Full-automatic QuECHERS experimental device

Abstract

The invention relates to a full-automatic QuECHERS experimental device, and relates to the technical field of sample pretreatment, the full-automatic QuECHERS experimental device comprises an extraction unit and a pipetting unit, the extraction unit comprises: a first tube rack module comprising a plurality of test tube racks; a carrying mechanical arm; the first cap screwing module comprises a tube cap clamping and lifting assembly acting on a tube cap, a tube body clamping and rotating assembly acting on a tube body and a first sliding rail assembly for conveying a test tube to a next station; the sample adding module comprises a homogeneous proton adding module and a salt pouring module; the second cap screwing module comprises a pipe body clamping and rotating assembly, a pipe cap clamping and lifting assembly and a second sliding rail assembly for realizing communication and conveying of the extraction unit and the pipetting unit; the invention discloses a pipetting mechanical arm and a gun head frame. The device is provided with the extraction unit capable of synchronously performing extraction operation and the pipetting unit capable of performing pipetting operation, so that the extraction operation and the pipetting operation are synchronously performed during pesticide residue inspection, and the experimental efficiency and the applicability of the device are effectively improved.

Description

Technical Field

[0001] The present application relates to the field of sample pretreatment, and in particular to a fully automatic QuECHERS experimental device. Background Art

[0002] The detection of pesticide residues in food has long been a difficult challenge. There are many impurities interfering and the residue content is low. Traditional extraction methods often affect the test results because they cannot separate the impurities. Due to the large number of impurities in food, the wide variety of pesticides with different physical and chemical properties, and the emergence of new pesticides, higher requirements are placed on the pretreatment of samples. In recent years, QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe), as a new extraction method, has become a new sample pretreatment technology widely used at home and abroad due to its characteristics of being fast, simple, cheap, effective, reliable and safe. It has been widely used in gas or liquid chromatography analysis of various pesticides, pharmaceuticals, and veterinary drugs.

[0003] Currently, most experimental instruments on the market that are suitable for the QuEChERS method have low processing efficiency and poor adaptability, and need to be improved. Summary of the invention

[0004] In view of the above problems, the present application is proposed to provide a fully automatic QuECHERS experimental device that overcomes the above problems or at least partially solves the above problems.

[0005] The fully automatic QuECHERS experimental device provided in this application adopts the following technical solution: A fully automatic QuECHERS experimental device, comprising an extraction unit and a pipetting unit; The extraction unit comprises, A first tube rack module, comprising a plurality of test tube racks for placing test tubes; A handling robot arm, comprising a mechanical claw for grabbing the test tube and a handling arm for carrying the test tube to a corresponding workstation; The first capping module comprises a tube cover clamping and lifting assembly for grabbing and lifting the tube cover, a tube body clamping and rotating assembly for clamping and rotating the tube body, and a first slide rail assembly capable of transporting the tube body clamping and rotating assembly to the next station; A sample adding module, located at the next station of the first capping module, comprises a homogenizer adding module for adding homogenizer and extraction liquid into the test tube and a salt pouring module for adding extraction salt into the test tube; and a vortex module, an oscillation module, and a centrifugal module for vortexing, oscillating, and centrifuging a sample tube or a purified salt tube; The pipetting unit comprises: The second capping module comprises a tube cap clamping and lifting assembly, a tube body clamping and rotating assembly, and a second slide rail assembly capable of transporting the tube body clamping and rotating assembly from the extraction unit to the liquid transfer unit or from the liquid transfer unit to the extraction unit; A pipetting robot, used to aspirate the liquid in one test tube and add it to another test tube; And a tip rack for placing the tips of the pipetting robot.

[0006] Optionally, the pipe cover clamping and lifting assembly includes: A first clamping jaw, used for clamping the tube cover; The clamping cylinder is used to drive the first clamping jaw to vertically rise and fall, and a bearing block is arranged on the top of the clamping cylinder. The first clamping jaw is vertically slidably connected to the bearing block, and a compression spring is arranged at the vertical sliding connection.

[0007] Optionally, the pipe cover clamping and lifting assembly further includes: The clamping slide rail is arranged below the clamping cylinder and is used to drive the clamping cylinder to slide horizontally.

[0008] Optionally, the tube body clamping and rotating assembly includes: A sleeve is disposed on the base plate; A rotating body, which is arranged inside the sleeve and rotatably connected thereto, and is provided with a lumen for accommodating the tube body; An airbag is arranged in the lumen and is used to abut against and clamp the tube body. When the airbag expands, the airbag abuts against the tube body, and the tube body is clamped and fixed in the lumen; when the airbag contracts, the airbag and the tube body are separated, and the tube body can be separated from the lumen; And a rotation driving assembly is used for driving the rotating body to rotate along the sleeve.

[0009] Optionally, the homogenizer adding module includes: The sorting cylinder is rotatably connected to the base plate, and a plurality of discharge holes are evenly arranged at the bottom edge along the circumferential direction thereof; The inclined plate is arranged in the sorting barrel, and an arc groove is arranged on a lower side along the circumferential direction thereof, wherein the arc groove and the barrel wall of the sorting barrel form a material discharge trough connected with the material discharge hole; A material discharge cylinder is arranged below the base plate and can be connected with the material discharge hole; And a material discharge driving assembly is used for driving the sorting barrel to rotate along the base plate, and during the rotation process, the material discharge barrel can be connected with the material discharge hole in sequence.

[0010] Optionally, the homogenizer adding module further includes a plurality of liquid adding pipes, and the plurality of liquid adding pipes are arranged along the circumference of the lower barrel.

[0011] Optionally, the salt pouring module includes: A balance wheel is rotatably connected to the base plate and is provided with a lever; A pendulum rod, arranged on the other side of the base plate and rotating along the vertical direction together with the balance wheel; A second clamping jaw is horizontally arranged at one end of the swing rod away from the balance wheel, and is used to clamp the test tube; and when the balance wheel rotates, the second clamping jaw drives the tube body to flip and tilt; A connecting plate, one end of which is located at a side of the base plate where the balance wheel is arranged and is vertically slidably connected to the base plate, a tension spring is arranged at the sliding connection, and when the balance wheel rotates, the lever can drive the connecting plate to slide vertically downward along the base plate; The other end extends to the other side of the base plate and is vertically provided with a funnel, the funnel is arranged opposite to the second clamping jaw, when the balance wheel rotates, the second clamping jaw drives the tube body to flip, and the funnel moves vertically downward along with the connecting plate, when the balance wheel rotates a certain angle, the tube body mouth is inserted into the funnel; And a salt pouring driving assembly is used for driving the balance wheel to rotate along the base plate.

[0012] Optionally, a vibration motor is provided on the rocker arm and / or the connecting plate.

[0013] In summary, the present application includes the following beneficial technical effects: 1. The device realizes the simultaneous extraction and pipetting operations during pesticide residue testing by providing an extraction unit that can perform extraction operations and a pipetting unit that can perform pipetting operations simultaneously, and the extraction unit and the pipetting operation can cooperate with each other during operation, effectively improving the experimental efficiency and the applicability of the device; 2. By setting up a dedicated transfer robot arm for test tube transfer and a dedicated pipetting robot arm for pipetting, the instrument can perform pipetting and test tube transfer operations simultaneously during the experiment, avoiding mutual interference between pipetting and test tube transfer operations, and further improving the experimental effect and applicability of the device. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 It is a schematic diagram of the overall structure of an embodiment of the present application.

[0015] Figure 2 It is a schematic diagram of the structure of the extraction unit in the embodiment of the present application.

[0016] Figure 3 It is a schematic diagram of the connection relationship between the first capping module and the sample adding module in the embodiment of the present application.

[0017] Figure 4 It is a structural schematic diagram of the first cap screwing mechanism in the embodiment of the present application.

[0018] Figure 5It is a cross-sectional view of the tube body clamping rotation assembly in the embodiment of the present application.

[0019] Figure 6 It is a structural schematic diagram of the homogenous proton addition module in the embodiment of the present application.

[0020] Figure 7 It is a schematic diagram of the structure inside the sorting cylinder in the embodiment of the present application.

[0021] Figure 8 It is a cross-sectional view of the homogenous proton addition module in the embodiment of the present application.

[0022] Fig. 9 It is a schematic diagram of the connection structure between the balance wheel and the pendulum rod in the embodiment of the present application.

[0023] Fig.10 It is a schematic diagram of the connection structure between the balance wheel and the connecting plate in the embodiment of the present application.

[0024] Fig.11 It is a schematic diagram of the working status of the salt pouring module in the embodiment of the present application.

[0025] Fig.12 It is a schematic diagram of the structure of the liquid transfer unit in the embodiment of the present application.

[0026] Explanation of reference numerals: 1. extraction unit; 11. first tube rack module; 12. handling robot arm; 13. first capping module; 131. tube cover clamping and lifting assembly; 1311. first clamping claw; 1312. clamping cylinder; 1313. bearing block; 1314. compression spring; 1315. clamping slide rail; 132. tube body clamping and rotating assembly; 1321. sleeve; 1322. rotating body; 1323. air bag; 133. first slide rail assembly; 1331. waste liquid recovery cylinder; 14. homogenizer adding module; 141. sorting cylinder; 1411. feeding hole ;142, inclined plate;1422, arc groove;143, discharge barrel;144, liquid adding tube;15, salt pouring module;151, balance wheel;1511, lever;152, rocker;153, second clamp;154, connecting plate;1541, funnel;155, tension spring;156, vibration motor;16, vortex module;17, oscillation module;18, centrifugal module;2, pipetting unit;21, second capping module;211, second slide rail assembly;22, pipetting robot arm;23, gun tip rack;24, sample bottle rack;25, gun tip disassembly assembly. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiment is one embodiment of the present invention, not all embodiments. Based on the described embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] Unless otherwise defined, technical or scientific terms used in the present invention shall have the common meanings understood by one having ordinary skills in the field to which the present invention belongs.

[0029] This embodiment provides a fully automatic QuECHERS experimental device.

[0030] Reference Figure 1 A fully automatic QuECHERS experimental device comprises a frame and an extraction unit 1 and a pipetting unit 2 arranged on the frame, wherein the extraction unit 1 is used to complete the extraction steps of the pesticide residue sample, including opening and closing the cover, adding homogenizers, adding extraction liquid, adding extraction salt, vortex mixing, oscillation mixing and centrifugation; the pipetting unit 2 is used to complete the pipetting operation, including opening and closing the cover, pipetting and supernatant membrane passing operations.

[0031] Reference Figure 1 , Figure 2 The extraction unit 1 includes a first tube rack module 11, a handling robot arm 12, a first capping module 13, a sample adding module, a vortex module 16, an oscillation module 17 and a centrifugal module 18.

[0032] The first tube rack module 11 includes a variety of test tube racks, including a sample tube rack for placing sample tubes, a purified salt tube rack for placing purified salt tubes, a finished product tube rack for placing finished product tubes, and an extraction salt tube rack for extraction salt tubes. In order to prevent the target object to be tested in the sample tube from decomposing due to heat, a sample ice bath assembly is provided under the sample tube rack.

[0033] The transport robot 12 includes a mechanical claw and a transport arm for carrying the mechanical claw. The mechanical claw is used to clamp the sample tube or the purified salt tube or the finished product tube or the extracted salt tube. The transport arm can drive the mechanical claw to move along the X direction, the Y direction, and the Z direction, and cooperate with the mechanical claw to transport the sample tube or the purified salt tube or the finished product tube or the extracted salt tube to the corresponding workstation for processing.

[0034] Reference Figure 3-Figure 5 The first capping module 13 is used to open and close the sample tube, and includes a tube cap clamping and lifting assembly 131 and a tube body clamping and rotating assembly 132 .

[0035] The pipe cover clamping and lifting assembly 131 can clamp and fix the pipe cover and drive it to lift vertically, and includes a first clamping jaw 1311 and a clamping cylinder 1312. The first clamping jaw 1311 is used to clamp and fix the pipe cover, and the clamping cylinder 1312 is used to carry the first clamping jaw 1311 and drive it to lift vertically.

[0036] The tube body clamping rotating assembly 132 is relatively arranged below the tube cover clamping lifting assembly 131, and is used to clamp the tube body and drive it to rotate, and includes a sleeve 1321, a rotating body 1322, an airbag 1323, and a rotating driving assembly. The sleeve 1321 is vertically arranged on the base plate, the rotating body 1322 is vertically arranged in the sleeve 1321 and is rotatably connected thereto, and the rotating driving assembly is used to drive the rotating body 1322 to rotate along the sleeve 1321.

[0037] The rotating body 1322 is provided with a lumen for accommodating the tube body. The airbag 1323 is provided in the lumen for contacting and clamping the tube body. In a specific implementation, the airbag is preferably a ring-shaped airbag. When the airbag 1323 is inflated, the airbag 1323 contacts the tube body. At this time, the tube body is clamped and fixed in the lumen, and the rotating body 1322 can drive the tube body to rotate together. When the airbag 1323 is deflated and contracted, the airbag 1323 and the tube body are separated, and at this time, the tube body can be separated from the lumen.

[0038] In order to realize automatic lifting and lowering control of the tube cover during opening and closing operations and reduce the movement of the clamping cylinder 1312, the tube cover clamping and lifting assembly 131 also includes a supporting block 1313 arranged on the clamping cylinder 1312, and the first clamping jaw 1311 can be vertically slidably connected to the supporting block 1313 by a telescopic rod or the like, and a compression spring 1314 is arranged at the vertical sliding connection.

[0039] The process of opening and closing the cover is as follows: when opening the cover, the clamping cylinder 1312 drives the first clamping jaw 1311 to move downward and clamp the tube cover, and at the same time inflates the airbag 1323 to clamp and fix the tube body, then the rotating body 1322 rotates, and the tube cover drives the first clamping jaw 1311 to press the compression spring 1314 upward under the action of the thread drive, and when the tube body and the tube cover are completely separated, the clamping cylinder 1312 drives the first clamping jaw 1311 to move upward, and the cover opening operation is completed; When closing the cover, the clamping cylinder 1312 drives the first clamping jaw 1311 to move downward again and make the tube cover and the tube body conflict with each other, and then the rotating body 1322 rotates in the opposite direction, and the tube cover is tightened downward under the action of the threaded drive and the compression spring 1314 is stretched downward. When the tube cover and the tube body are fully matched, the first clamping jaw 1311 is separated from the tube cover, and the clamping cylinder 1312 drives the first clamping jaw 1311 to move upward again and reset, completing the closing operation.

[0040] In order to avoid interference between the transport robot arm 12 and the tube cover clamping lifting assembly 131 when the transport robot arm 12 loads sample tubes into the tube body clamping rotating assembly 132, a clamping slide rail 1315 is further provided. The clamping slide rail 1315 is provided below the clamping cylinder 1312, and is used to drive the tube cover clamping lifting assembly 131 to slide relatively to the side of the tube body clamping rotating assembly 132 when loading sample tubes, thereby giving way to the transport robot arm 12.

[0041] The first capping module 13 further includes a first slide rail assembly 133 , which is disposed below the base plate and is used to drive the tube clamping rotating assembly 132 to slide to the next station (i.e., the sample adding module) to perform operations such as adding homogenizers, extraction liquid, and extraction salt.

[0042] Reference Figure 3 as well as Figure 6-Figure 8 The sample adding module includes a homogenizer adding module 14 and a salt pouring module 15. After the cover is opened, the sample tube first moves to the homogenizer adding module 14 along with the first slide rail assembly 133, and the homogenizer adding module 14 adds homogenizer and extraction liquid into the sample tube; then the sample tube continues to move to the salt pouring module 15 along with the first slide rail assembly 133, and the salt pouring module 15 adds extraction salt into the sample tube.

[0043] The homogenizer adding module 14 includes a sorting barrel 141, a slanting plate 142, a feeding barrel 143, a feeding drive assembly and a plurality of liquid adding pipes 144. The sorting barrel 141 is used to accommodate homogenizers and is vertically arranged on a base plate and rotatably connected to the base plate. The feeding drive assembly is used to drive the sorting barrel 141 to rotate along the base plate. A plurality of feeding holes 1411 are evenly arranged at the bottom edge of the sorting barrel 141 along its circumferential direction. Each feeding hole 1411 can accommodate a homogenizer. The inclined plate 142 is arranged in the sorting barrel 141, and an arc groove 1422 is arranged on its lower side along its circumferential direction. The arc groove 1422 and the barrel wall of the sorting barrel 141 form a material discharge trough, and the material discharge trough is connected with the material discharge hole 1411. Under the action of the inclined surface of the inclined plate 142, the homogenizers can automatically enter the material discharge hole 1411.

[0044] The discharge barrel 143 is arranged below the substrate and passes through the substrate. During the rotation of the sorting barrel 141, the discharge barrel 143 can be connected with the discharge holes 1411 arranged at the bottom of the sorting barrel 141 in sequence, and the homogenizers enter the sample tube through the discharge barrel 143 to realize automatic addition of the homogenizers.

[0045] Several liquid adding pipes 144 are evenly arranged along the circumferential direction of the lower barrel 143, and the extraction liquid can be added synchronously when the homogenizer is added, effectively improving the addition efficiency of the extraction material. At the same time, in order to facilitate the liquid adding pipe 144 to discharge air or perform self-cleaning, a waste liquid recovery cylinder 1331 is also provided on the first slide rail assembly 133.

[0046] Reference Figure 3 as well as Figure 9-11 The salt pouring module 15 includes a balance wheel 151, a swing rod 152, a second clamping claw 153, a connecting plate 154 and a salt pouring driving assembly. The balance wheel 151 is rotatably connected to the base plate, and the salt pouring driving assembly is used to drive the balance wheel 151 to rotate along the base plate.

[0047] The swing rod 152 is arranged on the other side of the base plate and connected to the balance wheel 151, and rotates along the vertical direction with the balance wheel 151. The second clamping jaw 153 is horizontally arranged at one end of the swing rod 152 away from the balance wheel 151, and is used to clamp the extraction salt tube, and when the balance wheel 151 rotates, the swing rod 152 and the second clamping jaw 153 rotate together, and the extraction salt tube clamped by the second clamping jaw 153 performs a flipping and tilting motion, that is, the bottom of the tube flips upward, and the tube mouth flips downward.

[0048] One end of the connecting plate 154 is located on the same side of the substrate as the balance wheel 151, and is vertically slidably connected to the substrate, and a tension spring 155 is provided at the vertical sliding connection, and the tension spring 155 is used to drive the connecting plate 154 to reset; a lever 1511 is provided at the edge of the balance wheel 151, and when the balance wheel 151 rotates, the lever 1511 can drive the connecting plate 154 to slide vertically downward along the substrate; the other end of the connecting plate 154 extends to the other side of the substrate and is vertically provided with a funnel 1541, which is arranged opposite to the second clamp 153 and is used to be inserted into the sample tube when adding extraction salt.

[0049] When adding salt, the transport robot arm 12 transports the extraction salt tube to the second clamping jaw 153, and the second clamping jaw 153 clamps the extraction salt tube. The transport robot arm 12 moves upward, and the tube cover and the tube body of the extraction salt tube are separated, and the extraction salt tube is opened. At this time, the opened sample tube moves with the first slide rail assembly 133 to the bottom of the funnel 1541; Then, the salt pouring driving assembly drives the balance wheel 151 to rotate. When the balance wheel 151 rotates, the second clamping jaw 153 drives the extraction salt tube to flip, and at the same time, the funnel 1541 slides vertically downward together with the connecting plate 154. When the balance wheel 151 rotates a certain angle, the sample tube is in a downward tilting state and the tube mouth is inserted into the funnel 1541. At this time, the funnel 1541 is also inserted into the sample tube to realize the salt adding action; After the salting is completed, the balance wheel 151 rotates in the opposite direction to return to the initial state. At this time, the second clamping jaw 153 and the funnel 1541 also return to the initial state. The connecting plate 154 slides upward and automatically resets under the drive of the tension spring 155 to prepare for the next salting.

[0050] In order to prevent the extracted salt from adhering to the wall of the extracted salt pipe or the inner wall of the funnel 1541 during the salt addition process, a vibration motor 156 is provided on the swing rod 152 and the connecting plate 154. At the same time, in order to facilitate the disposal of the waste pipe of the extracted salt pipe, a recovery port located below the second clamping jaw 153 is provided on the frame. After the salt addition is completed, the second clamping jaw 153 is released, and the waste pipe can automatically fall into the recovery port.

[0051] The vortex module 16, the oscillation module 17 and the centrifugal module 18 are arranged in sequence, and are used to vortex, oscillate and centrifuge the sample tube or the purified salt tube, respectively. (The vortex module 16, the oscillation module 17 and the centrifugal module 18 are existing mature technologies, and their specific structures will not be described in detail in this application) Reference Fig.12 The pipetting unit 2 includes a second capping module 21, a pipetting robot 22 and a gun head rack 23. The second capping module 21 is used to realize the opening and closing operations of the sample tube, the purification tube or the finished product tube, and includes a tube body clamping and rotating assembly 132, a tube cover clamping and lifting assembly 131 (the structure is the same as the tube body clamping and rotating assembly 132 and the tube cover clamping and lifting assembly 131 of the first capping module 13, and will not be repeated here) and a second slide rail assembly 211, wherein the second slide rail assembly 211 is used to transport the sample tube, the purification tube or the finished product tube from the extraction unit 1 to the pipetting unit 2 or from the pipetting unit 2 to the extraction unit 1.

[0052] The liquid transfer robot 22 includes a liquid transfer gun and a liquid transfer arm. The liquid transfer arm can drive the mechanical claw to move in the X, Y and Z directions. The liquid transfer gun can be used to extract the liquid in the sample tube and add it to the purification tube, or to extract the liquid in the purification tube and add it to the finished tube or sample bottle. The gun tip rack 23 is used to place the gun tip of the liquid transfer gun.

[0053] In the specific implementation process, the pipetting unit 2 can also be provided with a sample bottle rack 24 for placing sample bottles, wherein the sample bottles are provided with filters and syringe needles in sequence from bottom to top, and the injection needle needle and the gun head connector of the pipetting robot arm 22 are adapted, that is, the gun head connector and the injection needle needle can be quickly plugged and unplugged like the gun head connector and the gun head.

[0054] In order to realize the quick disassembly of the pipette gun tip connector and the gun tip, a gun tip disassembly assembly 25 is also provided, which includes a gun tip recovery cylinder and a gun tip disassembly rack, and a slot for disassembling the gun tip is provided on the top of the gun tip disassembly rack. In order to avoid contamination, the pipette gun needs to replace a new gun tip after each pipetting operation.

[0055] A specific workflow of a fully automatic QuECHERS experimental device in the embodiment of the present application is as follows: 1. At the beginning of the process, the transport robot 12 transports the sample tube to the first capping module 13 to open the cap; during the process of opening the sample tube, the transport robot 12 transports the extraction salt tube to the salt pouring module 15 and opens the extraction salt tube cap; 2. The first slide rail assembly 133 drives the sample tube to move to the bottom of the homogenizer adding module 14 and the salt pouring module 15 in sequence to complete the homogenizer, the extraction liquid and the extraction salt and addition, and then the sample tube returns to the initial position of the first capping module 13 to close the cap; 3. The transport robot 12 transports the capped sample tube to the vortex module 16, the oscillation module 17 and the centrifugal module 18 to complete the corresponding operations, so that the sample is fully mixed and dissolved with the solution and the extraction salt, and then centrifuged and layered; 4. The transport robot 12 transports the purified salt tube and the sample tube that has been vortexed, shaken and centrifuged to the tube body clamping rotating assembly 132 of the second capping module 21, and then the second slide rail assembly 211 drives the tube body clamping rotating assembly 132 to move the extraction unit 1 to the tube cover clamping lifting assembly 131 of the pipetting unit 2 for opening the cover; 5. The liquid transfer robot 22 sucks the supernatant in the sample tube and adds it into the purified salt tube. Then, the second capping module 21 closes the caps on the sample tube and the purified salt tube. The second slide rail assembly 211 transports the sample tube and the purified salt tube from the liquid transfer unit 2 to the extraction unit 1. Then, the transport robot 12 returns the sample tube to the sample tube rack and transports the purified salt tube to the vortex module 16, the oscillation module 17 and the centrifugal module 18 in sequence for corresponding operations. 6. The finished tube and the purified salt tube that has completed the vortex, oscillation and centrifugation operations are transported to the second capping module 21 through the transport robot 12 and the second slide rail assembly 211 for opening the cap. The pipetting robot 22 sucks the supernatant of the purified salt tube and adds it to the finished tube and / or the sample bottle. The pipetting robot 22 removes the gun head (when a sample bottle is provided, after the pipetting robot 22 adds the supernatant of the purified salt tube to the sample bottle, the pipetting robot 22 also needs to remove the gun head and then connect the gun head connector with the syringe needle tube, and pump air inward to allow the supernatant to pass from the syringe needle tube through the filter into the sample bottle) and close the caps of the purified salt tube and the finished tube. Finally, the purified salt tube and the finished tube are returned to the corresponding test tube rack, and one process ends.

[0056] It should be noted that in a specific embodiment, in order to improve experimental efficiency or match relevant experimental requirements, the number of the above-mentioned related modules (including the first tube rack module 11, the first capping module 13, the sample loading module, the vortex module 16, the oscillation module 17 and the centrifugal module 18, the third tube rack module, the second capping module 21, etc.) can be increased or decreased accordingly.

[0057] The above are all preferred embodiments of the present application, and the protection scope of the present application is not limited thereto. Therefore, any equivalent changes made according to the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A fully automatic QuECHERS experimental device, characterized in that: It comprises an extraction unit (1) and a liquid transfer unit (2); The extraction unit (1) comprises: A first tube rack module (11), comprising a plurality of test tube racks for placing test tubes; A transport robot arm (12), comprising a mechanical claw for grabbing the test tube and a transport arm for transporting the test tube to a corresponding workstation; The first capping module (13) comprises a tube cap clamping and lifting assembly (131) for grabbing and lifting the tube cap, a tube body clamping and rotating assembly (132) for clamping and rotating the tube body, and a first slide rail assembly (133) capable of transporting the tube body clamping and rotating assembly (132) to the next workstation; A sample adding module, located at the next station of the first capping module (13), comprising a homogenizer adding module (14) for adding homogenizer and extraction liquid into the test tube and a salt pouring module (15) for adding extraction salt into the test tube; and a vortex module (16), an oscillation module (17), and a centrifugal module (18) for vortexing, oscillating, and centrifuging the sample tube or the purified salt tube; The pipetting unit (2) comprises: A second capping module (21) comprises a tube cap clamping and lifting assembly (131), a tube body clamping and rotating assembly (132), and a second slide rail assembly (211) capable of transporting the tube body clamping and rotating assembly (132) from the extraction unit (1) to the liquid transfer unit (2) or from the liquid transfer unit (2) to the extraction unit (1); A liquid transfer robot (22), used for sucking liquid from a test tube and adding it to another test tube; And a gun tip rack (23) for placing the gun tip of the liquid transfer robot arm (22).

2. A fully automatic QuECHERS experimental device according to claim 1, characterized in that: The pipe cover clamping and lifting assembly (131) comprises: A first clamping claw (1311), used for clamping the tube cover; The clamping cylinder (1312) is used to drive the first clamping jaw (1311) to rise and fall vertically, and a bearing block (1313) is arranged on the top of the clamping cylinder. The first clamping jaw (1311) is vertically slidably connected to the bearing block (1313), and a compression spring (1314) is arranged at the vertical sliding connection.

3. A fully automatic QuECHERS experimental device according to claim 3, characterized in that: The pipe cover clamping and lifting assembly (131) further comprises: The clamping slide rail (1315) is arranged below the clamping cylinder (1312) and is used to drive the clamping cylinder (1312) to slide horizontally.

4. A fully automatic QuECHERS experimental device according to claim 1, characterized in that: The tube body clamping rotating assembly (132) comprises: A sleeve (1321) is disposed on the base plate; A rotating body (1322) is disposed inside the sleeve (1321) and is rotatably connected thereto, and is provided with a lumen for accommodating the tube body; The airbag (1323) is arranged in the tubular cavity and is used to abut against and clamp the tubular body. When the airbag (1323) expands, the airbag (1323) abuts against the tubular body, and the tubular body is clamped and fixed in the tubular cavity; when the airbag (1323) contracts, the airbag (1323) and the tubular body are separated, and the tubular body can be separated from the tubular cavity; And a rotation driving assembly, used for driving the rotating body (1322) to rotate along the sleeve (1321).

5. A fully automatic QuECHERS experimental device according to claim 1, characterized in that: The homogenizer adding module (14) comprises: The sorting cylinder (141) is rotatably connected to the base plate, and a plurality of discharge holes (1411) are evenly arranged at the bottom edge along the circumferential direction thereof; The inclined plate (142) is arranged in the sorting barrel (141), and an arc-shaped groove (1422) is arranged on a lower side along its circumferential direction, and the arc-shaped groove (1422) and the barrel wall of the sorting barrel (141) form a material discharge trough connected to the material discharge hole (1411); A material discharge cylinder (143), which is disposed below the base plate and can be connected to the material discharge hole (1411); And a material discharge driving assembly is used to drive the sorting cylinder (141) to rotate along the base plate, and during the rotation process, the material discharge cylinder (143) can be connected with the material discharge hole (1411) in sequence.

6. A fully automatic QuECHERS experimental device according to claim 5, characterized in that: The homogenizer addition module (14) further comprises a plurality of liquid adding pipes (144), and the plurality of liquid adding pipes (144) are arranged along the circumference of the lower barrel (143).

7. A fully automatic QuECHERS experimental device according to claim 1, characterized in that: The salt pouring module (15) comprises: A balance wheel (151) is rotatably connected to the base plate and is provided with a lever (1511); A pendulum rod (152) is arranged on the other side of the base plate and rotates along with the pendulum wheel (151) in the vertical direction; The second clamping jaw (153) is horizontally arranged at one end of the swing rod (152) away from the balance wheel (151), and is used to clamp the test tube; and when the balance wheel (151) rotates, the second clamping jaw (153) drives the tube body to perform flipping and tilting movements; A connecting plate (154), one end of which is located on a side of the base plate on which the balance wheel (151) is provided and is vertically slidably connected to the base plate, a tension spring (155) is provided at the sliding connection, and when the balance wheel (151) rotates, the lever (1511) can drive the connecting plate (154) to slide vertically downward along the base plate; The other end extends to the other side of the base plate and is vertically provided with a funnel (1541). The funnel (1541) is arranged relative to the second clamping jaw (153). When the balance wheel (151) rotates, the second clamping jaw (153) drives the tube body to flip, and the funnel (1541) moves vertically downward along with the connecting plate (154). When the balance wheel (151) rotates a certain angle, the tube body mouth is inserted into the funnel (1541). And a salt pouring driving assembly, used for driving the balance wheel (151) to rotate along the base plate.

8. A fully automatic QuECHERS experimental device according to claim 7, characterized in that: A vibration motor (156) is provided on the swing rod (152) and / or the connecting plate (154).

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