Method and machine for transplanting fat

Abstract

The present invention relates to a machine (1) for transplanting fat, comprising: - a fat-suction circuit, - a filtration device (100) comprising a filter defining a centrifuging chamber (160) for adipose tissue, the filter having a pore size configured to allow a liquid medium to pass and hold adipose tissue back, - a rotational-drive device (30) connected to the filtration device, - a mass-measurement device (5) configured to measure the mass of adipose tissue present in the filtration device, - a processing unit (10) configured to control the rotational-drive device (300) in such a way as to initiate at least one centrifuging filtration sequence on the basis of a predetermined mass of adipose tissue sucked up, the processing unit also being configured to calculate a quantity (Qinjectable) of adipose tissue that is injectable after each purification sequence.

Description

[0001] Description

[0002] Title of the invention: Fat transplantation method and machine

[0003] Technical Field

[0004] The present invention relates to a method and a machine for fat transplantation operations (referred to as "lipofilling" in English) for aesthetic and reconstructive purposes. The invention finds, in particular, a use for the transplantation of fat useful for breast surgery operations, the implementation of the device which is the subject of the invention not being limited to this application.

[0005] Prior art

[0006] Fat transplantation or autologous adipose tissue grafting operations are used in surgery, particularly in buttock surgery for volume augmentation or in breast surgery to shape the breast to give it a more natural appearance after a DIEP (Deep Inferior Epigastric Perforator), latissimus dorsi flap reconstruction, or after breast implant placement. These operations involve removing fat from a patient's donor area and then reintroducing it into the area of ​​interest on the patient's body, with the removed fat being processed before its reintroduction.

[0007] Regardless of the transplant operation being considered, controlling the amount of fat throughout the transplant process is an important parameter. Indeed, during the liposuction operation, it is important for the practitioner to be able to determine how much fat must be removed to obtain the amount of fat needed for injection. However, the adipose tissue removed is processed to eliminate, in particular, the liquid environment and polluting species (physiological fluid, blood, oil, etc.) present in the tissue. It is therefore understandable that the amount of adipose tissue removed does not correspond to the amount of tissue that can potentially be injected. In the case of breast augmentation or reconstruction, for example, the practitioner determines the volume to be injected during a prior visit in order to obtain an optimal result.Therefore, if the practitioner removes less adipose tissue than is necessary by liposuction, the final result will not be satisfactory. In addition, during the operation of injecting the treated adipose tissue, it is also important for the practitioner to constantly know the quantity of tissue injected and the quantity of tissue still available, particularly for questions of symmetry and / or overall aesthetics, such as in the case of an increase in volume in two separate areas (for example, two breasts or buttocks).

[0008] A technique known from the prior art is to condition the adipose tissue intended for injection in syringes having a determined volume.

[0009] Document US 2020 / 0054824 discloses a closed-loop system in which fat is recovered to be prepared for reintroduction by being mixed with a lavage liquid in a gravity filtration device marketed under the trade name Revolve®, and then reintroduced into the patient's body using a centrifugal pump.

[0010] However, none of the prior art solutions allows the practitioner to precisely assess and monitor the quantity of fat to be removed during liposuction or to easily control in real time the quantity of purified fat injected and remaining.

[0011] Statement of the invention

[0012] For this purpose, the present invention provides a fat transplantation method comprising:

[0013] - the aspiration of adipose tissue into a centrifugation chamber delimited by a filter, the filter having a pore size configured to allow a liquid medium to pass through and retain adipose tissue,

[0014] - filtration by centrifugation of a liquid medium present with the adipose tissue, the centrifugation being carried out continuously or sequentially when a predetermined mass of adipose tissue has been aspirated,

[0015] - the calculation of a quantity of injectable adipose tissue continuously or after each centrifugation sequence based on the mass of adipose tissue present in the centrifugation chamber,

[0016] - purification of adipose tissue comprising mixing a washing liquid with the adipose tissue present in the centrifugation chamber and filtering a fraction of the polluting materials from the adipose tissue by centrifuging the mixture,

[0017] - injection of purified adipose tissue.

[0018] The method of the invention makes it possible to precisely determine by weighing and calculating the quantity of useful adipose tissue, i.e. the quantity of injectable adipose tissue, from the raw adipose tissue removed during aspiration. The practitioner can therefore monitor the evolution of the quantity of injectable adipose tissue during aspiration and stop aspiration at the right time, i.e. when the desired quantity for the treatment has been reached. This ensures that an optimal quantity of fat is removed.

[0019] According to a particular characteristic of the method of the invention, the quantity of injectable adipose tissue (Qinjectabie) is calculated with the following formula:

[0020] Injectable = (a X Qtotal — b) where: a is a correction factor less than 1,

[0021] Qtotaie is the amount of adipose tissue measured after filtration, b is a loss factor.

[0022] Such a formula makes it possible to improve the accuracy of the calculation of the injectable quantity because it takes into account in particular a loss factor linked to the filtration device. According to another particular characteristic of the method of the invention, it further comprises the comparison of the calculated injectable adipose tissue quantity with a target injectable adipose tissue quantity value and a notification step when the calculated injectable adipose tissue quantity has reached the target injectable adipose tissue quantity value. This further facilitates the control of the quantity of adipose tissue to be taken for the practitioner.

[0023] According to another particular characteristic of the method of the invention, it further comprises the determination of the quantity of purified adipose tissue available as a function of the measured mass of purified adipose tissue and a value of lost mass of adipose tissue. This refines the evaluation of the quantity of tissue available before the injection. According to another particular characteristic of the method of the invention, it further comprises the real-time monitoring of the quantity of purified adipose tissue available during the injection of the purified adipose tissue. The practitioner thus knows at all times the quantity of adipose tissue available and can control the treatment very precisely.

[0024] The invention also relates to a fat transplantation machine, the machine comprising at least:

[0025] - a grease suction circuit,

[0026] - a grease injection circuit,

[0027] - a filtration device comprising a filter delimiting a centrifugation chamber for adipose tissue, the filter having a pore size configured to allow a liquid medium to pass through and retain adipose tissue, the filtration device being connected to the fat suction circuit and to the fat injection circuit,

[0028] - a rotary drive device comprising a rotary motor connected to the filtration device,

[0029] - at least one washing liquid tank connected to the filtration device,

[0030] - a mass measuring device configured to measure the mass of adipose tissue present in the filtration device,

[0031] - a processing unit configured to control the rotational drive device so as to initiate at least one centrifugation filtration sequence based on a predetermined mass of aspirated adipose tissue, the processing unit being further configured to calculate a quantity of injectable adipose tissue after each purification sequence.

[0032] The machine according to the invention makes it possible to collect and filter adipose tissue while allowing an accurate assessment of the quantity of adipose tissue actually available for subsequent injection. The fat suction can thus be stopped at the right time, i.e. when it has reached the desired quantity for the treatment, which ensures that an optimal quantity of fat is collected. According to a particular characteristic of the machine according to the invention, the processing unit calculates the quantity of injectable adipose tissue from the following formula: Qinjectable — (a X Qtotal b) where: a is a correction factor less than 1,

[0033] Qtotaie is the amount of adipose tissue measured after filtration, b is a loss factor.

[0034] Such a formula makes it possible to improve the accuracy of the calculation of the injectable quantity because it takes into account in particular a loss factor linked to the filtration device. According to another particular characteristic of the machine according to the invention, the processing unit is further configured to compare the calculated injectable adipose tissue quantity with a target injectable adipose tissue quantity value and to issue a notification when the calculated injectable adipose tissue quantity has reached the target injectable adipose tissue quantity value. This further facilitates the control of the quantity of adipose tissue to be collected.

[0035] According to another particular characteristic of the machine according to the invention, the processing unit is further configured to control the suction of the washing liquid into the filtration device and to control the rotational drive device so as to initiate at least one purification sequence by filtration of a fraction of the polluting materials from the adipose tissue, the processing unit being further configured to determine the quantity of purified adipose tissue available as a function of the measured mass of purified adipose tissue and a value of lost mass of adipose tissue. The evaluation of the quantity of tissue available before injection can thus be further refined.

[0036] According to another particular characteristic of the machine according to the invention, the processing unit is configured to monitor in real time the quantity of purified adipose tissue available during the injection of the purified adipose tissue.

[0037] The evolution of the quantity of available adipose tissue can thus be controlled very precisely throughout the injection stage.

[0038] Brief description of the drawings

[0039] [Fig. 1] Figure 1 is a schematic perspective view of a fat transplantation machine in accordance with one embodiment of the invention. [Fig. 2] Figure 2 is an exploded schematic view of a filtration device of the machine of Figure 1, [Fig. 3] Figure 3 is a schematic sectional view of a filtration device.

[0040] Description of the embodiments

[0041] Figure 1 illustrates an example of a fat transplantation machine 1 according to the invention. In general and as will be detailed further below, the machine 1 comprises:

[0042] - a filtration device 100,

[0043] - a rotation drive device 30 connected to the filtration device,

[0044] - a grease suction circuit comprising a suction cannula 50 and pipes 175, 177 and 171 connecting the suction cannula to the filtration device 100,

[0045] - a grease injection circuit comprising an injection cannula 55 and pipes 179, 178 and 171 connecting the cannula to the filtration device 100,

[0046] - a tank 70 containing a washing liquid 71 connected to the filtration device 100 by pipes 182, 181, 180, 178 and 171,

[0047] - a reservoir 80 containing an infiltration liquid 81 connected to the injection cannula 55 by pipes 183, 181, 180 and 179,

[0048] - a mass measuring device 5 connected here to the assembly comprising a part of the rotation drive device 30 and the filtration device 100,

[0049] - a circulation pump 20,

[0050] - a 60 suction pump,

[0051] - a pinch or throttle valve device 40,

[0052] - a processing unit 10 allowing the implementation of the different stages of fat transplantation according to the invention, the processing unit 10 being connected to the rotation drive device 30, to the mass measuring device, to the circulation pump 20, to the suction pump 60 and to the pinch valve device 40.

[0053] As illustrated in Figures 2 and 3, the filtration device 100 comprises a sealed enclosure 110 formed here by a cover 111, a cylindrical wall 112 and a bottom 113. These elements are fixed together in a sealed manner.

[0054] The purification device 100 also comprises a filter or screen 120 present in the sealed enclosure 110. The filter 120 delimits a centrifugation chamber 160 (FIG. 3) whose operation is described later. In the example described here, the filter 120 has a cylindrical shape. The filter may have other shapes suitable for centrifugation. The filter 120 has a pore size configured to allow a liquid medium to pass through and retain adipose tissue. The pore size is chosen in particular to allow the passage of liquids such as oil, blood, water or a physiological solution while retaining the adipose tissue. As non-limiting examples, the pore size of the filter may be between 50 μm and 1500 μm and more preferably between 200 μm and 500 μm.

[0055] In the example described here, a stiffening element 130 of cylindrical shape is present between the cylindrical wall 112 of the sealed enclosure 110 and the filter 120, the stiffening element 130 being for example made of metallic or plastic material and having an openwork structure defining a plurality of openings 1300 in order to allow the evacuation of the liquid medium drained by the filter 120.

[0056] The stiffening element 130 is associated with a rotary plate 131. More precisely, the stiffening element 130 comprises at its lower end teeth 1301 which cooperate with grooves 1310 present in the vicinity of the external periphery of the rotary plate 131. The rotary plate 131 is connected to the rotational drive device 30. The rotational drive device comprises a rotary electric motor 300. The electric motor 300 may be, for example, a stepper motor or a brushless direct current motor. In the example described here, the rotary plate 131 is connected to the rotary electric motor 300 by a bidirectional clutch 350 configured to ensure, via a first shaft 351, the rotational drive of the rotary plate 131 in a first direction of rotation Si of the motor 30 (FIG. 3). The bidirectional clutch may be replaced by any device allowing selective rotational drive in two opposite directions of rotation.

[0057] Centrifugation is performed by rotating the filter 120. More specifically, the electric motor 300 is controlled in a first direction of rotation Si to drive the rotating plate 131 and the stiffening element 130 in engagement with the plate 131. The rotation of the plate 131 and the stiffening element 130 causes the filter 120 to rotate. The filter is attached to the stiffening element by any suitable means. The speed of the electric motor is controlled so as to apply centrifugal acceleration to the adipose tissue present in the centrifugation chamber. Thus, adipose tissue present in the centrifugation chamber 160 will be subjected to a centrifugal force against the inner wall of the filter 120, which makes it possible to effectively drain the liquid medium present in the adipose tissue without damaging it.

[0058] During centrifugation, the liquid medium passing through the filter 120 and the stiffening element 130 is collected in a volume 165 delimited between the stiffening element 130 and the cylindrical wall 112 of the enclosure 110. The liquid is then evacuated via an evacuation port 1131 present on the bottom 113 of the enclosure 110 connected to a pipe 173.

[0059] In the example described here, the cover 111 comprises three ports 1110, 1111 and 1112 intended to be connected to a venting device 90 provided with a filter 91, to the grease injection circuit and to the reservoir 70 containing the washing liquid 71 via pipes 170, 171, 172.

[0060] The purification device comprises a cover 101 having openings 1010, 1011 and 1012 which cooperate with the ports 1110, 1111 and 1112 of the hood 111.

[0061] In the example described here, the purification device further comprises a collection tray 140 which is movable in translation in a direction D Talong the axis of the filter 120. More specifically, the purification device 100 comprises a threaded rod 141 which extends vertically inside the centrifugation chamber 160 along the axis of the filter 120. The lower end 1412 of the threaded rod is connected to the electric motor 300 via a guide 150 and the bidirectional clutch 350. The guide 150 comprises a housing 1500 in which the lower end 1412 is fixed. A lower portion 1501 of the guide 150 is connected to a part of the bidirectional clutch 350 which is engaged with the electric motor 300 only when the latter transmits a rotational movement in a second direction of rotation S2 opposite to the first direction of rotation Si used for centrifugation. When the electric motor 300 rotates in the first direction of rotation Si, no rotational movement is transmitted by the bidirectional clutch 350 to the guide 150 to which the threaded rod 141 is connected.Similarly, when the motor 300 rotates in the second direction of rotation S2, no rotational movement is transmitted by the bidirectional clutch 350 to the rotary plate 131 and, consequently, to the filter 120. The collection plate 140 has on its upper face a central opening 1400 extended by a neck 1401 which extends from the lower face of the plate 140. The neck 1401 comprises a portion 1402 comprising a tapping 1403 which cooperates with a thread 1411 of the threaded rod 141. Thus, when the threaded rod is rotated in the second direction of rotation S2, the collection plate 140 rises in the centrifugation chamber 160 in the direction DT and acts as a piston.

[0062] In another embodiment, the filter is made of a self-supporting rigid material. The filter may in particular be made from a metal strip in which holes are made by laser, water jet or chemical cutting in order to form a screen, a metal grid woven from wires or balls agglomerated by sintering metal or ceramic powders. In this case, the stiffening element 130 is no longer necessary and it is the self-supporting filter which is directly engaged with the rotating plate 131.

[0063] In the example described here, the mass measuring device 5 corresponds to a strain gauge 51 interposed between a support plate 52 on which rests the assembly comprising the bidirectional clutch 350 of the rotation drive device 30 and the filtration device 100 and a base plate 53 secured to the frame of the machine 1. Spacers 54 are also interposed between the support plate 52 and the base plate 53, these spacers acting as safety stops in order to protect the strain gauge 51 from untimely movements and / or overloads of the filtration device in a vertical and horizontal direction. Any other mass measuring device making it possible to measure the mass of the filtration device 100 or of the assembly formed by the rotation drive device 30 and the filtration device 100 may be used.The strain gauge 51 comprises a signal conditioner (not shown in FIGS. 1 and 2) which transforms an electric current representative of the mass measurement into a digital signal corresponding to the measured mass which is transmitted to the processing unit 10. The pinch valve device 40 comprises a plurality of pinch modules 41 to 48 also called "pinch valves" capable of selectively pinching one or more pipes in order to prevent circulation and of releasing the pinch in order to authorize this circulation according to the commands received from the processing unit 10.

[0064] The processing unit 10 here has the hardware architecture of a computer. It comprises in particular a processor, a random access memory, a read only memory and a non-volatile flash memory. The processing unit 10 also comprises means of communication with the rotation drive device 30, the mass measuring device 5, the circulation pump 20, the suction pump 60 and the pinch valve device 40. The communication means integrate for example digital data buses known per se, allowing various electronic entities to communicate with each other. The processing unit 10 further comprises a display screen 11.

[0065] The various steps of a fat transplantation method with the machine 1 are now described. Before each transplantation method is carried out, a preferably single-use filtration device 100 is connected to the rotary drive device 30 of the machine 1. A tare is then carried out in order to deduce the mass of the part of the machine which rests on the weighing member, namely in the example described here the mass of the filtration device 100 and the bidirectional clutch 350. This tare makes it possible to initialize the mass measurement to zero in order to subsequently measure only the mass of adipose tissue present in the filtration device. The tare can be carried out automatically by programming the processing unit or by the practitioner, for example via a touch button on the display screen 11.

[0066] A first step which may be optional consists of injecting an infiltration liquid 81 containing physiological liquid with possibly an anesthetic into the area of ​​the patient's body where adipose tissue is to be removed (infiltration). In this infiltration step, the processing unit 10 places the pinching modules 45 and 47 in the open position allowing circulation in the pipes 179 and 183 (the other pinching modules being placed in the closed position) and controls the actuation of the pump 20 so that the infiltration liquid 81 present in the reservoir 80 is sucked up by the pipe 183. The liquid is conveyed into the pump 20 by the pipe 181 and is then discharged from it by the pipe 180 and the pipe 179 in order to reach the injection cannula 55 used to introduce the infiltration liquid into the patient's body.

[0067] The next step is to aspirate adipose tissue for subsequent reimplantation. In this adipose tissue removal step, the processing unit 10 places the pinching modules 42 and 43 in the open position allowing circulation in the pipes 177 and 174 (the other pinching modules being placed in the closed position). The actuation of the suction pump 60 by the processing unit 10 makes it possible to draw a vacuum into the filtration device 100 via the pipes 176, 174, 173 and 172, which causes the adipose tissue to be sucked up by the suction cannula 50 and conveyed into the filtration device 100 via the pipes 175, 177 and 171.

[0068] The liquid medium present in the aspirated adipose tissue and mainly consisting of oil, blood and possibly infiltration liquid is filtered by centrifugation during the aspiration of the adipose tissue. The centrifugation can be carried out continuously or sequentially. In the latter case, centrifugation sequences are programmed in the processing unit 10 according to the mass of adipose tissue present in the centrifugation chamber 160 of the filtration device 100. More precisely, the processing unit 10 is programmed to initiate a centrifugation sequence as soon as a determined mass of adipose tissue has been aspirated into the filtration device, the processing unit receiving in real time a measurement of the mass of the adipose tissue present in the filtration device. As non-limiting examples, a centrifugation sequence can be initiated every 50, 100 or 150 grams of adipose tissue aspirated.

[0069] Continuously or after each centrifugation sequence, the processing unit calculates the quantity of injectable adipose tissue based on the mass of adipose tissue present in the centrifugation chamber.

[0070] The mass of injectable adipose tissue can be calculated with the following formula: Qinjectable = (a X Qtotal — b) where: • a is a correction factor less than 1,

[0071] • Qtotaie is the quantity of adipose tissue measured after filtration,

[0072] • b is a loss factor.

[0073] The factor a can be determined by tests in which the quantity of tissue weighed by the machine after filtration (Qtotaie) is compared with the quantity of tissue actually recovered after completely emptying the centrifugation chamber. The value of the quantity Qtotaie corresponds to the mass of adipose tissue measured by the mass measuring device 5 converted into volume, considering for example that a mass of 1 gram of adipose tissue corresponds to a volume of 1 milliliter of adipose tissue.

[0074] The factor b corresponds to a fraction of liquid stagnating in the filtration device (i.e. not evacuated) and to a fraction of adipose tissue attached to the filter and which cannot be reinjected. The fraction of stagnant liquid is constant while the fraction of adipose tissue blocked in the filter depends on the quantity of adipose tissue centrifuged. Values ​​of the factor b can be determined by tests or by simulation. In the case of tests, a determined gross mass of adipose tissue is centrifuged. The accessible quantity of adipose tissue is extracted from the filtration device. The mass of this extracted quantity is measured and subtracted from the determined gross mass in order to obtain a value of lost mass. The conversion into volume of the lost mass then makes it possible to define the value of the loss factor b. It is thus possible to extrapolate a chart from several values ​​of the loss factor obtained by tests.

[0075] Below is a non-limiting example of a calculation when filtration is carried out by centrifugation after having aspirated 100 g of adipose tissue, 100 g corresponding here to the frequency of the centrifugation sequences:

[0076] The measured mass of adipose tissue after centrifugation (elimination of the liquid phase present in the tissue) is 85 g, i.e. a total quantity Q of 85 ml. The value of the correction factor a is set at 0.8 and the value of the loss factor b is defined at 40 ml.

[0077] The quantity of injectable tissue Qinjectabie is therefore: (0.8 x 85) - 40 = 28 ml. The aspiration of the adipose tissue ends when the calculated quantity of injectable tissue corresponds to that which the practitioner needs for the injection. The practitioner can himself follow on the display screen 11 the evolution of the result of the calculation of the quantity of injectable tissue and stop the aspiration when this quantity is suitable for him. According to a particular aspect of the invention, the processing unit 10 can be programmed to signal to the practitioner when the desired quantity of injectable tissue is reached. For this purpose, the practitioner enters a value for the target quantity of injectable adipose tissue, for example using touch keys on the display screen 11. During the aspiration of the adipose tissue, the processing unit 10 compares the calculated quantity of injectable adipose tissue with the value for the target quantity of injectable adipose tissue.Once the target quantity is reached, the treatment unit notifies the practitioner, for example by an audible signal and / or information displayed on the display screen.

[0078] When the calculated quantity of injectable tissue corresponds to the desired quantity, the suction is stopped. The adipose tissue is then purified by washing and centrifugation. For this purpose, the processing unit 10 places the pinching modules 44 and 46 in the open position allowing circulation in the pipes 178 and 179 (the other pinching modules being placed in the closed position). By actuating the pump 20 by the treatment unit 10, a washing liquid 71 present in the reservoir 70 is sucked in by the pipe 182. The liquid is conveyed into the pump 20 by the pipe 181 and then is discharged from the latter by the pipe 180, the pipe 178 and the pipe 171 in order to reach the filtration device 100. During the introduction of the washing liquid into the filtration device 100, one or more centrifugation cycles are carried out as described above to filter the polluting materials.The evacuation of the washing liquid containing the polluting materials (physiological liquid, blood, oil, etc.) from the adipose tissue is carried out by actuating the suction pump 60 and placing the pinching modules 41, 43 and 48 in the open position by the processing unit 10. In this configuration, the washing liquid containing the polluting materials is sucked through the discharge port 1131 of the filtration device 100 and then circulates in the pipes 173, 174 and 176 in order to be conveyed into a bin 61 present upstream of the suction pump 60. The placement of the module 48 in the open position allows the filtration device to be ventilated and thus facilitates the evacuation of the washing liquid containing the polluting materials. The purification of the adipose tissue preferably comprises two successive cycles, each comprising bringing the adipose tissue into contact with the washing liquid and centrifuging the mixture.Once the adipose tissue has been purified, the processing unit determines the quantity of purified tissue available for injection. This determination simply consists of measuring the mass of purified adipose tissue present in the filtration device 100, converting it into volume (quantity) and subtracting from it the loss factor b defined for the quantity of tissue in question. This gives an accurate value of the quantity of adipose tissue available for injection. This value is displayed on the screen 11.

[0079] The practitioner then proceeds to inject the adipose tissue. The treatment unit 10 places the pinch modules 44 and 45 in the open position allowing the circulation of the adipose tissue in the pipes 171, 178 and 179 (the other pinch modules being in the closed position). The purified adipose tissue can thus be conveyed from the centrifugation chamber to the injection cannula 55. The collection tray 140 can be used as a piston which pushes the purified adipose tissue towards the upper part of the centrifugation chamber as close as possible to the port 1111. A pump can also be interposed in the injection circuit to assist in conveying the adipose tissue to the injection cannula 55.

[0080] Throughout the injection, the mass of the adipose tissue present in the centrifugation chamber is transmitted to the processing unit 10. The processing unit calculates in real time the quantity still available for injection by comparison with the initial value of the quantity of adipose tissue available. The quantity of adipose tissue injected and / or the quantity of adipose tissue still available is indicated on the display screen 11.

Claims

Demands

1. A grease treatment process comprising: - the filtration by centrifugation of a liquid medium present with adipose tissue in a centrifugation chamber (160) delimited by a filter (120), the filter having a pore size configured to allow a liquid medium to pass through and retain adipose tissue, the centrifugation being carried out when a predetermined mass of adipose tissue is present in the centrifugation chamber, - the calculation of a quantity of injectable adipose tissue (Qinjectabie) as a function of the mass of adipose tissue present in the centrifugation chamber (160), - the purification of adipose tissue comprising mixing a washing liquid (71) with the adipose tissue present in the centrifugation chamber and filtering a fraction of the polluting materials from the adipose tissue by centrifuging the mixture.

2. A method according to claim 1, wherein the quantity of injectable adipose tissue (Qinjectabie) is calculated using the following formula: Qinjectabie = (a X Qtotal — b) where: a is a correction factor less than 1, Qtotaie is the amount of adipose tissue measured after filtration, b is a loss factor.

3. A method according to claim 1 or 2, further comprising comparing the calculated quantity of injectable adipose tissue (Qinjectabie) to a target quantity of injectable adipose tissue value and a notification step when the calculated quantity of injectable adipose tissue has reached the target quantity of injectable adipose tissue value.

4. A method according to any one of claims 1 to 3, further comprising determining the quantity of purified adipose tissue available as a function of the mass of purified adipose tissue measured and a value of lost mass of adipose tissue.

5. A method according to claim 4, further comprising real-time monitoring of the amount of purified adipose tissue available.

6. Fat transplantation machine (1), the machine comprising at least: - a grease suction circuit, - a grease injection circuit, - a filtration device (100) comprising a filter delimiting a centrifugation chamber (160) for adipose tissue, the filter having a pore size configured to allow a liquid medium to pass through and retain adipose tissue, the filtration device being connected to the fat aspiration circuit and the fat injection circuit, - a rotary drive device (30) comprising a rotary motor (300) connected to the filtration device, - at least one reservoir (70) of washing liquid (71) connected to the filtration device (100), - a mass measurement device (5) configured to measure the mass of adipose tissue present in the filtration device, - a processing unit (10) configured to control the rotating drive device (300) so as to initiate at least one centrifugal filtration sequence based on a predetermined mass of aspirated adipose tissue, the processing unit being further configured to calculate an amount of injectable adipose tissue (Qinjectabie) after each purification sequence.

7. Machine according to claim 6, wherein the processing unit (10) calculates the quantity of injectable adipose tissue from the following formula: Qinjectabie = (a X Qtotal — b) where: a is a correction factor less than 1, Qtotaie is the amount of adipose tissue measured after filtration, b is a loss factor.

8. Machine according to claim 6 or 7, wherein the processing unit (10) is further configured to compare the calculated amount of injectable adipose tissue to a target amount of injectable adipose tissue value and to issue a notification when the calculated amount of injectable adipose tissue has reached the target amount of injectable adipose tissue value.

9. Machine according to any one of claims 6 to 8, wherein the processing unit (10) is further configured to control the aspiration of a washing liquid (71) into the filtration device (100) and to control the rotating drive device (300) so as to initiate at least one purification sequence by filtration of a fraction of the polluting materials from the adipose tissue, the processing unit being further configured to determine the quantity of purified adipose tissue available as a function of the mass of purified adipose tissue measured and a value of lost mass of adipose tissue.

10. Machine according to claim 9, wherein the processing unit (10) is configured to track in real time the amount of purified adipose tissue available during the injection of the purified adipose tissue.

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