary electrophoresis device according to the embodiment described above, each capillary 101 with the electrophoresis medium 222 The capillary is filled and the electrophoresis is performed. 101 However, it can be used with the electrophoresis medium 222 to be filled. The capillary electrophoresis device according to the present embodiment uses an example in which any capillary 101 with the electrophoresis medium 222 is filled, as with reference to Fig. 28 is described.

[0169] Fig. Figure 28 shows an overview of an electrophoresis device according to the present embodiment. As in Fig. As shown in 28, when electrophoresis is performed, only the capillaries are affected. 101 , which are connected to the electrophoresis medium 222 The capillaries are filled and immersed in the buffer solution. 101 Electrophoresis probes, which are not used in electrophoresis, are immersed in pure water regardless of whether they are on the anode or cathode side. In a Fig. The 28 examples shown are from all capillaries 101 the capillary arrangement 201 the capillary cathode ends 207 , which are connected to the electrophoresis medium 222 are filled, immersed in the buffer solution, which is in buffer solution container B 109 is contained, and the capillary anode ends are 1103 , which are connected to the electrophoresis medium 222 are filled, immersed in the buffer solution, which is in buffer solution container A 106 is contained. The other capillary cathode ends 207, which are not used in electrophoresis, are immersed in the pure water that is in the pure water tank 209 is contained, and the other capillary anode ends 1103 , which are not used in electrophoresis, are immersed in the pure water that is in the pure water tank 209 is included.

[0170] At this time, a suitable voltage or similar is applied, which corresponds to the number of capillaries. 101 This corresponds to the capillaries used by the control unit (for example, a computer) (not shown). In contrast to the embodiment described above, the electrophoresis device according to the present embodiment makes it possible to... 101 to be used as needed, according to the number of samples analyzed.

[0171] The invention made by the present inventor has been specifically described above based on the embodiments. However, it goes without saying that the present invention is not limited to the embodiments described above and can be modified in various ways within a given scope without departing from its core concept. For example, the embodiments described above have been detailed to make the present invention easily understandable; however, the present invention is not necessarily limited to combining entire configurations described above. Furthermore, a part of a configuration of a particular embodiment can be replaced by another configuration of a different embodiment, and a configuration of one embodiment can be added to another configuration of a different embodiment.Additionally, a part of the configuration of each embodiment can be added to, removed from, or replaced by another configuration. Reference symbol list 101 capillaries 102 High-voltage source 103 Constant temperature oven 104 Electrophoresis medium filling unit 105 electrophoresis medium containers 106 Buffer solution containers A 107 Piston pump 108 Detector 109 Buffer solution container B 201 Capillary arrangement 202 receiving optical system 203 Autosampler 204 Capillary head 205 Charging head 206 Cathode 207 Capillary cathode end 208 bowl 209 Pure water tanks 210 sample containers 211 Timing belt 212 Pulley 213 Engine 214 containers 215 Partition wall 216 Cover 217 Snap-in seat clamp 218 Snap-in seat hole 219 Pen section 220 positioning hole 221 Capillary cathode end insertion hole 222 Electrophoresis medium 301 inset section 302 tapered section (partition wall) 303 tapered part (cover) 304 outer circumference 401 Recording Section 402 Electrophoresis medium container positioning hole 403 lead 501 Capillary cathode end insertion section 601 Communication section 801 pistons 802 spring pistons 803 Terminal section 804 Terminal block section 901 Ejection mechanism 1001 Evaporation prevention seal 1002 internal advantage 1003 Protrusion at the capillary end 1101 anode-side charging head 1102 Anode 1103 Capillary anode end 1104 anode-side shell 1105 anode side autosampler 1201 metal cylinders

Claims

[1] Electrophoresis medium container which has the following features: a sealing element which keeps a container body filled with an electrophoresis medium in a sealed state and which can be penetrated by the capillary, wherein the electrophoresis medium is introduced into the interior of the capillary by the pressure generated when the capillary penetrates the sealing element which seals the main body of the container filled with the electrophoresis medium. [2] Electrophoresis medium container according to claim 1, wherein the pressure generated when the capillary penetrates the sealing element is a pressure generated by increasing the pressure in the container main body by compressing the electrophoresis medium by the amount of the volume of the capillary introduced into the container main body. [3] Electrophoresis medium container according to claim 2, wherein the volume of the capillary introduced into the main body of the container is larger than the internal volume of the capillary. [4] Electrophoresis medium container according to claim 3, wherein the sealing element consists of a material which is likely to be elastically deformed and maintains the sealed state of the container main body by elastic deformation even when the capillary penetrates the element. [5] Electrophoresis medium container according to claim 4, wherein the sealing element consists of rubber or a resin which is likely to be elastically deformed. [6] Electrophoresis medium container according to claim 1, wherein the capillary is designed as a single capillary or as a capillary arrangement of an assembled set of several capillaries and wherein the sealing element is designed such that its arrangement corresponds to the configuration of the capillaries. [7] Electrophoresis medium container according to claim 5, wherein the sealing element is made of rubber and has a recessed section into which the capillary is inserted, a tapered part from which an external force is exerted in the direction of an insertion section of the capillaries, and an O-ring with which an intermediate space is filled with the main body of the container. [8] Electrophoresis medium container according to claim 5, wherein the main body of the container including the sealing element is made of resin and a section of the sealing element through which the capillary penetrates is thinner than the thickness of the resin in the other section. [9] Electrophoresis medium container according to claim 1, wherein the pressure in the main body of the container is increased before the introduction of the capillary. [10] Electrophoresis medium container according to claim 1, wherein the pressure in the main body of the container is increased after the introduction of the capillary. [11] Electrophoresis medium container according to claim 1, wherein the sealed state of the container main body is maintained only during storage of the electrophoresis medium container and during filling the interior of the capillary with the electrophoresis medium. [12] Electrophoresis medium container according to claim 1, wherein, when the interior of the capillary is filled with the electrophoresis medium, the filling with the electrophoresis medium is carried out from the cathode side of the capillary or from the anode side of the capillary. [13] Electrophoresis medium container according to claim 1, wherein when the interior of the capillary is filled with the electrophoresis medium, only a part of the entire capillary is filled with the electrophoresis medium. [14] Electrophoresis apparatus comprising the following: a capillary or a capillary arrangement as an assembled set of several capillaries, a receiving optical system that irradiates a sample in the capillary with light and detects the fluorescence of the sample, a high-voltage source that applies a high voltage to the capillary, a constant-temperature oven that keeps the capillary at a constant temperature, and a transport machine which transports several containers including a sample container and an electrophoresis medium container, wherein the electrophoresis medium container has a sealing element which keeps a container body filled with an electrophoresis medium in a sealed state and which can be penetrated by the capillary, and wherein the electrophoresis medium is introduced into the interior of the capillary by the pressure generated when the capillary penetrates the sealing element which seals the main body of the container filled with the electrophoresis medium. [15] Electrophoresis device according to claim 14, wherein the pressure generated when the capillary penetrates the sealing element is a pressure generated by an increase in the pressure in the main body of the container by compressing the electrophoresis medium by the amount of the volume of the capillary introduced into the main body of the container, and where the volume of the capillary introduced into the main body of the container is larger than the internal volume of the capillary.