City moat-like micro-hole cultivation plate

Abstract

City moat-like micro-hole cultivation plate, characterized in that it comprises a micro-hole cultivation plate main body (1), wherein the micro-hole cultivation plate main body (1) is provided with a water groove (2), wherein micro-culture holes (3) are arranged inside the water groove (2), the openings of which point upwards.

Description

A moat-type microporous culture plate Technical Field

[0001] The present application relates to the technical field of in vitro cell culture, and in particular to a moat-type microporous culture plate. Background Art

[0002] Microwell culture plates are important consumables for high-throughput culture of small amounts of cells in vitro for subsequent high-throughput testing and research. They are mainly used for in vitro cell culture.

[0003] Due to the small wells of microwell culture plates, the volume of culture fluid within the wells is low, making it prone to rapid evaporation in a constant-temperature incubator, causing changes in the state of the cultured cells and thus affecting experimental results. Existing microwell culture plates generally lack built-in humidification systems, instead relying on external humidification methods (such as a water tray at the bottom of the incubator or a mist atomizer) to humidify the culture space. While using a water tray to humidify the incubator interior is simple, prolonged exposure to warm water can easily breed bacteria and mold, contaminating the culture environment. Furthermore, because it relies on natural evaporation, it is difficult to maintain uniform humidity throughout the incubator space. This results in varying microenvironments for cells in different locations within the incubator, ultimately leading to unstable experimental results. Humidifiers typically use ultrasonic or steam generators to generate water mist, which is then dispersed throughout the incubator by a fan. While this method can achieve humidity regulation, it can easily generate excessive moisture, making it difficult to clean and prone to microbial growth and contamination. Summary of the Invention

[0004] The technical problem to be solved by the present application is to provide a moat-type microporous culture plate, which arranges the culture wells in a water filling tank, and utilizes the high specific heat capacity of water to improve the thermal insulation performance of the culture plate while ensuring the humidity of the microenvironment. This solves the current problem of instability and high risk of contamination when humidifying the microporous culture plate from the outside, and also solves the problem of excessive cooling of the microporous culture plate when operating in a non-incubator environment.

[0005] To solve the above problems, the following technical solutions are provided:

[0006] The moat-type microporous culture plate of this solution includes a microporous culture plate body, which is provided with a water injection groove. Micro culture holes are arranged inside the water injection groove, and the openings of the culture holes face upward.

[0007] By adopting the above technical solution: the water level in the water injection tank is lower than the opening of the culture well, and the water in the water injection tank surrounds the culture well to form a "moat"-like structure. When the water evaporates, water vapor is generated, thereby achieving humidification. Moreover, this humidification method is spontaneous and does not carry bacteria, thereby ensuring that the culture well will not be contaminated during the cell incubation process.

[0008] The water injection tank and the culture hole are an integrated structure.

[0009] By adopting the above technical solution, the use effect of the microporous culture plate is guaranteed, and the water in the water injection tank is prevented from crossing with the cells in the culture wells.

[0010] The culture holes arranged inside the water injection tank have equal horizontal and vertical spacings, and the horizontal and vertical spacings between the culture holes are both 9 mm.

[0011] By adopting the above technical solution, it can be adapted to standardized multi-channel pipettes and automated pipetting equipment.

[0012] The microporous culture plate body is surrounded by a frame, the bottom of the frame is provided with an annular groove, the inner edge of the annular groove is integrally connected to the microporous culture plate body, and a reinforcing rib connected to the body is provided in the annular groove.

[0013] By adopting the above technical solution: the frames around the bottom of the microporous culture plate body are hollowed out to save materials, and reinforcing ribs are used to improve the connection strength between the microporous culture plate body and the frame.

[0014] The above solution has the following advantages:

[0015] 1. In this scheme, the water level in the water injection tank is lower than the opening of the culture well. The water in the water injection tank surrounds the culture well to form a "moat" structure. When the water evaporates, water vapor is generated, which plays a humidification role. This humidification method is spontaneous and will not carry bacteria, ensuring that the culture well will not be contaminated during the incubation process.

[0016] 2. In this solution, each culture well is surrounded by water in the water tank. The high specific heat capacity of water improves the constant temperature performance of the entire culture plate, slows down the cooling rate of the culture plate during operation outside the incubator, and is more conducive to the growth of cells in the culture wells.

[0017] 3. In this solution, the microporous culture plate adopts an integrated structure to ensure the use effect of the microporous culture plate and avoid contamination caused by the cells in the culture wells and the water in the water injection tank crossing each other. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] FIG1 is a schematic diagram of the overall structure of a moat-type microporous culture plate of the present application;

[0019] FIG2 is a schematic diagram of a top view of a moat-type microporous culture plate of the present application;

[0020] FIG3 is a schematic diagram of the overall structure of a moat-type microporous culture plate of the present application.

[0021] Figure numerals: 1. microporous culture plate body; 2. water injection groove; 3. micro culture hole; 4. annular groove; 5. frame. DETAILED DESCRIPTION

[0022] The present invention will be described below with reference to the accompanying drawings 1-3 and embodiments.

[0023] In an embodiment, the moat-type microporous culture plate of the present invention is shown in FIG1 , and comprises a microporous culture plate body 1 , on which a water injection tank 2 is provided, wherein culture wells 3 are arranged inside the water injection tank 2 , and the openings of the culture wells 3 face upward. When water is injected into the water injection tank 2 , the water level in the water injection tank 2 is lower than the openings of the culture wells 3 , so that the water in the water injection tank 2 surrounds the culture wells 3 to form a structure similar to a "moat". The water generated by this structure evaporates to generate water vapor, which then spreads to the culture wells 3 to play a humidifying role. Moreover, this humidification method is spontaneous rather than artificially sprayed. Therefore, as long as the water in the water injection tank 2 is clean enough, it will not carry bacteria, thereby ensuring that the culture wells 3 will not be contaminated during the incubation process.

[0024] In order to ensure the overall use effect of the microporous culture plate, the water injection tank 2 and the culture well 3 adopt an integrated structure to avoid contamination caused by the cells in the culture well 3 and the water in the water injection tank 2 crossing each other.

[0025] As shown in FIG2 , in order to be compatible with a standardized multi-channel pipette and an automated pipetting station, the culture wells 3 arranged inside the water filling tank 2 have equal horizontal and vertical spacings, and the horizontal and vertical spacings between the culture wells 3 are both 9 mm.

[0026] Furthermore, as shown in Figure 3, the microporous culture plate body 1 is surrounded by a frame 5. An annular groove 4 is defined at the bottom of the frame 5. The inner edge of the annular groove 4 is integrally connected to the microporous culture plate body 1. A reinforcing rib is provided within the annular groove 4, which is connected to the body. Because the frame 5 around the bottom of the microporous culture plate body 1 is hollowed out to save material, the reinforcing rib is used to enhance the connection strength between the microporous culture plate body 1 and the frame 5.

[0027] The present invention has been described above in conjunction with specific embodiments, but those skilled in the art should be aware that these descriptions are exemplary and do not limit the scope of protection of the present invention. Those skilled in the art can make various variations and modifications to the present invention based on the spirit and principles of the present invention, and these variations and modifications are also within the scope of the present invention.

Claims

1. A moat-type microporous culture plate, characterized in that: The microporous culture plate comprises a main body (1) of a microporous culture plate, wherein a water injection groove (2) is provided on the main body (1), micro-culture holes (3) are arranged inside the water injection groove (2), and the openings of the culture holes face upwards.

2. A moat-type microporous culture plate as claimed in claim 1, characterized in that: The water injection groove (2) and the culture hole are an integrated structure.

3. A moat-type microporous culture plate as claimed in claim 1 or 2, characterized in that: The culture holes arranged inside the water injection tank (2) have equal horizontal and vertical spacings, and the horizontal and vertical spacings between the culture holes are both 9 mm.

4. A moat-type microporous culture plate as claimed in claim 1 or 2, characterized in that: The microporous culture plate body (1) is provided with a frame (5) around it, and an annular groove (4) is provided at the bottom of the frame (5). The inner edge of the annular groove (4) is integrally connected to the microporous culture plate body (1), and a reinforcing rib connected to the body is provided in the annular groove (4).