METHOD, SOLUTION AND KIT FOR PREPARING A TISSUE SAMPLE FOR 3D IMAGING
Patent Information
- Application Number
- DE602019064327
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2018-11-14
- Filing Date
- 2019-11-13
- Publication Date
- 2025-01-01
- Estimated Expiration
- 2039-11-13
AI Technical Summary
Conventional methods for preparing tissue samples for 3D imaging are limited by the need for excessive fixation, which reduces the quality of the sample and limits the range of antibodies that can be used for immunolabelling, making it difficult to visualize complex tissue structures and potentially missing critical lesions in large pathological specimens.
A method using a detergent and a buffer solution at a pH below 9, which allows for 3D imaging without the need for additional fixation, enabling the use of a wide range of antibodies and maintaining tissue integrity, allowing for whole and intact tissue imaging without serial sectioning.
This method facilitates high-resolution 3D imaging with a wide range of antibodies, maintaining tissue integrity and organ architecture, and is cost-effective, suitable for both fresh and archival samples, including paraffin-embedded tissues, reducing the likelihood of missing disease states and improving diagnostic accuracy.