Cell centrifugation device, method, controller and medium

Abstract

The invention provides a cell centrifugal device and method, a controller and a medium. The cell centrifugal device comprises a cup body provided with a centrifugal space and a first channel, the cup body is provided with a rotating axis and can rotate around the rotating axis; the first channel comprises a conveying channel arranged at the top of the cup body and a curve arranged on the side wall of the cup body; a first opening and a second opening are respectively formed in two ends of the curve; the first opening is formed in the inner side wall of the cup body; the bend communicates with the centrifugal space through the first opening and communicates with the conveying channel through the second opening. The bend further comprises at least one bending channel located on the outer side of the inner side wall of the cup body. Through the design of the curve and the conveying channel on the cup body, the minimum concentration volume is reduced, the cost is reduced, and the cell centrifugation efficiency is improved.

Description

Technical Field

[0001] The present invention belongs to the technical field of cell centrifugation, and in particular relates to a cell centrifugation device, method, controller and medium. Background Art

[0002] At present, when performing cell separation, it is often necessary to use a cylindrical centrifugal device to perform cell centrifugation operations, using the principle that the centrifugal force increases radially in a circle to separate different components in the cell fluid. However, in the process of centrifuging the target cells by the centrifugal device in the prior art, there is generally a problem of large residual volume when extracting impurities and culture medium other than the target cells, which will result in a large minimum concentrated volume that cannot meet the requirements of pharmaceutical companies. Therefore, it is necessary to connect different equipment for multiple centrifugation operations to meet the requirements, which is inefficient and costly. At the same time, the centrifugal device in the prior art also has problems such as slow processing speed and long processing time. It will also lead to low processing efficiency and increased costs. Summary of the invention

[0003] The present invention aims at the technical problems of large residual volume and low efficiency when a cell centrifuge is used for centrifugal operation in the prior art, and provides a cell centrifuge device, method, controller and medium.

[0004] In view of the above technical problems, an embodiment of the present invention provides a cell centrifuge device, comprising a cup body provided with a centrifugal space and a first channel; the cup body has a rotation axis, and the cup body can rotate around the rotation axis;

[0005] The first channel includes a conveying channel arranged at the top of the cup body, and a bend arranged on the side wall of the cup body; a first opening and a second opening are respectively provided at both ends of the bend; the first opening is arranged on the inner wall of the cup body; the bend is connected to the centrifugal space through the first opening, and the bend is connected to the conveying channel through the second opening; the bend also includes at least one bending channel located outside the inner wall of the cup body.

[0006] A cell centrifugation method, the cell centrifugation method is performed by the cell centrifugation device, the cell centrifugation method comprises:

[0007] Controlling a first preset dose of separation liquid to flow into the centrifugal space in the cup body of the cell centrifuge device;

[0008] After controlling the cup body to rotate around the rotation axis at a first preset speed, controlling a second preset dose of sample liquid to flow into the centrifugal space at a preset flow rate;

[0009] Controlling the cup body to rotate at a second preset speed for a first preset time to centrifuge the liquid in the centrifugal space in the cup body, so that the target cells in the sample liquid are closely attached to the inner wall of the cup body;

[0010] After the centrifugal operation, the liquid except the target cells is discharged from the centrifugal space through the first opening of the first channel, the bend and the transport channel in sequence.

[0011] Optionally, after controlling the second preset dose of sample liquid to flow into the centrifugal space at a preset flow rate, the method further comprises:

[0012] A third preset dose of separation liquid is controlled to flow into the centrifugal space.

[0013] Optionally, the cup body further comprises a second channel, which is arranged at the bottom of the cup body and communicates with the centrifugal space; after the liquid except the target cells after the centrifugal operation is discharged from the centrifugal space through the first opening, the bend and the delivery channel in sequence, the method further comprises:

[0014] When it is detected that the liquid discharged from the first channel has been exhausted, controlling the target base liquid to flow into the centrifugal space;

[0015] Controlling the cup body to rotate at a third preset speed for a second preset time to evenly mix the target cells in the centrifugal space with the target base liquid to obtain a mixed liquid;

[0016] The mixed liquid in the centrifugal space is discharged into a preset storage container through the second channel.

[0017] A controller comprises a processor and a memory, wherein the memory stores an executable program, and the processor is used to execute the executable program to implement the cell centrifugation method.

[0018] A computer-readable storage medium stores a computer program, wherein the computer program implements the cell centrifugation method when executed by a processor.

[0019] The cell centrifuge device provided by the present invention comprises a cup body provided with a centrifugal space and a first channel; the cup body has a rotation axis, and the cup body can rotate around the rotation axis; the first channel comprises a conveying channel arranged at the top of the cup body, and a bend arranged on the side wall of the cup body; a first opening and a second opening are respectively arranged at both ends of the bend; the first opening is arranged on the inner side wall of the cup body; the bend is connected to the centrifugal space through the first opening, and the bend is connected to the conveying channel through the second opening; the bend also comprises at least one bending channel located outside the inner side wall of the cup body. The present invention can maximize the extraction of other liquids except the target cells attached to the inner side wall of the cup body while avoiding the extraction of target cells as much as possible through the design of the bend and the conveying channel on the cup body, thereby minimizing the liquid residue, reducing the minimum concentration volume, reducing the cost, and improving the cell centrifugation efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention is further described below in conjunction with the accompanying drawings and embodiments.

[0021] Figure 1 It is a schematic diagram of the structure of a cell centrifuge device provided in one embodiment of the present invention.

[0022] Figure 2 It is a schematic structural diagram of a cup cover provided by another embodiment of the present invention.

[0023] Figure 3 It is a schematic structural diagram of a liquid pusher plate provided in one embodiment of the present invention.

[0024] Figure 4 It is a schematic diagram of the process of the cell centrifugation method provided in one embodiment of the present invention.

[0025] Figure 5 It is a schematic diagram of the process of the cell centrifugation method provided in another embodiment of the present invention.

[0026] Figure 6 It is a schematic diagram of the process of the cell centrifugation method provided in another embodiment of the present invention.

[0027] The reference numerals in the specification are as follows:

[0028] 100, cup body; 110, centrifugal space; 120, first channel; 121, conveying channel; 122, bend; 1221, first opening; 1222, second opening; 1223, bending channel; 130, groove; 140, conveying groove; 150, winding groove; 160, second channel; 161, third opening; 171, straight wall section; 172, slope section; 180, cup body; 190, cup cover; 191, first inlet and outlet channel; 192, second inlet and outlet channel; 200, liquid pushing plate; 210, liquid pushing body; 220, guide part; 211, connecting part; 212, pushing part. DETAILED DESCRIPTION

[0029] In order to make the technical problems, technical solutions and beneficial effects solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0030] It should be understood that the directions or positional relationships indicated by terms such as "upper", "lower", "left", "right", "front", "back", and "middle" are based on the directions or positional relationships shown in the accompanying drawings and are only for the convenience of describing the present invention and simplifying the description. They do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as a limitation of the present invention.

[0031] like Figures 1 to 3 As shown, an embodiment of the present invention provides a cell centrifuge device, including a cup body 100 provided with a centrifugal space 110 and a first channel 120; the cup body 100 has a rotation axis, and the cup body 100 can rotate around the rotation axis; the first channel 120 includes a conveying channel 121 arranged at the top of the cup body 100, and a bend 122 arranged on the side wall of the cup body 100; the two ends of the bend 122 are respectively provided with a first opening 1221 and a second opening 1222; the first opening 1221 is arranged on the inner wall of the cup body 100; the bend 122 is connected to the centrifugal space 110 through the first opening 1221, and the bend 122 is connected to the conveying channel 121 through the second opening 1222; the bend 122 also includes at least one bending channel 1223 located outside the inner wall of the cup body 100. It can be understood that in Figure 1 In the embodiment shown, the second opening 1222 is located at a position substantially flush with the first opening 1221 (i.e., the inner side wall of the cup body 100), but in other embodiments, the second opening 1222 can also be located on the outer side or the inner side of the inner side wall of the cup body 100, and is not limited thereto. The cup body 100 can be cylindrical or a column similar to a cylindrical shape.

[0032] Furthermore, the cell centrifuge device further comprises a rotation drive mechanism (not shown) connected to the cup body 100. The cup body 100 is driven by the rotation drive mechanism to rotate around the rotation axis to centrifuge the liquid contained in the centrifugal space.

[0033] In one embodiment, the first distance between the inflection point of the bending channel 1223 and the rotation axis is greater than the second distance between the inner side wall of the cup body 100 and the rotation axis. The bending channel 122 may be composed of one or more bending channels 1223 connected in sequence, and the inflection point refers to the point where each bending channel 1223 turns, and the inflection point includes an outer inflection point and an inner inflection point; for example, Figure 1 In the embodiment shown, the inflection point includes an outer inflection point located at the leftmost side of the bending channel 1223, and also includes an inner inflection point located at a position corresponding to the outer inflection point (i.e. Figure 1 In this embodiment, within the preset height range where the bend 122 is provided on the inner wall of the cup body 100, the distance between any point on the inner wall of the cup body 100 and the rotation axis is the second distance, and all the second distances are smaller than the first distance between the inflection point of the bend channel 1223 and the rotation axis, so that the bend channel 1223 can always be located outside the entire first opening 1222 away from the rotation axis (for example, Figure 1 Only one bending channel 1223 is provided, and the bending channel 1223 is located outside the first opening 1222 away from the rotation axis).

[0034] Furthermore, the bending channel 1223 includes but is not limited to a U-shaped bending channel or a J-shaped bending channel. Figure 1 In the cell centrifuge device shown in the figure, only one bending channel 1223 is provided. When the second opening 1222 is located near the inflection point, the bending channel 1223 is a J-shaped bending channel; when the second opening 1222 is located at a position substantially flush with the first opening 1221 (i.e., the inner wall of the cup body 100), the bending channel 1223 is a U-shaped bending channel.

[0035] In this embodiment, one end of the delivery channel 121 away from the bend 122 is connected to the outside, so that fluid (for example, fluid such as cell fluid, separation fluid or gas) can be input or output from the centrifugal space 110 of the cup body 100 through the delivery channel 121 and the bend 122. The inner wall of the cup body 100 refers to the side wall of the side wall of the cup body 100 close to the rotation axis, that is, the inner wall of the centrifugal space 110. The cup body 100 of the cell centrifuge device is in a state of rotating around the rotation axis during the centrifugation process. Therefore, the target cells (target cells are generally relatively heavy) in the centrifugal space 110 are attached to the inner wall of the cup body 100 under the action of centrifugal force. The bending channel 1223 is located on the outer side of the inner wall of the cup body 100, that is, the bending channel 1223 is bent in a direction away from the inner wall of the cup body 100, and the bending channel 1223 is located between the inner wall and the outer wall of the cup body 100; and the first opening 1221 is located on the inner wall, such as Figure 1 As shown, the bend 122 only includes one bending channel 1223, and the only bending channel 1223 is connected to the first opening 1221. In this way, when the liquid in the centrifugal space 110 is extracted through the first channel 120, the liquid will enter the bending channel 1223 from the first opening 1221. Since the bending channel 1223 is bent in the direction away from the inner wall of the cup body 100, the flow direction of the fluid entering the bending channel 1223 from the first opening 1221 will be from the inner wall of the cup body 100 toward the outer wall (basically no or very little thrust from bottom to top is generated). Therefore, in the entire process of extracting liquid through the first channel 120, since the target cells have been pressed and attached to the inner wall of the cup body 100 under the action of centrifugal force, and there is basically no thrust from bottom to top, extracting liquid through the first channel 120 will minimize the target cells from being extracted synchronously.

[0036] At the same time, when the liquid in the centrifugal space 110 is extracted through the first channel 120, since the cup body 100 of the cell centrifuge device is in a state of rotating around the rotation axis during the centrifugation process, the liquid in the centrifugal space 110 other than the target cells will also adhere to the inner wall of the cup body 100 under the action of centrifugal force. Therefore, the first opening 1221 is set on the inner wall of the cup body 100, which can maximize the extraction of liquid other than the target cells that are not attached to the inner wall of the cup body 100, thereby minimizing liquid residue; compared with the top channel solution in which the channel opening is set at the top, when the liquid in the centrifugal space is extracted through the top channel, more liquid will inevitably remain at the end of the extraction (when the liquid is extracted to a position close to the inner wall of the cup body, a part of the liquid will inevitably be unable to be extracted due to the presence of gas in the channel, or gas will be extracted along with the liquid).

[0037] In one embodiment, the first opening 1221 is disposed at the upper portion of the inner side wall of the cup body 100. It can be understood that the delivery channel 121 is disposed at the top of the cup body 100, and the bend 122 is connected to the delivery channel 121 through the second opening 1222. Therefore, the first opening 1221 of the bend 122 is disposed at the upper portion of the inner side wall of the cup body 100, so that the bend 122 can be kept relatively short in length, thereby reducing the amount of liquid residue in the bend 122 and reducing the manufacturing cost of the bend 122; and the first opening 1221 is disposed at the upper portion of the inner side wall of the cup body 100, so that the situation that more target cells flow into the first opening 1211 due to the sliding of some target cells under the influence of their own gravity can be avoided.

[0038] The cell centrifuge device provided by the present invention includes a bend 122 arranged on the side wall of the cup body 100, the first opening 1221 of the bend 122 is arranged on the inner wall of the cup body 100, and the bending channel 1223 of the bend 122 is located on the outer side of the inner wall of the cup body 100. The present invention can maximize the extraction of other liquids except the target cells attached to the inner wall of the cup body 100 while avoiding the extraction of target cells as much as possible through the design of the bend 122 and the conveying channel 121 on the cup body 100, thereby minimizing the liquid residue, reducing the minimum concentration volume, reducing the cost, and improving the cell centrifugation efficiency.

[0039] like Figure 1 and Figure 3 As shown, in some embodiments, the cell centrifuge device further includes a liquid pusher plate 200 mounted on the cup body 100 and located in the centrifugal space 110; when the cup body 100 rotates around the rotation axis, the liquid pusher plate 200 rotates synchronously with the cup body 100, thereby pushing the liquid to rotate synchronously in the same rotation direction as the cup body 100. It can be understood that when the cup body 100 rotates, the liquid pusher plate 200 rotates synchronously with the cup body 100, thereby reducing the relative movement between the outer layer liquid close to the inner side wall of the cup body 100 and the inner layer liquid close to the rotation axis. The power source for the rotation of the inner layer liquid is no longer the viscous resistance between the outer layer liquid and the inner layer liquid, but the thrust of the liquid pusher plate 200, thereby avoiding the shearing effect of the target cells in the liquid when the viscous resistance acts on the target cells, and avoiding the shear damage to the target cells during the centrifugation process. It can be understood that the specific number of the liquid pusher plates 2 can be 1, 2, 3 or 4, etc., which is not specifically limited here.

[0040] like Figures 1 to 3As shown, in some embodiments, the top of the cup body 100 is provided with a conveying groove 140, and the side wall of the cup body 100 is provided with a rewinding groove 150 connected to the conveying groove 140; the liquid pushing plate 200 includes a liquid pushing body 210 and a guide portion 220 connected to the side of the liquid pushing body 210 away from the rotation axis; the top end of the liquid pushing body 210 is inserted into the conveying groove 140 and surrounds the conveying channel 121 with the groove wall of the conveying groove 140, and the bottom end of the liquid pushing body 210 is connected to the bottom surface of the cup body 100; the guide portion 220 is inserted into the rewinding groove 150 and surrounds the bend 122 with the rewinding groove 150. It can be understood that the conveying channel 121 can be generated by directly processing the top of the cup body 100, or by enclosing multiple parts; the bend 122 can be generated by directly processing the side wall of the cup body 100, or by enclosing multiple parts. In this embodiment, in order to reduce the difficulty of processing, the delivery channel 121 is formed by the top end of the liquid pushing body 210 inserted into the delivery groove 140 and surrounded by the groove wall of the delivery groove 140, and the bend 122 is formed by the guide part 220 inserted into the rewinding groove 150 and surrounded by the rewinding groove 150. In this way, the liquid pushing plate 210 is fully utilized, and the components are reduced while reducing the difficulty of processing. Further, when the guide part 220 is inserted into the rewinding groove 150, the end of the guide part 220 away from the rotation axis is located outside the inner side wall of the cup body 100, so that the first opening 1221 can be located on the inner side wall of the cup body 100.

[0041] like Figures 1 to 3 As shown, in some embodiments, the liquid pushing body 210 includes a connecting portion 211 inserted into the conveying groove 140 and surrounding the conveying channel 121 with the groove wall of the conveying groove 140, and a pushing portion 212 connected to the bottom of the connecting portion 211, and the end of the pushing portion 212 away from the connecting portion 211 is connected to the bottom surface of the cup body 100; the guide portion 220 is connected to the connecting portion 211; a liquid gap connecting the first opening 1221 is provided between the pushing portion 212 and the inner side wall of the cup body 100; a preset distance is provided between the side of the pushing portion 212 away from the liquid gap and the rotation axis, and the preset distance is greater than the liquid gap.

[0042] In this embodiment, during the centrifugation of the cup body 100, the target cells in the sample liquid gradually approach the inner wall of the cup body 100, pass through the liquid-passing gap, and finally adhere to the inner wall of the cup body 100, thereby preventing the target cells from being concentrated and accumulated on the liquid-pushing plate 200. In addition, the sample liquid can freely pass through the liquid-passing gap, thereby ensuring that the liquid level heights on both sides of the liquid-pushing plate 200 remain consistent. And setting the preset distance between the liquid-pushing plate 200 and the rotation axis requires providing a certain operating space for the installation and removal of the liquid-pushing plate 200. It is understandable that since the liquid-passing gap should not be set too large, the liquid-pushing plate 200 cannot play a good liquid-pushing effect when the liquid-passing gap is set too large, and the sample liquid near the rotation axis has a small linear velocity during the centrifugation process, and the interval setting of the preset distance has little effect on the centrifugal effect of the sample liquid (and it needs to provide a large operating space), therefore, the preset distance needs to be greater than the liquid-passing gap.

[0043] like Figure 1 As shown, in some embodiments, the cup body 100 further includes a second channel 160 , which is disposed at the bottom of the cup body 100 and communicates with the centrifugal space 110 .

[0044] In this embodiment, the second channel 160 is disposed at the bottom of the cup body 100 and connected to the centrifugal space 110, so that liquid (such as sample liquid or separation liquid, etc.) can enter the centrifugal space 110 from the bottom of the cup body 100 through the second channel 160, thereby preventing splashing of the liquid when it is added to the centrifugal space 110 through the first channel 120, thereby ensuring the stability of the liquid during the process of being added to the centrifugal space 110; in addition, after the sample liquid is centrifuged to obtain a mixed liquid, the mixed liquid can be gathered at the second channel 160 at the bottom of the cup body 100 under the action of gravity, thereby facilitating extraction through the second channel 160, thereby reducing the residual mixed liquid.

[0045] like Figure 1As shown, in some embodiments, the inner wall of the cup body 100 includes a straight wall section 171 and a slope section 172 connected to the bottom of the straight wall section 171, and the first opening 1221 is arranged on the straight wall section 171; one end of the slope section 172 away from the straight wall section 171 is connected to the bottom wall of the cup body 100; along the direction from top to bottom of the rotation axis, the vertical distance between the slope section 172 and the rotation axis gradually decreases. It can be understood that after the liquid other than the target cells is extracted through the first channel 120, the residual liquid (including the target cells) will remain on the chamfered position corresponding to the slope end 172 below the centrifugal space 110 after being mixed, so as to facilitate the maximum extraction from the second channel 160; at the same time, in the process of extracting the liquid other than the target cells through the first channel 120, the liquid other than the target cells in the centrifugal space 110 can also overcome the influence of gravity under the action of centrifugal force, and the acceleration direction is toward the inner wall of the cup body 100. Therefore, the liquid in the slope section 172 will gather toward the straight wall section 171 under the action of centrifugal force. Therefore, the first opening 1221 is set on the straight wall section 171, which can extract the liquid other than the target cells that is not attached to the inner wall of the cup body 100 to the maximum extent, thereby minimizing the liquid residue.

[0046] like Figure 1 As shown, in some embodiments, the second channel 160 includes a third opening 161 disposed on the bottom wall of the cup body 100; the second channel 160 is connected to the centrifugal space 110 through the third opening 161, and one end of the slope section 172 away from the straight wall section 171 is disposed at the opening edge of the third opening 161. It can be understood that the third opening 161 can be disposed at a preset position on the bottom wall of the cup body 100 as needed. In this embodiment, one end of the slope section 172 away from the straight wall section 171 is disposed at the opening edge of the third opening 161, that is, the third opening 161 is at the edge of the bottom wall of the cup body 100, so that when the liquid is drawn through the second channel 160, the liquid can be better guided to the position of the third opening 161 by the slope section 172, further reducing the liquid residue.

[0047] like Figure 1 and Figure 2 As shown, in some embodiments, the cup body 100 includes a cup body 180 with an opening at the top and a cup cover 190 that is detachable (the detachable manner may include but is not limited to a snap connection, etc.) and covers the opening; the cup cover 190 is provided with a first inlet and outlet channel 191 and a second inlet and outlet channel 192 (it can be understood that the specific structural design of the first inlet and outlet channel 191 and the second inlet and outlet channel 192 is not limited to Figure 1As shown in the figure, the flow channel can be specifically designed according to the needs. For example, different control valves can be designed on the first inlet and outlet channel 191 and the second inlet and outlet channel 192 to control the on-off of the flow channel. Pistons can be set at the positions where the first inlet and outlet channel 191 and the second inlet and outlet channel 192 are connected to the cup body 100 or other components to cooperate with sealing or flow channel direction change, which will not be repeated here); the first inlet and outlet channel 191 is connected to the end of the conveying channel 121 away from the bend; the second inlet and outlet channel 192 is connected to the end of the second channel 160 away from the centrifugal space 110; the bend is arranged on the cup cover 190 and / or the cup body 180. It can be understood that the end of the first inlet and outlet channel 191 away from the delivery channel 121 is connected to the outside, and the end of the second inlet and outlet channel 192 away from the second channel 160 is connected to the outside, so that the fluid (for example, fluid such as cell fluid, separation liquid or gas) can be injected into the centrifugal space 110 of the cup body 100 through the first inlet and outlet channel 191 and / or the second inlet and outlet channel 192. It can be understood that the cup body 100 of the cell centrifuge device is in a state of rotating around the rotation axis during the centrifugation process. Therefore, the delivery channel 121 is generally connected to the first inlet and outlet channel 191 at the rotation axis (will not be displaced when the cup body 100 rotates) through a connection method such as a rotation seal, and the second channel 160 is generally connected to the second inlet and outlet channel 192 at the rotation axis (will not be displaced when the cup body 100 rotates) through a connection method such as a rotation seal, so that the first inlet and outlet channel 191 and the second inlet and outlet channel 192 are in a relatively static state during the rotation of the cup body 100, so as to facilitate communication with external pipelines. The curve can be set separately on the cup body 180, or on the cup cover 190, or on both the cup cover 190 and the cup body 180. The inner wall of the cup body 100 may include the inner wall of the cup cover 190 or / and the inner wall of the cup body 180; the curve is formed by connecting the cup cover 190 and the cup body 180.

[0048] Furthermore, the cup cover 190 is provided with an air hole (not shown) connected to the centrifugal space. There is at least one air hole, and a breathable membrane is arranged inside the air hole. Specifically, when the cell centrifuge is used for cell culture, the breathable membrane can prevent external bacteria from entering the centrifugal space. At the same time, the breathable membrane can realize gas exchange between the centrifugal space and the outside world, ensuring the normal respiration of cells in the centrifugal space. Furthermore, the breathable membrane is preferably a hydrophobic breathable membrane.

[0049] In one embodiment, a groove 130 is provided on the inner wall of the cup body 100, and the first opening 1221 is provided on the inner wall of the groove 130. It can be understood that the target cells (the target cells are generally relatively heavy) in the centrifugal space 110 adhere to the inner wall of the cup body 100 under the action of centrifugal force; when the liquid in the centrifugal space 110 is drawn to the inner wall of the cup body 100, due to the viscosity of the liquid, part of the liquid may adhere to the inner wall of the cup body 100. The groove 130 is arranged on the inner wall of the cup body 100, that is, the groove 130 is arranged along the direction of the centrifugal force. Then, after the liquid in the centrifugal space 110 is extracted to the inner wall of the cup body 100, the liquid adhering to the inner wall of the cup body 100 will continue to gather in the groove 130; the first opening 1221 is arranged on the inner wall of the groove 130, so that the liquid gathered in the groove 130 can be more extracted through the first opening 1221 arranged on the inner wall of the groove 130, thereby further reducing the minimum concentration volume. Further, the first opening 1221 is arranged on the inner wall of the groove 130 away from the end of the rotation axis, so that the liquid in the centrifugal space 110 can be extracted to the maximum extent. The groove 130 can be arranged according to actual conditions, for example: a rectangular groove (gradient structure), a conical groove (continuous structure) or a spherical groove, etc., and the groove 130 can be arranged on the cup cover or / and the cup body in the cup body.

[0050] like Figure 4 As shown, one embodiment of the present invention further provides a cell centrifugation method, which is performed by the cell centrifugation device, and the cell centrifugation method comprises:

[0051] S10, controlling the first preset dose of separation liquid to flow into the centrifugal space 110 in the cup body 100 of the cell centrifuge device. In one embodiment, by controlling the peristaltic pump to rotate (clockwise or counterclockwise, or other liquid pumps) to allow the separation liquid to flow into the centrifugal space 110, the first preset dose of separation liquid can be accurately controlled to flow into the centrifugal space 110 in the cup body 100 of the cell centrifuge device. The first preset dose of separation liquid can flow into the centrifugal space 110 in the cup body 100 of the cell centrifuge device through the first channel 120 (or the second channel 160). The first preset dose can be set according to actual conditions, as long as it plays a buffering role when the sample liquid enters the centrifugal space 110 in the cup body 100, for example, considering the length of the pipeline or the capacity of the centrifugal space 110 in the cup body 100. In one embodiment, the first preset dose is 120mL-150mL, so as to ensure that the separation liquid enters the centrifugal space 110 in the cup body 100 through the pipeline and acts as a buffer for the sample liquid entering the centrifugal space 110 in the cup body 100 in step S20. It can be understood that in step S10, the cell centrifuge device can also inject the separation liquid after starting the cup body 100 to rotate (the specific speed can be set according to demand, such as being set to the same as the first preset speed. In this case, the first preset speed can be maintained in step S20 without being turned on again).

[0052] S20, after controlling the cup body 100 to rotate around the rotation axis at a first preset speed, controlling the second preset dose of sample liquid to flow into the centrifugal space 110 at a preset flow rate. It can be understood that controlling the cup body 100 to rotate around the rotation axis at the first preset speed is to control the cup body 100 to start the centrifugal operation. It can be understood that controlling the cup body 100 to rotate around the rotation axis at the first preset speed can be before step S10, that is, controlling the cup body 100 to rotate around the rotation axis at the first preset speed, and then controlling the first preset dose of separation liquid to flow into the centrifugal space 110 in the cup body 100 of the cell centrifuge device; controlling the cup body 100 to rotate around the rotation axis at the first preset speed can also be after step S10, or simultaneously with step S10, thereby saving processing time. The range of the preset flow rate can be set to 0-300mL / min, and the realization of the preset flow rate can be achieved by squeezing the pipeline by rotating a non-contact peristaltic pump. In this embodiment, the cup body 100 is first controlled to rotate around the rotation axis at a first preset speed, and then the sample liquid of the second preset dose is controlled to flow into the centrifugal space 110 at a preset flow rate, so that the sample liquid can be centrifuged at the same time as entering the centrifugal space 110, thereby saving processing time and improving processing efficiency. The first preset speed can be set according to actual conditions, so as to achieve the best centrifugal effect according to different types of sample liquid. In one embodiment, the first preset speed is in the range of 2000rpm-2500rpm (Revolutions Per minute, revolutions per minute), so as to achieve the best centrifugal effect for the sample liquid containing PBMC cells (peripheral blood mononuclear cells, peripheral blood mononuclear cells) (the PBMC cells in the sample liquid are the target cells). The second preset dose can be set according to actual conditions, generally considering factors such as the capacity of the sample liquid bag or the size of the centrifugal space 110. In one embodiment, the second preset dose is 150mL-200mL.

[0053] S30, control the cup body 100 to rotate at a second preset speed for a first preset time to centrifuge the liquid in the centrifugal space 110 in the cup body 100, so that the target cells in the sample liquid are close to the inner wall of the cup body 100. It can be understood that after the first preset dose of separation liquid and the second preset dose of sample liquid are injected into the centrifugal space 110, the liquid in the centrifugal space 110 can be continuously centrifuged. The first preset time can be set according to actual conditions, so as to achieve the best centrifugal effect according to different sample liquid types or doses. In one embodiment, the first preset time is 150 seconds, so that the best centrifugal effect can be achieved for a sample liquid of preferably 150 mL. The second preset speed can be set according to actual conditions (the second preset speed can be the same as or different from the first preset speed), so as to achieve the best centrifugal effect according to different sample liquid types. In one embodiment, the second preset speed is preferably 2000 rpm, so that the best centrifugal effect can be achieved for a sample liquid containing PBMC cells (target cells).

[0054] S40, the liquid except the target cells after the centrifugal operation is discharged from the centrifugal space 110 through the first opening 1221 of the first channel 120, the bend 122 and the delivery channel 121. It can be understood that the cup body 100 rotates continuously, the target cells in the sample liquid (the target cells are generally relatively heavy, such as PBMC cells) are close to the inner wall of the cup body 100, and the bending channel 1223 is located on the outer side of the inner wall of the cup body 100, that is, the bending channel 1223 is bent in the direction away from the inner wall of the cup body 100, and the bending channel 1223 is located between the inner wall and the outer wall of the cup body 100; and the first opening 1221 is located on the inner wall. When the liquid in the centrifugal space 110 is extracted through the first channel 120, the liquid will enter the bending channel 1221 from the first opening 1221. 23. Since the bending channel 1223 is bent in a direction away from the inner wall of the cup body 100, the flow direction of the fluid entering the bending channel 1223 from the first opening 1221 will be from the inner wall to the outer wall of the cup body 100 (basically no or very little thrust from bottom to top will be generated). Therefore, in the entire process of extracting liquid through the first channel 120, since the target cells have been pressed and attached to the inner wall of the cup body 100 under the action of centrifugal force, and there is basically no thrust from bottom to top, the extraction of liquid through the first channel 120 will minimize the target cells from being extracted synchronously. At the same time, when the liquid in the centrifugal space 110 is extracted through the first channel 120, since the cup body 100 of the cell centrifuge device is in a state of rotating around the rotation axis during the centrifugation process, the liquid in the centrifugal space 110 except the target cells will also be attached to the inner wall of the cup body 100 under the action of centrifugal force. Therefore, the first opening 1221 is disposed on the inner wall of the cup body 100 , and can extract the liquid other than the target cells that is not attached to the inner wall of the cup body 100 to the maximum extent, thereby minimizing liquid residue.

[0055] Therefore, in the above embodiment of the present invention, by designing the bend 122 and the delivery channel 121 on the cup body 100, while avoiding the extraction of target cells as much as possible, other liquids except the target cells attached to the inner wall of the cup body 100 can be extracted to the maximum extent, thereby minimizing the liquid residue, reducing the minimum concentration volume, reducing the cost, and improving the cell centrifugation efficiency.

[0056] like Figure 5 As shown, in some embodiments, in the step S20, after the second preset dose of sample liquid is controlled to flow into the centrifugal space 110 at a preset flow rate, the step further includes:

[0057] S50, controlling the third preset dose of separation liquid to flow into the centrifugal space 110. It is understandable that in step S20, part of the sample liquid will remain in the pipeline before entering the centrifugal space 110. In order to avoid waste of sample liquid, in this embodiment, by controlling the third preset dose of separation liquid to flow into the centrifugal space 110, the sample liquid remaining in the pipeline can be pushed into the centrifugal space 110. The third preset dose is set according to actual conditions, as long as all the sample liquid remaining in the pipeline is pushed into the centrifugal space 110. In one embodiment, the third preset dose is 30mL-40mL.

[0058] like Figure 6 As shown, in some embodiments, the cup body 100 further includes a second channel 160, which is disposed at the bottom of the cup body 100 and communicates with the centrifugal space 110; the step S40, after the liquid except the target cells after the centrifugal operation is discharged from the centrifugal space 110 through the first opening 1221, the bend 122 and the delivery channel 121 in sequence, further includes:

[0059] S60, when it is detected that the liquid discharged from the first channel 120 has been exhausted, control the target base liquid to flow into the centrifugal space 110. The target base liquid includes but is not limited to at least one of a cleaning liquid, a freezing liquid or a culture medium.

[0060] S70, control the cup body 100 to rotate at a third preset speed for a second preset time, so as to evenly mix the target cells in the centrifugal space 110 with the target base liquid to obtain a mixed liquid. It can be understood that the third preset speed can be set according to actual conditions, so as to achieve the best mixing effect according to different types of sample liquids. In one embodiment, the third preset speed is 2000rpm, so as to achieve the best mixing effect for the sample liquid containing PBMC cells. The second preset time can be set according to actual conditions, so as to achieve the best mixing effect according to different types of sample liquids. In one embodiment, the second preset time is 210 seconds, so as to achieve the best mixing effect for the sample liquid containing PBMC cells.

[0061] S80, discharge the mixed liquid in the centrifugal space 110 into a preset storage container through the second channel 160. It can be understood that in this step, the cup body 100 stops rotating, and the mixed liquid in the centrifugal space 110 falls to the bottom of the cup body 100 under the action of gravity. The second channel 160 is arranged at the bottom of the cup body 100, and the mixed liquid can be extracted from the bottom of the cup body 100 to the maximum extent, avoiding residual mixed liquid and waste of cost.

[0062] It is understandable that the above-mentioned cell centrifugation method is not limited to that described in the above-mentioned embodiments. The specific configuration of the cell centrifugation method of the present application corresponds one-to-one with the above-mentioned cell centrifugation device, and will not be described in detail here.

[0063] An embodiment of the present invention further provides a controller, the controller comprising a processor and a memory, the memory storing an executable program, and the processor being used to execute the executable program to implement the cell centrifugation method.

[0064] The specific configuration of the controller of the present application corresponds to the above-mentioned cell centrifugation method one by one, and will not be repeated here. Each module in the above-mentioned controller can be implemented in whole or in part by software, hardware and a combination thereof. Each of the above-mentioned modules can be embedded in or independent of the controller in the form of hardware, or can be stored in the controller in the form of software, so that the controller can call and execute the operations corresponding to each of the above modules.

[0065] An embodiment of the present invention further provides a computer-readable storage medium, wherein the computer-readable storage medium stores a computer program, and when the computer program is executed by a processor, the above-mentioned cell centrifugation method is implemented.

[0066] Those of ordinary skill in the art can understand that all or part of the processes in the above-mentioned embodiment methods can be implemented by instructing related hardware through computer-readable instructions, and the computer-readable instructions can be stored in a non-volatile readable storage medium or a volatile readable storage medium. When the computer-readable instructions are executed, they may include the processes of the embodiments of the above-mentioned methods. Among them, any reference to memory, storage, database or other media used in the embodiments provided in this application may include non-volatile and / or volatile memory. Non-volatile memory may include read-only memory (ROM), programmable ROM (PROM), electrically programmable ROM (EPROM), electrically erasable programmable ROM (EEPROM) or flash memory. Volatile memory may include random access memory (RAM) or external cache memory. By way of illustration and not limitation, RAM is available in many forms, such as static RAM (SRAM), dynamic RAM (DRAM), synchronous DRAM (SDRAM), double data rate SDRAM (DDRSDRAM), enhanced SDRAM (ESDRAM), synchronous link (Synchlink) DRAM (SLDRAM), memory bus (Rambus) direct RAM (RDRAM), direct memory bus dynamic RAM (DRDRAM), and memory bus dynamic RAM (RDRAM), etc.

[0067] Those skilled in the art will clearly understand that for the sake of convenience and brevity of description, only the division of the above-mentioned functional units and modules is used as an example. In actual applications, the above-mentioned functions can be distributed and completed by different functional units and modules as needed, that is, the internal structure of the system can be divided into different functional units or modules to complete all or part of the functions described above.

[0068] The above are merely embodiments of the cell centrifuge device, method, controller and medium of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell centrifuge device, characterized in that: The invention comprises a cup body provided with a centrifugal space and a first channel; the cup body has a rotation axis, and the cup body can rotate around the rotation axis; The first channel includes a conveying channel arranged at the top of the cup body, and a bend arranged on the side wall of the cup body; a first opening and a second opening are respectively provided at both ends of the bend; the first opening is arranged on the inner wall of the cup body; the bend is connected to the centrifugal space through the first opening, and the bend is connected to the conveying channel through the second opening; the bend also includes at least one bending channel located outside the inner wall of the cup body.

2. The cell centrifuge device according to claim 1, characterized in that A first distance between the inflection point of the bending channel and the rotation axis is greater than a second distance between the inner side wall of the cup body and the rotation axis.

3. The cell centrifuge device according to claim 1, characterized in that The bending channel includes a U-shaped bending channel or a J-shaped bending channel.

4. The cell centrifuge device according to claim 1, characterized in that The cell centrifuge device also includes a liquid pushing plate installed on the cup body and located in the centrifugal space; When the cup body rotates around the rotation axis, the liquid pushing plate rotates synchronously with the cup body, thereby pushing the liquid to rotate synchronously in the same rotation direction as the cup body.

5. The cell centrifuge device according to claim 4, characterized in that The top of the cup body is provided with a conveying groove, and the side wall of the cup body is provided with a winding groove connected to the conveying groove; The liquid pushing plate comprises a liquid pushing body and a flow guide portion connected to a side of the liquid pushing body away from the rotation axis; The top end of the liquid pushing body is inserted into the conveying groove and forms the conveying channel with the groove wall of the conveying groove, and the bottom end of the liquid pushing body is connected to the bottom surface of the cup body; the guide part is inserted into the winding groove and forms the bend with the winding groove.

6. The cell centrifuge device according to claim 5, characterized in that The liquid pushing body includes a connecting part which is inserted into the conveying groove and forms the conveying channel with the groove wall of the conveying groove, and a pushing part connected below the connecting part, and the end of the pushing part away from the connecting part is connected to the bottom surface of the cup body; the guide part is connected to the connecting part; a liquid passing gap connected to the first opening is provided between the pushing part and the inner side wall of the cup body; a preset distance is set between the side of the pushing part away from the liquid passing gap and the rotation axis, and the preset distance is greater than the liquid passing gap.

7. The cell centrifuge device according to any one of claims 1 to 6, characterized in that: The cup body further comprises a second channel, which is arranged at the bottom of the cup body and communicates with the centrifugal space.

8. The cell centrifuge device according to claim 7, characterized in that The inner side wall of the cup body comprises a straight wall section and a slope section connected below the straight wall section, the first opening is arranged on the straight wall section; one end of the slope section away from the straight wall section is connected to the bottom wall of the cup body; Along the direction from top to bottom of the rotation axis, the vertical distance between the slope section and the rotation axis gradually decreases.

9. The cell centrifuge device according to claim 8, characterized in that The second channel includes a third opening arranged on the bottom wall of the cup body; the second channel is connected to the centrifugal space through the third opening, and one end of the slope section away from the straight wall section is arranged at the opening edge of the third opening.

10. The cell centrifuge device according to claim 7, characterized in that The cup body includes a cup body with an opening at the top and a cup cover that is detachably covered on the opening; the cup cover is provided with a first inlet and outlet channel and a second inlet and outlet channel; the first inlet and outlet channel is connected to an end of the conveying channel away from the bend; the second inlet and outlet channel is connected to an end of the second channel away from the centrifugal space; the bend is arranged on the cup cover and / or the cup body.

11. The cell centrifuge device according to claim 1, characterized in that A groove is provided on the inner side wall of the cup body, and the first opening is arranged on the inner wall of the groove.

12. A cell centrifugation method, characterized in that: The cell centrifugation method is performed by a cell centrifugation device according to any one of claims 1 to 11, and the cell centrifugation method comprises: Controlling a first preset dose of separation liquid to flow into the centrifugal space in the cup body of the cell centrifuge device; After controlling the cup body to rotate around the rotation axis at a first preset speed, controlling a second preset dose of sample liquid to flow into the centrifugal space at a preset flow rate; Controlling the cup body to rotate at a second preset speed for a first preset time to centrifuge the liquid in the centrifugal space in the cup body, so that the target cells in the sample liquid are closely attached to the inner wall of the cup body; After the centrifugal operation, the liquid except the target cells is discharged from the centrifugal space through the first opening of the first channel, the bend and the transport channel in sequence.

13. The cell centrifugation method according to claim 12, characterized in that: After controlling the second preset dose of sample liquid to flow into the centrifugal space at a preset flow rate, the method further includes: A third preset dose of separation liquid is controlled to flow into the centrifugal space.

14. The cell centrifugation method according to claim 12, characterized in that: The cup body further comprises a second channel, which is arranged at the bottom of the cup body and communicates with the centrifugal space; After the liquid except the target cells after the centrifugal operation is discharged from the centrifugal space through the first opening, the bend and the delivery channel in sequence, the method further comprises: When it is detected that the liquid discharged from the first channel has been exhausted, controlling the target base liquid to flow into the centrifugal space; Controlling the cup body to rotate at a third preset speed for a second preset time to evenly mix the target cells in the centrifugal space with the target base liquid to obtain a mixed liquid; The mixed liquid in the centrifugal space is discharged into a preset storage container through the second channel.

15. A controller, characterized in that: The controller comprises a processor and a memory, wherein the memory stores an executable program, and the processor is configured to execute the executable program to implement the cell centrifugation method according to any one of claims 12 to 14.

16. A computer-readable storage medium storing a computer program, characterized in that: When the computer program is executed by a processor, the cell centrifugation method according to any one of claims 12 to 14 is implemented.

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